First report of unusual case of parasitism by Amblyomma nodosum (Neumann, 1889) in a yellow cururu toad (Rhinella icterica) in the Northeastern Brazilian Caatinga.

IF 1.3 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-06-28 eCollection Date: 2024-01-01 DOI:10.1590/S1984-29612024031

Daniel Antônio Braga Lee, Darci Moraes Barros-Battesti, Paulo Vitor Cadina Arantes, Jovêncio Mateus Sada, Gustavo Seron Sanches, Marcos Rogério André, Victor Fernando Santana Lima

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引用次数: 0

Abstract

The Amblyomma genus (Arachnida: Ixodidae) is widely distributed in South America, with 34 species occurring in Brazil. Amblyomma nodosum Neumann 1889 is a species that predominantly feeds on Passeriformes during immature stages (larvae and nymphs) and anteaters (Myrmecophagidae) during adult stages. The aim of the present study is to report, for the first time, an unusual case of parasitism by adults of A. nodosum on a yellow cururu toad (Rhinella icterica) captured in the city of Nossa Senhora da Glória, Sergipe state (Northeastern Brazil) in the Caatinga biome, and also investigate the presence of DNA of Rickettsia in the collected material. DNA was extracted from all specimens collected (N=8) and subjected to PCR assays based on the tick 16S rRNA endogenous gene and gltA gene for Rickettsia sp. All samples (8/8; 100%) were positive for the 16S rRNA endogenous gene and two amplicons (obtained from one male and one female) were purified and sequenced. The BLASTn analysis of the sequences revealed a high degree of similarity (95-100%) with A. nodosum sequences previously deposited on GenBank, while the phylogenetic analysis clustered the sequences obtained in the same clade as A. nodosum sequences from Brazil.

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首次报告巴西东北部卡廷加地区黄库鲁蟾蜍（Rhinella icterica）寄生 Amblyomma nodosum（Neumann，1889 年）的不寻常病例。

Amblyomma 属（蛛形纲：Ixodidae）广泛分布于南美洲，在巴西有 34 个物种。Amblyomma nodosum Neumann 1889 是一种主要在未成熟阶段（幼虫和若虫）以雀形目动物为食，在成虫阶段以食蚁兽（Myrmecophagidae）为食的物种。本研究旨在首次报告 A. nodosum 成虫寄生在卡廷加生物群落中塞尔希培州（巴西东北部）Nossa Senhora da Glória市捕获的黄库鲁蟾蜍（Rhinella icterica）上的不寻常案例，并调查采集材料中立克次体 DNA 的存在情况。所有样本（8/8；100%）的 16S rRNA 内源基因均为阳性，两个扩增子（一雄一雌）已纯化并测序。对序列进行的 BLASTn 分析表明，这些序列与之前存放在 GenBank 上的 A. nodosum 序列具有高度相似性（95%-100%），而系统发生分析则将获得的序列与巴西的 A. nodosum 序列归为同一支系。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.