Archivos Espanoles De Urologia最新文献

{"title":"Role of Metabolic Syndrome in Male Fertility: Is It a Risk Factor?","authors":"Ali Egemen Avcı, Basri Çakıroğlu, Bekir Sami Uyanık, Muammer Kendirci, Mehmet Murad Başar","doi":"10.56434/j.arch.esp.urol.20257805.73","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.73","url":null,"abstract":"<p><strong>Background: </strong>Metabolic syndrome (MetS) is a significant health issue linked to male infertility through mechanisms such as aromatisation, hormonal imbalance, increased scrotal temperature, decreased testicular blood flow and increased DNA damage. This study evaluated the serum hormone levels and sperm parameters in men with idiopathic infertility on the basis of the presence or absence of MetS.</p><p><strong>Methods: </strong>Infertile men were included. Height, weight and waist circumference were measured, and body mass index (BMI) was calculated. Blood pressure and biochemical parameters, including serum hormone levels, blood sugar, lipid profiles and semen analysis after 3-5 days of abstinence, were assessed. Participants were divided into two groups on the basis of the International Diabetes Federation criteria: Those with MetS (group I) and those without MetS (group II). Parameters were statistically compared between groups. Risk factors were assessed using logistic regression.</p><p><strong>Results: </strong>A total of 518 infertile men were included, of whom 227 (43.8%) had MetS (group I) and 291 (56.2%) did not (group II). Significant differences were observed in all MetS criteria except low-density lipoprotein levels (<i>p</i> = 0.240). Serum testosterone levels were significantly lower in group I (<i>p</i> = 0.001). Although sperm parameters were lower in group I, but the differences were not statistically significant (<i>p</i> > 0.05). Age (odds ratio (OR): 1.075; 95% confidence interval (CI): 1.042-1.109; <i>p</i> < 0.001), medication use (OR: 1.675; 95% CI: 1.131-2.481; <i>p</i> = 0.01) and ejaculate volume (OR: 0.869; 95% CI: 0.772-0.978; <i>p</i> = 0.02) were found to be risk factors for MetS in regression analysis.</p><p><strong>Conclusions: </strong>MetS may negatively influence the male reproductive system through various mechanisms. Further research is required to clarify the effect of MetS on male infertility.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"541-546"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Remimazolam or Propofol Combined with Sufentanil for Prostate Biopsy in Elderly Men: A Single-Centre, Retrospective Study.","authors":"Geng Liu, Yuzhou Yu","doi":"10.56434/j.arch.esp.urol.20257805.79","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.79","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the efficacy and safety of different anaesthetic drugs in prostate biopsy.</p><p><strong>Methods: </strong>The study included patients who underwent prostate biopsy at our hospital from January 2023 to January 2024. All patients received ultrasound-guided transrectal prostate biopsy. The mean arterial pressure (MAP) and heart rate (HR) of the two groups were observed at seven time points: Entering the operating room (T0), starting drug injection (T1), 3 min (T2), 5 min (T3) after drug injection, the end of the operation (T4), entering the PACU (T5) and leaving the PACU (T6). The time from induction to loss of consciousness (A1), the time from induction to bispectral index (BIS) dropping to 60 or lower (A2), the time from drug withdrawal to eye opening (A3), the length of postoperative post-anaesthesia care unit (PACU) stay (A4) and the operation time (A5) were observed. Moreover, the safety of the two groups was compared.</p><p><strong>Results: </strong>A total of 76 patients were enrolled and divided into two groups: The remimazolam combined with sufentanil (RS) group (n = 37) and the propofol combined with sufentanil (PS) group (n = 39). No significant differences were observed in general data, A1, A2, A3, A4 or A5 between the groups (<i>p</i> > 0.05). Repeated measures analysis of variance (ANOVA) demonstrated significant interaction effects between measurement time and group on MAP and HR (<i>p</i> < 0.05). In addition, significant main effects were observed for time and group (<i>p</i> < 0.05). Simple effect analysis showed no significant differences in MAP and HR at T0 and T1 (<i>p</i> > 0.05). At T2, T3, T4, T5 and T6, the MAP in the RS group was higher than in the PS group (<i>p</i> < 0.05). The HR in the RS group was higher at T2, T3 and T4 (<i>p</i> < 0.05), but exhibited no differences at T5 and T6 (<i>p</i> > 0.05). In terms of safety, the RS group had lower incidences of injection pain and low blood pressure than the PS group (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>Remimazolam combined with sufentanil provides more stable vital signs and improved safety in elderly men undergoing prostate biopsy, rendering it worthy of promotion.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"588-594"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of 3D Reconstruction and Printing on Surgical Outcomes in Renal Tumour Surgery.","authors":"Daniel Campos-Valverde, José Ramón Muñoz-Rodríguez, Paz Castro Fernández, David López Sánchez, Gonzalo Bueno Serrano, Francisco J Damas Arroyo, Manuel Pérez-Utrilla, Santiago Pérez Cadavid, David Grande Murillo, Ana García Torres, David Higuero Acevedo, María Casa Valencia, María Pilar Alcoba García, Alba Lara Isla, Alejandro Puerto Puerto, Carmen Gonzalez Enguita, Francisco Javier Redondo Calvo","doi":"10.56434/j.arch.esp.urol.20257805.72","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.72","url":null,"abstract":"<p><strong>Background and purpose: </strong>With the advances in imaging technology that have enabled the widespread adoption of nephron-sparing surgical techniques, the incidence of renal tumours has concurrently risen. Current guidelines recommend partial nephrectomy for all tumours smaller than 7 cm and, when feasible, even for larger lesions. However, the increasing use of nephron-sparing surgery and advancements in imaging have highlighted the substantial variability in renal hilar anatomy, which poses challenges during surgical procedures. Three-dimensional (3D) imaging technology integrated into preoperative planning has been proposed as a complement to conventional radiological studies to address this complexity. This study aims to evaluate the impact of preoperative 3D imaging and printing on the surgical management of renal cancer.</p><p><strong>Methods: </strong>A prospective cohort study was conducted involving 37 patients managed according to standard clinical protocols. The patients were divided into two groups: One group underwent preoperative 3D tumour reconstruction and printing, and the other did not. Key surgical outcomes, including operative time, intraoperative blood loss, need for transfusion and status of surgical margins on pathological examination, were assessed. All 3D models were reconstructed and printed using polylactic acid (PLA) by the same urologist to ensure consistency.</p><p><strong>Results: </strong>The group that received preoperative 3D reconstruction demonstrated remarkable reductions in intraoperative blood loss and operative time, with no cases of positive surgical margins. No significant differences in tumour size, renal nephrometry index or surgery performed were observed between the two groups.</p><p><strong>Conclusions: </strong>The incorporation of 3D printing and reconstruction into the surgical planning of renal tumours offers tangible benefits, including reduced intraoperative blood loss, short operative duration and enhanced oncological outcomes by minimising the risk of positive margins. These findings support the potential of 3D technology as a valuable adjunct to traditional preoperative assessment in oncological renal surgery.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"534-540"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urogenital Function and Oncologic Outcomes after Nerve-Sparing Radical Cystectomy: A Systematic Review and Meta-Analysis.","authors":"Heyi Hu, Bo Liu","doi":"10.56434/j.arch.esp.urol.20257805.81","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.81","url":null,"abstract":"<p><strong>Objective: </strong>This systematic review and meta-analysis aimed to determine the impact of nerve-sparing radical cystectomy (NS RC) on genitourinary function and oncologic outcomes in patients with bladder cancer (BCa).</p><p><strong>Methods: </strong>We conducted a systematic search for literature related to the treatment of BCa with NS RC and non-NS RC in PubMed, Cochrane Library, Web of Science, and China National Knowledge Infrastructure. Two independent researchers screened the literature according to pre-established inclusion and exclusion criteria and had the literature assessed for quality. After extracting data from eligible ones, the research team performed a meta-analysis using Review Manager (RevMan) 5.4.1. They further conducted Begg's test using Stata 17 to assess publication bias.</p><p><strong>Results: </strong>A total of 261 articles were retrieved. Nine studies met the inclusion criteria, and 740 patients were enrolled in the study. The surgical procedures in the intervention group included prostate capsule sparing cystectomy, prostate-sparing cystectomy, and NS RC. Five studies evaluated urinary incontinence after surgery. After excluding one with a significant source of heterogeneity, the pooled risk ratio (RR) of the four studies was 0.56 (95% confidence interval (CI) (0.35-0.89), <i>p</i> = 0.01). Four studies evaluated erectile dysfunction after surgery. After excluding one with obvious heterogeneity, the pooled RR of the three studies was 0.54 (95% CI (0.36-0.83), <i>p</i> = 0.005). Four studies evaluated the cancer survival rate, and the pooled RR of the four studies was 1.08 (95% CI (0.97-1.21), <i>p</i> = 0.16). Two studies evaluated incisal positive, and the pooled RR of the two studies was 0.83 (95% CI (0.28-2.46), <i>p</i> = 0.74). Four studies evaluated recurrence or metastasis, and the pooled RR of the four studies was 0.54 (95% CI (0.25-1.14), <i>p</i> = 0.10).</p><p><strong>Conclusions: </strong>NS RC can reduce the risk of urinary incontinence and erectile dysfunction in patients with BCa after surgery, and it has no significant effect on oncologic outcomes.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"604-612"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Value of ¹⁸F-FDG and ¹⁸F-PSMA-1007 PET/CT in Bone Metastases from Prostate Cancer: Retrospective Comparative Analysis.","authors":"Qingyun Wang, Lingmei Wu, Fengxiao Gao","doi":"10.56434/j.arch.esp.urol.20257805.77","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.77","url":null,"abstract":"<p><strong>Objective: </strong>The early and accurate diagnosis of prostate cancer (PCa) bone metastases is of great importance for formulating the best treatment plan and improving the prognosis of patients. This work aimed to compare and analyse the application value of ¹⁸F-fludeoxyglucose (FDG) and ¹⁸F-prostate-specific membrane antigen-1007 (¹⁸F-PSMA-1007) positron emission tomography/computed tomography (PET/CT) in the diagnosis of PCa bone metastases.</p><p><strong>Methods: </strong>Data from the pathological specimens from patients diagnosed with PCa admitted to the Xingtai People's Hospital from October 2021 to October 2024 were retrospectively collected. These patients underwent ¹⁸F-FDG PET/CT and ¹⁸F-PSMA-1007 PET/CT scans, with an interval not exceeding 3 days between the two examinations. The sensitivity, specificity, negative and positive predictive values and diagnostic coincidence rate of the two imaging methods were evaluated by comparing the lesion count and anatomical distribution of prostatic bone metastases after the two examinations. The pathological diagnosis results of prostate lesion needle biopsy or radical PCa resection specimens were taken as the gold standard.</p><p><strong>Results: </strong>Seventy-eight patients with PCa were admitted during the study. A total of 418 positive lesions, 78 primary prostatic lesions, 340 bone metastases and 12 negative lesions were detected by prostate lesion biopsy as the gold standard. A total of 410 positive foci were found in ¹⁸F-PSMA-1007, including 78 primary prostatic foci and 332 bone metastases. A total of 376 positive lesions, 76 prostate lesions and 300 bone metastases were found in ¹⁸F-FDG. ¹⁸F-PSMA-1007 detected more PCa bone metastases in different bone regions than ¹⁸F-FDG, but the difference was not statistically significant (<i>p</i> > 0.05). The sensitivity, specificity, positive and negative predictive values and diagnostic coincidence rate of ¹⁸F-PSMA-1007 were significantly higher than those of ¹⁸F-FDG (<i>p</i> < 0.01).</p><p><strong>Conclusions: </strong>¹⁸F-PSMA-1007 PET/CT has high sensitivity and diagnostic coincidence rate and is expected to become a new standard for the diagnosis of PCa bone metastases.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"572-578"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Mobile App-Based Pelvic Floor Combined with Hip Muscle Training on Stress Urinary Incontinence in Women.","authors":"Yan Tang, Liang Yang, Zegang Hu, Li Shen, Duomei Zhang, Lei Jia","doi":"10.56434/j.arch.esp.urol.20257805.82","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.82","url":null,"abstract":"<p><strong>Objective: </strong>This study was designed to evaluate the impact of mobile app-based pelvic floor combined with hip muscle training on female stress urinary incontinence (SUI).</p><p><strong>Methods: </strong>The clinical data of female patients with SUI treated in our hospital from January 2021 to December 2023 were retrospectively collected. They were divided into the control group and combined group according to the difference in treatment style. The control group received conventional pelvic floor muscle training (PFMT) treatment, and the combined group received mobile app-based PFMT combined with hip muscle training treatment.</p><p><strong>Results: </strong>A total of 113 patients were included (55 in the control group and 58 in the combination group). Their general data were comparable (<i>p</i> > 0.05). Repeated measures analysis of variance (ANOVA) revealed a significant interaction between measurement time and group on International Consultation on Incontinence Questionnaire-Short Form (ICIQ-SF) (<i>p</i> < 0.05), with significant main effects of time and group (<i>p</i> < 0.001). Simple effect analysis showed no significant ICIQ-SF difference between the groups before training (<i>p</i> > 0.05). At 8 and 12 weeks, the combination group had a lower ICIQ-SF than the control group (<i>p</i> < 0.05). Before exercise, we found no significant difference in 1-hour PAD between the groups (<i>p</i> > 0.05); After exercise, the combination group outperformed the control group (<i>p</i> < 0.05). Post-exercise, the combination group had a lower incontinence episode frequency (IEF) and higher electromyography parameters than the control group (<i>p</i> < 0.05). The combination group's treatment compliance was significantly better than the control group's (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>Mobile application-based pelvic floor and hip muscle training can effectively reduce urinary incontinence symptoms, improve disease severity, decrease incontinence frequency, enhance pelvic floor function and ensure high treatment compliance in female patients with SUI, making it a promising clinical approach.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"613-620"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of Current Non-Invasive Peyronie's Disease Management: A Changing Reality.","authors":"Jaime Garre-Hernanz, María Moyano-Maqueda, José Marenco, Borja García-Gómez","doi":"10.56434/j.arch.esp.urol.20257805.71","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.71","url":null,"abstract":"<p><p>Peyronie's disease (PD) is a fibrotic disorder that leads to plaque formation in 2%-9% of adult men. It causes a penile curvature that may hamper sexual intercourse. Treatment for PD may be challenging for urologists because of the multiplicity of treatment options available. Several clinical trials and case series have reported significant benefits in the treatment of PD. However, despite continuous updates to recommendations by international organisations, the available data remain limited due to the lack of randomisation, small cohort sizes, and poor patient adherence to therapy. Consequently, the level of evidence supporting recommendations for the andrology community remains low. This review aims to provide a comprehensive, evidence-based overview of current non-invasive treatment modalities for PD and highlights recent clinical practices and relevant clinical trials. We reviewed current clinical practice and ongoing clinical trials for the management of PD. Current treatment options for PD include oral drugs, topical approaches, intralesional injections, traction therapy and penile surgery which remains the gold standard. Intralesional therapy delivers the best evidence for non-invasive treatment. <i>Collagenase Clostridium Histolyticum</i> (<i>CCH</i>) is the only Food and Drug Administration (FDA)-approved treatment available, and its withdrawal has removed a promising first-line treatment option for PD. Emerging evidence suggests that penile traction therapy may enhance treatment outcomes and is a promising complementary approach in future therapeutic strategies.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"525-533"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Improvement of the Ability of Young Urologists for the Basic Teaching Skills Competition through a 'Three-Stage and Three-Level' Progressive Preparation Strategy.","authors":"Ben Xu, Hui Yi, Jiaen Zhang, Lin Ye, Changwei Yuan","doi":"10.56434/j.arch.esp.urol.20257805.74","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.74","url":null,"abstract":"<p><strong>Background: </strong>This study aims to explore the development and improvement of the ability of young urologists for the basic teaching skills competition through a 'three-stage and three-level' (three-stage: The preclass stage, the class stage and the postclass stage; Three-level: Establishing rules, distinctive features and improvement) progressive preparation strategy.</p><p><strong>Methods: </strong>Lecture videos from our researcher and six young urologists were selected. The videos before and after the 'three-stage and three-level' progressive preparation strategy was implemented were submitted to expert judges and students for scoring, and the course scores (100 points) including lesson plan evaluation (30 points), expert judge evaluation (45 points), student judge evaluation (20 points) and teaching reflection evaluation (5 points) were compared. A scoring scale was used to evaluate the professional identity and occupational burnout of young urologists before and after the strategy was adopted.</p><p><strong>Results: </strong>After the 'three-stage and three-level' progressive preparation strategy was implemented, the course scores of the researcher and other urologists were significantly higher than the preimplementation scores (<i>p</i> < 0.001). In addition, the professional identity scores of young urologists were significantly higher than those before implementation (<i>p</i> < 0.01). Amongst young urologists, the scores of emotional exhaustion, depersonalisation and personal achievement after implementation were statistically different (<i>p</i> < 0.05) from those before implementation.</p><p><strong>Conclusions: </strong>A 'three-stage and three-level' progressive preparation strategy can significantly improve the ability of young urologists for the basic teaching skills competition, enhance their professional identity and significantly alleviate occupational burnout.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"547-554"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MYB Proto-Oncogene Like 2 Promotes the Growth of Renal Carcinoma by Attenuating Endoplasmic Reticulum Stress and Activating Lipid Rafts.","authors":"Xiaoxi Zhang, Baixin Liu, Chunlai Cheng, Kui Yang, Dailong Li, Yuan Che","doi":"10.56434/j.arch.esp.urol.20257805.80","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.80","url":null,"abstract":"<p><strong>Objective: </strong>Renal cell carcinoma (RCC) is a typical malignant lesion of kidney with high recurrence rate and high mortality. MYB proto-oncogene-like 2 (<i>MYBL2</i>) is significantly expressed in cancer cells and closely linked to cancer development and poor prognosis. This study aimed to investigate the role and possible mechanisms of <i>MYBL2</i> in renal cancer.</p><p><strong>Method: </strong><i>MYBL2</i> was silenced and overexpressed in A498 kidney cancer cells. The ability of A498 cells to migrate and proliferate was analysed by 5-ethynyl-2'-deoxyuridine staining, colony formation assay, Transwell assay and cell counting kit-8 assay. Endoplasmic reticulum stress was assessed by boron-dipyrromethene (BODIPY) staining, and the messenger ribonucleic acid (mRNA) levels of endoplasmic reticulum (ER) stress markers were determined. Cholesterol levels in A498 cells were analysed by measuring total cholesterol, free cholesterol and Filipin III staining. Sphingomyelin levels were measured, and cholera toxin subunit B (CT-B) staining was used to analyse the stability of lipid rafts. The relationship between <i>MYBL2</i> and ER stress was confirmed using the ER stress inhibitor 4-phenylbutyric acid.</p><p><strong>Results: </strong><i>MYBL2</i> promotes the proliferation and migration of A498 cells (<i>p</i> < 0.01), inhibits the glucose regulated protein 78kD (GRP78)-protein kinase r-like endoplasmic reticulum kinase (PERK)-eukaryotic initiation factor 2 alpha (eIF2α)-activating transcription factor 4 (ATF4)-C/EBP-homologous protein (CHOP) axis (<i>p</i> < 0.01) and ER stress (<i>p</i> < 0.001), upregulates cholesterol levels and increases lipid raft stability (<i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>This study highlights the potential of <i>MYBL2</i> as a therapeutic target for renal cancer. It is a novel oncogene that stimulates the growth of renal cancer cell A498 by reducing ER stress and triggering lipid raft regulation.</p>","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"595-603"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Letter to the Editor Re: Nursing Collaboration and Complication Prevention in Ultrasound-Guided Prostate Biopsy.","authors":"Yan Dong, Wenbin Zhang, Sumei Xiu, Qiming Niu","doi":"10.56434/j.arch.esp.urol.20257805.85","DOIUrl":"https://doi.org/10.56434/j.arch.esp.urol.20257805.85","url":null,"abstract":"","PeriodicalId":48852,"journal":{"name":"Archivos Espanoles De Urologia","volume":"78 5","pages":"630-631"},"PeriodicalIF":0.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144610097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}