Applied Microscopy最新文献

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Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules 大鼠小脑系统发育小叶中浦肯野细胞树突棘与平行纤维静脉曲张突触密度的差异
Applied Microscopy Pub Date : 2020-02-27 DOI: 10.1186/s42649-020-00027-6
Hyun-Wook Kim, Seung Hak Oh, Se Jeong Lee, Ji eun Na, Im Joo Rhyu
{"title":"Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules","authors":"Hyun-Wook Kim,&nbsp;Seung Hak Oh,&nbsp;Se Jeong Lee,&nbsp;Ji eun Na,&nbsp;Im Joo Rhyu","doi":"10.1186/s42649-020-00027-6","DOIUrl":"https://doi.org/10.1186/s42649-020-00027-6","url":null,"abstract":"<p>The cerebellum is a region of the brain that plays an important role in motor control. It is classified phylogenetically into archicerebellum, paleocerebellum and neocerebellum. The Purkinje cells are lined in a row called Purkinje cell layer and it has a unique dendritic branches with many spines.</p><p>The previous study reported that there is a difference of synapse density according to the lobules based on large two-dimensional data. However, recent study with high voltage electron microscopy showed there was no differences in dendritic spine density of the Purkinje cell according to its phylogenetic lobule. We analyzed Purkinje cell density in the II, VI and X lobules by stereological modules and synaptic density was estimated by double disector based on Purkinje cell density in the molecular layer of each lobule.</p><p>The results showed that there was significant difference in the Purkinje cell density and synapse number according to their phylogenetic lobules. The number of Purkinje cell in a given volume was larger in the archicerebellum, but synapse density was higher in the neocerebellum.</p><p>These data suggest that cellular and synaptic organization of the Purkinje cell is different according to their phylogenetic background.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00027-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5034762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Microstructural evolution of tantalum nitride thin films synthesized by inductively coupled plasma sputtering 电感耦合等离子溅射制备氮化钽薄膜的微观结构演变
Applied Microscopy Pub Date : 2020-02-27 DOI: 10.1186/s42649-020-00026-7
Sung-Il Baik, Young-Woon Kim
{"title":"Microstructural evolution of tantalum nitride thin films synthesized by inductively coupled plasma sputtering","authors":"Sung-Il Baik,&nbsp;Young-Woon Kim","doi":"10.1186/s42649-020-00026-7","DOIUrl":"https://doi.org/10.1186/s42649-020-00026-7","url":null,"abstract":"<p>Tantalum nitride (TaN<sub>x</sub>) thin films were grown utilizing an inductively coupled plasma (ICP) assisted direct current (DC) sputtering, and 20–100% improved microhardness values were obtained. The detailed microstructural changes of the TaN<sub>x</sub> films were characterized utilizing transmission electron microscopy (TEM), as a function of nitrogen gas fraction and ICP power. As nitrogen gas fraction increases?from 0.05 to 0.15, the TaN<sub>x</sub> phase evolves from body-centered-cubic (b.c.c.) TaN<sub>0.1</sub>, to face-centered-cubic (f.c.c.) δ-TaN, to hexagonal-close-packing (h.c.p.) ε-TaN phase. By increasing ICP power from 100?W to 400?W, the f.c.c. δ- TaN phase becomes the main phase in all nitrogen fractions investigated. The higher ICP power enhances the mobility of Ta and N ions, which stabilizes the δ-TaN phase like a high-temperature regime and removes the micro-voids between the columnar grains in the TaN<sub>x</sub> film. The dense δ-TaN structure with reduced columnar grains and micro-voids increases the strength of the TaN<sub>x</sub> film.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00026-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5035768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Microscopic analysis of metal matrix composites containing carbon Nanomaterials 含碳纳米材料金属基复合材料的微观分析
Applied Microscopy Pub Date : 2020-02-10 DOI: 10.1186/s42649-019-0024-2
Daeyoung Kim, Hye Jung Chang, Hyunjoo Choi
{"title":"Microscopic analysis of metal matrix composites containing carbon Nanomaterials","authors":"Daeyoung Kim,&nbsp;Hye Jung Chang,&nbsp;Hyunjoo Choi","doi":"10.1186/s42649-019-0024-2","DOIUrl":"https://doi.org/10.1186/s42649-019-0024-2","url":null,"abstract":"<p>Metallic matrix composites reinforced with carbon nanomaterials continue to attract interest because of their excellent mechanical, thermal, and electrical properties. However, two critical issues have limited their commercialization. Uniform distribution of carbon nanomaterials in metallic matrices is difficult, and the interfaces between the nanomaterials and matrices are weak. Microscope-based analysis was recently used to quantitatively examine these microstructural features and investigate their contributions to the composites’ mechanical, thermal, and electrical properties. The impacts of the microstructure on these properties are discussed in the first section of this review. In the second section, the various microscopic techniques used to study the distribution of carbon nanomaterials in metallic matrices and their interfaces are described.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0024-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4417905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Publisher Correction to: Applied Microscopy 出版商更正:应用显微镜
Applied Microscopy Pub Date : 2020-02-05 DOI: 10.1186/s42649-020-0025-1
Applied Microscopy
{"title":"Publisher Correction to: Applied Microscopy","authors":"Applied Microscopy","doi":"10.1186/s42649-020-0025-1","DOIUrl":"https://doi.org/10.1186/s42649-020-0025-1","url":null,"abstract":"","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-0025-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4207052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Introduction to the standard reference data of electron energy loss spectra and their database: eel.geri.re.kr 电子能量损失谱的标准参考数据及其数据库:eele .geri.re.kr
Applied Microscopy Pub Date : 2019-12-31 DOI: 10.1186/s42649-019-0015-3
Jeong Eun Chae, Ji-Soo Kim, Sang-Yeol Nam, Min Su Kim, Jucheol Park
{"title":"Introduction to the standard reference data of electron energy loss spectra and their database: eel.geri.re.kr","authors":"Jeong Eun Chae,&nbsp;Ji-Soo Kim,&nbsp;Sang-Yeol Nam,&nbsp;Min Su Kim,&nbsp;Jucheol Park","doi":"10.1186/s42649-019-0015-3","DOIUrl":"https://doi.org/10.1186/s42649-019-0015-3","url":null,"abstract":"<p>Electron energy loss spectroscopy (EELS) is an analytical technique that can provide the structural, physical and chemical information of materials. The EELS spectra can be obtained by combining with TEM at sub-nanometer spatial resolution. However, EELS spectral information can’t be obtained easily because in order to interpret EELS spectra, we need to refer to and/or compare many reference data with each other. And in addition to that, we should consider the different experimental variables used to produce each data. Therefore, reliable and easily interpretable EELS standard reference data are needed.</p><p>Our Electron Energy Loss Data Center (EELDC) has been designated as National Standard Electron Energy Loss Data Center No. 34 to develop EELS standard reference (SR) data and to play a role in dissemination and diffusion of the SR data to users. EELDC has developed and collected EEL SR data for the materials required by major industries and has a total of 82 EEL SR data. Also, we have created an online platform that provides a one-stop-place to help users interpret quickly EELS spectra and get various spectral information. In this paper, we introduce EEL SR data, the homepage of EELDC and how to use them.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0015-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5172509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Microscopic study of zinc nanoparticles synthesised using thermosetting polymer 热固性聚合物合成锌纳米颗粒的显微研究
Applied Microscopy Pub Date : 2019-12-31 DOI: 10.1186/s42649-019-0018-0
Giriraj Tailor, Jyoti Chaudhay, Deepshikha Verma, Bhupendra Kr. Sarma
{"title":"Microscopic study of zinc nanoparticles synthesised using thermosetting polymer","authors":"Giriraj Tailor,&nbsp;Jyoti Chaudhay,&nbsp;Deepshikha Verma,&nbsp;Bhupendra Kr. Sarma","doi":"10.1186/s42649-019-0018-0","DOIUrl":"https://doi.org/10.1186/s42649-019-0018-0","url":null,"abstract":"<p>The present study reports the novel synthesis of Zinc nanoparticles (Zn NPs) by thermal decomposition method and its characterisation by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), and X-ray Diffraction Measurements (XRD). Synthesis of Zn NPs was achieved by using thermosetting polymer and zinc salts as precursor. Zn NPs were obtained on calcination at 850?°C for 30?min. SEM study reveals that synthesized nanoparticles are spherical in shape. XRD analysis shows that the Zn NPs formed are low crystalline in nature.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0018-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5173576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Composite cuticle with heterogeneous layers in the leaf epidermis of Ficus elastica 弹性榕树叶表皮具有异质层的复合角质层
Applied Microscopy Pub Date : 2019-12-24 DOI: 10.1186/s42649-019-0022-4
Ki Woo Kim
{"title":"Composite cuticle with heterogeneous layers in the leaf epidermis of Ficus elastica","authors":"Ki Woo Kim","doi":"10.1186/s42649-019-0022-4","DOIUrl":"https://doi.org/10.1186/s42649-019-0022-4","url":null,"abstract":"<p>Two distinct layers in terms of texture and electron density were observed in the leaf cuticle of <i>Ficus elastica</i> using transmission electron microscopy. As depicted in a model, an inner polysaccharide-rich layer and an outer cutin (or cutan)-rich layer may support the composite, heterogeneous concept of the leaf cuticle.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0022-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4925385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Anatomy, ultrastructure and histology of the olfactory organ of the largemouth bass Micropterus salmoides, Centrarchidae 大口黑鲈(Micropterus salmoides, Centrarchidae)嗅器官解剖、超微结构和组织学
Applied Microscopy Pub Date : 2019-12-23 DOI: 10.1186/s42649-019-0023-3
Hyun Tae Kim, Seung Woon Yun, Jong Young Park
{"title":"Anatomy, ultrastructure and histology of the olfactory organ of the largemouth bass Micropterus salmoides, Centrarchidae","authors":"Hyun Tae Kim,&nbsp;Seung Woon Yun,&nbsp;Jong Young Park","doi":"10.1186/s42649-019-0023-3","DOIUrl":"https://doi.org/10.1186/s42649-019-0023-3","url":null,"abstract":"<p>The detailed anatomy, ultrastructure and histology of the olfactory organ of <i>Micropterus salmoides</i> were investigated by a stereo microscope, a light microscope, and a scanning electron microscope. Its external structure shows a tube-like anterior nostril to stick out and a posterior nostril flat to the skin surface. Meanwhile, its internal structure, the olfactory chamber, contains a fan-shaped rosette structure with 9 to 11 lamellae in adult fish over 35?cm in standard length (SL) and two accessory nasal sacs (ethmoidal and lacrimal sacs) were found. Interestingly, the rosette in young fish under 15?cm in SL was a longitudinal structure in parallel with each of 4–5 lamellae. Histologically, the sensory epithelium (SE) on the olfactory chamber consists of 5 types of cells: olfactory receptor neurons, supporting cells, basal cells, lymphatic cells and mucous cells. In contrast, the non-sensory epithelium (NSE) has stratified epithelial cells, lymphatic cells and mucous cells. The mucous cells of the SE are abundant and distributed densely in one row on the outermost superficial surface, but the one of the NSE are less than the SE. From these results, the olfactory characters of <i>M. salmoides</i> may be related with its ecological habit spending in the middle layer of stagnant water contaminated, more or less.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0023-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5189630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Formation of two-dimensional MoS2 and one-dimensional MoO2 nanowire hybrids 二维MoS2和一维MoO2纳米线杂化的形成
Applied Microscopy Pub Date : 2019-12-17 DOI: 10.1186/s42649-019-0020-6
Aram Yoon, Zonghoon Lee
{"title":"Formation of two-dimensional MoS2 and one-dimensional MoO2 nanowire hybrids","authors":"Aram Yoon,&nbsp;Zonghoon Lee","doi":"10.1186/s42649-019-0020-6","DOIUrl":"https://doi.org/10.1186/s42649-019-0020-6","url":null,"abstract":"<p>Oxidation of two-dimensional (2D) transition metal dichalcogenides have received great interests because it significantly influences their electrical, optical, and catalytic properties. Monoclinic MoO<sub>2</sub> nanowires grow along the zigzag direction of 2D MoS<sub>2</sub> via thermal annealing at a high temperature with a low oxygen partial pressure. The hybrids of semiconducting 2D MoS<sub>2</sub> and metallic 1D MoO<sub>2</sub> nanowires have potential to be applied to various devices such as electrical devices, gas sensors, photodetectors, and catalysts.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0020-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4671633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electron beam irradiation induced crystallization behavior of amorphous Ge2Sb2Te5 chalcogenide material 电子束辐照诱导非晶态Ge2Sb2Te5硫系材料的结晶行为
Applied Microscopy Pub Date : 2019-12-17 DOI: 10.1186/s42649-019-0021-5
Byeong-Seon An
{"title":"Electron beam irradiation induced crystallization behavior of amorphous Ge2Sb2Te5 chalcogenide material","authors":"Byeong-Seon An","doi":"10.1186/s42649-019-0021-5","DOIUrl":"https://doi.org/10.1186/s42649-019-0021-5","url":null,"abstract":"<p>The crystallization of amorphous Ge<sub>2</sub>Sb<sub>2</sub>Te<sub>5</sub> phase change material induced by electron beam irradiation was investigated by in-situ transmission electron microscopy (TEM). Amorphous matrix transformed into a partially crystalline state after being irradiated with a 200-keV electron beam for a long time. Real-time observation revealed that the crystallization of amorphous Ge<sub>2</sub>Sb<sub>2</sub>Te<sub>5</sub> film occurs through a nucleation and growth mechanism under electron beam irradiation in TEM. While uncertainty from the 2D projection remains, the nuclei have been observed to grow preferentially along the &lt;?100&gt; direction.</p>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0021-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4672090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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