Applied Microscopy最新文献_第7页

Sequential conversion from line defects to atomic clusters in monolayer WS2 单层WS2中从线缺陷到原子簇的顺序转换

Applied Microscopy Pub Date : 2020-11-30 DOI: 10.1186/s42649-020-00047-2

Gyeong Hee Ryu, Ren-Jie Chan

引用次数: 1

Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images 利用高分辨率扫描电镜图像分析了干细胞在纳米柱周期性阵列上的行为

Applied Microscopy Pub Date : 2020-11-17 DOI: 10.1186/s42649-020-00046-3

Jihun Kang, Eun-Hye Kang, Young-Shik Yun, Seungmuk Ji, In-Sik Yun, Jong-Souk Yeo

引用次数: 0

Scanning acoustic microscopy for material evaluation 用于材料评价的扫描声学显微镜

Applied Microscopy Pub Date : 2020-11-05 DOI: 10.1186/s42649-020-00045-4

Hyunung Yu

{"title":"Scanning acoustic microscopy for material evaluation","authors":"Hyunung Yu","doi":"10.1186/s42649-020-00045-4","DOIUrl":"https://doi.org/10.1186/s42649-020-00045-4","url":null,"abstract":"Scanning acoustic microscopy (SAM) or Acoustic Micro Imaging (AMI) is a powerful, non-destructive technique that can detect hidden defects in elastic and biological samples as well as non-transparent hard materials. By monitoring the internal features of a sample in three-dimensional integration, this technique can efficiently find physical defects such as cracks, voids, and delamination with high sensitivity. In recent years, advanced techniques such as ultrasound impedance microscopy, ultrasound speed microscopy, and scanning acoustic gigahertz microscopy have been developed for applications in industries and in the medical field to provide additional information on the internal stress, viscoelastic, and anisotropic, or nonlinear properties. X-ray, magnetic resonance, and infrared techniques are the other competitive and widely used methods. However, they have their own advantages and limitations owing to their inherent properties such as different light sources and sensors.This paper provides an overview of the principle of SAM and presents a few results to demonstrate the applications of modern acoustic imaging technology. A variety of inspection modes, such as vertical, horizontal, and diagonal cross-sections have been presented by employing the focus pathway and image reconstruction algorithm. Images have been reconstructed from the reflected echoes resulting from the change in the acoustic impedance at the interface of the material layers or defects. The results described in this paper indicate that the novel acoustic technology can expand the scope of SAM as a versatile diagnostic tool requiring less time and having a high efficiency.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00045-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4226738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

The LaserFIB: new application opportunities combining a high-performance FIB-SEM with femtosecond laser processing in an integrated second chamber LaserFIB:在集成的第二腔室中，将高性能FIB-SEM与飞秒激光处理相结合，提供了新的应用机会

Applied Microscopy Pub Date : 2020-10-26 DOI: 10.1186/s42649-020-00044-5

Ben Tordoff, Cheryl Hartfield, Andrew J. Holwell, Stephan Hiller, Marcus Kaestner, Stephen Kelly, Jaehan Lee, Sascha Müller, Fabian Perez-Willard, Tobias Volkenandt, Robin White, Thomas Rodgers

{"title":"The LaserFIB: new application opportunities combining a high-performance FIB-SEM with femtosecond laser processing in an integrated second chamber","authors":"Ben Tordoff, Cheryl Hartfield, Andrew J. Holwell, Stephan Hiller, Marcus Kaestner, Stephen Kelly, Jaehan Lee, Sascha Müller, Fabian Perez-Willard, Tobias Volkenandt, Robin White, Thomas Rodgers","doi":"10.1186/s42649-020-00044-5","DOIUrl":"https://doi.org/10.1186/s42649-020-00044-5","url":null,"abstract":"The development of the femtosecond laser (fs laser) with its ability to provide extremely rapid athermal ablation of materials has initiated a renaissance in materials science. Sample milling rates for the fs laser are orders of magnitude greater than that of traditional focused ion beam (FIB) sources currently used. In combination with minimal surface post-processing requirements, this technology is proving to be a game changer for materials research. The development of a femtosecond laser attached to a focused ion beam scanning electron microscope (LaserFIB) enables numerous new capabilities, including access to deeply buried structures as well as the production of extremely large trenches, cross sections, pillars and TEM H-bars, all while preserving microstructure and avoiding or reducing FIB polishing. Several high impact applications are now possible due to this technology in the fields of crystallography, electronics, mechanical engineering, battery research and materials sample preparation. This review article summarizes the current opportunities for this new technology focusing on the materials science megatrends of engineering materials, energy materials and electronics.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00044-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5022535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Noise2Atom: unsupervised denoising for scanning transmission electron microscopy images Noise2Atom:扫描透射电子显微镜图像的无监督去噪

Applied Microscopy Pub Date : 2020-10-20 DOI: 10.1186/s42649-020-00041-8

Feng Wang, Trond R. Henninen, Debora Keller, Rolf Erni

{"title":"Noise2Atom: unsupervised denoising for scanning transmission electron microscopy images","authors":"Feng Wang, Trond R. Henninen, Debora Keller, Rolf Erni","doi":"10.1186/s42649-020-00041-8","DOIUrl":"https://doi.org/10.1186/s42649-020-00041-8","url":null,"abstract":"We propose an effective deep learning model to denoise scanning transmission electron microscopy (STEM) image series, named Noise2Atom, to map images from a source domain (mathcal {S}) to a target domain (mathcal {C}), where (mathcal {S}) is for our noisy experimental dataset, and (mathcal {C}) is for the desired clear atomic images. Noise2Atom uses two external networks to apply additional constraints from the domain knowledge. This model requires no signal prior, no noise model estimation, and no paired training images. The only assumption is that the inputs are acquired with identical experimental configurations. To evaluate the restoration performance of our model, as it is impossible to obtain ground truth for our experimental dataset, we propose consecutive structural similarity (CSS) for image quality assessment, based on the fact that the structures remain much the same as the previous frame(s) within small scan intervals. We demonstrate the superiority of our model by providing evaluation in terms of CSS and visual quality on different experimental datasets.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00041-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4804463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Method of Ga removal from a specimen on a microelectromechanical system-based chip for in-situ transmission electron microscopy 用于原位透射电子显微镜的基于微机电系统芯片的样品Ga去除方法

Applied Microscopy Pub Date : 2020-10-14 DOI: 10.1186/s42649-020-00043-6

Yena Kwon, Byeong-Seon An, Yeon-Ju Shin, Cheol-Woong Yang

{"title":"Method of Ga removal from a specimen on a microelectromechanical system-based chip for in-situ transmission electron microscopy","authors":"Yena Kwon, Byeong-Seon An, Yeon-Ju Shin, Cheol-Woong Yang","doi":"10.1186/s42649-020-00043-6","DOIUrl":"https://doi.org/10.1186/s42649-020-00043-6","url":null,"abstract":"In-situ transmission electron microscopy (TEM) holders that employ a chip-type specimen stage have been widely utilized in recent years. The specimen on the microelectromechanical system (MEMS)-based chip is commonly prepared by focused ion beam (FIB) milling and ex-situ lift-out (EXLO). However, the FIB-milled thin-foil specimens are inevitably contaminated with Ga+ ions. When these specimens are heated for real time observation, the Ga+ ions influence the reaction or aggregate in the protection layer. An effective method of removing the Ga residue by Ar+ ion milling within FIB system was explored in this study. However, the Ga residue remained in the thin-foil specimen that was extracted by EXLO from the trench after the conduct of Ar+ ion milling. To address this drawback, the thin-foil specimen was attached to an FIB lift-out grid, subjected to Ar+ ion milling, and subsequently transferred to an MEMS-based chip by EXLO. The removal of the Ga residue was confirmed by energy dispersive spectroscopy.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00043-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4591742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Sublimable materials facilitate the TEM sample preparation of oil-soluble nanomaterials 可升华材料有利于油溶性纳米材料的TEM样品制备

Applied Microscopy Pub Date : 2020-09-29 DOI: 10.1186/s42649-020-00042-7

Yu-Hao Deng

引用次数: 1

Fine structure of the intercalated disc and cardiac junctions in the black widow spider Latrodectus mactans 黑寡妇蜘蛛间插盘和心脏连接处的精细结构

Applied Microscopy Pub Date : 2020-09-25 DOI: 10.1186/s42649-020-00040-9

Yan Sun, Seung-Min Lee, Bon-Jin Ku, Myung-Jin Moon

{"title":"Fine structure of the intercalated disc and cardiac junctions in the black widow spider Latrodectus mactans","authors":"Yan Sun, Seung-Min Lee, Bon-Jin Ku, Myung-Jin Moon","doi":"10.1186/s42649-020-00040-9","DOIUrl":"https://doi.org/10.1186/s42649-020-00040-9","url":null,"abstract":"Arthropods have an open circulatory system with a simple tubular heart, so it has been estimated that the contractile pumping structure of the cardiac muscle will be less efficient than that of vertebrates. Nevertheless, certain arthropods are known to have far superior properties and characteristics than vertebrates, so we investigated the fine structural features of intercalated discs and cardiac junctions of cardiac muscle cells in the black widow spider Latrodectus mactans. Characteristically, the spider cardiac muscle has typical striated features and represents a functional syncytium that supports multiple connections to adjacent cells by intercalated discs. Histologically, the boundary lamina of each sarcolemma connects to the basement membrane to form an elastic sheath, and the extracellular matrix allows the cells to be anchored to other tissues. Since the intercalated disc is also part of sarcolemma, it contains gap junctions for depolarization and desmosomes that keep the fibers together during cardiac muscle contraction. Furthermore, fascia adherens and macula adherens (desmosomes) were also identified as cell junctions in both sarcolemma and intercalated discs. To enable the coordinated heartbeat of the cardiac muscle, the muscle fibers have neuronal innervations by multiple axons from the motor ganglion.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00040-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5382339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Methods to evaluate the twin formation energy: comparative studies of the atomic simulations and in-situ TEM tensile tests 评价孪晶形成能的方法:原子模拟与原位TEM拉伸试验的对比研究

Applied Microscopy Pub Date : 2020-09-17 DOI: 10.1186/s42649-020-00039-2

Hong-Kyu Kim, Sung-Hoon Kim, Jae-Pyoung Ahn

引用次数: 1

Microscopic research on the olfactory organ of the Far Eastern brook lamprey Lethenteron reissneri (Pisces, Petromyzontidae) 远东七鳃鳗嗅觉器官的显微研究(双鱼座，七鳃鳗科)

Applied Microscopy Pub Date : 2020-09-15 DOI: 10.1186/s42649-020-00038-3

Hyun-Tae Kim, Jong-Young Park

{"title":"Microscopic research on the olfactory organ of the Far Eastern brook lamprey Lethenteron reissneri (Pisces, Petromyzontidae)","authors":"Hyun-Tae Kim, Jong-Young Park","doi":"10.1186/s42649-020-00038-3","DOIUrl":"https://doi.org/10.1186/s42649-020-00038-3","url":null,"abstract":"The olfactory anatomy and histology of Lethenteron reissneri were researched using a stereo microscope, a light microscope, and a scanning electron microscope. As in other lampreys, it shows same characters as follows: i) a single olfactory organ, ii) a single tubular nostril, iii) a single olfactory chamber with gourd-like form, iv) a nasal valve, v) a nasopharyngeal pouch, vi) a sensory epithelium (SE) of continuous distribution, vii) a supporting cells with numerous long cilia, viii) an accessory olfactory organ. However, the description of a pseudostratified columnar layer in the SE and Non SE is a first record, not reported in sea lamprey Petromyzon marinus. In particular, both 19 to 20 lamellae in number and olfactory receptor neuron’s quarter ciliary length of the knob diameter differ from those of P. marinus. From these results, it might be considered that the olfactory organ of L. reissneri shows well adaptive structure of a primitive fish to slow flowing water with gravel, pebbles, and sand and a hiding habit into sand bottom at daytime. The lamellar number and neuron’s ciliary length may be a meaningful taxonomic character for the class Petromyzonida.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00038-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4922322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1