Veterinaria Mexico最新文献

{"title":"The welfare of pigs in rustic and technified production systems using the Welfare Quality protocols of pigs in Mexico: Validity of indicators of animal welfare as part of the sustainability criteria of pig production systems","authors":"N. Losada-Espinosa, M. T. Ortega, F. Galindo","doi":"10.21753/VMOA.4.4.521","DOIUrl":"https://doi.org/10.21753/VMOA.4.4.521","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Losada-Espinosa N,Trujillo Ortega ME, Galindo Maldonado FA. The welfare of pigs in rustic and technified production systems using the Welfare Quality protocols of pigs in Mexico: Validity of indicators of animal welfare as part of the sustainability criteria of pig production systems. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.521 . The Welfare Quality® (WQ) protocols have been developed as a tool for the assessment of farm animal welfare based on scientific evidence. Animal welfare (AW) is part of the sustainability criteria of livestock production. A study was carried out in four states of Central Mexico in seven rustic (rPS) and six technified (tPS) production systems using the WQ protocol with the objective of providing an initial approximation of the welfare of animals and to discuss the validity of indicators of AW. The results showed that the animals housed in rustic units presented better results in the Good Health category and with respect to the criterion expression of social behaviour, while the frequency of criteria concerning Positive emotional states was higher in animals in the technified units. In the changing context in which the farms operate, including changing agricultural policies, new environmental and food safety regulations, variability of climatic conditions, and volatility in prices of inputs and outputs, it is not only the attributes referring to productivity and efficiency that become relevant. It is concluded that the criteria related to the WQ principles of health and behaviour are sensitive to changes in the housing and management of pigs. The high occurrences of health and behaviour problems recorded in technified systems are an indicator of poor welfare. Figure 4 . Classification of rustic (RS) and technified (TS) production units within the welfare categories proposed by the Welfare Quality protocol.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.4.521","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47240776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico","authors":"M. M. Ramírez, R. Ponce, C. Ruíz, Graciela Castañeda Aceves, Ernesto Marín Flamand, G. Fariña, H. Álvarez","doi":"10.21753/VMOA.4.4.469","DOIUrl":"https://doi.org/10.21753/VMOA.4.4.469","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Turral Ramirez MM, Cordova Ponce R, Gonzalez Ruiz C, Castaneda Aceves G, Marin Flamand E, Garrido Farina G, Ramirez Alvarez H. First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.469 . The illegal trafficking of exotic birds such as parrots is now the third most lucrative business in the world and has been a problem for several years. As a result of this trafficking, there has been an increase in the emergence of exotic diseases. Herpesvirus is a pathogen of psittacines that has not been identified in Mexico to date. Through the use of polymerase chain reaction (PCR) assays and pathological analyses, the present study demonstrates the presence of herpesvirus in endemic and exotic psittacines in Mexico. The study was based on 50 birds, with clinical signs of herpervirus infection, and 45 clinically healthy birds. DNA samples were obtained from proctodaeal cells of the healthy birds, collected through the use of an enema; additionally, the liver, kidneys, brain, large intestines, and lungs of five birds exhibiting clinical signs suggestive of herpesvirus infection, were collected and fixed in buffered formalin for histopathological examination. PCR assays revealed herpesvirus infection in 8 clinically healthy birds, including four exotic species, and one endemic species. On necropsy, four of the five birds with clinical signs of herpesvirus infection showed lesions caused by herpesvirus, and the presence of intranuclear inclusion bodies in various tissues. Thus, these results demonstrate that there is a high risk of herpesvirus infection in endemic Mexican psittacines, which could significantly impact the health of these populations. Figure 5. a) Liver with mononuclear infiltrate (MNI) (100× magnification). The image shows the parenchyma at the interstitial level with a mononuclear inflammatory infiltrate characterized by the presence of lymphocytes (L), plasma cells (P), and macrophages (MO). b) Liver with necrosis and mononuclear infiltrate (40× magnification). Zones of coagulative necrosis (N) delimited by the inflammatory mononuclear infiltrate (arrow) are evident. c) Kidney with necrosis and mononuclear infiltrate (10× magnification). Renal parenchyma with extensive areas of coagulative necrosis (N) between the cortex and the medulla were observed. These areas were characterized by a marked loss of architecture and cellular detail and were delimited by mononuclear inflammatory cells (arrows). d) Necrotic intestine (100× magnification). The intestine developed severe coagulative necrosis (N) throughout the mucosa. Additionally, inflammatory infiltrates of mononuclear cells were observed near the basal membrane of the mucosa (arrows).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.4.469","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45759176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring lidia cattle with GPS-GPRS technology; a study on grazing behaviour and spatial distribution","authors":"Juan Manuel Santana Pérez, M. A. D. L. Varga, Juan García, V. G. Lacasa","doi":"10.21753/VMOA.4.4.405","DOIUrl":"https://doi.org/10.21753/VMOA.4.4.405","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Como citar este articulo: Lomillos Perez JM, Alonso de la Varga ME, Garcia JJ, Gaudioso Lacasa VR. Monitorizacion del ganado lidia con tecnologia GPS-GPRS; estudio del comportamiento en pastoreo y la distribucion espacial. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.405 . En los ultimos anos, el comportamiento del ganado vacuno en pastoreo ha sido poco  estudiado, en comparacion con el comportamiento en granja. Ademas, en determinadas razas criadas en sistemas extensivos, la presencia humana puede modificar o interrumpir sus pautas etologicas:  los animales se alejan de quien los observa. El uso de la tecnologia GPS-GPRS en la monitorizacion de bovinos explotados en regimen extensivo nos permite disponer de datos de interes en tiempo real; proporciona informacion sobre desplazamientos, distancias recorridas, territorio pastoreado, zonas mas frecuentadas, pautas de comportamiento, etc. En esta investigacion, se monitorizaron, mediante la implantacion de collares GPS, 21 vacas de diferentes edades, de tres ganaderias de la provincia de Salamanca pertenecientes a la raza bovina lidia. El estudio se llevo a cabo durante ocho meses, distribuimos a los animales en cercados de diferentes dimensiones y caracteristicas orograficas, y recogimos datos de su posicion geografica cada 15 minutos. Ademas de corroborar el buen funcionamiento de los dispositivos GPS, se establecio el area de campeo para cada animal: la media fue de 56 ha. Elaboramos un grafico de su ritmo circadiano con las distancias recorridas por horas, donde se observa una tendencia a iniciar la actividad diaria unas horas antes del amanecer, atenuar su actividad al anochecer y durante la noche, asi como mantener una fase de reposo nocturno de aproximadamente siete horas. Tambien analizamos la distancia diaria recorrida (3.15 km media/animal), donde encontramos diferencias sustanciales en funcion de la edad, la superficie/animal disponible en el cercado, el dia y la noche, y la epoca del ano. Nuestros resultados son valiosos para un mejor manejo de las superficies pastables con cercados en los que los animales utilicen toda la superficie disponible. Figura 3. Imagen del cercado 5 con las posiciones de los tres animales monitorizados. Rojo, amarillo y azul.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.4.405","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42724551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of biovar 6 in birds immunized against Gallibacterium anatis in poultry farms located in Sonora, Mexico.","authors":"R. Chávez, Ramón Miguel Molina Barrios, J. Chávez, Jorge Robles Mascareño, Jesus Escalante, Martín Acuña Yanes","doi":"10.21753/VMOA.4.3.389","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.389","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Osuna Chavez RF, Molina Barrios RM, Hernandez Chavez JF, Robles Mascareno J, Icedo Escalante JGA, Acuna Yanes M. First report of biovar 6 in birds immunized against Gallibacterium anatis in poultry farms located in Sonora, Mexico. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.389 . Identification of the predominant circulating biovars is important for controlling Gallibacterium anatis infections in poultry because efficient protection is based on the use of biovar-specific immunogens. Twenty-three isolates of Gallibacterium anatis were recovered from commercial laying hens with respiratory and reproductive clinical signs. Bacteria were identified as G. anatis based on morphology, biochemistry, and PCR testing. Biochemical analyses identified the isolates as biovar 6 which had not previously been reported in Sonora, Mexico. Therefore, the recent finding of biovar type 6 could explain clinical presentations of this disease in immunized commercial laying flocks in Sonora. Figure 1. PCR amplification gene, Gallibacterium anatis .","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.389","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44268250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine","authors":"Ana G. Serrato López, J. Montesinos, S. Arce","doi":"10.21753/VMOA.4.3.441","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.441","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Serrato Lopez AG, Montesinos Montesinos JJ, Anzaldua Arce SR. The endometrium as a source of mesenchymal stem cells in domestic animals and possible applications in veterinary medicine. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.441 . Mesenchymal stem cells (MSCs) have been isolated from the endometrium of humans, mice, cows, pigs and ewes. Typically, these cells are detected in the deep regions of the endometrium, closer to the union with the myometrium. MSCs possess characteristics such as clonogenicity and multipotentiality since they can differentiate in vitro into adipogenic, chondrogenic and osteogenic lineages. These cells can be induced to differentiate in vitro not only into the mesodermal lineage but also into the endodermal and ectodermal lineages. Therefore, MSCs show a great regenerative capacity for various organs and tissues, including the endometrium. Some advantages of endometrial MSCs compared with other MSC sources are their immune modulating activity, their ease of obtainment, and the amount of sample that may be collected. The study of endometrial MSCs in domestic animals is a new and promising field because increasing our understanding of the physiology and biology of these cells may lead to a better understanding of the physiopathology of reproductive diseases, and the development of treatment methods for infertility problems. In other veterinary medicine fields, MSCs can be used for the treatment of autoimmune diseases, cardiac affections, musculoskeletal and articular lesions, muscle degeneration, type 1 diabetes, urinary tract diseases, neurodegenerative processes and tumours. Finally, MSCs are also an important clinical tool for tissue engineering and regenerative medicine. The aim of this review is to present an updated outlook of the knowledge regarding endometrial MSCs and their possible applications in veterinary medicine. Figure 1: Immunoregulatory ability of MSCs. MSCs regulate the functions of NK cells, dendritic cells (DC) and T lymphocytes. The immunosuppressive effect may occur through the secretion of different factors or through cellular contact (black arrows). The former pathway involves TGFs, HGF, IL-10, PGE2, and HLA-G5, whereas the latter pathway involves the products of IDO enzyme activity, PD-L1, HLA-G1, ICAM-I and VCAM-I. Pro-inflammatory cytokines (IFN-?) secreted by NK cells and activated T lymphocytes favour the immunoregulatory activity of MSCs (dotted lines), because they increase or induce the secretion of molecules that regulate the functions of the distinct cellular components of the immune system. Modified from Montesinos et al, and Ma et al. 19,66","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.441","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48419714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conjugated linoleic acid supplementation does not improve boar semen quality and does not change its fatty acid profile","authors":"V. Zamora-Zamora, J. L. Figueroa-Velasco, J. L. Cordero-Mora, Rafael Nieto-Aquino, A. García-Contreras, M. T. Sánchez-Torres, S. Carrillo-Domínguez, J. A. Martínez-Aispuro","doi":"10.21753/VMOA.4.3.387","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.387","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Zamora-Zamora V, Figueroa-Velasco JL, Cordero-Mora JL, Nieto-Aquino R, Garcia-Contreras AC, Sanchez-Torres MT, Carrillo-Dominguez S, Martinez-Aispuro JA. Conjugated linoleic acid supplementation does not improve boar semen quality and does not change its fatty acid profile. Veterinaria Mexico OA. 2017;4(3). doi: 10.21753/vmoa.4.3.387 . The aim of this study was to evaluate the effects of adding conjugated linoleic acid (CLA) to boar diets on semen producion and characteristics, the fatty acid profile of spermatozoa, and on testicle and boar carcass parameters. Ten lean-type terminal-line (Yorkshire x Landrace x Petrain) adult boars were randomly assigned to one of two treatments: (1) a control diet and (2) the same diet with 1% CLA. The research was conducted in Mexico, and the animals were exposed to ambient temperatures betwen 13.5 and 26.3 °C. Boars were ejaculated twice per week a 12-week period to examine semen production and characteristics. The results suggest that supplementation of boar diets with CLA did not affect the spermatozoa fatty acid profile, semen volume, sperm concetration, viability, or motility, nor did it cause abnormal sperm morphology. Compared to controls, boar fed a CLA diet had a higher seminal pH and increased backfat thickness. In summary, this study indicates that CLA addition in boar diet does not change the semen characteristics of fatty acid profile of spermatozoa; however, it increases backfat thickness in sexually mature boars.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42673974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Geographic distribution of Desmodus rotundus in Mexico under current and future climate change scenarios: Implications for bovine paralytic rabies infection","authors":"H. Zarza, E. Martínez‐Meyer, G. Suzán, G. Ceballos","doi":"10.21753/VMOA.4.3.390","DOIUrl":"https://doi.org/10.21753/VMOA.4.3.390","url":null,"abstract":"Climate change may modify the spatial distribution of reservoirs hosting emerging and reemerging zoonotic pathogens, and forecasting these changes is essential for developing prevention and adaptation strategies. The most important reservoir of bovine paralytic rabies in tropical countries, is the vampire bat (Desmodus rotundus). In Mexico, the cattle industry loses more than $2.6 million US dollar, annually to this infectious disease. Therefore, we predicted the change in the distribution of D. rotundus due to future climate change scenarios, and examined the likely effect that the change in its distribution will have on paralytic rabies infections in Mexico. We used the correlative maximum entropy based model algorithm to predict the potential distribution of D. rotundus. Consistent with the literature, our results showed that temperature was the variable most highly associated with the current distribution of vampire bats. The highest concentration of bovine rabies was in Central and Southeastern Mexico, regions that also have high cattle population densities. Furthermore, our climatic envelope models predicted that by 2050–2070, D. rotundus will lose 20 % of its current distribution while the northern and central regions of Mexico will become suitable habitats for D. rotundus. Together, our study provides an advanced notice of the likely change in spatial patterns of D. rotundus and bovine paralytic rabies, and presents an important tool for strengthening the National Epidemiological Surveillance System and Monitoring programmes, useful for establishing holistic, long-term strategies to control this disease in Mexico.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.3.390","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46108121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa","authors":"C. Ramírez, S. M. G. Camacho, I. Ramírez, I. E. Verdugo, N. C. Campo, H. Moreno","doi":"10.21753/VMOA.4.2.369","DOIUrl":"https://doi.org/10.21753/VMOA.4.2.369","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Hernandez Ramirez CV, Gaxiola Camacho SM, Osuna Ramirez I, Enriquez Verdugo I, Castro del Campo N, Lopez Moreno HS. Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa. Veterinaria Mexico OA 2017;4(2).doi: 10.21753/vmoa.4.2.369 Domestic dogs transmit Leptospira spp. to humans, and determining the health risk that they represent is of paramount importance. To determine the seroprevalence and main risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa, we obtained serum samples from 165 dogs. The samples were stored at -40 °C and were analysed by the microbiology laboratory at Centro Nacional de Sanidad Animal using the leptospirosis microscopic agglutination test. Additionally, a survey was performed to identify epidemiological risk factors, and statistical inference was determined using chi-square test, odd ratios, and logistic regression with a statistical significance set at P < 0.05. The prevalence of Leptospira was 9 % (15/165), and we identified seven serovars: canicola 17 (46 %), icterohaemorrhagiae (40 %), bratislava (40 %), grippotyphosa (33 %), shermani (33 %), pyrogenes (20 %) and ballum (13 %). Based on our epidemiological survey, the risk factors associated with the detection of antibodies against Leptospira include the permanent habitation of pets in courtyards (OR = 4.6, P < 0.05) and presence of water stored in drums and basins (OR = 3.25, P < 0.05). The prevalence of leptospirosis in dogs indicates that the disease is present in the city of Culiacan and that leptospiral antibodies in dogs increase in poor sanitary conditions with stored water, which increases the potential risk of infection for both humans and animals. Figure 1. Frequency of observed serovars of Leptospira","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.2.369","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45255360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity","authors":"J. Prado, F. M. Palacios, R. C. Sedano, H. M. Larios","doi":"10.21753/VMOA.4.2.424","DOIUrl":"https://doi.org/10.21753/VMOA.4.2.424","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Cisneros Prado JL, Montiel Palacios F, Canseco Sedano R, Merchant Larios H. Ultrastructure of bovine preantral follicles: effect of the slaughterhouse aseptic process on follicular integrity. Veterinaria Mexico OA. 2017;4(2). doi: 10.21753/vmoa.4.2.424 . Ovaries from slaughtered cows are the most abundant source of preantral immature follicles for in vitro studies including maturation and in vitro fertilization. Integrity of immature follicles may be compromised by inadequate management during the collection and transport of the gonads, limiting the success of a correct evaluation. This study assessed the effect of aseptic and transport of bovine ovaries on the structural and ultrastructural integrity of immature follicles from the ovarian cortex. Ovaries from 20 different crossbred cows ( Bos taurus × B. indicus ) were randomly assigned to one of two aseptic and transport treatments: (1) the ovaries were washed once with 70 % ethanol for 10 s, rinsed twice with normal saline solution (OH + NSS), and then transported in NSS + 0.05 % gentamicin sulphate; or (2) the ovaries were washed three times with phosphate buffered saline (PBS) and transported in PBS + 0.05 % gentamicin sulphate. Samples of the ovarian cortex were obtained from each treatment group. Half of samples of each treatment was processed for histologic evaluation of follicular morphology and developmental stage using haematoxylin & eosin, and the other half was processed to analyse the ultrastructure of immature follicles using transmission electronic microscopy. The rates of good-quality follicles were 36.95 % and 88.6 % for OH + NSS and PBS, respectively. Histological analysis showed higher number of abnormal follicles in OH + NSS than in PBS treated ovaries (F 1,12 = 99.64, P = 0.0001). The proportions of morphological alterations were not significantly different between treatments (χ 2 1,7 = 7.72, P = 0.358). The ultrastructure of immature follicles from OH + NSS ovaries showed cell damage, revealed by abundant cell detritus around both, the oocyte and follicular cells. However, the presence of alterations was barely evident in both the cytoplasm and the nucleus of the granulosa cells. Figure 2. Semi-fine cuts of immature follicles present in bovine ovarian cortex treated with PBS (A, B, C; viewed at 40X) or OH+NSS (D, E, F; viewed at 40X).","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.2.424","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48487850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative evaluation of osteogenesis through infrared light: Pilot study","authors":"P. A. L. Mejía, Pablo Alfonso González Lomelín, H. L. Butron, R. D. Rubio, S. R. L. Hernández, I. Valdéz, V. Hernández","doi":"10.21753/VMOA.4.2.410","DOIUrl":"https://doi.org/10.21753/VMOA.4.2.410","url":null,"abstract":"Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Lomeli Mejia PA, Gonzalez Lomelin PA, Lecona Butron H, Dominguez Rubio R, Leon Hernandez SR, Luna Valdez IX, Dominguez Hernandez V. Quantitative evaluation of osteogenesis through infrared light. Pilot study. Veterinaria Mexico OA. 2017;4(2). doi: 10.21753/vmoa.4.2.410 . Different methods are available to evaluate the degree of bone healing. A good choice involves employing optical techniques with infrared light to eval-uate the progress of bone consolidation. Because infrared light is absorbed in liquids and reflected in solids, it is possible to assess bone consolidation progress using an incident light source and a coupled photo sensor. We used a 940-nm light source that is capable of reaching bone to determine the degree of bone consolidation. Five New Zealand White rabbits were used according to the NOM-062-ZOO99 standard. In each animal, a fracture was generated in the left tibia, and the fragments were fixed using an external fixator constructed with Kirschner nails and dental acrylic cement. Progress in bone consolidation was evaluated at days 7, 23, and 34 after surgery. A linear dependence was observed between the days elapsed and the reflection of the infrared light. Figure 2. Reduction of tibia by external fixators made with Kirschner nails 0.045 inch in diameter and methylmethacrylate cement.","PeriodicalId":49387,"journal":{"name":"Veterinaria Mexico","volume":null,"pages":null},"PeriodicalIF":0.2,"publicationDate":"2017-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.21753/VMOA.4.2.410","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45824471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}