laeA Gene Introduction Strategy Enabling the Construction of a Prolific Fungal Secondary Metabolite Library.

IF 3.3 2区生物学 Q2 CHEMISTRY, MEDICINAL

Journal of Natural Products Pub Date : 2025-03-28 Epub Date: 2025-02-20 DOI:10.1021/acs.jnatprod.4c01317

Aoi Kimishima, Sota Honma, Satoshi Kato, Masako Honsho, Hiroki Kojima, Toshiyuki Tokiwa, Akihiro Sugawara, Kenichi Nonaka, Yasuko Araki, Tadashi Takahashi, Kotaro Ito, Yukihiro Asami

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引用次数: 0

Abstract

LaeA is a putative nuclear methyltransferase protein that epigenetically influences secondary metabolite production in fungi. LaeA has drawn attention as one of the promising approaches to activate fungal chemical production, and the laeA gene has been introduced into some fungal strains with the aim of producing secondary metabolic changes mainly based on the evaluation of mycotoxicity. However, these studies were applied for limited fungal species, and its utility and versatility for broad fungal species remained unclear. In this study, 47 strains were selected composed of three different genera, Pochonia spp., Gamszarea kalimantanensis, and Lecanicillium spp., which have never been modified with the laeA gene. We obtained a total of 125 mutants with laeA genes for our fungal strain library. The chemical productivity and the biological activity of the library were analyzed, and two natural products, radicicol (1) and sch210972 (2), were isolated in more than 10-fold the yield of the parent strains.

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laeA基因导入策略构建高产真菌次生代谢物文库。

LaeA是一种假定的核甲基转移酶蛋白，在表观遗传上影响真菌次生代谢物的产生。LaeA作为一种很有前途的激活真菌化学产物的方法而受到关注，LaeA基因已被引入到一些真菌菌株中，主要是基于真菌毒性的评价，目的是产生次生代谢变化。然而，这些研究仅应用于有限的真菌种类，其对广泛真菌种类的实用性和通用性尚不清楚。本研究选取了Pochonia spp.、Gamszarea kalimantantensis .和Lecanicillium spp. 3个不同属的47株未被laeA基因修饰的菌株。我们共获得125个具有laeA基因的突变体，用于真菌菌株库。对文库的化学生产力和生物活性进行了分析，分离得到两种天然产物radicicol(1)和sch210972(2)，其产量是亲本菌株的10倍以上。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.