Absence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil.

IF 1.3 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2024-07-22 eCollection Date: 2024-01-01 DOI:10.1590/S1984-29612024038

Julia Somavilla Lignon, Diego Moscarelli Pinto, Mariana Accorsi Teles, Maira Aparecida Christello Trindade, Priscila Rockenbach Portela, Silvia Gonzalez Monteiro, Kauê Rodriguez Martins, Rodrigo Casquero Cunha, Felipe Geraldo Pappen, Bianca Conrad Bohm, Fábio Raphael Pascoti Bruhn

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Abstract

Leishmaniasis are neglected diseases transmitted by vectors that affect domestic and wild animals, including humans. Due to its incidence and lethality, this zoonosis is a worrying public health problem, making it essential to identify all links in the transmission chain. Infection of wild mammals by Leishmania spp. remains poorly understood, especially in southern Brazil. Therefore, the objective was to research, using the PCR technique, the presence of Leishmania spp. DNA in road-killed wild mammals in Southern Brazil. Carcasses of 96 animals were collected from highways in the Pelotas microregion, Rio Grande do Sul, southern Brazil and subjected to necropsies. Tissue fragments (spleen, skin, liver, kidney, heart, lung, lymph nodes, bone marrow and blood) were collected and genomic DNA was extracted. PCR protocols targeting the ITS1, kDNA and 18S genes were tested. We found no evidence of Leishmania spp. circulation in the studied population. However, epidemiological studies like this one are of great relevance, as they allow monitoring of the occurrence of pathogens and help identify possible risk areas. As these animals act as epidemiological markers for the presence of the microorganism, studies must be carried out continuously to understand whether there are sources of infection in the region.

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巴西南部被路边捕杀的野生哺乳动物体内没有利什曼原虫 DNA。

利什曼病是一种被忽视的疾病，通过病媒传播，影响包括人类在内的家畜和野生动物。由于其发病率和致死率高，这种人畜共患病是一个令人担忧的公共卫生问题，因此必须查明传播链中的所有环节。人们对野生哺乳动物感染利什曼原虫的情况仍然知之甚少，尤其是在巴西南部。因此，我们的目标是利用 PCR 技术研究巴西南部被路边捕杀的野生哺乳动物中是否存在利什曼原虫 DNA。研究人员从巴西南部南里奥格兰德州佩洛塔斯微型地区的高速公路上采集了96只动物的尸体，并对其进行了尸检。收集组织碎片（脾脏、皮肤、肝脏、肾脏、心脏、肺脏、淋巴结、骨髓和血液）并提取基因组 DNA。测试了针对 ITS1、kDNA 和 18S 基因的 PCR 方案。我们没有发现利什曼原虫在研究人群中传播的证据。然而，像这样的流行病学研究具有重要意义，因为它们可以监测病原体的发生，并帮助确定可能的风险区域。由于这些动物是微生物存在的流行病学标记，因此必须持续开展研究，以了解该地区是否存在感染源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.