Revista Brasileira De Parasitologia Veterinaria最新文献_第10页

Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching. 从寄生植物 Arceuthobium vaginatum 中提取的生物活性化合物可抑制血吸虫卵孵化。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-22 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612024004

María Mitsi Nalleli Becerril-Gil, Julieta Gertrudis Estrada-Flores, Manases González-Cortazar, Alejandro Zamilpa, Ángel Rolando Endara-Agramont, Pedro Mendoza-de Gives, María Eugenia López-Arellano, Agustín Olmedo-Juárez

{"title":"Bioactive compounds from the parasitic plant Arceuthobium vaginatum inhibit Haemonchus contortus egg hatching.","authors":"María Mitsi Nalleli Becerril-Gil, Julieta Gertrudis Estrada-Flores, Manases González-Cortazar, Alejandro Zamilpa, Ángel Rolando Endara-Agramont, Pedro Mendoza-de Gives, María Eugenia López-Arellano, Agustín Olmedo-Juárez","doi":"10.1590/S1984-29612024004","DOIUrl":"10.1590/S1984-29612024004","url":null,"abstract":"The aim of this study was to assess the in vitro ovicidal activity of an ethyl acetate extract from Arceuthobium vaginatum (EtOAc-E) and their subfractions (AvR5-AvR14) against Haemonchus contortus using the egg hatching inhibition (EHI) test. The EtOAc-E and subfractions were tested at 0.12-2.00 and at 0.015-2.0 mg/mL, respectively. Distilled water and methanol (2%) were used as negative controls and Thiabendazole (0.10 mg/mL) as a positive control. Treatments with a dependent effect on concentration were subjected to regression analysis to determine the effective concentrations (EC50 and EC90). The major secondary compounds present in the extract and subfractions were identified by high performance liquid chromatography (HPLC). The EtOAc-E and AvR9 exhibited the best ovicidal effect recording 97.5 and 100% of EHI at 0.25 mg/mL, respectively. The EtOAc-E and AvR9 displayed an EC50= 0.12 and 0.08 mg/mL, respectively. The HPLC analysis in the EtOAc-E and bioactive fractions indicated the presence of a polyphenol, glycosylated flavanones, quercetin glucoside, cinnamates, coumarin, cinnamic acid derivative, ferulic acid, coumarate, naringenin, protocatechuic acid and naringin. Results demonstrated that A. vaginatum extract and fraction is able to inhibit the egg hatch process of H. contortus and could be a viable option for the control of small ruminant haemonchosis.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 1","pages":"e013223"},"PeriodicalIF":1.3,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10878698/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138832545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noteworthy records of the ticks Ornithodoros rostratus and Amblyomma sculptum parasitizing Pteronura brasiliensis in the central-western region of Brazil, with pathogen investigation notes. 巴西中西部地区值得注意的寄生于巴西翼龙（Pteronura brasiliensis）的蜱Ornithodoros rostratus和Amblyomma sculptum的记录，以及病原体调查说明。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-22 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612024003

Darci Moraes Barros-Battesti, Marcos Rogério André, Estevam Guilherme Lux Hoppe, Gustavo Seron, Ricardo Bassini-Silva, Ana Claúdia Calchi, Lívia Oliveira Andrade, Patrícia Parreira Perin, Thiago Fernandes Martins, Ana Carolina Castro-Santiago, Caroline Leuchtenberger, Samara Almeida, Nathalie Foerster, Mariana Furtado, Fernando de Castro Jacinavicius

{"title":"Noteworthy records of the ticks Ornithodoros rostratus and Amblyomma sculptum parasitizing Pteronura brasiliensis in the central-western region of Brazil, with pathogen investigation notes.","authors":"Darci Moraes Barros-Battesti, Marcos Rogério André, Estevam Guilherme Lux Hoppe, Gustavo Seron, Ricardo Bassini-Silva, Ana Claúdia Calchi, Lívia Oliveira Andrade, Patrícia Parreira Perin, Thiago Fernandes Martins, Ana Carolina Castro-Santiago, Caroline Leuchtenberger, Samara Almeida, Nathalie Foerster, Mariana Furtado, Fernando de Castro Jacinavicius","doi":"10.1590/S1984-29612024003","DOIUrl":"10.1590/S1984-29612024003","url":null,"abstract":"A male of Pteronura brasiliensis (Carnivora: Mustelidae) was found dead on the banks of the Rio Negro, in the Pantanal wetlands of Mato Grosso do Sul state, Aquidauana municipality. Two ticks found attached to its skin were morphologically identified as a second-instar nymph of Ornithodoros rostratus (Argasidae) and a male of Amblyomma sculptum (Ixodidae). In order to complement the morphological identification, these tick specimens were subjected to DNA extraction, and tested using PCR assays to confirm the molecular identity the specimens. Also, the tick DNA samples were tested and were negative in the PCR assays for all the pathogens tested. We also examined 30 batches, consisting of 174 individuals of O. rostratus deposited in the Acari Collection of the Butantan Institute, and we found material from four Brazilian states, including one batch containing 2 males and 2 females from Aquidauana, of Mato Grosso do Sul state, collected from the soil. This was therefore the first record of O. rostratus parasitizing P. brasiliensis and the first locality record (Aquidauana). Likewise, A. sculptum is commonly found in the Pantanal and is reported here for the second time parasitizing the giant otter, which is a host little studied regarding the ectoparasites.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 1","pages":"e014523"},"PeriodicalIF":1.3,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10878696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138832547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Essential oil of Piper hispidum (Piperaceae) has efficacy against monogeneans, and effects on hematology and gill histology of Colossoma macropomum. 胡椒科植物胡椒的精油具有抗单胞菌的功效，并对大疣梭子蟹的血液学和鳃组织学有影响。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-11 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612024001

Carliane Maria Guimarães Alves, Raimundo Rosemiro de Jesus Baia, Vitor Araújo Farias, Matheus Araújo Farias, Fernanda Layza Souza de Souza, Marcela Nunes Videira, Francisco Célio Maia Chagas, Eliane Tie Oba Yoshioka, Marcos Tavares-Dias

{"title":"Essential oil of Piper hispidum (Piperaceae) has efficacy against monogeneans, and effects on hematology and gill histology of Colossoma macropomum.","authors":"Carliane Maria Guimarães Alves, Raimundo Rosemiro de Jesus Baia, Vitor Araújo Farias, Matheus Araújo Farias, Fernanda Layza Souza de Souza, Marcela Nunes Videira, Francisco Célio Maia Chagas, Eliane Tie Oba Yoshioka, Marcos Tavares-Dias","doi":"10.1590/S1984-29612024001","DOIUrl":"10.1590/S1984-29612024001","url":null,"abstract":"This study investigated for the first time the effectiveness of therapeutic baths with essential oil (EO) of Piper hispidum against monogeneans Anacanthorus spathulatus, Notozothecium janauachensis, Mymarothecium boegeri and Linguadactyloides brinkmanni from the gills of Colossoma macropomum, as well as the hematological and histological effects on this fish. In therapeutic baths, 100 mg/L of P. hispidum essential oil and two control groups (water from the culture tank and water from the culture tank with 70% alcohol) were exposed for 1 h/day, with intervals of 48 hours for 3 days, and three replicates each were used. Therapeutic baths with 100 mg/L of P. hispidum essential oil had an efficacy of 78.6% against monogeneans. The toxicity of this essential oil was low, since there were a few physiological and histopathological changes that did not compromise the functioning of the gills of the fish. Therefore, 100 mg/L of P. hispidum essential oil was effective for controlling monogeneans in C. macropomum, when short therapeutic baths were used without compromising the health of the exposed fish.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"33 1","pages":"e014723"},"PeriodicalIF":1.3,"publicationDate":"2023-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10782507/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of anti-Leishmania spp. antibodies in poultry from central region of Rio Grande do Sul, Brazil. 在巴西南里奥格兰德州中部地区的家禽中检测抗利什曼原虫抗体。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-08 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023077

Maurício Tatto, Fagner D'ambroso Fernandes, Eliesse Pereira Costa, Fabio Yuji Shibuya, Luiza Isaia de Freitas, Vanessa Osmari, Isac Junior Roman, Patrícia Bräunig, Fernanda Silveira Flores Vogel, Sônia de Avila Botton, Luis Antônio Sangioni

{"title":"Detection of anti-Leishmania spp. antibodies in poultry from central region of Rio Grande do Sul, Brazil.","authors":"Maurício Tatto, Fagner D'ambroso Fernandes, Eliesse Pereira Costa, Fabio Yuji Shibuya, Luiza Isaia de Freitas, Vanessa Osmari, Isac Junior Roman, Patrícia Bräunig, Fernanda Silveira Flores Vogel, Sônia de Avila Botton, Luis Antônio Sangioni","doi":"10.1590/S1984-29612023077","DOIUrl":"10.1590/S1984-29612023077","url":null,"abstract":"Domestic birds such as Gallus gallus, Meleagris gallopavo, Anser anser and Numida meleagris are widely distributed throughout the world and maintain contact with humans and other animal species considered reservoirs of both Visceral Leishmaniasis (VL) and American Tegumentary Leishmaniasis (ATL), including dogs and cats; wild canids, marsupials; and synanthropic animals such as rodents and chiroptera. Therefore, this study aimed to detect the presence of anti-Leishmania spp. antibodies in birds from a rural area of the municipality of Santa Maria, southern Brazil. From May to December 2022, 262 blood samples were collected from 244 chickens, 8 turkeys, 7 guinea fowl and 3 geese, distributed in 27 rural properties in 6 districts. All the sites visited presented positive birds for the presence of Leishmania spp. Thus, it is inferred that, contact with this protozoan can induce the production of antibodies, suggesting that these animals can be used as sentinels for the circulation of this agent. In addition, the blood of these animals is a preferred food source for insects of the subfamily Phlebotominae, which can be used them as bioindicators of the presence of these phlebotomes.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e007723"},"PeriodicalIF":1.3,"publicationDate":"2023-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10750985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138809814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seroprevalence and coprological prevalence of liver fluke Fasciola hepatica in cattle and sheep from Santander department, Colombia. 哥伦比亚桑坦德省牛羊肝脏吸虫肝片吸虫血清阳性率和生殖道流行率。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023071

Nelson Uribe Delgado, Andrés Esteban Pereira, Ruth Aralí Martínez, Angel Alberto Florez Muñoz, Juan Carlos Pinilla

{"title":"Seroprevalence and coprological prevalence of liver fluke Fasciola hepatica in cattle and sheep from Santander department, Colombia.","authors":"Nelson Uribe Delgado, Andrés Esteban Pereira, Ruth Aralí Martínez, Angel Alberto Florez Muñoz, Juan Carlos Pinilla","doi":"10.1590/S1984-29612023071","DOIUrl":"10.1590/S1984-29612023071","url":null,"abstract":"Fasciola hepatica is a parasite with a worldwide distribution that affects several mammals, including humans, and is considered a public health problem. Therefore, the aim of this study was to determine the prevalence of Fasciola hepatica in humans, cattle and sheep, as well as to evaluate factors associated with the prevalence. A total of 185 serum samples from sheep, 290 from cattle, and 114 from humans were collected and processed using an in-house developed ELISA to detect IgG antibodies against F. hepatica. Additionally, 185 stool samples from sheep and 290 from cattle were examined using a Dennis sedimentation technique. Risk factors were analyzed using epidemiological surveys. The overall seroprevalence was 46.5% (86/185) in sheep, 32.5% (94/289) in cattle, and no humans tested positive for the infection. The coprological prevalence was 47.7% (86/180) in sheep and 33.7% (98/290) in cattle. Female gender and cattle living with alternate grazing management showed 2.5 and 6.5 times higher probability of infection, respectively. Bovines coexisting with sheep exhibited a higher risk of infection (odds ratio [OR]=4.3) compared to those without sheep. We concluded that F. hepatica in cattle and sheep has an endemic behavior, and therefore represents a problem of public health for rural communities.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e009923"},"PeriodicalIF":1.3,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10704862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity of helminths with zoonotic potential and molecular characterization of Toxocara canis infecting domestic dogs from locations of Amazon and Atlantic Forest Brazilian biomes. 具有人畜共患潜力的蠕虫多样性及巴西亚马逊和大西洋森林生物群落中感染家养狗的犬弓形虫的分子特征

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023078

Tuan Pedro Dias-Correia, Leandro Batista das Neves, Fernanda Bittencourt-Oliveira, Gabriella Cristina Balzana Giglio, Thiago Cordeiro Pereira, Fernanda Barbosa de Almeida, Rosângela Rodrigues-Silva

{"title":"Diversity of helminths with zoonotic potential and molecular characterization of Toxocara canis infecting domestic dogs from locations of Amazon and Atlantic Forest Brazilian biomes.","authors":"Tuan Pedro Dias-Correia, Leandro Batista das Neves, Fernanda Bittencourt-Oliveira, Gabriella Cristina Balzana Giglio, Thiago Cordeiro Pereira, Fernanda Barbosa de Almeida, Rosângela Rodrigues-Silva","doi":"10.1590/S1984-29612023078","DOIUrl":"10.1590/S1984-29612023078","url":null,"abstract":"The coproparasitological examination of dogs (n=278) from two Brazilian biomes (Amazon [AZ] and Atlantic Forest [AF]) by centrifugal flotation demonstrated positivity values of 54.2% (AF) and 48.5% (AZ). The most prevalent parasites in AF were hookworms (81.0% - 47/58), Toxocara sp. (17.3% - 10/58) and Trichuris vulpis (12.1% - 7/58); while in AZ they were hookworms (86.7% - 72/83), Toxocara sp. (18.1% - 15/83), Dipylidium caninum (13.3% - 11/83) and T. vulpis (10.8% - 9/83). PCR was performed using the partial mitochondrial genes cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase 1 (pnad1) in 25 fecal samples positive for Toxocara sp. eggs and found one sample positive for pcox1 and six positives for pnad1. The sequencing of these samples was unsuccessful due to the difficulties inherent in copro-PCR+sequencing. The sequencing of 14 samples of T. canis adult helminths retrieved 11 sequences of 414 bp for pcox1 and nine sequences of 358 bp for pnad1. The phylogenetic trees of these sequences confirmed the species T. canis. Intraspecific genetic variation was only observed for pnad1. This is the second study involving molecular analysis of T. canis in dogs from Brazil and adds new information through the use of pnad1.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e012723"},"PeriodicalIF":1.3,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10704884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization and epidemiology of Toxoplasma gondii isolates from free-range chickens in the southwest region of Goiás: new genotypes. Goiás西南地区散养鸡刚地弓形虫分离株的分子特征和流行病学:新基因型。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023069

Natália Domann, Stéfanne Rodrigues Rezende, Amanda Cristina Corrêa Fleury, Isa Marianny Ferreira Nascimento Barbosa, Isabella da Costa Ribeiro, Júlia Batista Dornelas, Tainá Francisca Cardoso de Oliveira, Vanessa Oliveira Lopes de Moura, Heloísa Ribeiro Storchilo, Ana Maria de Castro, João Luís Garcia, Ludimila Paula Vaz Cardoso, Hanstter Hallison Alves Rezende

{"title":"Molecular characterization and epidemiology of Toxoplasma gondii isolates from free-range chickens in the southwest region of Goiás: new genotypes.","authors":"Natália Domann, Stéfanne Rodrigues Rezende, Amanda Cristina Corrêa Fleury, Isa Marianny Ferreira Nascimento Barbosa, Isabella da Costa Ribeiro, Júlia Batista Dornelas, Tainá Francisca Cardoso de Oliveira, Vanessa Oliveira Lopes de Moura, Heloísa Ribeiro Storchilo, Ana Maria de Castro, João Luís Garcia, Ludimila Paula Vaz Cardoso, Hanstter Hallison Alves Rezende","doi":"10.1590/S1984-29612023069","DOIUrl":"10.1590/S1984-29612023069","url":null,"abstract":"The purpose of this study was to isolate Toxoplasma gondii from tissues of free-range chickens in the southwestern region of Goiás, to detect and molecularly characterize the genetic material of the parasite, and to determine the seroprevalence of the protozoan parasite in these animals. A seroprevalence of T. gondii antibodies of 76% (19/25) was found among the chickens, while genetic material from their tissues was detected in 56% (14/25). A total of 14 isolates was obtained in the bioassay, ten of which were considered acute, eight were considered isolates of high virulence lethal to mice, and four of low virulence, considered non-lethal but with the ability to chronify the infection. Seven of the ten isolates showed significant morphometric differences from the RH strain, in terms of nucleus-complex-apical distance, length and width. Genotyping of the acute isolates was performed by RFLP-PCR, using 11 genetic markers: SAG1, SAG2 (3'SAG2 and 5'SAG2), alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and APICO. The results were compared and classified according to the genotypes listed on the ToxoDB Platform, where different profiles were observed indicating the presence of two known genotypes (#7 and #63) and five new genotypes (NEW 3, NEW4, NEW5, NEW6, NEW 7). The results showed high seroprevalence, isolation rate, molecular detection and genotypic variations of T. gondii in free-range chickens in the southwestern region of Goiás.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e009823"},"PeriodicalIF":1.3,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10704866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analyses of Chilomastix and Retortamonas species using in vitro excysted flagellates. 利用离体鞭毛虫对赤腹虫和反刍单胞菌进行系统发育分析。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023070

Jun Suzuki, Sanjib Kumar Sardar, Ajanta Ghosal, Naoko Yoshida, Hanako Kurai, Yudai Alex Takahashi, Yumiko Saito-Nakano, Sandipan Ganguly, Seiki Kobayashi

{"title":"Phylogenetic analyses of Chilomastix and Retortamonas species using in vitro excysted flagellates.","authors":"Jun Suzuki, Sanjib Kumar Sardar, Ajanta Ghosal, Naoko Yoshida, Hanako Kurai, Yudai Alex Takahashi, Yumiko Saito-Nakano, Sandipan Ganguly, Seiki Kobayashi","doi":"10.1590/S1984-29612023070","DOIUrl":"10.1590/S1984-29612023070","url":null,"abstract":"In vitro excystation of cysts of microscopically identified Chilomastix mesnili and Retortamonas sp. isolated from Japanese macaques and Retortamonas sp. isolated from small Indian mongooses could be induced using an established protocol for Giardia intestinalis and subsequently by culturing with H2S-rich Robinson's medium supplemented with Desulfovibrio desulfuricans. Excystation usually began 2 h after incubation in Robinson's medium. DNA was isolated from excysted flagellates after 4 h of incubation or from cultured excysted flagellates. Phylogenetic analysis based on their 18S rRNA genes revealed that two isolates of C. mesnili from Japanese macaques belonged to the same cluster as a C. mesnili isolate from humans, whereas a mammalian Retortamonas sp. isolate from a small Indian mongoose belonged to the same cluster as that of an amphibian Retortamonas spp. isolate from a 'poison arrow frog' [sequence identity to AF439347 (94.9%)]. These results suggest that the sequence homology of the 18S rRNA gene of the two C. mesnili isolates from Japanese macaques was similar to that of humans, in addition to the morphological similarity, and Retortamonas sp. infection of the amphibian type in the small Indian mongoose highlighted the possibility of the effect of host feeding habitats.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e011923"},"PeriodicalIF":1.3,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10704869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Component communities and annual and seasonal variations of metazoan parasites in Eleotris pisonis (Gmelin, 1789) (Gobiiformes: Eleotridae) in the Amazon River, Brazil. 巴西亚马孙河中棘蝗(gobiformes: Eleotris pisonis, Gmelin, 1789) (gobiformes:棘蝗科)后生动物寄生虫的组成群落和年及季节变化。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023073

Elvis Silva Lima, Marcos Tavares-Dias

{"title":"Component communities and annual and seasonal variations of metazoan parasites in Eleotris pisonis (Gmelin, 1789) (Gobiiformes: Eleotridae) in the Amazon River, Brazil.","authors":"Elvis Silva Lima, Marcos Tavares-Dias","doi":"10.1590/S1984-29612023073","DOIUrl":"10.1590/S1984-29612023073","url":null,"abstract":"Our hypothesis for this study was that annual and seasonal variations do not influence the structure of the component communities and the diversity of metazoan parasites of spinycheek sleeper (Eleotris pisonis) in the Amazon River, state of Amapá, Brazil. A total of 164 fish were collected between 2020 and 2021, from which 888 parasites were found. In 2020, five species of parasites were found (one Nematoda, one Digenea, one Acanthocephala, one Arachnida and one Crustacea); and in 2021, five species were also found (three Nematoda, one Digenea and one Crustacea). Larvae of Contracaecum sp. were the dominant taxon throughout the study. The parasite species richness and Brillouin diversity index were higher in 2021, without significant differences between seasonal periods. Some component communities of parasites showed differences between years and between seasonal periods. These facts do not support the hypothesis that such variables would not influence the component communities of the parasites. Lastly, this report provides the first records of Spirocamallanus inopinatus, Genarchella genarchella, Acari, Ergasilus sp., Neoechinorhynchus sp., larvae of Pseudoproleptus sp. and larvae of Contracaecum sp. in E. pisonis.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e011623"},"PeriodicalIF":1.3,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10704881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A new species of Aplectana Railliet & Henry, 1916 (Nematoda: Cosmocercidae) in the Brazilian Amazon and the taxonomic status of Aplectana longa. 巴西亚马孙河流域一新种(线虫纲:大尾线虫科)及长尾线虫的分类地位。

IF 1.3 4区农林科学

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2023-12-04 eCollection Date: 2023-01-01 DOI: 10.1590/S1984-29612023074

Ana Nunes Santos, Ewerton Dos Santos Borges, Yuri Willkens, Jeannie Nascimento Dos Santos, Carlos Eduardo Costa-Campos, Francisco Tiago de Vasconcelos Melo

{"title":"A new species of Aplectana Railliet & Henry, 1916 (Nematoda: Cosmocercidae) in the Brazilian Amazon and the taxonomic status of Aplectana longa.","authors":"Ana Nunes Santos, Ewerton Dos Santos Borges, Yuri Willkens, Jeannie Nascimento Dos Santos, Carlos Eduardo Costa-Campos, Francisco Tiago de Vasconcelos Melo","doi":"10.1590/S1984-29612023074","DOIUrl":"10.1590/S1984-29612023074","url":null,"abstract":"Nematodes of the genus Aplectana Railliet & Henry, 1916 are common parasites of the digestive tract of amphibians and reptiles in the Neotropical region. During a parasite survey on Boana boans (Linnaeus, 1758), we found specimens of nematodes with Aplectana characteristics. We observed a set of characteristics that differs the species of our study from its congeners, and the present study describes a new species of Aplectana parasite of B. boans using light microscopy and scanning electron microscopy. Aplectana pella n. sp. has lateral alae and somatic papillae in males and females; males have equal short spicules, and the gubernaculum is absent. The arrangement of pairs of caudal papillae also differs from other species (2 precloacal,1 adcloacal, and 5 postcloacal+1 unpaired). In females, the vulva is simple, with non-prominent lips, and equatorial. This is the first record of the genus Aplectana parasitizing B. boans and the 58th species described for this genus. Additionally, we added the precloacal papillae pattern of A. delirae, and based on morphological and morphometric characteristics, we propose the reallocation of Aplectana longa to the genus Oxyascaris.","PeriodicalId":48990,"journal":{"name":"Revista Brasileira De Parasitologia Veterinaria","volume":"32 4","pages":"e014023"},"PeriodicalIF":1.3,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10704876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138499911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0