Journal of Trace Elements in Medicine and Biology最新文献

{"title":"Evaluation of M86 and M87 compounds against lead, cadmium, and arsenic toxicity in 2D and 3D liver cell models","authors":"Hamdi Temel ,&nbsp;Serap Yalçın Azarkan ,&nbsp;Serkan Şahin","doi":"10.1016/j.jtemb.2025.127665","DOIUrl":"10.1016/j.jtemb.2025.127665","url":null,"abstract":"<div><div>Increasing environmental pollution increases the risk of human exposure to toxic metals. Therefore, there is a need for substances to protect individuals against the harmful effects caused by toxic metals. Bu çalışma, 3-metoksi katekol bileşiğinin 1,4-fenil diboronik asitle 1:1 ve 1:2 mol ratios of 3-methoxy catechol compound with 1,4-phenyl diboronic acid (1:1 and 1:1) and 1,4-bis(4-methoxybenzo[<em>d</em>][1,3,2]dioxaborol-2-yl)phenyl)boronic acid (M86) and 1,4-bis(4-methoxybenzo[<em>d</em>][1,3,2]dioxaborol-2-yl)benzene (M87) against lead (Pb), cadmium (Cd) and arsenic (As) toxicity in THLE-2 liver cell line. The structures of synthesized compounds M86 and M87 were characterized by 1 H, 13 C NMR, LC-MS-IT-TOF, UV-Vis., FTIR. The biological activities of these compounds were evaluated by DPPH, ABTS, CUPRAC, anticholinesterase, antiurease and antithyrosinase tests. 2D and 3D cell models were used in THLE-2 cell line. The protective effects of M86 and M87 against Pb, Cd and As toxicity were examined by XTT test and ATP colorimetric method and IC50 values were determined. In antioxidant tests, it was observed that M86 and M87 exhibited high activity in ABTS, DPPH and CUPRAC tests compared to standard antioxidants ?-tocopherol (?-TOC) and butylated hydroxytoluene (BHT). Enzyme inhibition tests showed that M86 and M87 significantly suppressed acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzyme activities. These compounds were found to reverse the decrease in cell proliferation following Pb, Cd and As exposure. In conclusion, M86 and M87 have the potential to be versatile therapeutic agents that provide effective protection against metal toxicity. In the future, with the evaluation of the efficacy of these compounds in in vivo models and clinical studies, it is thought that their use against metal toxicity may be possible.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127665"},"PeriodicalIF":3.6,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of multi-metal exposure with hyperuricemia in middle-aged and older adults: A cross-sectional study in rural Guangxi, China","authors":"Qinyi Guan ,&nbsp;Kaisheng Teng ,&nbsp;Qiumei Liu ,&nbsp;Songju Wu ,&nbsp;Lei Luo ,&nbsp;Linhai Zhao ,&nbsp;Jiahui Rong ,&nbsp;Wenjia Jin ,&nbsp;Tiantian Zhang ,&nbsp;Mo Xiaoting ,&nbsp;Cai Jiansheng ,&nbsp;Zhiyong Zhang ,&nbsp;Jian Qin","doi":"10.1016/j.jtemb.2025.127670","DOIUrl":"10.1016/j.jtemb.2025.127670","url":null,"abstract":"<div><h3>Background</h3><div>Hyperuricemia constitutes a significant global public health concern, particularly among the elderly, and is associated with environmental factors such as metal exposure. In China, the predominant focus of existing research has been on urban populations, which leaves the relationship between hyperuricemia and ecological exposures in rural populations inadequately explored. This study investigates the association between hyperuricemia and exposure to multiple metals among elderly residents in rural China.</div></div><div><h3>Methods</h3><div>Cross-sectional survey data were collected from middle-aged and elderly individuals residing in Yao Autonomous County of Gongcheng, Guangxi, China, from 2018 to 2019. The analytical techniques employed included least absolute shrinkage and selection operator (LASSO) regression, binary logistic regression and Bayesian kernel machine regression (BKMR) to examine the association between exposure to multi-metal mixtures and hyperuricemia.</div></div><div><h3>Results</h3><div>Logistic regression results indicated a negative association between magnesium (OR=0.634, 95 % CI=0.476‐0.867, Q3) and hyperuricemia, while iron, nickel, strontium, molybdenum, and tin were positively associated with hyperuricemia (all P &lt; 0.05). The Bayesian kernel machine regression analysis revealed a significant overall positive correlation between hyperuricemia and plasma polymetallic mixtures, with notable contributions from Sr (PIP=0.991) and Fe (PIP=0.749), particularly when all metals were at their respective 50th percentiles. Exposure-response relationships were consistent with the logistic regression results. Plasma Sr was positively correlated with hyperuricemia at different levels of other metals (p25, p50, p75).</div></div><div><h3>Conclusion</h3><div>This study suggests that exposure to multi-metal mixtures is positively associated with hyperuricemia, with Sr and Fe being the most significant contributors.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127670"},"PeriodicalIF":3.6,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First record of trace elements in cetaceans stranded along the Asturias Coastline, Northwest Spain: One great diversity area of marine mammals","authors":"L. Baños-Doménech ,&nbsp;N. García-García ,&nbsp;J. Peñalver ,&nbsp;J.A. Armenteros ,&nbsp;E. Martínez-López","doi":"10.1016/j.jtemb.2025.127669","DOIUrl":"10.1016/j.jtemb.2025.127669","url":null,"abstract":"<div><div>The concentrations of cadmium (Cd), lead (Pb), arsenic (As), selenium (Se) and mercury (Hg) in the kidney, liver, brain and muscle of 20 individuals of cetaceans, stranded along the Asturias coastline between 2018 and 2020, have been studied. Species studied include fin whale (<em>Balaenoptera physalus</em>), striped dolphin (<em>Stenella coeruleoalba</em>), common dolphin (<em>Delphinus delphis</em>), bottlenose dolphin (<em>Tursiops truncatus</em>), harbour porpoise (<em>Phocoena phocoena</em>) and pygmy sperm whale. (<em>Kogia breviceps</em>). All trace elements analyzed were detected in all cetacean families. The most detected compound was Se in the kidney (90 %), closely followed by Hg in muscle (85 %), while the least detected metal was Pb in the kidney (5 %). The highest concentrations of Se (66.00 mg/kg), Hg (44.00 mg/kg) and As (2.00 mg/kg) were detected in the liver, followed by Cd (53.00 mg/kg) in the kidney and Pb (0.37 mg/kg) in the brain. The family that presented the highest concentrations of toxic metals was <em>Delphinidae</em>, compared to the family <em>Balaenopteridae,</em> which is explained by the feeding and behavioural habits of odontocetes. The concentrations found are lower than those detected in other areas of Spain and the Atlantic Ocean and lower than those considered responsible for toxic effects in cetaceans. In addition, low levels of metal contamination (IMP) were found in most samples, although in two individuals we cannot exclude effects at the renal level due to metal accumulation. The overall effect of biologically relevant variables on metal concentrations was assessed by generalised linear modelling (GLM) analysis, with tissue being the most influential variable on metal concentrations. In addition, the Hg:Se molar ratio was lower than 1 in most samples analyzed, so the detoxifying action of Se against Hg is confirmed. As it represents the first record of trace element analysis in cetaceans of this area, our study supplies useful data for the development of conservation strategies in the Cantabrian Sea, as well as provides valuable information for future research.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127669"},"PeriodicalIF":3.6,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacotherapeutic role of astringin against chromium induced nephrotoxicity via modulating TLR4/MyD88, HMGB1/RAGE and NF-κB pathway: A biochemical and pharmacokinetic approach","authors":"Han Hu ,&nbsp;Muhammad Faisal Hayat ,&nbsp;Syeda Sania Zahara ,&nbsp;Ali Akbar ,&nbsp;Khalid J. Alzahrani ,&nbsp;Khalaf F. Alsharif ,&nbsp;Fuad M. Alzahrani ,&nbsp;Ahmed Al-Emam","doi":"10.1016/j.jtemb.2025.127666","DOIUrl":"10.1016/j.jtemb.2025.127666","url":null,"abstract":"<div><h3>Background</h3><div>Chromium (Cr) is a noxious heavy metal that is reported to induce various organ damages including the kidneys. Astringin (ATN) is a polyphenolic flavonoid that demonstrates immense pharmacological potential.</div></div><div><h3>Aim</h3><div>This research was planned to assess the potential palliative efficacy of ATN against Cr induced renal toxicity via regulating biochemical and histological parameters.</div></div><div><h3>Methodology</h3><div>Thirty-two male albino (Sprague Dawley) rats were divided into four groups: the 1st group (control), 2nd group (Cr 15 mg/kg), 3rd group (Cr 15 mg/kg + ATN 10 mg/kg), and 4th (ATN 10 mg/kg) group. Gene profile was evaluated by using qRT-PCR protocol. The levels of other biochemical parameters were assessed through standard ELISA protocol as well as already reported standard assays. Histology was performed as per the basic principle of histopathology technique. The palliative role of ATN was further confirmed by molecular docking (MD) and molecular dynamic simulation (MDS) approach.</div></div><div><h3>Results</h3><div>Cr intoxication upregulated the gene expression of <em>high mobility group box1 (HMGB1), tumor necrosis factor- α (TNF-α), nuclear factor- kappa B (NF-κB), cyclooxygenase-2 (COX-2)</em>, <em>interleukin-6 (IL-6), myeloid differentiation primary response 88 (MYD88), receptor for advanced glycation end products (RAGE), toll-like receptor 4 (TLR4),</em> and <em>interleukin-1β (IL-1β)</em>. The levels of reactive oxygen species (ROS) and malondialdehyde were elevated while the activities of glutathione reductase (GSR), glutathione peroxidase (GPx), heme oxygenase-1 (HO-1), superoxide dismutase (SOD) &amp; catalase (CAT) as well as contents of glutathione (GSH) were reduced after Cr intoxication. Moreover, Cr exposure increased the levels of cystatin C, uric acid, neutrophil gelatinase-associated lipocalin (NGAL), creatinine, blood urea nitrogen (BUN), N-acetylglucosamine (NAG), kidney injury molecule-1 (KIM-1) &amp; urea while downregulating the concentrations of creatine clearance. Besides, the levels of B cell lymphoma-2 (Bcl-2) were reduced while the levels of cysteine-aspartic acid protease-9 (Caspase-9), Bcl-2–associated X protein (Bax) and cysteine-aspartic acid protease-3 (Caspase-3) were escalated after Cr intoxication. Renal tissues showed abnormal histology following the exposure to Cr. Nonetheless, ATN treatment effectively restored biochemical as well as histological impairments in renal tissues, thereby demonstrating the nephroprotective potential against Cr intoxication.</div></div><div><h3>Conclusion</h3><div>ATN therapy showed significant renal protection via suppressing oxidative stress, inflammation, apoptosis and histological damages. These findings emphasize the important role of ATN in regulating renal health via modulating TLR4/MyD88, HMGB1/RAGE and NF-κB signaling pathway.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127666"},"PeriodicalIF":3.6,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into ionic medicine: Cerium reduces the presence of reactive oxygen species and favors osteogenic over adipogenic differentiation in human mesenchymal stromal cells","authors":"F. Westhauser ,&nbsp;V. Jacobsen ,&nbsp;K. Zheng ,&nbsp;C. Merle ,&nbsp;A.R. Boccaccini ,&nbsp;T. Renkawitz ,&nbsp;E. Kunisch","doi":"10.1016/j.jtemb.2025.127668","DOIUrl":"10.1016/j.jtemb.2025.127668","url":null,"abstract":"<div><div>The guided application of metallic ions in bone tissue engineering (BTE) has recently gained popularity being described as one important example of ionic medicine (IM). BTE aims to enhance osteogenic differentiation of precursor cells like bone-marrow-derived mesenchymal stromal cells (BMSCs) and, by that, regenerate bone tissue. BMSCs however can also differentiate into adipogenic lineage. It is known that elevated levels of reactive oxygen species (ROS) stimulate BMSC towards (undesired) adipogenic differentiation. One ion, that is particularly interesting for application in IM-guided BTE is cerium (Ce) since it acts as a self-regenerating ROS-scavenger and has already been successfully incorporated in biomaterials. Ce has demonstrated pro-osteogenic, anti-adipogenic and anti-oxidative effects before, however, so far, there is no direct comparative study available that analyzes these effects on human BMSCs in one and the same setting. Therefore, in this study, the influence of Ce nitrate (CeN) on the expression of osteogenic, adipogenic and ROS-scavenging genes in BMSCs was evaluated as well as its impact on formation of an osseous extracellular matrix (ECM), lipid formation and physical ROS presence. The presence of CeN improved BMSCs viability, enhanced proliferation, and reduced ROS-levels. Furthermore, CeN suppressed adipogenesis while osteogenic differentiation and the formation and maturation of the ECM were enhanced. The presence of CeN reduced the physical presence of ROS and the gene expression patterns shifted towards an anti-oxidant profile. Ce therefore constitutes an attractive ion for application in IM-guided BTE. Further research is necessary to clarify the biological mechanisms and pathways that are involved in the Ce-mediated modulation of BMSC differentiation.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127668"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143917516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive assessment of matrix effects in metallomics: Evaluating 27 metals in serum, heparine-plasma-, EDTA-plasma and citrate-plasma by ICP-MS analysis","authors":"Giulia Guerra ,&nbsp;Elisabetta Venturelli ,&nbsp;Alessio Polymeropoulos ,&nbsp;Francesco Segrado ,&nbsp;Daniele Morelli ,&nbsp;Sabina Rinaldi ,&nbsp;Claudia Agnoli ,&nbsp;Sabina Sieri","doi":"10.1016/j.jtemb.2025.127667","DOIUrl":"10.1016/j.jtemb.2025.127667","url":null,"abstract":"<div><h3>Background</h3><div>Metallomics analysis is essential for studying environmental metal exposure and distinguishing between healthy and diseased individuals in large-scale studies. Inductively coupled plasma mass spectrometry (ICP-MS) is widely used due to its high sensitivity and ability to analyze multiple elements across a broad concentration range.</div></div><div><h3>Aim</h3><div>Developed to address key challenges in large-scale epidemiological studies, this method specifically focuses on evaluating the performance of metals measurement in biobank blood matrices that may not be optimized for metallomics, providing key insights for biobank planning</div></div><div><h3>Methods</h3><div>An ICP-MS-based analytical method using a \"dilute and shoot\" approach was developed to target 27 elements. The study assessed the behavior of these elements in serum and different plasma types (EDTA, citrate, and heparin) based on limits of detection (LOD), limits of quantification (LOQ), precision, accuracy, and quality controls.</div></div><div><h3>Results</h3><div>Heparin plasma and serum provided the most consistent measurements, with most elements exhibiting a coefficient of variation below 15 %. Citrated and EDTA plasma displayed higher variability, likely due to contamination from collection tubes and metal-anticoagulant interactions. Among 13 certified elements, Mg, K, Fe, Cu, Se, Co, and Ni matched reference values, while Ca, Zn, Cr, Mn, and Al were lower, and Hg was higher. Of the 14 elements with indicative values, all except Cd, which was significantly lower, aligned with Seronorm™ references.</div></div><div><h3>Conclusion</h3><div>These findings highlight the impact of plasma matrices on metal measurements in biobank studies and emphasize the importance of selecting appropriate blood matrices and collection tubes to ensure accurate and reliable elemental analysis in large-scale epidemiological research.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127667"},"PeriodicalIF":3.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Size- and month-dependent bioaccumulation of trace elements and toxic metals in atlantic bonito (Sarda sarda, Bloch, 1793): Implications for nutritional security and environmental health","authors":"Barış Bayraklı ,&nbsp;Mutlu Altuntas","doi":"10.1016/j.jtemb.2025.127664","DOIUrl":"10.1016/j.jtemb.2025.127664","url":null,"abstract":"<div><h3>Background</h3><div>Trace elements play dual roles in marine ecosystems and human health, acting as trace elements (e.g., Cu, Fe, Se, Zn) or toxic contaminants (e.g., Hg, As). This study investigates the bioaccumulation of 14 trace elements in <em>Sarda sarda</em>, a commercially and ecologically important species, to evaluate size- and season-dependent trends and their implications for environmental health and nutritional security.</div></div><div><h3>Methods</h3><div>This study investigated the bioaccumulation of heavy metals (mercury (Hg), lead (Pb), cadmium (Cd), chromium (Cr), nickel (Ni)) and trace elements (arsenic (As), zinc (Zn), iron (Fe), selenium (Se), copper (Cu), manganese (Mn), aluminum (Al), boron (B), lithium (Li)) in <em>Sarda sarda</em> individuals categorized by size classes (small, medium, large, based on body length and weight) sampled monthly from the Sinop fishery, Turkey, in September, October, and November 2023. Metal concentrations were quantified in muscle tissue using inductively coupled plasma mass spectrometry (ICP-MS) with strict quality assurance protocols. Health risk indices (EDI, THQ, TTHQ, CRR, ADC, CDR, HBVSe) were calculated to assess dietary exposure risks.</div></div><div><h3>Results size-dependent trends</h3><div>Hg and As levels increased significantly with fish size (p &lt; 0.05), reflecting trophic magnification, while Li exhibited an inverse relationship, with higher concentrations in smaller fish.</div></div><div><h3>Seasonal dynamics</h3><div>Cu, Mn, and Se peaked in September. Pb and Cd were undetectable (&lt;0.005 mg/kg), indicating low anthropogenic contamination.</div></div><div><h3>Health risks</h3><div>All metals fell below international safety thresholds.</div></div><div><h3>Conclusion</h3><div>This study highlights the dual influence of biological (fish size, trophic position) and environmental (seasonal pollutant fluxes) factors on trace element bioaccumulation in <em>Sarda sarda</em>. While current levels pose minimal health risks, proactive monitoring is essential to address emerging contaminants and ensure sustainable fisheries management. These findings provide a template for adaptive monitoring frameworks in transitional marine ecosystems.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127664"},"PeriodicalIF":3.6,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lead exposure disrupts cytoskeletal arrangement and perturbs glucose metabolism in nerve cells through activation of the RhoA/ROCK signaling pathway","authors":"Lingli Chen ,&nbsp;Siyuan An ,&nbsp;Yuye Liu ,&nbsp;Qian Jiang ,&nbsp;Yaming Ge ,&nbsp;Guoying Yu","doi":"10.1016/j.jtemb.2025.127663","DOIUrl":"10.1016/j.jtemb.2025.127663","url":null,"abstract":"<div><div>Lead (Pb) is a heavy metal environmental pollutant with strong biological toxicity. Our previous study suggested that Pb may impair learning and memory by disrupting cytoskeletal structure and inhibiting the expression of synaptic plasticity-related proteins in mice. However, the exact mechanism of Pb-induced cytoskeletal damage remains unclear. In this study, Neuro-2a cells and Kunming mice were used to explore the neurotoxic mechanism of Pb. The actin dynamics were observed via laser confocal microscopy. The ATP levels and ATPase activity in Neuro-2a cells was measured. In addition, the mRNA and protein expression levels of RhoA/ROCK/Cofilin signaling pathway in brain tissues and Neuro-2a cells was measured, and the mRNA expression levels of glucose metabolism rate-limiting enzymes were detected. Our results showed that Pb induces nerve cell damage and cytoskeletal abnormalities. Western blot and qRT-PCR analyses revealed that Pb activated the RhoA/ROCK/Cofilin signaling pathway. Additionally, ATPase activity significantly decreased following Pb treatment, whereas ATP levels markedly increased in the 50 μM Pb group. In addition, Pb disrupts brain glucose metabolism through affect the transcription of rate-limiting enzymes of glucose metabolism. Overall, these findings suggest that Pb activates the RhoA/ROCK/Cofilin signaling pathway, leading to cytoskeletal damage. Moreover, Pb exposure alters glucose metabolism enzyme activity and ATP production, disrupting the balance between F-actin and G-actin and ultimately affecting neuronal structure and function. These results may provide a better understanding of lead-induced nerve damage.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127663"},"PeriodicalIF":3.6,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress demands high dietary zinc to combat oxidative stress, and improve cytokine and immune response in rats","authors":"Eranna Lokesha ,&nbsp;Sunil Ekanath Jadhav ,&nbsp;Ganesh Narayanrao Aderao ,&nbsp;Pramod Chaudhary ,&nbsp;Kumari Priyanka ,&nbsp;Narayan Dutta ,&nbsp;Gyanendra Singh","doi":"10.1016/j.jtemb.2025.127662","DOIUrl":"10.1016/j.jtemb.2025.127662","url":null,"abstract":"<div><div>Heat stress (HS) adversely impacts livestock production, immune response, and antioxidant status. Zinc (Zn), a key component of metalloenzymes, plays a vital role in maintaining these functions, with increased Zn requirements likely under HS. This study determined the dietary Zn requirement of rats under HS. Seventy-two weaned Wistar rats were allocated into six groups in a 3 × 2 factorial design (3 Zn levels: 14.6 ppm (CON), 32.7 ppm (Zn1), and 48.9 ppm (Zn2); 2 environmental conditions: thermoneutral (TN) and HS. All groups received the same basal purified diet with varying dietary Zn supplied as zinc sulphate (ZnSO₄.7H₂O). Initially for 28 days all groups were maintained under TN condition, later three groups from each dietary Zn levels were exposed to HS (5 hr/d) for 14 days in a psychrometric chamber. Blood, serum, and organ samples were analyzed for biochemical, hormonal, immune, and mineral profiles. HS elevated stress markers (cortisol, malondialdehyde, cytokines, haptoglobin, alanine transaminase, and aspartate transaminase), while higher dietary Zn (48.9 ppm) significantly reduced these markers under HS. Antioxidant enzymes (superoxide dismutase and catalase) and humoral immune response were lower in control groups but improved with increased dietary Zn in both environments. The mineral concentrations of Zn, Cu, Mn, and Fe in serum were increased with higher dietary Zn levels in HS compared to TN groups. In conclusion, higher dietary Zn level at 48.9 ppm in a purified diet is effective in alleviating HS by lowering stress markers and bolstering antioxidant enzymes and humoral immune responses.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127662"},"PeriodicalIF":3.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-fabricated MnO₂ nanoparticles function as dual nanofertilizers and chromium remediators, enhancing antioxidant pathways, ionomic networks, and physiological resilience in wheat","authors":"Muhammad Anas ,&nbsp;Umar Masood Quraishi","doi":"10.1016/j.jtemb.2025.127661","DOIUrl":"10.1016/j.jtemb.2025.127661","url":null,"abstract":"<div><div>This study investigates the potential of green-fabricated manganese dioxide (MnO₂) nanoparticles (NPs) to mitigate chromium (Cr) toxicity in wheat, presenting a novel approach to enhancing ion homeostasis and physiological resilience under Cr stress. Chromium contamination in agricultural soils is a significant concern, severely impacting crop productivity and disrupting the physiological homeostasis of wheat. Chromium exposure compromises nutrient uptake, induces oxidative stress, and impairs plant growth and yield. This study explored the use of green-fabricated MnO₂ NPs to mitigate Cr-induced oxidative stress in two bread wheat cultivars, Borlaug-16 and SKD-1. Seed nano-priming with MnO₂ NPs (100, 250, and 500 mg kg⁻¹) was applied, followed by Cr (100 mg kg⁻¹) exposure, and key physiological, biochemical, and ionomic responses were evaluated. Manganese dioxide nanoparticles significantly reduced Cr uptake and improved ion transport. In Borlaug-16, NP250 enhanced seedling height by 74 %, while NP100 reduced H₂O₂ and TBARS by 60.28 % and 50.17 %, respectively, indicating improved oxidative stress tolerance. SKD-1 exhibited greater Cr stress tolerance, with NP250 improving root length by 31.03 % and relative water content by 56.66 %, supporting better water retention. Additionally, MnO₂ NP treatments boosted antioxidant enzyme activities, increasing APX and GPX by up to 12.47 %, and restored root and leaf anatomy, reversing Cr-induced structural damage. Furthermore, MnO₂ NPs enhanced the uptake of essential nutrients such as calcium, potassium, and magnesium, while restricting Cr translocation, improving overall nutrient efficiency. These findings emphasize the potential of MnO₂ NPs as an eco-friendly strategy for enhancing crop resilience and promoting sustainable agriculture in Cr-contaminated soils.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"89 ","pages":"Article 127661"},"PeriodicalIF":3.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}