Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献

{"title":"Procoagulant Properties of Extracellular Vesicles in Normal and Pathological Pregnancy","authors":"E. M. Koltsova,&nbsp;A. A. Martyanov,&nbsp;N. A. Podoplelova","doi":"10.1134/S1990747822060071","DOIUrl":"10.1134/S1990747822060071","url":null,"abstract":"<p>Extracellular vesicles are lipid bilayer membrane structures without nuclei that are released from various cells as a result of physiological and metabolic changes. They play an important role in intercellular communication through the transfer of a wide range of bioactive molecules, contributing to the regulation of various physiological and pathological processes. Extracellular vesicles may have procoagulant properties due to the presence of phosphatidylserine, which accelerates coagulation reactions, on the outer layer of the membrane, as well as the expression of tissue factor, which activates coagulation along the external pathway, on the surface of some vesicles. A large number of clinical and experimental studies have shown that in various pathologies and specific physiological conditions, including pregnancy, the concentration of extracellular vesicles significantly exceeds that in healthy volunteers, which could theoretically be a factor in the development of hypercoagulable states This review focuses on describing the procoagulant properties of extracellular vesicles of various origins in normal and pathological pregnancy.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"12 - 19"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5121224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Juglone and Curcumin Administration on Expression of FABP5 and FABP9 in MCF-7 and MDA-MB-231 Breast Cancer Cell Lines","authors":"D. Soyler,&nbsp;E. N. Korucu,&nbsp;E. Menevse,&nbsp;A. A. Azzawri,&nbsp;D. E. Kaya","doi":"10.1134/S199074782310001X","DOIUrl":"10.1134/S199074782310001X","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Among natural chemopreventive agents, polyphenol curcumin and naphthoquinone juglone, which has broad-spectrum anticancer activity, are highly valued. Fatty acid-binding proteins (FABPs), which vary depending on the type of cancer, are small (14–15 kDa) proteins belonging to the lipid-binding protein superfamily. FABPs are located in many tissues and play an essential role in fatty acid metabolism, cell growth, and proliferation. It is suggested that they can be used as tumor markers. The objective of this work is to study the effects of curcumin and juglone on cell viability and to evaluate their anticancer and cytotoxic effects and changes in the FABP5 and FABP9 gene expression and protein levels in breast cancer cell lines MCF-7 and MDA-MB-231. Information on FABP5 and FABP9 gene expression in BRCA (breast invasive cancer) and normal cells was collected from the GEPIA2 and UALCAN databases. MTT analysis revealed that the IC₅₀ (concentration of half maximal inhibitory effect) in MCF-7 cells was 22.4 and 16.3 μM for curcumin and juglone, respectively, and in MDA-MB-231 cells, 10.4 and 3.4 μM for curcumin and juglone, respectively. In both cell lines, FABP5 and FABP9 gene expression and protein levels were also analyzed. We found that treatment of MCF-7 cells with curcumin and juglone reduced cell viability, expression of the FABP5 and FABP9 genes, and the levels of the FABP5 and FABP9 proteins. In the MDA-MB-231 cell line, the FABP5 and FABP9 levels were increased at low doses of curcumin and juglone and decreased at higher doses.</p></div></div>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"58 - 67"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5127768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dopamine Protects Neurons against Glutamate-Induced Excitotoxicity","authors":"E. I. Fedotova,&nbsp;A. Y. Abramov,&nbsp;A. V. Berezhnov","doi":"10.1134/S1990747822060058","DOIUrl":"10.1134/S1990747822060058","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Parkinson’s disease is associated with neuronal loss in the midbrain and the resulting development of dopamine-deficient states. At the later stages of the disease, increased neuronal death is also observed in other parts of the brain. We hypothesized that dopamine may function as a glutamate antagonist, and dopamine deficiency may increase glutamate-induced excitotoxicity. Using rat hippocampal primary culture and fluorescence microscopy, we show that dopamine reduces the amplitude of calcium response evoked by the activation of NMDA receptors but does not affect calcium signals mediated by AMPA and KA receptors. Voltage-gated calcium channels are also unaffected by dopamine. It was shown that the effect of dopamine depends not only on NMDA receptors, but also on D2-type dopamine receptors and on GABA(A) receptor. Dopamine reduced glutamate-induced mitochondrial depolarization and improved neuronal survival in the presence of toxic levels of glutamate. The data presented suggest a protective role of dopamine against glutamate toxicity.</p></div></div>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"34 - 42"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5124019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myeloid Differentiation Increases Resistance of Leukemic Cells to TRAIL-Induced Death by Reducing the Expression of DR4 and DR5 Receptors","authors":"Ya. V. Lomovskaya,&nbsp;M. I. Kobyakova,&nbsp;A. S. Senotov,&nbsp;I. S. Fadeeva,&nbsp;A. I. Lomovsky,&nbsp;K. S. Krasnov,&nbsp;D. Yu. Shtatnova,&nbsp;V. S. Akatov,&nbsp;R. S. Fadeev","doi":"10.1134/S1990747822060101","DOIUrl":"10.1134/S1990747822060101","url":null,"abstract":"<p>The study of the mechanisms of resistance of tumor cells to TRAIL-induced death remains an urgent task since this cytokine is an important highly selective molecular effector of antitumor immunity. Our study showed that human leukemia cells THP-1, HL-60, and K562 increased their resistance to TRAIL-induced death in vitro as a result of induction of myeloid differentiation in them by exogenous factors in all directions of myelopoiesis, except for erythroid, by reducing the expression of DR4 and DR5 receptors on the cell surface. It was also found that ONC 201, tunicamycin, and SAHA (hydroxamic acid suberoylanilide), capable of causing an increase in the expression of DR5 in leukemic cells, suppressed their TRAIL resistance induced by differentiation factors. The results obtained are of interest for the development of drugs and strategies to improve the effectiveness of the treatment of myeloid leukemia.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"43 - 57"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5124682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversible Increase in Resistance of A-431 Carcinoma Cells to TRAIL-Induced Apoptosis in Confluent Cultures Corresponds to a Decrease in Expression of DR4 and DR5 Receptors","authors":"R. S. Fadeev,&nbsp;N. V. Dolgikh,&nbsp;A. V. Chekanov,&nbsp;A. S. Senotov,&nbsp;K. S. Krasnov,&nbsp;M. I. Kobyakova,&nbsp;Ya. V. Lomovskaya,&nbsp;I. S. Fadeeva,&nbsp;V. S. Akatov","doi":"10.1134/S1990747823100021","DOIUrl":"10.1134/S1990747823100021","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>TRAIL (TNF alpha Related Apoptosis Inducing Ligand) cytokine is of great interest for the development of targeted antitumor drugs. We have previously found a reversible increase in tumour cell resistance to TRAIL-induced apoptosis in confluent cultures. In this work we show that increase in resistance of A-431 cells to TRAIL-induced death in confluent culture is associated with reduced expression of pro-apoptotic receptors DR4 and DR5 with absence of anti-apoptotic receptors DcR1 and DcR2 on cell surface. Decreased representation of DR4 and DR5 receptors on the cell surface is accompanied by a lack of activation of the pro-apoptotic protein Bid, effector caspase 3 under the action of recombinant protein izTRAIL, which leads to an increase in TRAIL resistance. Our results indicate that reversible increase in resistance of human carcinoma A-431 cells to TRAIL-induced apoptosis in confluent cultures is caused by decrease in expression of DR4 and DR5 receptors on cell surface.</p></div></div>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"28 - 33"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5125667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Lipid Domains and Physical Properties of Membranes in the Development of Age-Related Neurodegenerative Diseases","authors":"V. D. Krasnobaev,&nbsp;O. V. Batishchev","doi":"10.1134/S199074782209001X","DOIUrl":"10.1134/S199074782209001X","url":null,"abstract":"<p>A growing number of studies points to the relationship between the development of neurodegenerative diseases and the structure and lipid composition of neuronal membranes. One of the structural elements of cell membranes, to which special attention is paid in this regard, are liquid-ordered lipid domains, or rafts. The study of rafts and age-related changes in the lipid composition of neuronal cells is becoming increasingly relevant and is constantly being updated. In this review, we tried to highlight the possible role of the lipid component of cell membranes, their structure, and physicochemical characteristics in the development of diseases associated with aging. Evidence is reviewed that supports a possible role of rafts in diseases that over a long period of time lead to disruption of the functioning of neurons. There is a reason to believe that the therapeutic effects of various molecules, such as lysolipids and gangliosides, are due to their physicochemical properties and are realized indirectly, through their influence on the organization of lipid domains in membranes. As the role of lipid domains and, in general, the mechanisms of interaction and mutual influence of the lipid composition and disease development are more fully understood, this knowledge can be used to develop new therapeutic or preventive methods to combat diseases associated with aging.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"16 4","pages":"268 - 281"},"PeriodicalIF":0.5,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4378897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Steps in Detection and Interpretation of the Lipid Membrane Boundary Potential","authors":"Yu. A. Ermakov","doi":"10.1134/S1990747822050051","DOIUrl":"10.1134/S1990747822050051","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>The main stages in the development of electrokinetic studies at the Laboratory of Bioelectrochemistry of the Institute of Physical Chemistry, Russian Academy of Sciences, headed by corr. member of RAS Yu.A. Chizmadzhev, are described. Methods of recording and analyzing the results of measuring the electrophoretic mobility of liposomes using dynamic light scattering techniques are briefly overviewed. The advantage of using electrokinetic data in combination with the measurement of the boundary potentials of planar bilayer lipid membranes using the intramembrane field compensation method has been shown. The data obtained in the framework of this approach is illustrated by studies of effects of some membrane-active ions and compounds on the surface and dipole components of the electric field at the lipid membrane interfaces with an aqueous environment.</p></div></div>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"16 4","pages":"261 - 267"},"PeriodicalIF":0.5,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4380541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Mechanisms of Lipid Vesicle Fusion Inhibition by Extracts of Chaga and Buckthorn Leaves","authors":"S. S. Efimova,&nbsp;P. D. Zlodeeva,&nbsp;E. V. Shekunov,&nbsp;O. S. Ostroumova","doi":"10.1134/S199074782205004X","DOIUrl":"10.1134/S199074782205004X","url":null,"abstract":"<p>The ability of extracts of grapefruit seeds (ESG), sea buckthorn leaves (ESBL), and chaga (EC) to inhibit membrane fusion was evaluated. It was found that ESBL and EC inhibited Ca²⁺-mediated fusion of phosphatidylglycerol-enriched lipid vesicles; the inhibition indexes were about 90 and 100%, respectively. ESG did not inhibit the fusion of negatively charged liposomes induced by calcium. In addition to calcium-mediated liposome fusion, EC inhibited the fusion of vesicles from a mixture of phosphatidylcholine and cholesterol under the action of polyethylene glycol with a molecular weight of 8000 Da (the inhibition index was 80%). The other two extracts had no effect on polymer-induced fusion of uncharged membranes. The effect of some major components of the tested extracts on the fusion of vesicles was evaluated. It has been shown that flavonols, quercetin and myricetin, which are major components of ESBL, inhibited the fusion of negatively charged membranes under the action of calcium (the inhibition indexes were about 85 and 60%, respectively). Another flavonol of ESBL, the glycoside of quercetin rutin, did not have such an effect. The data obtained made it possible to relate the ESBL suppression of calcium-induced fusion of lipid vesicles with the presence of quercetin and myricetin in its composition. These flavonols had virtually no effect on polyethylene glycol-induced vesicle fusion, which is consistent with the absence of ESBL action on liposome fusion under the action of polymer. The ability of quercetin and myricetin to reduce the melting temperature of phosphatidylglycerol with saturated hydrocarbon chains and to increase the half-width of the peak corresponding to melting has been demonstrated. The observed correlation between the parameters characterizing the thermotropic behavior of the lipid in the presence of quercetin and myricetin and the index of inhibition of calcium-mediated liposome fusion by these compounds may indicate a relationship between the ability of flavonols to influence the packaging of membrane lipids and inhibit vesicle fusion. Pentacyclic triterpenoids, betulin and lupeol, which are part of EC, did not inhibit the fusion of vesicles under the action of both calcium and polyethylene glycol, and their presence in EC cannot be responsible for the antifusogenic activity of EC.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"16 4","pages":"311 - 319"},"PeriodicalIF":0.5,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S199074782205004X.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4683721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Possibility of Pore Formation in Lipid Membranes by Several Molecules of Amphipathic Peptides","authors":"O. V. Kondrashov,&nbsp;S. A. Akimov","doi":"10.1134/S1990747822050087","DOIUrl":"10.1134/S1990747822050087","url":null,"abstract":"<p>Antimicrobial activity of some amphipathic peptides is associated with the formation of through pores in bacterial membranes. Antimicrobial peptides (AMPs) specifically bind to the plasma membrane by incorporating their hydrophobic regions into the outer lipid monolayer. The membrane is inevitably deformed. Many AMPs form so-called toroidal pores, the edge of which is partially lined with peptide molecules. The edge of the pore is characterized by significant deformations. In this work, we calculated the energy of the pore edge, with amphipathic peptides located on the pore equator, as well as the energy of deformations induced by AMP in a planar lipid bilayer. It was shown that for certain physicochemical and geometric characteristics of the AMP molecule the energy of the pore, on the equator of which two or more peptide molecules are located, can be lower than the energy of deformations induced in the planar bilayer by the same number of peptide molecules. Thus, two AMP molecules can, in principle, form a through pore in the membrane, although this is possible only in a fairly narrow range of physicochemical and geometric characteristics of the peptides.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"16 4","pages":"338 - 350"},"PeriodicalIF":0.5,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4683711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanosensitive Channels: History, Diversity, and Mechanisms","authors":"S. Sukharev,&nbsp;A. Anishkin","doi":"10.1134/S1990747822090021","DOIUrl":"10.1134/S1990747822090021","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>Mechanical forces are inseparable from most cellular functions. Cell division, contraction, and adhesion generate intrinsic forces in the cells, whereas perturbations in the environment such as osmotic shifts, mechanical pressure, shear, or sound represent the external forces that the cells gauge and respond to. Mechanosensitive (MS) ion channels, which are the fastest mechanotransducers, represent a polyphyletic group with vastly diverse structural designs. In this review we briefly outline the history of the field by presenting major findings in a nearly chronological order, describe structural features of different groups, and attempt to illustrate some common physical principles of their gating mechanisms.</p></div></div>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"16 4","pages":"291 - 310"},"PeriodicalIF":0.5,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4379703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}