Revista Brasileira De Parasitologia Veterinaria最新文献_第6页

Single and co-infections by Anaplasma platys, Babesia vogeli, Ehrlichia canis, and Hepatozoon canis in dogs from Rio Grande do Norte, Brazil: frequency and molecular characterization. 巴西北部大德州里约热内卢犬的单次和联合感染：频率和分子特征

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-19 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025067

Bruno Vinicios Silva de Araújo, Isa Lorena Pinto Dantas Bezerra, Gabriela Linhares Leite, Amanda Haisi, João Pessoa Araújo Júnior, Francisco de Assis Leite Souza, Leucio Camara Alves, João Marcelo Azevedo de Paula Antunes, Juliana Fortes Vilarinho Braga

{"title":"Single and co-infections by Anaplasma platys, Babesia vogeli, Ehrlichia canis, and Hepatozoon canis in dogs from Rio Grande do Norte, Brazil: frequency and molecular characterization.","authors":"Bruno Vinicios Silva de Araújo, Isa Lorena Pinto Dantas Bezerra, Gabriela Linhares Leite, Amanda Haisi, João Pessoa Araújo Júnior, Francisco de Assis Leite Souza, Leucio Camara Alves, João Marcelo Azevedo de Paula Antunes, Juliana Fortes Vilarinho Braga","doi":"10.1590/S1984-29612025067","DOIUrl":"10.1590/S1984-29612025067","url":null,"abstract":"This study investigated the presence of Anaplasma platys, Babesia vogeli, Ehrlichia canis, and Hepatozoon canis in dogs in Mossoró, Rio Grande do Norte, Brazil, from October 2022 to August 2023. Blood samples were collected from 181 dogs with suspected hemoparasitoses based on clinical and/or laboratory findings (such as hematological alterations) for direct hemoparasite examination on slides, polymerase chain reaction (PCR), and phylogenetic analysis. PCR revealed that 72.3% of dogs were infected with at least one agent. Among these, 44.7% were monoinfected and 27.6% coinfected, with 21.5% infected by two agents and 6.1% by three or more. E. canis was most frequent (41.9%), followed by H. canis (35.4%), A. platys (21.5%), and B. vogeli (7.7%). Anaplasma and Ehrlichia-like morulae, Hepatozoon sp. gametocytes, and Babesia sp. merozoites were found in 20.0%, 11.1%, 15.0%, and 1.7% of animals, respectively. Phylogenetic analysis of 16S and 18S rRNA gene sequences revealed high similarity to reference strains from Brazil and other countries. The infection patterns underscore the importance of diagnostic and preventive measures to safeguard animal health in areas endemic for Rhipicephalus sanguineus sensu lato (s.l.), given the frequency of coinfections that may exacerbate clinical signs and hinder treatment.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e004825"},"PeriodicalIF":1.2,"publicationDate":"2025-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12822363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Propagation dynamics of Anaplasma marginale in a Brazilian tick cell line (RBME-6) derived from Rhipicephalus microplus. 微小鼻头虫衍生巴西蜱细胞系RBME-6中边缘无原体的繁殖动力学。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-15 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025075

Gustavo Seron Sanches, Ana Carolina Castro-Santiago, Ricardo Bassini-Silva, Ana Cláudia Calchi, Simone Michaela Simons, Luís Antonio Mathias, Pablo Henrique Nunes, Rosangela Zacarias Machado, Marcos Rogério André, Darci Moraes Barros-Battesti

{"title":"Propagation dynamics of Anaplasma marginale in a Brazilian tick cell line (RBME-6) derived from Rhipicephalus microplus.","authors":"Gustavo Seron Sanches, Ana Carolina Castro-Santiago, Ricardo Bassini-Silva, Ana Cláudia Calchi, Simone Michaela Simons, Luís Antonio Mathias, Pablo Henrique Nunes, Rosangela Zacarias Machado, Marcos Rogério André, Darci Moraes Barros-Battesti","doi":"10.1590/S1984-29612025075","DOIUrl":"10.1590/S1984-29612025075","url":null,"abstract":"Anaplasma marginale is an obligate intraerythrocytic alpha-proteobacterium that causes bovine anaplasmosis, which is responsible for economic losses in the livestock industry worldwide. Although the mechanism of A. marginale infection in tick cell lines has been extensively studied, replication dynamics have not been investigated so far. This study presents the replication kinetics of A. marginale in the RBME-6 tick cell line derived from Rhipicephalus microplus over time. Cell cultures were infected with corpuscles of A. marginale isolated from bovine blood. The replication curve was generated by the number of copies of the msp1β gene obtained by reverse-transcription quantitative PCR (RT-qPCR) using complementary DNA (cDNA) synthesized from RNA. An initial lag phase and an exponential replication phase were recorded. A subsequent death phase was observed, with rapidly decreasing numbers of A. marginale msp1β copies. The surviving population of A. marginale entered a long-term stationary phase from 13 dpi until 29 dpi. Light microscopy was used to monitor the infection in the cells. The propagation kinetics of A. marginale in RBME-6 cells were shown to be essential for guiding future studies aimed at unraveling the crosstalk between this bacterium and its biological tick vector.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e007025"},"PeriodicalIF":1.2,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemical profile and antiparasitic effects of Lippia origanoides essential oil against Rhipicephalus microplus, Haemonchus contortus, and Caenorhabditis elegans. 牛皮精油对微小鼻头虫、弯曲血蜱和秀丽隐杆线虫的化学特征及抗寄生作用。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-12 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025072

Joelson Gomes de Oliveira, Caio Pavão Tavares, Tássia Lopes do Vale, Dauana Mesquita-Sousa, Gabriel Sousa Brito, Márcia Aldeany Almeida de Sousa, José Fábio França Orlanda, Odair Dos Santos Monteiro, Wesley Douglas Ribeiro, Lívio Martins Costa-Junior, Francisco Eduardo Aragão Catunda Júnior, José Roberto Pereira de Sousa

{"title":"Chemical profile and antiparasitic effects of Lippia origanoides essential oil against Rhipicephalus microplus, Haemonchus contortus, and Caenorhabditis elegans.","authors":"Joelson Gomes de Oliveira, Caio Pavão Tavares, Tássia Lopes do Vale, Dauana Mesquita-Sousa, Gabriel Sousa Brito, Márcia Aldeany Almeida de Sousa, José Fábio França Orlanda, Odair Dos Santos Monteiro, Wesley Douglas Ribeiro, Lívio Martins Costa-Junior, Francisco Eduardo Aragão Catunda Júnior, José Roberto Pereira de Sousa","doi":"10.1590/S1984-29612025072","DOIUrl":"10.1590/S1984-29612025072","url":null,"abstract":"Growing antiparasitic resistance challenges parasite control in livestock, increasing interest in natural product-based alternatives for animal health and food quality. This study evaluated the in vitro activity of Lippia origanoides essential oil against Rhipicephalus microplus, Haemonchus contortus, and Caenorhabditis elegans. Fresh leaves of L. origanoides from Maranhão, Brazil, were used to obtain essential oil by hydrodistillation, and its chemical composition was determined by gas chromatography-mass spectrometry (GC-MS). Bioassays evaluated activity against R. microplus larvae (immersion and repellency), H. contortus eggs (hatch inhibition), and C. elegans adults (mortality), all using serial dilutions of essential oil. Experiments were performed in nine replicates, and results were analyzed by nonlinear regression. Chemical analysis of the oil revealed the main constituents: camphor (32.13%), β-bisabolene (10.02%) and camphene (6.66%). The larval immersion test of the oil showed an LC50 (letal concentration) of 7.48 mg/mL against ticks. A repellency assay revealed an RC50 (repellent concentration) of 0.058 mg/cm2 at 10 minutes. The egg hatch assay for H. contortus resulted in an LC50 of 0.67 mg/mL, whereas the adult mortality assay for C. elegans showed an LC50 of 2.23 mg/mL. These results suggest the acaricidal, repellent, and nematicidal efficacy of essential oil of L. origanoides.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e014825"},"PeriodicalIF":1.2,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative analysis of radiological and serological methods for diagnosis of cystic echinococcosis. 囊性包虫病放射学与血清学诊断方法的比较分析。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-12 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025073

Baris Can, Busra Betul Ozmen Capin, Meltem Kursun, Canan Cimsit, Aysegul Karahasan Yagci

{"title":"Comparative analysis of radiological and serological methods for diagnosis of cystic echinococcosis.","authors":"Baris Can, Busra Betul Ozmen Capin, Meltem Kursun, Canan Cimsit, Aysegul Karahasan Yagci","doi":"10.1590/S1984-29612025073","DOIUrl":"10.1590/S1984-29612025073","url":null,"abstract":"Cystic echinococcosis (CE) diagnosis relies on clinical signs, serological tests, radiological findings and histopathology. Lack of pathognomonic features or clinician inexperience can make CE diagnosis challenging. In this study, we aimed to investigate the demographic, serological, and radiological characteristics of individuals with a preliminary diagnosis of CE. A total of 1,410 serum samples from 1,265 patients were analysed for the presence of IgG antibodies Anti-Echinococcus granulosus IgG using chemiluminescence immunoassay (CLIA) and indirect haemagglutination assay (IHA). Among these, 148 samples showed serological and radiological findings compatible with CE. Echinococcal cysts were confirmed by ultrasonography, computed tomography, or magnetic resonance imaging. Among the 148 patients compatible with CE, 88 were women (59.5%) with a mean age of 45.0 ± 19.1 years, and 60 were men with a mean age of 35.4 ± 18.3 years. Although there was no significant difference between the sexes regarding the number, location, size, or complications, the mean age of female patients was higher than that of male patients. Our findings suggest that radiological methods are essential for assessing cyst size and activity, while serological methods provide complementary diagnostic value and contribute to generating important epidemiological data. As CE is a zoonosis, collaboration with veterinary surveillance is essential for effective control.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e014125"},"PeriodicalIF":1.2,"publicationDate":"2025-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704771/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145795204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitosis caused by Calyptospora in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Sacaizal Lake in the state of Amapá, Brazil. 巴西amapap<e:1>州Sacaizal湖水蚤Calyptospora引起的寄生虫病。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-08 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025070

Nayana Moraes de Sena, Jhonata Eduard, Camila Maria Barbosa Pereira, Maria do Perpetuo Socorro Progene Vilhena, José Francisco Berrêdo Reis da Silva, José Ledamir Sindeaux Neto, Michele Velasco

{"title":"Parasitosis caused by Calyptospora in Pygocentrus nattereri (Characiformes: Serrasalmidae) from the Sacaizal Lake in the state of Amapá, Brazil.","authors":"Nayana Moraes de Sena, Jhonata Eduard, Camila Maria Barbosa Pereira, Maria do Perpetuo Socorro Progene Vilhena, José Francisco Berrêdo Reis da Silva, José Ledamir Sindeaux Neto, Michele Velasco","doi":"10.1590/S1984-29612025070","DOIUrl":"10.1590/S1984-29612025070","url":null,"abstract":"Parasites of the phylum Apicomplexa are among the main etiological agents of diseases in vertebrates and invertebrates, including humans and domestic animals. In the present study, 18 specimens of Pygocentrus nattereri (red piranha) captured in Lagoa do Sacaizal, municipality of Pracuúba, state of Amapá, were analyzed. Of these, 6 (33%) had whitish spots on their liver lobes. Clusters of Calyptospora sp. oocysts measuring 18.1 ± 0.5 (17.4-18.7) µm in diameter, with four pyriform sporocysts measuring 9.0 ± 0.45 (8.3-9.8) µm in length and 4.3 ± 0.50 (3.7-5.1) µm in width, each with two sporozoites. Histopathological evaluation indicated the presence of oocysts adjacent to blood vessels and in the hepatopancreas, promoting necrosis, degeneration, and tissue fibrosis associated with inflammatory infiltrate and melanomacrophage bodies. This is the first record of coccidia parasitizing P. nattereri.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e011125"},"PeriodicalIF":1.2,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145726799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coprological assessment of domestic carnivores in public areas and health education focused on larva migrans in São Paulo State, Brazil. 巴西<s:1>圣保罗州公共区域家养食肉动物的生态学评估和以幼虫迁徙为重点的健康教育。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-08 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025071

Abraão Isaque da Silva, Adrieli Leite Araujo Moura, Beatriz Maia Galetti, Bruna Larocca Vieira, Camila Vitória Dias da Silva, Giovana Almeida Reis, Letícia Leite De Brito, Letícia Santos Goes, Luciana de Almeida Menezes, Matheus Porto Cortezi, Mayara Paula Paglione, Vanessa Kelen Nunes, Vitória Maximiana Soares Dos Santos, Yasmin Saliba Maluf, Clara Morato Dias, Patricia Parreira Perin, Estevam Guilherme Lux Hoppe, Adolorata Aparecida Bianco Carvalho

{"title":"Coprological assessment of domestic carnivores in public areas and health education focused on larva migrans in São Paulo State, Brazil.","authors":"Abraão Isaque da Silva, Adrieli Leite Araujo Moura, Beatriz Maia Galetti, Bruna Larocca Vieira, Camila Vitória Dias da Silva, Giovana Almeida Reis, Letícia Leite De Brito, Letícia Santos Goes, Luciana de Almeida Menezes, Matheus Porto Cortezi, Mayara Paula Paglione, Vanessa Kelen Nunes, Vitória Maximiana Soares Dos Santos, Yasmin Saliba Maluf, Clara Morato Dias, Patricia Parreira Perin, Estevam Guilherme Lux Hoppe, Adolorata Aparecida Bianco Carvalho","doi":"10.1590/S1984-29612025071","DOIUrl":"10.1590/S1984-29612025071","url":null,"abstract":"Cutaneous larva migrans (CLM) and visceral larva migrans (VLM) are neglected zoonoses caused by Ancylostoma spp. and Toxocara spp. Although investigations have been conducted in some areas of São Paulo State, Brazil, the presence of these helminth eggs in domestic carnivores' feces from public areas in Jaboticabal (SP) had not previously been studied. This study aimed to detect Ancylostoma spp. and Toxocara spp. eggs in fecal samples collected in public squares using the Willis-Mollay method and to assess the community's knowledge related to public health topics through a structured questionnaire. A total of 88 fecal samples were collected in 32 localities of the city, of which 12.5% (11/88) were positive for eggs of Ancylostoma spp. and none for Toxocara spp. The answers to the questionnaire revealed a direct trend between education level and knowledge of zoonotic risks, indicating that limited knowledge can be associated with a greater exposure to infection. This is the first study to report the presence of Ancylostoma spp. eggs in domestic carnivores' feces in Jaboticabal. The results highlight the risk of CLM and reinforce the need for epidemiological surveillance in dogs and cats, along health education initiatives to promote regular deworming and the diagnostic testing of pets.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e009925"},"PeriodicalIF":1.2,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145726875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mutilation on gill filaments of Piaractus brachypomus (Characiformes: Serrasalmidae) caused by Braga patagonica (Crustacea: Cymothoidae), in the Brazilian Amazon. 巴西亚马逊河流域短尾棘虾鳃丝受巴塔哥尼加（甲壳纲：棘虾科）侵染的危害。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-08 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025068

Marcos Sidney Brito Oliveira, Marcos Tavares-Dias

{"title":"Mutilation on gill filaments of Piaractus brachypomus (Characiformes: Serrasalmidae) caused by Braga patagonica (Crustacea: Cymothoidae), in the Brazilian Amazon.","authors":"Marcos Sidney Brito Oliveira, Marcos Tavares-Dias","doi":"10.1590/S1984-29612025068","DOIUrl":"10.1590/S1984-29612025068","url":null,"abstract":"Fish aquaculture is one of the main food production sectors for the growing global human population and is currently one of the most profitable production activities. However, parasitic infestations have emerged as a significant factor influencing fish aquaculture production, negatively impacting the profitability of this food production activity. This study aimed to report the mutilation of pirapitinga Piaractus brachypomus gill filaments caused by a Cymothoidae parasite. In April 2022, Piaractus brachypomus were examined at a fish farm in Macapá, state of Amapá, Brazil. The host fish were analyzed, and the parasite found was preserved in 70% ethyl alcohol for taxonomic identification. Of the 31 fish examined, one had its gills infested with a crustacean parasite identified as Braga patagonica. A female parasite caused macroscopic mutilation of the filaments on the four right gill arches of P. brachypomus and severe damage at the attachment site. This is the first record of B. patagonica infestation in P. brachypomus. The fish farming water supply allowed the isopod ectoparasite to pass from wild fish to the pond, where it consequently found favorable conditions in the host fish.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e012725"},"PeriodicalIF":1.2,"publicationDate":"2025-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145726845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic characterization of Fasciola hepatica (Linnaeus, 1758) in cattle from Paraná, Brazil. 巴西帕拉纳<e:1>牛肝片形吸虫（Linnaeus, 1758）的遗传特征。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-12-05 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025069

Desiree Vera Pontarolo, Gilberto Augusto Krug, Pedro Mesquita Fonseca, Daniel Angelo Sganzerla Graichen, Marcelo Beltrão Molento

{"title":"Genetic characterization of Fasciola hepatica (Linnaeus, 1758) in cattle from Paraná, Brazil.","authors":"Desiree Vera Pontarolo, Gilberto Augusto Krug, Pedro Mesquita Fonseca, Daniel Angelo Sganzerla Graichen, Marcelo Beltrão Molento","doi":"10.1590/S1984-29612025069","DOIUrl":"10.1590/S1984-29612025069","url":null,"abstract":"Fasciolosis is a foodborne anthropozoonotic disease caused by Fasciola spp. that affects multiple hosts worldwide. Genetic characterization studies have revealed considerable diversity within F. hepatica populations owing to human and animal migration and intermediate snail hosts. Molecular markers such as microsatellites and mitochondrial DNA sequences are useful tools for assessing parasite population dynamics and evolutionary history. In this study, 16 F. hepatica samples were analyzed using cytochrome c oxidase 1 (CO1) and nicotinamide dehydrogenase (NADH) genes. Parasites were collected from the livers of naturally infected cattle originating from 14 municipalities in Paraná, Brazil. Both CO1 and NADH sequences showed high homogeneity, with mean genetic distances <1% (0.0084 and 0.0092, respectively). Network analyses revealed seven CO1 haplotypes and four NADH haplotypes among the new Paraná samples. When compared with reference sequences available in GenBank from other Brazilian states (Rio Grande do Sul and São Paulo), the Paraná samples also displayed <1% genetic divergence. This high level of homogeneity suggests a common and stable origin, with limited gene flow across regions. The genetic information reported here can support the development of targeted interventions, such as vaccines and drugs, aimed at controlling similar F. hepatica genetic variants.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e008325"},"PeriodicalIF":1.2,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145726857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First record of Spirometra spp. eggs in fecal samples from Panthera onca in the Brazilian Pantanal: a One Health approach. 首次在巴西潘塔纳尔地区的黑豹粪便样本中发现螺虫卵：同一健康方法。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-11-28 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025066

Paul Raad, Flávio Paixão de Alencar Junior, Richard de Campos Pacheco, Samuel Felipe Bressan, José Gabriel Gonçalves Lins, Felipe Fornazari

{"title":"First record of Spirometra spp. eggs in fecal samples from Panthera onca in the Brazilian Pantanal: a One Health approach.","authors":"Paul Raad, Flávio Paixão de Alencar Junior, Richard de Campos Pacheco, Samuel Felipe Bressan, José Gabriel Gonçalves Lins, Felipe Fornazari","doi":"10.1590/S1984-29612025066","DOIUrl":"10.1590/S1984-29612025066","url":null,"abstract":"This study examines the presence of gastrointestinal parasites in fecal samples from free-ranging jaguars (Panthera onca) in the Pantanal biome and assesses their potential as bioindicators of environmental health at the human-animal interface using a noninvasive approach. In 2024, ten fresh fecal samples were collected from the ground at Piuval Lodge in the northern Pantanal, state of Mato Grosso, Brazil. All samples were morphologically consistent with large felids, suggesting a high likelihood of originating from jaguars, based on field evidence. Coproparasitological analysis using sedimentation and flotation techniques identified Spirometra spp. eggs, with a positivity rate of 100%. Additionally, 20% of the samples tested positive for Toxocara spp. and one sample was positive for the genus Ancylostoma. This study highlights that jaguars (Panthera onca) are a potential bioindicator of environmental health and a sentinel species in the Pantanal, thus emphasizing the interaction between wildlife, domestic animals and human activities.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e008425"},"PeriodicalIF":1.2,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12704773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145670390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae). 长角血蜱二硫异构酶的鉴定及抑菌作用。

IF 1.2 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-11-17 eCollection Date: 2025-01-01 DOI: 10.1590/S1984-29612025058

Md Samiul Haque, Bumseok Kim, Myung-Jo You

{"title":"Identification and knockdown effect of disulfide isomerase in the Haemaphysalis longicornis (Acari: Ixodidae).","authors":"Md Samiul Haque, Bumseok Kim, Myung-Jo You","doi":"10.1590/S1984-29612025058","DOIUrl":"10.1590/S1984-29612025058","url":null,"abstract":"Tick-borne diseases are a leading cause of death and illness worldwide. Disulfide isomerase (DSI) is an essential protein that helps tick bodies form natural protein structures so they can perform their biological functions after engorgement. In this study, we are exploring the role of DSI and present a potential strategy for tick management by interfering with DSI in ticks. HlDSI cDNA contains 1,119 nucleotides encoding 372 amino acids, and its molecular weight is 93.69 kilodaltons. BLAST analysis showed that the HlDSI protein was 99.10% identical to DSI-like proteins of other parasites. We identified the Jeju strain of Haemaphysalis longicornis and characterized its transcriptional and functional status. Each tick's dsRNA was injected into a female tick and soaked in nymph to further examine its biological functions. RT-PCR and real-time PCR were used to identify and decrease the gene expression of HlDSI RNA interference (RNAi). In adult tick dsRNA-injected groups, HlDSI RNAi significantly impaired tick blood-feeding efficiency and tick viability,and disrupted the molting process in nymphs. According to our analysis, DSI is a significant molecule that is involved in both feeding and reproduction during the tick life cycle,and is therefore a valid target for future tick control strategies.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"34 4","pages":"e009125"},"PeriodicalIF":1.2,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12643237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145558134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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