利用杂交测序技术对霍乱弧菌模式菌株进行基因组和表型比较研究。

IF 2.6 4区 生物学 Q3 MICROBIOLOGY
Øyvind M Lorentzen, Christina Bleis, Sören Abel
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引用次数: 0

摘要

下一代测序方法已成为研究细菌生物学和致病机理必不可少的方法,通常依赖于高质量的封闭基因组。在本研究中,我们利用混合测序方法组装了 C6706 的基因组,这是一种广泛使用的霍乱弧菌模式菌株。我们对基因组进行了人工编辑注释,通过将每个编码序列与 N16961(第一个测序的霍乱弧菌分离株和常用参考基因组)中的对应序列连接起来,提高了用户的可访问性。霍乱弧菌 C6706 和 N16961 的比较基因组分析发现了与关键生物功能相关的基因的多种遗传差异。为了确定这些遗传变异是否会导致表型差异,我们比较了与霍乱弧菌致病性相关的几种表型,如遗传稳定性、酸敏感性、生物膜形成和运动性。值得注意的是,与霍乱弧菌 C6706 相比,霍乱弧菌 N16961 的运动能力更强,生物膜形成更少。这些表型差异似乎是由菌株之间的法定量感应和环状二-GMP 信号通路的差异引起的。这项研究为霍乱弧菌生物膜的形成和运动的调控提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comparative genomic and phenotypic study of Vibrio cholerae model strains using hybrid sequencing.

Next-generation sequencing methods have become essential for studying bacterial biology and pathogenesis, often depending on high-quality, closed genomes. In this study, we utilized a hybrid sequencing approach to assemble the genome of C6706, a widely used Vibrio cholerae model strain. We present a manually curated annotation of the genome, enhancing user accessibility by linking each coding sequence to its counterpart in N16961, the first sequenced V. cholerae isolate and a commonly used reference genome. Comparative genomic analysis between V. cholerae C6706 and N16961 uncovered multiple genetic differences in genes associated with key biological functions. To determine whether these genetic variations result in phenotypic differences, we compared several phenotypes relevant to V. cholerae pathogenicity like genetic stability, acid sensitivity, biofilm formation and motility. Notably, V. cholerae N16961 exhibited greater motility and reduced biofilm formation compared to V. cholerae C6706. These phenotypic differences appear to be mediated by variations in quorum sensing and cyclic di-GMP signalling pathways between the strains. This study provides valuable insights into the regulation of biofilm formation and motility in V. cholerae.

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来源期刊
Microbiology-Sgm
Microbiology-Sgm 生物-微生物学
CiteScore
4.60
自引率
7.10%
发文量
132
审稿时长
3.0 months
期刊介绍: We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms. Topics include but are not limited to: Antimicrobials and antimicrobial resistance Bacteriology and parasitology Biochemistry and biophysics Biofilms and biological systems Biotechnology and bioremediation Cell biology and signalling Chemical biology Cross-disciplinary work Ecology and environmental microbiology Food microbiology Genetics Host–microbe interactions Microbial methods and techniques Microscopy and imaging Omics, including genomics, proteomics and metabolomics Physiology and metabolism Systems biology and synthetic biology The microbiome.
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