Journal of Natural Products 最新文献_第9页

Discovery of Dual-Target Anti-Alzheimer’s Filicinic Acid-Based Meroterpenoids from Hypericum japonicum Thunb. 从日本金丝桃中发现双靶点抗阿尔茨海默病的菲利酸基美罗萜类化合物。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-04-17 DOI: 10.1021/acs.jnatprod.6c00131

Shengfei Zhong, Shoulun He, Junjie Chen, Jingyu Sun, Lulu Fu, Yingting Lin, Yuhang You, Guanghui Wang, Xiaobin Li*, Haifeng Chen* and Wenjing Tian*,

{"title":"Discovery of Dual-Target Anti-Alzheimer’s Filicinic Acid-Based Meroterpenoids from Hypericum japonicum Thunb.","authors":"Shengfei Zhong, Shoulun He, Junjie Chen, Jingyu Sun, Lulu Fu, Yingting Lin, Yuhang You, Guanghui Wang, Xiaobin Li*, Haifeng Chen* and Wenjing Tian*, ","doi":"10.1021/acs.jnatprod.6c00131","DOIUrl":"10.1021/acs.jnatprod.6c00131","url":null,"abstract":"Seven undescribed filicinic acid-based meroterpenoids, hyperjaponiones A–G (1–7), along with a new natural product (hyperjaponione H, 8) and 10 known analogues (9–18), were isolated from Hypericum japonicum Thunb. Chiral separation of compound 1 afforded a pair of racemates (±)-1 (1a and 1b). Their structures were elucidated through a comprehensive spectroscopic analysis and quantum chemical calculations. Moreover, biological evaluation revealed that 8 activated peroxisome proliferator-activated receptor-γ (PPARγ) transcription and upregulated the level of ATP-binding cassette transporter A1 (ABCA1), showing promise in promoting β-amyloid (Aβ) clearance. Surface plasmon resonance analysis indicated that 8 might interacted with the PPARγ ligand-binding domain, with an estimated binding affinity (Kd) of 5.63 μM. Molecular docking study further predicted the binding mode of 8 to PPARγ. Additionally, 8 was found to inhibit β-site amyloid precursor protein cleaving enzyme 1 (BACE1) activity, showing potential in reducing Aβ generation. In an in vivo zebrafish model, 8 exhibited notable anti-Alzheimer’s disease effects with no detectable toxicity or teratogenicity. Thus, 8 acts as a dual-target agent by both activating PPARγ and inhibiting BACE1, highlighting its potential as a novel therapeutic candidate for Alzheimer’s disease.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1711–1723"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147715344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Derbesiamides A–E, Cyclic Depsipeptides from Pedobesia sp. Derbesiamides A-E，来自Pedobesia sp.的环状沉积肽。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-18 DOI: 10.1021/acs.jnatprod.6c00400

Towa Tsukamoto, Yuki Yamazaki, Takeaki Hanyuda, Kazutoshi Yoshitake and Kentaro Takada*,

引用次数: 0

Editorial Introducing the Collection in Honor of Valerie J. Paul 社论介绍了瓦莱丽J.保罗的荣誉收藏

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 DOI: 10.1021/acs.jnatprod.6c00688

Hendrik Luesch*, and , William Fenical*,

引用次数: 0

Elesestriterpenoid A, an Unprecedented 5/7/6/6/5 Carbon Skeleton with Neuroprotective Activity from Leaves of Eleutherococcus sessiliflorus 一种前所未有的、具有神经保护作用的棘球绦虫叶片碳骨架——棘球绦虫萜类A。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-08 DOI: 10.1021/acs.jnatprod.6c00058

Yi-Qiang Zhang, Dong Han, Rui Yang, Shu-Jun Zhang, Yan-Ying Li, Lei-Xin Zhuang, Ke-Zhen Chen, Jia-Tong Wu, Juan Pan, Wei Guan, Meng-Meng Li, Qing-Shan Chen, Li−Li Zhang, Hai-Xue Kuang and Yan Liu*,

{"title":"Elesestriterpenoid A, an Unprecedented 5/7/6/6/5 Carbon Skeleton with Neuroprotective Activity from Leaves of Eleutherococcus sessiliflorus","authors":"Yi-Qiang Zhang, Dong Han, Rui Yang, Shu-Jun Zhang, Yan-Ying Li, Lei-Xin Zhuang, Ke-Zhen Chen, Jia-Tong Wu, Juan Pan, Wei Guan, Meng-Meng Li, Qing-Shan Chen, Li−Li Zhang, Hai-Xue Kuang and Yan Liu*, ","doi":"10.1021/acs.jnatprod.6c00058","DOIUrl":"10.1021/acs.jnatprod.6c00058","url":null,"abstract":"One undescribed triterpenoid and four novel triterpenoid saponins, elesestriterpenoids A–E (1–5) were isolated from the leaves of Eleutherococcus sessiliflorus (Rupr. & Maxim.) S. Y. Hu. Their chemical structures were determined using spectroscopic data analysis, with their corresponding absolute configurations measured using single-crystal X-ray diffraction, electronic circular dichroism calculations, and DP4+ technologies. Notably, elesestriterpenoid A (1) represents the first reported lupane-type triterpenoid with a 5/7/6/6/5-fused ring system from nature, and elesestriterpenoids B-E (2–5) feature an unprecedented 7/6/6/5-fused carbon skeleton. The neuroprotective potential of these isolates was evaluated using an in vitro model of oxidative stress in l-glutamate-induced HT22 mouse hippocampal neuron cells. The results revealed significant neuroprotective activity for compounds 1, 3, and 4. Particularly noteworthy, compound 1 demonstrated pronounced efficacy in mitigating oxidative damage through modulation of the Keap1/Nrf2/HO-1 signaling pathway in HT22 cells, indicating its potential for treating nerve injuries.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1671–1685"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activation of a Silent Type II PKS Pathway in Streptomyces avermitilis via a Reintroduction Strategy 通过重新引入策略激活阿维链霉菌沉默的II型PKS通路

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-06-09 DOI: 10.1021/acs.jnatprod.6c00382

Tengfei Zhao, Zengguang Zhang, Jinfen Yuan, Shaofei Wang, Guangtao Zhang* and Chunshuai Huang*,

{"title":"Activation of a Silent Type II PKS Pathway in Streptomyces avermitilis via a Reintroduction Strategy","authors":"Tengfei Zhao, Zengguang Zhang, Jinfen Yuan, Shaofei Wang, Guangtao Zhang* and Chunshuai Huang*, ","doi":"10.1021/acs.jnatprod.6c00382","DOIUrl":"10.1021/acs.jnatprod.6c00382","url":null,"abstract":"Genome mining of the industrially important bacterium Streptomyces avermitilis was performed via antiSMASH prediction and Protein BLAST analysis, which uncovered a previously uncharacterized cryptic type II polyketide synthase (PKS) biosynthetic gene cluster (BGC) designated spy. To activate this silent biosynthetic pathway in its native genomic background, we employed a gene cluster reintroduction strategy in which the spy cluster was captured and reintroduced into the optimized chassis strain S. avermitilis SUKA17. This approach successfully activated the silent pathway, leading to the isolation and characterization of six new griseusin analogues, spiromycins A–F (1–6), together with two anthraquinone derivatives, spoanthraquinones A (7) and B (8). Bioactivity evaluation revealed that spiromycins A–F exhibit potent antibacterial activities, with MIC values as low as 0.5 μM, whereas spoanthraquinones A (7) and B (8) display significant antioxidant activity in a DPPH· radical scavenging assay, with IC50 values of 27.23 ± 1.69 μM and 61.65 ± 1.37 μM, respectively. Overall, this work validates that reintroduction of endogenous BGCs represents a robust genome mining approach to activate silent metabolic pathways and accelerate the discovery of bioactive novel natural products.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1902–1912"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Assignment of Daucane Sesquiterpenoids by Low-Effort Total Synthesis and X-ray Crystallography 多环倍半萜类化合物的低合成和x射线晶体学结构分配。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-06-09 DOI: 10.1021/acs.jnatprod.6c00467

Adrian Dennis Podlesny, Josefine Brindöpke, Stephanie Schulz, David Mross, David Tymann, Ljuba Iovkova* and Martin Hiersemann*,

{"title":"Structural Assignment of Daucane Sesquiterpenoids by Low-Effort Total Synthesis and X-ray Crystallography","authors":"Adrian Dennis Podlesny, Josefine Brindöpke, Stephanie Schulz, David Mross, David Tymann, Ljuba Iovkova* and Martin Hiersemann*, ","doi":"10.1021/acs.jnatprod.6c00467","DOIUrl":"10.1021/acs.jnatprod.6c00467","url":null,"abstract":"Total synthesis is an indispensable tool for structural elucidation and thus a cornerstone of natural product research. Lasidiol esters are daucane sesquiterpenoids that have been isolated from various plant genera, particularly from the families Asteraceae and Apiaceae. The structure of lasidiol and one of its esters, lasidiol angelate, was assigned in 1981 by chemical correlation with carotol from Daucus carota. The structural assignment of three other natural lasidiol esters is based on spectral data comparison with lasidiol angelate. In this publication, we validate the previously reported structural characterization of three natural lasidiol esters through a combination of total synthesis and X-ray crystallography. The molecular structures of lasidiol and lasidiol angelate were determined by X-ray crystallography. During the process of structural validation, we were compelled to correct the previously proposed structure of a lasidiol ester derived from Ambrosia trifida. To enable a plant-independent supply of lasidiol and its esters, we devise a seven-step ex-chiral-pool total synthesis to (−)-lasidiol, thereby enabling straightforward access to a wide variety of lasidiol derivatives.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1963–1971"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the Expression Profiles of Borneol Dehydrogenase Genes and Its Catalytic Function in Camphor Biosynthesis in Lavender 薰衣草中冰片脱氢酶基因表达谱及其在樟脑生物合成中的催化作用分析。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-06-08 DOI: 10.1021/acs.jnatprod.6c00239

Yuling Zeng, Jing Nie, Haiyan Lan, Yichao Zhang, Yufang Dai, Xiaojie Cao, Liang Zhang and Jianjian Hu*,

{"title":"Analysis of the Expression Profiles of Borneol Dehydrogenase Genes and Its Catalytic Function in Camphor Biosynthesis in Lavender","authors":"Yuling Zeng, Jing Nie, Haiyan Lan, Yichao Zhang, Yufang Dai, Xiaojie Cao, Liang Zhang and Jianjian Hu*, ","doi":"10.1021/acs.jnatprod.6c00239","DOIUrl":"10.1021/acs.jnatprod.6c00239","url":null,"abstract":"Camphor is a key quality determinant of lavender oil. Bornyl dehydrogenase (BDH) catalyzes the final camphor biosynthesis step, yet its gene family and mechanism in lavender remain uncharacterized. We phylogenetically classified 154 short-chain dehydrogenase/reductase (SDR) genes into six clades and screened 17 BDH genes. Integrated transcriptomic and metabolomic analyses revealed that camphor biosynthesis primarily occurs via the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway, with coordinated expression of geranyl diphosphate synthase (GPPS), bornyl diphosphate synthases (BPPS), bornyl-diphosphate diphosphatase (BDD), and BDH genes. Most biosynthetic genes, especially BDH and BPPS, were significantly upregulated in L. × intermedia “Xinxun 4”, correlating with its highest camphor content. Subcellular localization showed BDH1 resides in the cytoplasm, suggesting plastid-cytoplasm collaboration. In vitro assays demonstrated that BDH1 and BDH2 both oxidize (+)- and (−)-borneol to camphor. Molecular dynamics simulations revealed that BDH1’s active site exhibits conformational flexibility, accommodating both enantiomers with similar binding affinities, which was confirmed by enzyme kinetics. Asn90 was identified as a key catalytic residue. These findings reveal BDH family expansion, coordinated gene expression driving camphor accumulation, and BDH1’s catalytic flexibility, offering key genetic resources and mechanistic insights for optimizing lavender camphor content via metabolic engineering and molecular breeding.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1785–1801"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148196519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chalcone-Terpene Hybrids from Fissistigma litseifolium (King) Merr as Potential Carbapenemase Inhibitors 小叶裂柄查尔酮-萜烯杂合体作为潜在碳青霉烯酶抑制剂的研究。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-30 DOI: 10.1021/acs.jnatprod.6c00338

Isabel Ramón Martínez, Cécile Apel, Saoussen Oueslati, Gaëtan Herbette, Jean-François Gallard, Emily Cioni, Azeana Zahari, Thierry Naas, Sandy Desrat and Fanny Roussi*,

{"title":"Chalcone-Terpene Hybrids from Fissistigma litseifolium (King) Merr as Potential Carbapenemase Inhibitors","authors":"Isabel Ramón Martínez, Cécile Apel, Saoussen Oueslati, Gaëtan Herbette, Jean-François Gallard, Emily Cioni, Azeana Zahari, Thierry Naas, Sandy Desrat and Fanny Roussi*, ","doi":"10.1021/acs.jnatprod.6c00338","DOIUrl":"10.1021/acs.jnatprod.6c00338","url":null,"abstract":"Resistance to carbapenems in Gram-negative bacteria is a major public health concern. This resistance is primarily mediated by carbapenemase enzymes, which hydrolyze and inactivate carbapenem antibiotics. These enzymes fall into two main categories: serine carbapenemases (e.g., OXA-48 and KPC-2) and zinc-dependent metallo-β-lactamases (e.g., NDM-1). Over recent years, their dissemination has dramatically increased worldwide, accompanied by the continuous emergence of novel variants. Notably, KPC-2 and OXA-48 are among the most prevalent carbapenemases. The current combinations of antibiotics and inhibitors available are insufficient to counteract the rapid spread of these enzyme variants. In this context, the bark extract of the Malasyan Fissistigma litseifolium (King) Merr, was selected following the screening of a large collection of plant extracts. This study describes and compares the metabolomic profile of this extract, for which no previous studies have been reported, with those of 13 other Fissistigma species. Phytochemical investigation resulted in the isolation of nine compounds, including three unreported natural products. Of these, fissisteifolin, a quinone, features a unique tetracyclic structure deriving from a chalcone and an uncommon monoterpene. Finally, fissistidin, an original chalcone-sesquiterpene dimer, exhibited 83% reduction in KPC-2 enzyme activity, establishing its potential as a candidate for developing novel carbapenemase inhibitors.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1883–1892"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148100696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytotoxic Synurins A–C: Chlorinated Naphthoquinone Pigments from the Freshwater Alga Synura sphagnicola 细胞毒性Synurins A-C：来自淡水藻藻的氯化萘醌色素。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-26 DOI: 10.1021/acs.jnatprod.6c00417

Magda Škaloudová, Jan Blahut, Jan Hájek, Alan Kádek, Peter Mojzeš, Jana Pilátová, Dominika Tučková, Petra Divoká, Antonín Střížek, Martin Lukeš, Eva Kotabová, Petra Bittnerová, Kumar Saurav, Pavel Hrouzek and Petra Urajová*,

{"title":"Cytotoxic Synurins A–C: Chlorinated Naphthoquinone Pigments from the Freshwater Alga Synura sphagnicola","authors":"Magda Škaloudová, Jan Blahut, Jan Hájek, Alan Kádek, Peter Mojzeš, Jana Pilátová, Dominika Tučková, Petra Divoká, Antonín Střížek, Martin Lukeš, Eva Kotabová, Petra Bittnerová, Kumar Saurav, Pavel Hrouzek and Petra Urajová*, ","doi":"10.1021/acs.jnatprod.6c00417","DOIUrl":"10.1021/acs.jnatprod.6c00417","url":null,"abstract":"Synura sphagnicola, a peat bog chrysophyte, forms conspicuous red cytoplasmic droplets. These droplets previously showed an unidentified pigment, prompting a detailed chemical investigation. Large-scale cultivation and chromatographic purification yielded three structurally related orange-to-red compounds, previously undescribed chlorinated naphthoquinone pigments─synurin A (1), synurin B (2), and synurin C (3)─whose identities were established using HPLC-HRMS, FT-ICR MS, NMR spectroscopy, FTIR, fluorescence spectroscopy, and Raman microscopy. The compounds were identified as 2,3-dichloro-5,8-dihydroxy-6-propylnaphthalene-1,4-dione (1) and its two O-methylated regioisomers (2, 3). Raman spectra revealed strong environment-dependent vibrational differences, particularly for 1 and 2. In vitro assays demonstrated potent but nonselective cytotoxicity toward cancerous and nontransformed cell lines (IC50 in the low micromolar range), while only 1 exhibited ferric-reducing antioxidant activity.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1932–1941"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.6c00417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148024896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of Global Regulator ShCrp Leads to the Discovery of Polycyclic Tetramate Macrolactams in Streptomyces hebeiensis HDN10-293 全球调控因子ShCrp的过表达导致河北链霉菌HDN10-293中发现了多环四聚内酰胺。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-18 DOI: 10.1021/acs.jnatprod.6c00138

Ruojin Liu, Chuanteng Ma, Yueling Teng, Wenxue Wang, Xiaomin Zhang, Dehai Li, Tianjiao Zhu* and Qian Che*,

引用次数: 0