LuminescencePub Date : 2025-06-24DOI: 10.1002/bio.70222
Amal B. Ahmed, Mohammed E. Draz, Haydy Asad, Ibrahim A. Naguib, Fadwa H. Edrees
{"title":"Innovative Synchronous Spectrofluorometric Method for Assessing a Novel Drug Combination in Amyotrophic Lateral Sclerosis: In Vivo Human Application With Greenness and Sustainability Evaluation","authors":"Amal B. Ahmed, Mohammed E. Draz, Haydy Asad, Ibrahim A. Naguib, Fadwa H. Edrees","doi":"10.1002/bio.70222","DOIUrl":"10.1002/bio.70222","url":null,"abstract":"<div>\u0000 \u0000 <p>Amyotrophic lateral sclerosis (ALS) is a severe neurological disorder that causes damage to sensory neurons, then paralysis and death. A novel combination of celecoxib (CXP) and ciprofloxacin (CIP) has recently been used to enhance both motor performance and CNS cell morphology, alterations in the rate of disease progression, quality of life, and survival, which passed phase IIb RCT study. Celecoxib is classified as a non-steroidal anti-inflammatory drug; ciprofloxacin is a fluoroquinolone antibiotic that has a synergistic effect for the treatment of ALS, which is a severe neurological disorder. A new sustainable, simple, sensitive, and environmentally friendly synchronous spectrofluorimetric approach (SSF) was established to simultaneously estimate celecoxib and ciprofloxacin in pure form and biological fluids. The approach depends on synchronous fluorescence spectroscopy, where CXP and CIP were detected at 364 and 438 nm, correspondingly, using Δ<i>λ</i> of 80-nm utilizing sodium dodecyl sulphate (SDS) micellar system, which considerably improved synchronous fluorescence intensity. The approach was validated and revealed excellent linearity with concentrations varying from 10 to 10,000 and 5 to 20,000 ng/mL for CXP and CIP; correspondingly, CXP and CIP showed extremely low limits of detection (LODs) 0.58–0.24 ng/mL, which guarantee the sensitivity of the proposed approach. The suggested approach was successfully implemented to analyze the co-administered pharmaceuticals in their pure form and actual human plasma after concurrent oral administration of both drugs, which may be employed in an inquiry on the pharmacokinetics and bioavailability of human plasma to the new coming PrimeC pharmaceutical formulation. Ultimately, the method's remarkable greenness was proved by evaluating its greenness profile using various assessment strategies. The findings revealed that the SSF approach is a sustainable and environmentally friendly analytical approach.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Elucidating the Binding Mechanism of Oclacitinib With Bovine Serum Albumin: A Multidisciplinary Approach Combining Spectroscopy, Thermodynamics, and Molecular Docking","authors":"Dong Chen, Hui Gu, Jia-Ping Huang, Yu-Ting Wu, Rong-Juan Zhang, Shao-Liang Jiang, Jie-Hua Shi","doi":"10.1002/bio.70244","DOIUrl":"10.1002/bio.70244","url":null,"abstract":"<div>\u0000 \u0000 <p>This paper elucidates the in vitro binding of oclacitinib (OLTN) with BSA through a multifaceted approach that combines experimental methodologies and theoretical computations. Spectroscopic analyses reveal that OLTN significantly attenuates the intrinsical fluorescence of BSA through the formation of an OLTN–BSA complex, indicating a static quenching mechanism. Experimental data further confirmed the formation of a nonfluorescent OLTN–BSA complex. These findings provide valuable insights into the molecular interactions governing the OLTN–BSA system, enhancing our understanding of drug–protein binding dynamics. The binding constant and stoichiometric ratio of the complex were determined to be approximately 10<sup>4</sup> M<sup>−1</sup> and 1:1, respectively, suggesting moderate binding affinity of OLTN for BSA. Molecular docking assays verified that OLTN primarily associated with the Sudlow's Site II, inducing conformational alterations in both the secondary structure and quaternary structure of BSA. Thermodynamic and molecular docking analyses established that hydrophobicity interactions and hydrogen bonding (including conventional hydrogen bonding and the carbon hydrogen bonding) serve as the dominant driving forces for stabilizing the complex. This investigation provides critical guidance for understanding the pharmacodynamics and pharmacokinetics of OLTN in human, particularly regarding its plasma protein binding behavior and potential drug–drug interactions.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144472811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-19DOI: 10.1002/bio.70240
Panpan Yang, Chaoyue Wang, Ke Ying, Fengping Wang, Xiuping Wu, Xiaohong Zhu, Xionggao Han, Lisi Mao
{"title":"A Fluorescent Probe Derived From Methoxy-Modified Imidazo-Pyridin for Monitoring Sulfite in Chinese Medicinal Materials","authors":"Panpan Yang, Chaoyue Wang, Ke Ying, Fengping Wang, Xiuping Wu, Xiaohong Zhu, Xionggao Han, Lisi Mao","doi":"10.1002/bio.70240","DOIUrl":"10.1002/bio.70240","url":null,"abstract":"<div>\u0000 \u0000 <p>In this work, a methoxy-modified imidazo-pyridin–derived fluorescent probe <b>IPOMe-SF</b> was developed for monitoring sulfite in Chinese medicinal materials, and the imaging in living colorectal cells was conducted. Under the excitation of 355 nm, the response system with <b>IPOMe-SF</b> exhibited the concentration-dependent fluorescence enhancement at 490 nm along with the increase of the sulfite level. The response system suggested advantages including a relatively high sensitivity, high stability, rapid response, high selectivity, and low cytotoxicity. The particular privileges associated with the Chinese medicinal materials included the long linear response range and reliable anti-interference. <b>IPOMe-SF</b> inferred reliable steadiness for monitoring the sulfite level in the water decoction samples covered the “Zhebawei” herbal series. Further, the imaging system with <b>IPOMe-SF</b> realized the monitoring of both the exogenous and endogenous sulfite level, as well as the water decoction-treated status in colorectal cells. This work was meaningful for studying Chinese medicinal materials.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-18DOI: 10.1002/bio.70239
P. Varun Prasath, S. M. Muba Shera, K. Suganandam, G. Kalaiarasi, S. Sudhakar, K. Ravichandran, A. Kosiha
{"title":"Investigation on the Photodegradation Performance of S-Rich g-C3N4/MnO2 Composites for Efficient Removal of Hazardous Dyes From Aqueous Phase","authors":"P. Varun Prasath, S. M. Muba Shera, K. Suganandam, G. Kalaiarasi, S. Sudhakar, K. Ravichandran, A. Kosiha","doi":"10.1002/bio.70239","DOIUrl":"10.1002/bio.70239","url":null,"abstract":"<div>\u0000 \u0000 <p>Sulfur (S)-rich g-C<sub>3</sub>N<sub>4</sub>/α-MnO<sub>2</sub> (α-MnO<sub>2</sub>/GCN-S) heterojunction was synthesized using a simple hydrothermal technique, which was effectively utilized for the photodegradation of methylene blue and congo red dyes under visible light illumination. The results of the characterization analysis suggest that the α-MnO<sub>2</sub>/GCN-S composite exhibits a high crystalline nature and the heterojunction successfully formed between g-C<sub>3</sub>N<sub>4</sub> and α-MnO<sub>2</sub> demonstrates an effective photocatalytic agent for selected hazardous dyes. According to the obtained findings, α-MnO<sub>2</sub>/GCN-S composite exhibited outstanding visible light absorption when compared with pure g-C<sub>3</sub>N<sub>4</sub> and α-MnO<sub>2</sub> under visible light irradiation. The enhanced photocatalytic performance of the α-MnO<sub>2</sub>/GCN-S can be attributed to the effective separation of electron–hole pairs facilitated by g-C<sub>3</sub>N<sub>4</sub>, along with the improved visible light absorption properties of the S-doped α-MnO<sub>2</sub>. The findings indicate that the α-MnO<sub>2</sub>/GCN-S composite demonstrates exceptional photocatalytic performance, achieving degradation efficiencies of 91% and 92% for congo red and methylene blue in 30 and 60 min, respectively. On the other hand, the pristine form of GCN-S and α-MnO<sub>2</sub> exhibited lower degradation rates of 51% and 55% for congo red, and 67%, 69% for methylene blue, respectively. These results highlight the capability of α-MnO<sub>2</sub>/GCN-S composites to efficiently eliminate harmful dyes from aqueous solutions.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anticancer Effects of Monacolin X Against Human Liver Cancer Cell Line: Exploring the Apoptosis Using AO/EB and DCFHDA Fluorescent Staining","authors":"Vennila Jayaraman, Madan Kumar Arumugam, Shana Balachandran, Lokeshkumar Boopathy, Sasikumar Arumugam, Jamunarani Srirangaramasamy, Sandhanasamy Devanesan, Arumugam Suresh, Shobana Sampath","doi":"10.1002/bio.70229","DOIUrl":"10.1002/bio.70229","url":null,"abstract":"<div>\u0000 \u0000 <p>Hepatocellular carcinoma (HCC) most prevalent form of liver cancer and poses a few available treatments and is a major worldwide health burden. Monacolin X, a natural compound derived from the marine sponge-associated symbiont <i>Monascus ruber</i>, has garnered attention for its potential anticancer and anti-angiogenesis properties. This current study aimed to investigate the anticancer and apoptosis-inducing effects of Monacolin X against the human liver cancer (HepG2) cell line in vitro. This present study utilized various assays to assess cytotoxicity by MTT assay, apoptosis induction, DCFH-DA staining to measure the intracellular ROS levels, and apoptosis-and inflammation-related gene expression changes induced by the Monacolin X. The MTT results discovered dose-dependent cytotoxicity in HepG2 cells with an IC<sub>50</sub> value of 72.4 μM. The apoptosis-inducing effect of Monacolin X was evident through propidium iodide (PI) and acridine orange/ethidium bromide (AO/EB) staining, accompanied by increased intracellular ROS levels and downregulated expression of pro-inflammatory cytokines (<i>IL-6, IL-1β, TNF-α</i>) and modulation of key apoptosis regulators (<i>Bax</i> and <i>Bcl-2</i>) as determined by qPCR analysis. In conclusion, these observations suggest a mechanism whereby Monacolin X has potent anticancer activity against the HepG2 cell line, and further investigation will be required to determine the molecular pathways responsible for the potential therapeutic effects for the clinical implications in liver cancer.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-16DOI: 10.1002/bio.70237
Mohamed Ehab, E. Salama, Lina Sayed El-Ahll, H. A. Saudi, H. A. Abdel Ghany, Huda A. Alazab
{"title":"Preparation and Thermoluminescence Characteristics of Ytterbium Borosilicate Glasses for Radiation Dosimetry Applications","authors":"Mohamed Ehab, E. Salama, Lina Sayed El-Ahll, H. A. Saudi, H. A. Abdel Ghany, Huda A. Alazab","doi":"10.1002/bio.70237","DOIUrl":"10.1002/bio.70237","url":null,"abstract":"<div>\u0000 \u0000 <p>A borosilicate glass composite (SiO<sub>2</sub>—Na<sub>2</sub>CO<sub>3</sub>—ZnO—H<sub>3</sub>BO<sub>3</sub>) doped with varying concentrations of Yb<sub>2</sub>O<sub>3</sub> (0–0.1 mol%) was fabricated using the melt-quenching technique. The thermoluminescence (TL) properties of the prepared composite were investigated, revealing that the highest TL response (the total area under the curve) was achieved with 0.06 mol% Yb<sub>2</sub>O<sub>3</sub>. Subsequently, a comprehensive dosimetric analysis, considering annealing conditions, dose–response relationship, minimum detectable dose, reproducibility of the TL response, and thermal and optical fading of the prepared glass, was determined. The obtained results demonstrated that this glass composite exhibited a linear dose–response across a wide gamma dose range of 0.5–2 kGy and about 28% TL fading after 24 h of irradiation, followed by 16% after 7 days without any further deterioration during around 1 month of examination. These advantageous properties suggest that radiation detection applications can use this examined glass composite.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144299787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-13DOI: 10.1002/bio.70235
Minghan Bai, Yupeng Yang, Yuan Wen
{"title":"A Polysaccharide-Based Fluorescent Polymer Carrier for Natural Product Delivery and Gastric Cancer Inhibition","authors":"Minghan Bai, Yupeng Yang, Yuan Wen","doi":"10.1002/bio.70235","DOIUrl":"10.1002/bio.70235","url":null,"abstract":"<div>\u0000 \u0000 <p>In this study, we designed and synthesized a novel polysaccharide-based fluorescent nanocarrier system, HPMC-AuNPs@1, for the delivery of plant extract compound 1. The HPMC-AuNPs@1 system was synthesized by functionalizing gold nanoparticles (AuNPs) with hydroxypropyl methylcellulose (HPMC) and loading Compound 1 into the system. The resulting nanocarrier exhibited a typical aggregation-induced emission (AIE) effect in aqueous media, showing strong green fluorescence in a DMSO-H<sub>2</sub>O (5:95) solution. Host-guest sensing experiments revealed that HPMC-AuNPs@1 demonstrated high selectivity for detecting carcinoembryonic antigen (CEA) among 14 different metallic ions and antigen molecules. A noticeable fluorescence shift occurred upon binding with CEA, and the detection limit for CEA was as low as 2.56 × 10<sup>−6</sup> M. Additionally, we explored the potential therapeutic applications of HPMC-AuNPs@1 in cancer treatment, particularly in inducing pyroptosis, a form of programmed cell death. In vitro experiments with SNU-16 cells showed that HPMC-AuNPs@1 nanoparticles inhibited cancer cell proliferation by inducing pyroptosis. These findings suggest that HPMC-AuNPs@1 holds great promise as a therapeutic platform for cancer treatment, particularly in targeting cell proliferation pathways via pyroptosis induction.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-12DOI: 10.1002/bio.70236
Shen Zhang, Zhuo Wang, Yanan Li, Yuyu Guo
{"title":"Blue-Emissive Nitrogen-Doped Carbon Quantum Dots as Highly Effective Fluorescent Nanosensor for Congo Red Sensing","authors":"Shen Zhang, Zhuo Wang, Yanan Li, Yuyu Guo","doi":"10.1002/bio.70236","DOIUrl":"10.1002/bio.70236","url":null,"abstract":"<div>\u0000 \u0000 <p>Based on the necessity of Congo red detection and excellent performance of the fluorescence method, nitrogen-doped carbon quantum dots (N-CQDs) were established through a facile and green hydrothermal method. N-CQDs were synthesized from hawthorn leaves and N-isopropylacrylamide and were firstly used for Congo red detection. Through the inner filter effect and static quenching principle, this as-prepared probe was successfully applied in the sensitive and selective detection of Congo red. Combining various characterization results, well-dispersed N-CQDs possessed superior fluorescent property, water solubility, and stability. The blue fluorescence emitted at 438 nm when the maximum excitation wavelength was located at 365 nm. Under the optimal detecting condition, the linear range achieved 0.5–300 μM with a detection limit of 0.089 μM. More importantly, the outstanding performance of recovery experiments in real samples and temperature sensing displayed great promise for Congo red detection through N-CQDs.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-12DOI: 10.1002/bio.70223
Arumugam Kathirvel, Ramalingam Srinivasan, Muthusamy Sasikumar, Natarajan Ranjith, Ramasamy Srinivasan, Rajamanickam Karthika, Raji Atchudan, Devarajan Natarajan, Mohammad Ahmad Wadaan, P. Srinivasan, Hae Keun Yun
{"title":"Green Synthesis of ZrO2 Nanoparticles From Gardenia resinifera for Efficient Photocatalytic Methylene Blue Dye Degradation With Antimicrobial and Antioxidant Properties","authors":"Arumugam Kathirvel, Ramalingam Srinivasan, Muthusamy Sasikumar, Natarajan Ranjith, Ramasamy Srinivasan, Rajamanickam Karthika, Raji Atchudan, Devarajan Natarajan, Mohammad Ahmad Wadaan, P. Srinivasan, Hae Keun Yun","doi":"10.1002/bio.70223","DOIUrl":"10.1002/bio.70223","url":null,"abstract":"<div>\u0000 \u0000 <p>This study focuses on the green synthesis of zirconium oxide nanoparticles (ZrO<sub>2</sub> NPs) using <i>Gardenia resinifera</i> leaf extract and evaluates their multifunctional applications. The synthesized ZrO<sub>2</sub> nanoparticles (Gr-ZrO<sub>2</sub> NPs) were characterized through various optical and structural analyses. UV-Vis spectroscopy showed peak absorbance at 294 and 404 nm. FTIR analysis identified phytochemical functional groups from the leaf extract involved in nanoparticle formation. X-ray diffraction confirmed the monoclinic and tetragonal phases of the Gr-ZrO<sub>2</sub> NPs, supported by SAED patterns. SEM images revealed spherical morphology, while EDX spectra confirmed the presence of Zr (60.79%) and O (39.21%) elements. TEM analysis indicated spherical nanostructures with a diameter of approximately 10 nm. Gr-ZrO<sub>2</sub> NPs demonstrated a significant methylene blue dye removal efficiency of over 91% within 3 h under the sunlight. They also showed notable antibacterial activity, with MIC value ranging from 2.80 ± 0.27 to 6.03 ± 0.10 μg/mL. Gr-ZrO<sub>2</sub> NPs exhibited significant antioxidant activity; the lowest IC<sub>50</sub> value (14.88 ± 0.04 μg/mL) was recorded for nitric oxide radical scavenging. These results suggest that the synthesized Gr-ZrO<sub>2</sub> NPs are effective as antioxidants and antimicrobials and hold promise as photocatalytic agents, potentially serving as candidates for environmental remediation, water treatment, and biomedicine.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144273497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2025-06-11DOI: 10.1002/bio.70231
Sumathy Rengarajan, Renuga Devi Navaneethan, S. Alagendran, C. Subramani, Sukirtha Raman, Saurav Dixit, R. Mythili, Bharathiraja Chinnapandi, Arjun Pandian, R. K. Govindarajan
{"title":"Exploring the Nanoparticle Fabrication Ability of Amaranthus spinosus and Its Biological Functions","authors":"Sumathy Rengarajan, Renuga Devi Navaneethan, S. Alagendran, C. Subramani, Sukirtha Raman, Saurav Dixit, R. Mythili, Bharathiraja Chinnapandi, Arjun Pandian, R. K. Govindarajan","doi":"10.1002/bio.70231","DOIUrl":"10.1002/bio.70231","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Amaranthus spinosus</i> is a medicinal plant with notable pharmacological properties. In the present work, silver nanoparticles (AgNPs) were synthesized using methanolic leaf extract of <i>A. spinosus</i>. The synthesis of AgNPs was indicated by a color change from green to brown and confirmed through UV–Vis spectroscopy, FTIR, SEM, and XRD analyses. The XRD peaks between 20° and 50° confirmed the crystalline nature of AgNPs, while SEM (1 μm scale) revealed uniformly distributed spherical nanoparticles below 100 nm. The AgNPs exhibited potent antimicrobial activity, with the highest inhibition observed against <i>Klebsiella pneumoniae</i> (20 mm at 100 μg), surpassing the plant extract. Antioxidant assays proved the free radical scavenging capacity of AgNPs compared with standard ascorbic acid. At 500 μg/mL, AgNPs achieved 71% DPPH scavenging, 75% hydroxyl radical scavenging, and 68% reducing power, significantly outperforming the plant extract. The in vitro anticancer property of AgNPs against HT-29 colorectal cancer cells revealed a concentration-dependent cytotoxic effect, with maximum cell death at 100 μg/mL. AO/EtBr staining confirmed apoptosis through a shift from green to orange/red fluorescence, while DAPI staining indicated nuclear condensation and fragmentation. These findings highlight <i>A. spinosus</i>-derived AgNPs as promising candidates for biomedical applications, offering enhanced antimicrobial, antioxidant, and anticancer properties via eco-friendly synthesis.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 6","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}