Luminescence最新文献

{"title":"Correction to “Bioluminescent Eddies of the World Ocean”","authors":"","doi":"10.1002/bio.70121","DOIUrl":"https://doi.org/10.1002/bio.70121","url":null,"abstract":"<p>\u0000 <span>S. A. Piontkovski</span>, <span>A. V. Melnik</span>, <span>I. M. Serikova</span>, <span>I. A. Minsky</span>, <span>V. F. Zhuk</span>, <i>Luminescence</i> <span>2023</span>, <span>38</span>(<span>4</span>), <span>505</span>, https://doi.org/10.1002/bio.4475.\u0000 </p><p>Reference 21 is incorrect. It should be as follows:</p><p>21. Yu. V. Artamonov, E. A. Skripaleva, A. V. Fedirko, S. А. Shutov, D. V. Derjushkin, R. O. Shapovalov, Yu. I. Shapovalov, S. V. Shcherbachenko, Ecological Safety of Coastal and Shelf Zones of Sea, 2020, 1, 69 (in Russian). doi:10.22449/2413-5577-2020-1-69–90</p><p>We apologize for this error.</p>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/bio.70121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143475436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoluminescence Properties of Novel Ca3Y2B4O12:Eu3+ and Dy3+ Phosphors for Solid-State Lighting Prepared by Combustion Method","authors":"C. M. Nandanwar,&nbsp;N. S. Kokode,&nbsp;A. N. Yerpude,&nbsp;A. M. Uke","doi":"10.1002/bio.70126","DOIUrl":"https://doi.org/10.1002/bio.70126","url":null,"abstract":"<p>In this study, novel Ca₃Y₂B₄O₁₂:Eu³⁺ and Dy³⁺ phosphors were synthesized utilizing a simple and efficient combustion process. The Ca₃Y₂B₄O₁₂ host orthorhombic phase with high crystallinity formation is confirmed by structural x-ray diffraction analysis. The Ca₃Y₂B₄O₁₂:Eu³⁺ phosphors PL study indicated different emission peaks at 595 and 618 nm under 397 nm excitation, whereas Ca₃Y₂B₄O₁₂:Dy³⁺ phosphors emission peaks displayed blue (485 nm) and yellow (579 nm) under 353 nm excitation. The ideal doping concentration of 0.5 mol% for Eu³⁺ and Dy³⁺ ions resulted in maximum emission intensity due to reduced concentration quenching effects. The chromaticity coordinates show that the emissions are in the visible spectrum, with high-purity colors suitable for solid state lighting. The results indicate that novel Ca₃Y₂B₄O₁₂:Eu³⁺ and Dy³⁺ is promising materials for n-UV emitting phosphors for solid-state lighting applications.</p>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143455735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bi4Ti3O12 Crystallized in 40Bi2O3-40B2O3-20TiO2 Glass Matrix for Photo-Piezocatalytic Dye Degradation","authors":"Ashis Kumar Moharana,&nbsp;Akshay Gaur,&nbsp;Rahul Vaish","doi":"10.1002/bio.70124","DOIUrl":"https://doi.org/10.1002/bio.70124","url":null,"abstract":"<div>\u0000 \u0000 <p>The coupling of photocatalysis and piezocatalysis has gained significant interest among researchers. In this study, we present the photo-piezocatalytic activity exhibited by bismuth titanate (Bi₄Ti₃O₁₂) glass-ceramics (BTO-GC). BTO-GC samples were prepared in a glass system of 40Bi₂O₃-40B₂O₃-20TiO₂ (in mol %) by using the melt-quench technique. The catalyst was characterized by the X-ray diffraction (XRD) technique and Raman spectroscopy for its structure and phase analysis. Surface morphology and chemical composition were analyzed by scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS), respectively. The catalytic performance of BTO-GC was evaluated in photocatalysis, piezocatalysis, and photo-piezocatalysis processes. Methylene blue (MB) dye degradation was evaluated by using a UV–Vis spectrophotometer. A maximum degradation performance of ~86% was achieved in the solar light photocatalysis process. This study highlights the synergistic photo-piezocatalytic performance in glass-ceramics and advances the understanding of its underlying mechanism.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Copper Nanoparticles Using Panchagavya: Nanomaterials for Antibacterial, Anticancer, and Environmental Applications","authors":"Samuthirapandi Muniasamy,&nbsp;Marissa Angelina,&nbsp;Ponnirul Ponmanickam,&nbsp;Mysoon M. Al-Ansari,&nbsp;Antony Mythili,&nbsp;Saurav Dixit,&nbsp;Krishnan Raguvaran","doi":"10.1002/bio.70117","DOIUrl":"https://doi.org/10.1002/bio.70117","url":null,"abstract":"<div>\u0000 \u0000 <p>This study explores the green synthesis of copper nanoparticles (Cu-NPs) using Panchagavya, a traditional organic substance composed of five cow-derived components, as a reducing and stabilizing agent. Cu-NPs were characterized using UV–Vis, FT-IR, XRD, SEM-EDS, TEM, DLS, and zeta potential analysis, which revealed their size, shape, and elemental properties. They exhibited strong antibacterial activity against <i>Bacillus cereus</i> and <i>Pseudomonas aeruginosa</i>. Biofilm inhibition was observed at various concentrations, with 38.98% at ½ MIC, 67.48% at MIC, and 84.03% at 2× MIC. SEM analysis confirmed that Cu-NPs disrupted the bacterial cell membrane, causing leakage of cellular contents. Antioxidant assays (DPPH, FRAP) revealed high scavenging activity, with percentages of 88.50% and 92.54%, respectively. Cu-NPs showed anticancer activity on MCF7 cells, with an IC₅₀ of 38.18 μg/mL. Additionally, Cu-NPs significantly reduced nitric oxide (NO) production in RAW 264.7 macrophage cells in a dose-dependent manner. The Cu-NPs also exhibited larvicidal efficacy, with 99.12% mortality against <i>Aedes aegypti</i> and 95.26% against <i>Culex quinquefasciatus</i>, and LC₅₀ values of 29.40 μg/mL and 93.55 μg/mL, respectively. Morphological changes in treated larvae included body shrinkage and degeneration of tracheal tube and ventral brush were noticed as compared to control. Histopathological examinations of Cu-NP-treated larvae showed several structural damages, including damage to gut epithelial cells, dissipation of the muscle layer, and loss of goblet cells. GC–MS analysis of Panchagavya revealed its potential for various biological applications. These findings highlight the eco-friendly and multifunctional nature of Panchagavya-mediated Cu-NPs, demonstrating their potential for antimicrobial, antioxidant, anticancer, and larvicidal applications, which could contribute to sustainable pest and disease management strategies.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile Incorporation of the Trioxosulfate Ion Preservative as a Novel Sulfonated Isoindole Fluorescent Marker for Sustainable Determination of an Antidiabetic Alogliptin Drug; Method's Sustainability Evaluation","authors":"Badriah Saad Al-Farhan,&nbsp;Safaa F. Saleh,&nbsp;Afnan S. Batubara,&nbsp;Dalin A. Hassan,&nbsp;Nujud Maslamani,&nbsp;Deena Mohammed Bukhary,&nbsp;Ahmed Abdulhafez Hamad","doi":"10.1002/bio.70100","DOIUrl":"https://doi.org/10.1002/bio.70100","url":null,"abstract":"<div>\u0000 \u0000 <p>The proposed study uses a special procedure to verify that an analyte assessment operation is sustainable and environmentally friendly. When the targeted analyte molecule (alogliptin drug, ALT) lacks chromophoric or fluorogenic groups, it cannot be analyzed without marking or structural amendment. The obeyed phenomenon (marking process) could be achieved via fluorescence probing or marking using tagging agents. An innovative reagent pair consisting of trioxosulfate ion and <i>o</i>-Phthaldialdehyde (OPD) is used in the described analytical approach. This reagent is used to build a novel fluorescent nucleus and can be detected using the developed fluorimetric approach. ALT's amino moiety could condense trioxosulfate/OPD. This study presents a new, facile, green, sustainable fluorometric assay of the ALT drug. The product's response is linear at 50.00–800.00 ng mL⁻¹ concentration range (LOD = 15.74 and LOQ = 47.69 ng mL⁻¹). The method validation was considered following the International Council for Harmonisation (ICH) recommendations. The planned probe was applied to the crude powder and dose forms, and excipients had no influence. The planned analytical procedure is identified as sustainable and white by the WAC (White Analytical Chemistry) indications following the Green/Red/Blue (RGB 12) pattern. Additionally, new tools (BAGI and GSST) were applied to the current work.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectacular Application of Isoindole-Based Fluorophore for Mirabegron Assay in Tablets and Biological Fluids (Urine/Plasma). Assessment of Method Blueness and Whiteness","authors":"Ahmed A. Abu-hassan,&nbsp;Wael A. Mahdi,&nbsp;Sultan Alshehri,&nbsp;Bandar R. Alsehli,&nbsp;Safaa F. Saleh,&nbsp;Mohamed A. El Hamd","doi":"10.1002/bio.70120","DOIUrl":"https://doi.org/10.1002/bio.70120","url":null,"abstract":"<div>\u0000 \u0000 <p>The FDA has approved mirabegron (MBG) for alleviating urgency in micturition and incontinence. Its mechanism of action involves the activation of β₃-adrenoreceptors. The present research outlines a novel, highly sensitive, and cost-effective protocol for analyzing MBG. The current research relies on MBG interaction with <i>o</i>-phthalaldehyde alongside 2-mercaptoethanol. The resulting isoindole fluorophore was quantified at <i>λ</i>_ex 337.7 and <i>λ</i>_em 433.7 nm. A comprehensive analysis was conducted to systematically examine and enhance the several experimental factors that impact the creation of the isoindole-based fluorophore outcome. A linear correlation was established by precisely manipulating these variables, demonstrating that the increase in fluorescence strength is directly correlated to the quantity of MBG within the range of 30 to 1000 ng/mL. The analytical method was able to reliably detect MBG concentrations as low as 9 ng/mL and accurately quantify levels down to 27.5 ng/mL. This analytical procedure was effectively employed to determine the concentration of MBG in commercially available tablet formulations, as well as in plasma/urine samples. Furthermore, the strategy was extended to assess the content uniformity of MBG-containing products, providing results with satisfactory accuracy and precision. Importantly, this approach is cost-effective, environmentally friendly, and can be readily implemented for quality control and routine monitoring of MBG in pharmaceutical products. The suggested approach was evaluated utilizing the RGB12 and BAGI tools. The outcomes abundantly show the practicability and integrity of the planned strategy.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Histidine Sequence-Associated Tripeptide Sequences for Recognition of Copper Ions and Their Application to Live Cells","authors":"Xue-jiao Wang,&nbsp;Jun-lei Hao,&nbsp;Peng-cheng Lin,&nbsp;Xiao Li,&nbsp;Shen-ao Li,&nbsp;Jing-xuan Wang,&nbsp;Shui-juan Liu,&nbsp;Zhong-chang Wang,&nbsp;Ming-yong Gao,&nbsp;Jiang Wu","doi":"10.1002/bio.70095","DOIUrl":"https://doi.org/10.1002/bio.70095","url":null,"abstract":"<div>\u0000 \u0000 <p>Copper is central to many enzymes in living organisms, and imbalances in copper levels are linked to various diseases. Therefore, developing probes to detect copper ions is essential. Histidine, especially in the polypeptide sequences at the first three N-terminal positions (His1, His2, and His3), uniquely binds to copper ions. This study introduces three groups of tripeptide probes designed to monitor copper ion levels in living cells and organisms. The results show that tripeptides with histidine at the −2 position, specifically HDQL-2 (Asp-His-Gln-Dansyl), HMFM-2 (Met-His-Phe-Dansyl), and HDMB-2 (Asp-His-Met-Dansyl), exhibit a higher affinity for copper ions. These probes responded quickly to copper ions, demonstrating excellent fluorescence turn-off performance and stable detection within a pH range of 6.0–11.0. The detection limits for fluorescence titration, calculated using the 3σ/k equation, were 17.65 nM (HDQL-2), 18.04 nM (HMFM-2), and 15.50 nM (HDMB-2). Peptide probes are ideal for detecting copper ions in living cells via fluorescence imaging because of their low toxicity and good biocompatibility. The fluorescence intensity decreases as copper ion content changes.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-Inspired Synthesis of Luminescent AgNPs Using Groundnut Oil Cake Extract for the Photocatalytic Degradation of Orange II Dye","authors":"Velu Manikandan,&nbsp;Velu Subash,&nbsp;Palaniyappan Jayanthi,&nbsp;Bontu Habtamu Geremew,&nbsp;R. Mythili,&nbsp;Kwang Soup Song","doi":"10.1002/bio.70118","DOIUrl":"https://doi.org/10.1002/bio.70118","url":null,"abstract":"<div>\u0000 \u0000 <p>The development of bio-fabrication techniques for nanoparticles has gained significant attention due to their cost-effectiveness, sustainability, and prospective for environmental applications. In this research, silver nanoparticles (AgNPs) were successfully fabricated using groundnut oil cake (GOC) extract as a reducing agent. The bioactive compounds in GOC, such as phenolic acids and flavonoids, played a crucial role in reducing silver nitrate into stable AgNPs in an aqueous solution. The use of GOC extract as a green reducing agent highlights the eco-friendly nature of the synthesis process. UV–visible spectroscopy confirmed the successful fabrication of AgNPs. The synthesis process was most efficient at a neutral pH of 7. The structural and morphological characteristics of the AgNPs were examined using advanced characterization methods such as EDS, TEM, FTIR, and XRD. TEM analysis revealed that the AgNPs ranged in size from 14 to 50 nm on average. The green-synthesized AgNPs showed outstanding photocatalytic performance, degrading 99.3% of Orange II dye in 120 min under visible light. Their spherical shape enhances charge separation and photocatalytic efficiency. The eco-friendly and cost-effective synthesis approach using GOC extract emphasizes the potential of these AgNPs for sustainable water purification and environmental remediation.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143380076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The FRET-Based APTA Sensor/Cy3 Complex for Glucose Determination","authors":"Ghasem Rezanejade Bardajee,&nbsp;Afsaneh Rahimi Chahrogh,&nbsp;Mojdeh Atashkadi","doi":"10.1002/bio.70097","DOIUrl":"https://doi.org/10.1002/bio.70097","url":null,"abstract":"<div>\u0000 \u0000 <p>This study developed a sensitive and cost-effective fluorescent probe based on the Förster Resonance Energy Transfer (FRET) method to monitor blood glucose levels. The APTA sensor/Cy3 probe consisted of cadmium telluride quantum dots modified with thioglycolic acid (CdTe-TGA QDs), a thiol-glucose-aptamer, and a Cy3-labeled aptamer. Due to the well-matched emission spectrum of the CdTe QDs and the absorption spectrum of Cy3, the FRET system decreased fluorescence intensity. However, glucose molecules quenched it when introduced to the system. The linear relationship between fluorescence intensity and glucose concentration was established with a detection limit of 7.72 × 10⁻⁹ M. The APTA sensor/Cy3 complex demonstrated excellent selectivity and specificity toward glucose and a high recovery rate of 96.00–101.11% in human serum and urine using the spiking method. The structural and morphological characteristics of the APTA sensor/Cy3 complex were confirmed by UV–visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS) analyses. The results suggest that the FRET-based APTA sensor/Cy3 complex would lead to the development of fluorescent probes for screening biological metabolites in clinical diagnostics and research.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide Fluorescent Probes Based on Aggregation-Induced Emission for the Detection of Ni2+ and Zn2+ in Different Buffer Systems","authors":"Dajiang Wang,&nbsp;Ping Ji,&nbsp;Xiaomei Liu,&nbsp;Xin Hu","doi":"10.1002/bio.70116","DOIUrl":"https://doi.org/10.1002/bio.70116","url":null,"abstract":"<div>\u0000 \u0000 <p>Heavy metal contamination has emerged as a significant global environmental concern. The contamination of Ni²⁺ and Zn²⁺ has attracted increasing attention, not only because of the pollution it causes but also because of the potential risks it poses to human health. It is of great importance to explore sensitive and rapid analytical methods for the accurate detection of Ni²⁺ and Zn²⁺. This paper presents the design and synthesis of a peptide fluorescent probe, TPE-HN (TPE-Pro-Trp-His-Glu-Phe-Gln-NH₂), coupled with a peptide to tetraphenylethylene (TPE). The aggregation-induced emission (AIE) effect has been employed to construct a rapid ‘turn-on’ assay for Ni²⁺and Zn²⁺ peptide fluorescent probes. The probe is capable of qualitatively detecting Ni²⁺ and Zn²⁺ in different buffer systems and can be distinguished by changes in buffer systems. The limit of detection for Ni²⁺ and Zn²⁺ in a 15% buffer solution was 9.613 mM (<i>R</i>² = 0.9924), whereas the limit of detection for Ni²⁺ in a 20% buffer solution was 1.215 mM (<i>R</i>² = 0.9922). The probe exhibits high sensitivity, high cell permeability and low biotoxicity, rendering it suitable for live-cell imaging under biological conditions. This demonstrates that TPE-HN is capable of detecting Ni²⁺ and Zn²⁺ in biological environments.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 2","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143362294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}