Journal of Trace Elements in Medicine and Biology最新文献

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Targeting FOXO3a, TLR2/MyD88/NF-κB cascade, and ferroptosis by theaflavin ameliorates iron-elicited liver toxicity 黄素靶向FOXO3a、TLR2/MyD88/NF-κB级联和铁凋亡可改善铁诱导的肝毒性。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-16 DOI: 10.1016/j.jtemb.2025.127758
Afnan Bakhsh , Samir A. Salama , Musaad M. Althobaiti , Shuruq E. Alsufyani , Abdullah M. Almalki , Samyah T. Alanazi
{"title":"Targeting FOXO3a, TLR2/MyD88/NF-κB cascade, and ferroptosis by theaflavin ameliorates iron-elicited liver toxicity","authors":"Afnan Bakhsh ,&nbsp;Samir A. Salama ,&nbsp;Musaad M. Althobaiti ,&nbsp;Shuruq E. Alsufyani ,&nbsp;Abdullah M. Almalki ,&nbsp;Samyah T. Alanazi","doi":"10.1016/j.jtemb.2025.127758","DOIUrl":"10.1016/j.jtemb.2025.127758","url":null,"abstract":"<div><h3>Background</h3><div>While iron is essential in trace amounts, elevated level represents a serious health problem. Elevated iron level arises as a secondary consequence to hemochromatosis and anemias that necessitate frequent blood transfusions, leading to organ toxicity, particularly liver toxicity.</div></div><div><h3>Methods</h3><div>The current study investigated the potential ameliorating impact of theaflavin against the iron-elicited liver toxicity. A model of iron intoxication was established in male Wistar rats, and theaflavin was given over a 10-day period. Blood and liver specimens were collected and subjected to histopathological, ELISA, biochemical, and Western blotting investigations.</div></div><div><h3>Results</h3><div>Theaflavin suppressed the iron-evoked liver injury as indicated by a significant decrease in activity of the hepatocellular enzymes in sera and improved hepatic histopathological architecture. Theaflavin activated the antioxidant transcription factor FOXO3a with upregulation of its responsive antioxidant gene products including thioredoxin reductase, superoxide dismutase, and catalase, along with reduced DNA oxidative modification. Equally important, theaflavin suppressed TLR2 inflammatory cascade as evidenced by a significant downregulation in protein expression of TLR2 and its adaptor protein MyD88, and inhibition of phosphorylation and nuclear translocation of its downstream inflammatory transcription factor NF-κB. In the same context, theaflavin markedly reduced levels of NF-κB-responsive cytokines TNF-α and IL-6. Interestingly, theaflavin repressed the iron-elicited hepatocellular ferroptosis as indicated by modulation of its biomarkers GPx4 and COX-2 protein expression, and levels of lipid hydroperoxides and hepatocellular iron load.</div></div><div><h3>Conclusion</h3><div>These findings emphasize the ameliorating impact of theaflavin against the iron-elicited liver toxicity and shed light on FOXO3a, TLR2/MyD88/NF-κB cascade, and ferroptosis as possible molecular targets.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127758"},"PeriodicalIF":3.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Liraglutide orchestrates ferroptosis defense against murine cisplatin acute kidney injury: NRF2 activation via both KEAP1-dependent and -independent mechanisms is essential for SLC7A11/GPX4 renoprotection 利拉鲁肽协调铁凋亡防御小鼠顺铂急性肾损伤:通过keap1依赖性和非依赖性机制激活NRF2对于SLC7A11/GPX4肾保护至关重要。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-16 DOI: 10.1016/j.jtemb.2025.127755
Nermeen S. Abdel Razek , Noha N. Nassar , Rabab H. Sayed , Ayman E. El-Sahar , Dalaal M. Abdallah
{"title":"Liraglutide orchestrates ferroptosis defense against murine cisplatin acute kidney injury: NRF2 activation via both KEAP1-dependent and -independent mechanisms is essential for SLC7A11/GPX4 renoprotection","authors":"Nermeen S. Abdel Razek ,&nbsp;Noha N. Nassar ,&nbsp;Rabab H. Sayed ,&nbsp;Ayman E. El-Sahar ,&nbsp;Dalaal M. Abdallah","doi":"10.1016/j.jtemb.2025.127755","DOIUrl":"10.1016/j.jtemb.2025.127755","url":null,"abstract":"<div><h3>Objective</h3><div>Acute kidney injury (AKI) induced by oxidative stress, and recently associated with ferroptosis, represents a major complication of the chemotherapeutic cisplatin that often necessitates treatment cessation. The glucagon-like peptide 1 receptor (GLP1R) agonist liraglutide possesses reno-protective potential via its antioxidant character in different kidney injury settings while reducing iron overload in other models. Hence, we investigated the potential protective role of liraglutide in cisplatin-induced AKI targeting KEAP1-dependent and -independent ferroptosis pathways.</div></div><div><h3>Methods</h3><div>Rats were assigned to one of four groups: vehicle control, liraglutide control, cisplatin-induced AKI, and liraglutide-pretreated AKI. Renal function markers and histopathological changes were assessed. SLC7A11, NRF2, and KEAP1-canonical and non-canonical hubs were analyzed to elucidate the drug’s molecular mechanisms on ferroptosis.</div></div><div><h3>Results</h3><div>Liraglutide significantly improved renal function, evidenced by the reduction of serum cystatin C, creatinine, and BUN, along with renal histological improvements. In the kidney, liraglutide activated/phosphorylated AKT, mTOR, and P62 to reduce KEAP1 and inactivated GSK3β to enhance NRF2-mediated GPX4 and SLC7A11 formation, thus inhibiting cisplatin-ferroptosis-triggered renal injury.</div></div><div><h3>Conclusion</h3><div>Therefore, liraglutide is a promising treatment candidate for attenuating cisplatin-induced AKI by SLC7A11/GPX4 trajectory through upregulating the AKT/mTOR/P62/KEAP1/NRF2 and AKT/GSK3β/NRF2 signaling pathways to increase GPX4 alongside with SLC7A11. Indeed, the GLP1R-mediated AKT activation acts as a potential target for Liraglutide reno-protective actions; hence, the GLP1R can be considered a therapeutic entity in such a renal injurious paradigm.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127755"},"PeriodicalIF":3.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neuroprotective efficacy of royal jelly and propolis against cadmium-induced dysregulation of neurogenesis and neurotransmitters in the brain of rats: Molecular mechanisms and ultrastructure investigations 蜂王浆和蜂胶对镉诱导的大鼠脑神经发生和神经递质失调的神经保护作用:分子机制和超微结构研究。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-16 DOI: 10.1016/j.jtemb.2025.127756
Norhan S. El-Sayed , Eman M. Omar , Esraa S. Habiba , Sahar A. Harby , Amany Attaallah , Maria Augustyniak , Abeer El Wakil , Lamia M. El-Samad , Mohamed A. Hassan
{"title":"Neuroprotective efficacy of royal jelly and propolis against cadmium-induced dysregulation of neurogenesis and neurotransmitters in the brain of rats: Molecular mechanisms and ultrastructure investigations","authors":"Norhan S. El-Sayed ,&nbsp;Eman M. Omar ,&nbsp;Esraa S. Habiba ,&nbsp;Sahar A. Harby ,&nbsp;Amany Attaallah ,&nbsp;Maria Augustyniak ,&nbsp;Abeer El Wakil ,&nbsp;Lamia M. El-Samad ,&nbsp;Mohamed A. Hassan","doi":"10.1016/j.jtemb.2025.127756","DOIUrl":"10.1016/j.jtemb.2025.127756","url":null,"abstract":"<div><div>This study seeks to evaluate the neuroprotective efficacy of royal jelly (RJ) and propolis (P) against cadmium-induced dysregulation of neurogenesis and neurotransmitters in the brains of <em>Wistar</em> male rats and decipher implicated underlying mechanisms. We probed oxidative stress, inflammatory, neurotransmitter, and neurogenesis biomarkers, along with histopathological and ultrastructural attributes. Energy dispersive x-ray (EDX) microanalysis revealed that RJ and P administration diminished the cadmium (Cd) ratio in the brain compared with the Cd-administered rats. Accordingly, Cd triggered a noticeable disturbance in recognition, which was counterbalanced by RJ and P therapies. Additionally, Cd-administered rats demonstrated lessened superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione S-transferase (GST) activities with increased malondialdehyde (MDA) level. Furthermore, Cd treatment led to substantial rises in nuclear factor kappa B (NF-κB), Indoleamine 2,3-dioxygenase (IDO), and tumor necrosis factor alpha (TNF-α) concentrations, with a dwindled tissue inhibitor of metalloproteinase 3 (TIMP3) level. These deregulations were significantly rectified by RJ and P therapies. Crucially, Cd accumulation disrupted levels of neurotransmitters, including dopamine (DA), serotonin (SRO), and amyloid beta 1–42 (Aβ1–42), and acetylcholinesterase (AChE) activity, along with a notable diminution in neurogenesis biomarkers, involving brain-derived neurotrophic factor (BDNF) level and doublecortin (DCX) mRNA expression. Notably, RJ and P counteracted these interferences and reinstated levels of major factors to those in the control group. Interestingly, glial fibrillary acidic protein (GFAP) immunohistochemistry, histological, and ultrastructure investigations disclosed salient correlations with biochemical findings. Altogether, our results substantiate the effective and better utilization of RJ than P to counterbalance the pernicious Cd consequences.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127756"},"PeriodicalIF":3.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145202500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heteroleptic N, N donor carbonitrile based Au(III) complexes: DNA/BSA interaction studies, Molecular Docking, and cytotoxicity studies 异电性N, N供体碳腈基Au(III)配合物:DNA/BSA相互作用研究,分子对接和细胞毒性研究
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-16 DOI: 10.1016/j.jtemb.2025.127747
Nikita J. Patel , Ravi A. Dabhi , Milan P. Dhaduk , Nirbhay K. Savaliya , Bhupesh S. Bhatt , Miral V. Lunagariya , Vaibhav D. Bhatt , Mohan N. Patel
{"title":"Heteroleptic N, N donor carbonitrile based Au(III) complexes: DNA/BSA interaction studies, Molecular Docking, and cytotoxicity studies","authors":"Nikita J. Patel ,&nbsp;Ravi A. Dabhi ,&nbsp;Milan P. Dhaduk ,&nbsp;Nirbhay K. Savaliya ,&nbsp;Bhupesh S. Bhatt ,&nbsp;Miral V. Lunagariya ,&nbsp;Vaibhav D. Bhatt ,&nbsp;Mohan N. Patel","doi":"10.1016/j.jtemb.2025.127747","DOIUrl":"10.1016/j.jtemb.2025.127747","url":null,"abstract":"<div><div>A series of square planar substituted carbonitrile based Au(III) complexes (2a-2e) with the type of [(N^N)Au(Cl<sub>2</sub>)]<sup>+</sup>Cl<sup>-</sup> [Where, N^N = 5- Amino-3-phenyl-1-(pyridin-2-yl)-1H-pyrazole-4-carbonitrile (1a), 5-amino-3-(4-chlorophenyl)-1-(pyridin-2-yl)-1H-pyrazole-4-carbonitrile (1b), 5-amino-3-(4-bromophenyl)-1-(pyridin-2-yl)-1H-pyrazole-4-carbonitrile (1c), 5-amino-3-(4-flourophenyl)-1-(pyridin-2-yl)-1H-pyrazole-4-carbonitrile (1d), 5-amino-3-(4-methoxyphenyl)-1-(pyridin-2-yl)-1H-pyrazole-4-carbonitrile (1e) were synthesized and characterized by analytical techniques. UV-Vis spectroscopy, fluorescence spectroscopy, Viscosity measurements, and molecular docking studies were used to identify the binding mechanism between complexes and HS-DNA (Herring sperm-DNA), results suggest an intercalation mode of DNA binding. Protein binding studies of the complexes were evaluated using UV–visible and fluorescence spectroscopy. The antibacterial activity of the metal chelates were performed against three Gram (-ve) and two Gram (+ve) bacteria, and the results indicate that all of the complexes perform better than their ligands against microorganisms. The <em>in vitro</em> cytotoxicity against BSLB (Brine shrimp lethality bioassay) of ligands and metal chelates were also carried out. The LC<sub>50</sub> values of the ligands and complexes were found in the range of 10.45–13.28 µg/mL and 6.30–6.99 µg/mL, respectively. All compounds were tested for anti-cancer activity against the human adenocarcinoma (MCF-7) cancer cell line. The results show that effective toxicity against the tested cell line of all metal chelates. The SwissADME webserver analysis confirmed that most of the synthetic compounds comply with drug-likeness principles. Additionally, they demonstrated promising biological activities, indicating their potential for further drug development.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127747"},"PeriodicalIF":3.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145097747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serum trace elements and expression of antioxidant-related genes in the detection of women with ER-positive breast cancer 血清微量元素及抗氧化相关基因表达在er阳性乳腺癌检测中的意义
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-13 DOI: 10.1016/j.jtemb.2025.127752
Paula Argente del Castillo , Pere Miquel Morla-Barcelo , Toni Martinez-Bernabe , Sara Sánchez Asís , Jose Antonio Delgado Rodríguez , Mercedes Nadal-Serrano , Isabel Llompart Alabern , Pilar Roca , Octavi Cordoba , Jorge Sastre-Serra , Josep Miquel Bauça
{"title":"Serum trace elements and expression of antioxidant-related genes in the detection of women with ER-positive breast cancer","authors":"Paula Argente del Castillo ,&nbsp;Pere Miquel Morla-Barcelo ,&nbsp;Toni Martinez-Bernabe ,&nbsp;Sara Sánchez Asís ,&nbsp;Jose Antonio Delgado Rodríguez ,&nbsp;Mercedes Nadal-Serrano ,&nbsp;Isabel Llompart Alabern ,&nbsp;Pilar Roca ,&nbsp;Octavi Cordoba ,&nbsp;Jorge Sastre-Serra ,&nbsp;Josep Miquel Bauça","doi":"10.1016/j.jtemb.2025.127752","DOIUrl":"10.1016/j.jtemb.2025.127752","url":null,"abstract":"<div><h3>Background</h3><div>Breast cancer is the most frequently diagnosed malignancy among women worldwide. Trace elements, such as selenium, play a crucial role in antioxidation and have gained interest in cancer research. This study aimed to assess the association between prediagnostic serum trace element concentrations and breast cancer risk, as well as their relationship with the expression of key antioxidant enzymes in leukocytes of ER-positive breast cancer patients.</div></div><div><h3>Methodology</h3><div>A cross-sectional study compared breast cancer patients to cancer-free individuals. Serum samples were analyzed for zinc, selenium, and copper concentrations, while leukocytes were isolated for the quantification of antioxidant-related gene expression, including SOD1, SOD2, PRDX2, PRDX5, PRDX6, TXN, UCP2, and UCP5. The predictive diagnostic capacity of identified trace elements and genes was assessed using receiver operating characteristic (ROC) curves, and correlations between gene expression and patient prognosis were evaluated. Results: Breast cancer patients had lower serum selenium and zinc levels and reduced PRDX2, PRDX5, and UCP2 mRNA expression in leukocytes, whereas TXN, UCP5, and SOD2 were upregulated. Two signatures based on potential trace elements and genes showed high predictive accuracy (AUC = 0.97 and 0.98, respectively). Moreover, upregulated genes correlated with ER-positive tumors associated with poor prognosis.</div></div><div><h3>Conclusions</h3><div>These mRNA expression patterns and bioinformatic approaches, in combination with clinical variables and trace elements plasma levels, could give an approximation to create a signature to better understand the progression or aggressiveness of ER-positive breast cancer with a simple and cost-effective blood test.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127752"},"PeriodicalIF":3.6,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of blueberries on non-heme iron absorption in adult women 蓝莓对成年女性非血红素铁吸收的影响。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-13 DOI: 10.1016/j.jtemb.2025.127753
Gabriela Galán-Ramírez , Daniela Moya , Manuel Olivares , Lee A. Meisel , Miguel Arredondo , Fernando Pizarro
{"title":"Effect of blueberries on non-heme iron absorption in adult women","authors":"Gabriela Galán-Ramírez ,&nbsp;Daniela Moya ,&nbsp;Manuel Olivares ,&nbsp;Lee A. Meisel ,&nbsp;Miguel Arredondo ,&nbsp;Fernando Pizarro","doi":"10.1016/j.jtemb.2025.127753","DOIUrl":"10.1016/j.jtemb.2025.127753","url":null,"abstract":"<div><div>Iron deficiency, the leading cause of anemia, can be exacerbated by dietary inhibitors such as polyphenols in fruits and vegetables. Despite their high polyphenol content, blueberries contain antioxidant compounds such as ascorbic acid and carotenoids, which are known to enhance iron absorption. This study aims to assess the net effect of blueberry consumption on non-heme iron absorption in healthy, non-menopausal women. Fourteen women aged 38–50 years, with contraception and a negative pregnancy test, participated in the study. After overnight fasting, the subjects were administered iron with or without fresh blueberries on four different intervention days. On day 1, the subjects received FeSO<sub>4</sub> solution as control (C); on day 2, FeSO<sub>4</sub> + 100 g of blueberry (B); on day 14, FeSO<sub>4</sub> + 100 g of blueberry + standardized breakfast (A); and on day 15, FeSO<sub>4 +</sub> breakfast (D). All servings contained 5 mg of Fe labeled with radioactive isotopes (⁵⁵Fe, ⁵⁹Fe). Blood samples were taken on days 14 and 28 to measure the iron bioavailability and nutritional status. The iron bioavailability in the different groups was (geometric mean and range ±1 SD): C= 30.2 % (13.8–66.0 %), B= 6.8 % (2.1–21.7 %), A= 7.5 % (2.3–24.0 %), and D= 5.7 % (1.5–22.4 %); ANOVA for repeated measures F= 10.2 p &lt; 0.0001 (Dunnett post hoc test: C vs. A, B, and D, p &lt; 0.0001). In conclusion, this study found that fresh blueberries, with or without breakfast, significantly decreased non-heme iron absorption. Given the small sample size and the limited population studied, these findings should be considered preliminary and warrant confirmation in larger studies.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127753"},"PeriodicalIF":3.6,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heavy metals and trace elements in a threatened population of guanay cormorants (Leucocarbo bougainvilliorum) from an industrialized bay in the Humboldt Current System, Chile 智利洪堡洋流系统工业化海湾中受威胁的鸬鹚(Leucocarbo bougainvilliorum)的重金属和微量元素。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-12 DOI: 10.1016/j.jtemb.2025.127749
Jorge Carretero , Ana M. García-Cegarra , Emma Martínez-López
{"title":"Heavy metals and trace elements in a threatened population of guanay cormorants (Leucocarbo bougainvilliorum) from an industrialized bay in the Humboldt Current System, Chile","authors":"Jorge Carretero ,&nbsp;Ana M. García-Cegarra ,&nbsp;Emma Martínez-López","doi":"10.1016/j.jtemb.2025.127749","DOIUrl":"10.1016/j.jtemb.2025.127749","url":null,"abstract":"<div><div>Metal contamination from industrial and mining activities threatens coastal and marine ecosystems, leading to bioaccumulation in marine organisms and biomagnification through the trophic chain. The Guanay cormorant (<em>Leucocarbo bougainvilliorum</em>), an endemic species of the Humboldt Current System, is experiencing population declines and serves as a key bioindicator of heavy metal contamination due to its high trophic position, reliance on commercially exploited pelagic fish, and capacity to accumulate contaminants over time. This study analyzed the concentrations of five heavy metals (Hg, As, Pb, Cr, Cd) and three trace elements (Cu, Zn, Se) in liver, muscle, feathers, and kidney of 10 Guanay cormorants found dead in Mejillones Bay, Chile, a highly industrialized area. Feathers exhibited the highest concentrations of Cr (2.03 ± 0.63 mg/kg), Cu (19.72 ± 3.82 mg/kg), and Zn (109.84 ± 11.95 mg/kg), emphasizing their role in detoxification. Females showed significantly higher Cu and Pb concentrations in muscle and Cr in kidneys, likely due to physiological and hormonal differences related to reproduction. Correlations between metal concentrations across tissues suggest shared bioaccumulation mechanisms, while the Hg:Se molar ratio (e.g., 0.25 ± 0.04 in liver) indicated a protective role of Se against Hg toxicity. Metal concentrations were comparable to those reported in other marine cormorants but differed from freshwater populations, highlighting the influence of habitat on metal accumulation. Although current levels do not indicate acute toxicity, the ongoing population decline of Guanay cormorants underscores the need for long-term biomonitoring. Future research incorporating biochemical and enzymatic biomarkers is essential to assess potential sublethal effects and long-term ecological risks associated with heavy metal exposure in this species.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127749"},"PeriodicalIF":3.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Zinc and circulating microRNAs in women with insulin resistance 锌和循环microrna在女性胰岛素抵抗中的作用。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-12 DOI: 10.1016/j.jtemb.2025.127748
Danielle Caroline da Silva Dias , Graziela Biude Silva Duarte , Leonam da Silva Pereira Batista , Marcelo Macedo Rogero , Fernando Barbosa Jr. , Silvia Maria Franciscato Cozzolino , Raquel Costa Silva Dantas-Komatsu , Karina Zaira Silva Marinho Costa , Bruna Zavarize Reis
{"title":"Zinc and circulating microRNAs in women with insulin resistance","authors":"Danielle Caroline da Silva Dias ,&nbsp;Graziela Biude Silva Duarte ,&nbsp;Leonam da Silva Pereira Batista ,&nbsp;Marcelo Macedo Rogero ,&nbsp;Fernando Barbosa Jr. ,&nbsp;Silvia Maria Franciscato Cozzolino ,&nbsp;Raquel Costa Silva Dantas-Komatsu ,&nbsp;Karina Zaira Silva Marinho Costa ,&nbsp;Bruna Zavarize Reis","doi":"10.1016/j.jtemb.2025.127748","DOIUrl":"10.1016/j.jtemb.2025.127748","url":null,"abstract":"<div><h3>Introduction</h3><div>Insulin resistance (IR) is a key factor in the development of type 2 diabetes and is potentially associated with zinc deficiency due to the role of this mineral in the metabolism of this hormone. MicroRNAs (miRs) can regulate insulin signaling pathway, glucose metabolism, and inflammation, and their expression can be modulated by different nutrients and bioactive compounds. Nutrimiromics investigates how nutrients modulate gene expression through miRs. However, the associations between miRNAs related to IR mechanisms and zinc status in the body remains unclear. Therefore, this study aimed to evaluate the associations of plasma zinc concentration with the expression of miR-191–5p, miR-188–5p, miR-145–5p, and miR-143–3p in overweight and IR women<strong>.</strong></div></div><div><h3>Materials and methods</h3><div>This comparative cross-sectional study included 50 overweight or obese women, aged between 18 and 60 years. IR classification followed Stern's criteria. Circulating miR expression, plasma zinc concentration, biochemical profile, inflammatory biomarkers, and anthropometric parameters were evaluated.</div></div><div><h3>Results</h3><div>Six percent of the participants had plasma zinc concentrations below the reference value (&lt;70 µg/dL). When categorizing plasma zinc values by their median concentration (≤90 µg/dL and &gt;90 µg/dL), a significant difference was observed in the fibrinogen concentration (p = 0.016) and the expression of three miR evaluated (miR-191–5p, miR-145–5p, and miR-143–3p). A significant inverse correlation was found between plasma zinc concentration and miR-145–5p (p = 0.006), miR-143–3p (p = 0.011), miR-191–5p (p = 0.002), and the serum fibrinogen concentration (p = 0.038). Significant inverse correlations were observed between insulin and miR-191–5p (p = 0.007), miR-188–5p (p = 0.029), miR-145–5p (p = 0.015), and miR-143–3p (p = 0.001). miR-191–5p (p = 0.037) and miR-143–3p (p = 0.021) were inversely correlated with HOMA-IR. The miR-145–5p was negatively correlated with IL-1β (p = 0.006), IL-6 (p = 0.004), and IFN-γ (p = 0.020), and miR-143–3p was negatively correlated with IL-6 (p = 0.018). According to the adjusted logistic regression model, high expression of miR-191–5p (OR=0.06; p = 0.012) was inversely associated with the plasma zinc concentration. In the functional enrichment analysis conducted with the list of target genes, six Gene Ontology (GO) terms were significantly enriched.</div></div><div><h3>Conclusion</h3><div>All four miRs showed an inverse correlation with insulin, indicating that zinc status may influence miR expression, potentially affecting biological processes involved in IR. This study identified significant expression of miR-143–3p, miR-145–5p, and miR-191–5p at plasma zinc concentrations above 90 μg/dL.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127748"},"PeriodicalIF":3.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hexavalent chromium exposure impacts the metabolome of human lung fibroblast cells 六价铬暴露对人肺成纤维细胞代谢组的影响。
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-12 DOI: 10.1016/j.jtemb.2025.127750
James T.F. Wise , Davide Chiarugi , Jinhua Chi , John Pierce Wise Sr.
{"title":"Hexavalent chromium exposure impacts the metabolome of human lung fibroblast cells","authors":"James T.F. Wise ,&nbsp;Davide Chiarugi ,&nbsp;Jinhua Chi ,&nbsp;John Pierce Wise Sr.","doi":"10.1016/j.jtemb.2025.127750","DOIUrl":"10.1016/j.jtemb.2025.127750","url":null,"abstract":"<div><h3>Introduction</h3><div>Lung cancer is the number one cause of cancer death and survival rates have not significantly improved for decades. The underlying mechanism of lung cancer remains elusive, but it is known chromosome instability, altered DNA, and dysregulated cellular energetics are key to cancers. However, the importance of energetics during chemical carcinogenesis is unexplored.</div></div><div><h3>Methods</h3><div>We exposed human lung cells to hexavalent chromium [Cr(VI)], a known human respiratory carcinogen, for 24 or 120 h. Then, we performed untargeted metabolomics and analyzed changes with concentration and time through differential metabolomics.</div></div><div><h3>Results</h3><div>There was a distinct metabolic response with increasing Cr(VI) concentrations and a difference in the metabolic response with time, where metabolic enrichment of amino acid metabolism and nucleotide metabolism were affected with time and Cr(VI) treatment.</div></div><div><h3>Conclusions</h3><div>This study provides the first link between energy metabolism responses and chemical carcinogenesis, providing the foundation for a more in-depth investigation into how Cr(VI) alters the metabolic response of cells to induce lung cancer. We anticipate our study will be a starting point for more in-depth investigations into the role of dysregulated cellular energetics during carcinogenesis, which will further establish these changes as a critical early-stage event in carcinogenesis.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127750"},"PeriodicalIF":3.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145082896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metallothionein 2A alleviates ulcerative colitis by inhibiting ferroptosis in intestinal epithelial cells with Tfrc downregulation 金属硫蛋白2A通过抑制Tfrc下调的肠上皮细胞铁下垂来缓解溃疡性结肠炎
IF 3.6 3区 医学
Journal of Trace Elements in Medicine and Biology Pub Date : 2025-09-10 DOI: 10.1016/j.jtemb.2025.127746
Siqi Hua , Shuangshuang Song , Chunhao Yang , Jingwen Xu , Zhonghua Li , Bo Zhu , Zichun Hua
{"title":"Metallothionein 2A alleviates ulcerative colitis by inhibiting ferroptosis in intestinal epithelial cells with Tfrc downregulation","authors":"Siqi Hua ,&nbsp;Shuangshuang Song ,&nbsp;Chunhao Yang ,&nbsp;Jingwen Xu ,&nbsp;Zhonghua Li ,&nbsp;Bo Zhu ,&nbsp;Zichun Hua","doi":"10.1016/j.jtemb.2025.127746","DOIUrl":"10.1016/j.jtemb.2025.127746","url":null,"abstract":"<div><h3>Background</h3><div>Ferroptosis has emerged as a critical pathogenic mechanism in ulcerative colitis (UC). Metallothioneins (MTs), known for their redox regulatory functions, may protect against ferroptosis in UC. However, the underlying mechanisms are still unclear.</div></div><div><h3>Methods</h3><div>We conducted comprehensive investigations combining a bioinformatics analysis of a human UC dataset (GSE243625) with experimental validations using DSS-induced murine colitis models and RSL3-treated HCT116 human colon epithelial cells. Our experimental approaches included immunohistochemical analysis of MT2 expression, assessment of ferroptosis markers, evaluation of mitochondrial function, measurement of mitochondrial redox status and Fe<sup>2+</sup>, and profiling of protein expression. The therapeutic potential of ZnO nanoparticles was evaluated <em>in vivo</em>.</div></div><div><h3>Results</h3><div>Transcriptomic analysis revealed significant downregulation of all nine MT family members in UC patients, with the most pronounced suppression occurring in active disease. This finding was corroborated in murine models, which showed reduced colonic MT2 expression following DSS treatment. Cellular studies demonstrated that MT2A overexpression provides robust protection against RSL3-induced ferroptosis, evidenced by a reduction in lipid peroxidation, preservation of mitochondrial integrity, and decreased accumulation of mitochondrial reactive oxygen species. Mechanistically, MT2A modulated cellular iron homeostasis by downregulating transferrin receptor (Tfrc). Importantly, the oral administration of ZnO nanoparticles effectively restored MT2 expression, downregulated Tfrc, attenuated ferroptosis, and significantly alleviated colitis symptoms in vivo.</div></div><div><h3>Conclusions</h3><div>Our findings establish MT2A as a crucial regulator of ferroptosis in UC pathogenesis and identify ZnO nanoparticle-mediated MT2 induction and Tfrc downregulation as a promising therapeutic strategy. This study provides novel insights into the MT2A-ferroptosis axis and its potential translational applications for UC treatment.</div></div>","PeriodicalId":49970,"journal":{"name":"Journal of Trace Elements in Medicine and Biology","volume":"92 ","pages":"Article 127746"},"PeriodicalIF":3.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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