Clinical Plasma Medicine最新文献

{"title":"Publishers Note","authors":"","doi":"10.1016/S2212-8166(19)30006-X","DOIUrl":"https://doi.org/10.1016/S2212-8166(19)30006-X","url":null,"abstract":"","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S2212-8166(19)30006-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137000362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety evaluation of the plasma on ocular surface tissue: An animal study and histopathological findings","authors":"Farhad Nejat,&nbsp;Nazanin-Sadat Nabavi,&nbsp;Mohammad-Amin Nejat,&nbsp;Hossein Aghamollaei,&nbsp;Khosrow Jadidi","doi":"10.1016/j.cpme.2019.100084","DOIUrl":"10.1016/j.cpme.2019.100084","url":null,"abstract":"<div><h3>Purpose</h3><p>The plasma soft surgery is as an alternative to invasive surgical cosmetic procedures that can reduce the recovery time and possible complications after surgery. Due to the sensitivity of ocular surface tissue and the potential of the plasma, it can be evaluated to treat some ocular surface disorders. Accordingly, we evaluated the safety of the cold plasma on the ocular surface tissue in three areas located in the cornea, limbus, and conjunctiva.</p></div><div><h3>Methods</h3><p>Nine adult male New Zealand albino rabbits which divided into three groups were used for experiments. The left eye of each rabbit was chosen for test and the right eye was as the control. Experiments were performed on three ocular surface areas under the influence of the plasma. For this purpose, the plasma was created by Plexr device in continues mode and low power level (white handpiece; 0.7 W) was utilized at 0.7 s intervals using a 22-gauge needle. For evaluation of plasma safety, ocular surface integrity and histopathological changes were assessed after 24 h (A), one week (B), and one month (C) exposure to plasma.</p></div><div><h3>Results</h3><p><span><span>According to the external and slit-lamp examinations, after 24 h (A) only epithelial defect was visible while ocular surface in other groups (B &amp; C) was healthy had completely recovered. </span>OCT imaging and histologic examination also confirmed these results. After 24 h conjunctival and corneal sections showed a localized area of epithelial loss together with </span>infiltration<span><span> of inflammatory cells<span> in the subepithelial stroma<span>, but during the one-month period, recovery was evident. Generally, after the first week, the loss of integrity of corneal epithelium has not been completely improved yet, while it was recovered nearly to be normal in group C. It was noteworthy that in all groups the loss of </span></span></span>endothelium cells was not apparent which confirming the lack of damage to the deep layers of the eye.</span></p></div><div><h3>Conclusion</h3><p>According to the plasma safety results, it seems that plasma soft surgery has the potential to be used as an alternative method for treatment of some of the ocular surface disorders without needing to conventional surgical procedures.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2019.100084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74925854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"When plasma jet is effective for chronic wound bacteria inactivation, is it also effective for wound healing?","authors":"Sri Darmawati ,&nbsp;Afiana Rohmani ,&nbsp;Laela Hayu Nurani ,&nbsp;Muhammad Evy Prastiyanto ,&nbsp;Sri Sinto Dewi ,&nbsp;Nia Salsabila ,&nbsp;Eka Sakti Wahyuningtyas ,&nbsp;Fri Murdiya ,&nbsp;Isabella Meliawati Sikumbang ,&nbsp;Ratnasari Nur Rohmah ,&nbsp;Yun Arifatul Fatimah ,&nbsp;Andi Widiyanto ,&nbsp;Tatsuo Ishijima ,&nbsp;Junko Sugama ,&nbsp;Toshio Nakatani ,&nbsp;N Nasruddin","doi":"10.1016/j.cpme.2019.100085","DOIUrl":"10.1016/j.cpme.2019.100085","url":null,"abstract":"<div><h3>Purpose</h3><p>This investigation aimed to compare the effectiveness of two styles of plasma jet treatment (i.e., contact and non-contact styles) for two biological materials, namely, wound related bacteria and acute wounds.</p></div><div><h3>Method</h3><p>An atmospheric plasma jet operated at a frequency of 18.32 kHz and high AC voltage with a peak-to-peak voltage of 9.58 kV and a current of 55.2 mA was applied. Argon gas was used as the carries gas of plasma jet generation and was fixed at a flow rate of 1 standard liters per minute (slm).Two biological materials (i.e., wound related bacteria and acute wound) were applied as experimental objects. The sample groups were based on the two styles of plasma jet treatment: contact and non-contact styles. Microbial inhibition zone calculation and macroscopic and histological observations were also performed.</p></div><div><h3>Results</h3><p>This investigation emphasized that the contact and non-contact styles of plasma jet treatment had significantly different effects for wounds and wound-related chronic bacteria. On the one hand, the contact style was visually attractive and more effective for inactivate bacteria. On the other hand, it caused negative effects, such as damaging normal tissue, significantly impeding wound healing and impeding the growing of new epithelial tissue. The non-contact style, however, was less effective at inactivating bacteria; however, it could accelerate wound healing.</p></div><div><h3>Conclusion</h3><p>In the context of wound healing, the non-contact style of plasma jet treatment may be better than the contact style of plasma jet treatment.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2019.100085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81448836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold physical plasma-induced oxidation of cysteine yields reactive sulfur species (RSS)","authors":"Giuliana Bruno ,&nbsp;Thea Heusler ,&nbsp;Jan-Wilm Lackmann ,&nbsp;Thomas von Woedtke ,&nbsp;Klaus-Dieter Weltmann ,&nbsp;Kristian Wende","doi":"10.1016/j.cpme.2019.100083","DOIUrl":"10.1016/j.cpme.2019.100083","url":null,"abstract":"<div><h3>Purpose</h3><p>Studying plasma liquid chemistry can reveal insights into their biomedical effects, i.e. to understand the direct and indirect processes triggered by the treatment in a model or clinical application. Due to the reactivity of the sulfur atom, thiols are potential targets for plasma- derived reactive species. Being crucial for protein function and redox signaling pathways, their controllable modification would allow expanding the application range. Additionally, models to control and standardize CAP sources are desired tools for plasma source design.</p></div><div><h3>Methods</h3><p>Cysteine, a ubiquitous amino acid, was used as a tracer compound to scavenge the reactive species produced by an argon plasma jet (kINPen). The resulting product pattern was identified via high-resolution mass spectrometry. The Ellman´s assay was used to screen CAP derived thiol consumption, and long-lived species deposition (hydrogen peroxide, nitrite, nitrate) was monitored in relation to the presence of cysteine.</p></div><div><h3>Results</h3><p>The intensity of cysteine oxidation increased with treatment time and availability of oxygen in the feed gas. A range of products from cysteine was identified, in part indicative for certain treatment conditions. Several non-stable products occur transiently during the plasma treatment. Bioactive reactive sulfur species (RSS) have been found for mild treatment conditions, such as cysteine sulfoxides and cysteine-<em>S</em>-sulfonate. Considering the number of cysteine molecules in the boundary layer and the achieved oxidation state, short-lived species dominate in cysteine conversion. In addition, a boundary layer depletion of the tracer was observed.</p></div><div><h3>Conclusion</h3><p>Translating these data into the in-vivo application, strong direct oxidation of protein thiol groups with subsequent changes in protein biochemistry must be considered. Plasma-derived RSS may in part contribute to the observed biomedical effects of CAP. Care must be taken to control the discharge parameter tightly as chemical dynamics at or in the liquid are subject to change easily.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2019.100083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80674715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can the effect of cold physical plasma-derived oxidants be transported via thiol group oxidation?","authors":"Thea Heusler ,&nbsp;Giuliana Bruno ,&nbsp;Sander Bekeschus ,&nbsp;Jan-Wilm Lackmann ,&nbsp;Thomas von Woedtke ,&nbsp;Kristian Wende","doi":"10.1016/j.cpme.2019.100086","DOIUrl":"10.1016/j.cpme.2019.100086","url":null,"abstract":"<div><h3>Purpose</h3><p>Intra- and intercellular redox-signaling processes where found responsible in various physiological and pathological processes with cellular thiol groups as important signal transducers. Using cold atmospheric plasma (CAP), a similar oxidation pattern of thiol groups can be achieved. Hence, it must be clarified which role extracellular thiol groups play in mediating CAP effects and whether or not the effects of short-lived reactive species can be preserved in a molecule like cysteine.</p></div><div><h3>Methods</h3><p>Physiological buffer solutions containing the amino acid cysteine were treated by an MHz argon plasma jet with molecular gas admixtures (kINPen) and transferred to cultured human keratinocytes. Cell proliferation, migratory activity, and metabolism were investigated. High-resolution mass spectrometry was used to estimate the impact of plasma generated species on thiol groups.</p></div><div><h3>Results</h3><p>While treated physiologic cysteine concentrations showed no impact on cell behavior, artificially high concentrations decreased proliferation, migration and lactate secretion. GSH levels inside cells were stabilized.</p></div><div><h3>Conclusion</h3><p>Extracellular thiol groups scavenge plasma-generated species and form a multitude of covalent modifications. Unexpectedly, human keratinocytes show only small functional consequences for treated physiologic cysteine concentrations. Results for high concentrated cysteine solutions indicate an improved cytostatic/cytotoxic impact by plasma treatment suggesting a potential application as a “preserving agent” of the chemical energy of plasma-derived species.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2019.100086","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88800043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of a Cold Atmospheric Pressure Plasma jet device for indirect treatment of Squamous Cell Carcinoma","authors":"S. Pereira,&nbsp;E. Pinto,&nbsp;P.A. Ribeiro,&nbsp;S. Sério","doi":"10.1016/j.cpme.2018.09.001","DOIUrl":"10.1016/j.cpme.2018.09.001","url":null,"abstract":"<div><h3>Purpose</h3><p>The possibility of obtaining plasmas at low temperatures and atmospheric conditions, the Cold Atmospheric Plasmas (CAPs), has triggered several studies on its possible medical applications. The use of CAPs devices applied to oncological treatments is still in a pre-clinical stage, but early investigations have shown promising results, both <em>in vitro</em> and <em>in vivo</em><span>. In this work, is reported the study of a custom made Cold Atmospheric Pressure Plasma jet device for indirect treatment of Squamous Cell Carcinoma.</span></p></div><div><h3>Methods</h3><p>The plasma plume produced by the jet device was characterized by optical emission spectroscopy and the vulnerability of two different cell lines, SCC-15 (Squamous Cell Carcinoma) and HGF-1 (non-cancer cell line) to different working parameters factors such as the volume of medium used on indirect plasma treatments, the number of cells used in the biological assays and the volume of treated medium in contact with the cultured cells were systematically tested and studied.</p></div><div><h3>Results</h3><p>The effectiveness of CAPs’ treatments is time, volume and cell dependent. Cancer cells, specifically SCC-15 cells have shown to be more sensitive to indirect plasma treatments than the non-cancer cell line used in the present work.</p></div><div><h3>Conclusions</h3><p>According to the obtained results, this study showed that thein <em>vitro</em> anti-cancer capacity of the developed jet plasma device will be higher when small volumes of medium are used together with moderate plasma treatment times, keeping the non-cancerous cells almost unaffected.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2018.09.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89391248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold atmospheric plasma (CAP) differently affects migration and differentiation of keratinocytes via hydrogen peroxide and nitric oxide-related products","authors":"Julian Balzer ,&nbsp;Erhan Demir ,&nbsp;Friederike Kogelheide ,&nbsp;Paul C. Fuchs ,&nbsp;Katharina Stapelmann ,&nbsp;Christian Opländer","doi":"10.1016/j.cpme.2018.11.001","DOIUrl":"10.1016/j.cpme.2018.11.001","url":null,"abstract":"<div><h3>Background</h3><p><span><span>A promising approach to treat infected chronic wounds is the treatment<span> with “cold” atmospheric plasma (CAP) that has a broad antibacterial spectrum and can enhance microcirculation. </span></span>Dielectric barrier discharge (DBD) devices generate CAP containing reactive species, leading to acidification and the accumulation of hydrogen peroxide (H</span>₂O₂), nitrite and nitrate within the treated tissue/liquids.</p></div><div><h3>Objective</h3><p>Since CAP produced species may affect wound healing and cell behavior, we investigated the possible DBD/CAP-induced effects on human keratinocytes.</p></div><div><h3>Methods</h3><p>Primary keratinocytes were treated by a DBD device (13.5 kV, 300 Hz; 0–300 s). DBD-induced changes (pH; nitrite, nitrate; H₂O₂) in treated media were evaluated. As control and to investigate the impact of the CAP-produced species, equivalents amounts of H₂O₂<span>, HCL, nitrite and nitrate as obtained by CAP treatments (0, 60, 300 s) were added separately or combined to keratinocytes. Cell viability and proliferation were determined by live cell imaging<span> and a resazurin-based assay. Gap closure rates were assessed by migration assays. Differentiation/proliferation states were determined by qRT-PCR analysis of KI67 and involucrin.</span></span></p></div><div><h3>Results</h3><p><span>We found that even longer CAP-treatment times (300 s) did not reduce cell viability. However, migration/proliferation was affected by longer treatments resulting in a delay of gap closure in migration assays. The mRNA expression of involucrin and KI67 showed a pro-differentiation effect induced by longer CAP treatment. Similar effects could be induced by adding H</span>₂O₂<span> in amounts found after a 300 s CAP treatment. The effects were reversed by catalase. Shorter CAP treatment (60 s) did not reveal pro-differentiation effects, but significantly accelerated gap closure. Lower H</span>₂O₂ concentrations, equivalent to a 60 s CAP treatment, induced also upregulation of involucrin, which in turn could be diminished by low concentrations of nitrite/nitrate, indicating a potential mediation of H₂O₂<span>-induced effects by parallel CAP-induced accumulation of these nitric oxide derivatives.</span></p></div><div><h3>Conclusion</h3><p><span>CAP treatment theoretically could kill several birds with one stone—overcome bacterial contamination, improve microcirculation and additionally compensate missing H</span>₂O₂ and nitric oxide— facilitating wound healing. However, clinical CAP treatment must be well balanced to avoid possible unwanted side effects, such as a delayed healing process and tissue damage.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2018.11.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88816043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potentiating anti-tumor immunity with physical plasma","authors":"Sander Bekeschus ,&nbsp;Ramona Clemen ,&nbsp;Hans-Robert Metelmann","doi":"10.1016/j.cpme.2018.10.001","DOIUrl":"10.1016/j.cpme.2018.10.001","url":null,"abstract":"<div><p>The age of checkpoint blockage emphasizes the importance of adaptive antitumor immune responses. This arm of immune defense is key in recognizing molecules via specific receptors to distinguish between self and foreign or mutated structures. Antigen-specific T-cells identify non-self epitopes, tumor-associated antigens, or neoepitopes on tumors to carry out attacks on malignant cells. Although tumor cells are immunogenic by nature, they have developed strategies to evade an immune response that would otherwise facilitate their clearance. Several steps in antitumor immunity utilize the toxic and signaling properties of reactive oxygen and nitrogen species (ROS/RNS). Cold physical plasmas are potent generators of such ROS/RNS and are demonstrated to have profound antitumor activity <em>in vitro</em> and <em>in vivo</em>. Here we discuss recent evidence and concepts on how plasmas may boost immunity against pathological cells. Specifically, plasma treatment may enhance the immunogenicity of tumor cells by induction of the immunogenic cancer cell death (ICD) and redox regulation of the antigen-presenting machinery. These aspects provide a rationale for localized plasma-based onco-therapies enhancing systemic antitumor immunity, which eventually may target distant tumor metastasis in cancer patients in a T-cell dependent fashion.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2018.10.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85839434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface treatment with non-thermal humid argon plasma as a treatment for allergic contact dermatitis in a mouse model","authors":"Xiong Qing ,&nbsp;Wang Xia ,&nbsp;Yin Rui ,&nbsp;Xiong Lin ,&nbsp;Chen Qiang ,&nbsp;Zheng Meng-Xue ,&nbsp;Xu Le ,&nbsp;Huang Qing-Hua ,&nbsp;Michael R Hamblin","doi":"10.1016/j.cpme.2018.09.002","DOIUrl":"10.1016/j.cpme.2018.09.002","url":null,"abstract":"<div><h3>Purpose</h3><p><span><span>Cold plasma generated at atmospheric pressure has attracted intense interest in biomedical applications. However, studies of cold plasma in dermatology, and as a possible therapy for non-infectious skin diseases, including burn and wound healing, </span>psoriasis<span>, and allergic contact dermatitis (ACD) </span></span><em>etc.</em><span>, are still quite scarce. The present study reports the treatment of ACD </span><em>in vivo</em>, using a non-thermal humid plasma source in a mouse model and shows the latter could be a potential alternative approach for therapy of ACD and other inflammatory skin diseases.</p></div><div><h3>Methods</h3><p><span>The model of ACD on the mouse back was induced by a solution of DNFB and treated by argon plasma containing small amounts of either N</span>₂, O₂, or H₂O. The developments of ACD regions were photographed and histopathological analysis by H&amp;E-staining was performed.</p></div><div><h3>Results</h3><p>The best effect was obtained using humid plasma (H₂<span>O addition), where the ACD symptoms decreased after one or two 1-min plasma treatments. Even for severe ACD with ulcers and crust formation, the humid plasma-treated mice recovered faster than the control group.</span></p></div><div><h3>Conclusions</h3><p><span>The therapeutic ability of the humid argon plasma discharge was proposed to be induced by reactive oxygen species (H</span>_xO_y<span>) transported from the discharge zone, which are adhesive and accumulate on the skin surface, penetrating the subcutis to eliminate inflammation. However, in treatments using plasma with addition of oxygen or nitrogen (without water) the active gaseous species are blocked due to poor adhesion to and penetration into the dry ACD skin, with correspondingly poor treatment effects. The enhanced </span><em>in vivo</em> healing in ACD mice indicate the non-thermal humid plasma could be a potential alternative approach for therapy of ACD and other inflammatory skin diseases.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2018.09.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37418402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on Manuka and Indonesian honeys to support the application of plasma jet during proliferative phase on wound healing","authors":"Eka Sakti Wahyuningtyas ,&nbsp;Arya Iswara ,&nbsp;Yunita Sari ,&nbsp;Sodiq Kamal ,&nbsp;Budi Santosa ,&nbsp;Tatsuo Ishijima ,&nbsp;Toshio Nakatani ,&nbsp;Indri Kartika Putri ,&nbsp;N Nasruddin","doi":"10.1016/j.cpme.2018.08.001","DOIUrl":"10.1016/j.cpme.2018.08.001","url":null,"abstract":"<div><h3>Purpose</h3><p>A comparative investigation was conducted to determine the effectiveness of Manuka and Indonesian honeys to support the application of plasma jet treatment during the proliferative phase of cutaneous wound healing in 8-week-old, BALB/c male mice.</p></div><div><h3>Methods</h3><p>The effect of honey containing different concentrations to reactive oxygen and nitrogen species (RONS) produced by plasma jet in liquid medium using H₂O₂<span> as the indicator was conducted using chemical-enzymatic method. Plasma jet treatment was applied perpendicularly to wounds through holes punched in multiple micro-well dressings (MMD) using direct contact. Mice were divided into 4 groups: Hydrocolloid dressing<span> alone (Control group or C), plasma application followed by hydrocolloid dressing (PH), plasma application followed by treatment with Indonesian honey (PI), and plasma application followed by treatment with Manuka honey (PM). Two full-thickness acute wounds were created on both sides of the mouse dorsum using a disposable biopsy punch. The wounds of the control group were covered with a hydrocolloid dressing (HD), whereas wounds in the other groups were covered with a HD from days 0 to 3, treated with plasma followed by 0.1 mL of the relevant honey or HD from days 4 to 7 post-wounding, and then were covered with a HD from days 8 to 14.</span></span></p></div><div><h3>Results</h3><p>On day 7the wound area in the PI and PM groups was smaller than in the control group. On days 12, 13, and 14, however, the wound area in PI-treated mice was significantly larger than in PM mice.</p></div><div><h3>Conclusion</h3><p>Manuka honey may better support plasma jet treatment than Indonesian honey on account of its chemical characteristics.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2018.08.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87833287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}