Journal of Natural Products 最新文献_第10页

Systematic Assessment of the Impact of Targeted Selection Methods and Environment-Mimicking Culture Conditions on Fungal Natural Product Libraries 目标选择方法和模拟环境培养条件对真菌天然产物文库影响的系统评价。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-22 DOI: 10.1021/acs.jnatprod.6c00295

Monica Ness, Karen L. Wendt, Thilini Peramuna, Demetrius I. Tillery, Jillian E. Murray, Robert H. Cichewicz* and Laura-Isobel McCall*,

{"title":"Systematic Assessment of the Impact of Targeted Selection Methods and Environment-Mimicking Culture Conditions on Fungal Natural Product Libraries","authors":"Monica Ness, Karen L. Wendt, Thilini Peramuna, Demetrius I. Tillery, Jillian E. Murray, Robert H. Cichewicz* and Laura-Isobel McCall*, ","doi":"10.1021/acs.jnatprod.6c00295","DOIUrl":"10.1021/acs.jnatprod.6c00295","url":null,"abstract":"Natural products are a rich source of bioactive molecules and undiscovered chemical scaffolds with significant potential for drug discovery. Among producers of natural products, fungi are particularly promising, offering diverse metabolites and undiscovered structural motifs. Large, well-curated collections of crude extracts, or “libraries”, are central to fungal natural product discovery, serving as starting material for bioassay-guided isolation of new compounds. However, the systematic influence of fungal selection strategies, culturing methods, and environmental factors on chemical diversity remains underexplored. In this study, we analyzed several large fungal libraries to assess how geographic origin and phylogenetic classification shape fungal chemical profiles. We also evaluated whether culturing conditions that more closely mimic natural environments can enhance metabolite diversity. Our findings offer practical guidelines for optimizing fungal natural product library design, improving drug development efficiency and accessing novel chemotypes for future drug discovery.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1821–1833"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.6c00295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147985519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic C-Acylation Enables Diversification of Tetramate Antibiotic Scaffolds 酶促c -酰化使四聚体抗生素支架多样化。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-14 DOI: 10.1021/acs.jnatprod.6c00215

Anfu Wei, Chengneng Mi, Meihan Liu, Ruolan Sun, Yong Nian, Beibei Li*, Ge Liao* and Xiaoyu Tang*,

{"title":"Enzymatic C-Acylation Enables Diversification of Tetramate Antibiotic Scaffolds","authors":"Anfu Wei, Chengneng Mi, Meihan Liu, Ruolan Sun, Yong Nian, Beibei Li*, Ge Liao* and Xiaoyu Tang*, ","doi":"10.1021/acs.jnatprod.6c00215","DOIUrl":"10.1021/acs.jnatprod.6c00215","url":null,"abstract":"Tetramate (pyrrolidine-2,4-dione) scaffolds are widely distributed among bacterial natural products, including metabolites produced by human-associated microorganisms, where they contribute to microbial competition and ecological fitness. Enzymatic tailoring reactions play important roles in diversifying these bioactive scaffolds; however, enzymatic C-acylation has thus far been largely restricted to Friedel–Crafts-type reactions on aromatic substrates. Here, we report the identification and characterization of a family of bacterial acyltransferases from distinct bacterial species, including Streptococcus macacae (SmaATase), Lactococcus hircilactis (LhATase), Priestia megaterium (PmATase), and Bacillus pseudomycoides (BpATase). These enzymes exhibit broad tolerance toward tetramate acceptors and phenyl acyl ester donors, enabling enzymatic diversification beyond classical aromatic frameworks. Selected acylated products retained or modestly improved antibacterial activity against methicillin-resistant Staphylococcus aureus, highlighting the potential of this enzymatic platform for expanding the chemical space of tetramate metabolites. Together, this work establishes an enzymatic strategy for diversification of tetramate natural product scaffolds and expands the scope of enzymatic C–C bond formation in microbial natural product chemistry.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1756–1763"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147924767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spiropatuols A–H: Polycyclic Polyprenylated Acylphloroglucinols from Hypericum patulum with Activity against Concanavalin A–Induced Autoimmune Hepatitis 螺旋体醇A-H：从金丝桃中提取的多环聚丙烯化酰基间苯三酚，具有抗刀豆蛋白a诱导的自身免疫性肝炎的活性。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-20 DOI: 10.1021/acs.jnatprod.6c00204

Zhengyi Shi, Xiangli Zhao, Jiangchun Wei, Xiaosheng Tan, Zhangrong Hou, Yi Guo, Gang Chen*, Changxing Qi*, Jianping Wang* and Yonghui Zhang*,

{"title":"Spiropatuols A–H: Polycyclic Polyprenylated Acylphloroglucinols from Hypericum patulum with Activity against Concanavalin A–Induced Autoimmune Hepatitis","authors":"Zhengyi Shi, Xiangli Zhao, Jiangchun Wei, Xiaosheng Tan, Zhangrong Hou, Yi Guo, Gang Chen*, Changxing Qi*, Jianping Wang* and Yonghui Zhang*, ","doi":"10.1021/acs.jnatprod.6c00204","DOIUrl":"10.1021/acs.jnatprod.6c00204","url":null,"abstract":"Eight new spiropolycyclic polyprenylated acylphloroglucinols, named spiropatuols A–H (1–8), were isolated from the traditional Chinese medicinal herb Hypericum patulum. The structures of compounds 1–8 were characterized using spectroscopic analysis, calculations of nuclear magnetic resonance chemical shifts with DP4+ probability analysis, and electronic circular dichroism calculations. Compounds 2–4 exhibited immunosuppressive activities in an in vitro model of concanavalin A (Con A)–induced murine splenocyte proliferation, with half-maximal inhibitory concentrations of 50, 70, and 200 nM, respectively. Furthermore, in a Con A–challenged autoimmune hepatitis mouse model, compound 2 inhibited Con A–induced activation of mice splenic lymphocytes and alleviated Con A–induced acute liver injury. These results indicate that the metabolites isolated in this study can inform the design of novel immunosuppressors for treating autoimmune hepatitis.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1764–1774"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147968714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Lipidomic Analysis of Streptomyces by NPLC-HRMS Reveals a Previously Unknown Phospholipid Class 利用液相色谱- hrms对链霉菌进行全面的脂质组学分析，揭示了一个以前未知的磷脂类。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-06-03 DOI: 10.1021/acs.jnatprod.5c01603

Sonia Abreu, Clara Lejeune, Colin Grunberger, Enora Jaffrezic, Marie-Joelle Virolle and Pierre Chaminade*,

引用次数: 0

Evolution-Oriented Modular Excision of Polyketide Synthases at a Splice Site of Enoylreductase Domain Yielded an Anti-Inflammatory Carboxyl Polyether 以进化为导向的在烯基还原酶结构域剪接位点上的聚酮合成酶的模块化切除产生了抗炎羧基聚醚。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-29 DOI: 10.1021/acs.jnatprod.6c00405

Chang Xu, Li-Li Hong, Jing Xu, Haixia Zang, Wenrui Peng, Hou-Wen Lin* and Yongjun Zhou*,

{"title":"Evolution-Oriented Modular Excision of Polyketide Synthases at a Splice Site of Enoylreductase Domain Yielded an Anti-Inflammatory Carboxyl Polyether","authors":"Chang Xu, Li-Li Hong, Jing Xu, Haixia Zang, Wenrui Peng, Hou-Wen Lin* and Yongjun Zhou*, ","doi":"10.1021/acs.jnatprod.6c00405","DOIUrl":"10.1021/acs.jnatprod.6c00405","url":null,"abstract":"Polyketide natural products represent a major source of medicinal compounds. The majority of them are biosynthesized by the modular polyketide synthase (PKS) assembly line in Streptomyces spp. Due to the high sequence similarity among the coding regions of PKSs, recombination-mediated gene truncation is prevalent in PKSs. These recombination sites can be the guidelines of reprogramming PKSs for designed biosynthesis. Herein, we have discovered that a PKS module excision naturally occurred between two adjacent enoylreductase (ER) domains in the PKS assembly line of carboxyl polyether ionomycin (INO). The recombination led to significant accumulation of a two-carbon truncated product of INO, neoionomycin A, which exhibited significant anti-inflammatory activities in both zebrafish neuroinflammation and mouse colitis models. Moreover, the natural fusion site of the ER domain was successfully applied to generate the second PKS module excision, yielding another truncated product of INO. The study should represent the first instance of generating the functional hybrid PKS module via a splice junction within the ER domain, thereby adding a new reference site for the rational PKS reconstruction.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1924–1931"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148051232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structurally Diverse Secondary Metabolites from the Stem of Tinospora cordifolia and Their Anti-Inflammatory Potential on RAW264.7 Macrophages Tinospora cordifolia茎次生代谢物的结构多样性及其对RAW264.7巨噬细胞的抗炎作用

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-04-21 DOI: 10.1021/acs.jnatprod.6c00126

Dixhya Rani, Diksha Saroch, Anil Bhushan, Manish Kumar Sharma, Bashir Ahmad Lone, Urvashi Dhiman, Gurleen Kour, Zabeer Ahmed* and Prasoon Gupta*,

{"title":"Structurally Diverse Secondary Metabolites from the Stem of Tinospora cordifolia and Their Anti-Inflammatory Potential on RAW264.7 Macrophages","authors":"Dixhya Rani, Diksha Saroch, Anil Bhushan, Manish Kumar Sharma, Bashir Ahmad Lone, Urvashi Dhiman, Gurleen Kour, Zabeer Ahmed* and Prasoon Gupta*, ","doi":"10.1021/acs.jnatprod.6c00126","DOIUrl":"10.1021/acs.jnatprod.6c00126","url":null,"abstract":"Six new secondary metabolites were isolated from the stem extract of Tinospora cordifolia, including two polyoxygenated sterols, cordisterone A (1) and cordisterone B (3), two terpenes, cordipene A (2) and cordipene B (4), a C-glucoside, cordiside A (5), rare 2-(4-amino-2-hydroxybutyl) benzoic acid, cordizoic acid (6), and 18 (7–24) known compounds. Compounds 7 and 8 were isolated for the first time from this plant. Their structures, including absolute configurations, were determined by detailed examination of 1D/2D NMR, HRESIMS spectroscopic data, optical rotation, and electronic circular dichroism (ECD) calculations. Extracts, fractions, and pure compounds were evaluated for their nitric oxide inhibitory effects in lipopolysaccharide-activated RAW264.7 cells. Cordiside A (5) significantly inhibited nitric oxide (NO) production in murine macrophage cells with an IC50 value of 3.79 ± 0.68 μM and exhibited low cytotoxicity (IC50 > 50 μM), whereas 2 and 6 showed moderate activity. Cordiside A (5) also inhibited interleukin-6 (IL-6) and TNF-α significantly at the concentration of 12.5 μM with the IC50 values of 5.12 ± 0.56 and 8.61 ± 0.69 μM, respectively. This study disclosed the discovery and structure characterization of six new compounds and the significant therapeutic potential of the new C-glycoside (5) in combating inflammation-related diseases.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1724–1737"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147757513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteotoxic Stress Bioreporter Enables Mechanism-Informed Antibiotic Discovery 蛋白毒性应激生物报告使机制知情的抗生素发现。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-15 DOI: 10.1021/acs.jnatprod.6c00112

Julian Schubert, Christian Geibel, Anne Berscheid, Katharina W. Wex, Giovanni Andrea Vitale, Chambers C. Hughes, Daniel Petras* and Heike Brötz-Oesterhelt*,

{"title":"Proteotoxic Stress Bioreporter Enables Mechanism-Informed Antibiotic Discovery","authors":"Julian Schubert, Christian Geibel, Anne Berscheid, Katharina W. Wex, Giovanni Andrea Vitale, Chambers C. Hughes, Daniel Petras* and Heike Brötz-Oesterhelt*, ","doi":"10.1021/acs.jnatprod.6c00112","DOIUrl":"10.1021/acs.jnatprod.6c00112","url":null,"abstract":"Antibiotic resistance poses a major global health challenge, which is intensified by the decline in antibiotic discovery efforts. This highlights the urgent need for innovative antibacterial agents. Natural products remain a key source of antibiotics, yet early mechanistic insights are often lacking. Classical whole-cell screening identifies growth inhibition but provides no mechanistic information. Bioreporter strains combine bioactivity detection with preliminary mechanistic profiling; however, they remain unavailable for several promising antibiotic target areas. We developed a sensitive bioreporter that signals the induction of the heat-shock Clp-ATPase gene clpE in Bacillus subtilis. ClpE serves as a specific biomarker for proteotoxic stress, resulting from the accumulation of damaged or misfolded proteins. The self-sustained luminescence of the PclpE-lux bioreporter enables monitoring of proteotoxic stress in both solid and liquid assay formats and supports high-throughput screening. Consolidated through extensive validation with reference antibiotics, the bioreporter also identified antibacterial agents that were not previously associated with proteotoxic stress. Coupling the bioreporter with a microfractionation-based metabolomics workflow accelerated the identification of hydrophilic antibacterial metabolites from complex natural product extracts, thereby uncovering an extensive group of streptothricin derivatives, including putatively novel analogues. This study demonstrates that the PclpE-lux bioreporter is a versatile tool for mechanism-informed antibiotic discovery, enhancing the detection sensitivity and dereplication efficiency.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1698–1710"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acs.jnatprod.6c00112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147947203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Chain Length on the Antimicrobial Properties of Dialkylresorcinols 链长对二烷基间苯二酚抗菌性能的影响。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-06-01 DOI: 10.1021/acs.jnatprod.6c00306

Jiao Yang, Sandra Tienda, Francisco M. Cazorla and Benjamin R. Clark*,

引用次数: 0

Total Synthesis and Structural Revision of (−)-Garvensintriol （-）-加文辛三醇的全合成及结构修正。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-06-03 DOI: 10.1021/acs.jnatprod.6c00456

Yifei Zhang, Chuanfang Zhao, Bitao Wei, Linjin Li, Pengpeng Nie, Jun Liu* and Yuguo Du,

引用次数: 0

Enantiomeric Prenylated Neolignans from the Roots of Illicium verum with Analgesic Activity 具有镇痛作用的茴香根对映体戊烯化新木脂素。

IF 3.7 2区生物学

Journal of Natural Products Pub Date : 2026-06-26 Epub Date: 2026-05-28 DOI: 10.1021/acs.jnatprod.6c00208

Li Zou, Zhi-Hui Luo, Ying Chen, Shu-Li Duan, Qi-Hang Luo, Yu-Xiang Gao, Liang-Yan Xiao, Hong-Xia Fan, Zheng-Qun Zhou* and Hao Gao*,

{"title":"Enantiomeric Prenylated Neolignans from the Roots of Illicium verum with Analgesic Activity","authors":"Li Zou, Zhi-Hui Luo, Ying Chen, Shu-Li Duan, Qi-Hang Luo, Yu-Xiang Gao, Liang-Yan Xiao, Hong-Xia Fan, Zheng-Qun Zhou* and Hao Gao*, ","doi":"10.1021/acs.jnatprod.6c00208","DOIUrl":"10.1021/acs.jnatprod.6c00208","url":null,"abstract":"Two pairs of unprecedented enantiomeric prenylated neolignans (1a/1b and 2a/2b) with an unparalleled 9.2′,8.O.3′-linked skeleton were isolated from the roots of Illicium verum, along with three biogenetically related metabolites (3a/3b and 4). The structures of these compounds were elucidated using spectroscopic techniques, X-ray crystallography analysis, and quantum chemical calculations. Both 2,4,5-trioxygenated phenylpropanoids (represented by 4) and their analogues with dearomatization of the benzene ring (represented by 3) are uncommon. Compounds 1a/1b and 2a/2b are likely produced by their dimerization. Inspired by the traditional effects of I. verum’s fruits, the analgesic activities of compounds 1–4 were evaluated using a glacial acetic acid-induced zebrafish pain model. The results indicated that all compounds showed different levels of analgesic activity. A major and active prenylated 2,4,5-trioxygenated phenylpropanoid with the dearomatization of the benzene ring (3) exhibited dose-dependent analgesic activity in a formalin-induced mouse paw licking assay. The analgesic potency of compound 3 is comparable to that of the positive drug diclofenac sodium (DCS) under the same dose (10 mg/kg). The analgesic potencies of compounds 3, 3a, and 3b were also comparable under a 2 mg/kg dose. The analgesic activities of prenylated phenylpropanoids with dearomatization of the benzene ring are first reported herein.","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"89 6","pages":"1747–1755"},"PeriodicalIF":3.7,"publicationDate":"2026-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148051310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0