Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Determinants of iron, zinc, and copper concentrations in the hair of dogs and cats","authors":"Emanuela Badea ,&nbsp;Gheorghe Valentin Goran ,&nbsp;Cristina Ţoca","doi":"10.1016/j.jtemb.2026.127826","DOIUrl":"10.1016/j.jtemb.2026.127826","url":null,"abstract":"<div><div>Trace elements such as iron (Fe), copper (Cu), and zinc (Zn) are essential dietary components that support a broad range of biochemical and physiological functions. Despite being required in trace amounts, disturbances in their homeostasis can lead to a wide range of health issues. Their assessment in biological matrices, including hair, in companion animals continues to be explored as a means of understanding nutritional status, environmental exposure, and pathological conditions. However, data for companion animals remain limited. This study evaluated the influence of selected individual (sex, age) and environmental (diet type, living environment) factors on Fe, Zn, and Cu concentrations in the hair of clinically healthy dogs and cats living in Bucharest, Romania. Elemental quantification was performed using ICP-MS following acid digestion. Median concentrations were compared using Mann–Whitney U and Kruskal–Wallis tests, and associations among elements were evaluated using Spearman’s rank correlation with Benjamini–Hochberg correction (FDR 0.05) to control for false discoveries. Dogs exhibited significantly higher hair Fe (168.29 µg/g) and Cu (6.99 µg/g) concentrations than cats (57.67 µg/g Fe; 5.86 µg/g Cu), whereas Zn levels were similar between species (93.12 µg/g in dogs; 101.20 µg/g in cats). Sex, age, and diet type showed no significant influence on Fe, Zn, or Cu in either species. In contrast, the living environment significantly affected Fe levels: dogs kept outdoors had markedly higher Fe concentrations (312.21 µg/g) than indoor dogs (78.31 µg/g), and the same pattern was observed in cats (111.05 µg/g in outdoor cats; 40.52 µg/g in indoor cats), while Zn and Cu remained unaffected. All pairwise correlations among Fe, Zn, and Cu were positive and remained statistically significant after FDR correction. Zn–Cu demonstrated the strongest association in both species (ρ = 0.773 in dogs; ρ = 0.883 in cats), with cats showing overall stronger inter-element correlations than dogs. These findings contribute to the limited reference data on companion animal hair mineral profiles and highlight the potential value of hair analysis for exploring environmental influences and trace element interactions in dogs and cats.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"94 ","pages":"Article 127826"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146081679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalization of carbon nano-onions for potential biomedical applications","authors":"Mariana Prodana ,&nbsp;Daniela Ionita ,&nbsp;Calin C. Moise ,&nbsp;Oana A. Lazar ,&nbsp;Marius Enachescu ,&nbsp;Narcisa Mederle","doi":"10.1016/j.jtemb.2026.127822","DOIUrl":"10.1016/j.jtemb.2026.127822","url":null,"abstract":"<div><div>Multi-shell fullerenes, known as carbon nano-onions (CNOs), are one of the allotrope forms of carbon that have received less research efforts compared to other carbon allotropes, e.g., carbon nanotubes, graphene, etc. In this work, we report the synthesis of “<em>in-house made</em>” CNOs, prepared by pulsed laser ablation technique, and we demonstrate the functionalization of CNOs with carboxylic groups followed by cisplatin adsorption, as a hybrid functionalization method. The results involve platinum-oxidizing nano-onions for further applications in the biomedical field, knowing that functionalization of CNOs with different functional groups improves their solubility and biocompatibility, resulting in an increased ability to penetrate the cells. The chemical composition, molecular structure, and morphological properties of the prepared samples were characterized by micro-Raman spectroscopy, Attenuated Total Reflection Infrared Transformed Fourier spectroscopy (FTIR), and High-Resolution Scanning Transmission Electron Microscopy (HR-STEM) equipped with the Energy-Dispersive X-ray spectroscopy module. The amount of Pt ion release from the cisplatin functionalized samples was studied using Inductively Coupled Plasma Mass Spectrometry (ICP-MS).</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"94 ","pages":"Article 127822"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin-selenium nanoparticles: A promising approach in disease prevention and treatment","authors":"Sahar Ghoflchi ,&nbsp;Sercan Karav ,&nbsp;Hossein Hosseini ,&nbsp;Amirhossein Sahebkar","doi":"10.1016/j.jtemb.2026.127824","DOIUrl":"10.1016/j.jtemb.2026.127824","url":null,"abstract":"<div><div>Curcumin, the primary active ingredient from turmeric's rhizome, and selenium, a vital human element, have received significant scientific attention in medical research. The combination of curcumin and selenium has been considered an emerging approach to preventing and treating diseases due to their ability to reduce oxidative stress, regulate inflammation, and enhance the immune system. The use of nanotechnology, especially in the form of curcumin-selenium nanoparticles (CurSeNPs), has provided a novel solution to overcome the limitations of the use of curcumin and selenium, such as the low bioavailability of curcumin and the toxicity of selenium at high doses. This review aims to explore the research on the effects of CurSeNPs on various diseases and assess their therapeutic potential. This review aims to evaluate previous studies exploring the effects of Cur–SeNPs on various diseases.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"94 ","pages":"Article 127824"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium perborate tetrahydrate triggers mitochondrial dysfunction in Leishmania infantum parasites and enhances antileishmanial activity in macrophages","authors":"Zeynep Islek ,&nbsp;Hazal Uraz ,&nbsp;Hatice Ilke Ozeken ,&nbsp;Rengin Reis ,&nbsp;Mehmet Hikmet Ucisik ,&nbsp;Ezgi Taskan ,&nbsp;Fikrettin Sahin","doi":"10.1016/j.jtemb.2026.127845","DOIUrl":"10.1016/j.jtemb.2026.127845","url":null,"abstract":"<div><div>Sodium Perborate Tetrahydrate (SPT) is a potent semimetal element possessing intrinsic antimicrobial activities against various microorganisms including protozoan parasites. The crucial role of boron in antimicrobial therapy is associated with both its biocidal properties and the ability to reduce inflammatory processes. High water solubility and bioavailability of the compound took advantage of its medical use. The objective of this study is to evaluate the antileishmanial potential of SPT for Leishmaniasis on <em>Leishmania (L.) infantum</em> parasites and <em>Leishmania</em>-infected RAW264.7 macrophage cell line as well as non-infected macrophages. The antileishmanial mechanism was analyzed through cell viability, mitochondrial membrane potential, measurement of Reactive Oxygen Species (ROS) and glutathione level, cell cycle, and gene expression levels on a molecular basis. Accordingly, SPT treatment with a 50 % inhibitory concentration (IC₅₀) value of 71.99 ± 7.51 µM indicated a significant efficacy on <em>L. infantum parasites</em> without causing any toxicity on healthy macrophages. ≥ 100 µM concentrations of SPT treatment led to a decrease in the mitochondrial membrane potential of <em>L. infantum</em> promastigotes, which was reduced to below 53 % and 35 % at 24 and 48 h, respectively. In parallel, cell cycle arrest at the S and G2/M phases, and apoptotic cell death of 41.52 ± 5.79 % after 150 µM SPT treatment verified the antileishmanial therapy. Moreover, the division of <em>L. infantum</em> parasites within the infected macrophages remained at 73.67 % by ≥ 75 µM SPT concentrations. SPT had no significant effect on intracellular macrophage ROS levels. Metacaspase5 gene expression was consistent with mitochondrial membrane potential data confirming the higher apoptotic-like death at 100 µM SPT treatment. Furthermore, pro-inflammatory IFN-γ levels overexpressed significantly in ≥ 50 µM SPT treatment, whereas only 200 µM SPT upregulated the TNF-α and IL-12(B) inflammation markers. Anti-inflammatory IL-10 and TGF-ß1gene expressions were downregulated in infected macrophages upon treatment with 100 µM SPT. The antileishmanial efficacy of SPT highlights the potential therapy for future in vivo studies.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"94 ","pages":"Article 127845"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146230376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metallic nanoparticles in diabetes mellitus: Mechanistic insights and therapeutic perspectives","authors":"Preeti Pal,&nbsp;Ashutosh Pareek","doi":"10.1016/j.jtemb.2026.127833","DOIUrl":"10.1016/j.jtemb.2026.127833","url":null,"abstract":"<div><div>Diabetes mellitus is a chronic metabolic disorder that affects millions worldwide and continues to rise in prevalence. While conventional therapies such as insulin and oral hypoglycemic drugs provide glycemic control, they often fail to address underlying mechanisms like oxidative stress, inflammation, and β-cell dysfunction that drive disease progression. Metallic nanoparticles (MNPs) have emerged as promising multifunctional agents owing to their tunable physicochemical properties, high surface reactivity, and ability to be functionalized for targeted applications. Recent studies demonstrate that nanoparticles such as silver, zinc oxide, gold, titanium dioxide, copper oxide, and iron oxide can enhance insulin secretion and sensitivity, regulate glucose homeostasis, mitigate oxidative stress, and modulate immune and microbial pathways. Application-focused evidence highlights MNPs utility in drug delivery, glucose sensing, localized wound therapy, β-cell preservation/regeneration, and mitigation of diabetic complications (nephropathy, neuropathy, retinopathy). However, clinical momentum remains strongest for localized products and device prototypes, whereas systemic candidates are constrained by biodistribution/clearance, chronic safety requirements, and CMC/GMP reproducibility. Green-synthesized MNPs further offer eco-friendly and biocompatible alternatives, showing synergistic effects with phytochemicals. Despite these advances, translation to clinical practice is still in its infancy due to limited human trials, incomplete pharmacokinetic data, and safety concerns. This review consolidates current mechanistic insights, therapeutic applications, and clinical progress of MNPs in diabetes management, also highlights future research directions for a more translationally disciplined approach. By bridging laboratory research with translational strategies, MNPs hold strong potential to transform the therapeutic landscape of diabetes and its complications.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"94 ","pages":"Article 127833"},"PeriodicalIF":3.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146121473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of newly synthesized boron compounds (BPM and BPD) on Mycobacterium tuberculosis and A549 cells","authors":"Elif AYDIN ,&nbsp;Melda BOLAT ,&nbsp;Meliha KOLDEMIR GUNDUZ ,&nbsp;Dursun Ali KOSE","doi":"10.1016/j.jtemb.2025.127809","DOIUrl":"10.1016/j.jtemb.2025.127809","url":null,"abstract":"<div><div>In this study, the cytotoxic and antimycobacterial effects of two newly synthesized boron-based compounds, boron phenylalanine monoester (BPM) and boron phenylalanine diester (BPD), were investigated in human lung adenocarcinoma cells (A549) and <em>Mycobacterium tuberculosis</em> H37Rv strain. MTT assays revealed dose-dependent antiproliferative activity, with IC₅₀ values of 1.5 mM for BPM and 1.16 mM for BPD, indicating greater potency of BPD at lower concentrations. To contextualize potency, we report MICs in both mM and µg/mL. While the current MICs are higher than typical literature benchmarks for front-line comparators, these chemotypes provide a starting point for optimization. Gene expression analysis after 48-hour treatment showed significant upregulation of TNF-α, p53, and Bax, and downregulation of IL-6 and Bcl-2 (p &lt; 0.05). The increase in TNF-α expression (p = 0.0159 for BPM; p = 0.0095 for BPD) suggests immune pathway activation, while the rise in p53 levels supports apoptotic induction. In antimicrobial assays, BPD demonstrated superior efficacy with a MIC value of 25 mM, compared to 50 mM for BPM. Collectively, these findings indicate that BPM and especially BPD show preliminary activity that warrants further optimization for dual anticancer and antimycobacterial applications.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"93 ","pages":"Article 127809"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145791275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZIP3 is essential for the encephalitogenic function of pTh17 cells by regulating intracellular zinc levels","authors":"Dominik Drmić ,&nbsp;Liwei Wang ,&nbsp;Lucile Noyer ,&nbsp;Li Zhong ,&nbsp;Stefan Feske","doi":"10.1016/j.jtemb.2026.127819","DOIUrl":"10.1016/j.jtemb.2026.127819","url":null,"abstract":"<div><h3>Background</h3><div>Zinc (Zn²⁺) is the second most abundant trace metal in eukaryotes and essential for many cellular functions. It possesses well-established immunomodulatory properties and disruption of Zn²⁺ signaling compromises immune functions resulting in immunodeficiency. In addition, Zn²⁺ has been implicated in several autoimmune diseases such as multiple sclerosis (MS).</div></div><div><h3>Methods</h3><div>We used a forward genomic screen to identify Zn²⁺ transporters that control the function of encephalitogenic T helper (Th) 17 cells, and thus, drive CNS inflammation in the experimental autoimmune encephalomyelitis (EAE) mouse model of MS. We measured cytosolic Zn²⁺ levels in T cells with a genetically encoded Zn²⁺ reporter and used confocal microscopy to determine the subcellular localization of ZIP3. We used shRNA and CRISPR gene editing to delete <em>Slc39a3</em> (encoding ZIP3) to determine its role in CD4⁺ T cells <em>in vitro</em> and pathogenic Th17 (pTh17) cells <em>in vivo</em> in EAE.</div></div><div><h3>Results</h3><div>Using a shRNA screen in myelin-specific pTh17 cells <em>in vivo</em>, we found that ZIP3 is required for their expansion in the CNS of mice with EAE. When ectopically expressed, ZIP3 was located predominantly in the Golgi apparatus. Deletion of <em>Slc39a3</em> resulted in decreased cytosolic Zn²⁺ levels selectively in pTh17 cells without affecting those in non-polarized CD4⁺ T cells. <em>Slc39a3-</em>deficient pTh17 cells showed an increased susceptibility to apoptotic cell death and reduced CD69 expression <em>in vitro</em>, while proliferation and cytokine production remained intact. <em>Slc39a3</em> deletion in pTh17 cells significantly reduced the severity of EAE by reducing leukocyte infiltration of the CNS.</div></div><div><h3>Conclusions</h3><div>ZIP3 is essential for the encephalitogenic function of pTh17 cells and promoting EAE, likely by regulating cytosolic Zn²⁺ levels.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"93 ","pages":"Article 127819"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145976850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aluminium adjuvants and childhood health: a call for science","authors":"Guillemette Crépeaux ,&nbsp;Jeremy R. Hammond ,&nbsp;Jonathan B. Handley ,&nbsp;Brian Hooker ,&nbsp;Karl Jablonowski ,&nbsp;Lluís Luján ,&nbsp;James Lyons-Weiler ,&nbsp;Marika Nosten-Bertrand ,&nbsp;Christopher A. Shaw ,&nbsp;Yehuda Shoenfeld ,&nbsp;Lucija Tomljenovic ,&nbsp;Christopher Exley","doi":"10.1016/j.jtemb.2025.127810","DOIUrl":"10.1016/j.jtemb.2025.127810","url":null,"abstract":"<div><div>In July 2025, Andersson et al. reported in Annals of Internal Medicine that early-life exposure to Aluminium (Al)-adjuvanted vaccines was not associated with increased risk of 50 chronic diseases, based on a Danish cohort of 1.2 million children. While widely cited as reassuring evidence of Al-Based Adjuvant (ABA) safety, closer scrutiny reveals major methodological and conceptual flaws. Specifically, the study demonstrates limited understanding of Al toxicology, weaknesses in cohort design and statistical analysis, and insufficient transparency regarding potential conflicts of interest. We argue that these shortcomings prevent meaningful conclusions about ABA safety, particularly in relation to neurodevelopmental and autoimmune outcomes, and highlight the need for more rigorous, transparent, and scientifically grounded investigations.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"93 ","pages":"Article 127810"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145867121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-ray fluorescence analysis of elemental concentrations in human tongue tissues: Distinguishing healthy from carcinoma profiles","authors":"Luísa Zagalo ,&nbsp;Luísa L. Gonçalves ,&nbsp;Carlos Zagalo ,&nbsp;Maria João Oliveira ,&nbsp;Pedro Oliveira ,&nbsp;José A.A. Brito","doi":"10.1016/j.jtemb.2025.127811","DOIUrl":"10.1016/j.jtemb.2025.127811","url":null,"abstract":"<div><div>This study explores the feasibility of Wavelength Dispersive X-ray Fluorescence (WDXRF) to distinguish between healthy and carcinoma tongue tissues based on elemental concentration profiles. The concentrations of six chemical elements (phosphorus, sulphur, calcium, iron, zinc and copper) were determined in formalin-fixed and paraffin-embedded tissue samples from both tumour regions and histologically validated healthy margins in 31 patients with tongue squamous cell carcinoma. Mean concentrations for every sample were calculated from three replicate measurements and analysed using discriminant analysis and mixed linear models. Although the discriminant model lacked statistical relevance, the structural matrix suggested iron, copper and zinc contributed the most to the differentiation between tissue types. These elements were subsequently investigated further by using mixed linear models, which confirmed they had significantly reduced concentrations in carcinoma tissues (p &lt; 0.001), having maintained significant results after Bonferroni correction. Furthermore, a decreased iron concentration in carcinoma tissue of patients who reported frequent alcohol consumption was observed (p = 0.003), while tobacco use showed no significant effect on the element’s concentrations. Although exploratory, our results show changes in specific elemental concentrations, something that may help define oral cancer and support the incorporation of X-ray fluorescence into complementary diagnostic means.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"93 ","pages":"Article 127811"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145840523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thallium accumulation and transfer through developmental stages of the common green bottle fly, Lucilia sericata, exposed to Tl(I) and Tl(III) species","authors":"Julita Malejko ,&nbsp;Krzysztof Deoniziak ,&nbsp;Kinga Sulej ,&nbsp;Justyna Adamska ,&nbsp;Beata Godlewska-Żyłkiewicz","doi":"10.1016/j.jtemb.2025.127812","DOIUrl":"10.1016/j.jtemb.2025.127812","url":null,"abstract":"<div><h3>Background</h3><div>Thallium accumulation in insects, which is relevant to both environmental and forensic toxicology, is poorly understood. Thallium toxicity is related to its chemical speciation. <em>Objective:</em> Study of thallium bioaccumulation by the green bottle fly <em>Lucilia sericata</em> exposed to different thallium compounds (TlCH₃COO and Tl(CH₃COO)₃) and doses. <em>Method:</em> The content of thallium, potassium, and sodium in feeding substrate, larvae, puparial cases, and adult flies was determined by inductively coupled plasma mass spectrometry (ICP-MS). <em>Results:</em> The bioaccumulation factors (BAFs) of Tl(I) remained stable across doses (0.28–0.30 for larvae, 0.10–0.13 for adults and 0.4–0.5 for puparial cases), while BAFs of Tl(III) decreased with increasing dose from 0.38 to 0.27 in larvae and from 0.15 to 0.07 in adults but increased with increasing dose from 0.5 to 0.8 in puparial cases. The 46–49 % of Tl(I) and 50–94 % of Tl(III) accumulated in larvae were eliminated by deposition in puparial cases. Larval weight and mortality were unaffected by the presence of Tl(I) or Tl(III) in the feeding substrate at the doses tested. <em>Conclusion:</em> Thallium accumulation in the life stages of <em>L. sericata</em> depends on its chemical form (Tl(I) and Tl(III)) and content in the insect diet. Its main elimination route is the deposition in puparial cases. Potassium naturally present in the feed may mitigate Tl toxicity to the larvae. Our study demonstrates the usefulness of <em>L. sericata</em> as a reliable model organism for monitoring environmental thallium pollution and thallium poisoning in forensic entomotoxicological investigations.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"93 ","pages":"Article 127812"},"PeriodicalIF":3.6,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145867090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}