Sean Jung, Samira Dahesh, Elisabet Bjånes, Jonathan Monk, Natalie Chavarria, Sachiko Correa, Alexandria Hoffman, Hunter Gage, Victor Nizet, Monika Kumaraswamy
{"title":"Investigating Azithromycin Activity Against ESBL-Producing Escherichia coli Under Physiologically Relevant Conditions","authors":"Sean Jung, Samira Dahesh, Elisabet Bjånes, Jonathan Monk, Natalie Chavarria, Sachiko Correa, Alexandria Hoffman, Hunter Gage, Victor Nizet, Monika Kumaraswamy","doi":"10.1093/infdis/jiaf480","DOIUrl":"https://doi.org/10.1093/infdis/jiaf480","url":null,"abstract":"Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are a major antimicrobial resistance threat. Although not standard therapy, azithromycin (AZM) displayed potent activity against ESBL E. coli in vitro, ex vivo, and in vivo. AZM demonstrated multi-fold reductions in MIC, bactericidal activity in supplemented mammalian tissue culture media, and enhanced dose-dependent activity with sodium bicarbonate (NaHCO3). AZM also augmented complement-mediated killing in human serum and improved survival by 50% in a murine bloodstream infection model. These findings underscore the need to revisit antibiotic susceptibility testing—incorporating host defense factors and NaHCO3—and suggest AZM merits further clinical evaluation for ESBL E. coli infections.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"76 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145089603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Neval Ete Wareham,Victoria S Kjærgaard,Mohammed Habib,Joanne Reekie,Kasper Rossing,Henrik Sengeløv,Marlyse Buisson,Jean-Luc Lenormand,Michael Perch,Nicolai Schultz,Nikolai Kirkby,Søren S Sørensen,Julien Lupo,Jens D Lundgren,Daniel D Murray,Emmanuel Drouet
{"title":"The diagnostic value of soluble Epstein-Barr Virus BZLF1 protein (sZEBRA) in the Detection of Post-transplant lymphoproliferative Disease (PTLD) among Solid Organ (SOT) and Haematopoietic Cell (HSCT) Transplant Recipients.","authors":"Neval Ete Wareham,Victoria S Kjærgaard,Mohammed Habib,Joanne Reekie,Kasper Rossing,Henrik Sengeløv,Marlyse Buisson,Jean-Luc Lenormand,Michael Perch,Nicolai Schultz,Nikolai Kirkby,Søren S Sørensen,Julien Lupo,Jens D Lundgren,Daniel D Murray,Emmanuel Drouet","doi":"10.1093/infdis/jiaf477","DOIUrl":"https://doi.org/10.1093/infdis/jiaf477","url":null,"abstract":"BACKGROUNDPost-transplant lymphoproliferative disorder (PTLD) is a serious complication of solid organ transplant (SOT) and hematopoietic stem cell transplant (HSCT) recipients and is often associated with Epstein-Barr virus (EBV) reactivation. The sZEBRA protein, a soluble form of the EBV nuclear Immediate-early antigen BZLF1 (also called Zta), plays a crucial role in EBV reactivation and immune evasion. This study aimed to externally validate an association between sZEBRA and the diagnosis of PTLD.METHODSIn this retrospective case-control study, the relative odds of having PTLD according to sZEBRA plasma levels at diagnosis of PTLD and comparable follow-up for controls was analyzed using logistic regression adjusting for demographics, transplant information and sample timing. The model was further also adjusted for corresponding EBV PCR plasma levels. Levels of sZEBRA was fitted in the model either as present/absent or in quartiles.RESULTS33 (17%) PTLD cases and 161 (83%) controls were included. The adjusted odds ratio (aOR) of a positive versus negative sZEBRA test for PTLD diagnosis was 2.82 (95% CI 1.37-7.68); after additional adjustment for EBV PCR levels, the aOR was 4.15 (95% CI 1.31-13.14). Separating sZEBRA levels into quartiles, aOR of sZEBRA in the 4th quartile was 12.43 (95% CI 1.99-77.55) compared to a negative result in the fully adjusted model.CONCLUSIONElevated sZEBRA levels, especially those in the highest quartile and when combined with EBV PCR, were associated with PTLD and may serve as a complementary biomarker to EBV PCR to identify patients with PTLD.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145083310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Prakruti S Rao,Senbagavalli Prakash Babu,Komala Ezhumalai,Selby Knudsen,Jayalakshmy Ramakrishnan,Noyal Joseph,Chelsie Cintron,Madolyn R Dauphinais,Prakash Babu Narasimhan,Padmini Salgame,Natasha S Hochberg,David L Hom,Scott K Heysell,Charles Robert Horsburgh,Jerrold J Ellner,Pranay Sinha,Sonali Sarkar
{"title":"Rifampin Exposure in Pregnant and Non-Pregnant Women with Tuberculosis in India.","authors":"Prakruti S Rao,Senbagavalli Prakash Babu,Komala Ezhumalai,Selby Knudsen,Jayalakshmy Ramakrishnan,Noyal Joseph,Chelsie Cintron,Madolyn R Dauphinais,Prakash Babu Narasimhan,Padmini Salgame,Natasha S Hochberg,David L Hom,Scott K Heysell,Charles Robert Horsburgh,Jerrold J Ellner,Pranay Sinha,Sonali Sarkar","doi":"10.1093/infdis/jiaf467","DOIUrl":"https://doi.org/10.1093/infdis/jiaf467","url":null,"abstract":"This prospective cohort study evaluated rifampin pharmacokinetics in pregnant and non-pregnant women with tuberculosis in India. Pregnant women had significantly lower drug exposure, with <20% of the participants achieving target concentrations at any trimester. Findings highlight potential underdosing in pregnancy and underscore the need for dedicated pharmacokinetics studies in pregnancy, and revised rifampin dosing guidelines.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marta Bermejo-Jambrina,Viktoria Zaderer,Julia Eder,Gabriel Diem,Killian E Vlaming,Wilfried Posch,Teunis B H Geijtenbeek,Doris Wilflingseder
{"title":"The Role of Enoxaparin in Influenza Virus Infections and Its Therapeutic Implications.","authors":"Marta Bermejo-Jambrina,Viktoria Zaderer,Julia Eder,Gabriel Diem,Killian E Vlaming,Wilfried Posch,Teunis B H Geijtenbeek,Doris Wilflingseder","doi":"10.1093/infdis/jiaf470","DOIUrl":"https://doi.org/10.1093/infdis/jiaf470","url":null,"abstract":"Frequent emergence of respiratory viruses with pandemic potential, like SARS-CoV-2 or influenza, underscores the need for broad-spectrum prophylaxis. Existing vaccines show reduced efficacy against newly emerged variants, and the ongoing risk of new outbreaks highlights the importance of alternative strategies to prevent infection and viral transmission. As respiratory viruses primarily enter through the nose, formulations targeting the nasal epithelium are attractive candidates to neutralize pathogens and thus prevent or minimize infection. Enoxaparin, a low molecular weight heparin (LMWH) widely used as an anticoagulant, also exhibits antiviral and anti-inflammatory properties. We found that in highly-differentiated human nasal and upper respiratory tract 3D-models, enoxaparin inhibited influenza_A/H3N2 and B/Victoria infection, reduced release of pro-inflammatory cytokines and chemokines, and mitigated epithelial damage caused by infection. Our study hihlights the LMWH inhibitory effect on respiratory viruses. When applied to mucosal entry sites, LMWH shows promise as prophylactic and valuable alternative to traditional antiviral approaches.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fatou Joof,Ruoqian Hu,Karl B Seydel,Lauren M Cohee,Ying Zheng,Joseph D Smith
{"title":"Febrile temperature augments ring-stage Plasmodium falciparum adhesion to brain endothelial cells.","authors":"Fatou Joof,Ruoqian Hu,Karl B Seydel,Lauren M Cohee,Ying Zheng,Joseph D Smith","doi":"10.1093/infdis/jiaf474","DOIUrl":"https://doi.org/10.1093/infdis/jiaf474","url":null,"abstract":"Sequestration of Plasmodium falciparum-infected erythrocytes (IE) in the microvasculature is a major virulence determinant. While the sequestration of mature stage parasites (trophozoite and schizonts) to vascular endothelium is well established, the conditions that promote ring-stage IE sequestration is less understood. Here, we observed in ring-stage parasites that febrile exposure increased transcript levels of several exported parasite genes involved in the trafficking of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) ligand responsible for adherence to the endothelium of blood vessels. Furthermore, it accelerated PfEMP1 surface display in ring-stage IEs, leading to a twofold increase in their binding in a perfusable 3D human brain microvessel model. Additionally, we observed that parasite exposure enhances the binding of uninfected erythrocytes (UE) in 3D brain microvessels. These findings suggest a complex interplay between fever and parasite biomass in the pathogenesis of cerebral malaria.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145031832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shihui Jin,Toshiaki R Asakura,Hiroaki Murayama,Sung-Mok Jung,David Niyukuri,Joseph Nyandwi,Liliane Nkengurutse,Olivier Kamatari,Jue Tao Lim,Akira Endo,Borame L Dickens
{"title":"Disentangling temporal trends of clade Ib monkeypox virus transmission in Burundi.","authors":"Shihui Jin,Toshiaki R Asakura,Hiroaki Murayama,Sung-Mok Jung,David Niyukuri,Joseph Nyandwi,Liliane Nkengurutse,Olivier Kamatari,Jue Tao Lim,Akira Endo,Borame L Dickens","doi":"10.1093/infdis/jiaf475","DOIUrl":"https://doi.org/10.1093/infdis/jiaf475","url":null,"abstract":"Utilising mpox case data from Burundi between August 2024 and April 2025, we calibrated a mathematical model to quantify the temporal trends of clade Ib monkeypox virus transmission. The model outputs indicated a declining overall transmission trend. Children aged 0-4 and 5-9 years were estimated to be at higher risk of infection compared to older age groups, while sexual contact was inferred to contribute up to 50% of the overall transmission.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jacklyn R Hurst,Mary Addo,Abby Li,Shreya S Khera,Reva Persaud,Cassandra Bertucci,Misha Hummel,Oscar Javier Pico Espinosa,Adrienne K Chan,Sharon Walmsley,Sharmistha Mishra,Darrell H S Tan,Robert Kozak
{"title":"Monkeypox virus shedding despite tecovirimat treatment in a cohort in Toronto, Canada.","authors":"Jacklyn R Hurst,Mary Addo,Abby Li,Shreya S Khera,Reva Persaud,Cassandra Bertucci,Misha Hummel,Oscar Javier Pico Espinosa,Adrienne K Chan,Sharon Walmsley,Sharmistha Mishra,Darrell H S Tan,Robert Kozak","doi":"10.1093/infdis/jiaf471","DOIUrl":"https://doi.org/10.1093/infdis/jiaf471","url":null,"abstract":"BACKGROUNDTecovirimat (TPOXX) is an antiviral authorized for the treatment of mpox infections in Canada, but recent clinical trials found it has no impact on symptom duration.METHODSWe conducted a prospective cohort study of individuals diagnosed with mpox in Toronto, Canada. Skin lesion swabs were collected weekly to quantify infectious monkeypox virus (MPXV) shedding through cell culture. The presence of antiviral resistance mutations was assessed by PCR and sequencing the F13L gene.RESULTSAmong 17 participants, 9 received tecovirimat, with a median initiation time of 14 days post-symptom onset. Infectious MPXV was detected in 31% (17/55) of lesion swabs from tecovirimat-treated participants and 32% (20/62) from untreated individuals. Shedding kinetics were similar between groups, with persistent infectious virus detected in several participants beyond two weeks of symptoms. Despite more than 7 days after tecovirimat initiation, four treated participants still shed viable virus in at least one sampled lesion, including up to 15 days after tecovirimat initiation. No known resistance mutations were identified in viral sequences from a subset of lesion swabs from both treated and untreated individuals, suggesting that tecovirimat resistance mutations were not widely circulating in Toronto during the 2022 outbreak.CONCLUSIONOur findings suggest that tecovirimat does not significantly impact the duration of infectious MPXV shedding from skin lesions, aligning with recent randomized trial results. These findings highlight the need for alternative antiviral strategies and continued genomic surveillance to monitor resistance emergence.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145025460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benjamin Orlinick, Sameet Mehta, Lindsay McAlpine, Saba Khoshbakht, Sofia Fertuzinhos, Allison Nelson, Jennifer Chiarella, Bibhuprasad Das, Vansh Patel, Paraskevas Filippidis, Michael J Corley, Serena S Spudich, Shelli F Farhadian
{"title":"CSF immune cell alterations in women with neuropsychiatric Long COVID","authors":"Benjamin Orlinick, Sameet Mehta, Lindsay McAlpine, Saba Khoshbakht, Sofia Fertuzinhos, Allison Nelson, Jennifer Chiarella, Bibhuprasad Das, Vansh Patel, Paraskevas Filippidis, Michael J Corley, Serena S Spudich, Shelli F Farhadian","doi":"10.1093/infdis/jiaf468","DOIUrl":"https://doi.org/10.1093/infdis/jiaf468","url":null,"abstract":"Background Women are disproportionately affected by neuropsychiatric symptoms following recovery from acute COVID-19. However, whether there are central nervous system-specific changes in gene expression in women with neuropsychiatric Long COVID (NP-Long COVID) remains unknown. Methods Twenty-two women with and ten women without NP-Long COVID were enrolled from New Haven, CT, and the surrounding region and consented to a blood draw and large volume lumbar puncture. Total RNA was extracted from cerebrospinal fluid (CSF) cells and peripheral blood mononuclear cells (PBMC). Polyadenylated RNA was sequenced, and differential expression analyses were performed. Results Both CSF and PBMC samples showed differential gene expression associated with Long COVID status. There were CSF-specific differentially expressed genes (DEGs) in people with Long COVID, including in genes related to oxidative stress, reactive oxygen species, and P53 response, indicating compartment-specific immune responses. Some pathways were dysregulated in both the CSF and PBMC of Long COVID compared to controls, including those related to androgen response, MTORC1 signaling, and lipid metabolism. Conclusions Women with NP-long COVID show compartment-specific, transcriptional profiles in the CSF with evidence of enrichment in cellular stress pathways. These results underscore the importance of examining CSF-specific molecular profiles to better understand post-viral neurological syndromes.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145017574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gene editing of a carcinogenic liver fluke tetraspanin impairs parasite surface biogenesis and extracellular vesicle uptake by human host cells.","authors":"Sujittra Chaiyadet,Wannaporn Ittiprasert,Michael J Smout,Ladawan Khowawisetsut,Apisit Ruangsuwast,Paul J Brindley,Alex Loukas,Thewarach Laha","doi":"10.1093/infdis/jiaf466","DOIUrl":"https://doi.org/10.1093/infdis/jiaf466","url":null,"abstract":"Opisthorchiasis remains a significant public health concern throughout Southeast Asia. The liver fluke Opisthorchis viverrini resides within the biliary tract and chronic infection leads to bile duct cancer, or cholangiocarcinoma. Here, we examined the functions of liver fluke tetraspanins, four-transmembrane domain proteins expressed on the surface of the fluke tegument and extracellular vesicles (EVs) derived from this syncytial surface. We undertook CRISPR-Cas9 gene knockout (KO) of the O. viverrini tetraspanin-2 (Ov-tsp-2) gene and found that Ov-tsp-2-KO flukes had abnormal tegument biogenesis. The tegument of Ov-tsp-2-KO flukes was increasingly vacuolated and fewer EVs were secreted. EVs that were secreted were deficient in Ov-TSP-2 and their uptake by cholangiocytes was diminished. The findings indicate a critical role for Ov-TSP-2 in maintenance of the tegument, EV production and uptake by host target cells, and support the development of this parasite antigen as an anti-infection and anti-cancer vaccine for opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144962719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nathalia Rammé M de Albuquerque,Rodrigo M Corder,Igor C Johansen,Winni A Ladeia,Priscila T Rodrigues,Simone Ladeia-Andrade,Joana C Silva,Marcelo U Ferreira
{"title":"The source-sink dynamics of Plasmodium vivax may undermine malaria elimination efforts in the Amazon: an epidemiological and population genomic study.","authors":"Nathalia Rammé M de Albuquerque,Rodrigo M Corder,Igor C Johansen,Winni A Ladeia,Priscila T Rodrigues,Simone Ladeia-Andrade,Joana C Silva,Marcelo U Ferreira","doi":"10.1093/infdis/jiaf457","DOIUrl":"https://doi.org/10.1093/infdis/jiaf457","url":null,"abstract":"BACKGROUNDBrazil's progress toward malaria elimination has stalled and 163,000 new cases (more than 80% caused by Plasmodium vivax) were recorded in the Brazilian Amazon in 2023. We hypothesize that human mobility continues to disperse parasites from hotspots to areas with decreasing endemicity.METHODSWe analyzed 5.5 million malaria case notifications between 2003 and 2023 to describe malaria case mobility and identify sources and sinks of P. vivax in the Brazilian Amazon. We leveraged whole-genome sequence data from 408 P. vivax isolates sampled from across South America to characterize parasite gene flow and infer likely regional routes of parasite dispersal.RESULTSWe found that nearly one-third of the P. vivax infections diagnosed in residents in the Brazilian Amazon over 21 years were acquired outside the locality or municipality of residence, but only 1.7% were imported from other countries in South America, mostly from the Guiana Shield. We show that large cities with residual malaria transmission - such as Manaus and Porto Velho - are receptive parasite sinks surrounded by high-risk source rural localities. Although the genetic relatedness of parasites tended to decrease with geographic distance, parasites from sites more than 1,000 km apart often remained genetically connected.CONCLUSIONSUnderstanding parasite source-sink dynamics on different geographic scales is crucial to target high-risk mobile populations and source localities along with receptive sinks within low-transmission municipalities, with the goal of eliminating malaria transmission and preventing its reintroduction into malaria-free areas across the Amazon.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144960265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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