Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Efficacy of two different forms of selenium towards reduction of arsenic toxicity and accumulation in Cicer arietinum L.","authors":"Naba Kumar Mondal ,&nbsp;Barnali Mondal ,&nbsp;Rajesh Koley ,&nbsp;Apurba Koley ,&nbsp;Srinivasan Balachandran","doi":"10.1016/j.jtemb.2024.127541","DOIUrl":"10.1016/j.jtemb.2024.127541","url":null,"abstract":"<div><div>Arsenic migration from soil to crop plants and subsequently human consumption of contaminated foodstuffs is a serious threat for society. In the present study, two oxidation states of selenium [Se(0) and Se(VI)] were used to check their efficacy towards amelioration of arsenic toxicity in chickpeas (<em>Cicer arietinum</em> L.). Three different concentrations (1, 5, and 10 mg/L) of both oxidation states of selenium were applied separately and in combination against a fixed dose (10 mg/L) of arsenic [(As(V)] treatment on chickpea seedlings. Further, seed germination and seedling growth attributes, oxidative stress, and antioxidant defense under different treatments were analyzed. The changes in anatomical structures and arsenic accumulation in different parts of seedlings were also studied. Results revealed that increased generation of oxidative stress affected physiobiochemical parameters of seedlings and diminished plant growth and deformation in vascular bundles under arsenic stress. However, the combined application of Se with As showed overall improvement in seedling growth, reduced oxidative stress, and organized vascular bundles of chickpea seedlings as compared to arsenic stress alone. The arsenic uptake and accumulation in chickpea seedlings were also reduced upon supplementation of Se. The highest reduction of arsenic accumulation by 42 and 56 % in roots, while 47 and 58 % in shoots were recorded by the application of 10 mg/L of Se(0) and Se (VI) under As stress, respectively. Overall, Se(VI) showed much better performance towards the minimization of arsenic-induced phytotoxicity and arsenic accumulation as compared to Se(0). Therefore, this study explored the efficacy of different forms of selenium towards the mitigation of arsenic toxicity in plants.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127541"},"PeriodicalIF":3.6,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of multiple urinary metals/metalloids with obesity defined by body fat percentage: A cross-sectional study among Guangxi Zhuang ethnic in China","authors":"Fangfang Lv ,&nbsp;Lidi Lei ,&nbsp;Gangjie Wei ,&nbsp;Qunjiao Jiang ,&nbsp;Caimei Mo ,&nbsp;Jinxiu Li ,&nbsp;Peini Lu ,&nbsp;Xuemei Xu ,&nbsp;Xuanqian Huang ,&nbsp;Dongping Huang ,&nbsp;Li Su ,&nbsp;Xiaoqiang Qiu ,&nbsp;Xiaoyun Zeng ,&nbsp;Shun Liu","doi":"10.1016/j.jtemb.2024.127538","DOIUrl":"10.1016/j.jtemb.2024.127538","url":null,"abstract":"<div><h3>Background</h3><div>Previous studies confirmed a link between urinary metals/metalloids and obesity; however, the majority of these studies defined obesity using body mass index (BMI) or waist circumference (WC), and their results were not constantly consistent. Studies defining obesity based on body fat percentage (BFP) is less reported.</div></div><div><h3>Methods</h3><div>A total of 5405 participants aged 35–74 from Guangxi Zhuang ethnic group in China were included in the analysis. Inductively coupled plasma mass spectrometry (ICP-MS) was used to detect the concentrations of 22 metals/metalloids in urine. Using a binary logistic regression model, the impact of individual metal/metalloid on the risk of BFP/obesity was analyzed, and the LASSO regression model was employed to choose metals/metalloids independently related with BFP/obesity to construct a multiple-metal models. The quantile g-computation model was used to evaluate the combined impacts of metals/metalloids on BFP/obesity.</div></div><div><h3>Results</h3><div>In multiple-metal models, compared with the lowest quartile, the highest quartile of urinary concentrations of Mg, Cd, and Ti was significantly associated with a reduced risk of BFP/obesity (Mg: OR=0.66, 95 %CI: 0.51, 0.85; Cd: OR=0.63, 95 %CI: 0.49, 0.82; Ti: OR=0.73, 95 %CI: 0.57, 0.93). Conversely, the highest quartiles of urinary concentrations of Zn, V, and Sb was significantly associated with an increased risk of BFP/obesity (Zn: OR=1.75, 95 %CI: 1.39, 2.22; V: OR=1.63, 95 %CI: 1.25, 2.14; Sb: OR=1.38, 95 %CI: 1.06, 1.79). In quantile g-computation analysis, Mg, Cd, and Sn were the main contributors to negative effects, while Zn, V, and Sb were the main contributors to positive effect, although no significant relationship was observed between the multiple metal/metalloid mixtures and BFP/obesity.</div></div><div><h3>Conclusions</h3><div>According to our study, urinary Mg, Cd, and Ti levels were negatively associated with BFP/obesity risk, and Zn, V, and Sb levels were positively associated with BFP/obesity risk. However, these associations need to be further verified by longitudinal studies, and the molecular mechanisms need to be further explored by animal and cell experiments.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127538"},"PeriodicalIF":3.6,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of zirconia-based oxide on endothelial cell dynamics and extracellular matrix remodeling","authors":"Beatriz de Almeida Camargo ,&nbsp;Geórgia da Silva Feltran ,&nbsp;Célio Junior da Costa Fernandes ,&nbsp;Maria Gabriela Carra ,&nbsp;Margarida Juri Saeki ,&nbsp;Willian F. Zambuzzi","doi":"10.1016/j.jtemb.2024.127537","DOIUrl":"10.1016/j.jtemb.2024.127537","url":null,"abstract":"<div><h3>Introduction</h3><div>Zirconia (ZrO₂) is highly regarded in dental restoration due to its aesthetic compatibility and mechanical properties that align with biological tissues. This study explores the effects of stabilized ZrO2 on endothelial cell function and extracellular matrix (ECM) remodeling, processes critical to successful osseointegration in dental implants.</div></div><div><h3>Methodology</h3><div>Human Umbilical Vein Endothelial Cells (HUVECs) were cultured in ZrO₂ -enriched medium under both static and shear stress conditions. Newly implemented techniques, including detailed zirconia surface characterization using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD), were used to verify material properties. Gene and protein expression related to cell adhesion, proliferation, and ECM remodeling were assessed through RT-qPCR and Western blotting. Zymography was used to evaluate the activity of matrix metalloproteinases (MMP2 and MMP9) involved in ECM remodeling.</div></div><div><h3>Results</h3><div>Characterization data confirmed the stability and structural properties of ZrO₂, revealing a tetragonal crystalline structure and rough surface morphology conducive to cell adhesion. ZrO₂ exposure led to the downregulation of Src, a key regulator of cell adhesion, while upregulating cell cycle regulators p15, CDK2, and CDK4, indicating enhanced cell proliferation. Under shear stress, ZrO₂ modulated TGF-β and MAPK signaling, affecting cell proliferation and angiogenesis. MMP2 and MMP9 activity increased in static conditions but decreased under shear stress, suggesting ZrO₂ dynamic role in ECM remodeling.</div></div><div><h3>Conclusion</h3><div>This study shows that stabilized zirconia (ZrO₂) modulates endothelial cell dynamics and ECM remodeling, key for osseointegration. ZrO₂ downregulated Src expression and upregulated cell cycle regulators, enhancing endothelial proliferation. It also affected TGF-β and MAPK pathways, influencing angiogenesis, and differentially modulated MMP2 and MMP9 activity depending on mechanical conditions. These findings highlight ZrO₂ has potential ability to enhance vascular and tissue integration in dental applications.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127537"},"PeriodicalIF":3.6,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High levels of the health-relevant antioxidant selenoneine identified in the edible mushroom Boletus edulis","authors":"Franziska Peer,&nbsp;Doris Kuehnelt","doi":"10.1016/j.jtemb.2024.127536","DOIUrl":"10.1016/j.jtemb.2024.127536","url":null,"abstract":"<div><h3>Background</h3><div>Selenoneine, the selenium analogue of the sulfur antioxidant ergothioneine, has been ascribed a multitude of beneficial health effects. Natural nutritional sources for this selenium species are, hence, of high interest. So far marine fish is the only significant selenoneine source consumed by larger parts of the population worldwide.</div></div><div><h3>Methods</h3><div>As selenoneine and ergothioneine share their biosynthetic pathways and transport mechanisms and the popular edible porcini mushroom <em>Boletus edulis</em> is rich in ergothioneine and selenium, we conducted a preliminary study investigating a composite sample of two specimens of <em>B. edulis</em> for their selenoneine content by HPLC coupled to elemental and molecular mass spectrometry after aqueous extraction.</div></div><div><h3>Results</h3><div>Selenium speciation analysis by HPLC-ICPMS revealed that ca. 860 µg Se kg⁻¹ wet mass (81 % of the total Se) co-eluted with a selenoneine standard and a minor selenium species with Se-methylselenoneine. The presence of selenoneine was rigorously proven by HPLC-ESI-Orbitrap MS. The selenoneine content of the investigated specimens of <em>B. edulis</em> was higher than that of commonly consumed muscle of marine fish species, like tuna or mackerel.</div></div><div><h3>Conclusion</h3><div>This is the first report of a terrestrial food source containing significant selenoneine levels. Our results suggest that <em>B. edulis</em> might represent a complementary natural supply with this health-relevant selenium species for humans.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127536"},"PeriodicalIF":3.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001561/pdfft?md5=ab6406e2c5511e6c76c56fed2f8b5c43&pid=1-s2.0-S0946672X24001561-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intake and excretion of cadmium of Japanese adult","authors":"Jun Yoshinaga,&nbsp;Minori Ogawa","doi":"10.1016/j.jtemb.2024.127535","DOIUrl":"10.1016/j.jtemb.2024.127535","url":null,"abstract":"<div><h3>Objective</h3><div>It is the aim of this study if the recent Cd intake and excretion is consistent with the regression equation proposed by Ikeda et al. (<em>Environ. Health Prev. Med.</em> 20 (2015) 455–459) which utilized intake/excretion data of 3 decades ago</div></div><div><h3>Method</h3><div>Matched duplicate diet and spot urine samples were collected in 2017–2019 from 150 Japanese adults (85 females and 65 males, mean age being 44.9) for the estimation of daily Cd intake and urinary excretion. Cd concentration in diet and urine was measured by ICP mass spectrometry.</div></div><div><h3>Result</h3><div>Geometric mean (geometric standard deviation) of Cd intake was 12.1 (1.67) μg/person/day or 0.206 (1.66) μg/kg body wt./day. Urinary Cd concentration was 0.54 (2.6) μg/g creatinine or 0.67 μg/L (2.3) (gravimetric correction). These were lower than those reported previously for Japanese populations. Urinary concentration was significantly higher in females than in males for both creatinine and gravimetric corrections. Dietary intake was higher in males than in females but this difference diminished after body weight correction. There was no significant correlation between Cd intake and urinary concentration on individual basis, which was expected because urinary Cd concentration reflects Cd body burden but not recent intake level. Meanwhile, the geometric mean intake and urine concentration was consistent with the regression equation proposed by Ikeda et al., which was based on intake and excretion surveys for 30 non-exposed Japanese female populations.</div></div><div><h3>Conclusion</h3><div>This result supported that the regression equation of Ikeda et al. could be used for conversion of urinary Cd excretion level of a population to intake level.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127535"},"PeriodicalIF":3.6,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel copper complex inhibits the proteasome in skin squamous cell carcinoma induced by DMBA in mice","authors":"Ouadie Mohamed El Yaagoubi ,&nbsp;Larbi Oularbi ,&nbsp;Ouissal Salhi ,&nbsp;Hamid Samaki ,&nbsp;Mama El Rhazi ,&nbsp;Souad Aboudkhil","doi":"10.1016/j.jtemb.2024.127533","DOIUrl":"10.1016/j.jtemb.2024.127533","url":null,"abstract":"<div><div>The proteasomal system is becoming a target for the treatment of several diseases, especially in cancer therapy. The present study aims to develop a novel copper complex that inhibits the proteasome in skin squamous cell carcinoma. New molecules based on the copper complex were synthesized for the first time to assess their potential as proteasome inhibitors, specifically targeting squamous cell carcinoma induced by 7,12-dimethylbenz(a)anthracene (DMBA) in mouse models. Fourier Transform Infrared (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), and energy dispersive X-ray analysis (EDX) were carried out to characterize this new copper complex. Notably, the presence of a papilloma (skin tumor) was confirmed by histopathological analysis. Subsequent investigation included the quantification of proteasome levels using a sandwich ELISA test, and the catalytic activity of the 20S proteasome was determined by measuring the fluorescence emitted after the cleavage of 7-amino-4-methylcoumarin (AMC). Hence, X-ray crystallography indicates that all Cu atoms are five-coordinated in a square-pyramidal configuration and biological activity of copper Schiff base complex, which exhibits high proteasome inhibitory activities with particular selectivity of <em>β</em>5 subunit. The pharmacokinetic properties (ADMET) of the copper complex named Cu(L1) showed encouraging results with very low toxicity, distribution, and absorption. Structure–activity relationship (SAR) information obtained from Cu(L1) demonstrated its selectivity and potent inhibition for <em>β</em>5 subunit. In this regard, this copper complex has emerged as a novel therapy for skin cancer.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127533"},"PeriodicalIF":3.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The concentrations of microelements in forest mushrooms are influenced by soil pH and C/N ratio and less by stand characteristics","authors":"Katarzyna Stojek ,&nbsp;Mirosław Krośniak ,&nbsp;Barbara Bobrowska-Korczak ,&nbsp;Patryk Czortek ,&nbsp;Lucas Decock ,&nbsp;Michael Scherer-Lorenzen ,&nbsp;Annemieke Verbeken ,&nbsp;Kris Verheyen ,&nbsp;Bogdan Jaroszewicz","doi":"10.1016/j.jtemb.2024.127534","DOIUrl":"10.1016/j.jtemb.2024.127534","url":null,"abstract":"<div><p>The fruitbodies or sporocarps formed by mushrooms can accumulate mineral elements, such as selenium, zinc or copper, making them an important source of micronutrients essential to humans. However, the literature about environmental factors affecting mineral composition in mushrooms is scarce and limited to the ambiguous impact of soil properties and region. In our study, we investigated the effects of tree stand characteristics (tree species and tree canopy cover), understory cover, and soil properties (pH and C/N ratio of the soil) on the concentration of minerals in six edible mushroom species: <em>Laccaria laccata</em>, <em>L. proxima</em>, <em>L. amethystina</em>, <em>Lepista nuda</em>, <em>Lycoperdon perlatum</em>, and <em>Calvatia excipuliformis</em>, collected on 20 plots covered by stands of different tree species composition and varying in the understory cover. We estimated the concentration of eight elements (Zn, Se, Mg, Mn, Cu, Co, Cr, Mo) using the ICP-MS (Inductively Coupled Plasma - Mass Spectrometry) technique and compared their concentration between the plots, using ordination and linear regression methods. Our research revealed that mushroom species identity, including its ecological role and phylogenetic affinity, had the greatest effect on the mineral composition of mushrooms. The effect of environmental factors depended also on the micronutrient identity. Some elements were not affected at all (Co, Cr), some only by soil reaction or C/N ratio (Mn, Mg, Cu), while others were influenced by both tree stand characteristics and soil properties (Se, Zn, Mo). This knowledge enables us to maximize the content of minerals in harvested mushrooms by collecting them in specific areas. For example, mushrooms, which are sources of Se and Zn, can be gathered in coniferous forests characterized by acid soils, low canopy cover, and minimal understory cover. This targeted collection approach can effectively increase the mineral content in harvested mushrooms, thereby enhancing their health benefits.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127534"},"PeriodicalIF":3.6,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001548/pdfft?md5=2237eea8e979b4ad16e8a956cab4ba76&pid=1-s2.0-S0946672X24001548-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142272805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in breast cancer therapy: The promise of copper nanoparticles","authors":"Chandana Surya ,&nbsp;Ashoka Babu Vechham Lakshminarayana ,&nbsp;Sameera Hammigi Ramesh ,&nbsp;Selvaraj Kunjiappan ,&nbsp;Panneerselvam Theivendren ,&nbsp;A. Santhana Krishna Kumar ,&nbsp;Damodar Nayak Ammunje ,&nbsp;Parasuraman Pavadai","doi":"10.1016/j.jtemb.2024.127526","DOIUrl":"10.1016/j.jtemb.2024.127526","url":null,"abstract":"<div><h3>Background</h3><p>Breast cancer (BC) is the most prevalent cancer among women worldwide and poses significant treatment challenges. Traditional therapies often lead to adverse side effects and resistance, necessitating innovative approaches for effective management.</p></div><div><h3>Objective</h3><p>This review aims to explore the potential of copper nanoparticles (CuNPs) in enhancing breast cancer therapy through targeted drug delivery, improved imaging, and their antiangiogenic properties.</p></div><div><h3>Methods</h3><p>The review synthesizes existing literature on the efficacy of CuNPs in breast cancer treatment, addressing common challenges in nanotechnology, such as nanoparticle toxicity, scalability, and regulatory hurdles. It proposes a novel hybrid method that combines CuNPs with existing therapeutic modalities to optimize treatment outcomes.</p></div><div><h3>Results</h3><p>CuNPs demonstrate the ability to selectively target cancer cells while sparing healthy tissues, leading to improved therapeutic efficacy. Their unique physicochemical properties facilitate efficient biodistribution and enhanced imaging capabilities. Additionally, CuNPs exhibit antiangiogenic activity, which can inhibit tumor growth by preventing the formation of new blood vessels.</p></div><div><h3>Conclusion</h3><p>The findings suggest that CuNPs represent a promising avenue for advancing breast cancer treatment. By addressing the limitations of current therapies and proposing innovative solutions, this review contributes valuable insights into the future of nanotechnology in oncology.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127526"},"PeriodicalIF":3.6,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of heavy metal levels in cow's milk and associated health risks in the vicinity of the MIDROC Laga Dambi gold mine in Ethiopia","authors":"Workineh Mengesha Fereja ,&nbsp;Chuluke Muda ,&nbsp;Abraham Alemayehu Labena","doi":"10.1016/j.jtemb.2024.127529","DOIUrl":"10.1016/j.jtemb.2024.127529","url":null,"abstract":"<div><h3>Introduction</h3><p>The possible health effects of consuming milk contaminated with heavy metals have been the subject of considerable concern worldwide.</p></div><div><h3>Objective</h3><p>The aim of this study was to determine the level of heavy metals in cow's milk in the vicinity of MIDROC Laga Dambi gold mine and to assess their possible health risks for consumers.</p></div><div><h3>Methods</h3><p>Nine composite samples were formed by aggregating 243 milk samples obtained in triplicates from 81 domestic milk-producing households. Inductively coupled plasma-optical emission spectroscopy was used to measure the amount of heavy metals after samples digestion under optimal conditions.</p></div><div><h3>Results</h3><p>The heavy metals concentrations obtained were 13.913˗7.843, 9.505˗3.589, 5.972˗3.147, 2.288˗1.851, 0.403˗0.143, 0.436˗0.128, 0.26˗0.153, 0.143˗0.048, 0.160˗ND (not detected), and 0.140˗ND mgkg⁻¹for Fe, Zn, Pb, Mn, Hg, Cr, Cd, As, Ni, and Co, respectively. Of the heavy metals identified, the levels of Pb, As, Cd, and Hg exceeded the recommended value. Based on the estimated daily intake (EDI), the total health quotient (THQ) is higher than unity even for Pb alone. It has been found that the consumption of cow milk increases the health index (HI) by 2.972. Ninety five percent of the HI in the study area was explained by the toxic heavy metals (Pb, Cd, As, and Hg) in the cow milk, which were found to be beyond the safe limit.</p></div><div><h3>Conclusion</h3><p>This demonstrates that there is a health risk to the population who consume cow’s milk sourced from the vicinity of MIDROC Laga Dambi gold mine. To safeguard the public’s health, we advised strict monitoring and legislative control for the safety of cow’s milk originating from study area.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127529"},"PeriodicalIF":3.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper excess in psychiatric disorders: a focus on mood spectrum disorders and sex","authors":"Rosanna Squitti ,&nbsp;Mauro Rongioletti ,&nbsp;Silvia Fostinelli ,&nbsp;Altea Severino ,&nbsp;Cristian Bonvicini ,&nbsp;Andrea Geviti ,&nbsp;Alessandra Martinelli ,&nbsp;Giovanni Battista Tura ,&nbsp;Roberta Ghidoni","doi":"10.1016/j.jtemb.2024.127532","DOIUrl":"10.1016/j.jtemb.2024.127532","url":null,"abstract":"<div><h3>Background</h3><p>Meta-analyses show increased copper (Cu) levels in major depression disorder. However, the association of Cu biomarkers with clinical classification in other mental health disorders has not been fully explored.</p></div><div><h3>Methods</h3><p>To this aim, we compared an extensive panel of Cu biomarkers, composed of Cu, ceruloplasmin (Cp) Cp activity, Cp specific activity, Cu not bound to ceruloplasmin (non-Cp Cu, also known as ‘free’ copper) in 171 consecutive patients affected by psychiatric disorders and in 61 healthy controls (HC) using MANOVA adjusting for the effect of sex and age, and studied their association with the clinical scale outcomes at psychiatric examination, namely Global Assessment of Functioning, Clinical Global Impression, and Brief Psychiatric Rating Scale.</p></div><div><h3>Results</h3><p>individuals with psychiatric disorders were classified as 109 patients affected by mood spectrum disorders (MSD), 20 patients with schizophrenia spectrum disorders (SSD), and 42 with personality disorders (PD). Cu and non-Cp Cu were increased in psychiatric individuals than in HC, which also differed among the patients stratified per the clinical classification, being higher in the MSD individuals. The analysis stratified for sex revealed that women from the patient group, and specifically from the MSD group, had increased levels of Cu and non-Cp Cu than healthy women, while no difference was revealed in men. A logistic regression model considering the effect of sex and age revealed that non-Cp Cu could explain 26 % increased odds of having MSD per µmol/L unit increase (OR = 1.26; p = 0.0008; 95 % CI 1.099–1.436), that reached 40 % when considering only women. This result was driven by non-Cp Cu that correctly classified 64.1 % MSD (70 % in women) individuals vs. HC in a decision tree model, with values higher than 2.1 µmol/L which could distinguish the majority of MSD patients (86.3 % MSD vs. 13.7 % HC in women). None of the biological variables under study correlated with outcomes of the clinical scales, substances, or alcohol abuse.</p></div><div><h3>Conclusion</h3><p>Current results suggest mild Cu toxicity in women with MSD, as revealed by a value of non-Cp Cu higher than 2.1 µmol/L, which can be further investigated to assess its potential diagnostic accuracy in bigger and longitudinal cohorts.</p></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127532"},"PeriodicalIF":3.6,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0946672X24001524/pdfft?md5=e008593a0ba1378aa5ff35527a511a9c&pid=1-s2.0-S0946672X24001524-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142243977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}