Microbiology-Sgm最新文献_第8页

Time-lapse mesoscopy of Candida albicans and Staphylococcus aureus dual-species biofilms reveals a structural role for the hyphae of C. albicans in biofilm formation. 对白色念珠菌和金黄色葡萄球菌双种生物膜的延时介观观察揭示了白色念珠菌菌丝在生物膜形成过程中的结构作用。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001426

Katherine J Baxter, Fiona A Sargison, J Ross Fitzgerald, Gail McConnell, Paul A Hoskisson

{"title":"Time-lapse mesoscopy of Candida albicans and Staphylococcus aureus dual-species biofilms reveals a structural role for the hyphae of C. albicans in biofilm formation.","authors":"Katherine J Baxter, Fiona A Sargison, J Ross Fitzgerald, Gail McConnell, Paul A Hoskisson","doi":"10.1099/mic.0.001426","DOIUrl":"10.1099/mic.0.001426","url":null,"abstract":"Polymicrobial infection with Candida albicans and Staphylococcus aureus may result in a concomitant increase in virulence and resistance to antimicrobial drugs. This enhanced pathogenicity phenotype is mediated by numerous factors, including metabolic processes and direct interaction of S. aureus with C. albicans hyphae. The overall structure of biofilms is known to contribute to their recalcitrance to treatment, although the dynamics of direct interaction between species and how it contributes to pathogenicity is poorly understood. To address this, a novel time-lapse mesoscopic optical imaging method was developed to enable the formation of C. albicans/S. aureus whole dual-species biofilms to be followed. It was found that yeast-form or hyphal-form C. albicans in the biofilm founder population profoundly affects the structure of the biofilm as it matures. Different sub-populations of C. albicans and S. aureus arise within each biofilm as a result of the different C. albicans morphotypes, resulting in distinct sub-regions. These data reveal that C. albicans cell morphology is pivotal in the development of global biofilm architecture and the emergence of colony macrostructures and may temporally influence synergy in infection.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139543325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a system for hydroxamate xenosiderophore-mediated iron transport in Burkholderia cenocepacia. 确定伯克霍尔德氏菌（Burkholderia cenocepacia）中由羟基氨基甲酸酯异苷酸盐介导的铁运输系统。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001425

Syakira Mohammed Hussein, Aderonke Sofoluwe, Ameya Paleja, Anne Duhme-Klair, Mark S Thomas

{"title":"Identification of a system for hydroxamate xenosiderophore-mediated iron transport in Burkholderia cenocepacia.","authors":"Syakira Mohammed Hussein, Aderonke Sofoluwe, Ameya Paleja, Anne Duhme-Klair, Mark S Thomas","doi":"10.1099/mic.0.001425","DOIUrl":"10.1099/mic.0.001425","url":null,"abstract":"One of the mechanisms employed by the opportunistic pathogen Burkholderia cenocepacia to acquire the essential element iron is the production and release of two ferric iron chelating compounds (siderophores), ornibactin and pyochelin. Here we show that B. cenocepacia is also able to take advantage of a range of siderophores produced by other bacteria and fungi ('xenosiderophores') that chelate iron exclusively by means of hydroxamate groups. These include the tris-hydroxamate siderophores ferrioxamine B, ferrichrome, ferricrocin and triacetylfusarinine C, the bis-hydroxamates alcaligin and rhodotorulic acid, and the monohydroxamate siderophore cepabactin. We also show that of the 24 TonB-dependent transporters encoded by the B. cenocepacia genome, two (FhuA and FeuA) are involved in the uptake of hydroxamate xenosiderophores, with FhuA serving as the exclusive transporter of iron-loaded ferrioxamine B, triacetylfusarinine C, alcaligin and rhodotorulic acid, while both FhuA and FeuA are able to translocate ferrichrome-type siderophores across the outer membrane. Finally, we identified FhuB, a putative cytoplasmic membrane-anchored ferric-siderophore reductase, as being obligatory for utilization of all of the tested bis- and tris-hydroxamate xenosiderophores apart from alcaligin.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139378666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Primer: The bacterial flagellum - how bacteria swim. 微生物入门：细菌鞭毛--细菌如何游泳。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001406

Judith P Armitage

引用次数: 0

Construction and characterisation of a structured, tuneable, and transparent 3D culture platform for soil bacteria. 构建结构化、可调节和透明的土壤细菌三维培养平台并确定其特性。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001429

Liam M Rooney, Lionel X Dupuy, Paul A Hoskisson, Gail McConnell

引用次数: 0

An assessment of the airborne longevity of group A Streptococcus. 评估 A 群链球菌在空气中的寿命。

IF 2.6 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001421

Henry P Oswin, Evie Blake, Allen E Haddrell, Adam Finn, Shiranee Sriskandan, Jonathan P Reid, Alice Halliday, Anu Goenka

{"title":"An assessment of the airborne longevity of group A Streptococcus.","authors":"Henry P Oswin, Evie Blake, Allen E Haddrell, Adam Finn, Shiranee Sriskandan, Jonathan P Reid, Alice Halliday, Anu Goenka","doi":"10.1099/mic.0.001421","DOIUrl":"10.1099/mic.0.001421","url":null,"abstract":"Group A streptococcus (GAS) infections result in more than 500 000 deaths annually. Despite mounting evidence for airborne transmission of GAS, little is known about its stability in aerosol. Measurements of GAS airborne stability were carried out using the Controlled Electrodynamic Levitation and Extraction of Bioaerosols onto a Substrate (CELEBS) instrument. CELEBS measurements with two different isolates of GAS suggest that it is aerostable, with approximately 70 % of bacteria remaining viable after 20 min of levitation at 50 % relative humidity (RH), with lower survival as RH was reduced. GAS airborne viability loss was driven primarily by desiccation and efflorescence (i.e. salt crystallization), with high pH also potentially playing a role, given reduced survival in bicarbonate containing droplet compositions. At low enough RH for efflorescence to occur, a greater proportion of organic components in the droplet appeared to protect the bacteria from efflorescence. These first insights into the aerosol stability of GAS indicate that airborne transmission of these respiratory tract bacteria may occur, and that both the composition of the droplet containing the bacteria, and the RH of the air affect the duration of bacterial survival in this environment. Future studies will explore a broader range of droplet and air compositions and include a larger selection of GAS strains.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139099081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139. 与霍乱弧菌非 O1/ 非 O139 的 SXT 基因相连的抗生素耐药基因 floR 和 strA 的基因组和功能研究。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001424

Mousumi Saha, Agila Kumari Pragasam, Shashi Kumari, Jyoti Verma, Bhabatosh Das, Rupak K Bhadra

{"title":"Genomic and functional insights into antibiotic resistance genes floR and strA linked with the SXT element of Vibrio cholerae non-O1/non-O139.","authors":"Mousumi Saha, Agila Kumari Pragasam, Shashi Kumari, Jyoti Verma, Bhabatosh Das, Rupak K Bhadra","doi":"10.1099/mic.0.001424","DOIUrl":"10.1099/mic.0.001424","url":null,"abstract":"The emergence and spread of antibiotic-resistant bacterial pathogens are a critical public health concern across the globe. Mobile genetic elements (MGEs) play an important role in the horizontal acquisition of antimicrobial resistance genes (ARGs) in bacteria. In this study, we have decoded the whole genome sequences of multidrug-resistant Vibrio cholerae clinical isolates carrying the ARG-linked SXT, an integrative and conjugative element, in their large chromosomes. As in others, the SXT element has been found integrated into the 5'-end of the prfC gene (which encodes peptide chain release factor 3 involved in translational regulation) on the large chromosome of V. cholerae non-O1/non-O139 strains. Further, we demonstrate the functionality of SXT-linked floR and strAB genes, which confer resistance to chloramphenicol and streptomycin, respectively. The floR gene-encoded protein FloR belongs to the major facilitator superfamily efflux transporter containing 12 transmembrane domains (TMDs). Deletion analysis confirmed that even a single TMD of FloR is critical for the export function of chloramphenicol. The floR gene has two putative promoters, P1 and P2. Sequential deletions reveal that P2 is responsible for the expression of the floR. Deletion analysis of the N- and/or C-terminal coding regions of strA established their importance for conferring resistance against streptomycin. Interestingly, qPCR analysis of the floR and strA genes indicated that both of the genes are constitutively expressed in V. cholerae cells. Further, whole genome-based global phylogeography confirmed the presence of the integrative and conjugative element SXT in non-O1/non-O139 strains despite being non-multidrug resistant by lacking antimicrobial resistance (AMR) gene cassettes, which needs monitoring.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866021/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139099082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A toolbox for manipulating the genome of the major goat pathogen, Mycoplasma capricolum subsp. capripneumoniae. 操纵山羊主要病原体冠突支原体冠突肺炎亚种基因组的工具箱。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2024-01-01 DOI: 10.1099/mic.0.001423

Géraldine Gourgues, Lucía Manso-Silván, Catherine Chamberland, Pascal Sirand-Pugnet, François Thiaucourt, Alain Blanchard, Vincent Baby, Carole Lartigue

{"title":"A toolbox for manipulating the genome of the major goat pathogen, Mycoplasma capricolum subsp. capripneumoniae.","authors":"Géraldine Gourgues, Lucía Manso-Silván, Catherine Chamberland, Pascal Sirand-Pugnet, François Thiaucourt, Alain Blanchard, Vincent Baby, Carole Lartigue","doi":"10.1099/mic.0.001423","DOIUrl":"10.1099/mic.0.001423","url":null,"abstract":"Mycoplasma capricolum subspecies capripneumoniae (Mccp) is the causative agent of contagious caprine pleuropneumonia (CCPP), a devastating disease listed by the World Organisation for Animal Health (WOAH) as a notifiable disease and threatening goat production in Africa and Asia. Although a few commercial inactivated vaccines are available, they do not comply with WOAH standards and there are serious doubts regarding their efficacy. One of the limiting factors to comprehend the molecular pathogenesis of CCPP and develop improved vaccines has been the lack of tools for Mccp genome engineering. In this work, key synthetic biology techniques recently developed for closely related mycoplasmas were adapted to Mccp. CReasPy-Cloning was used to simultaneously clone and engineer the Mccp genome in yeast, prior to whole-genome transplantation into M. capricolum subsp. capricolum recipient cells. This approach was used to knock out an S41 serine protease gene recently identified as a potential virulence factor, leading to the generation of the first site-specific Mccp mutants. The Cre-lox recombination system was then applied to remove all DNA sequences added during genome engineering. Finally, the resulting unmarked S41 serine protease mutants were validated by whole-genome sequencing and their non-caseinolytic phenotype was confirmed by casein digestion assay on milk agar. The synthetic biology tools that have been successfully implemented in Mccp allow the addition and removal of genes and other genetic features for the construction of seamless targeted mutants at ease, which will pave the way for both the identification of key pathogenicity determinants of Mccp and the rational design of novel, improved vaccines for the control of CCPP.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10866025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139404998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbe Profile: The Lactobacillaceae. 微生物简介：乳酸菌科

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001414

Jens Walter, Paul W O'Toole

引用次数: 0

Microbial Primer: In vivo biofilm. 微生物入门：体内生物膜。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001407

Kendra P Rumbaugh, Thomas Bjarnsholt

引用次数: 0

Atomistic modelling and NMR studies reveal that gallium can target the ferric PQS uptake system in P. aeruginosa biofilms. 原子模型和核磁共振研究显示，镓可以靶向铜绿微囊藻生物膜中的铁质 PQS 吸收系统。

IF 2.8 4区生物学

Microbiology-Sgm Pub Date : 2023-12-01 DOI: 10.1099/mic.0.001422

Oliver J Hills, Isaac O K Noble, Alex Heyam, Andrew J Scott, James Smith, Helen F Chappell

{"title":"Atomistic modelling and NMR studies reveal that gallium can target the ferric PQS uptake system in P. aeruginosa biofilms.","authors":"Oliver J Hills, Isaac O K Noble, Alex Heyam, Andrew J Scott, James Smith, Helen F Chappell","doi":"10.1099/mic.0.001422","DOIUrl":"10.1099/mic.0.001422","url":null,"abstract":"Intravenous gallium nitrate therapy is a novel therapeutic strategy deployed to combat chronic Pseudomonas aeruginosa biofilm infections in the lungs of cystic fibrosis (CF) patients by interfering with iron (Fe3+) uptake. The therapy is a source of Ga3+, which competes with Fe3+ for siderophore binding, subsequently disrupting iron metabolism and inhibiting biofilm proliferation in vivo. It was recently demonstrated that the Pseudomonas quinolone signal (PQS) can chelate Fe3+ to assist in bacterial iron uptake. However, it is unknown whether exogenous gallium also targets [Fe(PQS)3] uptake, which, in turn, would extend the mechanism of gallium therapy beyond siderophore competition, potentially supporting use of the therapy against P. aeruginosa mutants deficient in siderophore uptake proteins. To that end, the thermodynamic feasibility of iron-for-gallium cation exchange into [Fe(PQS)3] was evaluated using quantum chemical density functional theory (DFT) modelling and verified experimentally using 1H nuclear magnetic resonance (NMR). We demonstrate here that Ga3+ can strongly bind to three PQS molecules and, furthermore, displace and substitute Fe3+ from the native chelate pocket within PQS complexes, through a Trojan horse mechanism, retaining the key structural features present within the native ferric complex. As such, [Fe(PQS)3] complexes, in addition to ferric-siderophore complexes, represent another target for gallium therapy.","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10765035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138806790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0