首次在墨西哥地方性和外来鹦鹉中发现疱疹病毒感染

IF 0.2 4区 农林科学 Q2 Veterinary
M. M. Ramírez, R. Ponce, C. Ruíz, Graciela Castañeda Aceves, Ernesto Marín Flamand, G. Fariña, H. Álvarez
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引用次数: 0

摘要

墨西哥兽医OA ISSN:2448-6760引用如下:Turral Ramirez MM、Cordova Ponce R、Gonzalez Ruiz C、Castaneda Aceves G、Marin Flamand E、Garrido Farina G、Ramirez Alvarez H.首次在墨西哥地方病和外来鹦鹉螺中发现疱疹病毒感染。兽医墨西哥OA。2017年;4(4)。doi:10.21753/vmoa.4.4.469.非法贩运鹦鹉等珍禽现在是世界上利润第三高的行业,多年来一直是一个问题。由于这种贩运,外来疾病的出现有所增加。疱疹病毒是一种至今尚未在墨西哥发现的鹦鹉热病原体。通过聚合酶链式反应(PCR)检测和病理分析,本研究证明了墨西哥地方性和外来鹦鹉螺中存在疱疹病毒。这项研究基于50只有疱疹病毒感染临床症状的鸟类和45只临床健康的鸟类。DNA样本是从健康鸟类的直肠细胞中获得的,通过灌肠法收集;此外,收集了五只表现出疱疹病毒感染临床症状的鸟类的肝脏、肾脏、大脑、大肠和肺部,并将其固定在缓冲福尔马林中进行组织病理学检查。PCR检测显示8只临床健康鸟类感染疱疹病毒,其中包括4种外来物种和1种特有物种。尸检时,五只有疱疹病毒感染临床症状的鸟中有四只表现出疱疹病毒引起的病变,并且在各种组织中存在核内包涵体。因此,这些结果表明,墨西哥地方性鹦鹉螺感染疱疹病毒的风险很高,这可能会严重影响这些人群的健康。图5。a) 肝脏有单核细胞浸润(MNI)(放大100倍)。图像显示间质水平的薄壁组织具有单核炎症浸润,其特征是存在淋巴细胞(L)、浆细胞(P)和巨噬细胞(MO)。b) 肝脏坏死和单核细胞浸润(放大40倍)。由炎性单核细胞浸润(箭头)界定的凝固性坏死(N)区是明显的。c) 肾坏死和单核细胞浸润(放大10倍)。肾实质在皮质和髓质之间有大面积的凝固性坏死(N)。这些区域的特征是结构和细胞细节的显著丧失,并由单核炎症细胞界定(箭头)。d) 坏死肠(放大100倍)。肠粘膜出现严重的凝固性坏死(N)。此外,在粘膜基膜附近观察到单核细胞的炎症浸润(箭头)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico
Veterinaria Mexico OA ISSN: 2448-6760 Cite this as: Turral Ramirez MM, Cordova Ponce R, Gonzalez Ruiz C, Castaneda Aceves G, Marin Flamand E, Garrido Farina G, Ramirez Alvarez H. First identification of Herpesvirus infections among endemic and exotic psittacines in Mexico. Veterinaria Mexico OA. 2017;4(4). doi: 10.21753/vmoa.4.4.469 . The illegal trafficking of exotic birds such as parrots is now the third most lucrative business in the world and has been a problem for several years. As a result of this trafficking, there has been an increase in the emergence of exotic diseases. Herpesvirus is a pathogen of psittacines that has not been identified in Mexico to date. Through the use of polymerase chain reaction (PCR) assays and pathological analyses, the present study demonstrates the presence of herpesvirus in endemic and exotic psittacines in Mexico. The study was based on 50 birds, with clinical signs of herpervirus infection, and 45 clinically healthy birds. DNA samples were obtained from proctodaeal cells of the healthy birds, collected through the use of an enema; additionally, the liver, kidneys, brain, large intestines, and lungs of five birds exhibiting clinical signs suggestive of herpesvirus infection, were collected and fixed in buffered formalin for histopathological examination. PCR assays revealed herpesvirus infection in 8 clinically healthy birds, including four exotic species, and one endemic species. On necropsy, four of the five birds with clinical signs of herpesvirus infection showed lesions caused by herpesvirus, and the presence of intranuclear inclusion bodies in various tissues. Thus, these results demonstrate that there is a high risk of herpesvirus infection in endemic Mexican psittacines, which could significantly impact the health of these populations. Figure 5. a) Liver with mononuclear infiltrate (MNI) (100× magnification). The image shows the parenchyma at the interstitial level with a mononuclear inflammatory infiltrate characterized by the presence of lymphocytes (L), plasma cells (P), and macrophages (MO). b) Liver with necrosis and mononuclear infiltrate (40× magnification). Zones of coagulative necrosis (N) delimited by the inflammatory mononuclear infiltrate (arrow) are evident. c) Kidney with necrosis and mononuclear infiltrate (10× magnification). Renal parenchyma with extensive areas of coagulative necrosis (N) between the cortex and the medulla were observed. These areas were characterized by a marked loss of architecture and cellular detail and were delimited by mononuclear inflammatory cells (arrows). d) Necrotic intestine (100× magnification). The intestine developed severe coagulative necrosis (N) throughout the mucosa. Additionally, inflammatory infiltrates of mononuclear cells were observed near the basal membrane of the mucosa (arrows).
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来源期刊
Veterinaria Mexico
Veterinaria Mexico VETERINARY SCIENCES-
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期刊介绍: Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).
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