Applied Microscopy最新文献_第6页

Role of Cel5H protein surface amino acids in binding with clay minerals and measurements of its forces Cel5H 蛋白质表面氨基酸在与粘土矿物结合中的作用及其作用力测量

Applied Microscopy Pub Date : 2021-11-11 DOI: 10.1186/s42649-021-00066-7

Renukaradhya K. Math, Nagakumar Bharatham, Palaksha K. Javaregowda, Han Dae Yun

{"title":"Role of Cel5H protein surface amino acids in binding with clay minerals and measurements of its forces","authors":"Renukaradhya K. Math, Nagakumar Bharatham, Palaksha K. Javaregowda, Han Dae Yun","doi":"10.1186/s42649-021-00066-7","DOIUrl":"10.1186/s42649-021-00066-7","url":null,"abstract":"<div>Our previous study on the binding activity between Cel5H and clay minerals showed highest binding efficiency among other cellulase enzymes cloned. Here, based on previous studies, we hypothesized that the positive amino acids on the surface of Cel5H protein may play an important role in binding to clay surfaces. To examine this, protein sequences of Bacillus licheniformis Cel5H (BlCel5H) and Paenibacillus polymyxa Cel5A (PpCel5A) were analyzed and then selected amino acids were mutated. These mutated proteins were investigated for binding activity and force measurement via atomic force microscopy (AFM). A total of seven amino acids which are only present in BlCel5H but not in PpCel5A were selected for mutational studies and the positive residues which are present in both were omitted. Of the seven selected surface lysine residues, only three mutants K196A(M2), K54A(M3) and K157T(M4) showed 12%, 7% and 8% less clay mineral binding ability, respectively compared with wild-type. The probable reason why other mutants did not show altered binding efficiency might be due to relative location of amino acids on the protein surface. Meanwhile, measurement of adhesion forces on mica sheets showed a well-defined maximum at 69 ± 19 pN for wild-type, 58 ± 19 pN for M2, 53 ± 19 pN for M3, and 49 ± 19 pN for M4 proteins. Hence, our results demonstrated that relative location of surface amino acids of Cel5H protein especially positive charged amino acids are important in the process of clay mineral-protein binding interaction through electrostatic exchange of charges.</div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8586110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39611173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Some living eukaryotes during and after scanning electron microscopy 扫描电子显微镜观察期间和观察之后的一些真核生物

Applied Microscopy Pub Date : 2021-11-04 DOI: 10.1186/s42649-021-00065-8

Ki Woo Kim

引用次数: 0

Teliospore mucilage of Puccinia miscanthi revealed through the axial imaging of secondary electrons 通过二次电子轴向成像揭示丝核菌的端孢子粘液

Applied Microscopy Pub Date : 2021-10-22 DOI: 10.1186/s42649-021-00064-9

Ki Woo Kim

引用次数: 0

Melanin-based structural coloration of birds and its biomimetic applications 基于黑色素的鸟类结构着色及其生物仿生应用。

Applied Microscopy Pub Date : 2021-10-11 DOI: 10.1186/s42649-021-00063-w

Deok-Jin Jeon, Suejeong Paik, Seungmuk Ji, Jong-Souk Yeo

引用次数: 0

Cryo-EM as a powerful tool for drug discovery: recent structural based studies of SARS-CoV-2 Cryo-EM作为药物发现的有力工具:最近基于SARS-CoV-2结构的研究。

Applied Microscopy Pub Date : 2021-09-25 DOI: 10.1186/s42649-021-00062-x

Han-ul Kim, Hyun Suk Jung

{"title":"Cryo-EM as a powerful tool for drug discovery: recent structural based studies of SARS-CoV-2","authors":"Han-ul Kim, Hyun Suk Jung","doi":"10.1186/s42649-021-00062-x","DOIUrl":"10.1186/s42649-021-00062-x","url":null,"abstract":"<div>The novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has arisen as a global pandemic affecting the respiratory system showing acute respiratory distress syndrome (ARDS). However, there is no targeted therapeutic agent yet and due to the growing cases of infections and the rising death tolls, discovery of the possible drug is the need of the hour. In general, the study for discovering therapeutic agent for SARS-CoV-2 is largely focused on large-scale screening with fragment-based drug discovery (FBDD). With the recent advancement in cryo-electron microscopy (Cryo-EM), it has become one of the widely used tools in structural biology. It is effective in investigating the structure of numerous proteins in high-resolution and also had an intense influence on drug discovery, determining the binding reaction and regulation of known drugs as well as leading the design and development of new drug candidates. Here, we review the application of cryo-EM in a structure-based drug design (SBDD) and in silico screening of the recently acquired FBDD in SARS-CoV-2. Such insights will help deliver better understanding in the procurement of the effective remedial solution for this pandemic.</div>","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8464538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39448854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature 多光谱活体内显微镜用于同时对微血管进行亮场和荧光成像。

Applied Microscopy Pub Date : 2021-07-24 DOI: 10.1186/s42649-021-00059-6

Barry G. H. Janssen, Mohamadreza Najiminaini, Yan Min Zhang, Parsa Omidi, Jeffrey J. L. Carson

引用次数: 0

Capture silk scaffold production in the cribellar web spider 捕丝脚手架生产在网蜘蛛

Applied Microscopy Pub Date : 2021-07-13 DOI: 10.1186/s42649-021-00061-y

Yan Sun, Seung-Min Lee, Bon-Jin Ku, Eun-Ah Park, Myung-Jin Moon

{"title":"Capture silk scaffold production in the cribellar web spider","authors":"Yan Sun, Seung-Min Lee, Bon-Jin Ku, Eun-Ah Park, Myung-Jin Moon","doi":"10.1186/s42649-021-00061-y","DOIUrl":"https://doi.org/10.1186/s42649-021-00061-y","url":null,"abstract":"Spider capture silk is a natural scaffolding material that outperforms most synthetic materials in terms of its combination of strength and elasticity. Among the various kinds of silk threads, cribellar thread is the most primitive prey-capturing type of spider web material. We analyzed the functional organization of the sieve-like cribellum spigots and specialized calamistral comb bristles for capture thread production by the titanoecid spider Nurscia albofasciata. The outer cribellar surface is covered with thousands of tiny spigots, and the cribellar plate produces non-sticky threads composed of thousands of fine nanofibers. N. albofasciata cribellar spigots are typically about 10?μm long, and each spigot appears as a long individual shaft with a pagoda-like tiered tip. The five distinct segments comprising each spigot is a defining characteristic of this spider. This segmented and flexible structure not only allows for spigots to bend individually and join with adjacent spigots, but it also enables spigots to draw the silk fibrils from their cribella with rows of calamistral leg bristles to form cribellar prey-capture threads.","PeriodicalId":470,"journal":{"name":"Applied Microscopy","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/s42649-021-00061-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"4540311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sensitivity of quantitative symmetry measurement algorithms for convergent beam electron diffraction technique 会聚束电子衍射技术中定量对称测量算法的灵敏度

Applied Microscopy Pub Date : 2021-07-03 DOI: 10.1186/s42649-021-00060-z

Hyeongsub So, Ro Woon Lee, Sung Taek Hong, Kyou-Hyun Kim

引用次数: 2

A brief review of non-invasive brain imaging technologies and the near-infrared optical bioimaging 无创脑成像技术与近红外光学生物成像技术综述

Applied Microscopy Pub Date : 2021-06-25 DOI: 10.1186/s42649-021-00058-7

Beomsue Kim, Hongmin Kim, Songhui Kim, Young-ran Hwang

引用次数: 15

TEM sample preparation using micro-manipulator for in-situ MEMS experiment 利用微机械臂制备TEM样品进行原位MEMS实验

Applied Microscopy Pub Date : 2021-06-09 DOI: 10.1186/s42649-021-00057-8

Hyunjong Lee, Odongo Francis Ngome Okello, Gi-Yeop Kim, Kyung Song, Si-Young Choi

引用次数: 2