{"title":"A maxillary location of extra-nodal Rosai Dorfman disease: a case report.","authors":"Yassine Bennaoui, Mohamed Salah Koussay Hattab, Fadoua Fettal, Zakaria Aziz, Hanane Rais, Nadia Mansouri Hattab","doi":"10.11604/pamj.2024.48.20.43457","DOIUrl":"10.11604/pamj.2024.48.20.43457","url":null,"abstract":"<p><p><b>Introduction:</b> Rosai-Dorfman disease (RDD) is a rare benign histiocytic proliferation, characterized by a group of clinical symptoms. This report presents a case of extranodal RDD manifesting as a progressively enlarging left maxillary mass in a 42-year-old woman. Surgical exploration and biopsy confirmed the diagnosis of RDD, with characteristic histopathological features including emperipolesis. Treatment involved corticotherapy, resulting in controlled maxillary pain and improvement of the disease after one year. This case underscores the potential for extra-nodal RDD presentations, posing diagnostic challenges and emphasizing the importance of considering RDD in the differential diagnosis of maxillary masses.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pan African Medical JournalPub Date : 2024-05-24eCollection Date: 2024-01-01DOI: 10.11604/pamj.2024.48.19.43659
Ernest Kateule, Oscar Nzila, William Ngosa, Fred Mfume, Chola Shimangwala, Angela Gama, Sophia Msiska
{"title":"Multisectoral approach for the control of cholera outbreak - lessons and challenges from Lusaka district - Zambia, October 2023 - February 2024.","authors":"Ernest Kateule, Oscar Nzila, William Ngosa, Fred Mfume, Chola Shimangwala, Angela Gama, Sophia Msiska","doi":"10.11604/pamj.2024.48.19.43659","DOIUrl":"10.11604/pamj.2024.48.19.43659","url":null,"abstract":"<p><strong>Introduction: </strong>on October 18, 2023, the Ministry of Health declared an outbreak of cholera in the Lusaka district. Public health interventions were implemented using a multisectoral approach in the Lusaka district and other hotspots in the country. We documented the multisectoral response efforts and their impacts on the cholera epidemic in the Lusaka district of Zambia. We highlighted the major challenges and their associated impacts on the epidemiologic patterns of disease in hotspot areas.</p><p><strong>Methods: </strong>we conducted a descriptive observational study of cholera response activities in the Lusaka district. We used quantitative and qualitative non-participant techniques using the Centers for Disease Prevention and Control's direct in-person observation tool in healthcare settings. We reviewed surveillance records to estimate the magnitude of the outbreak, and characterized cases by person, place, and time. We documented the response interventions and challenges using situation reports.</p><p><strong>Results: </strong>during the 2023 - 2024 cholera outbreak, Lusaka district was the most affected district with 13,122 cases and 498 deaths as of 12<sup>th</sup> February 2024. Despite having a well-established system for coordinating technical support and resource mobilization, inadequate sanitation and limited access to clean water remained potential risks for cholera outbreaks in Lusaka district.</p><p><strong>Conclusion: </strong>Lusaka district may have experienced one of the most severe cholera epidemics in the nation's history, as indicated by its rapid spread and increased mortality reported from both the community and treatment centers. A multisectoral coordination for improved sanitary systems, access to clean water, health education strategies, and vaccination campaigns contributed to the decline in cholera cases.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Epidemiological profile of patients attending the maxillofacial prosthodontics unit at Ibn Sina University Hospital in Rabat, Morocco: a cross-sectional study.","authors":"Mohamed Azhari, Abdoulmajid Habibou, Oussama Bentahar","doi":"10.11604/pamj.2024.48.21.40925","DOIUrl":"10.11604/pamj.2024.48.21.40925","url":null,"abstract":"<p><strong>Introduction: </strong>the present study aimed to establish an epidemiological profile of patients consulting the unit of maxillofacial prosthodontics in Rabat. Results deriving from this study will help enhance the quality of patient care in our center and can also serve as a comparison tool with other maxillofacial teams around the world.</p><p><strong>Methods: </strong>during 11 months all patients consulting the unit of maxillofacial prosthodontics in our center were included. We opted for a questionnaire with 3 variables: socio-demographics, clinical examination data, and the type of prosthetic treatment adopted. The study was conducted in the Department of Removable Prosthodontics in the Center of Consultation and Dental Treatment of Rabat Morocco (CCDTR) from September 2020 to July 2021.</p><p><strong>Results: </strong>the study population consisted of 91 patients, with a majority of male patients at 53% (n=48). During our study period, the proportion of patients under one-year-old was predominant, accounting for 38.4% (n=35) of the total sample. Among the patients in the sample, 72.5% (n=66) had no profession, while 66% (n=60) had a low income. Regarding medical insurance, the majority of our patients, 85.5% (n=78), were covered by Public Health Insurance for the Low-income Population (PHILP). Among the total sample, 57.1% (n=52) consulted for a combination of pain function and aesthetics. Additionally, 61.5% (n=56) were referred by teaching hospitals. For the type of oro-facial defect, 52.7% (n=48) of the sample consisted of newborns with congenital facial cleft. Out of the 91 patients, 36 had maxillofacial tumors, with 47.6% (n=17) of them presenting squamous cell carcinoma. Furthermore, 63.7% (n=58) of the prosthetic treatments adopted involved presurgical orthopedic treatments for newborns with facial cleft.</p><p><strong>Conclusion: </strong>the study on the epidemiological profile of patients attending the maxillofacial prosthodontics unit at Ibn Sina University Hospital in Rabat, Morocco provides important insights. The findings highlight the predominance of male patients and the prevalence of oro-facial defects in newborns. Socioeconomic factors, such as low income and lack of profession, are significant considerations. The majority of patients are covered by the PHILP, indicating the importance of medical insurance. These findings contribute to improving healthcare planning and specialized care for this patient population.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11364889/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142113457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Intravenous versus intracuff alkalinized lidocaine to prevent postoperative sore throat: a prospective randomized controlled trial.","authors":"Salma Ketata, Yassine Maktouf, Imen Zouche, Sarhane Feki, Mariem Keskes, Ayman Trigui, Amira Akrout, Rahma Daoud, Amine Chaabouni, Hichem Cheikhrouhou","doi":"10.11604/pamj.2024.48.18.40317","DOIUrl":"10.11604/pamj.2024.48.18.40317","url":null,"abstract":"<p><strong>Introduction: </strong>postoperative sore throat (POST) is a common complication after general anesthesia with endotracheal intubation caused by tracheal mucosal injury. Multiple techniques prevent postoperative sore throat (POST). Our study aimed to compare two techniques: intravenous and intracuff lidocaine versus placebo to prevent postoperative sore throat after general anesthesia with orotracheal intubation.</p><p><strong>Methods: </strong>we conducted a prospective double-blind, randomized controlled clinical trial involving patients, proposed for a scheduled surgery less than 240 minutes under general anesthesia with orotracheal intubation. Patients were divided into three groups: L group: infused with saline, cuff filled with alkalinized lidocaine. S group: Infused with 1.5 mg/kg of lidocaine, cuff filled with saline. T group: placebo: infused with saline, cuff filled with saline. Our primary outcome was the incidence of sore throat and their (visual analog scale) VAS score in the first 24 postoperative hours. Our secondary outcomes were the incidence of cough, dysphonia, dysphagia, and postoperative nausea and vomiting.</p><p><strong>Results: </strong>ninety patients were analyzed and divided into 3 groups of 30. The incidence of POST at the sixth postoperative hour, for placebo, the \"L\" group, and the \"S\" group, respectively, was 67%, 30%, and 47%. And at the 24<sup>th</sup> postoperative hours 67%, 13%, and 37%. Intravenous lidocaine reduced significantly the VAS of POST at the 24<sup>th</sup> hour (S: 6.80 ± 20.70; T: 20.67 ± 18.182; p= 0.02). Alkalinized lidocaine decreased significantly the VAS of POST in the sixth (L: 8.17 ± 22.761; T: 23 ± 21.838; p = 0.048) and the 24<sup>th</sup> postoperative hour (L: 6.33 ± 20.592; T: 20.67 ± 18.182; p= 0.019) with the lowest pain score. There was no statistically significant difference between the L and S groups at the 6 and 24 postoperative hours. Both lidocaine techniques reduced cough at emergence, with the superiority of alkalinized lidocaine (p=0.02). They decreased the incidence of cough, dysphonia, dysphagia, nausea, and vomiting compared to a placebo.</p><p><strong>Conclusion: </strong>intravenous and intracuff lidocaine allowed better control of postoperative sore throat.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pan African Medical JournalPub Date : 2024-05-21eCollection Date: 2024-01-01DOI: 10.11604/pamj.2024.48.17.42481
Idriss Ziani, Ahmed Ibrahimi
{"title":"[Ureterohydronephrosis with rupture of the fornix due to pyelo-ureteral junction syndrome].","authors":"Idriss Ziani, Ahmed Ibrahimi","doi":"10.11604/pamj.2024.48.17.42481","DOIUrl":"10.11604/pamj.2024.48.17.42481","url":null,"abstract":"","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pan African Medical JournalPub Date : 2024-05-20eCollection Date: 2024-01-01DOI: 10.11604/pamj.2024.48.15.42881
Amina Chrifi Alaoui, Mohammed Omari, Noura Quarmich, Omar Kouiri, Basmat Amal Chouhani, Mohammed Berraho, Nabil Tachfouti, Tarik Sqalli Houssaini, Samira El Fakir
{"title":"Prevalence and determinant factors of depression and anxiety in people with chronic kidney disease: a Moroccan cross-sectional study.","authors":"Amina Chrifi Alaoui, Mohammed Omari, Noura Quarmich, Omar Kouiri, Basmat Amal Chouhani, Mohammed Berraho, Nabil Tachfouti, Tarik Sqalli Houssaini, Samira El Fakir","doi":"10.11604/pamj.2024.48.15.42881","DOIUrl":"10.11604/pamj.2024.48.15.42881","url":null,"abstract":"<p><strong>Introduction: </strong>the chronic kidney disease (CKD), is associated with various psychiatric conditions and poorer quality of life. The main objective of this study is to estimate the prevalence of depression and anxiety among CKD patients and to assess their determinant factors.</p><p><strong>Methods: </strong>this is a cross-sectional study in a Moroccan university hospital. Patients aged ≥18 years and followed for more than one year for a stage 3 to 5 CKD, not under renal replacement therapy (RRT) were included. The data were collected using a questionnaire and the hospital anxiety and depression scale (HADS). The association between depression and anxiety sub-scores and the estimated glomerular filtration rate (eGFR) was assessed using simple and multiple linear regression.</p><p><strong>Results: </strong>eighty-three patients (mean age 61.7 ± 14.1 years, and 61.4% were women), were included. Regarding the stages of CKD, 10.7% were in stage 3, 52.2% in stage 4, and 25% in stage 5 of CKD. The median of the depression sub-score was 5.00 (IQR (2.00; 10.0)). The median of the depression sub-score was 5.00 (IQR (2.00; 10.09)), and the median of the anxiety sub-score was 6.00 (IQR (4.00; 9.00)). It also shows that 22.0% of patients had depression (stage 4 = 23.8%, stage 5 = 36.8%) and 22.2% had anxiety (stage 4 = 26.2%, stage 5 = 33.3%). Both depression and anxiety scores were significantly associated with the eGFR before (p<0.001, p= 0.001 respectively) and after adjustment (p=0.001, p=0.002 respectively).</p><p><strong>Conclusion: </strong>according to this study depression and anxiety are strongly related to eGFR.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of stage-matched educational intervention on use of institutional delivery in Northwest Ethiopia: using community readiness model.","authors":"Adane Nigusie, Telake Azale, Mezgebu Yitayal, Lemma Derseh","doi":"10.11604/pamj.2024.48.16.37504","DOIUrl":"10.11604/pamj.2024.48.16.37504","url":null,"abstract":"<p><strong>Introduction: </strong>even though there are many initiatives to improve institutional delivery, there are low service utilization and community readiness for institutional delivery in Ethiopia. This study assessed the role of community readiness on delivery service use.</p><p><strong>Methods: </strong>a pre-and post-test design with a control group was used for the evaluation of the stage-matched educational intervention following the protocol of the community readiness assessment model. Based on the baseline assessment of community readiness among 15 kebeles where the study was conducted, the overall score of nine kebeles was below stage-5 out of the nine stages, which were targeted for the intervention. The intervention group (n= three kebeles) participated in the stage-matched intervention for 15 months, while the control group (n= three kebeles) were not given the intervention. The data were analyzed using the difference in difference (DiD) method.</p><p><strong>Results: </strong>there were significant improvements in a stage of change for the promotion of institutional delivery (p-value <0.001) and institutional delivery use (p-value <0.001) in the intervention group as compared to the control group. The study revealed that the intervention influenced community resource allocation (at marginally significant levels), improved leader-ship quality of prevention, and community climate to supportive prevention efforts. There was evidence that the intervention (health promotion) also increased service use at a significant level.</p><p><strong>Conclusion: </strong>the community readiness-based intervention (health promotion) can be useful to measure the combined attitude and behavior towards institutional delivery services. The village-based mobilizer approach had a positive effect on institutional delivery use and the level of community readiness on the promotion of institutional delivery.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pan African Medical JournalPub Date : 2024-05-16eCollection Date: 2024-01-01DOI: 10.11604/pamj.2024.48.14.42194
Immaculate Wambui Kamau, Margaret Nyanchoka Keraka, Eliphas Gitonga
{"title":"Effect of post-discharge postnatal educational intervention on postnatal practices among low-income primiparas in Nairobi informal settlements, Kenya: a post-test quasi-experiment.","authors":"Immaculate Wambui Kamau, Margaret Nyanchoka Keraka, Eliphas Gitonga","doi":"10.11604/pamj.2024.48.14.42194","DOIUrl":"10.11604/pamj.2024.48.14.42194","url":null,"abstract":"<p><strong>Introduction: </strong>informal settlements exhibit disproportionately worse maternal and newborn outcomes. Postnatal care (PNC) is a high-impact intervention for populations contributing to higher mortalities. Postnatal education is crucial to adopting evidence-based postnatal practices (PNPs) thus its post-discharge reinforcement is vital for low-income primiparas. This study aimed to determine the effect of post-discharge follow-up postnatal education intervention (PNE) on the adoption of recommended PNPs among low-income primiparas.</p><p><strong>Methods: </strong>quasi-experimental study in Nairobi informal settlements with 118 primiparas discharged early after normal delivery on each arm. Facility and demographic data collected using an interviewer-administered questionnaire. Study arm received the intervention for 6 weeks in addition to routine PNC while control received routine PNC only. An interviewer-administered exit questionnaire was applied after 6 weeks. Focus group discussions were conducted for qualitative data then analyzed thematically. SPSS used to analyze quantitative data then descriptive statistics, t-tests, Chi-square, Mann-Whitney, and multiple linear or logistic regressions derived. PNPs composed of health-seeking for maternal and newborn danger signs, self and baby care practices, and utilization of PNC contacts.</p><p><strong>Results: </strong>the intervention was a positive predictor of adoption of composite PNPs (β=0.26, p=0.00), self-care practices (β=0.39, p=0.00) and mothers' two weeks PNC contact (OR=4.64, p=0.00, 95% CI=1.9-11.2). Neither a significant predictor of health-seeking for maternal (β=-0.11, p=0.31) nor newborns danger signs (β=-0.04, p=0.73) though inversely related. No influence on baby care practices, two weeks of newborn contact and six weeks contact for the dyad. Multi-pronged approaches are appreciated.</p><p><strong>Conclusion: </strong>follow-up post-discharge PNE intervention enhances adoption of PNPs among low-income primiparas thus a worthwhile supplement to routine PNC.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pan African Medical JournalPub Date : 2024-05-15eCollection Date: 2024-01-01DOI: 10.11604/pamj.2024.48.13.43454
Ni Putu Ayu Reza Dhiyantari, Delfitri Lutfi, Dwi Hari Susilo, Irwan Kristyono, Alicia Widya
{"title":"A rare presentation of rhino-orbital-cutaneous mucormycosis in an immunocompetent patient: a case report.","authors":"Ni Putu Ayu Reza Dhiyantari, Delfitri Lutfi, Dwi Hari Susilo, Irwan Kristyono, Alicia Widya","doi":"10.11604/pamj.2024.48.13.43454","DOIUrl":"10.11604/pamj.2024.48.13.43454","url":null,"abstract":"<p><p>Mucormycosis is a rare opportunistic infection caused by Mucorales fungi. Cutaneous mucormycosis typically present as chronic indolent infection, whereas rhino-orbital mucormycosis is rapidly progressive disease often invade the adjacent cerebral tissue associated with high mortality. This case represents the atypical clinical history of rhino-orbital-cutaneous mucormycosis. The patient was presented with a right orbital cellulitis associated with an extensive multiple suppurative deep cutaneous infection and worsening headache. The skin lesion was initiated from a localized abscess at the right periorbital area nine months before admission. Suspicion of fungal infection was raised after weeks of non-responsive antibiotics treatment. Aggressive treatment with exoneration of the right eye and surgical debridement was undertaken. Periodic acid Schiff staining from healthy periorbital tissue revealed ribbon-like hyphae with pauciseptate and 90° branching identified as Mucoraceaefamily. The resolution was seen after four weeks of antifungal treatment with Amphotericin B.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pan African Medical JournalPub Date : 2024-05-14eCollection Date: 2024-01-01DOI: 10.11604/pamj.2024.48.12.33596
Albertina Mariina Ndinelao Shatri
{"title":"Biochemical characterization of actinomycete from Namibia rocky crest mountainous soil and analyzing their bioactive metabolites for antagonistic effect against human respiratory pathogens.","authors":"Albertina Mariina Ndinelao Shatri","doi":"10.11604/pamj.2024.48.12.33596","DOIUrl":"10.11604/pamj.2024.48.12.33596","url":null,"abstract":"<p><strong>Introduction: </strong>the present study aimed at isolating and characterizing actinomycete from unexplored Windhoek rocky crest mountainous soil and extracting bioactive metabolites as possible therapeutics against common life-threatening Streptococcus pneumonia (S. pneumonia) and Stachybotrys chartarum (S. chartarum).</p><p><strong>Methods: </strong>chemotaxonomy and biochemical methods were used to identify the isolates. The solvent extraction method was used to extract bioactive compounds. Agar overlay and disc diffusion methods were used to determine the antimicrobial activity of isolates and extracted bioactive metabolites against S. pneumonia and S. chartarum. The antioxidant activity of the extracted bioactive metabolites was determined using 2.2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method with ascorbic acid as a positive control. Comparison between groups was done using a Two-way ANOVA, followed by Bonferroni post-test.</p><p><strong>Results: </strong>three distinct isolates from 3 soil samples were identified on starch casein agar and distinguished using biochemical tests. All three isolates showed strong inhibitory activity against S. pneumonia with average growth inhibition zones between 18.0±1.00 and 27±0.00 mm p< 0.005. All isolates showed potent inhibitory activity against S. chartarum with the average inhibition zones ranging between 42.0±1.00 and 48±0.00 mm, p< 0.005. The chloroform extracts showed potent DPPH activity of up to 73± 1.41%.</p><p><strong>Conclusion: </strong>growth conditions and extraction solvents can influence the antimicrobial and antioxidant properties of bioactive metabolites.</p>","PeriodicalId":48190,"journal":{"name":"Pan African Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.9,"publicationDate":"2024-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142056936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}