IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-28 DOI: 10.1007/s13577-025-01243-6

Zekun Li, Luyun Liu, Yuhui Sun, Xinjuan Liu, Ping Zhang, Yue Wang, Gang Ding

{"title":"Mesenchymal stem/stromal cells-derived exosomes: possible therapeutic mechanism in inflammatory bowel disease.","authors":"Zekun Li, Luyun Liu, Yuhui Sun, Xinjuan Liu, Ping Zhang, Yue Wang, Gang Ding","doi":"10.1007/s13577-025-01243-6","DOIUrl":"https://doi.org/10.1007/s13577-025-01243-6","url":null,"abstract":"Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract caused by dysfunction of the immune system in genetically susceptible individuals. As current pharmacologic and surgical treatments remain suboptimal, increasing attention has been directed toward exosomes derived from mesenchymal stem/stromal cells (MSCs) as alternative therapeutic approaches. MSCs are multipotent stromal cells that can be isolated from various human tissues such as bone marrow, adipose, umbilical cord and periodontal ligament. Exosomes are cell-derived membrane-bound vesicles enclosing RNAs, proteins, growth factors, and cytokines. Previous studies indicate that the anti-inflammatory, immunomodulatory, and regenerative effects of MSCs are largely mediated by MSC-derived exosomes (MSC-Exos). Therefore, this review outlines current insights into the molecular mechanisms of MSC-Exos in IBD treatment to support the future development of MSC-Exos as a therapeutic strategy, thus providing novel observations into the clinical applications of MSC-Exos in IBD management.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"111"},"PeriodicalIF":3.4,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell dynamic RNA and glycosylation sequencing reveals the mechanism underlying the differentiation of pluripotent stem cells into hematopoietic stem cells. 单细胞动态RNA和糖基化测序揭示了多能干细胞向造血干细胞分化的机制。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-27 DOI: 10.1007/s13577-025-01234-7

Wanyi Feng, Sheng Zeng, Donghui Liu, Wei Gong, Junjie Hu, Weihua Xu, Zhichao Ma, Shengmiao Fu, Xinping Chen

{"title":"Single-cell dynamic RNA and glycosylation sequencing reveals the mechanism underlying the differentiation of pluripotent stem cells into hematopoietic stem cells.","authors":"Wanyi Feng, Sheng Zeng, Donghui Liu, Wei Gong, Junjie Hu, Weihua Xu, Zhichao Ma, Shengmiao Fu, Xinping Chen","doi":"10.1007/s13577-025-01234-7","DOIUrl":"https://doi.org/10.1007/s13577-025-01234-7","url":null,"abstract":"Studying the mechanism of hematopoietic stem cells' generation from induced pluripotent stem cells in vitro can be useful for understanding embryonic hematopoiesis, as well as for the application of related cell therapy. This study aimed to delineate the process of the differentiation of induced pluripotent stem cells into hematopoietic stem cells' models and provide a theoretical basis and clinical value for the production of hematopoietic stem cells in vitro. We analyzed the differentiation model by single-cell dynamic transcriptome and glycosylation sequencing, which was divided into three differentiation stages based on the new-to-total RNA ratio and glycosylation level. Two differentiation fates were found in the pseudo-time, including hematopoietic development and other tissue development. Precursor hematopoietic cells with a high glycosylation level greatly expressed hematopoietic regulation and vascular endothelial genes, suggesting that glycosylation is associated with angiogenesis and hematopoietic regulation. The multiple differentiation events in the in vitro model are similar to those in hematopoietic development in vivo, including yolk sac hematopoiesis, cellular communication between non-potential hematopoietic subsets and potential hematopoietic subsets, gene expression, and temporal deviations in hematopoietic fate. Our study has revealed the similar hematopoiesis process in the differentiation model via single-cell dynamic RNA and glycosylation sequencing, which provides an important theoretical basis for the study of hematopoietic stem cell development.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"110"},"PeriodicalIF":3.4,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144162961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of NCC-MLPS4-C1: a novel patient-derived cell line of myxoid liposarcoma. NCC-MLPS4-C1：一种新的患者源性黏液样脂肪肉瘤细胞系的建立和表征

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-26 DOI: 10.1007/s13577-025-01241-8

Julia Osaki, Rei Noguchi, Takuya Ono, Yuki Adachi, Shuhei Iwata, Yomogi Shiota, Hiroya Kondo, Koichi Ogura, Shogo Nishino, Akihiko Yoshida, Akira Kawai, Tadashi Kondo

{"title":"Establishment and characterization of NCC-MLPS4-C1: a novel patient-derived cell line of myxoid liposarcoma.","authors":"Julia Osaki, Rei Noguchi, Takuya Ono, Yuki Adachi, Shuhei Iwata, Yomogi Shiota, Hiroya Kondo, Koichi Ogura, Shogo Nishino, Akihiko Yoshida, Akira Kawai, Tadashi Kondo","doi":"10.1007/s13577-025-01241-8","DOIUrl":"https://doi.org/10.1007/s13577-025-01241-8","url":null,"abstract":"Myxoid liposarcoma (MLPS) is a malignant tumor composed of uniform, round-to-ovoid cells and small lipoblasts embedded in a myxoid stroma containing branching capillaries. MLPS is characterized by the presence of fusion genes, primarily FUS::DDIT3, with EWSR1::DDIT3 identified in a subset of cases. Although multimodal therapies have improved outcomes for localized MLPS, the prognosis for recurrent or metastatic MLPS cases remains poor, underscoring the need for novel therapeutic strategies. Patient-derived cell lines are essential tools in cancer research, providing various opportunities for discovery to the researchers. However, publicly available MLPS cell lines are lacking, which hinders research on this disease. With this notion, we established an MLPS cell line, NCC-MLPS4-C1. NCC-MLPS4-C1 was derived from the surgically resected tumor of a 48-year-old male patient with MLPS. We performed molecular and phenotypic characterization and high-throughput drug screening to evaluate its potential as a preclinical model. Genetic analysis confirmed the presence of the FUS::DDIT3 fusion gene in the original tumor and NCC-MLPS4-C1. NCC-MLPS4-C1 demonstrated stable proliferation, in vitro spheroid-forming ability, and invasive characteristics. We demonstrated that NCC-MLPS4-C1 is applicable for the global experiments such as drug screening. In conclusion, we successfully established NCC-MLPS4-C1, a novel cell line derived from surgically resected tumor tissue. NCC-MLPS4-C1 will be useful for the cancer research, especially in which the cell lines are required for global experiments.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"109"},"PeriodicalIF":3.4,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced-stage Indian patient. 印度晚期患者新型皮肤鳞状细胞癌细胞系的建立及分子特征分析。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-25 DOI: 10.1007/s13577-025-01237-4

Darshan Mehta, Akshay Paradkar, Prakash Nayak, Bharat Rekhi, Bhabani Mohanty, Pradip Chaudhari, Sanjeev K Waghmare

{"title":"Establishment and molecular characterization of the novel cutaneous squamous cell carcinoma cell line from advanced-stage Indian patient.","authors":"Darshan Mehta, Akshay Paradkar, Prakash Nayak, Bharat Rekhi, Bhabani Mohanty, Pradip Chaudhari, Sanjeev K Waghmare","doi":"10.1007/s13577-025-01237-4","DOIUrl":"10.1007/s13577-025-01237-4","url":null,"abstract":"Cutaneous squamous cell carcinoma (CSCC) is the second most prevalent skin cancer with low metastatic potential; it poses significant morbidity challenges. CSCC possesses significant heterogeneity and the treatment presents a formidable challenge. To gain a clear insight into the diverse nature of these tumors, the development of an in vitro cell line model is essential. However, there are few cell lines that were established, and only one skin SCC cell line is available on the ATCC. In the present study, we established and characterized a novel ACSCC1 cell line from the advanced-stage treatment naïve cutaneous SCC originating from the forearm of the Indian patient. The keratin expression profile showed the epithelial origin of the cell line, ploidy and karyotyping revealed the hyperdiploid population; ACSCC1 showed an increased tumorigenic and metastatic potential. Further, our cell line showed higher invasive, migratory potential and epithelial-mesenchymal-transition (EMT). Additionally, the Transmission electron microscopy (TEM) results showed an aberrant mitochondrial morphology and reduction in the cellular junctions. Further, our whole genome sequencing (WGS) analysis showed mutations in the cancer-related genes. Overall, the novel ACSCC1 cell line can be used to decipher the molecular signaling in the cancer stem cells (CSCs); targeting the CSCs population may help in understanding the tumor recurrence.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"108"},"PeriodicalIF":3.4,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144144164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KDELR3 is transcriptionally activated by FOXM1 and accelerates lung adenocarcinoma growth and metastasis via inhibiting endoplasmic reticulum stress-induced cell apoptosis. KDELR3被FOXM1转录激活，并通过抑制内质网应激诱导的细胞凋亡来加速肺腺癌的生长和转移。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-24 DOI: 10.1007/s13577-025-01238-3

Cheng Wang, Zhaoxuan Wang, Shiqing Wang, Lin Jing, Chundong Gu

{"title":"KDELR3 is transcriptionally activated by FOXM1 and accelerates lung adenocarcinoma growth and metastasis via inhibiting endoplasmic reticulum stress-induced cell apoptosis.","authors":"Cheng Wang, Zhaoxuan Wang, Shiqing Wang, Lin Jing, Chundong Gu","doi":"10.1007/s13577-025-01238-3","DOIUrl":"https://doi.org/10.1007/s13577-025-01238-3","url":null,"abstract":"Lung cancer is still considered to be the leading cause of cancer-related death worldwide, and lung adenocarcinoma (LUAD) is the most common kind. KDEL Endoplasmic Reticulum Protein Retention Receptor 3 (KDELR3) is a critical regulator of the endoplasmic reticulum (ER) stress and the followed unfolded protein response (UPR) process, which are critical in tumor development. However, the role of KDELR3 in LUAD tumor progression remains poorly understood. In this work, we demonstrated that KDELR3 is significantly upregulated in LUAD tumor tissues and cell lines. Suppression of KDELR3 promoted the phosphorylation level of UPR-related pathways, PERK, and EIF2α in LUAD cell lines. The downregulation of KDELR3 promoted ER stress-induced cell apoptosis, decreased the protein expression of Bcl-2, and increased the protein expression of Bax in LUAD cells. Moreover, the knockdown of KDELR3 inhibits LUAD cell invasion. In vivo animal experiments confirmed that the inhibition of KDELR3 suppresses LUAD tumor growth and metastasis. Mechanistic studies showed that transcription factor FOXM1 may serve as an upstream factor of KDELR3. The upregulation of FOXM1 increased the transcriptional activity of KDELR3. Further results illustrated that FOXM1 directly binds to the promoter of KDELR3, thus upregulating its expression. Finally, rescue experiments demonstrated that FOXM1 inhibition-induced cell apoptosis and invasion could be reversed by KDELR3 overexpression. Overall, our findings indicated that KDELR3 is transcriptionally upregulated by FOXM1 and accelerates tumor growth and lung metastasis in LUAD by inhibiting ER stress-induced cell apoptosis.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"106"},"PeriodicalIF":3.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The glycocalyx: a key target for treatment of severe acute pancreatitis-associated multiple organ dysfunction syndrome. 糖萼：治疗重症急性胰腺炎相关多器官功能障碍综合征的关键靶点。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-24 DOI: 10.1007/s13577-025-01227-6

Huijuan Li, Haiyun Wen, Jie Liu, Xinyu Luo, Boliang Pei, Peng Ge, Zhenxuan Sun, Jin Liu, Junjie Wang, Hailong Chen

{"title":"The glycocalyx: a key target for treatment of severe acute pancreatitis-associated multiple organ dysfunction syndrome.","authors":"Huijuan Li, Haiyun Wen, Jie Liu, Xinyu Luo, Boliang Pei, Peng Ge, Zhenxuan Sun, Jin Liu, Junjie Wang, Hailong Chen","doi":"10.1007/s13577-025-01227-6","DOIUrl":"10.1007/s13577-025-01227-6","url":null,"abstract":"The endothelial glycocalyx is a dynamic brush-like layer composed of proteoglycans and glycosaminoglycans, including heparan sulfate (HS) and hyaluronic acid (HA), and is an important regulator of vascular homeostasis. Its structure (thickness ranges from 20 to 6450 nm in different species) not only provides a charge-selective barrier but also serves to anchor mechanosensors such as the glypican-1 (GPC-1)/caveolin-1 (CAV-1) complex and buffers shear stress. In severe acute pancreatitis (SAP), inflammatory factors promote the expression of matrix metalloproteinases (MMPs) and heparinases, which degrade syndecan-1 (SDC-1) and HS, while oxidative stress disrupts HA-CD44 binding, leading to increased capillary leakage and neutrophil adhesion. This degradation process occurs before the onset of multiple organ dysfunction syndrome (MODS), highlighting the potential of the glycocalyx as an early biomarker. More importantly, the regeneration of glycocalyx through endothelial cell synthesis of glycosaminoglycans (GAGs) and shear stress-driven SDC recycling provides therapeutic prospects. This review redefines the pathophysiology of severe acute pancreatitis-associated multiple organ dysfunction (SAP-MODS) by exploring the glycocalyx's central mechanistic role and proposes stabilizing glycocalyx structure as a potential strategy to prevent microcirculatory failure.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"107"},"PeriodicalIF":3.4,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ITGA5 drives angiogenesis in diabetic retinopathy via TAK-1/NF-kB activation. ITGA5通过TAK-1/NF-kB激活驱动糖尿病视网膜病变血管生成。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-23 DOI: 10.1007/s13577-025-01233-8

Feifei Kan, Di Wang, Sijia Li, Yi Gao, Jianwen Wang

{"title":"ITGA5 drives angiogenesis in diabetic retinopathy via TAK-1/NF-kB activation.","authors":"Feifei Kan, Di Wang, Sijia Li, Yi Gao, Jianwen Wang","doi":"10.1007/s13577-025-01233-8","DOIUrl":"https://doi.org/10.1007/s13577-025-01233-8","url":null,"abstract":"Diabetic retinopathy is a retinal damage, which causes vision impairment and blindness. Integrin Subunit Alpha 5 (ITGA5) regulates angiogenic response, but its roles in diabetic retinopathy remain unclear. In this work, diabetes mellitus was induced in rats by streptozotocin. ITGA5 interference was achieved by intravitreal delivery of adeno-associated virus. Upregulation of ITGA5 was found in diabetic rat retinal tissues. ITGA5 knockdown decreased the neovascularization, acellular capillary formation, and pericytes. The protein expression of vascular endothelial growth factor (VEGFA), vascular adhesion molecule-1(VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) was reduced after ITGA5 interference. Besides, ITGA5 knockdown decreased the phosphorylation level of FAK, TAK-1, and p65. In vitro, rat retinal microvascular endothelial cells (RRMECs) were cultured under high glucose condition to stimulate diabetic environment. ITGA5 knockdown inhibited VEGFA secretion, tube formation, cell invasion, and migration. Upregulation of VCAM-1 and ICAM-1 that induced by high glucose was reversed by ITGA5 silencing. ITGA5 knockdown blocked the activation of TAK-1/NF-kB pathway in RRMECs. Additionally, in oxygen-induced retinopathy model, ITGA5 interference inhibited pathological neovascularization. These results demonstrate that ITGA5 contributes to the angiogenesis in diabetic retinopathy by activating TAK-1/NF-kB pathway.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"105"},"PeriodicalIF":3.4,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144132653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secretory pathway in honeybee venom and actin: evidence from chemical-biological interactions. 蜂毒和肌动蛋白的分泌途径：来自化学-生物相互作用的证据。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-23 DOI: 10.1007/s13577-025-01240-9

Amr Ahmed El-Arabey

引用次数: 0

Potential reverse functions of GOLPH3 and GOLPH3L in pyroptotic cell death, with implications in resistance to radiotherapy and chemotherapy. GOLPH3和GOLPH3L在热腐细胞死亡中的潜在逆转功能，与放疗和化疗耐药性有关。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-22 DOI: 10.1007/s13577-025-01236-5

Caglar Berkel

引用次数: 0

Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines. 患者源性舌鳞状细胞癌细胞系的建立与鉴定。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-05-20 DOI: 10.1007/s13577-025-01231-w

Priyanka Joshi, Sanjay Bane, Pankaj Chaturvedi, Poonam Gera, Sanjeev K Waghmare

{"title":"Establishment and characterization of patient-derived tongue squamous cell carcinoma cell lines.","authors":"Priyanka Joshi, Sanjay Bane, Pankaj Chaturvedi, Poonam Gera, Sanjeev K Waghmare","doi":"10.1007/s13577-025-01231-w","DOIUrl":"10.1007/s13577-025-01231-w","url":null,"abstract":"Oral squamous cell carcinoma (OSCC) is a common carcinoma in Indian population, wherein one-third of global OSCC cases are from India. The five-year survival rate is poor due to late diagnosis. Oral tongue squamous cell carcinoma (OTSCC) is the second-most common OSCC. An in vitro cell line model is a valuable tool to get a deeper understanding of the molecular mechanisms involved in therapy resistance and disease progression. We report establishment of three OTSCC cell lines from advanced stage treatment naïve Indian patient samples, such as ACOTSC120, ACOSTC132, and ACOTSC140. All three OTSCC cell lines showed epithelial morphology, which was confirmed by Keratin-14 staining. The cell lines showed in vitro spheroid-forming and in vivo tumorigenic potential. The STR of the cell lines ensured their human origin and novelty when compared to DSMZ cell line database. The karyotype of the cell lines showed aneuploidy and further confirmed their human origin. These cell lines showed the presence of cancer stem cell (CSCs) population, i.e., the ALDHbr/CD44+ population. These cell lines thus provide a model to help understand the biology of disease and its progression.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"102"},"PeriodicalIF":3.4,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12092557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144112561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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