Clinical and Experimental Reproductive Medicine-CERM最新文献_第7页

Preferred strategy for euploid single embryo transfer in advanced maternal age: Fresh versus frozen. 高龄产妇优生单胚胎移植的首选策略：新鲜胚胎与冷冻胚胎

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06233

Fatma Ozdemir, Gokalp Oner, Semra Kahraman, Yucel Sahin, Hakan Yelke

{"title":"Preferred strategy for euploid single embryo transfer in advanced maternal age: Fresh versus frozen.","authors":"Fatma Ozdemir, Gokalp Oner, Semra Kahraman, Yucel Sahin, Hakan Yelke","doi":"10.5653/cerm.2023.06233","DOIUrl":"10.5653/cerm.2023.06233","url":null,"abstract":"Objective: The purpose of this study was to compare fresh and frozen-thawed euploid blastocyst transfer protocols following preimplantation genetic screening (PGS) in cases of advanced maternal age.Methods: A total of 330 patients were examined retrospectively. PGS was performed on the embryos of 146 patients for whom fresh transfers were chosen. In contrast, frozen-thawed euploid single embryo transfer (ET) was selected after PGS for 184 patients, and their embryos were vitrified. The percentage of euploid embryos and rates of implantation, pregnancy, and pregnancy continuity, as well as clinical and biochemical abortion rates, were compared.Results: The numbers of retrieved oocytes, metaphase II oocytes, and fertilized ova were greater in the frozen-thawed group. The percentages of euploid embryos were comparable between the fresh and frozen-thawed groups (32% vs. 34.8%, respectively). The rates of implantation (46.6%vs. 62.5%), pregnancy (50% vs. 66.8%), ongoing pregnancy (38.4% vs. 53.8%), and live birth percentage (37.0% vs. 53.8%) were significantly higher in the frozen-thawed group. However, no significant differences were found in the clinical and biochemical abortion rates.Conclusion: The use of frozen-thawed single euploid ET is associated with increased implantation and pregnancy rates compared to fresh single euploid ET with PGS.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"85-90"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139541690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detrimental impact of cell phone radiation on sperm DNA integrity. 手机辐射对精子 DNA 完整性的有害影响

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2024-01-24 DOI: 10.5653/cerm.2023.06121

Yeganeh Koohestanidehaghi, Mohammad Ali Khalili, Fatemeh Dehghanpour, Mohammad Seify

{"title":"Detrimental impact of cell phone radiation on sperm DNA integrity.","authors":"Yeganeh Koohestanidehaghi, Mohammad Ali Khalili, Fatemeh Dehghanpour, Mohammad Seify","doi":"10.5653/cerm.2023.06121","DOIUrl":"10.5653/cerm.2023.06121","url":null,"abstract":"Radiofrequency electromagnetic radiation (RF-EMR) from various sources may impact health due to the generation of frequency bands. Broad pulses emitted within frequency bands can be absorbed by cells, influencing their function. Numerous laboratory studies have demonstrated that mobile phones-generally the most widely used devices-can have harmful effects on sex cells, such as sperm and oocytes, by producing RF-EMR. Moreover, some research has indicated that RF-EMR generated by mobile phones can influence sperm parameters, including motility, morphology, viability, and (most critically) DNA structure. Consequently, RF-EMR can disrupt both sperm function and fertilization. However, other studies have reported that exposure of spermatozoa to RF-EMR does not affect the functional parameters or genetic structure of sperm. These conflicting results likely stem from differences among studies in the duration and exposure distance, as well as the species of animal used. This report was undertaken to review the existing research discussing the effects of RF-EMR on the DNA integrity of mammalian spermatozoa.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"13-19"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139543323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Criteria for implementing artificial intelligence systems in reproductive medicine. 生殖医学人工智能系统实施标准。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06009

Enric Güell

引用次数: 0

Clinical outcomes of preimplantation genetic testing for aneuploidy in high-risk patients: a retrospective cohort study. 高风险患者植入前非整倍体基因检测的临床结果：一项回顾性队列研究。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2023-12-27 DOI: 10.5653/cerm.2023.06394

Jun Woo Kim, So Young Lee, Chang Young Hur, Jin Ho Lim, Choon Keun Park

{"title":"Clinical outcomes of preimplantation genetic testing for aneuploidy in high-risk patients: a retrospective cohort study.","authors":"Jun Woo Kim, So Young Lee, Chang Young Hur, Jin Ho Lim, Choon Keun Park","doi":"10.5653/cerm.2023.06394","DOIUrl":"10.5653/cerm.2023.06394","url":null,"abstract":"Objective: The purpose of this study was to evaluate the impact of preimplantation genetic testing for aneuploidy (PGT-A) on clinical outcomes among high-risk patients.Methods: This retrospective study involved 1,368 patients and the same number of cycles, including 520 cycles with PGT-A and 848 cycles without PGT-A. The study participants comprised women of advanced maternal age (AMA) and those affected by recurrent implantation failure (RIF), recurrent pregnancy loss (RPL), or severe male factor infertility (SMF).Results: PGT-A was associated with significant improvements in the implantation rate (IR) and the ongoing pregnancy rate/live birth rate (OPR/LBR) per embryo transfer cycle in the AMA (39.3% vs. 16.2% [p<0.001] and 42.0% vs. 21.8% [p<0.001], respectively), RIF (41.7% vs. 22.0% [p<0.001] and 47.0% vs. 28.6% [p<0.001], respectively), and RPL (45.6% vs. 19.5% [p<0.001] and 49.1% vs. 24.2% [p<0.001], respectively) groups, as well as the IR in the SMF group (43.3% vs. 26.5%, p=0.011). Additionally, PGT-A was associated with lower overall incidence rates of early pregnancy loss in the AMA (16.7% vs. 34.3%, p=0.001) and RPL (16.7% vs. 50.0%, p<0.001) groups. However, the OPR/LBR per total cycle across all PGT-A groups did not significantly exceed that for the non-PGT-A groups.Conclusion: PGT-A demonstrated beneficial effects in high-risk patients. However, our findings indicate that these benefits are more pronounced in carefully selected candidates than in the entire high-risk patient population.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"75-84"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of temperature and storage time on DNA integrity after freeze-drying sperm from individuals with normozoospermia. 温度和储存时间对正常精子症患者精子冷冻干燥后DNA完整性的影响。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI: 10.5653/cerm.2023.06093

Farzaneh Mohammadzadeh Kazorgah, Azam Govahi, Ali Dadseresht, Fatemeh Nejat Pish Kenari, Marziyeh Ajdary, Rana Mehdizadeh, Roya Derakhshan, Mehdi Mehdizadeh

{"title":"The effect of temperature and storage time on DNA integrity after freeze-drying sperm from individuals with normozoospermia.","authors":"Farzaneh Mohammadzadeh Kazorgah, Azam Govahi, Ali Dadseresht, Fatemeh Nejat Pish Kenari, Marziyeh Ajdary, Rana Mehdizadeh, Roya Derakhshan, Mehdi Mehdizadeh","doi":"10.5653/cerm.2023.06093","DOIUrl":"10.5653/cerm.2023.06093","url":null,"abstract":"Objective: This study evaluated the effects of temperature and storage time on the quality and DNA integrity of freeze-dried sperm from individuals with normozoospermia.Methods: Normal sperm samples from 15 men aged 24 to 40 years were studied. Each sample was divided into six groups: fresh, freezing (frozen in liquid nitrogen), freeze-dried then preserved at room temperature for 1 month (FD-1m-RT), freeze-dried then preserved at room temperature for 2 months (FD-2m-RT), freeze-dried then preserved at 4 °C for 1 month (FD-1m-4 °C), and freeze-dried then preserved at 4 °C for 2 months (FD-2m-4 °C). The morphology, progressive motility, vitality, and DNA integrity of the sperm were evaluated in all groups.Results: In all freeze-dried groups, sperm cells were immotile after rehydration. The freeze-dried groups also showed significantly less sperm vitality than the fresh and frozen groups. Significantly more morphological sperm abnormalities were found in the freeze-dried groups, but freeze-drying did not lead to a significantly higher DNA fragmentation index (DFI). The DFI was significantly higher in the FD-2m-RT group than in the other freeze-dried groups.Conclusion: The freeze-drying method preserved the integrity of sperm DNA. The temperature and duration of storage were also identified as factors that influenced the DFI. Accordingly, more research is needed on ways to improve sperm quality in the freeze-drying process.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"42-47"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138463434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predictors of twin pregnancy in in vitro fertilization with intracytoplasmic sperm injection cycles with day 3 double embryo transfer. 卵胞浆内单精子注射体外受精周期第 3 天双胚胎移植中双胎妊娠的预测因素。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2023-12-27 DOI: 10.5653/cerm.2023.06156

Duy Le Nguyen, Hieu Le-Trung Hoang, Vu Ngoc-Anh Ho, Toan Duong Pham, Nam Thanh Nguyen, Van Thi-Thu Tran, Tuong Manh Ho, Lan Ngoc Vuong

{"title":"Predictors of twin pregnancy in in vitro fertilization with intracytoplasmic sperm injection cycles with day 3 double embryo transfer.","authors":"Duy Le Nguyen, Hieu Le-Trung Hoang, Vu Ngoc-Anh Ho, Toan Duong Pham, Nam Thanh Nguyen, Van Thi-Thu Tran, Tuong Manh Ho, Lan Ngoc Vuong","doi":"10.5653/cerm.2023.06156","DOIUrl":"10.5653/cerm.2023.06156","url":null,"abstract":"Objective: The purpose of this study was to identify factors associated with twin pregnancy following day 3 double embryo transfer (DET).Methods: This retrospective cohort study incorporated data from 16,972 day 3 DET cycles. The participants were women aged between 18 and 45 years who underwent in vitro fertilization with intracytoplasmic sperm injection (IVF/ICSI) at My Duc Assisted Reproduction Technique Unit (IVFMD), My Duc Hospital, located in Ho Chi Minh City, Vietnam.Results: Of the 16,972 day 3 DET cycles investigated, 8,812 (51.9%) resulted in pregnancy. Of these, 6,108 cycles led to clinical pregnancy, with 1,543 (25.3% of clinical pregnancies) being twin pregnancies. Factors associated with twin pregnancy included age under 35 years (odds ratio [OR], 1.5; 95% confidence interval [CI], 1.32 to 1.71; p<0.001) and cycles involving the transfer of at least one grade I embryo. Relative to the transfer of two grade III embryos, the risk of twin pregnancy was significantly elevated following the transfer of two grade I embryos (OR, 1.40; 95% CI, 1.16 to 1.69; p<0.001) or a combination of one grade I and one grade II embryo (OR, 1.27; 95% CI, 1.05 to 1.55; p=0.001).Conclusion: By analyzing a large number of IVF/ICSI cycles, we identified several predictors of twin pregnancy. These findings can assist medical professionals in tailoring treatment strategies for couples with infertility.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"69-74"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia. 咖啡因可减轻慢性阴囊高热小鼠的生精障碍

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2024-02-29 DOI: 10.5653/cerm.2023.06142

Amir Raoofi, Omid Gholami, Hossein Mokhtari, Fatemeh Bagheri, Auob Rustamzadeh, Davood Nasiry, Alireza Ghaemi

{"title":"Caffeine attenuates spermatogenic disorders in mice with induced chronic scrotal hyperthermia.","authors":"Amir Raoofi, Omid Gholami, Hossein Mokhtari, Fatemeh Bagheri, Auob Rustamzadeh, Davood Nasiry, Alireza Ghaemi","doi":"10.5653/cerm.2023.06142","DOIUrl":"10.5653/cerm.2023.06142","url":null,"abstract":"Objective: Chronic scrotal hyperthermia (SHT) can lead to serious disorders of the male reproductive system, with oxidative stress playing a key role in the onset of these dysfunctions. Thus, we evaluated the impact of caffeine, a potent antioxidant, on cellular and tissue disorders in mice with chronic SHT.Methods: In this experimental study, 56 adult male NMRI mice were allocated into seven equal groups. Apart from the non-treated control group, all were exposed to heat stress. Two groups, termed \"preventive\" and \"curative,\" were orally administered caffeine. The preventive mice began receiving caffeine immediately prior to heat exposure, while for the curative group, a caffeine regimen was initiated 15 consecutive days following cessation of heat exposure. Each treated group was subdivided based on pairing with a positive control (Pre/curative [Cur]+PC) or a vehicle (Pre/Cur+vehicle). Upon conclusion of the study, we assessed sperm characteristics, testosterone levels, stereological parameters, apoptosis, antioxidant and oxidant levels, and molecular markers.Results: Sperm parameters, testosterone levels, stereological parameters, biochemical factors (excluding malondialdehyde [MDA]), and c-kit gene expression were significantly elevated in the preventive and curative groups, especially the former, relative to the other groups. Conversely, expression levels of the heat shock protein 72 (HSP72) and nuclear factor kappa beta (NF-κβ) genes, MDA levels, and apoptotic cell density were markedly lower in both caffeine-treated groups relative to the other groups, with more pronounced differences observed in the preventive group.Conclusion: Overall, caffeine attenuated cellular and molecular abnormalities induced by heat stress in the testis, particularly in the mice treated under the preventive condition.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"51 1","pages":"28-41"},"PeriodicalIF":1.8,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of preimplantation mouse embryo development with optimized in vitro culture dish via stabilization of medium osmolarity. 优化体外培养皿通过稳定培养基渗透压促进小鼠胚胎着床前发育。

IF 1.8

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2023-12-01 Epub Date: 2023-11-21 DOI: 10.5653/cerm.2023.06436

Hyejin Yoon, Jongwoo Lee, Inyoung Kang, Kyoo Wan Choi, Jaewang Lee, Jin Hyun Jun

{"title":"Enhancement of preimplantation mouse embryo development with optimized in vitro culture dish via stabilization of medium osmolarity.","authors":"Hyejin Yoon, Jongwoo Lee, Inyoung Kang, Kyoo Wan Choi, Jaewang Lee, Jin Hyun Jun","doi":"10.5653/cerm.2023.06436","DOIUrl":"10.5653/cerm.2023.06436","url":null,"abstract":"Objective: We evaluated the efficacy of the newly developed optimized in vitro culture (OIVC) dish for cultivating preimplantation mouse embryos. This dish minimizes the need for mineral oil and incorporates microwells, providing a stable culture environment and enabling independent monitoring of individual embryos.Methods: Mouse pronuclear (PN) zygotes and two-cell-stage embryos were collected at 18 and 46 hours after human chorionic gonadotropin injection, respectively. These were cultured for 120 hours using potassium simplex optimized medium (KSOM) to reach the blastocyst stage. The embryos were randomly allocated into three groups, each cultured in one of three dishes: a 60-mm culture dish, a microdrop dish, and an OIVC dish that we developed.Results: The OIVC dish effectively maintained the osmolarity of the KSOM culture medium over a 5-day period using only 2 mL of mineral oil. This contrasts with the significant osmolarity increase observed in the 60-mm culture dish. Additionally, the OIVC dish exhibited higher blastulation rates from two-cell embryos (100%) relative to the other dish types. Moreover, blastocysts derived from both PN zygotes and two-cell embryos in the OIVC dish group demonstrated significantly elevated mean cell numbers.Conclusion: Use of the OIVC dish markedly increased the number of cells in blastocysts derived from the in vitro culture of preimplantation mouse embryos. The capacity of this dish to maintain medium osmolarity with minimal mineral oil usage represents a breakthrough that may advance embryo culture techniques for various mammals, including human in vitro fertilization and embryo transfer programs.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":"50 4","pages":"244-252"},"PeriodicalIF":1.8,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10711242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138300298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of a short abstinence period on sperm quality in oligozoospermic men 短时间禁欲对少精子男性精子质量的影响

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2023-11-09 DOI: 10.5653/cerm.2023.06100

Nattaporn Poopaibool, Amornrat Tangprasittipap, Sukanya Chumchuen, Chonthicha Satirapod, Artitaya Singwongsa

{"title":"Effects of a short abstinence period on sperm quality in oligozoospermic men","authors":"Nattaporn Poopaibool, Amornrat Tangprasittipap, Sukanya Chumchuen, Chonthicha Satirapod, Artitaya Singwongsa","doi":"10.5653/cerm.2023.06100","DOIUrl":"https://doi.org/10.5653/cerm.2023.06100","url":null,"abstract":"Objective: The aim of this study was to compare semen parameters and sperm DNA fragmentation (SDF) and explore the relationship between semen parameters and SDF between 2 and 7 days of abstinence and a short abstinence period (within 4 hours) in oligozoospermic infertile patients.Methods: Two semen samples were collected from infertile oligozoospermic men (n=34) after an abstinence period of 2 to 7 days and within 4 hours, respectively. Sperm parameters were compared between the two abstinence duration groups, including semen volume, sperm concentration, total sperm count, sperm motility, total motile sperm count (TMSC), morphology, and SDF.Results: The semen volume, concentration, and total sperm count were significantly decreased after 4 hours of abstinence than after 2 to 7 days of abstinence, with median differences of 1.2 mL (p<0.001), 2×106/mL (p=0.011), and 9.6×106/ejaculation (p<0.001), respectively. TMSC was significantly lower after a short abstinence, with a median difference of 4.24×106/ejaculate (p<0.001). However, there were no significance differences in the percentage of motility, the SDF, and the percentage of sperm with normal morphology. Interestingly, volume, concentration, total sperm count, sperm motility, and SDF, but not TMSC, exhibited significant linear correlations between the two abstinence groups in univariate regression analysis, except for TMSC.Conclusion: In oligozoospermic men, the volume, concentration, and total sperm count were significantly lower after a short abstinence period, but without adverse effects on sperm motility and SDF.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" 36","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135244543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of berberine on ischemia-reperfusion injuries in an experimental model of ovarian torsion 小檗碱对卵巢扭转实验模型缺血再灌注损伤的影响

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2023-11-09 DOI: 10.5653/cerm.2023.06366

Filiz Yilmaz, Orkun Ilgen, Alper Mankan, Bayram Yilmaz, Sefa Kurt

{"title":"The effects of berberine on ischemia-reperfusion injuries in an experimental model of ovarian torsion","authors":"Filiz Yilmaz, Orkun Ilgen, Alper Mankan, Bayram Yilmaz, Sefa Kurt","doi":"10.5653/cerm.2023.06366","DOIUrl":"https://doi.org/10.5653/cerm.2023.06366","url":null,"abstract":"Objective: Ovarian torsion is a gynecological disorder that causes ischemia-reperfusion injuries in the ovary. Our study investigated berberine’s short- and long-term effects on ovarian ischemia-reperfusion injuries.Methods: This study included 28 Wistar albino female rats weighing 180 to 220 g, which were divided into four groups: sham (S), torsion/detorsion (T/D), torsion/ detorsion+single dose berberine (T/D+Bb), and torsion/detorsion+15 days berberine (T/D+15Bb). The torsion and detorsion model was applied in all non-sham groups. In the T/D+Bb group, a single dose of berberine was administered, while in the T/D+15Bb group, berberine was administered over a period of 15 days. After the rats were euthanized, their ovaries were excised. The left ovaries were used for histopathologic evaluation, which included ovarian injury scoring and follicle count, while the right ovaries were used for biochemical analyses (tissue transforming growth factor-β [TGF-β] and alpha-smooth muscle actin [α-SMA] levels).Results: The histopathologic evaluation scores for the ovaries were significantly lower in the T/D+B group (p<0.05) and the T/D+15B group (p<0.005) than in the T/D group. The follicle counts in the T/D group were lower than those in both the sham and treated groups (p<0.005). The TGF-β levels were significantly lower in the T/D+15B group (p<0.005), whereas the α-SMA levels did not show a significant difference.Conclusion: Both short- and long-term berberine use could potentially have therapeutic effects on ovarian torsion. Long-term berberine use exhibited anti-inflammatory effects by reducing TGF-β levels, thereby preventing ischemia-reperfusion injuries. Therefore, we suggest that long-term berberine use could be beneficial for ovarian torsion.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" 33","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135192387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0