Saudi Dental Journal最新文献

{"title":"Favorable Strategies for oral biology learning from dental students’ perception","authors":"Amani M. Harrandah","doi":"10.1016/j.sdentj.2024.12.002","DOIUrl":"10.1016/j.sdentj.2024.12.002","url":null,"abstract":"<div><div>Oral biology is an important tool in clinical dental training. Therefore, a good understanding of oral biology is crucial for improving the outcomes of dental education. This study aims to assess dental students’ perceptions of the relevance of oral biology in dentistry and investigate their attitudes toward learning and study approaches. We also investigated the acceptance of each teaching method and study resources.</div></div><div><h3>Materials and methods</h3><div>A Likert scale-based online questionnaire was administered to undergraduate dental students, dental interns and to a group of postgraduate dental students at the College of Dentistry, Umm AlQura university in the end of the academic year.</div><div>Results and conclusion:</div><div>The results indicated that most dental students consider oral biology relevant to dental education and clinical practice. Academic lectures appear to be the most favorable learning method for dental students, followed by practical sessions. Regarding preferred study resources, handouts appeared to be the most preferred, followed by online resources. Furthermore, it appears that students’ preferences for teaching methods and study resources often vary according to their academic level; overall, it appears that students depend more on passive learning than active learning.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1651-1654"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing predictive analytics in mandibular third molar extraction using artificial intelligence: A CBCT-Based study","authors":"Faezeh Khorshidi ,&nbsp;Rasool Esmaeilyfard ,&nbsp;Maryam Paknahad","doi":"10.1016/j.sdentj.2024.11.007","DOIUrl":"10.1016/j.sdentj.2024.11.007","url":null,"abstract":"<div><h3>Objective</h3><div>Forecasting the complexity of extracting mandibular third molars is crucial for selecting appropriate surgical methods and minimizing postoperative complications. This study aims to develop an AI-driven predictive model using CBCT reports, focusing specifically on predicting the difficulty of mandibular third molar extraction.</div></div><div><h3>Methods</h3><div>We conducted a retrospective study involving 738 CBCT reports of mandibular third molars. The data was divided into a training set consisting of 556 reports and a validation set containing 182 reports. The study involved two main steps: pre-processing and processing of the textual data. During pre-processing, the reports were cleaned and standardized. In the processing phase, a rule-based NLP algorithm was employed to identify relevant features such as angulation, number of roots, root curvature, and root-nerve canal relationship. These features were utilized for the training of a deep learning neural network to classify the extraction difficulty into four categories: easy, slightly difficult, moderately difficult, and very difficult.</div></div><div><h3>Results</h3><div>The classification model achieved an accuracy of 95% in both the training and validation sets. Precision, recall, and F1-score metrics were calculated, yielding promising results with precision and recall values of 0.97 and 0.95 for the training set, and 0.97 and 0.89 for the validation set, respectively.</div></div><div><h3>Conclusion</h3><div>The study demonstrated the high reliability of AI-based models to forecast the complexity of the mandibular third molar extractions from CBCT reports. The results indicate that AI-driven models can accurately predict extraction difficulty, thereby aiding clinicians in making informed decisions and potentially improving patient outcomes.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1582-1587"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malocclusion traits as risk indicators for depression in young adults: A population-based cross-sectional study","authors":"Ahmed A. Alsulaiman","doi":"10.1016/j.sdentj.2024.09.018","DOIUrl":"10.1016/j.sdentj.2024.09.018","url":null,"abstract":"<div><h3>Background</h3><div>Although malocclusion has historically drawn attention for its effects on craniofacial development, oral function, and aesthetics, recent studies have started to investigate the possible association between malocclusion and other aspects of overall health, such as mental health.</div></div><div><h3>Objectives</h3><div>This study aimed to analyze various dental malocclusion characteristics and their potential association with depressive symptoms among young adults. The National Health and Nutrition Examination Survey was used for data procurement.</div></div><div><h3>Methods</h3><div>This cross-sectional study was conducted using a nationally representative population sample, including data from 3,806 participants surveyed between 1988 and 1994 under the National Health and Nutrition Examination Survey III. The inclusion criteria were completion of the clinical assessment for malocclusion traits, being dentate, reporting no history of previous or current orthodontic treatment, reporting no dental pain, and responding to a questionnaire about psychiatric status. Statistical evaluations involved logistic regression modeling and chi-squared tests, considering the complex sampling design nature of the survey.</div></div><div><h3>Results</h3><div>Crude and adjusted logistic regression models indicated no significant association between malocclusion traits and major depressive episodes (MDEs). Conversely, the same models revealed a positive association between malocclusion traits (specifically, upper crowding and two or more cumulative malocclusion traits) and dysthymia in both crude and adjusted analyses.</div></div><div><h3>Conclusion</h3><div>Specific malocclusion traits were associated with dysthymia, whereas no such associations existed with MDEs. These findings suggest that the association between malocclusion and depression is complex and multifaceted.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1527-1532"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cross-sectional study on dentists’ learning preferences for learning about light-curing units and resin-based composites","authors":"Afnan O. Al-Zain ,&nbsp;Khlood Baghlaf ,&nbsp;Omar Abdulwassi ,&nbsp;Reem Almukairin ,&nbsp;Maram Alanazi ,&nbsp;Elaf Alshomrani ,&nbsp;Sultan Alftaikhah ,&nbsp;Richard B. Price","doi":"10.1016/j.sdentj.2024.10.002","DOIUrl":"10.1016/j.sdentj.2024.10.002","url":null,"abstract":"<div><h3>Introduction</h3><div>Despite researchers emphasizing the importance of understanding the type, how they should be used, and their effect on restoration longevity, many dentists lack critical knowledge about their light curing units (LCUs).</div></div><div><h3>Aim</h3><div>To identify the parameters dentists use when choosing an LCU or resin-based composite (RBC) and to determine the most effective educational method for dentists to learn about LCUs.</div></div><div><h3>Methods</h3><div>A cross-sectional study used a validated electronic questionnaire targeting dentists, interns, and students in private and government sectors. Demographic data, current LCU practices, parameters for selecting LCUs and RBCs, and the preferred educational methods for learning about LCUs were collected. The three main parameters used to determine participants’ knowledge of LCU selection were brand reputation, irradiance, and LCU wavelength compatibility with the RBC photoinitiator spectrum. Results were analyzed using chi-square test, Fisher’s exact test, and independent sample <em>t</em>-test.</div></div><div><h3>Results</h3><div>A total of 420 participants completed the survey. Only 11 % considered the wavelength as the 1st parameter when selecting an LCU. Participants with graduate degrees were significantly more knowledgeable about the LCU selection parameters than those with bachelor’s degrees (p = 0.009). Dentists who frequently used LCUs knew significantly more about the RBC selection parameters (p = 0.037). Hands-on workshops, in-person lectures, and online lectures were preferred over social media. YouTube is one of the most popular social media platforms. All participants preferred YouTube as the top social media educational resource, with specialists and consultants showing a significantly stronger preference than general dentists, interns, and students (p = 0.036).</div></div><div><h3>Conclusion</h3><div>Few participants thought that the need for the wavelengths emitted by the LCU to be compatible with the absorption spectrum of the photoinitiator used in the RBC was a key parameter when selecting an LCU or RBC. Respondents favored hands-on workshops and in-person lectures over social media to learn about LCUs. Educators should prioritize these methods to enhance understanding the choice and use of LCUs and RBCs within the dental community.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1673-1680"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of fixed and removable dental prostheses on the periodontal health of abutment teeth: A Retro-Prospective Comparative Study","authors":"Arwa U. Alsaggaf ,&nbsp;Ahlam Almuwallad ,&nbsp;Lina Mirza ,&nbsp;Layan Alhemayed ,&nbsp;Halema Zamzami ,&nbsp;Ehab Azab ,&nbsp;Hesham Alhazmi ,&nbsp;Amin Marghalani","doi":"10.1016/j.sdentj.2024.11.010","DOIUrl":"10.1016/j.sdentj.2024.11.010","url":null,"abstract":"<div><h3>Introduction</h3><div>Periodontal disease is the most prevalent oral condition worldwide, resulting from a prolonged inflammatory reaction triggered by the buildup of dental plaque. Many studies have concluded that there is a strong association between periodontal health and tooth restorations, as the periodontium must be healthy for the restoration to last.</div></div><div><h3>Aim</h3><div>to evaluate the impact of fixed and removable dental prostheses on the periodontal health of abutment teeth.</div></div><div><h3>Methodology</h3><div>This was a <em>retro</em>-prospective cohort study of 112 abutment teeth collected from 34 patients who received a total of 47 fixed (81 abutments) and 14 removable (31 abutments) dental prostheses treated by senior dental students under supervision during the past two years. Patients were recalled for clinical and radiographic examinations to assess periodontal parameters of abutments, including pocket depth (PD), clinical attachment loss (CAL), presence of plaque, and bleeding on probing (BOP), and to evaluate fixed and removable prostheses.</div></div><div><h3>Results</h3><div>Most of the prostheses were fixed (72.32 %), and most of them were bridges (60.71 %) located in the anterior region of the mouth (58.56 %). The study findings showed a significant increase (mean = 0.46 mm) in clinical attachment loss after prosthesis placement (p &lt; 0.01), while no significant difference was observed in pocket depth (p = 0.062). Furthermore, there was a significant increase in plaque at the follow-up visit compared to the baseline visit (p &lt; 0.01) for only the fixed dental prostheses. There was no significant difference between fixed and removable prostheses regarding their impact on the periodontal health of abutments.</div></div><div><h3>Conclusion</h3><div>The study found that abutment teeth had increased clinical attachment loss after receiving dental prostheses, highlighting the need for regular maintenance and patient education to maintain periodontal health.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1588-1592"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of final irrigation with titanium dioxide and silver nanoparticles on the fracture resistance of endodontically treated roots","authors":"Mohamed I. Ebrahim ,&nbsp;Fahd M. Hadhoud ,&nbsp;Ali A. Alqarni ,&nbsp;Shaimaa M. Al Harthi ,&nbsp;Hassan T. Shawli","doi":"10.1016/j.sdentj.2024.10.004","DOIUrl":"10.1016/j.sdentj.2024.10.004","url":null,"abstract":"<div><h3>Background</h3><div>Titanium dioxide (TiO2NP) and silver nanoparticles (AgNPs) have been recognized for their ability to enhance the structural integrity of dental materials. This study aimed to compare the influence of these nanoparticles on the structural resilience of treated root canals.</div></div><div><h3>Materials and Methods</h3><div>The crowns of 75 undamaged central incisors were sectioned below the cemento-enamel junction to achieve a consistent root length of 16 mm. The root canals were prepared using K3 files up to size 40 with a 6 % taper. The specimens were then divided into five groups (n = 15), each subjected to a final irrigation protocol with one of the following solutions: normal saline, 2 % chlorhexidine (CHX), 17 % EDTA combined with 2.5 % sodium hypochlorite (NaOCl), 17 % EDTA with 0.1 % silver nanoparticles (AgNPs), and 17 % EDTA with 0.1 % titanium dioxide nanoparticles (TNPs). After obturation with gutta-percha and sealer, fracture resistance (FR) was evaluated. The data were analyzed using one-way ANOVA and Tamhane’s post hoc test (p &lt; 0.050).</div></div><div><h3>Results</h3><div>The lowest fracture resistance (FR) was observed in the group treated with ethylenediaminetetraacetic acid (EDTA) combined with sodium hypochlorite (NaOCl), followed by the groups irrigated with normal saline and chlorhexidine (CHX), with no statistically significant difference between the latter two groups (p &gt; 0.05). There was also no significant difference in FR between the CHX and saline groups (p &gt; 0.05). In contrast, irrigation with silver nanoparticles (AgNPs) and titanium dioxide nanoparticles (TNPs) resulted in significantly higher fracture resistance compared to the other solutions tested (p &lt; 0.050).</div></div><div><h3>Conclusion</h3><div>The fracture resistance of endodontically treated roots was significantly improved when the final irrigation sequence included AgNPs and TNPs nanoparticles. In contrast, sodium hypochlorite (NaOCl) yielded the lowest fracture resistance value.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1549-1552"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The prevalence of mandibular lingual concavity among the Saudi population of Eastern Province: A CBCT evaluation","authors":"Bader Alzaben ,&nbsp;Khalid Almas ,&nbsp;Faisal E. Aljofi ,&nbsp;Abdulmajeed A. Aljabr ,&nbsp;Al Hanoof Alarfaj ,&nbsp;Leena I. Bin–Jardan ,&nbsp;Subraya Bhat Giliyar ,&nbsp;Eman Ahmed Aljoghaiman","doi":"10.1016/j.sdentj.2024.11.015","DOIUrl":"10.1016/j.sdentj.2024.11.015","url":null,"abstract":"<div><h3>Background</h3><div>Anatomic variations can make placing implants in the mandible more complex. Exploring the variation in the presence of ridges and lingual concavity among different races and ethnicities is of therapeutic significance. The current study evaluates the prevalence and size of mandibular lingual concavity, which can cause surgical complications while inserting dental implants in the mandibular first molar area.</div></div><div><h3>Methods</h3><div>Two hundred and fifty-six cross-section images of the edentulous first molar region were examined. Within this geographical area, the structure of the lower jaw was categorized into three distinct types: C-configuration (convex), P-configuration (parallel), and U-configuration (with an undercut). The study was conducted at IAU College of Dentistry, Dammam. Data was gathered on the depth of the lingual concavity, the angle of the concavity, and other relevant factors. The data were analyzed with SPSS 20. A p-value ≤ 0.05 was considered significant.</div></div><div><h3>Results</h3><div>Of 256 subjects included in the study, 144 were males (M), and 112 were females (F). Various variables measured complimenting the lingual concavity showed no difference between the genders, except for the VCB “The vertical distance from the alveolar crest to line A” (p-value = 0.005). The division of ridges morphology as a whole was as follows: Convex (C), 70 (27.34 %), Parallel (P) 51 (19.92 %), and Undercut (U) type, 135 (52.73 %). The angle of concavity was 69.28 ± 14.41 % (M) and 67.09 ± 13.04 %. (F). Angle depth was 2.40 ± 1.72 % (M) and 2.36 ± 1.46 % (F); together, 2.38 ± 1.6 %.</div></div><div><h3>Conclusion</h3><div>It was concluded that U-type ridges were predominant (52.73%) with more chances of occurrence of lingual concavity. There were no differences between the genders except in one parameter. (VCB) Further studies are required to explore lingual concavity in more detail.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1618-1622"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral hygiene indices and chronic kidney disease in children: Systematic review and meta-analysis of case-control studies","authors":"Narjes Amrollahi ,&nbsp;Mohammad javad Tarrahi ,&nbsp;Zahra Abbasi","doi":"10.1016/j.sdentj.2024.11.009","DOIUrl":"10.1016/j.sdentj.2024.11.009","url":null,"abstract":"<div><h3>Background</h3><div>Chronic kidney disease (CKD) is a disorder that causes numerous problems for children and affects many organs, as oral hard and soft tissues. The purpose of this study was to summarize the relationship between oral and dental health status and CKD in children.</div></div><div><h3>Materials and Methods</h3><div>A systematic search of texts from 2000 to 2023 was conducted to gather all case control studies published in the English language related to the subject of this study in PubMed, Web of Science, Scopus and Cochrane electronic databases. The title, abstract and full text of the articles were examined according to inclusion criteria until relevant studies were selected. This process was carried out independently by two researchers. The quality of the selected studies was assessed by the National Institutes of Health (NIH) checklist.</div></div><div><h3>Results</h3><div>A total of 768 articles were identified in electronic databases during the search process. Articles inconsistent with inclusion criteria and duplicate articles were removed and 8 studies were selected for <em>meta</em>-analysis. The results found no statistically significant difference in mean of decayed, missing and filled teeth in permanent (DMFT index) and primary teeth (dmft index) in CKD and healthy children with a mean difference of −0.433 (95 % CI: −1.689 to 0.823; p-value = 0.500) and-0.095 (95 % CI: −2.240 to 2.051; p-value = 0.931) respectively. However, CKD had a significant effect on the developmental defects of enamel (DDE) index in children with an effect size of 4.916 (95 % CI: 1.752 to 13.799; p-value = 0.002).</div></div><div><h3>Conclusion</h3><div>The presence of CKD can increase the incidence of DDE by 4.9 times, but it has no significant effect on the prevalence of dental caries in primary and permanent teeth.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1495-1502"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adapting to emergent paradigm shifts: A calibration study among graduating dental students using the new classification of periodontal diseases","authors":"Mohamed Roshdy Abdelrasoul ,&nbsp;Dina Abdel Hamid Derbala ,&nbsp;Ahmed Fouad Hassan","doi":"10.1016/j.sdentj.2024.10.006","DOIUrl":"10.1016/j.sdentj.2024.10.006","url":null,"abstract":"<div><h3>Introduction</h3><div>The World Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions introduced the 2018 Classification. Formulating periodontal diagnosis differs from clinician to clinician and depends on the expertise and competence of the clinician. We aimed to assess calibration levels of periodontal diagnosis based on the 2018 classification among senior-year dental interns at Batterjee Medical College in Saudi Arabia preparing for practice and subsequent graduation.</div></div><div><h3>Materials and Methods</h3><div>In this preapproved study, 52 senior-year dental students received a two-hour presentation on the 2018 classification by a single teacher, followed by a preliminary exam. One week later, the students were subjected to 12 study cases demonstrating a variety of periodontal and <em>peri</em>-implant disorders with gold standard management, followed by a post-test. Case scenarios with photographs, knowledge cognition, knowledge recall, and case scenarios were the four areas of both exams. Kappa statistics were computed to determine agreement. Statistical significance was set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>The total examination score was 12.69 ± 2.74 and 15.12 ± 2.99 (p = 0.001) for the pre- and post-tests, respectively. Furthermore, the difficulty factor and discrimination index increased by 12 % (p = 0.007) and 7 % (p = 0.244), respectively. Finally, the kappa statistic showed moderate agreement at 0.427 (p = 0.002).</div></div><div><h3>Conclusion</h3><div>Inter-rater agreement with the gold standard increased after training, and this improvement was fairly successful in providing graduating dental students with diagnostic abilities simultaneously with the emergence of a new classification system. A calibration procedure based on a simple feedback approach provided beneficial outcomes.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1553-1558"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of platelet-rich plasma on RANKL and IL-1 immunohistochemical expression in periodontitis-related bone cell proliferation and differentiation","authors":"Hana H. Mustafa ,&nbsp;Snur M.A. Hassan ,&nbsp;Sozan Ali Mohammed ,&nbsp;Mardin O. Mohammed ,&nbsp;Hadia Karim Zorab ,&nbsp;Hardi Fattah Marif","doi":"10.1016/j.sdentj.2024.11.011","DOIUrl":"10.1016/j.sdentj.2024.11.011","url":null,"abstract":"<div><h3>Background</h3><div>Platelet-rich plasma (PRP) is utilized as an autologous blood product to encourage bone regeneration. The receptor activator of nuclear factor-NB ligand (RANKL) is a key and central regulator of osteoclast homeostasis. A rat model of experimentally generated periodontitis was used to assess the impact of PRP preparation on the expression of the osteoclastogenic and pro-inflammatory markers respectively; RANKL and IL-1β.</div></div><div><h3>Material and Methods</h3><div>To induce periodontitis by silk ligature, thirty-six adult male <em>Sprague Dawleys</em> rats were used and they were allocated into three equal groups (n = 12): group I consisted of intact periodontal tissue, group II; rat-induced periodontitis without treatment by PRP, and group III of periodontitis <strong>+</strong> 10 µL PRP injection. The rats were sacrificed after both experiment durations (7 and 30 days), and the incisor teeth were fixed and decalcified in HCl for a day and in 10 % EDTA solution for eight weeks at room temperature then samples were processed for H&amp;E stain for bone healing scores and bone cells counting, and the samples were utilized by IHC for detection of both RANKL and IL-1β expression.</div></div><div><h3>Results</h3><div>The PRP enhanced the process of healing on days 7 and 30 showed (Score 10) vs. the control positive group that had a delay in alveolar bone regarded as (Score 4) significantly (P ≤ 0.05). The PRP group attenuated significantly (P ≤ 0.05) the alveolar bone loss by increasing the number of osteoblasts and declining the proliferation of osteoclast vs. the control positive group that revealed bone destruction due to rising osteoclast proliferation and decreasing the osteoblast proliferation significantly (P ≤ 0.05). PRP inhibited the IL-1β expression (score = 0) vs. the control positive group that showed moderate staining of positive cells detected in both inflammatory cells and endothelium (score = 4). Regarding the RANKL expression, the PRP reduced its expression in vs. the control positive group (score = 4 vs. 12 respectively).</div></div><div><h3>Conclusion</h3><div>PRP is an anabolic agent that enhances proliferation of osteoblast and inhibit the osteoclast differentiation by downregulation of IL-1β and RANKL.</div></div>","PeriodicalId":47246,"journal":{"name":"Saudi Dental Journal","volume":"36 12","pages":"Pages 1593-1600"},"PeriodicalIF":1.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143130126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}