Revista Brasileira De Parasitologia Veterinaria最新文献_第6页

Anthelmintic resistance of gastrointestinal nematodes in cattle in Brazil and Argentina - current status and global perspectives. 巴西和阿根廷牛的胃肠道线虫抗药性--现状与全球前景。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-08-12 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024041

Fernando de Almeida Borges, Alessandro Francisco Talamini do Amarante, Welber Daniel Zaneti Lopes, Candela Canton, Luis Alvarez, Adrian Lifschitz

{"title":"Anthelmintic resistance of gastrointestinal nematodes in cattle in Brazil and Argentina - current status and global perspectives.","authors":"Fernando de Almeida Borges, Alessandro Francisco Talamini do Amarante, Welber Daniel Zaneti Lopes, Candela Canton, Luis Alvarez, Adrian Lifschitz","doi":"10.1590/S1984-29612024041","DOIUrl":"10.1590/S1984-29612024041","url":null,"abstract":"This review outlines the current state of anthelmintic resistance (AHR) of gastrointestinal nematodes (GINs) among cattle in Argentina and Brazil, emphasizing the economic repercussions, animal health and welfare. The analysis explores factors associated with AHR and proposes a potential solution: the use of drug combinations. Both countries are grappling with a severe AHR scenario in cattle, having progressed through incipient, established, and advanced phases, leading to extreme cases of animal mortality due to ineffective control strategies. Genera such as Cooperia and Haemonchus have the highest reports of resistance, with Oesophagostomum radiatum also posing significant problems. While oral benzimidazoles and levamisole remain effective in most herds, moxidectin is entering an advanced resistance phase, and avermectins are increasingly deemed ineffective. The review explores the impact ofclimate, mixed grazing, animal movement and other husbandry practices, and the relationship between ectoparasite control and the emergence of resistant helminths. Notably, the discussion includes the strategic use of drug combinations as a valuable approach to address resistant GINs control in livestock, highlighting its significant potential to mitigate the challenges posed by AHR in the cattle industry of these countries.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 3","pages":"e010524"},"PeriodicalIF":1.3,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141976953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Allergenic response induced by Pterobothrium crassicolle (Cestoda: Trypanorhyncha) extracts in murine model. Pterobothrium crassicolle（绦虫纲：Trypanorhyncha）提取物在小鼠模型中诱导的过敏反应。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024039

Danuza Pinheiro Bastos Garcia de Mattos, Maurício Afonso Verícimo, Sérgio Carmona de São Clemente, Michelle Cristie Gonçalves da Fonseca, Marcelo Knoff

{"title":"Allergenic response induced by Pterobothrium crassicolle (Cestoda: Trypanorhyncha) extracts in murine model.","authors":"Danuza Pinheiro Bastos Garcia de Mattos, Maurício Afonso Verícimo, Sérgio Carmona de São Clemente, Michelle Cristie Gonçalves da Fonseca, Marcelo Knoff","doi":"10.1590/S1984-29612024039","DOIUrl":"10.1590/S1984-29612024039","url":null,"abstract":"The aim of this study was to determine the allergenic activity of components present in crude extracts of Pterobothrium crassicolle plerocerci (CPE) and blastocysts (CBE) obtained from Micropogonias furnieri in a murine model. Two groups of seven animals each received 50 µg of CPE or CBE on days 1, 35 and 120. Serum samples were tested by ELISA and Immunoblotting. Specific IgG and IgE levels were detected by ELISA, showing specific humoral responses for the primary immunization for both immunoglobulins and continuously growing titers for IgE. Positive Passive Cutaneous Anaphylaxis tests in rats sensitized with anti-CBE sera and tested by CBE, showed biologically, the allergenic activity of the extracts. The CPE and CBE showed some different recognition regions but both experimental groups recognized all regions of the extracts when tested for cross reactions, showing that CPE and CBE could share antigenic recognition sites.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 3","pages":"e002624"},"PeriodicalIF":1.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil. 巴西南部被路边捕杀的野生哺乳动物体内没有利什曼原虫 DNA。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024038

Julia Somavilla Lignon, Diego Moscarelli Pinto, Mariana Accorsi Teles, Maira Aparecida Christello Trindade, Priscila Rockenbach Portela, Silvia Gonzalez Monteiro, Kauê Rodriguez Martins, Rodrigo Casquero Cunha, Felipe Geraldo Pappen, Bianca Conrad Bohm, Fábio Raphael Pascoti Bruhn

{"title":"Absence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil.","authors":"Julia Somavilla Lignon, Diego Moscarelli Pinto, Mariana Accorsi Teles, Maira Aparecida Christello Trindade, Priscila Rockenbach Portela, Silvia Gonzalez Monteiro, Kauê Rodriguez Martins, Rodrigo Casquero Cunha, Felipe Geraldo Pappen, Bianca Conrad Bohm, Fábio Raphael Pascoti Bruhn","doi":"10.1590/S1984-29612024038","DOIUrl":"10.1590/S1984-29612024038","url":null,"abstract":"Leishmaniasis are neglected diseases transmitted by vectors that affect domestic and wild animals, including humans. Due to its incidence and lethality, this zoonosis is a worrying public health problem, making it essential to identify all links in the transmission chain. Infection of wild mammals by Leishmania spp. remains poorly understood, especially in southern Brazil. Therefore, the objective was to research, using the PCR technique, the presence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil. Carcasses of 96 animals were collected from highways in the Pelotas microregion, Rio Grande do Sul, southern Brazil and subjected to necropsies. Tissue fragments (spleen, skin, liver, kidney, heart, lung, lymph nodes, bone marrow and blood) were collected and genomic DNA was extracted. PCR protocols targeting the ITS1, kDNA and 18S genes were tested. We found no evidence of Leishmania spp. circulation in the studied population. However, epidemiological studies like this one are of great relevance, as they allow monitoring of the occurrence of pathogens and help identify possible risk areas. As these animals act as epidemiological markers for the presence of the microorganism, studies must be carried out continuously to understand whether there are sources of infection in the region.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e007924"},"PeriodicalIF":1.3,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296681/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141753180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New occurrences, mean infestation intensity and prevalence of parasitic isopods (Isopoda, Cymothoida, Bopyridae) associated with Macrobrachium amazonicum (Decapoda, Palaemonidae) from the mouth of the Amazon River. 亚马逊河河口与大鲎（十足目，鲎科）相关的寄生等足类（等足目，栉水母纲，栉水母科）的新发生率、平均侵扰强度和流行率。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-15 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024037

Sting Silva Duarte, Jô de Farias Lima, Lucio André Viana

{"title":"New occurrences, mean infestation intensity and prevalence of parasitic isopods (Isopoda, Cymothoida, Bopyridae) associated with Macrobrachium amazonicum (Decapoda, Palaemonidae) from the mouth of the Amazon River.","authors":"Sting Silva Duarte, Jô de Farias Lima, Lucio André Viana","doi":"10.1590/S1984-29612024037","DOIUrl":"10.1590/S1984-29612024037","url":null,"abstract":"The Amazon prawn or Macrobrachium amazonicum (Heller, 1862) is widely distributed in South America, occurring in the Orinoco and Amazon rivers, and forms an important source of income for riverside families. This prawn hosts crustacean ectoparasites of the genus Probopyrus (Giard & Bonnier, 1888) (Bopyridae) that infest its gill cavity. The aim of the present study was to report new occurrences of Probopyrus in Amazon prawns caught in the Amazon River. Macrobrachium amazonicum prawns were collected between May 2017 and April 2018, and again from July 2021 to May 2022 in the regions of Ilha de Santana and Rio Mazagão, state of Amapá, Brazil. Among the 5,179 prawn specimens caught, 133 were parasitized by the ectoparasites Probopyrus pandalicola (Packard, 1879), Probopyrus bithynis (Richardson, 1904), Probopyrus floridensis (Richardson, 1904) and Probopyrus palaemoni (Lemos de Castro & Brasil Lima, 1974). These occurrences of P. floridensis and P. palaemoni in M. amazonicum were the first records of this on the northern coast of Brazil. These four ectoparasites are not limited to specific host species or genera, as observed in this study, which reports four species of Probopyrus infesting M. amazonicum.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e000324"},"PeriodicalIF":1.3,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11296680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141628044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tabanidae (Diptera) collected on horses in a Cerrado biome in the state of Tocantins, Brazil. 在巴西托坎廷斯州塞拉多生物群落的马身上采集到的 Tabanidae（双翅目）。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-15 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024036

Mariana Vaz da Costa, Gratchela Dutra Rodrigues, Helena Iris Leite de Lima, Tiago Kütter Krolow, Rodrigo Ferreira Krüger

引用次数: 0

Epidemiology of sarcoptic mange in free-ranging vicuñas (Vicugna vicugna): a cross-sectional study in Andean highland communities in Peru. 在秘鲁安第斯高原社区开展的一项横断面研究：散养骆驼（Vicugna vicugna）的肉毒疥癣流行病学。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-08 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024030

Marcos Enrique Serrano-Martínez, Gabriel Bazán Alcántara, Marco Enciso, Fahrid Huanca Mori, Luis Llanco Albornoz, Stef de Haan, Henry Juárez, Sthefany Aguilar Tejeda, Cristofer Cruz Camero, Cesar Burga-Cisterna

{"title":"Epidemiology of sarcoptic mange in free-ranging vicuñas (Vicugna vicugna): a cross-sectional study in Andean highland communities in Peru.","authors":"Marcos Enrique Serrano-Martínez, Gabriel Bazán Alcántara, Marco Enciso, Fahrid Huanca Mori, Luis Llanco Albornoz, Stef de Haan, Henry Juárez, Sthefany Aguilar Tejeda, Cristofer Cruz Camero, Cesar Burga-Cisterna","doi":"10.1590/S1984-29612024030","DOIUrl":"10.1590/S1984-29612024030","url":null,"abstract":"Sarcoptic mange or scabies is a contagious parasitic skin disease that affects a wide range of domestic and wildlife species. A cross-sectional study was carried out to determine the prevalence, molecular identification, and characteristics of sarcoptic mange in vicuñas. A total of 3,274 vicuñas were examined. Following ritual harvesting events (\"chaccus\") in 13 Andean communities. The presence of mange mites was determined by the skin scraping technique and confirmed by PCR analysis using specific primers for the ITS2 gene of Sarcoptes scabiei. The presence of mange mites was also confirmed by microscopy using samples taken from wallows. A data collection form was used to register the characteristics of the vicuñas sampled. The prevalence of sarcoptic mange was 4.9% (95% CI: 4.1 - 5.6%). All samples from wallows tested positive (9/9). Importantly, the presence of the species S. scabiei was molecularly confirmed. Adult females with regular body condition were more susceptible to sarcoptic mange, although the lesions were mild. This study confirms the presence of S. scabiei in semi-captive vicuñas and points to the possible role of wallows in the dissemination of Sarcoptes mites.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e020523"},"PeriodicalIF":1.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253819/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in dairy cattle from São Paulo State, Brazil. 巴西圣保罗州奶牛犬新孢子虫和弓形虫的血清流行率和风险因素。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-08 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024034

Juliana Correa Bernardes, Fernanda Pinto-Ferreira, Winni Alves Ladeia, Eloiza Teles Caldart, Aline Ticiani Pereira Paschoal, Thais Agostinho Martins, José Victor Pronievicz Barreto, Maria Eduarda Crespi, Luiz Daniel de Barros, Beatriz de Souza Lima Nino, Silvana Gomez Gonzalez, João Luis Garcia

{"title":"Seroprevalence and risk factors for Neospora caninum and Toxoplasma gondii in dairy cattle from São Paulo State, Brazil.","authors":"Juliana Correa Bernardes, Fernanda Pinto-Ferreira, Winni Alves Ladeia, Eloiza Teles Caldart, Aline Ticiani Pereira Paschoal, Thais Agostinho Martins, José Victor Pronievicz Barreto, Maria Eduarda Crespi, Luiz Daniel de Barros, Beatriz de Souza Lima Nino, Silvana Gomez Gonzalez, João Luis Garcia","doi":"10.1590/S1984-29612024034","DOIUrl":"10.1590/S1984-29612024034","url":null,"abstract":"Neospora caninum is a major cause of reproductive loss in cattle worldwide as it leads to abortion and animal repositioning. Although Toxoplasma gondii does not cause a reproductive problem in cattle, consuming raw or uncooked beef poses the risk of transmission. This study aimed to evaluate the occurrence of anti-N. caninum and anti-T. gondii antibodies in dairy cattle in the West and Northwest regions of São Paulo State, Brazil. A total of 653 serum samples from dairy cows were analyzed using an indirect immunofluorescence assay (IFA). Epidemiological data from the farms were associated with the serological results of the animals by logistic regression based on the presence of antibodies. The frequencies of the antibodies against N. caninum and T. gondii were 41.6% (272/653) and 11.5% (75/653), respectively. A statistically significant association was observed between: the serum anti-N. caninum antibodies and breed, history of food supplementation for calves, introduction of outside animals that later presented reproductive problems, and history of reproductive problems by the trimester of gestation. The present study highlights the importance of neosporosis in dairy cattle in the study regions and that the inclusion of this parasite in the investigation of animals with reproductive disorders is important.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e006024"},"PeriodicalIF":1.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253822/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141564923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Feline immunodeficiency virus, feline leukemia virus and Leishmania spp. prevalence in cats from shelters in Mato Grosso do Sul, Brazil. 巴西南马托格罗索州收容所中猫的猫免疫缺陷病毒、猫白血病病毒和利什曼原虫感染率。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024035

Walderson Zuza Barbosa, Karen Araújo Magalhães, Kamily Fagundes Pussi, Manoel Sebastião da Costa Lima Junior, Herintha Coeto Neitzke-Abreu

{"title":"Feline immunodeficiency virus, feline leukemia virus and Leishmania spp. prevalence in cats from shelters in Mato Grosso do Sul, Brazil.","authors":"Walderson Zuza Barbosa, Karen Araújo Magalhães, Kamily Fagundes Pussi, Manoel Sebastião da Costa Lima Junior, Herintha Coeto Neitzke-Abreu","doi":"10.1590/S1984-29612024035","DOIUrl":"10.1590/S1984-29612024035","url":null,"abstract":"Diseases such as those caused by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) represent health problems for cats. Feline leishmaniasis (FL) has been reported in several cities across the country. The objective was to carry out a clinical-epidemiological and laboratory study of FIV, FeLV and FL in cats from shelters in Dourados, Mato Grosso do Sul, Brazil. Blood samples and swabs from the conjunctival and nasal mucosa were obtained from 75 cats, from four animal shelters. Serology for FIV and FeLV was performed. For Leishmania, polymerase chain reaction (PCR) was performed on blood, conjunctiva and nasal mucosa. In the immunochromatographic serological test, seven cats tested positive for FIV and none for FeLV. No samples was positive in PCR for Leishmania. The study showed that despite the presence of human and canine leishmaniasis in the studied region, Leishmania spp. were absent in the cats studied. To avoid an increase in contagion in shelters, it is essential isolate cats with FIV.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e006324"},"PeriodicalIF":1.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First report of unusual case of parasitism by Amblyomma nodosum (Neumann, 1889) in a yellow cururu toad (Rhinella icterica) in the Northeastern Brazilian Caatinga. 首次报告巴西东北部卡廷加地区黄库鲁蟾蜍（Rhinella icterica）寄生 Amblyomma nodosum（Neumann，1889 年）的不寻常病例。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024031

Daniel Antônio Braga Lee, Darci Moraes Barros-Battesti, Paulo Vitor Cadina Arantes, Jovêncio Mateus Sada, Gustavo Seron Sanches, Marcos Rogério André, Victor Fernando Santana Lima

{"title":"First report of unusual case of parasitism by Amblyomma nodosum (Neumann, 1889) in a yellow cururu toad (Rhinella icterica) in the Northeastern Brazilian Caatinga.","authors":"Daniel Antônio Braga Lee, Darci Moraes Barros-Battesti, Paulo Vitor Cadina Arantes, Jovêncio Mateus Sada, Gustavo Seron Sanches, Marcos Rogério André, Victor Fernando Santana Lima","doi":"10.1590/S1984-29612024031","DOIUrl":"10.1590/S1984-29612024031","url":null,"abstract":"The Amblyomma genus (Arachnida: Ixodidae) is widely distributed in South America, with 34 species occurring in Brazil. Amblyomma nodosum Neumann 1889 is a species that predominantly feeds on Passeriformes during immature stages (larvae and nymphs) and anteaters (Myrmecophagidae) during adult stages. The aim of the present study is to report, for the first time, an unusual case of parasitism by adults of A. nodosum on a yellow cururu toad (Rhinella icterica) captured in the city of Nossa Senhora da Glória, Sergipe state (Northeastern Brazil) in the Caatinga biome, and also investigate the presence of DNA of Rickettsia in the collected material. DNA was extracted from all specimens collected (N=8) and subjected to PCR assays based on the tick 16S rRNA endogenous gene and gltA gene for Rickettsia sp. All samples (8/8; 100%) were positive for the 16S rRNA endogenous gene and two amplicons (obtained from one male and one female) were purified and sequenced. The BLASTn analysis of the sequences revealed a high degree of similarity (95-100%) with A. nodosum sequences previously deposited on GenBank, while the phylogenetic analysis clustered the sequences obtained in the same clade as A. nodosum sequences from Brazil.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e005324"},"PeriodicalIF":1.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular identification of new Trypanosoma evansi type non-A/B isolates from buffaloes and cattle in Indonesia. 印度尼西亚水牛和牛中新分离出的伊万斯锥虫非A/B型的分子鉴定。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024033

Didik Tulus Subekti, Lucia Tri Suwanti, Dyah Ayu Kurniawati, Mufasirin Mufasirin, Sunarno Sunarno

{"title":"Molecular identification of new Trypanosoma evansi type non-A/B isolates from buffaloes and cattle in Indonesia.","authors":"Didik Tulus Subekti, Lucia Tri Suwanti, Dyah Ayu Kurniawati, Mufasirin Mufasirin, Sunarno Sunarno","doi":"10.1590/S1984-29612024033","DOIUrl":"10.1590/S1984-29612024033","url":null,"abstract":"Trypanosoma evansi is reportedly divided into two genotypes: types A and B. The type B is uncommon and reportedly limited to Africa: Kenya Sudan, and Ethiopia. In contrast, type A has been widely reported in Africa, South America, and Asia. However, Trypanosoma evansi type non-A/B has never been reported. Therefore, this study aims to determine the species and genotype of the Trypanozoon subgenus using a robust identification algorithm. Forty-three trypanosoma isolates from Indonesia were identified as Trypanosoma evansi using a molecular identification algorithm. Further identification showed that 39 isolates were type A and 4 isolates were possibly non-A/B types. The PML, AMN-SB1, and STENT3 isolates were likely non-A/B type Trypanosoma evansi isolated from buffalo, while the PDE isolates were isolated from cattle. Cladistic analysis revealed that Indonesian Trypanosoma evansi was divided into seven clusters based on the gRNA-kDNA minicircle gene. Clusters 6 and 7 are each divided into two sub-clusters. The areas with the highest genetic diversity are the provinces of Banten, Central Java (included Yogyakarta), and East Nusa Tenggara. The Central Java (including Yogyakarta) and East Nusa Tenggara provinces, each have four sub-clusters, while Banten has three.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 2","pages":"e001324"},"PeriodicalIF":1.3,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0