LuminescencePub Date : 2024-11-07DOI: 10.1002/bio.70028
Du Liu, Hongyan An, Xianglong Li, Benhua Wang, Shaojing Zhao, Minhuan Lan, Zhanhong Yang, Xiangzhi Song
{"title":"Lysosome-Targeted Bifunctional Fluorescent Probe and Type I/II Photosensitizer for Viscosity Imaging and Cancer Photodynamic Therapy","authors":"Du Liu, Hongyan An, Xianglong Li, Benhua Wang, Shaojing Zhao, Minhuan Lan, Zhanhong Yang, Xiangzhi Song","doi":"10.1002/bio.70028","DOIUrl":"10.1002/bio.70028","url":null,"abstract":"<div>\u0000 \u0000 <p>Abnormal lysosomal viscosity is closely associated with cancer progression, underscoring the need for bifunctional fluorescent probes and photosensitizers (PSs) that can both monitor viscosity and facilitate imaging-guided therapy for simultaneous cancer diagnosis and treatment. Despite advances in lysosome-targeted PSs development, few have demonstrated the ability to generate both Type I and Type II reactive oxygen species (ROS). In this study, we present <b>BTTPA</b>, a lysosome-targeted fluorescent probe and photosensitizer, designed to integrate cancer diagnosis via viscosity imaging and cancer treatment through photodynamic therapy (PDT). Our findings reveal that <b>BTTPA</b> selectively targets lysosomes, enabling dynamic monitoring of cellular viscosity and distinguishing cancer cells from normal cells. Upon light activation, <b>BTTPA</b> efficiently generates both Type I and Type II ROS. Apoptosis assays further confirm <b>BTTPA</b>'s effectiveness in inducing cancer cell apoptosis, highlighting its potential as a powerful tool for cancer diagnosis and therapy.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-07DOI: 10.1002/bio.70027
Zhigang Wang, Jiayu Wang, Dangli Gao, Wenqian Xu, Wenna Gao, Xiangyu Zhang, Sining Yun, Xiaojun Wang
{"title":"Cr3+-Activated Deep Trap Electron-Trapping Biphasic Mixture of Zn (Al,Ga)2O4 and Zn2GeO4 for Multilevel Information Storage by Trap Multiplexing and Wavelength Multiplexing","authors":"Zhigang Wang, Jiayu Wang, Dangli Gao, Wenqian Xu, Wenna Gao, Xiangyu Zhang, Sining Yun, Xiaojun Wang","doi":"10.1002/bio.70027","DOIUrl":"10.1002/bio.70027","url":null,"abstract":"<div>\u0000 \u0000 <p>Double wavelength emitting phosphors with hypersensitive thermal response are important for optical/thermal sensors and multiplexing optical data storage technology. However, it is a daunting challenge to develop multicolor electron-trapping materials with superheat sensitivity and anomalous quenching luminescence characteristics for information storage. Here, based on the selection of host lattice and the control of doping ion types, we have designed Mn<sup>2+</sup>- and/or Cr<sup>3+</sup>-activated persistent materials with excellent fluorescence properties. Among these phosphors, Mn,Cr co-activated biphasic mixture exhibits not only sensitive anomalous thermosensitive fluorescence properties but also multicolor and multimode fluorescence emission characteristics derived from Mn and Cr simultaneously. The corresponding multimode fluorescence mechanism is proposed based on afterglow energy transfer and deepening trap effect. Especially, based on the excellent fluorescence performances, these phosphors as information storage medium demonstrate multilevel information storage by a combination of the trap multiplexing and wavelength multiplexing technologies. The study here provides not only the ideas for designing and synthesizing new electronic materials but also a material foundation for the fourth generation of optical information storage and encryption.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":"1-8"},"PeriodicalIF":3.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-07DOI: 10.1002/bio.70013
Qi Tan, Bingliang Gao, Pengde Gao, Chao Fan, Haijun Li
{"title":"Quantitative Analysis of Complex Aluminum Electrolytes by X-Ray Fluorescence","authors":"Qi Tan, Bingliang Gao, Pengde Gao, Chao Fan, Haijun Li","doi":"10.1002/bio.70013","DOIUrl":"10.1002/bio.70013","url":null,"abstract":"<div>\u0000 \u0000 <p>The future production of electrolytic aluminum will be characterized by low temperature, low carbon emissions, and environmental friendliness, which will result in a more complex electrolyte system. Real-time analysis of electrolytes can significantly enhance the efficiency of the electrolytic process. However, accurately analyzing the composition of complex aluminum electrolytes online has been a technical challenge. This research primarily focuses on determining the ingredient contents of complex aluminum electrolytes based on elemental concentrations measured using X-ray fluorescence (XRF) according to an applicable standard. Calibration curves were established based on 53 standard samples of complex aluminum electrolytes to establish relationships between XRF fluorescence intensity and real concentrations. Analysis conducted on industrial complex electrolytes confirms that these newly constructed curves effectively reduce relative errors to less than 3%. Additionally, a feasible solution is proposed for determining the cryolite ratio (CR) in aluminum electrolytes using XRF. Results demonstrate an average deviation as low as approximately 0.02, fully meeting industrial production requirements. This technique offers numerous benefits including rapid analysis, ease of use, and cost savings.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dual “Turn-On” Fluorescent and Colorimetric Sensing of Permanganate Based on Yellow Carbon Dots","authors":"Hui-Hui Zeng, Ren-Xiu Huang, Yue Qiu, Ju-Lian Duan, Shu-Cheng Jiang","doi":"10.1002/bio.70011","DOIUrl":"10.1002/bio.70011","url":null,"abstract":"<div>\u0000 \u0000 <p>Existing permanganates (MnO<sub>4</sub><sup>−</sup>) sensing methods suffered from the poor selectivity, restricted solubility, and/or less desirable signaling mechanisms (e.g., “turn-off” processes), resulting in the false positive result. In this work, an effective “turn-on” fluorescence method for MnO<sub>4</sub><sup>−</sup> detection in aqueous media was proposed via yellow fluorescence carbon dots (Y-CDs) with the maximum emission peak of 553 nm. Upon the introduction of MnO<sub>4</sub><sup>−</sup>, the fluorescence of Y-CDs increased significantly due to the enhanced surface passivation degree and intramolecular charge transfer. In the range of 0.1–10 μM, the fluorescence ratio (F/F<sub>0</sub>) is proportional to the MnO<sub>4</sub><sup>−</sup> ions concentration, realizing the “turn-on” fluorescence sensing of MnO<sub>4</sub><sup>−</sup>. On the other hand, utilizing the significant color change of Y-CDs by MnO<sub>4</sub><sup>−</sup> ions, the colorimetric analysis can also be established for MnO<sub>4</sub><sup>−</sup> assay. Compared with other probes, the analysis results obtained by bimodal sensing tactics of fluorescent and colorimetric can be mutually verified to improve the reliability and meet the sensing needs of targets in complex environments.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-06DOI: 10.1002/bio.70022
Aryan Fathulla Qader
{"title":"Validated Spectrofluorometric Assay for Folic Acid Determination in Pure and Pharmaceutical Forms via Basic Fuchsin Fluorescence Quenching","authors":"Aryan Fathulla Qader","doi":"10.1002/bio.70022","DOIUrl":"10.1002/bio.70022","url":null,"abstract":"<div>\u0000 \u0000 <p>A simple, specific, sensitive, rapid, and cost-effective spectrofluorometric method for quantifying folic acid has been developed using basic fuchsin as a fluorescence quenching agent. This method relies on the quenching effect of folic acid on the inherent fluorescence of basic fuchsin, facilitated by their interaction in Britton–Robinson buffer solution at pH 9, leading to the formation of an ion-associated complex. The decrease in fluorescence intensity of basic fuchsin was measured at 730 nm, with excitation at 365 nm. The method demonstrated a linear relationship (<i>R</i><sup>2</sup> = 0.9977) between the quenching fluorescence intensity (ΔF) and folic acid concentration over the range of 4–20 μg/mL, with a detection limit of 0.17 μg/mL. The method showed no significant interference from common excipients found in pharmaceutical tablets. The results obtained were in good agreement with those from high-performance liquid chromatography, with no significant differences in precision or accuracy. This spectrofluorimetric method was successfully applied to determine the folic acid content in various commercial pharmaceutical tablets.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-05DOI: 10.1002/bio.70016
Rajamani Vanitha, Abhijit Debnath, M. Mohana Keerthi, Julius Uchoi, Loganathan Subha, Mysoon M. Al-Ansari, P. Srinivasan, Chandramohan Kavitha
{"title":"Development of Luminescent and Photosensitive Cellulose Microfibers From Palm Sprout Fibers: Antibacterial Efficacy and Dye Adsorption Potential","authors":"Rajamani Vanitha, Abhijit Debnath, M. Mohana Keerthi, Julius Uchoi, Loganathan Subha, Mysoon M. Al-Ansari, P. Srinivasan, Chandramohan Kavitha","doi":"10.1002/bio.70016","DOIUrl":"10.1002/bio.70016","url":null,"abstract":"<div>\u0000 \u0000 <p>A key contemporary challenge is enhancing the value of agro-industrial byproducts. Cellulose, the most abundant renewable resource, offers significant industrial potential due to its versatile properties. Produced in its pure form by various bacteria, cellulose is increasingly utilized in microscale and nanoscale fibers for composite reinforcement. In this study, cellulose microfibers were derived from palm sprout fibers through a series of treatments like pretreatment, high-pressure hydrolysis in an autoclave, and bleaching. These fibers were then characterized using techniques such as UV spectroscopy, FTIR, SEM, and XRD. The study evaluated the antibacterial properties of the treated cellulose microfibers against both gram-positive <i>Staphylococcus aureus</i> (07%) and gram-negative <i>Escherichia coli</i> (10%), finding higher effectiveness against <i>E.coli</i>. Additionally, the microfibers exhibited a lower EC<sub>50</sub> value of 0.101 mg/mL, suggesting that although the microfiber extract is effective, the standard antioxidant is somewhat more potent. Adsorption studies revealed that the cellulose microfibers followed the Langmuir isotherm model, with a high determination coefficient of 0.998, and reached maximum dye adsorption for RB 160 within 120 min. In this adsorption study, 50 mg of dye was removed. These results indicate that the treated cellulose microfibers are a promising biosorbent for improving dye removal processes.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-05DOI: 10.1002/bio.70018
N. Vijaya, S. Daisylin Anbu Sujitha, Amalraj Shankar, Suhas Khot, C. Barath Kanna, Mohamad S. AlSalhi, T. Selvankumar, Seong-Cheol Kim, Madhappan Santhamoorthy, Pugalenthi Ramesh
{"title":"ZIF-67 derivatives of NiCo2S4 Decorated in Salen-Complex Amine Functionalization GO Layers for High-Performance Applications in Supercapacitor Devices","authors":"N. Vijaya, S. Daisylin Anbu Sujitha, Amalraj Shankar, Suhas Khot, C. Barath Kanna, Mohamad S. AlSalhi, T. Selvankumar, Seong-Cheol Kim, Madhappan Santhamoorthy, Pugalenthi Ramesh","doi":"10.1002/bio.70018","DOIUrl":"10.1002/bio.70018","url":null,"abstract":"<div>\u0000 \u0000 <p>The study develops polyamine-functionalized graphene oxide–supported NiCo<sub>2</sub>S<sub>4</sub> nanomaterial using a metal–organic framework (MOF). This modification adds free amines and oxygen functionality to the graphene oxide electrode surface, resulting in the decrease in the 2 theta value from 11.2 to 7.1 and an increase in the interlayer spacing value from 1.4 to 7.8 nm. This modification enhances the surface properties, leading to improved wettability and dispersion stability. Additionally, bimetallic nanoparticles of NiCo<sub>2</sub>S<sub>4</sub>, synthesized via the ZIF-67 MOF template, further enhance performance. Analysis confirms the successful fabrication of ZIF-67/f-GO/NiCo<sub>2</sub>S<sub>4</sub> electrode material through various techniques such as XPS spectra, FE-SEM, XRD, and Raman spectra. The resulting Salen-fGO@NiCo<sub>2</sub>S<sub>4</sub> composite exhibits impressive specific capacitance, reaching 2581.65 F g<sup>−1</sup> at 10 A g<sup>−1</sup> with 82% capacity retention after 5000 cycles. Employing this composite in a hybrid supercapacitor yields remarkable energy and power densities of 40 and 920 W kg<sup>−1</sup>, individually, while maintaining 74% capacity retention rate at 10 A g<sup>−1</sup>. These findings offer promising prospects for advanced energy storage applications.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-05DOI: 10.1002/bio.70010
Chandran Aswathy R, Jacob Linju Ann, Paul Issac, Jose Gijo, Mathew Prijil, Sisira S
{"title":"Spectral Investigation of Enhanced Orange Emission in Eu3+ Activated LaPO4 Nanophosphors Through Codoping With Na+ and Bi3+ Ions","authors":"Chandran Aswathy R, Jacob Linju Ann, Paul Issac, Jose Gijo, Mathew Prijil, Sisira S","doi":"10.1002/bio.70010","DOIUrl":"10.1002/bio.70010","url":null,"abstract":"<p>Lanthanum phosphate is a well-known potential host for rare earth activators for phosphor-converted white LED application. Na<sup>+</sup> and Bi<sup>3+</sup> codoped Eu<sup>3+</sup> activated LaPO<sub>4</sub> nanophosphor is successfully synthesized using citrate-based hydrothermal method at ambient condition and confirmed by XRD, EDS and XPS analysis. The excitation spectra of the sample exhibit sharp f-f characteristics peak of Eu<sup>3+</sup> at 393 nm that is found to be the most dominant. The comparative photoluminescence studies of La<sub>1-x</sub>PO<sub>4</sub>:xEu<sup>3+</sup>(<i>x</i> = 0.10), La<sub>1-x-y</sub> PO<sub>4</sub>:xEu<sup>3+</sup>, yNa<sup>+</sup>(<i>x</i> = 0.10, <i>y</i> = 0.05), and La<sub>1-x-y-z</sub>PO<sub>4</sub>:xEu<sup>3+</sup>, yNa<sup>+</sup>, zBi<sup>3+</sup> (<i>x</i> = 0.10, <i>y</i> = <i>z</i> = 0.05) reveal a progressive enhancement of the orange emission from Eu<sup>3+</sup> as Na<sup>+</sup> and (Na<sup>+</sup>, Bi<sup>3+</sup>) ions are incorporated into the LaPO<sub>4</sub> matrix. The dominance of <sup>5</sup>D<sub>0</sub>-<sup>7</sup>F<sub>1</sub> magnetic dipole transition contributing a major part of orange luminescence of the nanophosphors and the symmetric site occupancy of Eu<sup>3+</sup> was justified from the observed trend of <span></span><math>\u0000 <semantics>\u0000 <mrow>\u0000 <msub>\u0000 <mi>Ω</mi>\u0000 <mn>4</mn>\u0000 </msub>\u0000 <mo>></mo>\u0000 <msub>\u0000 <mi>Ω</mi>\u0000 <mn>2</mn>\u0000 </msub>\u0000 </mrow>\u0000 <annotation>$$ {varOmega}_4>{varOmega}_2 $$</annotation>\u0000 </semantics></math> in the Judd–Ofelt intensity parameters. The CIE studies confirm the improvement in color purity of emission from 32.5% to 90.6% due to the presence of Na<sup>+</sup> and Bi<sup>3+</sup>ions.</p>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142584794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-11-04DOI: 10.1002/bio.70023
Minghao He, Qian Chen, Yuxin Wang, Qianqian Zhu, Hong Xu
{"title":"Spectroscopic Studies on Maillard Reactions of Peanut Ara h 2.01 With Respective Reducing Sugars and Their Impacts on Ara h 2.01 Allergenicity","authors":"Minghao He, Qian Chen, Yuxin Wang, Qianqian Zhu, Hong Xu","doi":"10.1002/bio.70023","DOIUrl":"10.1002/bio.70023","url":null,"abstract":"<div>\u0000 \u0000 <p>The effects of the Maillard reactions of peanut Ara h 2.01 with respective glucose and xylose on its conformation and allergenicity were investigated. Circular dichroism (CD) spectral studies showed that short-term heating alone with reducing sugars did not change protein secondary structures, but after undergoing Maillard reactions, its secondary structures changed with some α-helices being transformed into β-sheets and random coils. Fluorescence spectral studies indicated that as temperature increased, protein became loose and extended induced by the reducing sugars. Long heat treatment at higher temperature caused protein collapse with the extrusion of its internal water molecules, which was hindered partially by the reducing sugars that entered the protein in Maillard reaction. Resonance light scattering (RLS) spectra confirmed that more xylose molecules entered protein due to its stronger interaction with the protein after Maillard reactions. ELISA assays exhibited that heat treatment at 100°C led to some allergenic epitopes being embedded inside protein molecules due to protein collapse and that partial epitopes were covered by the reducing sugars after Maillard reaction, resulting in decreases in its allergenicity. Also, xylose reduced the allergenicity of protein more efficiently owing to the stronger interaction between them. All above experimental results were explained reasonably by bioinformatic analysis and molecular docking.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142577145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
LuminescencePub Date : 2024-10-31DOI: 10.1002/bio.70000
Ejlal Mohamed Abdullah, Farid Shokry Ataya, Md. Tabish Rehman, Mohammed Arshad, Abdul Aziz Al Kheraif, Nojood Al-twaijry, Atekah Hazza Alshammari, Mohammad F. AlAjmi, Majed S. Alokail, Mohd Shahnawaz Khan
{"title":"Binding of a Drug (Colchicine) to L-Asparaginase Enzyme Using Multispectroscopic, Thermodynamics, and Simulation Studies: Possible Implication in Acute Lymphoblastic Leukemia Treatment","authors":"Ejlal Mohamed Abdullah, Farid Shokry Ataya, Md. Tabish Rehman, Mohammed Arshad, Abdul Aziz Al Kheraif, Nojood Al-twaijry, Atekah Hazza Alshammari, Mohammad F. AlAjmi, Majed S. Alokail, Mohd Shahnawaz Khan","doi":"10.1002/bio.70000","DOIUrl":"10.1002/bio.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>The research aims to elucidate how drug interactions affect the activity of L-asparaginase (L-ASNase), an essential enzyme in cancer treatment, especially for acute lymphoblastic leukemia (ALL). Understanding these interactions is crucial for optimizing treatment effectiveness and reducing adverse effects. This study explores the intricate molecular interactions and structural dynamics of L-ASNase upon binding with colchicine. Fluorescence quenching experiments were conducted at various temperatures (298, 303, and 310 K), revealing notable interactions between L-ASNase and colchicine. These interactions were characterized by a reduction in fluorescence intensity and a blue shift in emission maxima. Additional analyses, including the determination of Stern–Volmer quenching constants (<i>K</i><sub>SV</sub>), bimolecular quenching rate constants (<i>k</i><sub>q</sub>), and thermodynamic parameters, indicated a static quenching mechanism with moderate binding affinities (<i>K</i><sub>a</sub>: 1.40–2.71 × 10<sup>4</sup> M<sup>−1</sup>) across different temperatures. Thermodynamic study suggested positive enthalpy and entropy changes (Δ<i>H</i>° = −10.26 kcal mol<sup>−1</sup>; Δ<i>S</i>° = −14.19 cal mol<sup>−1</sup> K<sup>−1</sup>), suggesting a spontaneous reaction with negative Δ<i>G</i>° values (−5.86 to −6.03 kcal mol<sup>−1</sup>). FRET measurements supported optimal distances (<i>r</i> and <i>R</i><sub>o</sub>) for FRET occurrence, reinforcing the static quenching mechanism. Molecular docking further supported these findings, revealing a 1:1 stoichiometric binding ratio for L-ASNase:colchicine and elucidating specific binding orientations and interactions critical for complex stability. Subsequent molecular dynamics simulations spanning 100 ns underscored the stability of the L-ASNase–colchicine complex, with minimal deviations observed in key structural parameters such as RMSD, RMSF, R<sub>g</sub>, and SASA. Additionally, spectroscopic analyses, including circular dichroism (CD), synchronous fluorescence, and 3D fluorescence provided insights into the conformational changes and alterations in the microenvironment of aromatic amino acid residues in L-ASNase upon colchicine binding. Moreover, L-ASNase activity was slightly reduced by 25% in the presence of colchicine. This comprehensive investigation sheds light on the molecular intricacies of the L-ASNase–colchicine complex, advancing our understanding of drug–target interactions and offering potential avenues for therapeutic applications.</p>\u0000 </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"39 11","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142548609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}