患者源性舌鳞状细胞癌细胞系的建立与鉴定。

IF 3.4 3区 生物学 Q3 CELL BIOLOGY
Priyanka Joshi, Sanjay Bane, Pankaj Chaturvedi, Poonam Gera, Sanjeev K Waghmare
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引用次数: 0

摘要

口腔鳞状细胞癌(OSCC)是一种常见的癌症在印度人口,其中三分之一的全球OSCC病例来自印度。由于诊断较晚,5年生存率较低。口腔舌鳞状细胞癌(OTSCC)是第二常见的OSCC。体外细胞系模型是深入了解治疗耐药和疾病进展的分子机制的有价值的工具。我们报告了从晚期治疗naïve印度患者样本中建立的三个OTSCC细胞系,如ACOTSC120, ACOSTC132和ACOTSC140。3株OTSCC细胞系均呈上皮形态,经角蛋白-14染色证实。这些细胞系显示出体外球状形成和体内致瘤潜能。与DSMZ细胞系数据库相比,细胞系的STR确保了其人类来源和新颖性。细胞系的核型显示为非整倍体,进一步证实了它们的人类起源。这些细胞系显示存在癌症干细胞(CSCs)群体,即ALDHbr/CD44+群体。因此,这些细胞系提供了一个模型,以帮助了解疾病的生物学及其进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines.

Oral squamous cell carcinoma (OSCC) is a common carcinoma in Indian population, wherein one-third of global OSCC cases are from India. The five-year survival rate is poor due to late diagnosis. Oral tongue squamous cell carcinoma (OTSCC) is the second-most common OSCC. An in vitro cell line model is a valuable tool to get a deeper understanding of the molecular mechanisms involved in therapy resistance and disease progression. We report establishment of three OTSCC cell lines from advanced stage treatment naïve Indian patient samples, such as ACOTSC120, ACOSTC132, and ACOTSC140. All three OTSCC cell lines showed epithelial morphology, which was confirmed by Keratin-14 staining. The cell lines showed in vitro spheroid-forming and in vivo tumorigenic potential. The STR of the cell lines ensured their human origin and novelty when compared to DSMZ cell line database. The karyotype of the cell lines showed aneuploidy and further confirmed their human origin. These cell lines showed the presence of cancer stem cell (CSCs) population, i.e., the ALDHbr/CD44+ population. These cell lines thus provide a model to help understand the biology of disease and its progression.

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来源期刊
Human Cell
Human Cell CELL BIOLOGY-
CiteScore
5.90
自引率
2.30%
发文量
176
审稿时长
4.5 months
期刊介绍: Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.
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