Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2024-06-25 DOI:10.1134/S1990747824700120

L. V. Dudareva, E. G. Rudikovskaya, N. V. Semenova, A. V. Rudikovskii, V. N. Shmakov

{"title":"Influence of Low-Intensive He-Ne Laser Radiation on the Composition and Content of Phospholipids and Sterols in the Callus Tissues of Wheat Тriticum aestivum L.","authors":"L. V. Dudareva, E. G. Rudikovskaya, N. V. Semenova, A. V. Rudikovskii, V. N. Shmakov","doi":"10.1134/S1990747824700120","DOIUrl":null,"url":null,"abstract":"<p>The effect of He-Ne laser radiation on the composition and content of cell membrane components, namely phospholipids (PL) and sterols, in wheat callus tissues was studied by chromato-mass spectrometry and thin-layer chromatography. It was shown that irradiation of calluses with laser light at a dose of 3.6 J/cm<sup>2</sup> resulted in significant changes in the content of these components. Thus, the content of phosphatidylinositol increased in irradiated callus by 8 times, phosphatidylethonolamine by 2 times, and the content of phosphatidic acid decreased by 20% of the sum of PL. For sterols it was found that irradiation caused the most significant changes in the content of β-sitosterol dominant in plants (increase from 1453 ± 170 μg/g dry weight in unirradiated control to 2001 ± 111 μg/g dry weight 1 h after exposure); due to this, the total content of sterols also increased. The analysis of the obtained results suggests that PLs and sterols, primarily those for which regulatory and signaling functions are known, participate in the reaction of plant tissue to exposure to low-intensity He-Ne laser irradiation. This participation is realized as a stress (nonspecific) response to intense radiation.</p>","PeriodicalId":484,"journal":{"name":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","volume":null,"pages":null},"PeriodicalIF":1.1000,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology","FirstCategoryId":"2","ListUrlMain":"https://link.springer.com/article/10.1134/S1990747824700120","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The effect of He-Ne laser radiation on the composition and content of cell membrane components, namely phospholipids (PL) and sterols, in wheat callus tissues was studied by chromato-mass spectrometry and thin-layer chromatography. It was shown that irradiation of calluses with laser light at a dose of 3.6 J/cm² resulted in significant changes in the content of these components. Thus, the content of phosphatidylinositol increased in irradiated callus by 8 times, phosphatidylethonolamine by 2 times, and the content of phosphatidic acid decreased by 20% of the sum of PL. For sterols it was found that irradiation caused the most significant changes in the content of β-sitosterol dominant in plants (increase from 1453 ± 170 μg/g dry weight in unirradiated control to 2001 ± 111 μg/g dry weight 1 h after exposure); due to this, the total content of sterols also increased. The analysis of the obtained results suggests that PLs and sterols, primarily those for which regulatory and signaling functions are known, participate in the reaction of plant tissue to exposure to low-intensity He-Ne laser irradiation. This participation is realized as a stress (nonspecific) response to intense radiation.

Abstract Image

查看原文本刊更多论文

低强度氦氖激光辐射对小麦胼胝体组织中磷脂和甾醇成分及含量的影响

摘要利用色质谱法和薄层色谱法研究了氦氖激光辐射对小麦胼胝体组织中细胞膜成分（即磷脂和甾醇）的组成和含量的影响。结果表明，用剂量为 3.6 J/cm2 的激光照射胼胝体，会导致这些成分的含量发生显著变化。因此，辐照胼胝体中磷脂酰肌醇的含量增加了 8 倍，磷脂酰肌醇胺的含量增加了 2 倍，而磷脂酸的含量则减少了磷脂酰肌醇总和的 20%。在甾醇方面，辐照对植物中占优势的 β-谷甾醇含量的变化最为显著（从未受理辐照对照的 1453 ± 170 μg/g 干重增加到照射 1 小时后的 2001 ± 111 μg/g 干重）；因此，甾醇的总含量也有所增加。对所得结果的分析表明，聚乳酸和甾醇（主要是那些已知具有调节和信号功能的物质）参与了植物组织对低强度氦氖激光照射的反应。这种参与是作为对强辐射的应激（非特异性）反应实现的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.