Applied Microscopy最新文献_第7页

Ultrastructure of the fertilized egg envelope from Melanotaenia praecox, Melanotaeniidae, Teleostei 早黑带绦虫(Melanotaeniidae, Teleostei)受精卵包膜的超微结构

Applied Microscopy Pub Date : 2021-04-01 DOI: 10.1186/s42649-021-00052-z

Joon Hyung Sohn, Dong Heui Kim

{"title":"Ultrastructure of the fertilized egg envelope from Melanotaenia praecox, Melanotaeniidae, Teleostei","authors":"Joon Hyung Sohn, Dong Heui Kim","doi":"10.1186/s42649-021-00052-z","DOIUrl":"https://doi.org/10.1186/s42649-021-00052-z","url":null,"abstract":"We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of dwarf rainbowfish (Melanotaenia praecox) belong to Melanotaeniidae using light and electron microscopes. The fertilized eggs were spherical with adhesive filament, transparent, demersal, and had a narrow perivitelline space and small oil droplets. The size of fertilized egg was 1.02?±?0.18?mm (n?=?30), and there were two kinds of adhesive filament on the fertilized eggs. The long and thick (diameter 12.22?±?0.52?μm, n?=?20) adhesive filaments were only at the area of animal pole, and short and thin (diameter 1.99?±?0.23?μm, n?=?20) adhesive filaments were around the long filaments. A micropyle was conical shaped with adhesive filament and located near the animal pole of egg. The outer surface of fertilized egg was rough side. Also, the total thickness of the fertilized egg envelope was about 7.46?±?0.41?μm (n?=?20), the fertilized egg envelope consisted of two layers, an inner lamellae layer and an outer layer with high electron-density. And the inner layer was 8 layers. Collectively, these morphological characteristics and adhesive property of fertilized egg with adhesive filaments, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-021-00052-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4001513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Methods of measuring presynaptic function with fluorescence probes 荧光探针测定突触前功能的方法

Applied Microscopy Pub Date : 2021-03-17 DOI: 10.1186/s42649-021-00051-0

Yeseul Jang, Sung Rae Kim, Sung Hoon Lee

引用次数: 2

Atomically sculptured heart in oxide film using convergent electron beam 利用会聚电子束在氧化膜中原子雕刻心脏

Applied Microscopy Pub Date : 2021-01-11 DOI: 10.1186/s42649-020-00050-7

Gwangyeob Lee, Seung-Hyub Baek, Hye Jung Chang

引用次数: 1

Protective effects of Erythronium japonicum and Corylopsis coreana Uyeki extracts against 1,3-dichloro-2-propanol-induced hepatotoxicity in rats 丹参和山茱萸提取物对1,3-二氯-2-丙醇所致大鼠肝毒性的保护作用

Applied Microscopy Pub Date : 2020-12-02 DOI: 10.1186/s42649-020-00049-0

Seunghyun Kim, Hee-Ock Boo, Taeho Ahn, Chun-Sik Bae

{"title":"Protective effects of Erythronium japonicum and Corylopsis coreana Uyeki extracts against 1,3-dichloro-2-propanol-induced hepatotoxicity in rats","authors":"Seunghyun Kim, Hee-Ock Boo, Taeho Ahn, Chun-Sik Bae","doi":"10.1186/s42649-020-00049-0","DOIUrl":"https://doi.org/10.1186/s42649-020-00049-0","url":null,"abstract":"Erythronium japonicum (E. japonicum) and Corylopsis coreana Uyeki (C. coreana Uyeki, Korean winter hazel) have been shown to significantly decrease 1,3-dichloro-2-propanol (1,3-DCP)-induced generation of reactive oxygen species and CYP2E1 activity in HuH7, human hepatocytes. In this study, we expanded upon the previous study and investigated the effects of E. japonicum and C. coreana Uyeki extracts on 1,3-DCP-induced liver damage in rats. The pre-treatment of rats with these extracts alleviated a decrease in body weight and reduced 1,3-DCP-induced increase in catalytic activities of hepatic enzymes, such as aspartate aminotransferase and alanine aminotransferase, in the serum. Moreover, treatment with the extracts restored the 1,3-DCP-induced decreases in anti-oxidant enzyme activities, such as the activities of superoxide dismutase and catalase, in the rat liver. Histopathological studies also strongly supported the results of enzyme activities. These results suggest a possibility that the extracts of E. japonicum and C. coreana Uyeki can be a remedy for alleviating 1,3-DCP-induced liver damage in animals.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00049-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4075462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Mechanical removal of surface residues on graphene for TEM characterizations 机械去除石墨烯表面残留物的TEM表征

Applied Microscopy Pub Date : 2020-11-30 DOI: 10.1186/s42649-020-00048-1

Dong-Gyu Kim, Sol Lee, Kwanpyo Kim

引用次数: 5

Sequential conversion from line defects to atomic clusters in monolayer WS2 单层WS2中从线缺陷到原子簇的顺序转换

Applied Microscopy Pub Date : 2020-11-30 DOI: 10.1186/s42649-020-00047-2

Gyeong Hee Ryu, Ren-Jie Chan

引用次数: 1

Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images 利用高分辨率扫描电镜图像分析了干细胞在纳米柱周期性阵列上的行为

Applied Microscopy Pub Date : 2020-11-17 DOI: 10.1186/s42649-020-00046-3

Jihun Kang, Eun-Hye Kang, Young-Shik Yun, Seungmuk Ji, In-Sik Yun, Jong-Souk Yeo

引用次数: 0

Scanning acoustic microscopy for material evaluation 用于材料评价的扫描声学显微镜

Applied Microscopy Pub Date : 2020-11-05 DOI: 10.1186/s42649-020-00045-4

Hyunung Yu

{"title":"Scanning acoustic microscopy for material evaluation","authors":"Hyunung Yu","doi":"10.1186/s42649-020-00045-4","DOIUrl":"https://doi.org/10.1186/s42649-020-00045-4","url":null,"abstract":"Scanning acoustic microscopy (SAM) or Acoustic Micro Imaging (AMI) is a powerful, non-destructive technique that can detect hidden defects in elastic and biological samples as well as non-transparent hard materials. By monitoring the internal features of a sample in three-dimensional integration, this technique can efficiently find physical defects such as cracks, voids, and delamination with high sensitivity. In recent years, advanced techniques such as ultrasound impedance microscopy, ultrasound speed microscopy, and scanning acoustic gigahertz microscopy have been developed for applications in industries and in the medical field to provide additional information on the internal stress, viscoelastic, and anisotropic, or nonlinear properties. X-ray, magnetic resonance, and infrared techniques are the other competitive and widely used methods. However, they have their own advantages and limitations owing to their inherent properties such as different light sources and sensors.This paper provides an overview of the principle of SAM and presents a few results to demonstrate the applications of modern acoustic imaging technology. A variety of inspection modes, such as vertical, horizontal, and diagonal cross-sections have been presented by employing the focus pathway and image reconstruction algorithm. Images have been reconstructed from the reflected echoes resulting from the change in the acoustic impedance at the interface of the material layers or defects. The results described in this paper indicate that the novel acoustic technology can expand the scope of SAM as a versatile diagnostic tool requiring less time and having a high efficiency.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00045-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4226738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

The LaserFIB: new application opportunities combining a high-performance FIB-SEM with femtosecond laser processing in an integrated second chamber LaserFIB:在集成的第二腔室中，将高性能FIB-SEM与飞秒激光处理相结合，提供了新的应用机会

Applied Microscopy Pub Date : 2020-10-26 DOI: 10.1186/s42649-020-00044-5

Ben Tordoff, Cheryl Hartfield, Andrew J. Holwell, Stephan Hiller, Marcus Kaestner, Stephen Kelly, Jaehan Lee, Sascha Müller, Fabian Perez-Willard, Tobias Volkenandt, Robin White, Thomas Rodgers

{"title":"The LaserFIB: new application opportunities combining a high-performance FIB-SEM with femtosecond laser processing in an integrated second chamber","authors":"Ben Tordoff, Cheryl Hartfield, Andrew J. Holwell, Stephan Hiller, Marcus Kaestner, Stephen Kelly, Jaehan Lee, Sascha Müller, Fabian Perez-Willard, Tobias Volkenandt, Robin White, Thomas Rodgers","doi":"10.1186/s42649-020-00044-5","DOIUrl":"https://doi.org/10.1186/s42649-020-00044-5","url":null,"abstract":"The development of the femtosecond laser (fs laser) with its ability to provide extremely rapid athermal ablation of materials has initiated a renaissance in materials science. Sample milling rates for the fs laser are orders of magnitude greater than that of traditional focused ion beam (FIB) sources currently used. In combination with minimal surface post-processing requirements, this technology is proving to be a game changer for materials research. The development of a femtosecond laser attached to a focused ion beam scanning electron microscope (LaserFIB) enables numerous new capabilities, including access to deeply buried structures as well as the production of extremely large trenches, cross sections, pillars and TEM H-bars, all while preserving microstructure and avoiding or reducing FIB polishing. Several high impact applications are now possible due to this technology in the fields of crystallography, electronics, mechanical engineering, battery research and materials sample preparation. This review article summarizes the current opportunities for this new technology focusing on the materials science megatrends of engineering materials, energy materials and electronics.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00044-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"5022535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Noise2Atom: unsupervised denoising for scanning transmission electron microscopy images Noise2Atom:扫描透射电子显微镜图像的无监督去噪

Applied Microscopy Pub Date : 2020-10-20 DOI: 10.1186/s42649-020-00041-8

Feng Wang, Trond R. Henninen, Debora Keller, Rolf Erni

{"title":"Noise2Atom: unsupervised denoising for scanning transmission electron microscopy images","authors":"Feng Wang, Trond R. Henninen, Debora Keller, Rolf Erni","doi":"10.1186/s42649-020-00041-8","DOIUrl":"https://doi.org/10.1186/s42649-020-00041-8","url":null,"abstract":"We propose an effective deep learning model to denoise scanning transmission electron microscopy (STEM) image series, named Noise2Atom, to map images from a source domain (mathcal {S}) to a target domain (mathcal {C}), where (mathcal {S}) is for our noisy experimental dataset, and (mathcal {C}) is for the desired clear atomic images. Noise2Atom uses two external networks to apply additional constraints from the domain knowledge. This model requires no signal prior, no noise model estimation, and no paired training images. The only assumption is that the inputs are acquired with identical experimental configurations. To evaluate the restoration performance of our model, as it is impossible to obtain ground truth for our experimental dataset, we propose consecutive structural similarity (CSS) for image quality assessment, based on the fact that the structures remain much the same as the previous frame(s) within small scan intervals. We demonstrate the superiority of our model by providing evaluation in terms of CSS and visual quality on different experimental datasets.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-020-00041-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4804463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28