ITGA5 drives angiogenesis in diabetic retinopathy via TAK-1/NF-kB activation.

IF 3.4 3区生物学 Q3 CELL BIOLOGY

Human Cell Pub Date : 2025-05-23 DOI:10.1007/s13577-025-01233-8

Feifei Kan, Di Wang, Sijia Li, Yi Gao, Jianwen Wang

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引用次数: 0

Abstract

Diabetic retinopathy is a retinal damage, which causes vision impairment and blindness. Integrin Subunit Alpha 5 (ITGA5) regulates angiogenic response, but its roles in diabetic retinopathy remain unclear. In this work, diabetes mellitus was induced in rats by streptozotocin. ITGA5 interference was achieved by intravitreal delivery of adeno-associated virus. Upregulation of ITGA5 was found in diabetic rat retinal tissues. ITGA5 knockdown decreased the neovascularization, acellular capillary formation, and pericytes. The protein expression of vascular endothelial growth factor (VEGFA), vascular adhesion molecule-1(VCAM-1), and intercellular adhesion molecule-1 (ICAM-1) was reduced after ITGA5 interference. Besides, ITGA5 knockdown decreased the phosphorylation level of FAK, TAK-1, and p65. In vitro, rat retinal microvascular endothelial cells (RRMECs) were cultured under high glucose condition to stimulate diabetic environment. ITGA5 knockdown inhibited VEGFA secretion, tube formation, cell invasion, and migration. Upregulation of VCAM-1 and ICAM-1 that induced by high glucose was reversed by ITGA5 silencing. ITGA5 knockdown blocked the activation of TAK-1/NF-kB pathway in RRMECs. Additionally, in oxygen-induced retinopathy model, ITGA5 interference inhibited pathological neovascularization. These results demonstrate that ITGA5 contributes to the angiogenesis in diabetic retinopathy by activating TAK-1/NF-kB pathway.

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ITGA5通过TAK-1/NF-kB激活驱动糖尿病视网膜病变血管生成。

糖尿病视网膜病变是一种视网膜损伤，会导致视力受损和失明。整合素亚单位α 5 （ITGA5）调节血管生成反应，但其在糖尿病视网膜病变中的作用尚不清楚。本实验采用链脲佐菌素诱导大鼠糖尿病。通过玻璃体内递送腺相关病毒实现ITGA5干扰。糖尿病大鼠视网膜组织中发现ITGA5表达上调。ITGA5敲低可降低新生血管形成、脱细胞毛细血管形成和周细胞。ITGA5干扰后，血管内皮生长因子（VEGFA）、血管粘附分子-1（VCAM-1）、细胞间粘附分子-1（ICAM-1）蛋白表达降低。此外，ITGA5敲除降低了FAK、TAK-1和p65的磷酸化水平。在体外高糖环境下培养大鼠视网膜微血管内皮细胞（RRMECs）以刺激糖尿病环境。ITGA5敲低抑制VEGFA分泌、小管形成、细胞侵袭和迁移。高糖诱导的VCAM-1和ICAM-1上调被ITGA5沉默逆转。ITGA5敲低可阻断rrmes中TAK-1/NF-kB通路的激活。此外，在氧诱导视网膜病变模型中，ITGA5干扰可抑制病理性新生血管。这些结果表明，ITGA5通过激活TAK-1/NF-kB通路参与糖尿病视网膜病变的血管生成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Human Cell CELL BIOLOGY-

CiteScore

5.90

自引率

2.30%

发文量

176

审稿时长

4.5 months

期刊介绍： Human Cell is the official English-language journal of the Japan Human Cell Society. The journal serves as a forum for international research on all aspects of the human cell, encompassing not only cell biology but also pathology, cytology, and oncology, including clinical oncology. Embryonic stem cells derived from animals, regenerative medicine using animal cells, and experimental animal models with implications for human diseases are covered as well. Submissions in any of the following categories will be considered: Research Articles, Cell Lines, Rapid Communications, Reviews, and Letters to the Editor. A brief clinical case report focusing on cellular responses to pathological insults in human studies may also be submitted as a Letter to the Editor in a concise and short format. Not only basic scientists but also gynecologists, oncologists, and other clinical scientists are welcome to submit work expressing new ideas or research using human cells.