Applied Microscopy最新文献_第8页

Double staining method for array tomography using scanning electron microscopy 扫描电子显微镜阵列层析成像的双重染色方法

Applied Microscopy Pub Date : 2020-06-22 DOI: 10.1186/s42649-020-00033-8

Eunjin Kim, Jiyoung Lee, Seulgi Noh, Ohkyung Kwon, Ji Young Mun

引用次数: 2

Ultrastructure of the fertilized egg envelopes in Ancistrus cirrhosus, Loricariidae, Teleostei 硬腹蛇、蠓科、铁骨鱼受精卵包膜的超微结构

Applied Microscopy Pub Date : 2020-06-17 DOI: 10.1186/s42649-020-00034-7

Dong Heui Kim

{"title":"Ultrastructure of the fertilized egg envelopes in Ancistrus cirrhosus, Loricariidae, Teleostei","authors":"Dong Heui Kim","doi":"10.1186/s42649-020-00034-7","DOIUrl":"https://doi.org/10.1186/s42649-020-00034-7","url":null,"abstract":"We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15–19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58?±?0.85?μm (n?=?20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00034-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4687014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Hot stage microscopy and its applications in pharmaceutical characterization 热阶显微镜及其在药物表征中的应用

Applied Microscopy Pub Date : 2020-06-16 DOI: 10.1186/s42649-020-00032-9

Arun Kumar, Pritam Singh, Arun Nanda

{"title":"Hot stage microscopy and its applications in pharmaceutical characterization","authors":"Arun Kumar, Pritam Singh, Arun Nanda","doi":"10.1186/s42649-020-00032-9","DOIUrl":"https://doi.org/10.1186/s42649-020-00032-9","url":null,"abstract":"Hot stage microscopy (HSM) is a thermal analysis technique that combines the best properties of thermal analysis and microscopy. HSM is rapidly gaining interest in pharmaceuticals as well as in other fields as a regular characterization technique. In pharmaceuticals HSM is used to support differential scanning calorimetry (DSC) and thermo-gravimetric analysis (TGA) observations and to detect small changes in the sample that may be missed by DSC and TGA during a thermal experiment. Study of various physical and chemical properties such sample morphology, crystalline nature, polymorphism, desolvation, miscibility, melting, solid state transitions and incompatibility between various pharmaceutical compounds can be carried out using HSM. HSM is also widely used to screen cocrystals, excipients and polymers for solid dispersions. With the advancements in research methodologies, it is now possible to use HSM in conjunction with other characterization techniques such as Fourier transform infrared spectroscopy (FTIR), DSC, Raman spectroscopy, scanning electron microscopy (SEM) which may have additional benefits over traditional characterization techniques for rapid and comprehensive solid state characterization.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00032-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4650886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Light microscopic evidence of in vivo differentiation from the transplanted inferior turbinate-derived stem cell into the rod photoreceptor in degenerating retina of the mouse 移植的下鼻甲来源的干细胞在小鼠退化视网膜中向杆状光感受器分化的光镜证据

Applied Microscopy Pub Date : 2020-06-03 DOI: 10.1186/s42649-020-00031-w

Yong Soo Park, Yeonji Kim, Sung Won Kim, In-Beom Kim

引用次数: 0

Methanol fixation for scanning electron microscopy of plants 植物扫描电镜甲醇固定

Applied Microscopy Pub Date : 2020-05-25 DOI: 10.1186/s42649-020-00028-5

Ki Woo Kim

引用次数: 1

Fine structure of the cardiac muscle cells in the orb-web spider Nephila clavata 圆网蜘蛛心肌细胞的精细结构

Applied Microscopy Pub Date : 2020-05-14 DOI: 10.1186/s42649-020-00030-x

Yan Sun, Hyo-Jeong Kim, Myung-Jin Moon

{"title":"Fine structure of the cardiac muscle cells in the orb-web spider Nephila clavata","authors":"Yan Sun, Hyo-Jeong Kim, Myung-Jin Moon","doi":"10.1186/s42649-020-00030-x","DOIUrl":"https://doi.org/10.1186/s42649-020-00030-x","url":null,"abstract":"The fine structural characteristics of cardiac muscle cells and its myofibril organization in the orb web spider N. clavata were examined by transmission electron microscopy. Although myofibril striations are not remarkable as those of skeletal muscles, muscle fibers contain multiple myofibrils, abundant mitochondria, extensive sarcoplasmic reticulum and transverse tubules (T-tubules). Myofibrils are divided into distinct sarcomeres defined by Z-lines with average length of 2.0?μm, but the distinction between the A-band and the I-bands is not clear due to uniform striations over the length of the sarcomeres. Dyadic junction which consisted of a single T-tubule paired with a terminal cisterna of the sarcoplasmic reticulum is found mainly at the A-I level of sarcomere. Each cell is arranged to form multiple connections with neighboring cells through the intercalated discs. These specialized junctions include three types of intercellular junctions: gap junctions, fascia adherens and desmosomes for heart function. Our transmission electron microscopy (TEM) observations clearly show that spider’s cardiac muscle contraction is controlled by neurogenic rather than myogenic mechanism since each cardiac muscle fiber is innervated by a branch of motor neuron through neuromuscular junctions.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00030-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4583745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparative study on the specimen thickness measurement using EELS and CBED methods EELS法与CBED法测量试件厚度的对比研究

Applied Microscopy Pub Date : 2020-05-12 DOI: 10.1186/s42649-020-00029-4

Yoon-Uk Heo

引用次数: 10

Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules 大鼠小脑系统发育小叶中浦肯野细胞树突棘与平行纤维静脉曲张突触密度的差异

Applied Microscopy Pub Date : 2020-02-27 DOI: 10.1186/s42649-020-00027-6

Hyun-Wook Kim, Seung Hak Oh, Se Jeong Lee, Ji eun Na, Im Joo Rhyu

{"title":"Differential synapse density between Purkinje cell dendritic spine and parallel fiber varicosity in the rat cerebellum among the phylogenic lobules","authors":"Hyun-Wook Kim, Seung Hak Oh, Se Jeong Lee, Ji eun Na, Im Joo Rhyu","doi":"10.1186/s42649-020-00027-6","DOIUrl":"https://doi.org/10.1186/s42649-020-00027-6","url":null,"abstract":"The cerebellum is a region of the brain that plays an important role in motor control. It is classified phylogenetically into archicerebellum, paleocerebellum and neocerebellum. The Purkinje cells are lined in a row called Purkinje cell layer and it has a unique dendritic branches with many spines.The previous study reported that there is a difference of synapse density according to the lobules based on large two-dimensional data. However, recent study with high voltage electron microscopy showed there was no differences in dendritic spine density of the Purkinje cell according to its phylogenetic lobule. We analyzed Purkinje cell density in the II, VI and X lobules by stereological modules and synaptic density was estimated by double disector based on Purkinje cell density in the molecular layer of each lobule.The results showed that there was significant difference in the Purkinje cell density and synapse number according to their phylogenetic lobules. The number of Purkinje cell in a given volume was larger in the archicerebellum, but synapse density was higher in the neocerebellum.These data suggest that cellular and synaptic organization of the Purkinje cell is different according to their phylogenetic background.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00027-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5034762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Microstructural evolution of tantalum nitride thin films synthesized by inductively coupled plasma sputtering 电感耦合等离子溅射制备氮化钽薄膜的微观结构演变

Applied Microscopy Pub Date : 2020-02-27 DOI: 10.1186/s42649-020-00026-7

Sung-Il Baik, Young-Woon Kim

引用次数: 4

Microscopic analysis of metal matrix composites containing carbon Nanomaterials 含碳纳米材料金属基复合材料的微观分析

Applied Microscopy Pub Date : 2020-02-10 DOI: 10.1186/s42649-019-0024-2

Daeyoung Kim, Hye Jung Chang, Hyunjoo Choi

引用次数: 1