Human Cell最新文献_第4页

Mesenchymal stem cells and their extracellular vesicles in bone and joint diseases: targeting the NLRP3 inflammasome. 间充质干细胞及其细胞外囊泡在骨与关节疾病中的作用：靶向 NLRP3 炎症小体。

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-10 DOI: 10.1007/s13577-024-01101-x

Shuangshuang Xu, Ying Zhang, Zejun Zheng, Jinmeng Sun, Yanan Wei, Gang Ding

{"title":"Mesenchymal stem cells and their extracellular vesicles in bone and joint diseases: targeting the NLRP3 inflammasome.","authors":"Shuangshuang Xu, Ying Zhang, Zejun Zheng, Jinmeng Sun, Yanan Wei, Gang Ding","doi":"10.1007/s13577-024-01101-x","DOIUrl":"10.1007/s13577-024-01101-x","url":null,"abstract":"The nucleotide-binding oligomerization domain-like-receptor family pyrin domain-containing 3 (NLRP3) inflammasome is a cytosolic multi-subunit protein complex, and recent studies have demonstrated the vital role of the NLRP3 inflammasome in the pathological and physiological conditions, which cleaves gasdermin D to induce inflammatory cell death called pyroptosis and mediates the release of interleukin-1 beta and interleukin-18 in response to microbial infection or cellular injury. Over-activation of the NLRP3 inflammasome is associated with the pathogenesis of many disorders affecting bone and joints, including gouty arthritis, osteoarthritis, rheumatoid arthritis, osteoporosis, and periodontitis. Moreover, mesenchymal stem cells (MSCs) have been discovered to facilitate the inhibition of NLRP3 and maybe ideal for treating bone and joint diseases. In this review, we implicate the structure and activation of the NLRP3 inflammasome along with the detail on the involvement of NLRP3 inflammasome in bone and joint diseases pathology. In addition, we focused on MSCs and MSC-extracellular vesicles targeting NLRP3 inflammasomes in bone and joint diseases. Finally, the existing problems and future direction are also discussed.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanofiber-based delivery of evodiamine impedes malignant properties of intrahepatic cholangiocarcinoma cells by targeting HDAC4 and restoring TPM1 transcription. 通过靶向 HDAC4 和恢复 TPM1 转录，以纳米纤维为基础的 evodiamine 递送抑制了肝内胆管癌细胞的恶性特性。

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-29 DOI: 10.1007/s13577-024-01105-7

Rui Zou, Yiyao Wang, Yaoqing Cai, Zhenming Xing, Yongfu Shao, Duo Li, Chunchun Qi

{"title":"Nanofiber-based delivery of evodiamine impedes malignant properties of intrahepatic cholangiocarcinoma cells by targeting HDAC4 and restoring TPM1 transcription.","authors":"Rui Zou, Yiyao Wang, Yaoqing Cai, Zhenming Xing, Yongfu Shao, Duo Li, Chunchun Qi","doi":"10.1007/s13577-024-01105-7","DOIUrl":"10.1007/s13577-024-01105-7","url":null,"abstract":"The electrospun nanofiber system is correlated with high efficacy of drug delivery. This study aims to investigate the effect of nanofiber-based delivery of evodiamine, an indole alkaloid derived from Rutaceae plants Evodia rutaecarpa (Juss.) Benth, on intrahepatic cholangiocarcinoma (ICC), as well as to explore the molecular mechanisms. An electrospun nanofiber system carrying evodiamine was generated. Compared to evodiamine treatment alone, the nano-evodiamine exhibited more pronounced effects on suppressing proliferation, colony formation, invasiveness, migration, apoptosis resistance, cell cycle progression, and in vivo tumorigenesis of two ICC cell lines (HUCC-T1 and RBE). ICC cells exhibited increased expression of histone deacetylase 4 (HDAC4) while decreased tropomyosin 1 (TPM1). HDAC4 suppressed TPM1 expression by removing H3K9ac modifications from its promoter. Nano-evodiamine reduced HDAC4 protein levels in ICC cells, thus promoting transcription and expression of TPM1. Either overexpression of HDAC4 or downregulation of TPM1 negated the tumor-suppressive effects of nano-evodiamine. Collectively, this study demonstrates that the electrospun nanofiber system enhances the efficiency of evodiamine. Additionally, evodiamine suppresses the malignant properties of ICC cells. The findings may provide fresh insights into the application of electrospun nanofiber system for drug delivery and the effects of evodiamine on tumor suppression.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141789611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential protumor function of CD74 in clear cell renal cell carcinoma. CD74在透明细胞肾细胞癌中的潜在原癌功能

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-30 DOI: 10.1007/s13577-024-01110-w

Ayano Ezaki, Hiromu Yano, Cheng Pan, Yukio Fujiwara, Toshiki Anami, Yuki Ibe, Takanobu Motoshima, Junji Yatsuda, Shigeyuki Esumi, Yuji Miura, Tomomi Kamba, Yoshihiro Komohara

{"title":"Potential protumor function of CD74 in clear cell renal cell carcinoma.","authors":"Ayano Ezaki, Hiromu Yano, Cheng Pan, Yukio Fujiwara, Toshiki Anami, Yuki Ibe, Takanobu Motoshima, Junji Yatsuda, Shigeyuki Esumi, Yuji Miura, Tomomi Kamba, Yoshihiro Komohara","doi":"10.1007/s13577-024-01110-w","DOIUrl":"10.1007/s13577-024-01110-w","url":null,"abstract":"CD74 is a transmembrane protein that functions as a specialized chaperone of HLA class II and CD74 in tumor cells was suggested to be involved in cell proliferation in several kinds of malignant tumors. CD74 is also known to be expressed in macrophages, therefore, we investigated the CD74 expression in clear cell renal cell carcinoma (ccRCC). Immunohistochemistry of CD74 indicated that CD74 was expressed not only in cancer cells but also macrophages. CD74 was detected in surface membrane and cytoplasm of cancer cells in 92 of 94 cases (98%) and of 87 of 94 cases (93%). CD74 was expressed both in cancer cells and TAMs in 86 of 94 cases (91%). In vitro studies using cancer cell lines and monocyte-derived macrophages stimulated by anti-CD74 antibodies showed that CD74 signal accelerated cancer cell proliferation and macrophage activation. However, macrophage activation via CD74 signal did not influence macrophage-mediated cancer cell growth. RNA-sequence of macrophages stimulated by anti-CD74 antibodies indicated that CD74 signal was associated to inflammatory responses in macrophages. In conclusion, we examined the expression and functional significance of CD74 in ccRCC using tissue specimens and cell culture studies. The function of CD74 was suggested to be different in cancer cells and in macrophages, and further studies are necessary to clarify the functional significance of CD74 in ccRCC.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141856899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTED ARTICLE: Wnt, notch signaling and exercise: what are their functions? Wnt、notch 信号和运动：它们的功能是什么？

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-02-22 DOI: 10.1007/s13577-024-01036-3

Yijie Zhao, Guangjun Wang, Zhifeng Wei, Duo Li, Mohammadamin Morshedi

引用次数: 0

The origin of ovarian Leydig cells: a possibly solved enigma? 卵巢Leydig细胞的起源：一个可能解开的谜团？

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-05 DOI: 10.1007/s13577-024-01098-3

José-Luis Carrasco-Juan, Miriam González-Gómez, Olga Tapia, Sonia García-Hernández, Abian Vega-Falcón, Rafael Méndez-Medina, Hugo Álvarez-Argüelles Cabrera, Lucio Díaz-Flores

引用次数: 0

Recent advances in nanomaterials for the treatment of femoral head necrosis. 纳米材料治疗股骨头坏死的最新进展。

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-12 DOI: 10.1007/s13577-024-01102-w

Yalin Yuan, Mi Zou, Shuqin Wu, Congcong Liu, Liang Hao

{"title":"Recent advances in nanomaterials for the treatment of femoral head necrosis.","authors":"Yalin Yuan, Mi Zou, Shuqin Wu, Congcong Liu, Liang Hao","doi":"10.1007/s13577-024-01102-w","DOIUrl":"10.1007/s13577-024-01102-w","url":null,"abstract":"Osteonecrosis of the femoral head (ONFH) is a condition that causes considerable pain and discomfort for patients, and its pathogenic mechanisms are not yet fully understood. While there have been many studies that suggest multiple factors may contribute to its development, current treatments involve both surgical and nonsurgical options. However, there is still much room for improvement in these treatment methods, particularly when it comes to preventing postoperative complications and optimizing surgical procedures. Nanomaterials, as a type of small molecule material, have shown great promise in treating bone tissue diseases, including ONFH. In fact, several nanocomposite materials have demonstrated specific effects in preventing ONFH, promoting bone tissue repair and growth, and optimizing surgical treatment. This article provides a comprehensive overview of current treatments for ONFH, including their advantages and limitations, and reviews the latest advances in nanomaterials for treating this condition. Additionally, this article explores the therapeutic mechanisms involved in using nanomaterials to treat ONFH and to identify new methods and ideas for improving outcomes for patients.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141591867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing the antitumor effects of metformin on ovarian clear cell carcinoma. 评估二甲双胍对卵巢透明细胞癌的抗肿瘤作用

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-08-08 DOI: 10.1007/s13577-024-01116-4

Satoshi Takemori, Tohru Morisada, Makoto Osaka, Momoe Watanabe, Atsushi Tajima, Shinji Tanigaki, Yoichi Kobayashi

{"title":"Assessing the antitumor effects of metformin on ovarian clear cell carcinoma.","authors":"Satoshi Takemori, Tohru Morisada, Makoto Osaka, Momoe Watanabe, Atsushi Tajima, Shinji Tanigaki, Yoichi Kobayashi","doi":"10.1007/s13577-024-01116-4","DOIUrl":"10.1007/s13577-024-01116-4","url":null,"abstract":"Developing novel therapies that outperform the existing chemotherapeutic treatments is required for treatment-resistant ovarian clear cell carcinoma. We investigated the antitumor effect of metformin on ovarian clear cell carcinoma, enhancement of the antitumor effect by its combination with chemotherapy, and its molecular regulatory mechanism. First, we evaluated the viability of ovarian clear cell carcinoma lines using the water-soluble tetrazolium-1 assay and found that metformin suppressed cell viability. Cell viability was significantly suppressed by co-treatment with cisplatin and metformin. In contrast, co-treatment with paclitaxel and metformin showed no significant difference in viability compared with the group without metformin. Western blot analysis showed increased phosphorylation of AMP-activated protein kinase in some cell lines and suppressed phosphorylation of the mammalian target of rapamycin in a particular cell line. Flow cytometry analysis revealed a significant increase in the rate of apoptosis in the metformin-treated group and rate of cell cycle arrest at the G2/M phase in a particular cell line. These results indicated that metformin may be effective against cultured ovarian clear cell carcinoma cells, particularly in combination with cisplatin.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141903393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re: Identification of a family with van der Hoeve's syndrome harboring a novel COL1A1 mutation and generation of patient-derived iPSC lines and CRISPR/Cas9-corrected isogenic iPSCs. 关于确定一个携带新型 COL1A1 基因突变的范德胡夫综合征家族，并生成源自患者的 iPSC 株系和经 CRISPR/Cas9 校正的同源 iPSC。

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-06-15 DOI: 10.1007/s13577-024-01093-8

Raymond Dalgleish

引用次数: 0

Pathophysiological role of Na-Cl cotransporter in kidneys, blood pressure, and metabolism. Na-Cl 共转运体在肾脏、血压和新陈代谢中的病理生理作用。

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-10 DOI: 10.1007/s13577-024-01099-2

Ran You, Zhanjun Jia

{"title":"Pathophysiological role of Na-Cl cotransporter in kidneys, blood pressure, and metabolism.","authors":"Ran You, Zhanjun Jia","doi":"10.1007/s13577-024-01099-2","DOIUrl":"10.1007/s13577-024-01099-2","url":null,"abstract":"The Na-Cl cotransporter (NCC) is a well-recognized regulator of ion transportation in the kidneys that facilitates Na+ reabsorption in the distal convoluted tubule. It is also the pharmacologic inhibitory target of thiazide diuretics, a class of front-line antihypertensive agents that have been widely used for decades. NCC is a potent regulator of Na+ reabsorption and homeostasis. Hence, its overactivation and suppression lead to hypertension and hypotension, respectively. Genetic mutations that affect NCC function contribute to several diseases such as Gordon and Gitelman syndromes. We summarized the role of NCC in various physiologic processes and pathological conditions, such as maintaining ion and water homeostasis, controlling blood pressure, and influencing renal physiology and injury. In addition, we discussed the recent advancements in understanding cryo-EM structure of NCC, the regulatory mechanisms and binding mode of thiazides with NCC, and novel physiologic implications of NCC in regulating the cross-talk between the immune system and adipose tissue or the kidneys. This review contributes to a comprehensive understanding of the pivotal role of NCC in maintaining ion homeostasis, regulating blood pressure, and facilitating kidney function and NCC's novel role in immune and metabolic regulation.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":null,"pages":null},"PeriodicalIF":3.4,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The function of long non-coding RNA IFNG-AS1 in autoimmune diseases. 长非编码 RNA IFNG-AS1 在自身免疫性疾病中的功能

IF 3.4 3区生物学

Human Cell Pub Date : 2024-09-01 Epub Date: 2024-07-14 DOI: 10.1007/s13577-024-01103-9

Jiale Zhao, Yibei Gui, Wei Wu, Xueqing Li, Lijun Wang, Hailin Wang, Yiyang Luo, Gang Zhou, Chengfu Yuan

引用次数: 0