The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY

Clinical and Experimental Reproductive Medicine-CERM Pub Date : 2024-03-01 Epub Date: 2023-12-01 DOI:10.5653/cerm.2023.06079

Nafiseh Tavakolpoor Saleh, Zohreh Hosseinzadeh, Narges Gholami Banadkuki, Maryam Salehi Novin, Sanaz Saljooghi Zaman, Tohid Moradi Gardeshi

{"title":"The protective effect of zinc oxide and selenium oxide nanoparticles on the functional parameters of rat sperm during vitrification.","authors":"Nafiseh Tavakolpoor Saleh, Zohreh Hosseinzadeh, Narges Gholami Banadkuki, Maryam Salehi Novin, Sanaz Saljooghi Zaman, Tohid Moradi Gardeshi","doi":"10.5653/cerm.2023.06079","DOIUrl":null,"url":null,"abstract":"Objective: While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels.Methods: Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 μg/mL) and ZnONPs (0.1, 1, 10 μg/mL).Results: Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 μg/mL SeONPs and 1 μg/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05).Conclusion: The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.","PeriodicalId":46409,"journal":{"name":"Clinical and Experimental Reproductive Medicine-CERM","volume":" ","pages":"20-27"},"PeriodicalIF":1.8000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10914496/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical and Experimental Reproductive Medicine-CERM","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5653/cerm.2023.06079","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/1 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: While sperm freezing (cryopreservation) is an effective method for preserving fertility, it can potentially harm the structure and function of sperm due to an increase in the production of reactive oxygen species. This study aimed to assess the impact of zinc oxide nanoparticles (ZnONPs) and selenium oxide nanoparticles (SeONPs) on various sperm functional parameters, including motility, plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), acrosome membrane integrity (ACi), and malondialdehyde (MDA) levels.

Methods: Semen samples were collected from 20 Albino Wistar rats. These samples were then divided into six groups: fresh, cryopreservation control, and groups supplemented with SeONPs (1, 2, 5 μg/mL) and ZnONPs (0.1, 1, 10 μg/mL).

Results: Statistical analysis revealed that all concentrations of SeONPs increased total motility and progressive reduction of MDA levels compared to the cryopreservation control group (p<0.05). However, supplementation with ZnONPs did not affect these parameters (p>0.05). Conversely, supplements of 1 and 2 μg/mL SeONPs and 1 μg/mL ZnONPs contributed to the improvement of PMI and ACi (p<0.05). Yet, no significant change was observed in MMP with any concentration of SeONPs and ZnONPs compared to the cryopreservation control group (p>0.05).

Conclusion: The findings suggest that optimal concentrations of SeONPs may enhance sperm parameters during the freezing process.

查看原文本刊更多论文

氧化锌和氧化硒纳米颗粒对大鼠精子玻璃化过程中功能参数的保护作用。

目的:虽然精子冷冻(冷冻保存)是一种有效的保存生育能力的方法，但由于活性氧的产生增加，它可能会损害精子的结构和功能。本研究旨在评估氧化锌纳米颗粒(ZnONPs)和氧化硒纳米颗粒(SeONPs)对精子各种功能参数的影响，包括运动性、质膜完整性(PMI)、线粒体膜电位(MMP)、顶体膜完整性(ACi)和丙二醛(MDA)水平。方法:采集20只白化Wistar大鼠精液标本。将样品分为新鲜组、冷冻对照组和添加SeONPs组(1、2、5 μg/mL)和ZnONPs组(0.1、1、10 μg/mL)。结果:与低温保存对照组相比，各浓度SeONPs均使大鼠总运动性升高，MDA水平逐渐降低(p0.05)。相反，添加1、2 μg/mL SeONPs和1 μg/mL ZnONPs对PMI和ACi有改善作用(p0.05)。结论:最佳浓度的SeONPs可提高精子在冷冻过程中的各项参数。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical and Experimental Reproductive Medicine-CERM Multiple-

CiteScore

3.30

自引率

0.00%

发文量