Viruses-Basel最新文献_第9页

Kinetic Landscape of Single Virus-like Particles Highlights the Efficacy of SARS-CoV-2 Internalization. 单个病毒样颗粒的动力学图谱凸显了 SARS-CoV-2 的内化功效。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-22 DOI: 10.3390/v16081341

Aleksandar Atemin, Aneliya Ivanova, Wiley Peppel, Rumen Stamatov, Rodrigo Gallegos, Haley Durden, Sonya Uzunova, Michael D Vershinin, Saveez Saffarian, Stoyno S Stoynov

{"title":"Kinetic Landscape of Single Virus-like Particles Highlights the Efficacy of SARS-CoV-2 Internalization.","authors":"Aleksandar Atemin, Aneliya Ivanova, Wiley Peppel, Rumen Stamatov, Rodrigo Gallegos, Haley Durden, Sonya Uzunova, Michael D Vershinin, Saveez Saffarian, Stoyno S Stoynov","doi":"10.3390/v16081341","DOIUrl":"10.3390/v16081341","url":null,"abstract":"The efficiency of virus internalization into target cells is a major determinant of infectivity. SARS-CoV-2 internalization occurs via S-protein-mediated cell binding followed either by direct fusion with the plasma membrane or endocytosis and subsequent fusion with the endosomal membrane. Despite the crucial role of virus internalization, the precise kinetics of the processes involved remains elusive. We developed a pipeline, which combines live-cell microscopy and advanced image analysis, for measuring the rates of multiple internalization-associated molecular events of single SARS-CoV-2-virus-like particles (VLPs), including endosome ingression and pH change. Our live-cell imaging experiments demonstrate that only a few minutes after binding to the plasma membrane, VLPs ingress into RAP5-negative endosomes via dynamin-dependent scission. Less than two minutes later, VLP speed increases in parallel with a pH drop below 5, yet these two events are not interrelated. By co-imaging fluorescently labeled nucleocapsid proteins, we show that nucleocapsid release occurs with similar kinetics to VLP acidification. Neither Omicron mutations nor abrogation of the S protein polybasic cleavage site affected the rate of VLP internalization, indicating that they do not confer any significant advantages or disadvantages during this process. Finally, we observe that VLP internalization occurs two to three times faster in VeroE6 than in A549 cells, which may contribute to the greater susceptibility of the former cell line to SARS-CoV-2 infection. Taken together, our precise measurements of the kinetics of VLP internalization-associated processes shed light on their contribution to the effectiveness of SARS-CoV-2 propagation in cells.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359012/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIV-MTB Co-Infection Reduces CD4+ T Cells and Affects Granuloma Integrity. HIV-MTB 合并感染会减少 CD4+ T 细胞并影响肉芽肿的完整性。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081335

Suyue Huang, Maoying Liu, Hui Zhang, Wei Song, Wenjuan Guo, Yanling Feng, Xin Ma, Xia Shi, Jianjian Liu, Li Liu, Tangkai Qi, Zhenyan Wang, Bo Yan, Yinzhong Shen

{"title":"HIV-MTB Co-Infection Reduces CD4+ T Cells and Affects Granuloma Integrity.","authors":"Suyue Huang, Maoying Liu, Hui Zhang, Wei Song, Wenjuan Guo, Yanling Feng, Xin Ma, Xia Shi, Jianjian Liu, Li Liu, Tangkai Qi, Zhenyan Wang, Bo Yan, Yinzhong Shen","doi":"10.3390/v16081335","DOIUrl":"10.3390/v16081335","url":null,"abstract":"Granuloma is a crucial pathological feature of tuberculosis (TB). The relationship between CD4+ T cells in both peripheral blood and granulomatous tissue, and the integrity of granulomas in Human Immunodeficiency Virus (HIV)-MTB co-infection, remains unexplored. This study collected biopsy specimens from 102 TB patients (53 with HIV-MTB co-infection and 49 only with TB). Hematoxylin and eosin (HE) staining and immunohistochemical staining were performed, followed by microscopic examination of the integrity of tuberculous granulomas. Through statistical analysis of peripheral blood CD4+ T cell counts, tissue CD4+ T cell proportion, and the integrity of granulomas, it was observed that HIV infection leads to poor formation of tuberculous granulomas. Peripheral blood CD4+ T cell counts were positively correlated with granuloma integrity, and there was a similar positive correlation between tissue CD4+ T cell proportions and granuloma integrity. Additionally, a positive correlation was found between peripheral blood CD4+ T cell counts and the proportion of CD4+ T cells in granuloma tissues. Therefore, HIV infection could impact the morphology and structure of tuberculous granulomas, with a reduced proportion of both peripheral blood and tissue CD4+ T lymphocytes.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11360352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Antibodies against Feline Morbillivirus by Recombinant Matrix Enzyme-Linked Immunosorbent Assay. 用重组基质酶联免疫吸附试验检测猫莫比尔病毒抗体。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081339

Surangkanang Chaiyasak, Chutchai Piewbang, Jadsada Ratthanophart, Navapon Techakriengkrai, Kittipong Rattanaporn, Somporn Techangamsuwan

{"title":"Detection of Antibodies against Feline Morbillivirus by Recombinant Matrix Enzyme-Linked Immunosorbent Assay.","authors":"Surangkanang Chaiyasak, Chutchai Piewbang, Jadsada Ratthanophart, Navapon Techakriengkrai, Kittipong Rattanaporn, Somporn Techangamsuwan","doi":"10.3390/v16081339","DOIUrl":"10.3390/v16081339","url":null,"abstract":"Feline morbillivirus (FeMV) has been associated with feline health, although its exact role in pathogenesis is still debated. In this study, an indirect enzyme-linked immunosorbent assay (i-ELISA) targeting a recombinant matrix protein of FeMV (rFeMV-M) was developed and assessed in comparison to a Western blotting (WB) assay. The i-ELISA was evaluated using blood samples from 136 cats that were additionally tested with real-time reverse-transcription PCR (RT-qPCR). The i-ELISA exhibited a sensitivity of 90.1%, specificity of 75.6%, positive predictive value of 88.2%, and negative predictive value of 79.1%. The agreement between i-ELISA and WB analyses was substantial (a κ coefficient of 0.664 with a 95% confidence interval of 0.529 to 0.799). Within the study group, 68.4% (93/136) of the cats were serologically positive in the i-ELISA and 66.9% (91/136) in the WB assay, with 11.8% (11/93) of false positivity with the i-ELISA. However, only 8.1% (11/136) of the cats tested positive for FeMV using RT-qPCR (p < 0.001). The developed i-ELISA proved effective in identifying FeMV-infected cats and indicated the prevalence of FeMV exposure. Combining FeMV antibody detection through i-ELISA with FeMV RT-qPCR could offer a comprehensive method to determine and monitor FeMV infection status. Nevertheless, this assay still requires refinement due to a significant number of false positive results, which can lead to the misdiagnosis of cats without antibodies as having antibodies. This study also provided the first evidence of seroprevalence against FeMV among cat populations in Thailand, contributing valuable insights into the geographic distribution and prevalence of this virus.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in Clinico-Epidemiological Profile and Outcomes of Patients with HIV-Associated Cryptococcal Meningitis in Shanghai, China, 2013-2023. 2013-2023 年中国上海艾滋病相关隐球菌脑膜炎患者的临床流行病学概况和预后趋势。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081333

Zihui Zhao, Wei Song, Li Liu, Tangkai Qi, Zhenyan Wang, Yang Tang, Jianjun Sun, Shuibao Xu, Junyang Yang, Jiangrong Wang, Jun Chen, Renfang Zhang, Yinzhong Shen

{"title":"Trends in Clinico-Epidemiological Profile and Outcomes of Patients with HIV-Associated Cryptococcal Meningitis in Shanghai, China, 2013-2023.","authors":"Zihui Zhao, Wei Song, Li Liu, Tangkai Qi, Zhenyan Wang, Yang Tang, Jianjun Sun, Shuibao Xu, Junyang Yang, Jiangrong Wang, Jun Chen, Renfang Zhang, Yinzhong Shen","doi":"10.3390/v16081333","DOIUrl":"10.3390/v16081333","url":null,"abstract":"The study aimed to analyze changes in the clinical and epidemiological aspects of HIV-associated cryptococcal meningitis (CM) patients and to identify factors influencing their prognosis. Clinical data of patients with HIV-associated CM treated in Shanghai, China between 2013 and 2023 were collected. This study included 279 cases, 2.89% of AIDS patients, showing a yearly decrease in CM prevalence among AIDS patients (p < 0.001). Overall mortality was 10.39% with rates declining from a 2013 peak of 15.38% to 0% in 2023 despite no significant temporal pattern (p = 0.265). Diagnosis took an average of 18 ± 1 days post-symptoms, and admission CD4 counts averaged 29.2 ± 2.5 cells/μL, hinting at a non-significant decline. Frequent symptoms included fever (62.4%), headache (61.6%), fatigue (44.1%), and appetite loss (39.8%), with younger patients more likely to initially show signs of meningeal irritation. Logistic regression analysis underscored the prognostic importance of cerebrospinal fluid (CSF) white blood cell (WBC) count and procalcitonin levels. Over the decade spanning from 2013 to 2023, the incidence and mortality rates of CM among AIDS patients exhibited a downward trend. The average duration from the onset of CM to confirmation of diagnosis remained prolonged. CSF WBC count and procalcitonin levels were associated with unfavorable outcomes.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359124/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Update on Hepatitis C Vaccine: Results and Challenges. 丙型肝炎疫苗的最新进展：成果与挑战。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081337

Anna Rosa Garbuglia, Silvia Pauciullo, Verdiana Zulian, Paola Del Porto

{"title":"Update on Hepatitis C Vaccine: Results and Challenges.","authors":"Anna Rosa Garbuglia, Silvia Pauciullo, Verdiana Zulian, Paola Del Porto","doi":"10.3390/v16081337","DOIUrl":"10.3390/v16081337","url":null,"abstract":"Therapy against the Hepatitis C virus (HCV) has significantly improved with the introduction of direct-acting antiviral drugs (DAAs), achieving over 95% sustained virological response (SVR). Despite this, the development of an effective anti-HCV vaccine remains a critical challenge due to the low number of patients treated with DAAs and the occurrence of HCV reinfections in high-risk groups. Current vaccine strategies aim to stimulate either B-cell or T-cell responses. Vaccines based on E1 and E2 proteins can elicit broad cross-neutralizing antibodies against all major HCV genotypes, though with varying efficiencies and without full protection against infection. In humans, the neutralizing antibodies induced by such vaccines mainly target the AR3 region, but their levels are generally insufficient for broad neutralization. Various HCV proteins expressed through different viral vectors have been utilized to elicit T cell immune responses, showing sustained expansion of HCV-specific effector memory T cells and improved proliferation and polyfunctionality of memory T cells over time. However, despite these advancements, the frequency and effectiveness of T-cell responses remain limited.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predominance of Dengue Virus Serotype-1/Genotype-I in Eastern and Southeastern Ethiopia. 埃塞俄比亚东部和东南部登革热病毒血清型-1/基因型-I的主要分布情况。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081334

Mesfin Mengesha Tsegaye, Adamu Tayachew Mekonnen, Daniel Tsega Gebretsion, Tesfaye Gelanew, Dawit Hailu Alemayehu, Dessalegn Abeje Tefera, Tamirayehu Seyoum Woldemichael, Bethlehem Adnew Getaneh, Eleni Kidane Abera, Gadissa Gutema Jebessa, Asefa Konde Korkiso, Mengistu Biru Tessema, Admikew Agunie Asfaw, Yoseph Asrat Temre, Mesfin Wossen, Anne Piantadosi, Huachen Zhu, Alemseged Abdissa, Adane Mihret, Andargachew Mulu

{"title":"Predominance of Dengue Virus Serotype-1/Genotype-I in Eastern and Southeastern Ethiopia.","authors":"Mesfin Mengesha Tsegaye, Adamu Tayachew Mekonnen, Daniel Tsega Gebretsion, Tesfaye Gelanew, Dawit Hailu Alemayehu, Dessalegn Abeje Tefera, Tamirayehu Seyoum Woldemichael, Bethlehem Adnew Getaneh, Eleni Kidane Abera, Gadissa Gutema Jebessa, Asefa Konde Korkiso, Mengistu Biru Tessema, Admikew Agunie Asfaw, Yoseph Asrat Temre, Mesfin Wossen, Anne Piantadosi, Huachen Zhu, Alemseged Abdissa, Adane Mihret, Andargachew Mulu","doi":"10.3390/v16081334","DOIUrl":"10.3390/v16081334","url":null,"abstract":"We determined the dengue virus (DENV) serotypes and genotypes in archived serum samples that were collected during the 2014-2016 and 2021 dengue outbreaks in Dire Dawa City and the Somali region in Ethiopia. DENV serotype 1 (DENV-1) was predominant followed by DENV serotype 2 (DENV-2). Thirteen of the DENV-1 strains were assigned to Genotype-I, while the remaining two were found to be Genotype-III. All three DENV-2 strains were assigned the Cosmopolitan Genotype. The DENV strains responsible for the outbreaks are genetically closely related to the DENV strains that circulated in neighboring and Asian countries. The findings also showed continued local transmission of a monophyletic lineage and a co-circulation of DENV-1 and DENV-2 during the outbreaks. There is a need to strengthen DENV genomic surveillance capacity for the early detection of circulating serotypes, and prevent devastating consequences of future outbreaks due to the co-circulation of different serotypes.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Phage Spray on Hatchability and Chick Quality of Eggs Contaminated with Salmonella Typhimurium. 噬菌体喷雾对受鼠伤寒沙门氏菌污染鸡蛋的孵化率和雏鸡质量的影响

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081338

Leping Wang, Qinting Dong, Kunping Tang, Kaiou Han, Huili Bai, Yangyan Yin, Changting Li, Chunxia Ma, Ling Teng, Jun Li, Yu Gong, Yuying Liao, Hao Peng, Xiaoye Wang

{"title":"Effect of Phage Spray on Hatchability and Chick Quality of Eggs Contaminated with Salmonella Typhimurium.","authors":"Leping Wang, Qinting Dong, Kunping Tang, Kaiou Han, Huili Bai, Yangyan Yin, Changting Li, Chunxia Ma, Ling Teng, Jun Li, Yu Gong, Yuying Liao, Hao Peng, Xiaoye Wang","doi":"10.3390/v16081338","DOIUrl":"10.3390/v16081338","url":null,"abstract":"Salmonella Typhimurium (S. Typhimurium) contamination poses a significant challenge to breeder egg hatchability and chick health, necessitating the exploration of alternative disinfection methods. This study investigates the potential of phage vB_SPuM_SP02 (SP02) as a novel disinfectant for breeder eggs contaminated with S. Typhimurium SM022. Phage SP02 was isolated from poultry farm effluent and characterized for morphology, biological properties, and genome properties. Experimental groups of specific pathogen-free (SPF) eggs were treated with Salmonella and phage SP02, and efficacy was assessed through hatching rates, chick survival, weight, Salmonella load, immune organ indices, and intestinal flora. Phage treatment effectively eradicated Salmonella contamination on eggshells within 12 h, resulting in increased hatching and survival rates compared to controls. Furthermore, phage treatment mitigated weight loss and tissue Salmonella load in chicks without causing immune organ damage while reducing Salmonella spp. abundance in the intestinal tract. This study demonstrates the potential of phage SP02 as an eco-friendly and efficient disinfectant for S. Typhimurium-contaminated breeder eggs, offering promising prospects for practical application in poultry production.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of PQBP1 in Pathogen Recognition-Impact on Innate Immunity. PQBP1 在病原体识别中的作用--对先天免疫的影响

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081340

Lukas Wiench, Domenico Rizzo, Zora Sinay, Zsófia Nacsa, Nina V Fuchs, Renate König

{"title":"Role of PQBP1 in Pathogen Recognition-Impact on Innate Immunity.","authors":"Lukas Wiench, Domenico Rizzo, Zora Sinay, Zsófia Nacsa, Nina V Fuchs, Renate König","doi":"10.3390/v16081340","DOIUrl":"10.3390/v16081340","url":null,"abstract":"The intrinsically disordered polyglutamine-binding protein 1 (PQBP1) has been linked to various cellular processes including transcription, alternative splicing, translation and innate immunity. Mutations in PQBP1 are causative for neurodevelopmental conditions collectively termed as the Renpenning syndrome spectrum. Intriguingly, cells of Renpenning syndrome patients exhibit a reduced innate immune response against human immunodeficiency virus 1 (HIV-1). PQBP1 is responsible for the initiation of a two-step recognition process of HIV-1 reverse-transcribed DNA products, ensuring a type 1 interferon response. Recent investigations revealed that PQBP1 also binds to the p17 protein of avian reovirus (ARV) and is affected by the ORF52 of Kaposi's sarcoma-associated herpesvirus (KSHV), possibly also playing a role in the innate immune response towards these RNA- and DNA-viruses. Moreover, PQBP1-mediated microglia activation in the context of tauopathies has been reported, highlighting the role of PQBP1 in sensing exogenous pathogenic species and innate immune response in the central nervous system. Its unstructured nature, the promiscuous binding of various proteins and its presence in various tissues indicate the versatile roles of PQBP1 in cellular regulation. Here, we systematically review the available data on the structure of PQBP1 and its cellular functions and interactome, as well as possible implications for innate immune responses and neurodegenerative disorders.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11360342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bunyamwera Virus Infection of Wolbachia-Carrying Aedes aegypti Mosquitoes Reduces Wolbachia Density. 布尼亚姆韦拉病毒感染携带沃尔巴克氏体的埃及伊蚊会降低沃尔巴克氏体的密度。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-21 DOI: 10.3390/v16081336

Daniella A Lefteri, Stephanie M Rainey, Shivan M Murdochy, Steven P Sinkins

{"title":"Bunyamwera Virus Infection of Wolbachia-Carrying Aedes aegypti Mosquitoes Reduces Wolbachia Density.","authors":"Daniella A Lefteri, Stephanie M Rainey, Shivan M Murdochy, Steven P Sinkins","doi":"10.3390/v16081336","DOIUrl":"10.3390/v16081336","url":null,"abstract":"Wolbachia symbionts introduced into Aedes mosquitoes provide a highly effective dengue virus transmission control strategy, increasingly utilised in many countries in an attempt to reduce disease burden. Whilst highly effective against dengue and other positive-sense RNA viruses, it remains unclear how effective Wolbachia is against negative-sense RNA viruses. Therefore, the effect of Wolbachia on Bunyamwera virus (BUNV) infection in Aedes aegypti was investigated using wMel and wAlbB, two strains currently used in Wolbachia releases for dengue control, as well as wAu, a strain that typically persists at a high density and is an extremely efficient blocker of positive-sense viruses. Wolbachia was found to reduce BUNV infection in vitro but not in vivo. Instead, BUNV caused significant impacts on density of all three Wolbachia strains following infection of Ae. aegypti mosquitoes. The ability of Wolbachia to successfully persist within the mosquito and block virus transmission is partially dependent on its intracellular density. However, reduction in Wolbachia density was not observed in offspring of infected mothers. This could be due in part to a lack of transovarial transmission of BUNV observed. The results highlight the importance of understanding the complex interactions between multiple arboviruses, mosquitoes and Wolbachia in natural environments, the impact this can have on maintaining protection against diseases, and the necessity for monitoring Wolbachia prevalence at release sites.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11360823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Inflammatory and Thyroid Hormone Levels Based on Hepatitis A and B Virus Immunity Status: Age and Sex Stratification. 基于甲型肝炎和乙型肝炎病毒免疫状态的炎症和甲状腺激素水平分析：年龄和性别分层。

IF 3.8 3区医学

Viruses-Basel Pub Date : 2024-08-20 DOI: 10.3390/v16081329

Hyeokjun Yun, Jae-Sik Jeon, Jae Kyung Kim

{"title":"Analysis of Inflammatory and Thyroid Hormone Levels Based on Hepatitis A and B Virus Immunity Status: Age and Sex Stratification.","authors":"Hyeokjun Yun, Jae-Sik Jeon, Jae Kyung Kim","doi":"10.3390/v16081329","DOIUrl":"10.3390/v16081329","url":null,"abstract":"This study investigated the potential associations between hepatitis virus antibody status and thyroid and inflammatory function. The C-reactive protein (CRP), thyroid-stimulating hormone (TSH), and free thyroxine (FT4) levels were measured in individuals with and without antibodies to the hepatitis A virus (HAV) and hepatitis B virus (HBV). Participants were stratified by age, sex, and HAV/HBV antibody status. Participants with and without antibodies to HAV and HBV had normal CRP, TSH, and FT4 levels. However, notable discrepancies were observed in FT4 levels among participants with HAV antibodies and in CRP and FT4 levels among those with both HAV and HBV antibodies, suggesting potential associations between viral immunity and thyroid function, especially in younger participants. Significant variations in thyroid hormone levels were noted when the sample was stratified by sex and HAV and HBV antibody status, indicating that the association between antibody status and thyroid hormone levels varied by sex. This study underscores the need for further research on the effect of viral immunity on inflammatory parameters and thyroid hormone levels.","PeriodicalId":49328,"journal":{"name":"Viruses-Basel","volume":null,"pages":null},"PeriodicalIF":3.8,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11358917/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142114004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0