Applied Microscopy最新文献_第9页

Hot stage microscopy and its applications in pharmaceutical characterization 热阶显微镜及其在药物表征中的应用

Applied Microscopy Pub Date : 2020-06-16 DOI: 10.1186/s42649-020-00032-9

Arun Kumar, Pritam Singh, Arun Nanda

{"title":"Hot stage microscopy and its applications in pharmaceutical characterization","authors":"Arun Kumar, Pritam Singh, Arun Nanda","doi":"10.1186/s42649-020-00032-9","DOIUrl":"https://doi.org/10.1186/s42649-020-00032-9","url":null,"abstract":"Hot stage microscopy (HSM) is a thermal analysis technique that combines the best properties of thermal analysis and microscopy. HSM is rapidly gaining interest in pharmaceuticals as well as in other fields as a regular characterization technique. In pharmaceuticals HSM is used to support differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA) observations and to detect small changes in the sample that may be missed by DSC and TGA during a thermal experiment. Study of various physical and chemical properties such sample morphology, crystalline nature, polymorphism, desolvation, miscibility, melting, solid state transitions and incompatibility between various pharmaceutical compounds can be carried out using HSM. HSM is also widely used to screen cocrystals, excipients and polymers for solid dispersions. With the advancements in research methodologies, it is now possible to use HSM in conjunction with other characterization techniques such as Fourier transform infrared spectroscopy (FTIR), DSC, Raman spectroscopy, scanning electron microscopy (SEM) which may have additional benefits over traditional characterization techniques for rapid and comprehensive solid state characterization.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00032-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4650886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse 移植的下鼻甲来源的干细胞在小鼠退化视网膜中向杆状光感受器分化的光镜证据

Applied Microscopy Pub Date : 2020-06-03 DOI: 10.1186/s42649-020-00031-w

Yong Soo Park, Yeonji Kim, Sung Won Kim, In-Beom Kim

引用次数: 0

Methanol fixation for scanning electron microscopy of plants 植物扫描电镜甲醇固定

Applied Microscopy Pub Date : 2020-05-25 DOI: 10.1186/s42649-020-00028-5

Ki Woo Kim

引用次数: 1

Fine structure of the cardiac muscle cells in the orb-web spider Nephila clavata 圆网蜘蛛心肌细胞的精细结构

Applied Microscopy Pub Date : 2020-05-14 DOI: 10.1186/s42649-020-00030-x

Yan Sun, Hyo-Jeong Kim, Myung-Jin Moon

{"title":"Fine structure of the cardiac muscle cells in the orb-web spider Nephila clavata","authors":"Yan Sun, Hyo-Jeong Kim, Myung-Jin Moon","doi":"10.1186/s42649-020-00030-x","DOIUrl":"https://doi.org/10.1186/s42649-020-00030-x","url":null,"abstract":"The fine structural characteristics of cardiac muscle cells and its myofibril organization in the orb web spider N. clavata were examined by transmission electron microscopy. Although myofibril striations are not remarkable as those of skeletal muscles, muscle fibers contain multiple myofibrils, abundant mitochondria, extensive sarcoplasmic reticulum and transverse tubules (T-tubules). Myofibrils are divided into distinct sarcomeres defined by Z-lines with average length of 2.0?μm, but the distinction between the A-band and the I-bands is not clear due to uniform striations over the length of the sarcomeres. Dyadic junction which consisted of a single T-tubule paired with a terminal cisterna of the sarcoplasmic reticulum is found mainly at the A-I level of sarcomere. Each cell is arranged to form multiple connections with neighboring cells through the intercalated discs. These specialized junctions include three types of intercellular junctions: gap junctions, fascia adherens and desmosomes for heart function. Our transmission electron microscopy (TEM) observations clearly show that spider’s cardiac muscle contraction is controlled by neurogenic rather than myogenic mechanism since each cardiac muscle fiber is innervated by a branch of motor neuron through neuromuscular junctions.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00030-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4583745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative study on the specimen thickness measurement using EELS and CBED methods EELS法与CBED法测量试件厚度的对比研究

Applied Microscopy Pub Date : 2020-05-12 DOI: 10.1186/s42649-020-00029-4

Yoon-Uk Heo

引用次数: 10

Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules 大鼠小脑系统发育小叶中浦肯野细胞树突棘与平行纤维静脉曲张突触密度的差异

Applied Microscopy Pub Date : 2020-02-27 DOI: 10.1186/s42649-020-00027-6

Hyun-Wook Kim, Seung Hak Oh, Se Jeong Lee, Ji eun Na, Im Joo Rhyu

{"title":"Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules","authors":"Hyun-Wook Kim, Seung Hak Oh, Se Jeong Lee, Ji eun Na, Im Joo Rhyu","doi":"10.1186/s42649-020-00027-6","DOIUrl":"https://doi.org/10.1186/s42649-020-00027-6","url":null,"abstract":"The cerebellum is a region of the brain that plays an important role in motor control. It is classified phylogenetically into archicerebellum, paleocerebellum and neocerebellum. The Purkinje cells are lined in a row called Purkinje cell layer and it has a unique dendritic branches with many spines.The previous study reported that there is a difference of synapse density according to the lobules based on large two-dimensional data. However, recent study with high voltage electron microscopy showed there was no differences in dendritic spine density of the Purkinje cell according to its phylogenetic lobule. We analyzed Purkinje cell density in the II, VI and X lobules by stereological modules and synaptic density was estimated by double disector based on Purkinje cell density in the molecular layer of each lobule.The results showed that there was significant difference in the Purkinje cell density and synapse number according to their phylogenetic lobules. The number of Purkinje cell in a given volume was larger in the archicerebellum, but synapse density was higher in the neocerebellum.These data suggest that cellular and synaptic organization of the Purkinje cell is different according to their phylogenetic background.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00027-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5034762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Microstructural evolution of tantalum nitride thin films synthesized by inductively coupled plasma sputtering 电感耦合等离子溅射制备氮化钽薄膜的微观结构演变

Applied Microscopy Pub Date : 2020-02-27 DOI: 10.1186/s42649-020-00026-7

Sung-Il Baik, Young-Woon Kim

引用次数: 4

Microscopic analysis of metal matrix composites containing carbon Nanomaterials 含碳纳米材料金属基复合材料的微观分析

Applied Microscopy Pub Date : 2020-02-10 DOI: 10.1186/s42649-019-0024-2

Daeyoung Kim, Hye Jung Chang, Hyunjoo Choi

引用次数: 1

Publisher Correction to: Applied Microscopy 出版商更正:应用显微镜

Applied Microscopy Pub Date : 2020-02-05 DOI: 10.1186/s42649-020-0025-1

Applied Microscopy

引用次数: 0

Introduction to the standard reference data of electron energy loss spectra and their database: eel.geri.re.kr 电子能量损失谱的标准参考数据及其数据库:eele .geri.re.kr

Applied Microscopy Pub Date : 2019-12-31 DOI: 10.1186/s42649-019-0015-3

Jeong Eun Chae, Ji-Soo Kim, Sang-Yeol Nam, Min Su Kim, Jucheol Park

{"title":"Introduction to the standard reference data of electron energy loss spectra and their database: eel.geri.re.kr","authors":"Jeong Eun Chae, Ji-Soo Kim, Sang-Yeol Nam, Min Su Kim, Jucheol Park","doi":"10.1186/s42649-019-0015-3","DOIUrl":"https://doi.org/10.1186/s42649-019-0015-3","url":null,"abstract":"Electron energy loss spectroscopy (EELS) is an analytical technique that can provide the structural, physical and chemical information of materials. The EELS spectra can be obtained by combining with TEM at sub-nanometer spatial resolution. However, EELS spectral information can’t be obtained easily because in order to interpret EELS spectra, we need to refer to and/or compare many reference data with each other. And in addition to that, we should consider the different experimental variables used to produce each data. Therefore, reliable and easily interpretable EELS standard reference data are needed.Our Electron Energy Loss Data Center (EELDC) has been designated as National Standard Electron Energy Loss Data Center No. 34 to develop EELS standard reference (SR) data and to play a role in dissemination and diffusion of the SR data to users. EELDC has developed and collected EEL SR data for the materials required by major industries and has a total of 82 EEL SR data. Also, we have created an online platform that provides a one-stop-place to help users interpret quickly EELS spectra and get various spectral information. In this paper, we introduce EEL SR data, the homepage of EELDC and how to use them.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-019-0015-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5172509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3