Corneal Endothelial Cell Volume Regulation Disorders in Keratoconus

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2024-04-01 DOI:10.1134/S1990747824700065

I. M. Kuseina, L. E. Katkova, G. S. Baturina, I. G. Palchikova, I. A. Iskakov, E. I. Solenov

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Abstract

The study of the permeability to water and urea of plasma membranes of endothelial cells of the normal cornea and cornea with a pronounced form of keratoconus was carried out. Human corneal endothelial cells were obtained from surgical material. Determination of osmotic water permeability (P_f) of normal and keratoconus endothelial cells did not reveal significant differences in the value of this parameter in both groups, where cells had a similar osmotic water permeability (control P_f = 0.53 ± 0.045 cm/s; keratoconus P_f = 0.63 ± 0.041 cm/s) (n = 25; p ≥ 0.05). The urea permeability coefficients (P_u) in both groups also had no statistically significant differences (control P_u = 0.049 ± 0.003 cm/s; keratoconus P_u = 0.056 ± 0.003 cm/s) (n = 25; p ≥ 0.05). Analysis of cell volume dynamics based on exponential approximation showed that corneal endothelial cells with keratoconus reduced their volume in a hypertonic medium to a greater extent compared to cells of a healthy cornea. An increase in cell volume as a result of isotonic urea inflow in a hypertonic medium also occurred to significantly higher values compared to normal cells. The conclusion was made about significant changes in the mechanism of regulation of the volume of corneal endothelial cells in keratoconus.

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角膜炎患者的角膜内皮细胞体积调节障碍

摘要研究了正常角膜和明显角膜炎角膜内皮细胞质膜对水和尿素的通透性。人类角膜内皮细胞取自手术材料。测定正常角膜内皮细胞和角膜内皮细胞的渗透水渗透性（Pf），结果显示两组细胞的渗透水渗透性（对照组 Pf = 0.53 ± 0.045 cm/s；角膜病变组 Pf = 0.63 ± 0.041 cm/s）相似（n = 25；p ≥ 0.05），该参数值无显著差异。两组的尿素渗透系数（Pu）差异也无统计学意义（对照组 Pu = 0.049 ± 0.003 cm/s；角膜病 Pu = 0.056 ± 0.003 cm/s）（n = 25；p ≥ 0.05）。基于指数近似法的细胞体积动态分析显示，与健康角膜细胞相比，患有角膜炎的角膜内皮细胞在高渗介质中的体积减少程度更大。与正常细胞相比，高渗培养基中等渗尿素流入导致的细胞体积增加值也明显更高。由此得出结论，角膜炎患者角膜内皮细胞体积的调节机制发生了重大变化。

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.