Applied Microscopy最新文献_第8页

Method of Ga removal from a specimen on a microelectromechanical system-based chip for in-situ transmission electron microscopy 用于原位透射电子显微镜的基于微机电系统芯片的样品Ga去除方法

Applied Microscopy Pub Date : 2020-10-14 DOI: 10.1186/s42649-020-00043-6

Yena Kwon, Byeong-Seon An, Yeon-Ju Shin, Cheol-Woong Yang

{"title":"Method of Ga removal from a specimen on a microelectromechanical system-based chip for in-situ transmission electron microscopy","authors":"Yena Kwon, Byeong-Seon An, Yeon-Ju Shin, Cheol-Woong Yang","doi":"10.1186/s42649-020-00043-6","DOIUrl":"https://doi.org/10.1186/s42649-020-00043-6","url":null,"abstract":"In-situ transmission electron microscopy (TEM) holders that employ a chip-type specimen stage have been widely utilized in recent years. The specimen on the microelectromechanical system (MEMS)-based chip is commonly prepared by focused ion beam (FIB) milling and ex-situ lift-out (EXLO). However, the FIB-milled thin-foil specimens are inevitably contaminated with Ga+ ions. When these specimens are heated for real time observation, the Ga+ ions influence the reaction or aggregate in the protection layer. An effective method of removing the Ga residue by Ar+ ion milling within FIB system was explored in this study. However, the Ga residue remained in the thin-foil specimen that was extracted by EXLO from the trench after the conduct of Ar+ ion milling. To address this drawback, the thin-foil specimen was attached to an FIB lift-out grid, subjected to Ar+ ion milling, and subsequently transferred to an MEMS-based chip by EXLO. The removal of the Ga residue was confirmed by energy dispersive spectroscopy.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00043-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4591742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sublimable materials facilitate the TEM sample preparation of oil-soluble nanomaterials 可升华材料有利于油溶性纳米材料的TEM样品制备

Applied Microscopy Pub Date : 2020-09-29 DOI: 10.1186/s42649-020-00042-7

Yu-Hao Deng

引用次数: 1

Fine structure of the intercalated disc and cardiac junctions in the black widow spider Latrodectus mactans 黑寡妇蜘蛛间插盘和心脏连接处的精细结构

Applied Microscopy Pub Date : 2020-09-25 DOI: 10.1186/s42649-020-00040-9

Yan Sun, Seung-Min Lee, Bon-Jin Ku, Myung-Jin Moon

{"title":"Fine structure of the intercalated disc and cardiac junctions in the black widow spider Latrodectus mactans","authors":"Yan Sun, Seung-Min Lee, Bon-Jin Ku, Myung-Jin Moon","doi":"10.1186/s42649-020-00040-9","DOIUrl":"https://doi.org/10.1186/s42649-020-00040-9","url":null,"abstract":"Arthropods have an open circulatory system with a simple tubular heart, so it has been estimated that the contractile pumping structure of the cardiac muscle will be less efficient than that of vertebrates. Nevertheless, certain arthropods are known to have far superior properties and characteristics than vertebrates, so we investigated the fine structural features of intercalated discs and cardiac junctions of cardiac muscle cells in the black widow spider Latrodectus mactans. Characteristically, the spider cardiac muscle has typical striated features and represents a functional syncytium that supports multiple connections to adjacent cells by intercalated discs. Histologically, the boundary lamina of each sarcolemma connects to the basement membrane to form an elastic sheath, and the extracellular matrix allows the cells to be anchored to other tissues. Since the intercalated disc is also part of sarcolemma, it contains gap junctions for depolarization and desmosomes that keep the fibers together during cardiac muscle contraction. Furthermore, fascia adherens and macula adherens (desmosomes) were also identified as cell junctions in both sarcolemma and intercalated discs. To enable the coordinated heartbeat of the cardiac muscle, the muscle fibers have neuronal innervations by multiple axons from the motor ganglion.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00040-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5382339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Methods to evaluate the twin formation energy: comparative studies of the atomic simulations and in-situ TEM tensile tests 评价孪晶形成能的方法:原子模拟与原位TEM拉伸试验的对比研究

Applied Microscopy Pub Date : 2020-09-17 DOI: 10.1186/s42649-020-00039-2

Hong-Kyu Kim, Sung-Hoon Kim, Jae-Pyoung Ahn

引用次数: 1

Microscopic research on the olfactory organ of the Far Eastern brook lamprey Lethenteron reissneri (Pisces, Petromyzontidae) 远东七鳃鳗嗅觉器官的显微研究(双鱼座，七鳃鳗科)

Applied Microscopy Pub Date : 2020-09-15 DOI: 10.1186/s42649-020-00038-3

Hyun-Tae Kim, Jong-Young Park

{"title":"Microscopic research on the olfactory organ of the Far Eastern brook lamprey Lethenteron reissneri (Pisces, Petromyzontidae)","authors":"Hyun-Tae Kim, Jong-Young Park","doi":"10.1186/s42649-020-00038-3","DOIUrl":"https://doi.org/10.1186/s42649-020-00038-3","url":null,"abstract":"The olfactory anatomy and histology of Lethenteron reissneri were researched using a stereo microscope, a light microscope, and a scanning electron microscope. As in other lampreys, it shows same characters as follows: i) a single olfactory organ, ii) a single tubular nostril, iii) a single olfactory chamber with gourd-like form, iv) a nasal valve, v) a nasopharyngeal pouch, vi) a sensory epithelium (SE) of continuous distribution, vii) a supporting cells with numerous long cilia, viii) an accessory olfactory organ. However, the description of a pseudostratified columnar layer in the SE and Non SE is a first record, not reported in sea lamprey Petromyzon marinus. In particular, both 19 to 20 lamellae in number and olfactory receptor neuron’s quarter ciliary length of the knob diameter differ from those of P. marinus. From these results, it might be considered that the olfactory organ of L. reissneri shows well adaptive structure of a primitive fish to slow flowing water with gravel, pebbles, and sand and a hiding habit into sand bottom at daytime. The lamellar number and neuron’s ciliary length may be a meaningful taxonomic character for the class Petromyzonida.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00038-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4922322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Depth-dependent EBIC microscopy of radial-junction Si micropillar arrays 径向结硅微柱阵列的深度依赖EBIC显微镜

Applied Microscopy Pub Date : 2020-09-03 DOI: 10.1186/s42649-020-00037-4

Kaden M. Powell, Heayoung P. Yoon

{"title":"Depth-dependent EBIC microscopy of radial-junction Si micropillar arrays","authors":"Kaden M. Powell, Heayoung P. Yoon","doi":"10.1186/s42649-020-00037-4","DOIUrl":"https://doi.org/10.1186/s42649-020-00037-4","url":null,"abstract":"Recent advances in fabrication have enabled radial-junction architectures for cost-effective and high-performance optoelectronic devices. Unlike a planar PN junction, a radial-junction geometry maximizes the optical interaction in the three-dimensional (3D) structures, while effectively extracting the generated carriers via the conformal PN junction. In this paper, we report characterizations of radial PN junctions that consist of p-type Si micropillars created by deep reactive-ion etching (DRIE) and an n-type layer formed by phosphorus gas diffusion. We use electron-beam induced current (EBIC) microscopy to access the 3D junction profile from the sidewall of the pillars. Our EBIC images reveal uniform PN junctions conformally constructed on the 3D pillar array. Based on Monte-Carlo simulations and EBIC modeling, we estimate local carrier separation/collection efficiency that reflects the quality of the PN junction. We find the EBIC efficiency of the pillar array increases with the incident electron beam energy, consistent with the EBIC behaviors observed in a high-quality planar PN junction. The magnitude of the EBIC efficiency of our pillar array is about 70% at 10?kV, slightly lower than that of the planar device (≈ 81%). We suggest that this reduction could be attributed to the unpassivated pillar surface and the unintended recombination centers in the pillar cores introduced during the DRIE processes. Our results support that the depth-dependent EBIC approach is ideally suitable for evaluating PN junctions formed on micro/nanostructured semiconductors with various geometry.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00037-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4145671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Fine structure of the silk spinning system in the caddisworm, Hydatophylax nigrovittatus (Trichoptera: Limnephilidae) 黑头棘球虫丝系统的精细结构(毛翅目:织丝虫科)

Applied Microscopy Pub Date : 2020-08-06 DOI: 10.1186/s42649-020-00036-5

Hyo-Jeong Kim, Yan Sun, Myung-Jin Moon

{"title":"Fine structure of the silk spinning system in the caddisworm, Hydatophylax nigrovittatus (Trichoptera: Limnephilidae)","authors":"Hyo-Jeong Kim, Yan Sun, Myung-Jin Moon","doi":"10.1186/s42649-020-00036-5","DOIUrl":"https://doi.org/10.1186/s42649-020-00036-5","url":null,"abstract":"Silk is produced by a variety of insects, but only silk made by terrestrial arthropods has been examined in detail. To fill the gap, this study was designed to understand the silk spinning system of aquatic insect. The larvae of caddis flies, Hydatophylax nigrovittatus produce silk through a pair of labial silk glands and use raw silk to protect themselves in the aquatic environment. The result of this study clearly shows that although silk fibers are made under aquatic conditions, the cellular silk production system is quite similar to that of terrestrial arthropods. Typically, silk production in caddisworm has been achieved by two independent processes in the silk glands. This includes the synthesis of silk fibroin in the posterior region, the production of adhesive glycoproteins in the anterior region, which are ultimately accumulated into functional silk dope and converted to a silk ribbon coated with gluey substances. At the cellular level, each substance of fibroin and glycoprotein is specifically synthesized at different locations, and then transported from the rough ER to the Golgi apparatus as transport vesicles, respectively. Thereafter, the secretory vesicles gradually increase in size by vesicular fusion, forming larger secretory granules containing specific proteins. It was found that these granules eventually migrate to the apical membrane and are exocytosed into the lumen by a mechanism of merocrine secretion.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00036-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4238184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Replacing critical point drying with a low-cost chemical drying provides comparable surface image quality of glandular trichomes from leaves of Millingtonia hortensis L. f. in scanning electron micrograph 用低成本的化学干燥法代替临界点干燥法，可以获得与之相当的扫描电镜图像质量

Applied Microscopy Pub Date : 2020-07-17 DOI: 10.1186/s42649-020-00035-6

Raktim Bhattacharya, Sulagna Saha, Olga Kostina, Lyudmila Muravnik, Adinpunya Mitra

引用次数: 18

Double staining method for array tomography using scanning electron microscopy 扫描电子显微镜阵列层析成像的双重染色方法

Applied Microscopy Pub Date : 2020-06-22 DOI: 10.1186/s42649-020-00033-8

Eunjin Kim, Jiyoung Lee, Seulgi Noh, Ohkyung Kwon, Ji Young Mun

引用次数: 2

Ultrastructure of the fertilized egg envelopes in Ancistrus cirrhosus, Loricariidae, Teleostei 硬腹蛇、蠓科、铁骨鱼受精卵包膜的超微结构

Applied Microscopy Pub Date : 2020-06-17 DOI: 10.1186/s42649-020-00034-7

Dong Heui Kim

{"title":"Ultrastructure of the fertilized egg envelopes in Ancistrus cirrhosus, Loricariidae, Teleostei","authors":"Dong Heui Kim","doi":"10.1186/s42649-020-00034-7","DOIUrl":"https://doi.org/10.1186/s42649-020-00034-7","url":null,"abstract":"We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15–19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58?±?0.85?μm (n?=?20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00034-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4687014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3