Clinical Plasma Medicine最新文献

{"title":"Microarry Analysis Identifies Activation Of Multiple Signalling Pathways In Primary Prostate Epithelial Cells By Low Temperature Plasma","authors":"John Packer ,&nbsp;Adam Hirst ,&nbsp;Fiona Frame ,&nbsp;Norman Maitland ,&nbsp;Deborah O’Connell","doi":"10.1016/j.cpme.2017.12.033","DOIUrl":"10.1016/j.cpme.2017.12.033","url":null,"abstract":"<div><p>We have previously reported the killing effects of Low Temperature Plasma (LTP) on primary prostate epithelial cells, of both normal and cancerous origin, via necrosis <strong>[1]</strong> following initial DNA damage. However it was evident that a population of cells was resistant to LTP treatment. To discern possible survival mechanisms in treated cells; the gene expression of 6 samples (4 cancers and 2 matched normal) was analysed 2 hours post-treatment, on a transcriptome wide level, by microarray. Initial analysis found alteration in the expression of 646 transcripts with significant activation of Notch, NF-kB, Nrf2 and AP-1 signalling in our primary cells. Pathway activation was subsequently validated by qRT-PCR in four different patient-derived prostate cultures to give a robust LTP-induced expression signature.</p><p>Upstream effector activation was then confirmed by immunofluorescence and Western blot. We found as other groups have; <strong>[2, 3]</strong> accumulation of Nrf2 in response to LTP, alongside AP-1 activation <strong>[4, 5]</strong> – through phosphorylation of both JNK and Jun. The Notch signalling pathway is typically described in relation to stem cell maintenance and we observed LTP induced Notch activation through release of its intracellular domain (NICD) that then acts as a nuclear transcription factor.</p><p>Tumours are innately heterogeneous and contain multiple cell populations, both intrinsic and recruited. We have established that our cultures contain three readily identifiable epithelial cell pools that can be separated to permit further analysis.<strong>[6]</strong> Preliminary investigation has found that the progenitor epithelial cells respond in greater measure than their more differentiated progeny, suggesting that they are more resistant to plasma. Similar observations of prostate stem cell resistance have been made with radiotherapy.<strong>[7]</strong> However, if these stem-like cells can be targeted – LTP may have a greater killing effect if utilized in prostate cancer treatments.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (151KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure – Pathway activation in primary prostate cancer epithelial culture; Nrf2 accumulation, AP-1 activation by JNK and Jun phosphorylation and Notch cleavage, with release of NICD by LTP. U – Untreated, T- Treated. Samples taken 0.5 and 2 hours following treatment with 3 minutes LTP.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77077545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atmospheric Pressure Multijet Plasma Sources For Cancer Treatments","authors":"Thomas Maho,&nbsp;Xavier Damany,&nbsp;Sébastien Dozias,&nbsp;Jean-Michel Pouvesle,&nbsp;Eric Robert","doi":"10.1016/j.cpme.2017.12.005","DOIUrl":"10.1016/j.cpme.2017.12.005","url":null,"abstract":"<div><p>Single plasma jets have allowed significant advances in <em>in vivo</em><span><span> or directly on human experiments (e.g. [1],[2]). The results are particularly promising, but ultimately likely to be limited in the future due to the fact that the treatment times are rather long due to the very small treated surface area resulting from the produced plasma. There is a real challenge to develop sources that allow treatment over larger areas while remaining practical and at a reasonable cost. There are already flexible or rigid surface DBDs but that require an extremely small distance between the reactor and the treated tissues, which limits their use in many situations, particularly when the treated surfaces exhibit large variations in the </span>surface morphology leading to points of attachment of streamers and therefore very inhomogeneous treatment. The jets are very useful in this type of situation because they adapt to all types of surfaces whether they are smooth or highly structured, or even if they have holes or cavities difficult to reach in the case of a surface plasma. On the other hand, floating DBDs are very sensitive to the nature of the treated substrate and the presence of seeps can prevent their operation. \"Ideal\" sources are therefore sources with the flexibility of a jet and surfaces comparable to large DBDs.</span></p><p>It is in this spirit that we have developed a new generation of applicators based on a single Plasma Gun system to generate a multitude of jets (from tens up to few hundreds) from a primary plasma jet, as shown in the photos below.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (263KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Before proceeding to in vivo treatments, we qualified the sources through <em>in vitro</em><span> experiments of decontamination on both colonies grown on agar plates in traditional Petri dishes, to check the equivalence of each of the jets generated, and colonies grown on very large agars scanned with our systems to treat very large surfaces in an easy way. The results are extremely encouraging and demonstrated the effectiveness of the multijet system, which allows both large-scale targets to be processed, as well as reducing processing times that can quickly become prohibitive with single-jet systems. At the same time as experimental results obtained on generation of different types of multijets, we will present results obtained with those on the decontamination of multi-resistant bacterial colonies grown from inpatient samples.</span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72912975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold Atmospheric Plasma for the treatment of Oral Lichen Planus as intraoral precancerous lesion","authors":"Christian Seebauer ,&nbsp;Sybille Hasse ,&nbsp;Maria Segebarth ,&nbsp;Sander Bekeschus ,&nbsp;Thomas von Woedtke ,&nbsp;Klaus-Dieter Weltmann ,&nbsp;Matthias Schuster ,&nbsp;Rico Rutkowski ,&nbsp;Hans-Robert Metelmann","doi":"10.1016/j.cpme.2017.12.069","DOIUrl":"10.1016/j.cpme.2017.12.069","url":null,"abstract":"<div><h3>Background</h3><p>Carcinogenesis of head and neck tumor<span> is a multiphase process that leads from the initial transformation of normal cells to a clinically manifest cancer with a latency of month to decades. Oral lichen planus<span><span> (OLP) is one of the common immune-mediated mucocutaneous disease characterized by chronic inflammatory process, in which an immune response attacks the lining epithelium. Several prospective and retrospective studies reported malignant transformation rates of OLP ranging from 0 to 10%. In this clinical study, we examined the therapeutic potential of Cold Atmospheric Plasma (CAP) for the </span>treatment of OLP as intraoral precancerous lesions.</span></span></p></div><div><h3>Material &amp; Methods</h3><p><span>For preclinical in-vitro investigations, tissue samples have been collected from healthy and diseased mucosal regions from 10 patients suffering from OLP. For detection of CAP-related cell death, investigation of </span>DNA fragmentation<span> using the TUNEL-assay has been performed after CAP treatment. Supernatant culture medium of tissue samples were analyzed for 13 different cytokines. Furthermore, white cell populations were stained in CAP-treated tissue samples. Saliva was collected for quantification of inflammatory markers. In addition, five patients suffering from intraoral leukoplakia of the cheek have undergone an in-vivo treatment using the plasma jet kINPen MED. Intraoral treatments have been performed for a period of 1 min/cm2 and with a continuous exhausting of gas and salivary.</span></p></div><div><h3>Results</h3><p>No significant enlargement of apoptotic cells could be detected after plasma exposure within the tissue. At the time this abstract was completed no final data concerning changes in cytokines as well as white cell population were available. The in-vivo treatment of OLP tissue revealed a reduction of pain and local inflammation. Especially the treatment of a therapy-resistant erosive OLP of the cheek could achieve pain reduction and a cure of mucosal ulcers.</p></div><div><h3>Conclusion</h3><p><span>Previous studies were primarily concerned with plasma effects on cancer cells<span> and its value in cancer treatment. This is the second report about plasma treatment of intraoral precancerous lesions. It has been shown that CAP could be useful in the treatment of intraoral mucosa diseases, especially OLP as precancerous lesion. The treatment of precancerous lesions could prevent the development of cancer and obviate the need for cancer treatment. A larger number of cases, investigation of long-term effects, and clinical studies are needed to further qualify plasma for treatment of precancerous lesions.</span></span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (424KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure shows the right cheek of a multimorbid ","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80397854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulating Plasma-Induced Thiol Chemistry In Liquids","authors":"Jan-Wilm Lackmann ,&nbsp;Christina Klinkhammer ,&nbsp;Christof Verlackt ,&nbsp;Helena Jabloniwski ,&nbsp;Friederike Kogelheide ,&nbsp;Katharina Stapelmann ,&nbsp;Annemie Bogaerts ,&nbsp;Martina Havenith ,&nbsp;Klaus-Dieter Weltmann ,&nbsp;Kristian Wende","doi":"10.1016/j.cpme.2017.12.060","DOIUrl":"10.1016/j.cpme.2017.12.060","url":null,"abstract":"<div><p><span>Cold physical plasmas are currently under investigation in various fields of industry and medicine. Clinical trials<span><span> using plasma for wound healing are well under way. In addition, investigations of plasmas for cancer treatment offer promising findings, too. However, the chemical interactions between plasmas and their biological targets are only partly understood. The complex chemical cocktails generated by plasma can affect various </span>biological structures [1]. A better understanding of these reactions would allow tuning and modulating plasmas for specific tasks, </span></span><em>e.g</em><span><span>. triggering wound healing or apoptosis cascades. One prevalent impact of plasma on biological targets is the chemical modification of </span>thiol groups<span>, which carry out various critical functions in the human body, such as cell signaling and protein structure formation. As thiols are involved in many regulatory and functional processes in tissues, an in-depth understanding of the impact of plasma treatment on thiols is highly relevant to optimize plasmas for medical applications.</span></span></p><p>To shed light onto these interactions, various thiol-containing model substrates were investigated with different plasma sources [2,3]. Using a normalized target substrate, the impact of the different plasma sources can be compared not by means of a physical characterization but by their chemical impact [4]. Stepwise increase of sample complexity allows monitoring how thiols are affected by plasma treatment in an ever more complex environment. The combination of experimental evidence and MD simulations permit a comprehensive overview of chemical processes induced by plasma treatment. This combined approach allows for a more throughout investigation of modifications on a molecular level and helps to understand fundamental plasma chemistry processes. Knowledge how different targets, ranging from small molecules to various proteins are affected by plasma treatment helps to understand how subsequent cellular responses can be triggered and what cross-reactions might be expected by plasma treatment.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.060","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76469932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface Dielectric Barrier Discharge Devices for Low Voltage and Low Power Radical Sheet","authors":"Seunghun Lee,&nbsp;Sunghoon Jung,&nbsp;Do-Geun Kim","doi":"10.1016/j.cpme.2017.12.007","DOIUrl":"10.1016/j.cpme.2017.12.007","url":null,"abstract":"<div><p><span>To improve the electrical stability and portability of surface dielectric<span><span> barrier discharge (SDBD) devices, it is necessary to secure low voltage and low power operations of SDBD. We fabricated polymer SDBD devices using commercial polymer substrates such as polyimide<span><span>, polyethylene terephthalate. Functional </span>thin film coatings were adapted to protect the degradation of polymer substrate due to </span></span>reactive oxygen radicals. And the optimization of the electrode structure was conducted to minimize displacement current flow and maximize dissipated power. Several types of electrode such as circular, rectangular, and hexagonal geometry were investigated for SDBD devices. The SDBD showed stable discharge at a low voltage of 2 kV or less, and a pulse frequency of 5-10 kHz. Design parameters of flexible SDBD devices for low voltage and low power operations will be presented.</span></span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (171KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1. Concept of radical sheet using surface dielectric barrier discharge<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (239KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 2. Radical sheet fabricated on polyethylene terephthalate substrate</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87649148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive Species Production In Argon And Helium Plasma Jets: Reactivity Transfer From Plasma To Liquid Layers","authors":"Natalia Babaeva *,&nbsp;George Naidis","doi":"10.1016/j.cpme.2017.12.052","DOIUrl":"10.1016/j.cpme.2017.12.052","url":null,"abstract":"<div><p><span>Atmospheric pressure plasma jets are often used in plasma medicine as sources of reactive species in biomedical applications including the treatment of wounds and cancerous tumors. A </span>rare gas or a mixture of a rare gas with a small percentage of oxygen are often used as a plasma-forming gases [1]. The biological responses are attributed to the production of reactive oxygen and nitrogen species. The reactive species generated by the plasma diffuse through a thin layer of a biological liquid which usually covers the treated sample.</p><p>In this work, we discuss results from computational investigations of properties of a single jet and jets array operated in He and Ar. We quantify densities and fluxes of reactive species produced by jets in the bulk plasma (Figure 1). We then investigate the reactivity transfer from the plasma into the liquid. As this process typically occurs on a large dynamic ranges of timescales, the plasma–liquid interactions and liquid phase chemistry are considered on a short (ns) and long (ms) time scales.</p><p>This investigation is conducted using the 2D modeling platform <em>nonPDPSIM,</em><span> which solves transport equations for charged and neutral species, Poisson’s equation for the electric potential, the electron energy conservation equation for the electron temperature and Navier–Stokes equations for the neutral gas flow. The model is essentially the same as used in [2]. The reaction mechanism includes plasma and neutral chemistry for He/humid air and Ar/humid air as well as liquid phase chemistry.</span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (388KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1. (a) and (c): Flow patterns for helium and argon jet into ambient air. Numbers at the lines show helium and argon mole fraction. (b) and (d): OH radicals production during a short plasma time scale for helium and argon jet, respectively.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.052","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83346412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Puublication Information","authors":"","doi":"10.1016/S2212-8166(18)30007-6","DOIUrl":"https://doi.org/10.1016/S2212-8166(18)30007-6","url":null,"abstract":"","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S2212-8166(18)30007-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137433548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Post-Graduate Education And Training Program For Participating In Trials And Treatment In Clinical Plasma Medicine","authors":"Ajay Rana ,&nbsp;Christian Seebauer ,&nbsp;Hans-Robert Metelmann","doi":"10.1016/j.cpme.2017.12.072","DOIUrl":"10.1016/j.cpme.2017.12.072","url":null,"abstract":"<div><p><span>Clinical studies and trials in plasma medicine require the adequate training of doctors and nurses so that they may use plasma technology in an effective and non-hazardous manner. The standardization and structuring of the training programs and education provides the most effective approach to consistent and reliable good quality in treatment. Since 1999, the </span><em>Diploma of Aesthetic Laser Medicine (D.A.L.M.)</em><span> has been the main university degree in this special field of laser medicine with a multicenter international teaching program [1]. The program remains independent from manufacturers and is based at Greifswald University in cooperation with the Deutsche Dermatologische Lasergesellschaft (DDL) in Germany and Institute of Laser &amp; Aesthetic Medicine (ILAMED) as an offshore campus in India for the English speaking countries.</span></p><p><span>With the introduction of licensed plasma medical devices in 2013 and demand for education, the curriculum has been revamped and extended in 2016. The extended </span><em>Diploma of Aesthetic Laser and Plasma Medicine,</em> representing the full range of today´s photonic therapy options, is certified by Greifswald University at an university degree level;. The program part concerning plasma medicine is based upon participation to (i) seminars (<em>Dies academicus)</em> with a focus on plasma medicine sciences and research, (ii) hands-on-training courses (<em>Hospitationes)</em>, putting an emphasis upon bed-side training, (iii) conferences featuring plasma medicine research (<em>Studium generale)</em>, for example at the meetings of the National Center of Plasma Medicine (NZPM) in Germany, at the conferences of the International Society of Plasma Medicine (ICPM) or at the annual International Workshop on Plasma for Cancer Treatment in (IWPTC) , and (iiii) finally presentation and discussion at the of the board examination (<em>Colloquium)</em> of fully documented cases of plasma treatment or scientific papers in the field of plasma medicine, prepared for publication in a peer-reviewed journal like <em>Clinical Plasma Medicine</em>.</p><p>Scientists with non-medical background involved or active in the field of plasma medicine research, in randomized clinical trials<span>, as members of the editorial board or as reviewers in relevant journals, and mainly interested in learning about the doctor´s view in clinical plasma medicine, are very much encouraged to join the program. They may proceed to their own selection of seminars and clinical courses in English or German. The same goes for nurses practicing in the field of cancer treatment and scientific assistants becoming involved in clinical plasma medicine trials. Separately from the extended D.A.L.M program there is an under-graduate lecture course “Introduction into Plasma Medicine” offered at Greifswald University Medicine.</span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91091423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Where Do Ros Go? Oxidation Cascades In Melanoma Exposed To Cold Physical Plasma","authors":"Katrin Rödder,&nbsp;Rajesh Gandhirajan,&nbsp;Thomas von Woedtke,&nbsp;Sander Bekeschus","doi":"10.1016/j.cpme.2017.12.046","DOIUrl":"10.1016/j.cpme.2017.12.046","url":null,"abstract":"<div><p><span>In plasma medicine, physical atmospheric cold plasma is used for application in therapeutic appliances such as wound healing or decontamination of infected skin.[1] Over the last decade, plasmas became a valuable research tool for cancer therapy.[2] Many groups have not only reported an efficient killing of cancer cells<span> with plasma but also a selectivity of the killing of malignant over non-malignant cells.[3] Yet, following the trajectories of plasma-derived reactive oxygen and nitrogen species has only begun to be explored. First modeling studies suggest an important role of lipid peroxidation<span><span> and phosphatidylserine content in the </span>cell membrane.[4] Using fluorescent reporter dyes, many groups have reported cytosolic </span></span></span>oxidation<span><span><span> following plasma treatment. Some groups claim that DNA double-stranded breaks are a direct product of reactive species deposition by plasmas.[5] Using human </span>melanoma cells, the purpose of this work was to study in detail how different </span>cell compartments<span> are affected reactive species derived the atmospheric pressure argon plasma jet kINPen. Using confocal laser scanning microscopy, several versions of fluorescent reporter dyes were used to assess oxidation qualitatively and quantitatively. We hope our study to contribute to the understanding how plasma-generated reactive species penetrate cells and affect their behavior and viability, especially for future plasma cancer treatment.</span></span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83752035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect Of Low-Temperature Plasma On Metastatic Processes Of Solid Tumors In Vitro","authors":"Angela Privat-Maldonado ,&nbsp;Evelien Smits ,&nbsp;Annemie Bogaerts","doi":"10.1016/j.cpme.2017.12.035","DOIUrl":"10.1016/j.cpme.2017.12.035","url":null,"abstract":"<div><p><span><span>Cancer metastasis is one of the leading causes of mortality </span>in patients<span> with solid tumors<span> [1]. The complex metastatic process involves cell migration and remodeling of the tumor microenvironment, among other factors. The intrinsic limitations of conventional cancer therapies for solid tumors have motivated the development and application of alternative technologies. In this context, the use of low-temperature plasmas (LTPs) has been explored </span></span></span><em>in vitro</em> and <em>in vivo</em><span><span> with considerable success against a variety of cancers [2,3]. However, whether LTP can inhibit or promote metastasis of solid tumors is unknown. The aim of this study is to investigate the effect of direct and indirect LTP treatments on metastatic processes of </span>glioblastoma<span><span><span><span>, adenocarcinoma and pancreatic cancers. For this purpose, we used a 3-dimensional multicellular </span>spheroid<span> model that mimics the pathophysiological conditions and complex architecture of solid tumors [4]. Spheroids were generated using glioblastoma U87, U251 and LN229, adenocarcinoma MDA-MB-231 and human </span></span>pancreatic carcinoma MIA PaCa-2 cell lines and glioblastoma primary cells GR04, GR08 and GR15. Direct and indirect plasma treatments were administered to spheroids in PBS solution using the COST plasma jet and kINPen IND. We used </span>live cell imaging<span><span> to assess cytotoxicity and spheroid morphology, microscopy to monitor cell migration and immunohistochemistry to assess </span>extracellular matrix remodeling in response to direct and indirect LTP treatment. Our study provides insight on the application of LTP treatment as potential cancer therapy for solid tumors.</span></span></span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (421KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>LN229 spheroids in PBS: (a) untreated control, directly treated with (b) kINPen IND or (c) COST jet. Outer border: total spheroid area; dark filled area: proliferative core (live cells); grey dots: dead cells.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.035","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73098564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}