IF 3.4 3区生物学

Human Cell Pub Date : 2025-07-09 DOI: 10.1007/s13577-025-01255-2

Pooja Tiwary, Krishil Oswal, Ryan Varghese, Harsh Anchan, Mitul Oswal

{"title":"Multi-epitope vaccines: charting a new frontier in monkeypox prevention and control.","authors":"Pooja Tiwary, Krishil Oswal, Ryan Varghese, Harsh Anchan, Mitul Oswal","doi":"10.1007/s13577-025-01255-2","DOIUrl":"https://doi.org/10.1007/s13577-025-01255-2","url":null,"abstract":"Monkeypox is a viral zoonotic infection that has emerged as a significant public health threat recently. The world has experienced a global outbreak of the Monkeypox virus (MPXV), with 124,753 confirmed cases in 128 countries and a total of 272 fatalities as of February 13, 2025. This alarming increase in cases demands immediate attention to the underlying causes of the surge. The World Health Organization (WHO) has endorsed the use of the MVA-BN vaccine, which was previously approved for smallpox prevention. However, there are currently no antiviral treatments approved by the FDA for MPXV. In addition, the emergence of mutations in MPXV strains poses challenges for the development and effectiveness of viable vaccines. Developing conventional vaccines is typically expensive, time-consuming, and requires extensive validation processes. Moreover, these vaccines often demonstrate suboptimal efficacy against emerging viral strains. As a response to these challenges, multi-epitope vaccines have gained significant interest for their potential to activate B-cell and T-cell responses, providing prolonged immunological activity against pathogens. These vaccines feature a targeted approach, offering chemical stability, non-infectious properties, rapid and cost-effective production, enhanced safety, and the potential to elicit a robust immune response. Several studies employing immunoinformatics have confirmed the efficacy of multi-epitope vaccines, designed to provide comprehensive immune protection against MPXV. These vaccines promise to deliver a strong immune response, better coverage against various viral strains and variants, and improved stability and effectiveness. In addition, they are expected to be non-allergenic, non-toxic, and highly antigenic. While promising results have been reported regarding the use of these vaccines for monkeypox, further research on their efficacy, delivery systems, and additional preclinical and clinical trials is crucial to maximize their benefits for humanity.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 5","pages":"126"},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144592789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of preclinical model of primary ovarian squamous cell carcinoma. 原发性卵巢鳞状细胞癌临床前模型的建立与表征。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-07-07 DOI: 10.1007/s13577-025-01253-4

Chenyang Xu, Weiming Xu, Conghui Wu, Chen Xu, Zhengxiao Ma, Jiamin Wang, Yue Zhuo, Xinan Cai, Yuting Zhang, Yingqi Lyu, Jinchao Wang, Minran Huang, Shu Sun, Tingting Feng, Lisha Ying, Dan Su

{"title":"Establishment and characterization of preclinical model of primary ovarian squamous cell carcinoma.","authors":"Chenyang Xu, Weiming Xu, Conghui Wu, Chen Xu, Zhengxiao Ma, Jiamin Wang, Yue Zhuo, Xinan Cai, Yuting Zhang, Yingqi Lyu, Jinchao Wang, Minran Huang, Shu Sun, Tingting Feng, Lisha Ying, Dan Su","doi":"10.1007/s13577-025-01253-4","DOIUrl":"https://doi.org/10.1007/s13577-025-01253-4","url":null,"abstract":"Primary ovarian squamous cell carcinoma (POSCC) represents an exceedingly rare subtype of epithelial ovarian cancer (EOC) characterized by a cryptic etiology and insidious onset. The rarity and high mortality associated with pure primary ovarian squamous cell carcinoma (SCC) make it challenging to conduct large randomized controlled studies. Furthermore, there are currently no commercially available ovarian SCC cell lines for research purposes, necessitating the urgent establishment of novel lines. To our knowledge, this study reports the first preclinical model of primary ovarian squamous cell carcinoma (POSCC), denoted as ZOC254. We have detailed the establishment and characterization of ZOC254, derived from a 64-year-old female patient, which preserves the primary tumor's original traits across various levels during prolonged in vitro expansion. Whole-exome sequencing (WES) of both the primary tumor and derived cell line revealed homologous recombination deficiency (HRD) and high tumor mutational burden (TMB). ZOC254 also exhibited the PIK3CA: p.E542K mutation associated with targeted therapy. The effectiveness of olaparib, everolimus, and conventional chemotherapeutic agents for ovarian cancer was preliminarily assessed on the growth of patient-derived cells (PDC). The POSCC cell line and derived xenograft transplantation model reported in this study serve the purpose of broadening the resources accessible for preclinical investigations into ovarian squamous carcinoma.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 5","pages":"125"},"PeriodicalIF":3.4,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144585469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosomes derived from human umbilical cord mesenchymal stem cells inhibit hepatocyte pyroptosis via miR-423-5p/ZBP1 in acute liver failure. 来自人脐带间充质干细胞的外泌体在急性肝衰竭中通过miR-423-5p/ZBP1抑制肝细胞焦亡。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-07-04 DOI: 10.1007/s13577-025-01248-1

Dan Xie, Lina Yu, Ziyang Wang, Gongqin Qiu, Shi Ouyang

{"title":"Exosomes derived from human umbilical cord mesenchymal stem cells inhibit hepatocyte pyroptosis via miR-423-5p/ZBP1 in acute liver failure.","authors":"Dan Xie, Lina Yu, Ziyang Wang, Gongqin Qiu, Shi Ouyang","doi":"10.1007/s13577-025-01248-1","DOIUrl":"10.1007/s13577-025-01248-1","url":null,"abstract":"Human umbilical cord mesenchymal stromal cells (hucMSCs) have emerged as a promising therapeutic option for acute liver failure (ALF). However, the detailed mechanism by which hucMSCs modulate hepatocyte pyroptosis in ALF remains unclear. In this study, we induced ALF in mice using acetaminophen (APAP). Mice were intravenously injected with 1 × 106 hucMSCs/ hucMSCs-Exo via the tail vein 6 h after APAP administration. Liver pathological changes were assessed by hematoxylin and eosin (H&E) staining. Subsequently, an in vitro model of liver cell failure and pyroptosis model was established using LO2 cells treated with lipopolysaccharide (LPS) and adenosine 5'-triphosphate (ATP). The levels of cell pyroptosis marker caspase-1 were detected by flow cytometry analysis, RT-qPCR assay, and western blot assay. The study employed a comprehensive approach, including flow cytometry analysis, RT-qPCR assay, miRNA sequencing, and luciferase reporter gene experiments. hucMSCs and hucMSCs- exosomes (MSCs-Exo) inhibited cell inflammation to improve ALF in vivo model of ALF and inhibited hepatocyte pyroptosis in LO2 cells induced by ATP and LPS. MiR-423-5p emerged as a potential mediator of the anti-pyroptotic effects of hucMSCs-Exo, with ZBP1 identified as one of its downstream targets. Subsequent validation confirmed that miR-423-5p targets ZBP1 to regulate pyroptosis. These findings highlight the role of hucMSCs-derived exosomal miR-423-5p in inhibiting hepatocyte pyroptosis in LO2 cells induced by ATP and LPS. miR-423-5p serves as a crucial mediator in this process, targeting ZBP1, a protein significantly involved in the pyroptotic pathway. Our findings may provide basics for further research on mechanism of hucMSCs and present a promising cell-free strategy treating ALF.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 5","pages":"124"},"PeriodicalIF":3.4,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144561649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CircTADA2A inhibits cell proliferation and promotes ferroptosis by sponging miR-638 in acute myeloid leukemia. 在急性髓系白血病中，CircTADA2A通过海绵化miR-638抑制细胞增殖并促进铁凋亡。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-07-03 DOI: 10.1007/s13577-025-01251-6

Yuan Yuan, Jiajia Li, Meng Wang, Yan Jin, Ruixiang Xia

{"title":"CircTADA2A inhibits cell proliferation and promotes ferroptosis by sponging miR-638 in acute myeloid leukemia.","authors":"Yuan Yuan, Jiajia Li, Meng Wang, Yan Jin, Ruixiang Xia","doi":"10.1007/s13577-025-01251-6","DOIUrl":"https://doi.org/10.1007/s13577-025-01251-6","url":null,"abstract":"Dysregulation of circRNAs has been found to engage in the progression of many hematologic malignancies including acute myeloid leukemia (AML). In this study, we identified significantly downregulated circTADA2A, derived from linear transcriptional adaptor 2A (TADA2A) gene, in AML associated circRNAs microarrays using GEO2R tool. We aimed to elucidate the roles of circTADA2A in AML and the mechanisms involved. Quantitative reverse transcription PCR was used for verification of circTADA2A levels in AML specimens, and its diagnostic value and clinical significance were assessed. The effects of circTADA2A on proliferation and ferroptosis on THP-1 and HL-60 cells were carried out using cell counting kit-8, 5'-ethynyl-2'-deoxyuridine, Fe2+, lipid reactive oxygen species (ROS) and malondialdehyde (MDA) assays. Luciferase reporter, fluorescence in situ hybridization, RNA immunoprecipitation, and RNA pull-down assays were implemented to investigate the potential miRNAs that mediated circTADA2A functioning. We confirmed that circTADA2A levels were lowly expressed in plasma and bone marrow of AML patients, and associated with bone marrow blasts and cytogenetic risk. Plasma circTADA2A had a high sensitivity and specificity with an area under the curve value of 0.793 in differentiating AML patients from healthy individuals. THP-1 and HL-60 cells stably overexpressing circTADA2A exhibited reduced cell proliferation, and sensitized cell to ferroptosis by a ferroptosis inducer RSL3. Moreover, circTADA2A could counteracted Ferrostatin-1-induced inhibition of ferroptosis. Mechanistically, circTADA2A act as a sponge for miR-638, and upregulation of miR-638 expression could restore cellular phenotypes induced by circTADA2A. Our findings demonstrated that circTADA2A suppresses cell proliferation and promotes ferroptosis by sponging miR-638 during AML progression.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 5","pages":"123"},"PeriodicalIF":3.4,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144555520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and characterization of NCC-EMC1-C1: a novel patient-derived cell line of extraskeletal myxoid chondrosarcoma. NCC-EMC1-C1的建立和表征：一种新的患者来源的骨外粘液样软骨肉瘤细胞系。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-06-28 DOI: 10.1007/s13577-025-01250-7

Shuhei Iwata, Rei Noguchi, Julia Osaki, Yuki Adachi, Yomogi Shiota, Shuhei Osaki, Shogo Nishino, Akihiko Yoshida, Seiji Ohtori, Akira Kawai, Tadashi Kondo

{"title":"Establishment and characterization of NCC-EMC1-C1: a novel patient-derived cell line of extraskeletal myxoid chondrosarcoma.","authors":"Shuhei Iwata, Rei Noguchi, Julia Osaki, Yuki Adachi, Yomogi Shiota, Shuhei Osaki, Shogo Nishino, Akihiko Yoshida, Seiji Ohtori, Akira Kawai, Tadashi Kondo","doi":"10.1007/s13577-025-01250-7","DOIUrl":"10.1007/s13577-025-01250-7","url":null,"abstract":"Extraskeletal myxoid chondrosarcoma (EMC) is a rare soft tissue sarcoma characterized by a myxoid matrix and a distinctive lobulated architecture, composed of cords and clusters of uniform round-to-rhabdoid cells. At the molecular level, EMC is defined by specific gene fusions involving NR4A3, most frequently EWSR1::NR4A3. The responses to conventional chemotherapy are limited, and the prognosis for patients with advanced or metastatic disease remains poor. We successfully developed the NCC-EMC1-C1 cell line using surgically resected tumor tissue from a patient with EMC. NCC-EMC1-C1 cells exhibited constant proliferation in monolayer culture, spheroid formation in low-attachment plates, and migration. High-throughput screening of 221 anticancer drugs using NCC-EMC1-C1 identified three candidates, brigatinib, panobinostat, and romidepsin, that demonstrated low IC50 values. These data indicated the utility of NCC-EMC1-C1 for the experiments based on screening. We conclude that NCC-EMC1-C1 is a valuable tool for preclinical and basic research on EMC.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"122"},"PeriodicalIF":3.4,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144530701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of human-induced pluripotent stem cells from a patient with Beckwith-Wiedemann syndrome. 贝克威氏综合征患者的人诱导多能干细胞的生成。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-06-27 DOI: 10.1007/s13577-025-01247-2

Mei Tang, Jiafan Lei, Congwen Shao, Wenyan Zhou, Min Xiong, Min Huang, Chunyan Huang, Fei Wang, Jun Liu, Jun Li, Xueqing Xu

引用次数: 0

Identification of autophagy-related biomarkers in prostate cancer prognosis. 前列腺癌预后中自噬相关生物标志物的鉴定。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-06-23 DOI: 10.1007/s13577-025-01249-0

Yancheng Di, Linlin Zhao, Lingling Zhang, Lei Chen

{"title":"Identification of autophagy-related biomarkers in prostate cancer prognosis.","authors":"Yancheng Di, Linlin Zhao, Lingling Zhang, Lei Chen","doi":"10.1007/s13577-025-01249-0","DOIUrl":"10.1007/s13577-025-01249-0","url":null,"abstract":"Prostate cancer is the second leading cause of cancer-related deaths in males that has an unfavorable outcome. Autophagy-related genes (ARGs) contribute to the process of tumorigenesis and metastasis of prostate cancer. This study aimed to identify ARGs that could serve as reliable and non-invasive biomarkers for evaluating prostate cancer prognosis. The expression profiles of ARGs were identified in prostate cancer specimens with good prognosis (n = 98) and poor prognosis (n = 42). A series of in vitro assays were performed to explore the function and mechanisms of ARGs in malignant progression of prostate cancer. Receiver operating characteristic curve were utilized to evaluate the predictive potential of ARGs for prostate cancer prognosis. Patients with poor prognosis exhibited higher expression of baculoviral inhibitor of apoptosis repeat containing 5 (BIRC5) and lower expression of neuregulin 2 (NRG2) compared to those with good prognosis. BIRC5 served as independent risk factors for prostate cancer prognosis, and enhanced BIRC5 expression promoted cells viability, migration, and invasion, but the autophagy activator rapamycin could counteract the effects of the BIRC5 gene. Conversely, NRG2 acted as a protective factor for prostate cancer prognosis, and elevated NRG2 expression suppressed cells viability, migration, and invasion, but the autophagy inhibitor 3-Methyladenine could reverse the effects of the NRG2 gene. The combination of BIRC5, NRG2 with prostate specific antigen (PSA) demonstrated significant predictive value for prostate cancer prognosis. BIRC5 and NRG2 genes participate in the progression of prostate cancer by regulating autophagy. BIRC5 and NRG2 have the potential to serve as valuable biomarkers for the prognosis of prostate cancer.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"120"},"PeriodicalIF":3.4,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144477480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EZH2 regulates tumor-associated macrophages by the KDM6A-mediated inflammatory response in HPV16-positive cervical cancer. EZH2在hpv16阳性宫颈癌中通过kdm6a介导的炎症反应调节肿瘤相关巨噬细胞。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-06-19 DOI: 10.1007/s13577-025-01246-3

Qing Tang, Ying Yang, Jia'nan Sun

{"title":"EZH2 regulates tumor-associated macrophages by the KDM6A-mediated inflammatory response in HPV16-positive cervical cancer.","authors":"Qing Tang, Ying Yang, Jia'nan Sun","doi":"10.1007/s13577-025-01246-3","DOIUrl":"10.1007/s13577-025-01246-3","url":null,"abstract":"High-risk HPV subtypes impact the immune response in cervical cancer. Tumor-associated macrophages (TAMs) are well known to contribute to tumor development by regulating the immune response. This study aimed to analyze the mechanism of TAMs by the enhancer of zeste homolog 2 (EZH2) in HPV16+ cervical cancer. The HPV16+ cervical cancer cells, SiHa and CaSki, were treated with increasing concentrations of EZH2 inhibitor EPZ6438 (5, 10, 20, 40, 80 μM) for 24 h. The KDM6A expression is suppressed in a concentration-dependent manner when EZH2 activity is inhibited. Then, the cancer cells were transfected with pcDNA-control or pcDNA-KDM6A, and treated with the IC50 of EPZ6438. The cell viability, levels of IL-6 and CXCL1, and p-Akt(s473)/Akt proteins were measured. The PMA-treated THP-1 cells were induced into M2 macrophages by IL-4 and IL-13. The M2 macrophages were cocultured with the conditioned cancer cells to observe the M2 polarization. In vivo experiments, the effects of EZH2 inhibition on tumor growth were investigated in nude mice. EZH2 inhibition suppressed the cell viability and inflammatory response by suppressing KDM6A in HPV16+ cervical cancer cells. KDM6A overexpression suppressed the effects of EZH2 inhibition on cell viability and inflammatory response. EZH2 inhibition in cancer cells suppressed the M2 macrophages, and its mechanism was related to the KDM6A-mediated inflammatory response. In nude mice models, EZH2 inhibition effectively reduced tumor growth by regulating KDM6A. EZH2 regulated the KDM6A-mediated inflammatory response, thus affecting the polarization of M2 macrophages, leading to tumor growth in HPV16+ cervical cancer. This study provided an insight into the immune modulation of EZH2 in HPV16+ cervical cancer.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"119"},"PeriodicalIF":3.4,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144327497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human metapneumovirus: a mini-review on the genetic and phylogenetic roots of a rising respiratory threat. 人偏肺病毒：一种不断上升的呼吸道威胁的遗传和系统发育根源的综述。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-06-11 DOI: 10.1007/s13577-025-01245-4

Pooja Tiwary, Krishil Oswal, Ryan Varghese, Harsh Anchan

引用次数: 0

Potential of platelet-rich plasma-derived exosomes for bone-defect repair: in vitro and in vivo study. 富血小板血浆源性外泌体骨缺损修复的潜力：体外和体内研究。

IF 3.4 3区生物学

Human Cell Pub Date : 2025-06-10 DOI: 10.1007/s13577-025-01244-5

Xuepu Zhang, Limin Yang, Yaguang Wang, Zhaoliang Ban, Xiaohu Ma, Qiangqiang Zhang, Yue Zhang

{"title":"Potential of platelet-rich plasma-derived exosomes for bone-defect repair: in vitro and in vivo study.","authors":"Xuepu Zhang, Limin Yang, Yaguang Wang, Zhaoliang Ban, Xiaohu Ma, Qiangqiang Zhang, Yue Zhang","doi":"10.1007/s13577-025-01244-5","DOIUrl":"10.1007/s13577-025-01244-5","url":null,"abstract":"Skeletal progenitor-enriched cells, also referred to as skeletal stem cell (SSC)-like cells, are multipotent progenitors that underpin advancements in bone bioengineering and regenerative therapy. Platelet-rich plasma-derived exosomes (PRP-EXOs) exhibit promising capabilities for enhancing osteogenic repair and addressing bone-defect challenges. Nonetheless, whether PRP-EXOs promote bone regeneration and repair by affecting SSC-like cells remains unclear. This study aimed to investigate the effects of PRP-EXOs on SSC-like cells and bone-defect regeneration and repair. PRP-EXOs were purified from rat-derived PRP, and SSC-like cells were obtained via collagenase digestion of femoral and tibial bone tissues. PRP-EXOs were identified by transmission electron microscopy, nanoparticle tracking analysis, and western blotting. The effects of PRP-EXOs on cell viability, proliferation, migration, and osteogenic differentiation were also assessed. Femoral bone-defect models were constructed in rats and evaluated for bone regeneration and repair using microcomputed tomography, bone parameter analysis, and histological assessment. We successfully extracted PRP-EXOs and isolated SSC-like cells from Sprague Dawley rats. Incubation with PRP-EXOs increased cell viability, promoted cell proliferation and migration, and strengthened the osteogenic differentiation of SSC-like cells. Furthermore, PRP-EXO treatment reduced weight loss and accelerated new bone formation and repair in rats with femoral bone defects, accompanied by improvements in bone mineralization and collagen formation. The promotion of bone-defect repair by PRP-EXOs may depend on their promoting effects on the proliferation, migration, and osteogenic differentiation of SSC-like cells, suggesting that PRP-EXOs may be important in bone-defect treatment.","PeriodicalId":49194,"journal":{"name":"Human Cell","volume":"38 4","pages":"117"},"PeriodicalIF":3.4,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144259238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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