从苹果蜗牛卵中提取可持续荧光碳点,增强潜在指纹中脱皮细胞的可视化

IF 3 4区 化学 Q2 CHEMISTRY, ANALYTICAL
Luminescence Pub Date : 2025-06-25 DOI:10.1002/bio.70246
Ratima Janthima, Oratai Weeranantanapan, Sirapanat Injinda, Sineenat Siri
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引用次数: 0

摘要

潜在指纹包含脱落的皮肤细胞,作为法医分析的关键DNA来源;然而,触摸DNA分析的效率往往受到细胞数量不足的限制。本研究提出了一种新型荧光碳点(CD),用于增强细胞在潜在指纹图谱中的可见性。采用微波辅助水热法合成ASE衍生CDs (ASE-CDs),优化条件为0.1 g/mL ASE提取物,温度为1200 W,温度为200℃,反应时间为60 min。所得的ASE-CDs具有较强的荧光,激发峰和发射峰分别在351 nm和416 nm处。透射电子显微镜(TEM)显示其形貌均匀,平均粒径为5.4 nm, x射线衍射(XRD)和傅里叶变换红外光谱(FT-IR)证实了其结晶度、石墨化程度和表面官能团。ASE-CDs能够在潜在指纹中有效地显示脱落的皮肤细胞,显示出比常用染料(钻石核酸染料(DD)和SYBR Green I核酸凝胶染色(SGI))更高的荧光强度,同时显示出比DD更低的光漂白。这些发现突出了ASE-CDs是一种可持续的、环保的荧光探针,具有潜在的法医应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Sustainable Fluorescent Carbon Dots From Apple Snail Eggs for Enhanced Visualization of Shed Skin Cells in Latent Fingerprints

Latent fingerprints contain shed skin cells that serve as critical DNA sources for forensic analysis; however, the efficiency of touch DNA analysis is often limited by insufficient cell quantities. This study presents a novel fluorescent carbon dot (CD) synthesized from a sustainable biomass source—apple snail eggs (ASE)—to enhance cell visualization in latent fingerprints. The ASE-derived CDs (ASE-CDs) were synthesized via a microwave-assisted hydrothermal method, optimized with 0.1-g/mL ASE extract at 1200 W, 200°C, for 60 min. The resulting ASE-CDs exhibited strong fluorescence, with excitation and emission peaks at 351 and 416 nm, respectively. Transmission electron microscopy (TEM) revealed a uniform morphology with an average particle size of 5.4 nm, while X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy confirmed their crystallinity, graphitization, and surface functional groups. ASE-CDs enabled the effective visualization of shed skin cells in latent fingerprints, displaying higher fluorescence intensity than commonly used dyes—Diamond Nucleic Acid Dye (DD) and SYBR Green I Nucleic Acid Gel Stain (SGI)—while demonstrating lower photobleaching than DD. These findings highlight ASE-CDs as a sustainable, eco-friendly fluorescent probe with potential forensic applications.

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来源期刊
Luminescence
Luminescence 生物-生化与分子生物学
CiteScore
5.10
自引率
13.80%
发文量
248
审稿时长
3.5 months
期刊介绍: Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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