Bio-Design and Manufacturing最新文献

{"title":"Concurrently bioprinted scaffolds with autologous bone and allogeneic BMSCs promote bone regeneration through native BMSC recruitment","authors":"Yu Huan, Hongqing Chen, Dezhi Zhou, Xin He, Sanzhong Li, Xiuquan Wu, Bo Jia, Yanan Dou, Xiaowei Fei, Shuang Wu, Zhou Fei, Tao Xu, Fei Fei","doi":"10.1631/bdm.2400011","DOIUrl":"https://doi.org/10.1631/bdm.2400011","url":null,"abstract":"Autologous bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to promote osteogenesis; however, the effects of allogeneic BMSCs (allo-BMSCs) on bone regeneration remain unclear. Therefore, we explored the bone regeneration promotion effect of allo-BMSCs in 3D-printed autologous bone particle (ABP) scaffolds. First, we concurrently printed scaffolds with polycaprolactone, ABPs, and allo-BMSCs for appropriate support, providing bioactive factors and seed cells to promote osteogenesis. In vitro studies showed that ABP scaffolds promoted allo-BMSC osteogenic differentiation. In vivo studies revealed that the implantation of scaffolds loaded with ABPs and allo-BMSCs into canine skull defects for nine months promoted osteogenesis. Further experiments suggested that only a small portion of implanted allo-BMSCs survived and differentiated into vascular endothelial cells, chondrocytes, and osteocytes. The implanted allo-BMSCs released stromal cell-derived factor 1 through paracrine signaling to recruit native BMSCs into the defect, promoting bone regeneration. This study contributes to our understanding of allo-BMSCs, providing information relevant to their future application.","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"8 1","pages":"85-99"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147889169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deep phenotyping of testosterone-prompted fibrosis in arrhythmogenic right ventricular cardiomyopathy using iPSC-derived engineered cardiac spheroids","authors":"Hongyi Cheng, Xinrui Wang, Si-chong Qian, Yike Zhang, Jincheng Jiao, Bingyu Zheng, Yue Zhu, Hua Xu, Jia Song, Feng Zhang, Xiaohong Jiang, Chang Cui, Minglong Chen","doi":"10.1631/bdm.2400168","DOIUrl":"https://doi.org/10.1631/bdm.2400168","url":null,"abstract":"Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a progressive disease characterized by adipose and fibrous replacement of the myocardium. While elevated testosterone levels have been implicated in the pathological process of ARVC, its exact contribution to cardiac fibrosis in ARVC remains unclear. In this study, we analyzed the potential contribution of gender-based differences on the distribution of the low-voltage area in an ARVC cohort undergoing an electrophysiological study, which was indicated by feature selection. Additionally, we established engineered cardiac spheroid models in vitro using patient-specific induced pluripotent stem cell (iPSC)-derived cardiomyocytes (iPSC-CMs) and iPSC-derived cardiac fibroblasts (icFBs). We elucidated the pathogenicity of abnormal splicing in the plakophilin-2 (PKP2) gene caused by an intronic mutation. Additionally, pathogenic validation of the desmoglein-2 (DSG2) point mutation further confirms the reliability of the models. Moreover, testosterone exacerbated the DNA damage in the mutated cardiomyocytes and further activated myofibroblasts in a chain reaction. In conclusion, we designed and constructed an in vitro three-dimensionally-engineered cardiac spheroid model of ARVC based on clinical findings and provided direct evidence of the fibrotic role of testosterone in ARVC.","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"8 1","pages":"20-35"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting of a dermal scaffold for full-thickness skin tissue regeneration","authors":"Lu Han, Zixian Liu, Meng Li, Zhizhong Shen, Jianming Wang, Shengbo Sang","doi":"10.1631/bdm.2400058","DOIUrl":"https://doi.org/10.1631/bdm.2400058","url":null,"abstract":"Dermal substitutes have provided a template for the regeneration and reconstruction of the dermis. However, the healed skin tissue often exhibits abnormal morphology and functionality, including scarring and inflammation. In this study, a composite bioink composed of methacrylated gelatin (GelMA) and chitosan oligosaccharide (COS) was proposed for printing a dermal scaffold using digital light processing (DLP) technology. The GelMA/COS bioink exhibited suitable porosity, swelling, degradation rate, and mechanical properties. The inclusion of COS demonstrated antibacterial effects against both Grampositive and Gram-negative bacteria, while simultaneously fostering the proliferation of human dermal fibroblasts (HDFs). Additionally, the application of COS could effectively reduce the expression levels of fibrosis-related genes, such as collagen I, collagen III, and fibronectin I. The three-dimensionally printed cell-laden dermal scaffold exhibited excellent shape fidelity and high cellular viability, facilitating the extension of HDFs along the scaffold and the simultaneous secretion of extracellular matrix proteins. Furthermore, the HDF-laden dermal scaffold transplanted into full-thickness skin defect sites in nude mice was shown to accelerate wound closure, reduce inflammation, and improve wound healing. Overall, the DLP-printed dermal scaffold provides an appealing approach for effectively treating full-thickness skin defects in clinical settings.","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"8 1","pages":"68-84"},"PeriodicalIF":0.0,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147908383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal MXene-embedded tannin–Eu3+ particles as in situ bacterial vaccines for accelerating healing of wounds infected by seawater immersion and providing persistent anti-infection effects","authors":"Zhentao Li, Ting Song, Yanpeng Jiao, Zijing Zhu, Yang Liao, Zonghua Liu","doi":"10.1631/bdm.2300327","DOIUrl":"https://doi.org/10.1631/bdm.2300327","url":null,"abstract":"The low temperature, high salt content, and bacterial composition of a seawater environment can induce severe infections in open wounds, thus impending wound healing. To date, numerous wound dressings have been developed to injuries and various antibacterial functions. However, ordinary antibacterial strategies could not provide long-term resistance to infections and could not promote wound healing. Here, we proposed a strategy for enhancing resistance to seawater immersion wound infection by killing bacteria and delivering bacterial antigens in situ. Specifically, MXene-embedded tannin–Eu3+ (M@TA–Eu) particles were constructed to effectively alleviate injuries infected through seawater immersion and persistently fight infection by forming in situ bacterial vaccines and immune memory. In the particle, the platform composed of TA and Eu3+ exhibited provascularization and antigen presentation effects. MXenes with near-infrared photothermal effect were introduced to kill the bacteria, promote the recruitment of antigen presentation cells, and ultimately enhance vaccination efficacy. Experimental results showed that the particles not only effectively accelerated the healing of injuries by relieving wound inflammation and inhibiting bacteria but also produced a potent vaccination by forming in situ bacterial vaccines. Therefore, the M@TA–Eu particles are novel materials for high-grade anti-infection dressing.","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"8 1","pages":"116-133"},"PeriodicalIF":0.0,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147917836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnesium and gallium-coloaded microspheres accelerate bone repair via osteogenesis and antibiosis","authors":"Jin Bai, Si Shen, Yan Liu, Shendan Xu, Leo Li, Zirou Wang, Weili Liu, Lingling Pu, Gang Chen, Xinxing Wang","doi":"10.1631/bdm.2300253","DOIUrl":"https://doi.org/10.1631/bdm.2300253","url":null,"abstract":"Bone defects have serious economic and clinical impacts; however, despite improvements in bone defect management, the range of clinical outcomes remains limited. A variety of biomaterials have been used to treat complex bone defects. However, final bone repair outcomes may be adversely affected by poor osteogenic capacity and risk of infection. Consequently, therapeutic methods are required that reduce bacterial contamination and increase the use of osteogenic biomaterials. Herein, we report the preparation of poly(lactic acid-coglycolic acid) (PLGA) microspheres coloaded with magnesium (Mg2+) and gallium (Ga3+) ions (Mg-Ga@PLGA), which can fill irregular bone defects and show good biosafety. During in vitro testing, Mg-Ga@PLGA not only showed a synergistic effect on promoting osteogenic differentiation but also inhibited osteoclastic differentiation. Moreover, we found that Mg-Ga@PLGA demonstrated an antibacterial effect. During in vivo testing, Mg-Ga@PLGA exhibited strong in situ osteogenic ability. In conclusion, Mg-Ga@PLGA has good potential for treating bone defects at risk of infection.","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"8 1","pages":"150-165"},"PeriodicalIF":0.0,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147915279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cav3.3-mediated endochondral ossification in a three-dimensional bioprinted GelMA hydrogel","authors":"Zhi Wang, Xin Wang, Yang Huang, Junjun Yang, Zu Wan, Zhenlan Fu, Xiaoyuan Gong, Guangxing Chen, Liu Yang","doi":"10.1007/s42242-024-00287-1","DOIUrl":"https://doi.org/10.1007/s42242-024-00287-1","url":null,"abstract":"软骨内成骨 软骨内成骨 (EO) 是生长板组织内介导长骨发育的重要生物学过程. 骨类器官能够模拟天然生长板中组织结构与细胞间通信的生理功能, 是一种潜在的长骨发育研究模型. 但如何有效模拟EO过程一直是骨类器官构建中尚未解决的核心难题. 为了模拟EO这一有序的矿化过程, 本文首次揭示了T型电压依赖性钙通道 (T-VDCC) 的Ca v 3.3亚型在EO过程中主要表达于软骨细胞, 并且与软骨细胞的肥大分化呈负相关这一生物学机制, 并基于此机制设计了负载shCa v 3.3 ATDC5细胞的明胶甲基丙烯酰 (GelMA) 水凝胶生物墨水, 且进一步评估了这种生物墨水的生物打印性能. 最后, 本研究通过负载Ca v 3.3敲低的软骨细胞构建了GelMA水凝胶模型, 成功在体外模拟了EO过程, 为构建分层有序矿化的骨类器官提供了一种新型的生物墨水. 因此, 本研究不仅为骨类器官构建中的EO进程调控提供了潜在的研究靶点, 也为模拟EO过程提供了新的策略.","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"7 6","pages":"983-999"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147899772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photocrosslinkable human amniotic membrane hydrogel for recovery from spinal cord injury","authors":"Tao Xu, Changwei Yang, Yang Lu, Heng Wang, Cheng Chen, Yuchen Zhou, Xiaoqing Chen","doi":"10.1007/s42242-024-00318-x","DOIUrl":"https://doi.org/10.1007/s42242-024-00318-x","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"7 6","pages":"1035-1052"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147883677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light/pH dual controlled drug release \"nanocontainer\" alleviates tumor hypoxia for synergistic enhanced chemotherapy, photodynamic therapy and chemodynamic therapy","authors":"Shihe Liu, Xin Zhang, Zhimin Bai, Yi-Bo Yang, Jia Zhang, Kun Li, Zhiwei Liu, Ming Shi, Lixin Dong, Jidong Wang, Jian Li","doi":"10.1007/s42242-024-00310-5","DOIUrl":"https://doi.org/10.1007/s42242-024-00310-5","url":null,"abstract":"","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"7 6","pages":"955-971"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-based 3D printing of stimulus-responsive hydrogels for miniature devices: recent progress and perspective","authors":"Chen Xin, Neng Xia, Li Zhang","doi":"10.1007/s42242-024-00295-1","DOIUrl":"https://doi.org/10.1007/s42242-024-00295-1","url":null,"abstract":"<p>Miniature devices comprising stimulus-responsive hydrogels with high environmental adaptability are now considered competitive candidates in the fields of biomedicine, precise sensors, and tunable optics. Reliable and advanced fabrication methods are critical for maximizing the application capabilities of miniature devices. Light-based three-dimensional (3D) printing technology offers the advantages of a wide range of applicable materials, high processing accuracy, and strong 3D fabrication capability, which is suitable for the development of miniature devices with various functions. This paper summarizes and highlights the recent advances in light-based 3D-printed miniaturized devices, with a focus on the latest breakthroughs in light-based fabrication technologies, smart stimulus-responsive hydrogels, and tunable miniature devices for the fields of miniature cargo manipulation, targeted drug and cell delivery, active scaffolds, environmental sensing, and optical imaging. Finally, the challenges in the transition of tunable miniaturized devices from the laboratory to practical engineering applications are presented. Future opportunities that will promote the development of tunable microdevices are elaborated, contributing to their improved understanding of these miniature devices and further realizing their practical applications in various fields.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"1 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142249209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced axonal regeneration and functional recovery of the injured sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous scaffolds","authors":"Banafsheh Dolatyar, Bahman Zeynali, Iman Shabani, Azita Parvaneh Tafreshi, Reza Karimi-Soflou","doi":"10.1007/s42242-024-00304-3","DOIUrl":"https://doi.org/10.1007/s42242-024-00304-3","url":null,"abstract":"<p>Increasing evidence indicates that engineered nerve grafts have great potential for the regeneration of peripheral nerve injuries (PNIs). While most studies have focused only on the topographical features of the grafts, we have considered both the biophysical and biochemical manipulations in our applied nanoscaffold. To achieve this, we fabricated an electrospun nanofibrous scaffold (ENS) containing polylactide nanofibers loaded with lithium (Li) ions, a Wnt/<i>β</i>‐catenin signaling activator. In addition, we seeded human adipose-derived mesenchymal stem cells (hADMSCs) onto this engineered scaffold to examine if their differentiation toward Schwann-like cells was induced. We further examined the efficacy of the scaffolds for nerve regeneration in vivo via grafting in a PNI rat model. Our results showed that Li-loaded ENSs gradually released Li within 11 d, at concentrations ranging from 0.02 to (3.64 ± 0.10) mmol/L, and upregulated the expression of Wnt/<i>β</i>-catenin target genes (<i>cyclinD1</i> and <i>c-Myc</i>) as well as those of Schwann cell markers (growth-associated protein 43 (GAP43), S100 calcium binding protein B (S100B), glial fibrillary acidic protein (GFAP), and SRY-box transcription factor 10 (SOX10)) in differentiated hADMSCs. In the PNI rat model, implantation of Li-loaded ENSs with/without cells improved behavioral features such as sensory and motor functions as well as the electrophysiological characteristics of the injured nerve. This improved function was further validated by histological analysis of sciatic nerves grafted with Li-loaded ENSs, which showed no fibrous connective tissue but enhanced organized myelinated axons. The potential of Li-loaded ENSs in promoting Schwann cell differentiation of hADMSCs and axonal regeneration of injured sciatic nerves suggests their potential for application in peripheral nerve tissue engineering.</p><h3 data-test=\"abstract-sub-heading\">Graphic abstract</h3>\u0000","PeriodicalId":48627,"journal":{"name":"Bio-Design and Manufacturing","volume":"25 1","pages":""},"PeriodicalIF":7.9,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142188767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}