Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Quercetin attenuates cadmium-induced hepatotoxicity by suppressing oxidative stress and apoptosis in rat","authors":"Yaning Shi ,&nbsp;Ke Wang ,&nbsp;Hao Ling ,&nbsp;Junbing Mao ,&nbsp;Bing Xu ,&nbsp;Zongping Liu ,&nbsp;Jicang Wang","doi":"10.1016/j.jtemb.2024.127554","DOIUrl":"10.1016/j.jtemb.2024.127554","url":null,"abstract":"<div><h3>Background</h3><div>Cadmium (Cd) is considered a major industrial and environmental toxicant, threatening the health of aquatic organisms, plants, animals, and humans. Quercetin (Que) is a natural flavonoid with antioxidant properties. The purpose of this study was to investigate the role of the oxidative stress and apoptosis in Cd-induced hepatotoxicity and the protective effect of Que.</div></div><div><h3>Methodology</h3><div>Thirty-six male SD rats were randomly divided into 6 groups: control group, 1 mg/kg Cd group, 2 mg/kg Cd group, 1 mg/kg Cd+Que group, 2 mg/kg Cd + Que group, and a Que group. After a feeding period of 28 days, serum and liver tissue samples were collected to evaluate liver function, oxidative stress levels, liver histology, and apoptosis.</div></div><div><h3>Results</h3><div>Experimental results confirmed that compared with the control group, the body weights of the Cd group significantly decreased. Additionally, there was a tremendous increased in the levels of ALT, AST, and LDH, and a significant decreased in the activities of SOD, CAT, and GSH content, while the level of MDA increased. Pathological sections of the liver showed that Cd-induced rats had ruptured liver tissue cells, exposed nuclei, and disturbed arrangement of hepatocyte cords. Cd exposure decreased the mRNA and protein expression of Nrf2 and NQO1 while increased the mRNA and protein expression of Keap1, thereby inducing oxidative stress. Meanwhile, Cd exposure increased the mRNA and protein expressions of Cytc, caspase-9, caspase-3, and Bax, while decreased the expression of Bcl-2. Conversely, after Que addition of alleviated liver injury and oxidative stress induced by Cd and inhibited apoptosis.</div></div><div><h3>Conclusion</h3><div>In conclusion, Que alleviates hepatic toxicity induced by Cd through suppression of oxidative stress and apoptosis.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127554"},"PeriodicalIF":3.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The elemental variance between the \"rice\" and \"non-rice\" portions of Maifanitum and its health risk assessment","authors":"Liu Zhou ,&nbsp;Zheng Liu ,&nbsp;Yulu Ma ,&nbsp;Fang Fang ,&nbsp;Xilong Qian ,&nbsp;Yanqiong Pan ,&nbsp;Ying Zhang ,&nbsp;Xiuxiu Wang ,&nbsp;Jing Zhao ,&nbsp;Shengjin Liu","doi":"10.1016/j.jtemb.2024.127550","DOIUrl":"10.1016/j.jtemb.2024.127550","url":null,"abstract":"<div><h3>Background</h3><div>Maifanitum, a mineral used in Chinese medicine, was first documented during the Song Dynasty (960–1279). Historical records suggest its multifaceted therapeutic properties, including detoxification and stasis resolution, necrosis removal and tissue regeneration, diuretic and calculi dissolution and prolonging life. The concentration of elements in Maifanitum may vary depending on its origin, different parts, which can affect its effectiveness in different fields of applications. Therefore, the analysis of elements in Maifanitum and the subsequent health risk assessment have been conducted. This provides an important basis for the quality control and application safety of Maifanitum.</div></div><div><h3>Method</h3><div>The analytical techniques employed in this study are inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES), utilized for the quantitative assessment of 60 elements (Refer to <span><span>Appendix 1</span></span>) within Maifanitum samples. Based on the test results, chemometric methods are employed to evaluate the characteristics and differences in elemental concentration from different sources and locations. Additionally, a preliminary health risk assessment is conducted for Maifanitum from different origins and various parts.</div></div><div><h3>Results</h3><div>We have established a fingerprint of the elements within Maifanitum, demonstrating a commendable level of similarity. The findings from hierarchical cluster analysis（HCA） corroborated with those from principal component analysis (PCA), collectively unveiling a systematic profile of elemental disparities between Maifanitum samples of diverse origins and applications. It also revealed that there are differences in the concentration of Al, Ga, Be, Hf, Na, Sn, Ti, Zr, Gd, Tb, Sr, Pb, Ce, Ba and other elements in different parts of Maifanitum. While Cd, As, and Cu levels in all samples were within the permissible limits as defined by the Chinese Pharmacopeia, Pb concentrations in the majority of samples were found to surpass these standards, albeit slightly in the ''non-rice'' fraction. The assessment of both beneficial and deleterious elements indicates that the ''non-rice'' fraction of Maifanitum possesses superior quality attributes. Moreover, the overall concentration of rare earth elements in Maifanitum is substantially below the established lower threshold for daily human consumption, with no immediate evidence suggesting any adverse health risks.</div></div><div><h3>Conclusion</h3><div>This study provides a basis for the quality control and safety evaluation of Maifanitum in clinical use.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127550"},"PeriodicalIF":3.6,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142515473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper dyshomeostasis and its relationship to AMPK activation, mitochondrial dynamics, and biogenesis of mitochondria: A systematic review of in vivo studies","authors":"Sarah Maria van Tol Amaral Guerra ,&nbsp;Letícia Cordeiro Koppe de França ,&nbsp;Katriane Neto da Silva,&nbsp;Fabielly Scolari Grotto,&nbsp;Viviane Glaser","doi":"10.1016/j.jtemb.2024.127549","DOIUrl":"10.1016/j.jtemb.2024.127549","url":null,"abstract":"<div><h3>Introduction</h3><div>Copper dyshomeostasis can be related to an increase in copper levels, resulting in toxicity, or to a decrease in tissues levels, impairing cuproenzyme activities. Inside cells, copper can be found in the cytoplasm and inside organelles, and the main organelle that compartmentalizes copper is the mitochondrion. This organelle can form networks and may fuse or fission from this, determining the mitochondrial fusion and fission processes, respectively. Together with mitophagy (autophagy of mitochondria) and mitochondrial biogenesis, mitochondrial fusion and fission (denominated mitochondrial dynamics) determine the number of mitochondria in a cell. A master regulator of mitochondrial dynamics and biogenesis of new mitochondria is AMPK. Considering that both a decrease and an increase in copper levels can influence mitochondrial turnover, especially in diseases related to copper dyshomeostasis, the objective of this systematic review was to verify the current knowledge on the influence of copper homeostasis on AMPK activation, mitochondrial dynamics, and biogenesis of new mitochondria <em>in vivo</em>.</div></div><div><h3>Methods</h3><div>PubMed (MEDLINE), Embase, and Web of Science databases were used to search for articles in the literature. Data about the effects of a decrease or an increase in copper levels on the expression of proteins involved in mitochondrial dynamics or biogenesis, and data about AMPK and p-AMPK levels were extracted.</div></div><div><h3>Results</h3><div>Meta-analysis has demonstrated that high copper levels increase mitochondrial fission and inhibit mitochondrial fusion. Additionally, an increase in copper levels results in AMPK activation. Few studies have analyzed the effects of high copper levels on proteins related to mitochondrial biogenesis, as well as the impact of a decrease in this metal on mitochondrial dynamics and biogenesis, and on AMPK activation.</div></div><div><h3>Conclusions</h3><div>Despite the results gathered in this review, other studies are necessary to completely understand the role of copper in regulating AMPK activation, mitochondrial dynamics, and the biogenesis of new mitochondria, since the cell response to a copper dyshomeostasis could be different depending on the species and tissues analyzed.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127549"},"PeriodicalIF":3.6,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Water-soluble organic selenometabolites of alfalfa (Medicago sativa L.) green biomass-derived fractions","authors":"Éva Domokos‑Szabolcsy ,&nbsp;Áron Soós ,&nbsp;Béla Kovács ,&nbsp;Zoltán Kovács ,&nbsp;Mihály Dernovics","doi":"10.1016/j.jtemb.2024.127545","DOIUrl":"10.1016/j.jtemb.2024.127545","url":null,"abstract":"<div><h3>Background</h3><div>Tolerance of plants towards selenium, a non-essential microelement for higher plants, is a key issue when designing either the indirect (selenium-depletion from highly seleniferous soils) or directed (selenized feed production) enrichment of selenium in forages. Alfalfa (<em>Medicago sativa</em> L.), the well-known forage crop of the Fabaceae family, has been gaining considerable interest due to its application as a green manure, as a cover crop, or in soil remediation by nitrogen fixation.</div></div><div><h3>Objective</h3><div>The goal of our study was to assess into which selenocompounds alfalfa plants biotransform the excess selenium uptake from the soil. Selenocompounds (other than selenomethionine and inorganic forms) accumulated in the fiber and the so-called brown juice by-product fractions of processed alfalfa biomass were targeted.</div></div><div><h3>Methodology</h3><div>Inductively coupled plasma – mass spectrometry assisted multidimensional (strong anion exchange, strong cation exchange, reversed phase) orthogonal chromatographic purification was applied to supply Se-containing fractions in adequately high purity for electrospray high-resolution mass spectrometry (used for the first time for this matrix) analyses.</div></div><div><h3>Results</h3><div>As a total, 30 selenocompounds (with isomers) were described, showing the abundance of the derivatives of selenohexose, selenohomolanthionine, and 2,3-dihydroxypropionic acid. Out of the 30 selenocompounds, 15 could be assigned the elemental composition, and the tentative structure of five compounds including among others deamino-2-oxo-selenohomolanthionine, deamino-hydroxy-selenohomolanthionine, and the dimer of 2,3-dihydroxypropionyl-selenohomocysteine could be presented.</div></div><div><h3>Conclusions</h3><div>The studied fractions arising from the standard alfalfa processing technology contained a wide variety of selenocompounds whose origin can be either the plant’s inherent Se metabolism or the processing technology itself. The importance of negative mode data acquisition has been highlighted, as out of the 30 compounds, 16 could be detected exclusively in this electrospray ionization mode.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127545"},"PeriodicalIF":3.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between magnesium depletion score and depression among individuals aged 20 to 60 years","authors":"Zongao Cai ,&nbsp;Jiachen She ,&nbsp;Xiaozhu Liu ,&nbsp;Ruihui Li ,&nbsp;Shuang Guo ,&nbsp;Zeyang Han ,&nbsp;Jiyang Zhou ,&nbsp;Hairong Zhang ,&nbsp;Yudi Xu ,&nbsp;Ge Zhang ,&nbsp;Zhaokai Zhou ,&nbsp;Xueli Guo ,&nbsp;Shiyong Wu","doi":"10.1016/j.jtemb.2024.127543","DOIUrl":"10.1016/j.jtemb.2024.127543","url":null,"abstract":"<div><h3>Background</h3><div>Magnesium is closely associated with depression. The study aims to explore the relationship between magnesium depletion score (MDS), used to evaluate the body’s magnesium deficiency status, and depression.</div></div><div><h3>Methods</h3><div>This population-based cross-sectional study used data from the National Health and Nutrition Examination Survey in 2005–2018 and included adults aged 20 to 60 years. Depression was determined using the Patient Health Questionnaire-9 (PHQ-9). MDS was a practical assessment instrument used to assess the status of magnesium deficiency. Multivariable logistic and restricted cubic spline models were used to assess the associations between MDS and depression.</div></div><div><h3>Results</h3><div>Among the 18247 adults, 1753 participants were diagnosed with depression. Multivariable logistic regression analysis indicated that when MDS was treated as a continuous variable, each one-unit increase in MDS was associated with an odds ratio (OR) for depression of 1.15 (1.04, 1.26). When MDS was considered a categorical variable, compared to participants with MDS of 0, the ORs for depression for those with MDS of 1, 2, and ≥3 were 1.03 (0.87, 1.21), 1.41 (1.12, 1.78), and 1.58 (1.06, 2.35), respectively. Restricted cubic spline analysis showed that there was no non-linear relationship between MDS and the prevalence of depression. Subgroup analyses indicated the positive associations between MDS and depression were generally similar in different populations.</div></div><div><h3>Conclusions</h3><div>MDS may be positively associated with the prevalence of depression. In the future, additional longitudinal studies are needed to validate our findings and investigate potential mechanisms.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127543"},"PeriodicalIF":3.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of molybdenum in ameliorating busulfan-induced infertility in female mice","authors":"Feng-jun Liu ,&nbsp;Yu-ling Zhang ,&nbsp;Xiao-shan Wang ,&nbsp;Ya-qin Zhao ,&nbsp;Hong-wei Wang","doi":"10.1016/j.jtemb.2024.127546","DOIUrl":"10.1016/j.jtemb.2024.127546","url":null,"abstract":"<div><h3>Background</h3><div>Molybdenum (Mo) plays a crucial role in regulating normal physiological function. However, its potential effect on female infertility has received little attention.</div></div><div><h3>Methods</h3><div>In this study, we explored the potential molecular mechanisms of Mo’s action on mouse ovaries and oocytes by establishing a busulfan-induced infertility model. Adult female Kunming mice were randomly divided into three groups: control, +busulfan, and +busulfan+Mo. After 30 days of busulfan treatment [Myleran, 20 mg/kg body weight ip], mice in the busulfan+Mo group were provided with 7.5 mg/L Mo per day in drinking water for an additional 42 days. On day 72, we examined the morphology of the oocytes and ovarian tissue after H&amp;E staining, measured the concentrations of serum hormones by ELISA, and detected Bax, Bcl-2, caspase-3 and caspase-9 by immunohistochemical staining and western immunoblotting. We also assessed the oxidative stress in cells by measuring the activity of the antioxidant enzyme, SOD, the concentrations of MDA and LDH, and the percentage of apoptotic cells using kits. The number of litters born was counted after mating with male mice, and the organ coefficients were calculated after weighing on an analytic balance.</div></div><div><h3>Results</h3><div>Results showed that Mo treatment restored female reproductive hormone levels to near normal. Mo also significantly inhibited the mitochondrial stress-induced expression of apoptotic proteins.</div></div><div><h3>Conclusion</h3><div>Our findings demonstrate that Mo treatment at a dose of 7.5 mg/L can ameliorate busulfan-induced infertility in female mice. These data may provide a reference for the development of treatments for female infertility.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127546"},"PeriodicalIF":3.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142484448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Health risk assessment of lead and cadmium exposure from food and snuff in Pakistani population","authors":"Hamza Shafiq ,&nbsp;Mamoona Amir ,&nbsp;Salman Asghar ,&nbsp;Aneela Hameed ,&nbsp;Muhammad Riaz","doi":"10.1016/j.jtemb.2024.127544","DOIUrl":"10.1016/j.jtemb.2024.127544","url":null,"abstract":"<div><h3>Background</h3><div>Smokeless tobacco (SLT) is a tobacco-based product consumed without burning or smoking. Snuff, commonly known as <em>naswar,</em> is a widely used SLT product in Pakistan. This study is designed to investigate the concentrations of lead (Pb) and cadmium (Cd) in snuff and their associated health risks to consumers.</div></div><div><h3>Methods</h3><div>Blood samples from snuff consumers and non-consumers were analyzed for Pb and Cd concentrations. Additionally, samples of drinking water and wheat flour were also analyzed for Pb and Cd. Health risk assessments were calculated using hazard quotient (HQ) and incremental lifetime cancer risk (ILCR) for Pb and Cd.</div></div><div><h3>Results</h3><div>Snuff consumers had significantly higher blood-Pb and blood-Cd levels compared to non-consumers. Approximately 38.5 % of snuff consumers were found to exceed blood-Pb levels from the safe limits (10 µg dL⁻¹) of the World Health Organization (WHO), while 48.9 % of snuff consumers were found to exceed blood-Cd levels from the WHO safe range of 0.03–0.12 µg dL⁻¹. Snuff samples contained Pb and Cd concentrations ranging from 5.3 to 22.4 µg g⁻¹ and 0.5–2.2 µg g⁻¹, respectively. Drinking water and wheat flour samples were found within the safe limits for Pb and Cd concentration, suggesting snuff as the primary source of exposure. Health risk assessments indicated elevated HQ values, for Pb (38.9 %) and Cd (14.3 %) detected in snuff, surpassing the safe HQ threshold of 1. Concerning ILCR from snuff, values for Pb were within the acceptable range, but alarmingly 100 % of samples for Cd exceeded the safe limits of USEPA (1.0×10⁻⁴), indicating a significant cancer risk in snuff users.</div></div><div><h3>Conclusion</h3><div>This study highlights the exceeded Pb and Cd concentrations in snuff as well as also reporting significant carcinogenic and non-carcinogenic health risks to consumers. These findings emphasized the urgent need for regulatory bodies to ensure the safety of snuff before selling in the market. Public Health Professionals should also initiate educational campaigns to raise awareness among consumers and manufacturers about various health risks associated with the snuff.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127544"},"PeriodicalIF":3.6,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The overlooked impact of cadmium on the progression of chronic hepatitis and the onset of renal failure in advanced cirrhosis","authors":"Ana Cirovic ,&nbsp;Soisungwan Satarug ,&nbsp;Jovan Jevtic ,&nbsp;Ana Ivanovski ,&nbsp;Orish E. Orisakwe ,&nbsp;Sasa Jankovic ,&nbsp;Aleksandar Cirovic","doi":"10.1016/j.jtemb.2024.127542","DOIUrl":"10.1016/j.jtemb.2024.127542","url":null,"abstract":"<div><div>The mechanism of hepatocyte destruction in chronic hepatitis is not completely understood, while renal failure in individuals with advanced cirrhosis is a significant concern. It is well known that smokers who are chronically infected with hepatitis B and C viruses (HBV, HCV) have a poor prognosis. In the present review, we propose a novel hypothesis that environmental exposure to a nephrotoxic metal pollutant, cadmium (Cd) may contribute to hepatocyte destruction and, subsequently, affect the duration of chronic hepatitis. The metal binding protein, metallothionein (MT) sequesters cadmium as CdMT complexes, and effectively neutralize its adverse effects. Cadmium can cause the damage to hepatocytes, only when it is in an unbound form. In addition to its ability to bind cadmium, MT can act as a scavenger of reactive oxygen species (ROS). However, the cellular MT levels may decrease, when ROS is excessively produced under the pathologic chronic viral hepatitis conditions, especially while the cellular levels of zinc may also be low. Zinc is an endogenous inducer of MT, and is required for maximal MT expression. High ROS levels in the hepatocytes diminishes MT binding to metals. Consequently, the proportion of unbound Cd is increased and thus there is more hepatic damage. Hepatic damage leads to a copious release of CdMT into the circulation. This significant cadmium load, which occurs after hepatic damage, and in some cases, muscle atrophy, induces kidney damage with resultant renal failure in advanced cirrhosis.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127542"},"PeriodicalIF":3.6,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142421895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary zinc status is associated with ZnT3 (SLC30A3), IL-6 gene expressions and spinal cord tissue damage in spinal cord tissue in a cuprizone-induced rat Multiple Sclerosis model","authors":"Aysenur Feyza Bayiroglu ,&nbsp;Gozde Acar ,&nbsp;Elif Gulbahce-Mutlu ,&nbsp;Saltuk Bugra Baltaci ,&nbsp;Rasim Mogulkoc ,&nbsp;Abdulkerim Kasim Baltaci","doi":"10.1016/j.jtemb.2024.127540","DOIUrl":"10.1016/j.jtemb.2024.127540","url":null,"abstract":"<div><div>The aim of this study was to investigate the effects of dietary zinc status on spinal cord tissue damage and ZnT3, IL-6 gene expressions in a cuprizone-induced rat Multiple Sclerosis (MS) model. The study was carried out on 46 adult male rats of the genus Wistar. The animals used in the study were divided into 5 groups (G) (Control 6, other groups 10). G1, Control. G2, Sham-MS: Carboxy-methyl-cellulose (KMS) solution in which Cuprizon was dissolved was given to rats by gavage daily for 8 weeks at the rate of 1 % of daily feed consumption. MS was formed by giving 1 % of the daily feed consumption cuprizon in KMS solution by gavage to the animals in G3, 4 and 5 for 8 weeks. G4 was fed with a zinc deficient (50 µg/kg zinc) diet. G5 was given intraperitoneal (ip) zinc sulfate (5 mg/kg/day) supplementation. MS formation in animals was determined by Rotarod tests and Myelin Basic Protein (MBP) gene expression analysis. ZNT3 and IL-6 gene expression levels in spinal cord tissue samples of animals by Real-Time-PCR method; MDA and GSH levels were determined by ELISA method. The highest spinal cord MDA and IL-6 levels were obtained in G3 and G4 (P&lt;0.05). Zinc supplementation in G5 prevented the increase in the mentioned parameters and turned them into control values (P&lt;0.05). The spinal cord GSH and ZnT3 levels of G3 and G4 were lower than all other groups (P&lt;0.05). Zinc supplementation prevented suppression in the same parameters in G5 and reached the control values (P&lt;0.05). The findings of the current study suggest that zinc supplementation in addition to treatment for MS may be beneficial in reducing the severity of the disease.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127540"},"PeriodicalIF":3.6,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenoprotein S (SELENOS) is a potential prognostic biomarker for brain lower grade glioma","authors":"Yuetong Wang ,&nbsp;Kai Qu ,&nbsp;Zengrun Xia ,&nbsp;Meng Qi ,&nbsp;Xiaoping Du ,&nbsp;Zunhua Ke ,&nbsp;Rongqiang Zhang","doi":"10.1016/j.jtemb.2024.127539","DOIUrl":"10.1016/j.jtemb.2024.127539","url":null,"abstract":"<div><h3>Background</h3><div>Selenium, an essential micronutrient, primarily exists as selenocysteine in various selenoproteins. Selenoprotein S (SELENOS) is crucial in the development of human cancer. This study aimed to explore the correlation between SELENOS gene expression and the prognosis of brain lower-grade glioma (LGG).</div></div><div><h3>Methods</h3><div>SELENOS protein and mRNA expression in human normal and tumor tissues were explored through the HPA database. SELENOS expression differences between normal and tumor tissues, along with its prognostic significance in gliomas, were analyzed using the TCGA, GTEx datasets, while the CGGA dataset was used to further assess its prognostic potential in a Chinese cohort. The association between SELENOS expression and tumor immune infiltration was also assessed. Multivariate and univariate Cox models were used to screen for clinicopathological parameters associated with SELENOS expression. The GDSC datasets was utilized to explore the connection between SELENOS and chemotherapeutic responses in LGG. A protein-protein interaction network for SELENOS was created. SELENOS expression in LGG cell lines were determined by Western blotting and qRT-PCR, and its functions were ascertained by routine <em>in vitro</em> experiments.</div></div><div><h3>Results</h3><div>SELENOS was upregulated in 11 cancers and downregulated in 10 cancers relative to the corresponding normal tissues, and correlated significantly with the prognosis, especially for GBM, LGG and GBMLGG. Furthermore, It displayed a positive correlation with immune cell infiltration levels in LGG. Multivariate and Univariate Cox analyses confirmed that the impact of SELENOS on the prognosis of LGG is the combined result of factors such as age and tumor grade. The expression of SELENOS was significantly negatively correlated with temozolomide IC50 in LGG. We found that SELENOS interacts with 10 proteins, which are upregulated in LGG compared to human normal tissues. The expression of these interactors is positively correlated with SELENOS expression and LGG survival/prognosis. <em>In vitro</em> experiments confirmed the aberrant expression of SELENOS in LGG cell lines, and siRNA-mediated knockdown of SELENOS reduced the proliferation, viability, invasion and migration of LGG cells, and induced apoptosis.</div></div><div><h3>Conclusions</h3><div>SELENOS is a potential prognostic marker and therapeutic target for LGG, and its low expression is associated with favorable prognosis in LGG.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"86 ","pages":"Article 127539"},"PeriodicalIF":3.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}