Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Effect of blueberries on non-heme iron absorption in adult women","authors":"Gabriela Galán-Ramírez ,&nbsp;Daniela Moya ,&nbsp;Manuel Olivares ,&nbsp;Lee A. Meisel ,&nbsp;Miguel Arredondo ,&nbsp;Fernando Pizarro","doi":"10.1016/j.jtemb.2025.127753","DOIUrl":"10.1016/j.jtemb.2025.127753","url":null,"abstract":"<div><div>Iron deficiency, the leading cause of anemia, can be exacerbated by dietary inhibitors such as polyphenols in fruits and vegetables. Despite their high polyphenol content, blueberries contain antioxidant compounds such as ascorbic acid and carotenoids, which are known to enhance iron absorption. This study aims to assess the net effect of blueberry consumption on non-heme iron absorption in healthy, non-menopausal women. Fourteen women aged 38–50 years, with contraception and a negative pregnancy test, participated in the study. After overnight fasting, the subjects were administered iron with or without fresh blueberries on four different intervention days. On day 1, the subjects received FeSO₄ solution as control (C); on day 2, FeSO₄ + 100 g of blueberry (B); on day 14, FeSO₄ + 100 g of blueberry + standardized breakfast (A); and on day 15, FeSO_{4 +} breakfast (D). All servings contained 5 mg of Fe labeled with radioactive isotopes (⁵⁵Fe, ⁵⁹Fe). Blood samples were taken on days 14 and 28 to measure the iron bioavailability and nutritional status. The iron bioavailability in the different groups was (geometric mean and range ±1 SD): C= 30.2 % (13.8–66.0 %), B= 6.8 % (2.1–21.7 %), A= 7.5 % (2.3–24.0 %), and D= 5.7 % (1.5–22.4 %); ANOVA for repeated measures F= 10.2 p &lt; 0.0001 (Dunnett post hoc test: C vs. A, B, and D, p &lt; 0.0001). In conclusion, this study found that fresh blueberries, with or without breakfast, significantly decreased non-heme iron absorption. Given the small sample size and the limited population studied, these findings should be considered preliminary and warrant confirmation in larger studies.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127753"},"PeriodicalIF":3.6,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heavy metals and trace elements in a threatened population of guanay cormorants (Leucocarbo bougainvilliorum) from an industrialized bay in the Humboldt Current System, Chile","authors":"Jorge Carretero ,&nbsp;Ana M. García-Cegarra ,&nbsp;Emma Martínez-López","doi":"10.1016/j.jtemb.2025.127749","DOIUrl":"10.1016/j.jtemb.2025.127749","url":null,"abstract":"<div><div>Metal contamination from industrial and mining activities threatens coastal and marine ecosystems, leading to bioaccumulation in marine organisms and biomagnification through the trophic chain. The Guanay cormorant (<em>Leucocarbo bougainvilliorum</em>), an endemic species of the Humboldt Current System, is experiencing population declines and serves as a key bioindicator of heavy metal contamination due to its high trophic position, reliance on commercially exploited pelagic fish, and capacity to accumulate contaminants over time. This study analyzed the concentrations of five heavy metals (Hg, As, Pb, Cr, Cd) and three trace elements (Cu, Zn, Se) in liver, muscle, feathers, and kidney of 10 Guanay cormorants found dead in Mejillones Bay, Chile, a highly industrialized area. Feathers exhibited the highest concentrations of Cr (2.03 ± 0.63 mg/kg), Cu (19.72 ± 3.82 mg/kg), and Zn (109.84 ± 11.95 mg/kg), emphasizing their role in detoxification. Females showed significantly higher Cu and Pb concentrations in muscle and Cr in kidneys, likely due to physiological and hormonal differences related to reproduction. Correlations between metal concentrations across tissues suggest shared bioaccumulation mechanisms, while the Hg:Se molar ratio (e.g., 0.25 ± 0.04 in liver) indicated a protective role of Se against Hg toxicity. Metal concentrations were comparable to those reported in other marine cormorants but differed from freshwater populations, highlighting the influence of habitat on metal accumulation. Although current levels do not indicate acute toxicity, the ongoing population decline of Guanay cormorants underscores the need for long-term biomonitoring. Future research incorporating biochemical and enzymatic biomarkers is essential to assess potential sublethal effects and long-term ecological risks associated with heavy metal exposure in this species.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127749"},"PeriodicalIF":3.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc and circulating microRNAs in women with insulin resistance","authors":"Danielle Caroline da Silva Dias ,&nbsp;Graziela Biude Silva Duarte ,&nbsp;Leonam da Silva Pereira Batista ,&nbsp;Marcelo Macedo Rogero ,&nbsp;Fernando Barbosa Jr. ,&nbsp;Silvia Maria Franciscato Cozzolino ,&nbsp;Raquel Costa Silva Dantas-Komatsu ,&nbsp;Karina Zaira Silva Marinho Costa ,&nbsp;Bruna Zavarize Reis","doi":"10.1016/j.jtemb.2025.127748","DOIUrl":"10.1016/j.jtemb.2025.127748","url":null,"abstract":"<div><h3>Introduction</h3><div>Insulin resistance (IR) is a key factor in the development of type 2 diabetes and is potentially associated with zinc deficiency due to the role of this mineral in the metabolism of this hormone. MicroRNAs (miRs) can regulate insulin signaling pathway, glucose metabolism, and inflammation, and their expression can be modulated by different nutrients and bioactive compounds. Nutrimiromics investigates how nutrients modulate gene expression through miRs. However, the associations between miRNAs related to IR mechanisms and zinc status in the body remains unclear. Therefore, this study aimed to evaluate the associations of plasma zinc concentration with the expression of miR-191–5p, miR-188–5p, miR-145–5p, and miR-143–3p in overweight and IR women<strong>.</strong></div></div><div><h3>Materials and methods</h3><div>This comparative cross-sectional study included 50 overweight or obese women, aged between 18 and 60 years. IR classification followed Stern's criteria. Circulating miR expression, plasma zinc concentration, biochemical profile, inflammatory biomarkers, and anthropometric parameters were evaluated.</div></div><div><h3>Results</h3><div>Six percent of the participants had plasma zinc concentrations below the reference value (&lt;70 µg/dL). When categorizing plasma zinc values by their median concentration (≤90 µg/dL and &gt;90 µg/dL), a significant difference was observed in the fibrinogen concentration (p = 0.016) and the expression of three miR evaluated (miR-191–5p, miR-145–5p, and miR-143–3p). A significant inverse correlation was found between plasma zinc concentration and miR-145–5p (p = 0.006), miR-143–3p (p = 0.011), miR-191–5p (p = 0.002), and the serum fibrinogen concentration (p = 0.038). Significant inverse correlations were observed between insulin and miR-191–5p (p = 0.007), miR-188–5p (p = 0.029), miR-145–5p (p = 0.015), and miR-143–3p (p = 0.001). miR-191–5p (p = 0.037) and miR-143–3p (p = 0.021) were inversely correlated with HOMA-IR. The miR-145–5p was negatively correlated with IL-1β (p = 0.006), IL-6 (p = 0.004), and IFN-γ (p = 0.020), and miR-143–3p was negatively correlated with IL-6 (p = 0.018). According to the adjusted logistic regression model, high expression of miR-191–5p (OR=0.06; p = 0.012) was inversely associated with the plasma zinc concentration. In the functional enrichment analysis conducted with the list of target genes, six Gene Ontology (GO) terms were significantly enriched.</div></div><div><h3>Conclusion</h3><div>All four miRs showed an inverse correlation with insulin, indicating that zinc status may influence miR expression, potentially affecting biological processes involved in IR. This study identified significant expression of miR-143–3p, miR-145–5p, and miR-191–5p at plasma zinc concentrations above 90 μg/dL.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127748"},"PeriodicalIF":3.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hexavalent chromium exposure impacts the metabolome of human lung fibroblast cells","authors":"James T.F. Wise ,&nbsp;Davide Chiarugi ,&nbsp;Jinhua Chi ,&nbsp;John Pierce Wise Sr.","doi":"10.1016/j.jtemb.2025.127750","DOIUrl":"10.1016/j.jtemb.2025.127750","url":null,"abstract":"<div><h3>Introduction</h3><div>Lung cancer is the number one cause of cancer death and survival rates have not significantly improved for decades. The underlying mechanism of lung cancer remains elusive, but it is known chromosome instability, altered DNA, and dysregulated cellular energetics are key to cancers. However, the importance of energetics during chemical carcinogenesis is unexplored.</div></div><div><h3>Methods</h3><div>We exposed human lung cells to hexavalent chromium [Cr(VI)], a known human respiratory carcinogen, for 24 or 120 h. Then, we performed untargeted metabolomics and analyzed changes with concentration and time through differential metabolomics.</div></div><div><h3>Results</h3><div>There was a distinct metabolic response with increasing Cr(VI) concentrations and a difference in the metabolic response with time, where metabolic enrichment of amino acid metabolism and nucleotide metabolism were affected with time and Cr(VI) treatment.</div></div><div><h3>Conclusions</h3><div>This study provides the first link between energy metabolism responses and chemical carcinogenesis, providing the foundation for a more in-depth investigation into how Cr(VI) alters the metabolic response of cells to induce lung cancer. We anticipate our study will be a starting point for more in-depth investigations into the role of dysregulated cellular energetics during carcinogenesis, which will further establish these changes as a critical early-stage event in carcinogenesis.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127750"},"PeriodicalIF":3.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metallothionein 2A alleviates ulcerative colitis by inhibiting ferroptosis in intestinal epithelial cells with Tfrc downregulation","authors":"Siqi Hua ,&nbsp;Shuangshuang Song ,&nbsp;Chunhao Yang ,&nbsp;Jingwen Xu ,&nbsp;Zhonghua Li ,&nbsp;Bo Zhu ,&nbsp;Zichun Hua","doi":"10.1016/j.jtemb.2025.127746","DOIUrl":"10.1016/j.jtemb.2025.127746","url":null,"abstract":"<div><h3>Background</h3><div>Ferroptosis has emerged as a critical pathogenic mechanism in ulcerative colitis (UC). Metallothioneins (MTs), known for their redox regulatory functions, may protect against ferroptosis in UC. However, the underlying mechanisms are still unclear.</div></div><div><h3>Methods</h3><div>We conducted comprehensive investigations combining a bioinformatics analysis of a human UC dataset (GSE243625) with experimental validations using DSS-induced murine colitis models and RSL3-treated HCT116 human colon epithelial cells. Our experimental approaches included immunohistochemical analysis of MT2 expression, assessment of ferroptosis markers, evaluation of mitochondrial function, measurement of mitochondrial redox status and Fe²⁺, and profiling of protein expression. The therapeutic potential of ZnO nanoparticles was evaluated <em>in vivo</em>.</div></div><div><h3>Results</h3><div>Transcriptomic analysis revealed significant downregulation of all nine MT family members in UC patients, with the most pronounced suppression occurring in active disease. This finding was corroborated in murine models, which showed reduced colonic MT2 expression following DSS treatment. Cellular studies demonstrated that MT2A overexpression provides robust protection against RSL3-induced ferroptosis, evidenced by a reduction in lipid peroxidation, preservation of mitochondrial integrity, and decreased accumulation of mitochondrial reactive oxygen species. Mechanistically, MT2A modulated cellular iron homeostasis by downregulating transferrin receptor (Tfrc). Importantly, the oral administration of ZnO nanoparticles effectively restored MT2 expression, downregulated Tfrc, attenuated ferroptosis, and significantly alleviated colitis symptoms in vivo.</div></div><div><h3>Conclusions</h3><div>Our findings establish MT2A as a crucial regulator of ferroptosis in UC pathogenesis and identify ZnO nanoparticle-mediated MT2 induction and Tfrc downregulation as a promising therapeutic strategy. This study provides novel insights into the MT2A-ferroptosis axis and its potential translational applications for UC treatment.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127746"},"PeriodicalIF":3.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unexpected arsenic compound in routine determination of arsenic species in urine","authors":"Peter Heitland ,&nbsp;Andrea Raab ,&nbsp;Siegfried Krell","doi":"10.1016/j.jtemb.2025.127745","DOIUrl":"10.1016/j.jtemb.2025.127745","url":null,"abstract":"<div><div>A validated method of anion exchange liquid chromatography coupled to inductively coupled plasma mass spectrometry (LC-ICP-MS) was applied to determine arsenic species in human urine, particularly for occupational and environmental health investigations. In addition to the chromatographic peaks of arsenobetaine (As-B), dimethylarsinate (DMA), methylarsonate (MA), As(III) and As(V), which occur regularly, we have observed an additional As peak overlapping with that of As(V) in several routine analyses. Further investigations identified this unknown As compound as p-arsanilic acid, which had contaminated the urine samples due to application of a test strip for rapid determination of certain clinical parameters before shipment of the samples to the laboratory. It is essential that urine samples for trace element analysis do not come into contact with test strips, an important prerequisite that is not always considered in subsequent occupational biomonitoring.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127745"},"PeriodicalIF":3.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of arsenic exposure through drinking water on pigment disorders: A meta-analysis and a Bayesian benchmark concentration analysis","authors":"Rujin Wang ,&nbsp;Xingrun Li ,&nbsp;Yuanjie Wei ,&nbsp;Tingxu Jin ,&nbsp;Yuanhui Zhu ,&nbsp;Ting Zhang ,&nbsp;Rui Yan ,&nbsp;Heran Li ,&nbsp;Koichi Kato ,&nbsp;Runa A ,&nbsp;Qianlei Yang ,&nbsp;Yan An","doi":"10.1016/j.jtemb.2025.127744","DOIUrl":"10.1016/j.jtemb.2025.127744","url":null,"abstract":"<div><h3>Background</h3><div>Skin lesions are an early and distinct symptom of waterborne arsenic poisoning, while pigment disorder is the first symptom of skin lesions. Therefore, an in-depth analysis of the association between pigment disorders and arsenic concentration in drinking water can be used as a basis for the development of arsenic drinking water standards. However, it remains unclear whether arsenic primarily causes depigmentation or hyperpigmentation, and what dose of arsenic is safe with respect to skin symptoms.</div></div><div><h3>Methods</h3><div>A meta-analysis was performed using Stata 17.0 software. PubMed, Embase, Web of Science, China National Knowledge Infrastructure (CNKI), VIP Chinese Journal Service Platform, and Wanfang Data databases were searched up to December 1, 2024. The Bayesian benchmark dose (BBMD) analysis system was used to estimate the benchmark dose (BMD) and its lower confidence limit (BMDL).</div></div><div><h3>Results</h3><div>Twelve articles with 36, 670 observations were included. The meta-analysis results indicated that arsenic exposure through drinking water was associated with pigment disorders, with hyperpigmentation being the most common manifestation. The BBMD results suggested that BMDs for the association between arsenic concentrations in drinking water and pigment disorders were determined to be 4.93 μg/L (BMDL1 = 4.85 μg/L), 6.54 μg/L (BMDL5 = 6.19 μg/L), 8.41 μg/L (BMDL10 = 7.80 μg/L) when using BMRs of 1 %, 5 %, and 10 %.</div></div><div><h3>Conclusions</h3><div>Hyperpigmentation is the most common manifestation caused by drinking water arsenic exposure and may link to keratoses and skin cancer. Arsenic in drinking water over 7.80 μg /L raises 10 % risk of pigment disorders, which can also lead to more severe keratosis and skin cancer. Based on these findings, three interim targets are proposed, providing a basis for the establishment of stricter arsenic limits in drinking water.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127744"},"PeriodicalIF":3.6,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative effect of zinc oxide nanoparticles and activated charcoal against the toxic effects of aflatoxins in ducks","authors":"Zakaria M. Zaky ,&nbsp;Tarek M. Sobhy ,&nbsp;Safwat Ali ,&nbsp;Nashwa Hamad ,&nbsp;Aly A. Othman ,&nbsp;Heba F. Kamaly","doi":"10.1016/j.jtemb.2025.127741","DOIUrl":"10.1016/j.jtemb.2025.127741","url":null,"abstract":"<div><div>To determine the potential toxic effects of aflatoxins (AFs), the ameliorative role of zinc oxide nanoparticles and activated charcoal against aflatoxicosis, and finally, the effective role of the recovery period against aflatoxins toxic effects and their residues. A total of 70 one-day-old Moulard ducklings were obtained and classified into seven groups, comprising 10 ducklings per group. The control group (G1) ducklings were fed on a basal diet free from AFs. Group 2 (G2) ducklings received a naturally AFs-contaminated feed with 50 ppb total AFs. Group 3 (G3) ducklings consumed 50 ppb AFs-contaminated feed and 10 mg/kg/feed zinc oxide nanoparticles (ZnONPs). Group 4 (G4) ducklings consumed 50 ppb AFs-contaminated feed and 1.5 % of the ration activated charcoal. Group 5 (G5) ducklings consumed 50 ppb AFs-contaminated feed with ZnONPs and an activated charcoal combination. Group 6 (G6) ducklings consumed a basal diet with ZnONPs. Group 7 (G7) ducklings consumed a basal diet with activated charcoal for 25 days, followed by an AF withdrawal period of 20 days as a recovery period through different groups of the experiment. Results showed an increase in feed intake, a decrease in growth rate, ruffled feathers, and brownish diarrhea in ducks that fed on AFs-contaminated feed during the first 25 days of the experiment. Alterations in hematological parameters and vital hepatic and renal functions in different groups of the experiment. There was a significant increase in the percentage of DNA damage to the liver in G2 compared to the control group. Aflatoxins residues were analyzed and detected in ducks' liver and muscles of different groups in the experiment except G1, G6 and G7. Obvious histopathological lesions in the liver and bursa of fabricious of ducks that were fed on an AFs-contaminated diet for 25 days were detected in comparison with the normal architecture of the control and the nearly normal architecture of different groups in the experiment. The results also deduced the ameliorative role of zinc oxide nanoparticles, activated charcoal, and the recovery period against aflatoxicosis in ducks, where some biochemical disturbances and pathological lesions started to disappear gradually during this period.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127741"},"PeriodicalIF":3.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should stingrays be on the menu? Significant human health risks associated to the consumption of the endangered Groovebelly Stingray (Dasyatis hypostigma) in southeastern Brazil","authors":"Amanda Pontes Lopes ,&nbsp;Júlia Vianna de Pinho ,&nbsp;Regina Fonseca ,&nbsp;Carlos Adam Conte-Junior ,&nbsp;Tatiana Dillenburg Saint’Pierre ,&nbsp;Rachel Ann Hauser-Davis","doi":"10.1016/j.jtemb.2025.127743","DOIUrl":"10.1016/j.jtemb.2025.127743","url":null,"abstract":"<div><h3>Introduction</h3><div>Elasmobranchs, a group encompassing sharks and rays, face significant threats worldwide due to capture as bycatch and environmental contamination. They are, however, highly consumed in several regions, including in Southeastern Brazil, potentially leading to human health risks. This is the case for the endangered Groovebelly Stingray (<em>Dasyatis hypostigma</em>), although no studies assessing human health risks due to the consumption of this species are available to date.</div></div><div><h3>Methodology</h3><div>In this sense, several metals and metalloids were analyzed in the muscle tissue of 28 Groovebelly Stingray specimens sampled incidentally from Rio de Janeiro, Brazil, and the main human health risk indices were calculated. A total of 16 elements were evaluated, including Hg and MeHg, plus a best and worst-case scenario of inorganic As levels of 1 % and 10 %, for all proposed risk indices, human age groups, and sexes.</div></div><div><h3>Results</h3><div>Differences in risk are evident between age groups, even at the lowest intake frequency of once a week. Furthermore, all calculated risks showed worrying results at some point, whether in relation to consumption frequency, element or sex. The most alarming scenario is noted for As, where the worst-case scenario (As 10 %) for the EDI for infants reached more than 8-fold the adult values for men and 7-fold the adult values for women.</div></div><div><h3>Conclusion</h3><div>These findings indicate that the meat of this endangered ray should not be consumed, also comprising an indirect conservation strategy, as awareness of these risks can encourage safer eating practices by consumers.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127743"},"PeriodicalIF":3.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vanadium shows no effect in stress-induced hyperthermia and the tail suspension test in healthy mice","authors":"Magdalena Sowa-Kućma ,&nbsp;Patrycja Pańczyszyn-Trzewik ,&nbsp;Natalia Gałka ,&nbsp;Bartosz Bobula ,&nbsp;Katarzyna Stachowicz","doi":"10.1016/j.jtemb.2025.127742","DOIUrl":"10.1016/j.jtemb.2025.127742","url":null,"abstract":"<div><div>Vanadium (V) is a trace element in the environment; it is detected in soil, water, air, dust, and food products. V-containing compounds have shown therapeutic potential in the treatment of diabetes. However, studies on the effects of V on animal behavior remain limited and sporadic. This study investigates the impact of acute (0.2, 2, 5 mg/kg, and 20 mg/kg) or repeated V administration (2 mg/kg/day; 7 days) in mice, employing stress-induced hyperthermia (SIH) as the primary behavioral assay. Additionally, its influence was assessed through single-dose administration in the tail suspension test (TST) and the Rota-rod test. The findings indicate no significant effects of V on the studied parameters, although motor coordination was notably impaired. Electrophysiological experiments were conducted to further elucidate vanadium's influence on neuronal function to assess its effects on long-term potentiation (LTP), a key process in synaptic plasticity, revealing no discernible impact. Moreover, the expression levels of gamma-aminobutyric acid (GABA) receptors, specifically GABAA1, GABAB1, and GABAB2, as well as glutamic acid decarboxylase (GAD67), were analyzed in the frontal cortex (FCx) using western blotting. Collectively, the results indicate a negligible influence of V on glutamatergic transmission and GABAergic receptor activity, after a single administration to healthy mice.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"91 ","pages":"Article 127742"},"PeriodicalIF":3.6,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}