Respiratory Research最新文献

{"title":"EEG spectral analysis of nighttime sleep and daytime MSLTs and neurocognitive evaluations in subjects with co-morbid insomnia and OSA.","authors":"Yuan Shi, Yuru Nie, Fengyi Hao, Xujun Feng, Ye Zhang, Larry D Sanford, Rong Ren, Xiangdong Tang","doi":"10.1186/s12931-025-03193-x","DOIUrl":"https://doi.org/10.1186/s12931-025-03193-x","url":null,"abstract":"<p><strong>Background: </strong>Chronic insomnia and obstructive sleep apnea commonly co-occur. Few studies have explored the neurophysiological and neurocognitive characteristics of COMISA, which could help guide improving treatment diagnostic tools and determining novel therapeutic targets. This study aims to explore the neurophysiological and neurocognitive characteristics of COMISA using electroencephalographic (EEG) spectral analysis and subjective and objective neurocognitive measurements.</p><p><strong>Methods: </strong>Participants were from our community recruited OSA-insomnia-COMISA cohort with 206 included for our current analysis including 74 chronic insomniacs (CIs), 55 OSA patients and 77 COMISA patients. Standard polysomnography (PSG) and multiple sleep latency tests (MSLTs) were recorded and used to obtain relative EEG spectral power in each sleep stage during PSG and each session during MSLTs. A series of subjective and objective neurocognitive tests were conducted to evaluate executive function, attention, retrospective and prospective memory and meta-cognition.</p><p><strong>Results: </strong>In PSG and MSLTs, COMISA patients showed combined EEG power characteristics of both CIs and OSA. Specifically, COMISA patients exhibited similar EEG spectral characteristics to CIs, with decreased delta and increased alpha and beta power in NREM sleep stages, and increased beta power in REM and MSLTs. Similar to the EEG spectral power profile of OSA, COMISA patients showed increased delta power in REM and MSLTs. Compared to OSA patients, COMISA patients exhibited worse subjectively measured attention and meta-cognition related to negative beliefs about uncontrollability and danger of worry (NEG), which were positively associated with ISI scores.</p><p><strong>Conclusions: </strong>The EEG spectral power characteristics of COMISA patients in overnight PSG and daytime MSLT appear to be the manifestation of elements of both CIs and OSA. However, the neurocognitive features of COMISA patients in subjectively measured attention and NEG meta-cognition were primarily affected by chronic insomnia.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"139"},"PeriodicalIF":5.8,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144053136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MFGE8 regulates the EndoMT of HLMECs through the BMP signaling pathway and fibrosis in acute lung injury.","authors":"Qingqiang Shi, Huang Liu, Hanghang Wang, Ling Tang, Qi Di, Daoxin Wang","doi":"10.1186/s12931-025-03215-8","DOIUrl":"https://doi.org/10.1186/s12931-025-03215-8","url":null,"abstract":"<p><strong>Background: </strong>To investigate the effects and mechanisms of MFGE8 on LPS-induced endothelial-to-mesenchymal transition (EndoMT) and pulmonary fibrosis in human lung microvascular endothelial cells (HLMECs) and a mouse model of acute lung injury.</p><p><strong>Methods: </strong>Serum MFGE8 levels were compared between ARDS patients and controls. In vitro, HLMECs were treated with LPS, siRNA targeting MFGE8, and recombinant human MFGE8 (rhMFGE8).HLMEC morphology, invasion, migration, and EndoMT markers (CD31, ɑ-SMA) were evaluated. BMP/Smad1/5-Smad4 signaling and Snail expression were assessed via immunofluorescence, western blotting, and qRT-PCR. In vivo, rhMFGE8 effects on pulmonary fibrosis and EndoMT were analyzed in a mouse model of acute lung injury.</p><p><strong>Results: </strong>MFGE8 levels were significantly reduced in ARDS patients, with higher levels correlating to better survival. In vitro, rhMFGE8 improved HLMEC morphology, reduced invasion and migration, and attenuated LPS-induced EndoMT by increasing CD31 and decreasing α-SMA. MFGE8 knockdown increased BMP/Smad1/5-Smad4 signaling and Snail expression, while rhMFGE8 inhibited these effects. In vivo, rhMFGE8 ameliorated pulmonary fibrosis and EndoMT in mice.</p><p><strong>Conclusions: </strong>MFGE8 regulates LPS-induced EndoMT in HLMECs via the BMP/Smad1/5-Smad4 pathway and protects against pulmonary fibrosis in acute lung injury, suggesting it as a therapeutic target for ALI and ARDS.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"142"},"PeriodicalIF":5.8,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995649/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Combination of betulinic acid and EGFR‑TKIs exerts synergistic anti‑tumor effects against wild‑type EGFR NSCLC by inducing autophagy‑related cell death via EGFR signaling.","authors":"Han Wang, Xiaohui Du, Wenwen Liu, Congcong Zhang, Ying Li, Jingwen Hou, Yi Yu, Guiru Li, Qi Wang","doi":"10.1186/s12931-025-03223-8","DOIUrl":"https://doi.org/10.1186/s12931-025-03223-8","url":null,"abstract":"","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"145"},"PeriodicalIF":5.8,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144055364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antigravity muscle density on computed tomography and health-related independence in normal weight patients with chronic obstructive pulmonary disease.","authors":"Satoru Terada, Naoya Tanabe, Tomoki Maetani, Yusuke Shiraishi, Kunihiko Terada, Hiroshi Shima, Tsuyoshi Oguma, Ryo Sakamoto, Megumi Kanasaki, Izuru Masuda, Atsuyasu Sato, Susumu Sato, Toyohiro Hirai","doi":"10.1186/s12931-025-03211-y","DOIUrl":"https://doi.org/10.1186/s12931-025-03211-y","url":null,"abstract":"<p><strong>Background: </strong>Low body mass index (BMI) is a prognostic factor, and skeletal muscle adiposity may affect mortality irrespective of BMI in patients with chronic obstructive pulmonary disease (COPD). However, the association between muscle adiposity and healthy life expectancy in normal-weight patients remains unestablished.</p><p><strong>Objective: </strong>To examine whether lower chest computed tomography (CT)-assessed erector spinae muscle density (ESMD), which represents antigravity muscle adiposity, is associated with subsequent loss of health-related independence in normal-weight patients with COPD.</p><p><strong>Methods: </strong>The ESMD lower limit of normal (LLN) was determined in 194 healthy subjects undergoing lung cancer screening CT. In a prospective cohort of patients with COPD undergoing baseline inspiratory/expiratory CT, the onset of loss of health-related independence, requiring long-term nursing facility or home nursing/medical care, was recorded over 5 years.</p><p><strong>Results: </strong>Smokers with COPD (n = 199) were divided into 4 groups on the basis of BMI and the ESMD-LLN: underweight (n = 22), normal-weight with (n = 40) and without (n = 81) low ESMD, and overweight (n = 56). Greater airway wall thickening was associated with BMI-independent low ESMD. A multivariable Cox proportional hazards model including only normal-weight patients with COPD (n = 121) indicated that low ESMD was independently associated with a higher loss-of-independence rate after adjusting for FEV₁, COPD assessment test score, and a smaller cross-sectional area of erector spinae muscles (hazard ratio [95% confidence interval] = 3.21 [1.30-7.89]).</p><p><strong>Conclusion: </strong>Low antigravity muscle density could reflect airway wall thickening and shorten healthy life expectancy in normal-weight patients with COPD.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"143"},"PeriodicalIF":5.8,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11995616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144046837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nomogram model based on clinical and 3D-EIT parameters for CTEPH diagnosis.","authors":"Jian Xu, Yuhan Wang, Ying Gong, Lu Wang, Yuanlin Song, Xu Wu","doi":"10.1186/s12931-025-03206-9","DOIUrl":"https://doi.org/10.1186/s12931-025-03206-9","url":null,"abstract":"<p><strong>Background: </strong>Chronic thromboembolic pulmonary hypertension (CTEPH) is easily misdiagnosed. Three-dimensional (3D) electrical impedance tomography (EIT) can monitor the whole-lung perfusion at the bedside. In this study, three-dimensional electrical impedance tomography (3D-EIT) features in patients with suspected chronic thromboembolic pulmonary hypertension (CTEPH) was investigated, and nomogram models based on clinical and 3D-EIT parameters were constructed to identify CTEPH.</p><p><strong>Methods: </strong>Patients with pulmonary hypertension (PH) due to left heart disease and chronic hypoxia were excluded. The enrolled patients were divided into CTEPH and Non-CTEPH groups by confirmatory tests. Then, history and laboratory results were collected and 3D-EIT examination was performed. Out of 70 enrolled patients, 50 cases were used as the training set to construct the nomogram model. Obtained nomogram diagnostic model was calibrated and then evaluated using receiver operating characteristic (ROC) curves, decision curve analysis (DCA), and clinical impact curves (CIC).</p><p><strong>Results: </strong>Through a comprehensive univariate analysis, Wald test, Akaike information criterion (AIC), and Bayesian information criterion (BIC), the nomogram model for CTEPH diagnosis based on 50 patients was constructed using venous thromboembolism (VTE) history, D-dimer, maximum of corresponding regional ventilation/perfusion ratio (V/Qmax), range between the maximum and minimum values of regional perfusion (P-Range) and the percentage of ventilation/perfusion match area (VQMatch). The C-index of the nomogram model in the training set was 0.926 (95% CI: 0.859-0.993). In the training set and test set, the nomogram model had a larger area under the curve (AUC) than models containing only VTE history, VTE history + D-dimer and EIT parameters. Both DCA and CIC analyses indicate that this model can provide significant clinical benefits.</p><p><strong>Conclusions: </strong>A nomogram model combining clinical and 3D-EIT parameters facilitated the diagnosis of CTEPH.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"137"},"PeriodicalIF":5.8,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993967/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dehydrocorydaline attenuates bleomycin-induced pulmonary fibrosis by inhibiting fibroblast activation.","authors":"Jianhan He, Huihui Yue, Shufei Zhang, Ruihan Dong, Fengqin Zhang, Xuewen Wang, Ke Wang, Huilan Zhang, Danlei Yang, Zhaoxing Dong, Huiguo Liu","doi":"10.1186/s12931-025-03218-5","DOIUrl":"https://doi.org/10.1186/s12931-025-03218-5","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary fibrosis (PF) is an irreversible, progressive, chronic and fatal interstitial lung disease with limited therapeutic options. Dehydrocorydaline (DHC), derived from the traditional Chinese medicinal plant Corydalis yanhusuo, has exhibited a variety of pharmacological properties. Nevertheless, the potential function and mechanism of DHC in the management of PF have yet to be elucidated.</p><p><strong>Purpose: </strong>To evaluate the therapeutical efficacy of DHC in different PF models and elucidate its underlying mechanism.</p><p><strong>Methods: </strong>A well-established Bleomycin-induced PF mouse model and human precision-cut lung slices (hPCLS) following fibrosis-inducing cocktail stimulation were employed. The antifibrotic effects of DHC on PF were measured by histopathological manifestation, immunofluorescent staining and expression levels of fibrosis related markers. Human primary pulmonary fibroblasts (HPFs) were used to explore the impact of DHC on fibroblast function and the underlying mechanism.</p><p><strong>Results: </strong>Here, we demonstrated that DHC exhibited a therapeutic efficacy in Bleomycin-induced PF mouse model with a dose dependent, as well as in hPCLS after fibrosis-inducing cocktail stimulation, as evidenced by histopathological staining, decrease of Fibronectin, Collagen 1 and α-SMA expression. Additionally, in vitro experiments indicated that DHC effectively suppressed fibroblast to myofibroblast transition, but had no significant effect on the proliferation and migration of fibroblast. Mechanistic studies revealed that the inhibitory effect of DHC on fibroblast activation was dependent on the endoplasmic reticulum stress, thereby inhibiting TGF-β/SMAD signal pathway.</p><p><strong>Conclusions: </strong>Our study implied that DHC hold a promise therapeutic approach against PF by suppressing fibroblast activation. The safety and efficacy of DHC have been preliminary demonstrated in a mouse model.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"136"},"PeriodicalIF":5.8,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992754/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144036258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of radiosensitivity in non-small cell lung cancer based on computed tomography and tumor genomics: a multiple real world cohort study.","authors":"Peimeng You, Qiaxuan Li, Yu Lei, Chuhao Xu, Daipeng Xie, Lintong Yao, Jiaxin Yuan, Junyu Li, Haiyu Zhou","doi":"10.1186/s12931-025-03202-z","DOIUrl":"https://doi.org/10.1186/s12931-025-03202-z","url":null,"abstract":"<p><strong>Background: </strong>The varying degrees of radiotherapy sensitivity of tumors limit the efficacy of tumor radiotherapy. In this research, based on single cell sequence data we used radiomics to help identify and screen feature signatures to distinguish varying radiosensitivity in different regions of the target area of non-small cell lung cancer can provide a new pattern to assess sensitivity of radiotherapy and assist in clinical decision-making.</p><p><strong>Methods: </strong>This retrospective study included CT radiology data from 454 patients diagnosed with non-small cell lung cancer in multiple real-world cohorts prior to radiotherapy. The tumor primary target area was delineated on a training set (n = 154) and segmented to obtain a radiogenomic single signature. The radiogenomic signature LCDigital-RT, which can predict radiosensitivity, was developed by combining transcriptome sequencing signature radiosensitivity index and validated on two independent external validation sets (n = 74) and (n = 160). Besides, we also described the single-cell landscape of non-small cell lung cancer with different radiosensitivity, attempting to explain the potential biological mechanism at the single-cell level.</p><p><strong>Results: </strong>By constructing solely from the single radiomics feature signature, pre LCDigital-RT can effectively identify populations with differences in radiation sensitivity in non-small cell lung cancer, with AUCs of 0.759, 0.728 and 0.745 for the training and two external validation sets, respectively. However, LCDigital-RT has a greater advantage, with a training set AUC of 0.837, which has been well validated in the JXCH cohort (AUC = 0.789) and GDPH cohort (AUC = 0.791). With the help of LCDigital-RT, patients can be divided into radiation sensitive and radiation resistant groups, and there is a significant difference in the characteristics of primary tumor lesions between the two groups. We have also enriched the interpretability of our radiogenomic features in biology at the single-cell level, demonstrating their enormous value in clinical translational research.</p><p><strong>Conclusions: </strong>We have developed an LCDigital RT prediction tool that will help predict populations at risk of radiation sensitivity differences. By visualizing the thermal map of the primary tumor area, we can assist in the development of radiotherapy plans, reduce the occurrence of radiation toxicity events, and improve radiotherapy efficacy. At the same time, it provides a reference basis for evaluating radiation sensitivity from imaging, genetics, and other aspects.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"134"},"PeriodicalIF":5.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MMP12 deficiency attenuates menthol e-cigarette plus house dust-mite effects on pulmonary iron homeostasis and oxidative stress.","authors":"Rakeysha I Pinkston, Matthew Schexnayder, Zakia Perveen, Ingeborg M Langohr, Tomislav Jelesijevic, Arthur L Penn, Alexandra Noël","doi":"10.1186/s12931-025-03213-w","DOIUrl":"https://doi.org/10.1186/s12931-025-03213-w","url":null,"abstract":"<p><strong>Background: </strong>Little is known regarding the pulmonary effects induced by the inhalation of menthol-flavored e-cigarette aerosols on asthma exacerbation, despite the popularity of these devices and flavors among youth and young adults. In the lungs, matrix metalloproteinase 12 (MMP12) expressed and secreted by both alveolar macrophages and bronchial epithelial cells plays an essential role in airway remodeling, a key feature of severe asthma. In this study, we investigated the role of MMP12 in menthol-flavored e-cigarette aerosol exposures plus house-dust mite (HDM)-induced asthmatic responses.</p><p><strong>Methods: </strong>We exposed wild-type (WT) and MMP12 knockout (KO) juvenile female mice to well-characterized menthol-flavored e-cigarette aerosols followed by either PBS or HDM treatment, and evaluated pulmonary outcomes in terms of iron metabolism, oxidative stress responses and pulmonary inflammation.</p><p><strong>Results: </strong>We found high levels of iron in the menthol-flavored e-cigarette aerosol. This correlated with e-cigarette + HDM WT mice exhibiting disruption of pulmonary iron metabolism, suggesting a defense mechanism against iron-mediated toxicity. This was evidenced by altered lung protein concentrations of ferroportin, ferritin, lactoferrin, and transferrin, activation of the antioxidant response element (ARE) pathway and up-regulated expression of NQO1 in e-cigarette + HDM WT mice. Further, despite decreased neutrophilic inflammation, MUC5AC, an oxidative stress inducible mucin, was increased in the e-cigarette + HDM WT mice. In contrast, MMP12 KO mice were protected against iron-induced oxidative stress responses, highlighting a crucial role of MMP12 in this model.</p><p><strong>Conclusion: </strong>These findings revealed in vivo evidence supporting a crucial role for iron metabolism in nicotine salt iron-rich ENDS aerosol toxicity.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"135"},"PeriodicalIF":5.8,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the influenza vaccine protection in the house dust mite-induced chronic allergic asthma mice model and the evaluation of squalene oil in water emulsion as an adjuvant candidate.","authors":"So Yeon Ahn, Thi Len Ho, Eun-Ju Ko","doi":"10.1186/s12931-025-03209-6","DOIUrl":"https://doi.org/10.1186/s12931-025-03209-6","url":null,"abstract":"<p><strong>Background: </strong>Despite the importance of influenza vaccination in asthma patients, the efficacy of this vaccine in asthma has not been well elucidated. We aimed to compare the efficacy of an influenza vaccine of the asthmatic and control mice. We also evaluated the efficacy of AddaVax™ as an adjuvant candidate, which is equivalent to the MF59 influenza vaccine adjuvant in the elderly.</p><p><strong>Method: </strong>House dust mite extracts were intranasally injected into six-week-old female BALB/c mice to induce chronic allergic asthma. Antibody responses after split-inactivated A/Puerto Rico/8/34 H1N1 influenza vaccination with or without AddaVax™ adjuvant were measured using ELISA. Homologous viral protection was determined by measuring the survival rate, lung inflammation level, and lung virus titer after challenge with the human influenza virus strain A/Puerto Rico/8/1934 H1N1. Antigen-specific T cell responses were determined using flow cytometry.</p><p><strong>Result: </strong>The chronic asthma mice immunized with split-inactivated A/Puerto Rico/8/34 H1N1 influenza vaccine showed significant weight loss and higher lung viral load after homologous influenza infection than naïve vaccinated mice. Antigen-specific IgG, IgG1, and IgG2a production did not differ between the naïve and asthma mice. However, serum HI titer was lower in asthma-vaccinated mice after infection. The application of AddaVax™ to a vaccine for mice with asthma enhanced the efficacy of homologous antiviral protection but elicited eosinophil infiltration in the lungs after homologous influenza virus infection.</p><p><strong>Conclusion: </strong>The immune response after split inactivated A/PR8 vaccine differed between asthma and naïve mice, particularly in terms of antibody activity and T cell populations. This study enhances our understanding of how asthma status may influence the effectiveness of influenza vaccine and offers insights into the AddaVax™-induced eosinophilic inflammation, guiding the development of virus vaccine strategies for both healthy individuals and asthma patients.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"132"},"PeriodicalIF":5.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11984255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143994712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ simulation-based team training reduces incidence of negative events during bronchoscopy. A prospective educational intervention cohort study.","authors":"Andreas Fosli Clausen, Søren Sperling, Rune Dall Jensen, Søren Helbo Skaarup","doi":"10.1186/s12931-025-03205-w","DOIUrl":"https://doi.org/10.1186/s12931-025-03205-w","url":null,"abstract":"<p><strong>Introduction: </strong>While bronchoscopy complications are rare, they can be life-threatening if not quickly managed. This study evaluates the effect of a case-based bronchoscopy simulation training using real-world data on complication incidence and nature.</p><p><strong>Methods: </strong>Based on semi structured interviews with respiratory staff in a bronchoscopy unit a team simulation training case was constructed. It was assessed using the Kirkpatrick framework to measure changes in procedural behavior by the rate of adverse events (level three) as the main outcome. Participants' reactions, changes in stress levels, and patient perspectives (levels one, two, and four) were evaluated via questionnaires.</p><p><strong>Results: </strong>Following the educational intervention, the incidence of any negative events during bronchoscopies was reduced from 62% (38/61) to 41% (26/63), p = 0.019. The most frequent event was oxygen desaturation below 90%, which occurred in 34% of the bronchoscopies before the intervention vs. 11% afterwards, p = 0.002. The participants found the simulation-based training relevant but did not change the perceived level of stress. The patient reported to be less awake (2, IQR 1-5, vs. 5, IQR 3-8), p = 0.02 after the intervention.</p><p><strong>Conclusion: </strong>Incorporation of in-situ simulation-based team-training for crisis management during bronchoscopy alter procedural behavior and significantly reduce the occurrence of adverse events; therefore, it should be integrated into future bronchoscopy training curricula.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"133"},"PeriodicalIF":5.8,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11984036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144041804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}