Revista Brasileira De Parasitologia Veterinaria最新文献

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Eco-epidemiological analysis of Rickettsia parkeri in domestic dogs and Amblyomma ovale ticks in the Atlantic rainforest of Northeast Brazil.
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-12-16 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024077
Michellin Pereira de Albuquerque, Mauricio Claudio Horta, Daniele Rosa Xavier de Melo, Gabriela Akemi Cardoso Gagliardi Takeda, Ana Isabel Arraes-Santos, Thiago Fernandes Martins, Adriano Pinter
{"title":"Eco-epidemiological analysis of Rickettsia parkeri in domestic dogs and Amblyomma ovale ticks in the Atlantic rainforest of Northeast Brazil.","authors":"Michellin Pereira de Albuquerque, Mauricio Claudio Horta, Daniele Rosa Xavier de Melo, Gabriela Akemi Cardoso Gagliardi Takeda, Ana Isabel Arraes-Santos, Thiago Fernandes Martins, Adriano Pinter","doi":"10.1590/S1984-29612024077","DOIUrl":"https://doi.org/10.1590/S1984-29612024077","url":null,"abstract":"<p><p>In Brazil, spotted fever (SF) is caused by two species of Rickettsia, both of which are transmitted by Amblyomma ticks: Rickettsia rickettsii, which results in severe and often fatal cases, and Rickettsia parkeri, which causes a mild illness. This study focused on R. parkeri in Amblyomma ovale ticks from the Maciço de Baturité region, Ceará, Northeast Brazil, an area endemic for SF with mild symptoms. We examined 60 domestic dogs with access to the forest for ticks and Rickettsia seroprevalence. A landscape analysis was conducted in all forest patches within 2-10 km from the main forest edge. In total, 125 A. ovale ticks were collected from 30 dogs (50%). DNA from 65 ticks was tested using genus-specific Rickettsia primers. Three (4.6%) tick specimens tested positive for R. parkeri while the Rickettsia seroprevalence among the dogs was 55% (33/60). A probable occurrence of Rickettsia transmission was observed in the fragmented Atlantic rainforest, which has 1,019 ha of preserved land and 50.6 km of perimeter border. The land's characteristics allow for semi-domiciled dogs to access forest fragments, where A. ovale ticks are commonly present. Infected ticks may parasitize the dogs, which then transport the ticks into homes, potentially transmitting SF-causing bacteria to humans.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e012524"},"PeriodicalIF":1.3,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new Ceratomyxa (Cnidaria: Myxosporea) infecting the ornamental fish species Pterophyllum scalare from the Amazon Region, Brazil.
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-12-13 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024075
Rafaela Franco de Araújo, Abthyllane Amaral de Carvalho, Roger Leomar da Silva Ferreira, Saturo Cardoso Morais, Luize Cristine Pantoja Dos Reis, Pedro Lucas Dos Santos de Oliveira, Marcela Nunes Videira, Aldi Feiden
{"title":"A new Ceratomyxa (Cnidaria: Myxosporea) infecting the ornamental fish species Pterophyllum scalare from the Amazon Region, Brazil.","authors":"Rafaela Franco de Araújo, Abthyllane Amaral de Carvalho, Roger Leomar da Silva Ferreira, Saturo Cardoso Morais, Luize Cristine Pantoja Dos Reis, Pedro Lucas Dos Santos de Oliveira, Marcela Nunes Videira, Aldi Feiden","doi":"10.1590/S1984-29612024075","DOIUrl":"https://doi.org/10.1590/S1984-29612024075","url":null,"abstract":"<p><p>A new parasite of the Class Myxozoa is described in the gallbladder of the ornamental angelfish Pterophyllum scalare, in two municipalities in the state of Amapá, Brazil, based on morphological, morphometric and phylogenetic descriptions. From October 2022 to August 2024 fifty-five angelfish specimens were sampled in Macapá (n=10) and Tartarugalzinho (n=45). Slightly arched mixospores were observed by light microscopy and had characteristics consistent with those of the genus Ceratomyxa. These obtained an average length of 1.6 ± 0.2 µm and 11.5 ± 1.1 µm in thickness. The polar capsules were subspherical and 0.7 ± 0.1 µm long and 0.6 ± 0.1 µm wide, with 3 to 4 turns of the polar filament. Phylogenetic analysis showed that the new species is grouped in the family Ceratomyxidae, in addition to being positioned in the same subclade of freshwater ceratomyxids from the Brazilian Amazon, demonstrating that this species shares a common ancestor with its close relatives, based on geographic affinity. Ceratomyxa tavariensis n. sp. is the first species of the class Myxozoa described infecting angelfish in Brazil, and the thirteenth species of Ceratomyxa described in the country.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e018324"},"PeriodicalIF":1.3,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parasite infestations and infections of non-traditional pets and wild mammals: diagnosis and treatment.
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-12-13 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024074
Raphael Vieira Ramos, Tiago Manuel Fernandes Mendes, Estevam Lux Hoppe, Darci Moraes Barros-Battesti, Marlene Tiduko Ueta, Silmara Marques Allegretti
{"title":"Parasite infestations and infections of non-traditional pets and wild mammals: diagnosis and treatment.","authors":"Raphael Vieira Ramos, Tiago Manuel Fernandes Mendes, Estevam Lux Hoppe, Darci Moraes Barros-Battesti, Marlene Tiduko Ueta, Silmara Marques Allegretti","doi":"10.1590/S1984-29612024074","DOIUrl":"https://doi.org/10.1590/S1984-29612024074","url":null,"abstract":"<p><p>Little is known about parasites in wild mammals kept as pets. For this study, fecal and skin/fur samples and ectoparasites from 55 wild and pet mammals attended at a veterinary clinic were evaluated. Opossums (Didelphis albiventris and Didelphis aurita) were parasitized by helminths (Aspidodera sp., Cruzia tentaculata, Trichuris sp., Turgida turgida and Acanthocephala gen. sp.), screw-worm fly larvae (Cochliomyia hominivorax), ticks (Amblyomma dubitatum and Amblyomma sculptum) and fleas (Ctenocephalides felis). Hedgehogs (Atelerix albiventris) were parasitized by mites (Caparinia tripilis), capybaras (Hydrochoerus hydrochaeris) by ticks (A. dubitatum and A. sculptum), a ferret (Mustela putorius furo) by fleas (C. felis), an orange-spined hairy dwarf porcupine (Sphiggurus villosus) by screw-worm fly larvae (C. hominivorax) and another for ticks (Amblyomma longirostre). Overall, mites were found only in pet animals and helminths were found only in wild animals. Infestation by Caparinia tripilis was only found in animals with concomitant illness or stress. Cruzia tentaculata actively exited its opossum host by passing through the animal's anus as its clinical condition worsened.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e017324"},"PeriodicalIF":1.3,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142856239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Enzootic transmission of Leishmania spp. in gallery forests of the Brazilian Cerrado.
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-12-06 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024073
Aline Rapello, Andrey José de Andrade, Nadjar Nitz, Thaís Tâmara Castro Minuzzi-Sousa, Tamires Emanuele Vital, Tauana de Sousa Ferreira, Douglas de Almeida Rocha, Marcos Takashi Obara, Renata Velôzo Timbó, Jônatas Barbosa Cavalcante Ferreira, Rodrigo Gurgel-Gonçalves
{"title":"Enzootic transmission of Leishmania spp. in gallery forests of the Brazilian Cerrado.","authors":"Aline Rapello, Andrey José de Andrade, Nadjar Nitz, Thaís Tâmara Castro Minuzzi-Sousa, Tamires Emanuele Vital, Tauana de Sousa Ferreira, Douglas de Almeida Rocha, Marcos Takashi Obara, Renata Velôzo Timbó, Jônatas Barbosa Cavalcante Ferreira, Rodrigo Gurgel-Gonçalves","doi":"10.1590/S1984-29612024073","DOIUrl":"https://doi.org/10.1590/S1984-29612024073","url":null,"abstract":"<p><p>Gallery forests harbor mammals and sand flies that may be involved in the transmission of Leishmania spp. parasites. Characterizing the enzootic cycles of Leishmania spp. is essential for understanding its transmission dynamics. We analyzed the presence of Leishmania spp. in mammals and sand flies in gallery forests during the dry season in the Cerrado. Four gallery forests were investigated in May and September 2014. Our capture effort included 1,280 HP trap-nights, 16 Shannon trap-nights for sand flies, and 5,120 trap-nights for mammals. After identifying the mammalian and sand fly species, SSU rRNA and ITS-1 polymerase chain reaction (PCR) were used to detect Leishmania spp. A total of 1,209 sand flies belonging to 13 species were captured, mainly Bichromomyia flaviscutellata. Leishmania spp. DNA was not detected in the analyzed sand fly females. PCR analysis of 153 mammals revealed Leishmania spp. in 20 samples (13%) in May (early dry season), when the infection rate was 31% in one gallery forest. The host species were Rhipidomys macrurus, Gracilinanus agilis, and Didelphis albiventris. We observed a low frequency of mammals infected with Leishmania spp., which was not detected in sand flies. Our results indicate that Leishmania spp. infection is higher in mammals during the early dry season in Cerrado gallery forests.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e011624"},"PeriodicalIF":1.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence of Leishmania spp. DNA and specific antibodies in dogs from Acre State, Rio Branco, Brazil.
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-12-02 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024072
Gleice Kelly Carvalho Bento, Leticia Gomes Zanfagnini, Marcia Dalastra Laurenti, Thayse Yumie Tomokane, Vania Lucia Ribeiro da Matta, Soraia Figueiredo Souza, Acácio Duarte Pacheco
{"title":"Occurrence of Leishmania spp. DNA and specific antibodies in dogs from Acre State, Rio Branco, Brazil.","authors":"Gleice Kelly Carvalho Bento, Leticia Gomes Zanfagnini, Marcia Dalastra Laurenti, Thayse Yumie Tomokane, Vania Lucia Ribeiro da Matta, Soraia Figueiredo Souza, Acácio Duarte Pacheco","doi":"10.1590/S1984-29612024072","DOIUrl":"10.1590/S1984-29612024072","url":null,"abstract":"<p><p>Canine leishmaniasis is a parasitic disease whose agents are transmitted through the bites of infected phlebotomine sand flies. This disease is endemic in tropical and subtropical regions, including Brazil. However, information on its prevalence in dogs in some Brazilian states remains limited. This study aimed to assess the seroprevalence of canine leishmaniasis in Rio Branco, Brazil. Blood samples were collected from 375 dogs aged > 6 months. Two distinct serological methods, dual path platform test (DPP) and enzyme linked immunosorbent assay (ELISA), were used to investigate the occurrence of anti-Leishmania spp. antibodies. The results showed a seroprevalence of 38.1%, indicating that the disease occurred in this region. Blood samples considered positive in at least one of the serological methods were subjected to conventional polymerase chain reaction (PCR), which confirmed the presence of infection in 28.3% (106/375) of the total samples. This is the first study to provide detailed information on the seroprevalence of canine leishmaniasis in dogs in Rio Branco, highlighting the importance of disease surveillance and control. Effective actions, such as education campaigns on sand fly prevention and control measures, are necessary to reduce the occurrence of canine and human leishmaniasis in cities.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e010824"},"PeriodicalIF":1.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anti-Pterobothrium heteracanthum (Trypanorhyncha: Pterobothriidae) IgG in human serum samples. 人类血清样本中的抗异翅虫(Trypanorhyncha: Pterobothriidae)IgG。
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-11-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024071
Maurício Afonso Verícimo, Israel Figueiredo Júnior, Sérgio Carmona de São Clemente, Michelle Cristie Gonçalves da Fonseca, Marcelo Knoff, Danuza Pinheiro Bastos Garcia de Mattos
{"title":"Anti-Pterobothrium heteracanthum (Trypanorhyncha: Pterobothriidae) IgG in human serum samples.","authors":"Maurício Afonso Verícimo, Israel Figueiredo Júnior, Sérgio Carmona de São Clemente, Michelle Cristie Gonçalves da Fonseca, Marcelo Knoff, Danuza Pinheiro Bastos Garcia de Mattos","doi":"10.1590/S1984-29612024071","DOIUrl":"10.1590/S1984-29612024071","url":null,"abstract":"<p><p>Some fish parasites can cause a variety of symptoms in humans, including allergies. This was a cross-sectional study based on interviews, serum analysis by ELISA for anti-Pterobothrium heteracanthum IgG and a statistical evaluation. Four individuals were seroreactive (6.25%), with no association with fish handling (p = 1.000) or with ingestion more than twice a week (p = 0.232). There was a significant association (p = 0.032) between reactivity and the absence of allergy symptoms. Seroreactivity against P. heteracanthum in humans was detected, but was not associated with the amount of fish ingested, handled, or with allergic complaints.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e014724"},"PeriodicalIF":1.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614087/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection and quantification of Dioctophyme renale eggs in dog urine after nephrectomy. 肾切除术后狗尿液中肾脏蝶形虫卵的检测和定量。
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-11-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024067
Soliane Carra Perera, Maria Antonieta Machado Pereira da Silva, Gabriela de Almeida Capella, Natália Berne Pinheiro, Maria Elisabeth Aires Berne, Josaine Cristina da Silva Rappeti, Fabrício de Vargas Arigony Braga, Marlete Brum Cleff
{"title":"Detection and quantification of Dioctophyme renale eggs in dog urine after nephrectomy.","authors":"Soliane Carra Perera, Maria Antonieta Machado Pereira da Silva, Gabriela de Almeida Capella, Natália Berne Pinheiro, Maria Elisabeth Aires Berne, Josaine Cristina da Silva Rappeti, Fabrício de Vargas Arigony Braga, Marlete Brum Cleff","doi":"10.1590/S1984-29612024067","DOIUrl":"10.1590/S1984-29612024067","url":null,"abstract":"<p><p>Dioctophyme renale is a zoonotic nematode that parasitizes mainly right kidney of domestic and wild canines, and can affect humans, and its eggs are eliminated in urine. The duration of egg dissemination after surgical treatment is unknown. Therefore, the objective of this study was to identify, quantify, and verify the duration of the elimination of D. renale eggs in dog urine after the surgical removal of nematodes. The study involved 15 dogs in which female specimens of D. renale were detected in kidneys. Urine samples, preferably first-morning urine samples, were collected before and for the first ten days after nephrectomy. For egg quantification, 40 µL samples of urinary sediment were analyzed in triplicate. In laboratory analyses, between 900 and > 6,000 eggs/urine sample were detected in 86.7% of the dogs prior to surgery, and in 40% of the dogs on postoperative day 1. Of the 15 dogs evaluated, 14 (93.3%) eliminated D. renale eggs on each of the first ten postoperative days. Egg elimination peaked on postoperative day 1. Our results indicate that dogs can continue to be sources of D. renale infection even after the helminths have been removed from a parasitized kidney, underscoring the novelty of these findings and their importance for the One Health approach.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e016224"},"PeriodicalIF":1.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614090/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gastrointestinal Platyhelminths of Free-Living Cuniculus paca (Linnaeus, 1766) in the Western Amazon. 亚马逊河西部自由生活的 Cuniculus paca(林奈,1766 年)的胃肠道板虫。
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-11-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024069
Victor Silva Vasconcelos, Maria Isabel Afonso da Silva, Rodrigo Lima do Nascimento, Matheus Nascimento Oliveira, Rodrigo Cacique Rocha, Manuela Jucá Correia, Cledson Kauã Araújo Silva, Wendell Nogueira Dias, Felipe Bisaggio Pereira, Iago de Sá Moraes, Dirceu Guilherme de Souza Ramos, Tiago Lucena da Silva
{"title":"Gastrointestinal Platyhelminths of Free-Living Cuniculus paca (Linnaeus, 1766) in the Western Amazon.","authors":"Victor Silva Vasconcelos, Maria Isabel Afonso da Silva, Rodrigo Lima do Nascimento, Matheus Nascimento Oliveira, Rodrigo Cacique Rocha, Manuela Jucá Correia, Cledson Kauã Araújo Silva, Wendell Nogueira Dias, Felipe Bisaggio Pereira, Iago de Sá Moraes, Dirceu Guilherme de Souza Ramos, Tiago Lucena da Silva","doi":"10.1590/S1984-29612024069","DOIUrl":"10.1590/S1984-29612024069","url":null,"abstract":"<p><p>Studies on Brazil's helminthological fauna began in the early 20th century, and since then several zoologists from different parts of the country have focused on parasitology. Recent studies have advanced our understanding of helminthological biodiversity in the Amazon region, especially in fish, which is essential for the Amazonian economy. This study aimed to inventory the platyhelminth fauna Cuniculus paca (paca). The viscera of 30 pacas were analyzed, and 60 specimens of platyhelminths were identified, including 52 cestodes and 8 trematodes. Cestodes belonging to the family Davaneidae, identified as Raillietina spp., with an occurrence of 26.67% (n = 8), mean abundance 1.83 and mean intensity of 6.5, were found in the small intestine. The trematodes found in the large intestine were identified as Stichorchis spp., with an occurrence of 6.6% (n = 2), mean abudance 0.32 and a mean intensity of four. To the genus Raillietina, we provide new insights into the helminth fauna of this game rodent in the Western Amazon. The discovery of a new site of occurrence for the genus Stichorchis highlights the gap in knowledge regarding the parasitic diversity of consumed animals in the extreme western Amazon region, emphasizing the need for more in-depth scientific investigations in this area.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e015724"},"PeriodicalIF":1.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614092/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization and workflow of in vitro culture of adult Fasciola hepatica. 肝吸虫成虫体外培养的优化和工作流程。
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-11-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024064
Cesar Burga-Cisterna, Edith Málaga, Enrique Serrano-Martínez, Giovana Livia-Córdova, Ramón Antezana, Américo Castro Luna, Monica Pajuelo
{"title":"Optimization and workflow of in vitro culture of adult Fasciola hepatica.","authors":"Cesar Burga-Cisterna, Edith Málaga, Enrique Serrano-Martínez, Giovana Livia-Córdova, Ramón Antezana, Américo Castro Luna, Monica Pajuelo","doi":"10.1590/S1984-29612024064","DOIUrl":"10.1590/S1984-29612024064","url":null,"abstract":"<p><p>The aim of this study was to evaluate different transportation and incubation conditions to test the viability of adult Fasciola hepatica in order to propose a new cultivation workflow. The adult stage of F. hepatica was obtained from naturally infected cattle at a local slaughterhouse in Lima, Peru. Different transport and incubation conditions of F. hepatica were tested, evaluating its viability through a motility scale. DMEM and RPMI 1640 media presented better transport conditions compared to Hedon-Fleig and PBS media (p < 0.001), maintaining the flukes at 37°C. Also, DMEM and RPMI-1640 media presented better incubation conditions compared to Hedon-fleig (p < 0.001). A minimum of 3 ml of medium per fluke was required to maintain best viability (p < 0.001) and no differences in viability were found between the different types of culture plates (p > 0.05). In addition, we found that incubation with DMSO (dimethyl sulfoxide) at concentrations greater than 0.5% v/v for 48 hours generates toxicity (p < 0.001). In conclusion, RPMI 1640 and DMEN media presented better transport and in vitro cultivation conditions for F. hepatica, using DMSO at concentrations lower than 0.5% v/v.</p>","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 4","pages":"e014524"},"PeriodicalIF":1.3,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142741093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
First record of Haematopinus tuberculatus (Burmeister, 1839) (Psocodea: Anoplura: Haematopinidae) parasitizing buffalo (Bubalus bubalis) in the state of Amazonas, Brazil. 巴西亚马孙州首次记录到 Haematopinus tuberculatus (Burmeister, 1839) (Psocodea: Anoplura: Haematopinidae)寄生于水牛(Bubalus bubalis)。
IF 1.3 4区 农林科学
Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-11-22 eCollection Date: 2024-01-01 DOI: 10.1590/S1984-29612024070
José Vicente Ferreira Neto, Marcelo Cutrim Moreira de Castro, Alexandre Levi Monteiro Santana, Gabriel Moreira Valença, André de Abreu Rangel Aguirre, Ahana Maitra, Felipe Arley Costa Pessoa
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