Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology最新文献

{"title":"Activation of the Cannabinoid Receptors Suppresses Hyperexcitation of Rat Hippocampal Neuronal Networks In Vitro","authors":"S. A. Maiorov,&nbsp;B. K. Kairat,&nbsp;S. G. Gaidin,&nbsp;A. M. Kosenkov,&nbsp;V. P. Zinchenko","doi":"10.1134/S1990747823030078","DOIUrl":"10.1134/S1990747823030078","url":null,"abstract":"<p>Cannabinoid receptors (CBRs) play a key role in various physiological processes, including neurogenesis, synaptic plasticity, immune modulation, cell apoptosis, metabolism regulation, cardiovascular and reproductive systems activity. Since activation of CBRs suppresses hyperexcitation and protects cells from death, their modulation may have therapeutic prospects in the treatment of such pathologies of the nervous system as mental disorders, epilepsy, Parkinson’s and Huntington’s disease, multiple sclerosis, spinal cord and brain injuries. This paper presents experimental data on the effects of the cannabinoid receptor agonist WIN 55,212-2 on the induced oscillations of intracellular Ca²⁺ concentration ([Ca²⁺]_<i>i</i>) in two in vitro models of epileptiform activity. To study the neuroprotective properties of WIN 55,212-2, hyperexcitation was induced by the application of a GABA(A) receptor antagonist, bicuculline, or depolarizing doses of ammonium chloride. As experiments have shown, WIN 55,212-2 at a concentration of 100 nM and above significantly suppresses the [Ca²⁺]_<i>i</i> oscillations frequency and reduces the basal [Ca²⁺]_<i>i</i> level. At the same time, the amplitude of calcium oscillations also decreased in the presence of the agonist. WIN 55,212-2 at a concentration of 2 μM suppressed NH₄Cl-induced [Ca²⁺]_<i>i</i> oscillations in all neurons but caused a transient biphasic increase in the basal [Ca²⁺]_<i>i</i> level in 20% of astrocytes. Thus, in this work, using various models of hyperexcitation of neuronal networks, we have demonstrated a potential antiepileptic effect of the cannabinoid receptor agonist WIN 55,212-2.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"169 - 175"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Changes in the Content of Plasma Membrane and Tonoplast Sterols under Oxidative and Osmotic Stress","authors":"N. V. Ozolina,&nbsp;V. V. Gurina,&nbsp;I. S. Kapustina,&nbsp;E. V. Spiridonova,&nbsp;V. N. Nurminsky","doi":"10.1134/S1990747823020058","DOIUrl":"10.1134/S1990747823020058","url":null,"abstract":"<p>We compared oxidative and osmotic stress-induced changes in sterol content in plasma membranes and tonoplasts isolated from stored beet (<i>Beta</i> <i>vulgaris</i> L.) roots. The most significant differences between the membranes under all studied stresses were observed in the cholesterol content: it decreased in the plasmalemma, but increased 4-6 times in the tonoplast. Similar changes were observed for other sterols, but in different ways under different types of stress. Particularly noticeable differences were noted under hyperosmotic stress. The increase in the content of sterols was much more pronounced in the vacuolar membrane compared to the plasmalemma. This observation allows us to conclude that tonoplast plays a more significant role in protection of the plant cell from stress compared to plasmalemma.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"180 - 182"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4724649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monoclonal Line of Cells Co-Expressing Genetically Encoded Sensors for cAMP and Ca2+","authors":"P. D. Kotova,&nbsp;O. A. Rogachevskaja,&nbsp;S. S. Kolesnikov","doi":"10.1134/S199074782301004X","DOIUrl":"10.1134/S199074782301004X","url":null,"abstract":"<p>A variety of surface receptors and intracellular signaling systems are involved in cell-to-cell communication and paracrine/autocrine regulation of cellular functions. Being most numerous, the family of G‑protein coupled receptors (GPCRs) is involved in the regulation of almost all physiological processes due to coupling to multiple and diverse intracellular signaling cascades. Among them, the ubiquitous contributors are the adenylate cyclase cascade, which controls the intracellular cAMP level, and the phosphoinositide cascade determining many aspects of intracellular Ca²⁺ signaling. Existing evidence suggests that the adenylate cyclase and phosphoinositide cascades are cross regulated. It therefore can be expected that agonists of adenylate cyclase-coupled GPCRs also are capable of affecting intracellular Ca²⁺, and in turn, Ca²⁺-mobilizing ligands could initiate a change in the cAMP level. Thus, simultaneous monitoring of cAMP and Ca²⁺ in the cell cytosol is rational as potentially providing new insights into intracellular signaling processes initiated by agonists. Currently, solely genetically encoded fluorescent sensors enable the on line monitoring of intracellular cAMP, and apart from Ca²⁺ dyes, such sensors are also evaluable for the analysis of intracellular Ca²⁺ signals. Based on maternal HEK-293 cells, here we generated a monoclonal line of HEK-PF/GG cells that co-expressed molecular fluorescent sensors for cAMP (Pink Flamindo) and Ca²⁺ (GEM-GECO1). Physiological tests showed that this cell line provides the possibility of simultaneous monitoring of cAMP and Ca²⁺ with sufficient sensitivity. Such a tool can increase the efficacy of studying agonist-induced intracellular processes and, in particular, the analysis of crosstalk between the cAMP and Ca²⁺ signaling systems.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"176 - 179"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mixed Cationic Liposomes Based on L-Amino Acids As Efficient Delivery Systems of Therapeutic Molecules into Cells","authors":"Z. G. Denieva,&nbsp;O. O. Koloskova,&nbsp;A. M. Gileva,&nbsp;U. A. Budanova,&nbsp;Yu. L. Sebyakin","doi":"10.1134/S1990747823030054","DOIUrl":"10.1134/S1990747823030054","url":null,"abstract":"<p>This work aimed to produce mixed liposomes based on natural amino acids as vehicles for delivery of anticancer drugs and nucleic acids. Liposomes were formed from cationic lipids based on <i>L</i>-alanine and <i>L</i>-serine, a cerasome-forming lipid based on <i>L</i>-ornithine, and phospholipids phosphatidylcholine (PC) or 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphoethanolamine (DOPE). For the developed agents, particle size, zeta potential, and stability were determined, and the biological activity was studied on the MCF-7 and HEK 293 cell lines. Liposomes based on <i>L</i>-serine demonstrated the ability to accumulate in the endoplasmic reticulum of cells within 1 h, and their transfection activity significantly exceeded that of the commercial drug Lipofectamine-2000. At the same time, the proposed system had a slight toxic effect (IC₅₀, 0.475 mg/mL and the safe working concentration, 0.24 mg/mL). Thus, the results suggest that mixed liposomes based on <i>L</i>-serine can serve as an efficient delivery system of drugs into cells.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"136 - 147"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Plant Acclimation on Electron Transport in Chloroplast Membranes of Cucumis sativus and Cucumis melo","authors":"M. A. Benkov,&nbsp;I. S. Suslichenko,&nbsp;B. V. Trubitsin,&nbsp;A. N. Tikhonov","doi":"10.1134/S1990747823030030","DOIUrl":"10.1134/S1990747823030030","url":null,"abstract":"<p>The processes of electron transport in the leaves of two species of plants of the genus <i>Cucumis</i>, the shade-tolerant species <i>Cucumis sativus</i> (cucumber) and the light-loving species <i>Cucumis melo</i> (melon), grown under moderate (50–125 µmol photons m^–2 s^–1) or strong illumination (850–1000 µmol photons m^–2 s^–1) were studied. The parameters of fast and slow induction of chlorophyll <i>a</i> fluorescence were used as indicators characterizing the activity of photosystem 2 (PS2). The functioning of photosystem 1 (PS1) was monitored by changes in the electron paramagnetic resonance signal from the oxidized reaction centers of PS1, <span>({text{P}}_{{700}}^{ + })</span>. A significant difference was revealed in the dynamics of changes in photosynthetic parameters of shade-tolerant (<i>C. sativus</i>) and light-loving (<i>C. melo</i>) species during their acclimation to moderate or high light intensity. In the shade-tolerant species <i>C. sativus</i>, photosynthetic indicators characterizing the activity of PS2 showed a noticeable sensitivity to increased illumination compared to the light-loving species <i>C. melo</i>, indicating a weakening of the activity of PS2 with an increase in light intensity during acclimation of plants. During a long-term (more than 1–2 months) acclimation of <i>C. sativus</i> to high intensity light (≥500 µmol photons m^–2 s^–1), their PS2 lost photochemical activity, which, however, was not observed in the leaves of <i>C. melo</i>. The weakening of the activity of PS2 in the leaves of <i>C. sativus</i> was reversible, that is, after returning to light of moderate intensity, the activity of PS2 was restored to the level characteristic of the leaves of <i>C. melo</i>. In the leaves of plants of both species, differences in the kinetics of photoinduced redox transformations of the reaction centers of PS1 were manifested, depending on the acclimation conditions. In plants acclimated to strong light, there was a clearly pronounced delayed phase of signal from <span>({text{P}}_{{700}}^{ + })</span> growth, which presumably could be caused by cyclic electron transport (CET) around PS1. The ratio of the amplitudes of EPR signals from <span>({text{P}}_{{700}}^{ + })</span> under the white and far-red light (707 nm) became higher in plants grown under strong light. This might be due to an increase in CET, which helped to optimize the energy balance and reduce light stress when there was an excess of illumination. The results obtained are discussed in the context of the task of optimizing photosynthetic processes during plant acclimation.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"92 - 105"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction of Mesenchymal Stromal Cells with 5XFAD Mouse Hippocampal Cells in Primary Culture Depending on Cocultivation Method","authors":"A. V. Chaplygina,&nbsp;D. Y. Zhdanova,&nbsp;V. I. Kovalev,&nbsp;R. A. Poltavtseva,&nbsp;N. V. Bobkova","doi":"10.1134/S1990747823030042","DOIUrl":"10.1134/S1990747823030042","url":null,"abstract":"<p>We studied the mutual influence of multipotent mesenchymal stromal cells (MMSC) isolated from human umbilical cord Wharton’s jelly and primary culture of hippocampal cell from transgenic (Tg) 5XFAD mice, a model of familial Alzheimer’s disease (AD). Antibodies to human nuclear antigen were used to identify MMSCs in “chimeric culture”; the cells belonging to neurons or astrocytes were determined by the presence of positive immunoreactivity to marker proteins MAP2 and GFAP. It was shown that the result of the interaction depends on both the cocultivation method and the age of the culture. In indirect (non-contact) cocultivation, the aggressive environment of the transgenic culture affected the survival rate and impaired the adhesive properties of the MMSCs. Pretreatment of these cells with stress proteins YB-1 and HSP70, which possess neuroprotective properties, increased the resistance of MMSCs. In young culture during contact cocultivation, the MMSCs play the role of specific strands that promote grouping of hippocampal cells of transgenic mice and formation of neurospheres. In old transgenic cultures, irrespective of the cocultivation method, the MMSCs differentiated into astrocytes, but during prolonged direct cocultivation, a part of MMSCs became immunopositive to the neuronal marker MAP2. The work shows that the interaction between the MMSCs and the hippocampal cell culture can be carried out with the participation of gap junctions as well as due to the formation of nanotubes. The results obtained indicate the presence of a complex relationship between donor MMSCs and recipient cells, which must be taken into account when introducing cell therapy into the practice of treating AD patients.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"156 - 168"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BAR Domain Proteins as Putative Regulators of the Protein Liquid Phase in Nerve Terminals in the Central Nervous System","authors":"A. G. Shishkov,&nbsp;N. V. Nifantova,&nbsp;O. M. Korenkova,&nbsp;E. S. Sopova,&nbsp;L. Brodin,&nbsp;O. Shupliakov","doi":"10.1134/S199074782303008X","DOIUrl":"10.1134/S199074782303008X","url":null,"abstract":"<p>BAR proteins are key components of the synaptic vesicle cycle in nerve terminals. They participate in the regulation of neurotransmitter release during the fusion of synaptic vesicles with the presynaptic membrane and synaptic vesicle recycling. Localization of these proteins at the sites of liquid–liquid phase separation in nerve terminals suggests additional functions of these molecules. In the current review, we discuss the tasks of BAR proteins at different stages of the secretory cycle, including their putative role in liquid–liquid phase transitions in nerve terminals during synaptic activity. We suggest that BAR proteins, along with their established functions in exo- and endocytosis, play crucial roles in the organization of the reserve pool of synaptic vesicles and at intermediate stages of the vesicle cycle.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"69 - 82"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic Lipoaminoacid Derivatives of Diethanolamine As Potentially Membrane-Active Antibacterial Agents","authors":"M. K. Guseva,&nbsp;Z. G. Denieva,&nbsp;U. A. Budanova,&nbsp;Yu. L. Sebyakin","doi":"10.1134/S1990747823020034","DOIUrl":"10.1134/S1990747823020034","url":null,"abstract":"<p>This work is aimed to develop several cationic amphiphiles based on amino acid derivatives of diethanolamine as potentially membrane-active antibacterial agents. The developed compounds contain two amino acid residues in the polar block and aliphatic chains of various length in the hydrophobic domain. Amphiphiles were obtained in preparative amounts sufficient to confirm their structures and perform a study of antibacterial activity. The synthesized samples based on β-Ala <b>(4c)</b> and GABA <b>(4d)</b> with aliphatic C12 chain in the hydrophobic domain showed a promising level of antimicrobial activity against gram-positive (<i>B. subtilis</i>) and gram-negative (<i>E. coli</i>) bacteria (minimal inhibitory concentration, MIC, 1 μg/mL). Amphiphiles containing aromatic amino acids <i>L</i>-Phe <b>(6a)</b> and <i>L</i>-Trp <b>(6b)</b> in the polar head group and C8 hydrocarbon chain exhibited an antibacterial activity against <i>B. subtilis</i> with MIC of 1 μg/mL. The obtained data on antimicrobial activity make the selected compounds attractive for further detailed study of their mechanism of action.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 2","pages":"148 - 155"},"PeriodicalIF":0.5,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4725045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV-2 Receptors and Their Involvement in Cell Infection","authors":"P. P. Avdonin,&nbsp;E. Yu. Rybakova,&nbsp;S. K. Trufanov,&nbsp;P. V. Avdonin","doi":"10.1134/S1990747822060034","DOIUrl":"10.1134/S1990747822060034","url":null,"abstract":"<p>The new coronavirus infection (COVID-19) pandemic caused by SARS-CoV-2 has many times surpassed the epidemics caused by SARS-CoV and MERS-CoV. The reason for this was the presence of sites in the protein sequence of SARS-CoV-2 that provide interaction with a broader range of receptor proteins on the host cell surface. In this review, we consider both already known receptors common to SARS-CoV and SARS-CoV-2 and new receptors specific to SARS-CoV-2.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"1 - 11"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1990747822060034.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5121241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Perfluorocarbon Nanoparticles on Blood As a Cellular System","authors":"I. N. Kuznetsova,&nbsp;A. Yu. Lyanguzov","doi":"10.1134/S1990747823010051","DOIUrl":"10.1134/S1990747823010051","url":null,"abstract":"<div><div><h3>\u0000 <b>Abstract</b>—</h3><p>The effect of perfluorocarbon (PFC) nanoparticles on the functioning of blood cells is considered. Evidence is presented indicating that emulsified PFC nanoparticles in small volumes affect gas transport function of blood, increasing the rate of oxygen diffusion from erythrocytes to tissues. It was found that PFC nanoparticles also affect the state of blood cells (erythrocytes, leukocytes, and platelets) in a dose-dependent manner. It was assumed that depending on the emulsion dose, that is, the density of nanoparticles in the surrounding space of cells, the reaction of receptor apparatus can cause activation or deterioration of their functioning.</p></div></div>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":"17 1","pages":"20 - 27"},"PeriodicalIF":0.5,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5127767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}