Respiratory Research最新文献

{"title":"Innovating care for people with sarcoidosis using a machine learning-driven approach.","authors":"Vivienne Kahlmann, Astrid Dunweg, Heleen Kicken, Nick Jelicic, Johanna M Hendriks, Richard Goossens, Marlies S Wijsenbeek, Jiwon Jung","doi":"10.1186/s12931-025-03282-x","DOIUrl":"https://doi.org/10.1186/s12931-025-03282-x","url":null,"abstract":"<p><strong>Introduction: </strong>Understanding patients' everyday experience is essential to improve patient centered care in sarcoidosis. So far, patient perspectives are based on survey- and qualitative research.</p><p><strong>Aim: </strong>We aimed to assess patient-driven perspectives on their care trajectories using a novel machine learning-driven approach (MLD).</p><p><strong>Methods: </strong>We used the largest Dutch sarcoidosis patient platform as the data source of patient stories. The patients' stories were extracted with permission. We applied topic modelling (to generate topics among the posts), and sentiment analysis (to find tone of voice in the topics). To validate the findings, we read the top 50 most relevant posts of each topic. An in-depth patients' disease trajectory map was made.</p><p><strong>Results: </strong>Based on 4969 forum posts, 30 final topics and 10 upper themes were generated, which formed the basis for the \"patient journey-map\" which shows patients' perspective across the care pathway. Important decision moments could be identified, as well as care \"tracks\" at home and hospital and topics associated with positive or negative emotions. Most patients' perspectives were about symptoms (mainly negative sentiment), disease-modifying medication (mainly neutral sentiment), and quality of life (negative, neutral and positive).</p><p><strong>Discussion: </strong>A major part of living with sarcoidosis takes place outside the view of the hospital, but this part often remains invisible. MLD is an innovative approach, providing a comprehensive overview of patients' perspectives on health and care. Integrating, these findings in the design of health care delivery has the potential to improve patient-centered care.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"218"},"PeriodicalIF":5.8,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144334253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paraspinal myosteatosis is associated with COPD: a cross-sectional MRI analysis from the population-based KORA cohort.","authors":"Thierno D Diallo, Stefan Karrasch, Matthias Jung, Annette Peters, Roberto Lorbeer, Christopher L Schlett, Ricarda von Krüchten, Fabian Bamberg, Susanne Rospleszcz, Lena S Kiefer","doi":"10.1186/s12931-025-03297-4","DOIUrl":"10.1186/s12931-025-03297-4","url":null,"abstract":"<p><strong>Background: </strong>Muscle dysfunction in chronic obstructive pulmonary disease (COPD) represents a significant extrapulmonary manifestation. Yet, the role of muscle fat infiltration (myosteatosis) in paraspinal muscles remains incompletely characterized. This study investigated whether paraspinal myosteatosis and its distribution patterns are associated with COPD and pulmonary function.</p><p><strong>Methods: </strong>Within the population-based KORA cohort, 214 participants underwent whole-body magnetic resonance imaging and pulmonary function testing. Paraspinal myosteatosis was quantified by chemical shift-encoded MRI at lumbar vertebra 3 (L3), from which proton density fat fraction (PDFF, in %) maps were derived. Intramyocellular (IMCL) and extramyocellular lipids (EMCL) were determined through voxel-based analysis using validated PDFF thresholds. COPD was defined spirometrically as FEV1/FVC below the lower limit of normal. Associations were examined using multivariable regression models adjusted for age, sex, smoking status, physical activity, and body mass index.</p><p><strong>Results: </strong>Among participants (mean age 58.5 ± 5.8 years, 56.1% male), 24 (11.2%) had spirometrically defined COPD. Participants with COPD showed higher paraspinal PDFF (19.9 ± 7.0% vs. 18.3 ± 7.6%) and lower IMCL/EMCL ratios (1.0 ± 0.4 vs. 1.2 ± 0.6) compared to those without COPD. After adjustment, higher PDFF was independently associated with increased odds of COPD (OR 1.69, 95% CI: 1.01-2.84, p = 0.046), while a higher IMCL to EMCL ratio showed protective associations (OR 0.49, 95% CI: 0.24-1.00, p = 0.050). Both total paraspinal PDFF and EMCL were negatively associated with pulmonary gas exchange capacity (TLCO/VA: β=-0.19, 95% CI: -0.35-0.04, p = 0.016 and β=-0.18, 95% CI: -0.33-0.03, p = 0.022, respectively). Conversely, higher IMCL/EMCL ratios were associated with better gas exchange (TLCO/VA: β = 0.15, 95% CI: 0.01-0.29, p = 0.031).</p><p><strong>Conclusions: </strong>This population-based study demonstrates that while increased total paraspinal muscle fat content is associated with higher COPD risk, its compartmental distribution reveals distinct patterns: A higher proportion of IMCL relative to EMCL shows protective associations, potentially reflecting preserved type I oxidative muscle fiber characteristics. These findings suggest that muscle fat distribution patterns may serve as imaging markers of metabolic adaptation in COPD, offering new perspectives for disease monitoring and therapeutic approaches.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"217"},"PeriodicalIF":5.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12167581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multicenter prospective clinical cohort study of pulmonary cryptococcosis in adult non-HIV-infected patients in a southeastern province of China.","authors":"Meiyan Chen, Shuyang Chen, Meng Wang, Huijuan Wang, Shengyuan Zeng, Liying Zhuang, Guoxiang Lai, Zongyang Yu, Yanjing You, Baosong Xie, Xiujuan Yao, Xiangqi Chen, Lan Lin, Qunying Lin, Yuxiong Hu, Liyu Xu, Xiaohua Li, Xiaohong Chen, Danmei Wu, Gongping Chen, Kaixiong Liu, Hui She, Li Lin, Guoqing Yu, Wen Wen","doi":"10.1186/s12931-025-03283-w","DOIUrl":"10.1186/s12931-025-03283-w","url":null,"abstract":"<p><strong>Background: </strong>This study investigates the clinical presentations, diagnostic approaches, and treatment outcomes of pulmonary cryptococcosis (PC) in non-HIV patients in Fujian Province, and explores the correlation between immunological status and clinical features.</p><p><strong>Methods: </strong>A prospective, multicenter cohort study was conducted from April 2017 to March 2022, involving 234 PC patients from 47 hospitals in nine prefecture-level cities in southeastern China's Fujian Province.</p><p><strong>Results: </strong>The study included 145 male and 89 female PC patients, average age 50.66 ± 14.11 years. Immunological status varied: 115 immunocompetent, 17 with potential immunodeficiency due to certain comorbidities, 69 with mild-to-moderate immunodeficiency, and 33 with severe immunodeficiency. Diabetes mellitus was the most common comorbidity. The prevalence of PC is higher in Eastern Fujian (51.7%). 18.4% of patients were exposed to birds/pigeons droppings prior to admission. 37.6% of patients were asymptomatic. Cough and expectoration were common symptoms. Radiologically, multiple lesions with subpleural and lower lobe involvement were typical. The Cryptococcus capsular antigen (CrAg) test showed a sensitivity of 94.9%. Fluconazole was the primary treatment (87.0%), followed by voriconazole. At final follow-up, 85.4% of patients had recovered or improved.</p><p><strong>Conclusions: </strong>PC incidence in non-HIV-infected adults in Fujian is higher in males. Most patients were immunocompetent and from eastern Fujian, with few significant environmental exposures. Clinical and radiological findings were non-specific, highlighting diagnostic challenges. The CrAg test is a valuable diagnostic tool. Treatment with fluconazole and voriconazole resulted in favorable outcomes.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"216"},"PeriodicalIF":5.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimating hypoxia-induced brain dysfunction and cognitive decline through exhaled breath monitoring.","authors":"Sean W Harshman, Kiersten J Weatherbie, Alena R Veigl, Anne E Jung, Madison A Stoner-Dixon, Aubrianne I Dash, Christopher J Land, Dylan T Slizewski, Eli F Kelley, Jennifer Schwanekamp-Kerr, Timothy Halverson, Christina N Davidson, Christopher W Myers, Kara J Blacker, Jennifer A Martin, Rhonda L Pitsch","doi":"10.1186/s12931-025-03296-5","DOIUrl":"10.1186/s12931-025-03296-5","url":null,"abstract":"<p><strong>Background: </strong>Hypoxia remains a concern for aircrew operating high performance aircraft. Sensing and mitigating hypoxia is a line of active research within the US Air Force and US Navy. It is hypothesized that changes in exhaled breath volatile organic compound content could indicate, not only changes in oxygen saturation (SpO₂), but also brain activity and cognitive function.</p><p><strong>Methods: </strong>On-line exhaled breath monitoring via proton transfer reaction mass spectrometry was used to observe changes in volatile organic compound concentrations during mask-free hypoxic exposures. Additionally, electroencephalography measurements in response to an odd-ball paradigm and cognitive tasks were collected throughout the exposures.</p><p><strong>Results: </strong>The data show hypoxic exposures induced a physiological response including a significant reduction in SpO₂, a decrease in the electroencephalography waveform peak-to-peak amplitude (p < 0.05), a significant increase in psychomotor vigilance test response time, and an increase in perceived symptomatology. Exhaled breath results indicate 19 volatile organic compound features are significantly different between hypoxia and normoxia (p < 0.05) with 13 showing an increase in exhaled breath compared to background measurements (p < 0.05). Linear mixed modeling with stepwise reduction demonstrates 7 of the features are significantly indicative of changes in SpO₂ with 3 and 4 features indicative of changes in brain wave functions and psychomotor vigilance test response times, respectively.</p><p><strong>Conclusions: </strong>The data establish, for the first time, differences in exhaled breath volatile concentrations that indicate changes in cognition derived from hypoxic insult.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"215"},"PeriodicalIF":5.8,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12164151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144295188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the role of HDAC3 as an immunotherapy prognostic biomarker and therapeutic target in advanced non-small cell lung cancer.","authors":"Liyuan Dai, Liling Huang, Lin Li, Le Tang, Yuankai Shi, Xiaohong Han","doi":"10.1186/s12931-025-03275-w","DOIUrl":"10.1186/s12931-025-03275-w","url":null,"abstract":"","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"214"},"PeriodicalIF":5.8,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144286919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis reveals shared deregulated neutrophil responses in COVID-19 and idiopathic pulmonary fibrosis.","authors":"Georgios Divolis, Evgenia Synolaki, Rodoula Tringidou, Argyrios Tzouvelekis, Dimitrios T Boumpas, Panagiotis Skendros, Ioanna-Evdokia Galani","doi":"10.1186/s12931-025-03180-2","DOIUrl":"10.1186/s12931-025-03180-2","url":null,"abstract":"<p><strong>Background: </strong>Coronavirus disease 2019 (COVID-19) is a respiratory disease linked with deregulated immune responses, leading to hyperinflammation, acute respiratory distress syndrome, and pulmonary fibrosis, often with fatal outcomes. Neutrophils play a central role in COVID-19 pathogenesis, with elevated peripheral blood neutrophil counts correlating with disease severity. Despite extensive research, the molecular processes associated with neutrophil hyperactivation in COVID-19 remain elusive.</p><p><strong>Methods: </strong>To investigate the molecular signatures underlying neutrophil-driven pathology, we conducted transcriptome analysis in neutrophils isolated from the peripheral blood of COVID-19 patients versus healthy individuals. To evaluate the specificity of identified neutrophil signatures in COVID-19, we extended our transcriptomic analysis to neutrophils from patients with idiopathic pulmonary fibrosis (IPF), a non-infectious fibrotic lung disease. Additionally, immunofluorescence staining was performed on lung biopsy specimens from IPF patients to validate transcriptomic findings at the tissue level.</p><p><strong>Results: </strong>Our analysis revealed significant transcriptional changes in COVID-19 neutrophils, particularly in pathways involved in immune regulation, inflammation, and antiviral responses. Additionally, pathways associated with autophagy and chromatin remodeling were upregulated, while translation-related processes were suppressed, indicating an increased predisposition for neutrophil extracellular trap (NET) release. This neutrophil transcriptional signature in COVID-19 appears to be associated with the previously reported deregulation of the Activin/Follistatin system in the periphery. Notably, a comparative transcriptomic analysis with neutrophils isolated from IPF patients revealed the induction of substantially overlapping inflammatory processes, suggesting common deregulated responses in COVID-19 and IPF. Consistently, significant NET formation, a hallmark of COVID-19-related inflammation, was observed within lung biopsies from IPF patients.</p><p><strong>Conclusion: </strong>By delineating both shared and disease-specific molecular pathways, our findings validate the critical role of neutrophils in COVID-19 and IPF pathophysiology, highlighting their involvement in balancing the inflammatory response across diverse lung diseases.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"213"},"PeriodicalIF":5.8,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12160113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144276355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causes and MEchanisms foR non-atopic Asthma in Children (CAMERA) study: rationale and protocol.","authors":"Mary Njoroge, Gabriela Pimentel Pinheiro, Cinthia Vila Nova Santana, Hajar Ali, Stephanie Hobbs, Santiago Mena-Bucheli, Natalia Romero-Sandoval, Steven Robertson, Charlotte E Rutter, Donna Davoren, Collin Brooks, Jeroen Douwes, Philip J Cooper, Harriet Mpairwe, Camila A Figueiredo, Alvaro A Cruz, Mauricio L Barreto, Neil Pearce, Lucy Pembrey","doi":"10.1186/s12931-025-03279-6","DOIUrl":"10.1186/s12931-025-03279-6","url":null,"abstract":"<p><strong>Background: </strong>The Causes And MEchanisms foR non-atopic Asthma in children (CAMERA) study was designed to investigate risk factors and mechanisms of non-atopic asthma in children and young adults in Brazil, Ecuador, Uganda, and New Zealand. Initial epidemiological analyses using existing datasets identified and compared risk factors for both atopic and non-atopic asthma. The focus of this paper is the protocol for sample collection and analysis of clinical data on possible non-atopic mechanisms.</p><p><strong>Methods: </strong>In each of the four centres, the CAMERA study will enroll 160 participants aged 10-28 years, equally distributed among atopic asthmatics (AA), non-atopic asthmatics (NAA), atopic non-asthmatics and non-atopic non-asthmatics. Participants will be new recruits or returning World ASthma Phenotypes (WASP) study participants. Phase I consists of skin prick tests to define atopy, a general CAMERA questionnaire that covers respiratory and general health to identify asthma cases, followed by an asthma control questionnaire for asthmatics only. Phase II consists of a stress questionnaire and the following clinical assessments: lung function, nasal cytology, blood sampling, in vitro whole blood stimulation to assess IFN-γ production, hair cortisol concentration, dry air and capsaicin challenges, plus in a subset, cold air challenges. Analyses will compare inflammatory, physiological and clinical parameters across the four groups overall and by country.</p><p><strong>Discussion: </strong>Here, we present the protocol for the CAMERA study, to provide relevant methodological details for CAMERA publications and to allow other centres globally to conduct similar analyses. The findings of this mechanistic multi-centre study will inform new and phenotype-specific prevention and treatment approaches.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"212"},"PeriodicalIF":5.8,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12142945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TRANS: a prediction model for EGFR mutation status in NSCLC based on radiomics and clinical features.","authors":"Zhigang Chen, Huiying Lu, Ao Liu, Jia Weng, Lei Gan, Lina Zhou, Xiao Ding, Shicheng Li","doi":"10.1186/s12931-025-03287-6","DOIUrl":"10.1186/s12931-025-03287-6","url":null,"abstract":"<p><strong>Background: </strong>Early detection of epidermal growth factor receptor (EGFR) is critical for guiding therapeutic decisions in non-small-cell lung cancer (NSCLC). The study aims to develop a predictive model for EGFR mutations with multicohort data.</p><p><strong>Methods: </strong>The study enrolled 254 NSCLC patients of four cohorts: the Affiliated Hospital of Qingdao University (AHQU, n = 54), the Second Affiliated Hospital of Soochow University (SAHSU, n = 78), TCGA-NSCLC (n = 91), and CPTAC-NSCLC (n = 31). Radiomic features were extracted using the LIFEx software. The least absolute shrinkage and selection operator (LASSO) algorithm was utilized to select predictive features of CT radiomics, clinical data, and RNA sequencing, which were evaluated using receiver operating characteristic (ROC) curves. A nomogram was developed by integrating predictive features. Biological functions were analyzed utilizing RNA sequencing data.</p><p><strong>Results: </strong>Eight radiomic features, four clinical features, and seven genomic features were selected to construct distinct signatures. Through internal 5-fold cross-validation, the first two signatures demonstrated notable discrimination capabilities for distinguishing between mutated and wild-type EGFR, resulting in area under the curve (AUC) values of 0.79 (± 0.08) and 0.74 (± 0.06), respectively. The combination of clinical variables and radiomics signature resulted in an increased AUC of 0.84 (± 0.01). This combined model was named TRANS, representing TTF-1, radiomic signature, AE1/AE3, NapsinA, and stage, which uses radiomics and routine immunohistochemistry markers as inputs. High-risk TRANS was observed to be associated with poor overall survival, and showed relationships with high T cell infiltration and response to PD-1 immunotherapy.</p><p><strong>Conclusions: </strong>The TRANS model demonstrated favorable ability in predicting EGFR mutation status in NSCLC, providing a valuable approach for optimizing therapeutic strategies in clinical practice.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"211"},"PeriodicalIF":5.8,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144235691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: EIT guided evaluation of regional ventilation distributions in neonatal and pediatric ARDS: a prospective feasibility study.","authors":"Leon Soltész, Judith Leyens, Marieke Vogel, Thomas Muders, Christian Putensen, Florian Kipfmueller, Till Dresbach, Andreas Mueller, Lukas Schroeder","doi":"10.1186/s12931-025-03292-9","DOIUrl":"10.1186/s12931-025-03292-9","url":null,"abstract":"","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"208"},"PeriodicalIF":5.8,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HNRNPH1 promotes autophagy to inhibit the development of lung adenocarcinoma via the HSP90AB1/MAP1LC3B axis.","authors":"Rong Li, Fen Li, Qian Liu, Xu Wu, Xiaowu Tan","doi":"10.1186/s12931-025-03280-z","DOIUrl":"10.1186/s12931-025-03280-z","url":null,"abstract":"<p><strong>Background: </strong>Lung adenocarcinoma (LUAD) is a prevalent subtype of lung cancer (LC) whose progression is regulated by multiple genes. This study sought to find the impact and mechanism of HNRNPH1 on LUAD.</p><p><strong>Methods: </strong>The expression and role of HSP90AB1, HNRNPH1, and autophagy-related protein MAP1LC3B in LUAD were detected. Additionally, bioinformatics analysis, silencing and overexpression techniques, and in vivo modeling were used to explore the regulatory mechanisms of these proteins in the progression of LUAD.</p><p><strong>Results: </strong>HSP90AB1 showed high expression in LUAD and was linked to a worse prognosis. Overexpression of HSP90AB1 significantly promoted the malignant phenotype of LUAD cells and inhibited MAP1LC3B-mediated autophagy. However, overexpression of HNRNPH1 could reverse the malignant phenotype resulting from HSP90AB1 overexpression and promote MAP1LC3B-mediated autophagy by binding to HSP90AB1 mRNA and inhibiting its protein expression. Animal experiments also revealed that overexpression of HNRNPH1 could inhibit tumor progression by promoting cellular autophagy.</p><p><strong>Conclusions: </strong>We verified the key role of HSP90AB1, HNRNPH1, and MAP1LC3B in LUAD, and revealed a possible regulatory mechanism, namely, HNRNPH1 could inhibit the development of LUAD by promoting autophagy through the HSP90AB1/MAP1LC3B axis. These findings may offer new insights for improving the treatment and prognosis of LUAD.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":49131,"journal":{"name":"Respiratory Research","volume":"26 1","pages":"206"},"PeriodicalIF":5.8,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12139211/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144227316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}