Clinical Plasma Medicine最新文献

{"title":"Radiobiological Response Of Breast Cancer Cells, Exposed To Atmospheric Pressure Plasmas And Ionizing Radiation, An In-Vitro Essay. (Project Overview)","authors":"A.G. Cordero , S. Quirós , M. Porras , E. Avendaño","doi":"10.1016/j.cpme.2017.12.015","DOIUrl":"10.1016/j.cpme.2017.12.015","url":null,"abstract":"<div><p>The purpose of this project is to describe the application of an ionized gas to different lines of breast cancer cells<span>, added to application of a clinical photon beam, by measuring variations in cell death<span> rate. This procedure may lead to implementation of a new in vitro technique for studying new radiobiological approaches for adjuvant methods to radiotherapy techniques.</span></span></p></div><div><h3>Characterization of the plasma generator</h3><p><span>An in-house developed Cold Atmospheric Plasma \"CAP\" will be evaluated in terms of its functionality and features in geometry and operation. The characteristics of the plasma generated using an Argon-Helium gas mixture, should be analyzed regarding its possibilities of interaction with a biological medium, depending on properties such as the Power of the source; Gas pressure; Visible and infrared emission wavelength by FTIR Microscopy and Ultra-Violet (UVA, UVB, UVC) by UV absorption spectroscopy [1]; Gas temperature; Exposure time; Determination of reactive species, and </span>Gas flow (5 l/min).</p></div><div><h3>Biological Evaluations</h3><p><span>The cell lines of human breast adenocarcinoma<span><span> (MCF-7, MDA-MB-231, MDA-MB-468, Hs 578T, T-47D, MDA-N and BT-54), will be placed in plates of deposits with regular flat bottom for the application of ionizing radiation<span> and CAP, using a cell culture medium; RPMI 1640 supplemented with 10% fetal bovine serum, 100 units/ml </span></span>penicillin<span><span>, 100 μg/ml streptomycin and 0.25 μg/ml </span>amphotericin B, kept at 37 ° C, in an atmosphere humidified with 5% CO</span></span></span><sub>2</sub><span><span><span><span> and 95% air. In order to assess the cell viability at different radiation doses, in the presence and absence of </span>treatment<span> with CAP, the samples will be analyzed with the DNA binding<span> fluorochromes </span></span></span>Hoechst 33342<span> (slightly compromised cell membrane), and Propidium Iodide (complete compromised cell membrane). Images will be acquired with the multi-modal Cytation™3 microplate reader and analyzed for segmentation and quantification of the </span></span>cell nuclei for each of the fluorescent components, with Cell Profiler software.</span></p></div><div><h3>Dosimetric measurements</h3><p>For dose delivery, a beam of 6 MV from a Varian CLINAC iX (Varian Medical Systems, Palo Alto, California) will be used. This beam was calibrated in water following the protocol TECDOC-398. For the determination of dose levels, radio-chromic films (4x4 cm<sup>2</sup> samples) will be irradiated and measured for calibration using a spectrophotometer in the reflection mode [2].</p></div><div><h3>Expected results</h3><p><span>Cellular modifications produced by the combining of cold plasma characteristics and ionizing radiation, will generate a statistical sample that may allow us to determine the overall effect of modification in </span>radiosensitivity by the exposure to th","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 10-11"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90228583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immuno-modulatory effect of bio-plasma against cancer and other dreadful disease","authors":"Nagendra Kumar Kaushik * ,&nbsp;Neha Kaushik ,&nbsp;Manish Adhikari ,&nbsp;Su-Jae Lee ,&nbsp;Eun Ha Choi","doi":"10.1016/j.cpme.2017.12.024","DOIUrl":"10.1016/j.cpme.2017.12.024","url":null,"abstract":"<div><p><span><span>The application of plasma medicine technology has been actively explored over last several years. Recently, non-thermal plasma has demonstrated potential as a safe anticancer therapeutic approach that can kill various types of cancer targets [1-4]. There is the urgent need of new human health care’s technology against cancers based on immuno-modulations. Main target of our study is to enhance efficacy and selectivity of plasma on cancer cells<span> and cancer microenvironment by inducing immune-modulations. Fundamental insights on the cold plasma interactions with the immune cells<span><span> and cancer micro-environment can clarify the basic mechanisms of plasma induced immune-stimulation. Recent preliminary study suggests that plasma significantly modulated immune cells and can induce cancer cell death in co-culture condition [5-7]. In one of our recent studies, using a monocyte<span> cell model, we show that cold plasma activates the differentiation of monocytes into a macrophage-like phenotype and can increase mitochondrial/lysosomal numbers, suggesting that plasma may stimulate macrophage differentiation or activation. In turn, the interaction of plasma-activated macrophage cells reduced pro-tumorigenic features such as epithelial-mesenchymal-transition (EMT) and the stem-like population of tumor cells. Furthermore, plasma targeted macrophage activity and consequently enhanced </span></span>apoptosis via expression of the TNF-α/NOS+ M1-like phenotype. In another study we have evaluated role of plasma induced damage associated molecular patterns/ </span></span></span>immunogenic cell death<span><span> in immune activation and cancer treatment<span> in skin and oral cancer models. We have also initiated a study on plasma induced immuno-modulations for treatment of atopic dermatitis </span></span>skin disorder. Present research work mainly comprises plasma induced activation of immune cells; which find applications for curing various kinds of resistant tumors and other dreadful diseases. Our main objectives are (i) to clarify basic mechanism on plasma induced immuno-modulations (ii) to develop immunomodulation based strategy for the treatment of various dreadful diseases including cancers (iii) to initiate </span></span>clinical trial based on pre-clinical study.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 15"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.024","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73137666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution Of Intracellular Mobile Zinc To Anti-Cancer Effects Of Plasma-Activated Medium","authors":"Hirokazu Hara,&nbsp;Tetsuo Adachi","doi":"10.1016/j.cpme.2017.12.023","DOIUrl":"10.1016/j.cpme.2017.12.023","url":null,"abstract":"<div><p><span>Non-thermal plasma (NTP) irradiation has been reported to have a broad array of biological effects, and has been found to preferentially kill cancer cells<span>. Therefore, NTP is a promising approach for cancer therapy. The indirect NTP irradiation method using plasma-activated medium (PAM) induces cancer cell death<span> to the same extent as the direct NTP irradiation method. The anti-cancer effects of PAM are thought to be attributed to reactive oxygen species (ROS), mainly H</span></span></span>₂O₂<span>. On the other hand, oxidative stress caused by ROS has been demonstrated to induce the liberation of zinc (Zn</span>²⁺) from intracellular Zn²⁺ pools and Zn²⁺-dependent cell death. Zn²⁺ is thought to function as a second messenger activated by oxidative stress. We previously reported that PAM rapidly triggered intracellular Zn²⁺<span> liberation in human neuroblastoma SH-SY5Y cells, and that the liberated Zn</span>²⁺ induced activation of poly(ADP-ribose) polymerase 1 (PARP-1) and subsequent loss of cellular NAD⁺<span> and ATP, resulting in cell death associated with energy exhaustion. Recently, we also found that normal human fibroblasts were less sensitive to PAM cytotoxicity compared with SH-SY5Y cells. PAM decreased intracellular NAD</span>⁺ levels in both cell types, whereas the depletion of ATP and mitochondrial ROS generation were hardly observed in fibroblasts. The addition of Zn²⁺ augmented PAM-induced cell death and ATP depletion in fibroblasts. The levels of PAM-induced Zn²⁺ liberation were lower in fibroblasts than in SH-SY5Y cells. These findings suggest the possibility that cells with high intracellular mobile Zn²⁺<span> are susceptible to PAM cytotoxicity. Moreover, PAM promoted G2/M growth arrest of lung adenocarcinoma A549 cells via activation of the ATM-p53 pathway and the Zn</span>²⁺<span> chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) suppressed these events. Thus, we consider intracellular mobile Zn</span>²⁺ to play a key role in the anti-cancer effects of PAM.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 14-15"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83643369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold plasma treatment of in vitro gliomas and patient-derived tumors – Role of human myeloid cells","authors":"Sascha Marx ,&nbsp;Frederik Kinnen ,&nbsp;Juliane Moritz ,&nbsp;Eric Freund ,&nbsp;Mathias Stope ,&nbsp;Sandra Bien-Möller ,&nbsp;Bernhard H. Rauch ,&nbsp;Christopher Ritter ,&nbsp;Henry W.S. Schroeder ,&nbsp;Sander Bekeschus","doi":"10.1016/j.cpme.2017.12.009","DOIUrl":"10.1016/j.cpme.2017.12.009","url":null,"abstract":"<div><p><span><span>Tumor-associated macrophages (TAM) are the pre-dominant myeloid cells within </span>malignant glioma<span><span><span> and are a poor prognostic factor </span>in patients<span><span><span>. TAM in malignant glioma show a tumor-supporting, so-called M2 phenotype. The aim of this study was to characterize phenotype and relevant markers of monocytes/macrophages in the </span>tumor microenvironment following exposure to cold physical plasma Monocytes were enriched from </span>peripheral blood mononuclear cells derived from young healthy volunteers donating blood after </span></span>informed consent<span><span> and in accordance with the guidelines of the local ethics committee. Monocytes were co-cultured with a glioma cell line<span><span> (U87MG). The latter readily induced a M2 phenotype in these monocytes<span> as seen by an increase in CD163 expression. Prior to co-culture, either U87MG cells or monocytes were treated with an atmospheric pressure argon plasma jet (kINPen) for 15 seconds or 5 seconds, respectively. Non-treated co-cultures as well as single monocytes cultures served as controls. FACS immuno-phenotyping of monocytes/macrophages was performed for </span></span>CD55, CD97, CD162, CD163, CD169, CD204, CD276, HLA-DR, and HLA-ABC after 24h, 48h, and 72h. M2 polarization of monocytes by U87MG was neither attenuated nor reversed by plasma </span></span>treatment<span>. We next exposed primary glioblastoma patient material to cold physical plasma (kINPen) ex vivo. After treatment, tissues were incubated for 24h, and supernatants were collected. Multiplex cytokine analysis of supernatants. Results of five patients showed an absence of IFNα, IFNγ, IL12p70, IL17α, and IL23, and a presence of IL1β, TNFα, MCP1, IL6, IL8, IL10, IL18, and IL33 in samples. A mixed response was observed. For instance, both of the myeloid-cell interacting proteins MCP1 and IL1β are associated with glioma aggressiveness, and secretion of IL1β decreased after plasma treatment whereas MCP1 was increased. Glioma tissue sections will determine the distribution of myeloid cells within the tumor after plasma treatment compared to controls.</span></span></span></span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (136KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1: Fold change of several chemokines/cytokines in kINPen plasma-treated primary human glioblastoma samples compared to untreated glioma tissue.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 6-7"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.009","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84057020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold Atmospheric Plasma Suppresses Ovarian Cancer Cell Activity With Concurrent Secretion Of Heat Shock Protein 27 Affecting Monocyte Fate","authors":"Matthias B. Stope ,&nbsp;Dominique Koensgen ,&nbsp;Can Wulff ,&nbsp;Ilma Besic ,&nbsp;Denis Gümbel ,&nbsp;Anne Kaul ,&nbsp;Martin Weiss ,&nbsp;Karoline Diesing ,&nbsp;Axel Kramer ,&nbsp;Alexander Mustea ,&nbsp;Sander Bekeschus","doi":"10.1016/j.cpme.2017.12.040","DOIUrl":"10.1016/j.cpme.2017.12.040","url":null,"abstract":"<div><h3>Background</h3><p><span><span>Ovarian cancer (OC) is a gynecologic tumor with poor prognosis. Despite radical </span>cytoreductive surgery<span><span> and platinum-based adjuvant systemic treatment, </span>OC relapses<span> in the majority of the cases. Several studies have suggested profound antitumor activity of cold atmospheric plasma (CAP). CAP is an ionized gas that mainly mediates its effects via the release of a variety of reactive oxygen and nitrogen species. Evidence of CAP treatment on </span></span></span>OC cells is scarce, and accordingly we investigated their biological responses following exposure to plasma.</p></div><div><h3>Materials and Methods</h3><p><span><span><span>We tested the activity of CAP and CAP-treated medium to inactivate OC cell lines, and investigated their secretory products following CAP treatment. </span>Cell proliferation and </span>cell motility<span><span> was measured. The presence of HSP27 was measured by </span>Western blotting<span> and enzyme-linked immunosorbent assay (ELISA). Supernatants of plasma-treated OC cells were added to the human THP-1 monocytic cell line. Cellular oxidation, viability, growth, morphology, surface marker expression, and </span></span></span>cytokine release were monitored over up to 4 days of culture with supernatants of untreated and plasma-treated cancer cells.</p></div><div><h3>Results</h3><p><span><span>CAP treatment led to an attenuation of OC cell growth and motility. Incubation with CAP-treated cell culture medium gave similar effects. Results were consistent in four OC cell lines. Notably, CAP treatment of OC cells led to a decrease of intracellular HSP27 accompanied by an increase of extracellular HSP27 in the cell culture supernatant. These supernatants did not cause oxidation or blunted cell growth in THP-1 monocytes. Cell morphology was rather unaffected whereas from 12 </span>cell surface markers screened, two (CD271 and CD55) gave a significant increase in plasma-treated OC cell supernatants compared to untreated controls. HSP27 alone led to a decrease of both markers. Likewise, a screening of 13 cytokines release from THP-1 revealed a differential regulation of a number of targets, for example, an upregulation of </span>interleukin 8 in plasma-treated samples compared to untreated controls.</p></div><div><h3>Conclusion</h3><p>Plasma treatment was cytotoxic in ovarian cancer cell lines with functional and phenotypical consequences in human monocytes. The role of extracellular HSP27 induced by CAP treatment may represent immunomodulating mechanisms, which will be characterized more detailed in future.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 25-26"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87874756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Efficacy Of Plasma Activated Medium In 2d And 3d In Vitro Head And Neck Fadu Cancer Cell","authors":"Nofel Merbahi ,&nbsp;Julie Chaivin ,&nbsp;Florian Judée ,&nbsp;Patricia Vicendo ,&nbsp;Mohammed Yousfi","doi":"10.1016/j.cpme.2017.12.063","DOIUrl":"10.1016/j.cpme.2017.12.063","url":null,"abstract":"<div><p>Plasma Activated Media (PAM) has shown an increasing anticancer effect in the past few [1-3]. In <em>in vitro</em><span>, decrease in cell proliferation<span> [2.4], DNA damage [2;5] and apoptosis [6] were observed. The main advantage of PAM is its possibility to be prepared in advance and it can be used several weeks after activation by plasma jet when it is stored at the right temperature [2;7].</span></span></p><p><span>The aim of this work is to investigate the inhibitory effect of PAM and hydrogen peroxide H2O2 on Head and Neck cancerous cells (FaDu). The response of FaDu cells in monolayer cultures and Multi Cellular Tumor Spheroids<span> (MCTS) after treatment with different PAMs was compared. A homemade helium plasma jet was used to produce PAM. Helium plasma jet was generated using </span></span>dielectric<span> barrier discharge configuration. Helium was injected with flowmeter and the gas flow is fixed at 3L/min.</span></p><p>One treatment with PAM induces cell detachment from MTCs since the first day in a PAM exposure dependent manner. This is due to the presence of H2O2 in PAM. However, a rapid spheroids regrowth is observed attributed to a resistance of FaDu cells to H2O2. After multiple treatments of MCTS with PAM we obtained an inhibition of cell growth. MCTS is brought out with a comparison of PAM effect on 2D versus MCTS. Inversely, PAM induces cell death in the case of 2D cell culture. This result underlines the importance of working with MCTS instead of 2D cells.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 40-41"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75723344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma-Activated Medium Inhibites Metastatic Activities Of Ovarian Cancer Cells In Vitro Via Repressing Mapk Pathway","authors":"Yang Peng ,&nbsp;Hiroaki Kajiyama ,&nbsp;Kae Nakamura ,&nbsp;Fumi Utsumi ,&nbsp;Nobuhisa Yoshikawa ,&nbsp;Hiromasa Tanaka ,&nbsp;Masaaki Mizuno ,&nbsp;Shinya Toyokuni ,&nbsp;Masaru Hori ,&nbsp;Fumitaka Kikkawa","doi":"10.1016/j.cpme.2017.12.065","DOIUrl":"10.1016/j.cpme.2017.12.065","url":null,"abstract":"<div><p>Ovarian cancer is among the most malignant gynecologic cancers<span> since peritoneal dissemination often occurs at the diagnosis of these patients. The 5-year survival rate is less than 50%. Recently, non-equilibrium atmospheric pressure plasma (NEAPP) has been introduced in medical field. We have already demonstrated the cytotoxic effect of the direct plasma exposure to ovarian cancer cells. However, it is difficult to expose cancer cells to plasma gas intraperitoneally. Thus, we established the system of plasma-activated medium (PAM) to treat ovarian cancer indirectly instead of a direct exposure to plasma [1].</span></p><p>In our previous works, it was demonstrated that PAM significantly inhibited proliferation ability of ovarian cancer cells, with fibroblast cells remaining unaffected though. Both <em>in vitro</em> and <em>in vivo</em><span> study had confirmed the cytotoxic effect of PAM to ovarian cancer. However, it still remains unknown whether PAM can affect metastasis of ovarian cancer cells, which is the fatal problem of ovarian cancer patients [2].</span></p><p>In this study, we tried to investigate PAM’s anti-metastasis effect <em>in vitro</em><span><span> and the underneath mechanism. Firstly, we performed wound-healing and transwell assay on ES2, one of ovarian cancer cell lines. We found that the cell migration and invasion abilities were significantly inhibited by PAM. Secondly, we established a co-culture system by seeding ES2 cells onto monolayer of peritoneal </span>mesothelial cells<span>, which models the initial step of ovarian cancer cells metastasis in human peritoneal cavity<span>, and it was found that PAM significantly repressed ES2 cells to implant onto mesothelial cells. Moreover, mechanism study showed both mRNA and protein levels of MMP-9 were inhibited by PAM. And PAM significantly inhibited the phosphorylation of JNK1/2 MAPK and p38 MAPK (figure 1), which indicated that inhibition of MMP-9 was dependent on MAPK pathway [3].</span></span></span></p><p>In summary, it is indicated in this work that PAM presents efficient inhibitory effect towards ovarian cancer cells metastasis <em>in vitro</em><span>. Moreover, PAM’s anti-metastasis effect is implemented by repressing the activation MAPK pathway, resulting in de-activation of downstream target MMP-9, thus leading to suppression of cell migration and invasion. In the near future, it might be a new clinical strategy for metastatic ovarian cancer patients to choose PAM therapy via intaperitoneal treatment.</span><span><figure><span><img><ol><li><span>Download : <span>Download high-res image (86KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Fig. 1</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 41-42"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79434310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5th International Workshop on Plasma for Cancer Treatment (IWPCT 2018) Greifswald, Germany, 20-21 March 2018","authors":"","doi":"10.1016/j.cpme.2018.02.002","DOIUrl":"https://doi.org/10.1016/j.cpme.2018.02.002","url":null,"abstract":"","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 1"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2018.02.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137433547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold Atmospheric Plasma for the treatment of Oral Lichen Planus as intraoral precancerous lesion","authors":"Christian Seebauer ,&nbsp;Sybille Hasse ,&nbsp;Maria Segebarth ,&nbsp;Sander Bekeschus ,&nbsp;Thomas von Woedtke ,&nbsp;Klaus-Dieter Weltmann ,&nbsp;Matthias Schuster ,&nbsp;Rico Rutkowski ,&nbsp;Hans-Robert Metelmann","doi":"10.1016/j.cpme.2017.12.069","DOIUrl":"10.1016/j.cpme.2017.12.069","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;p&gt;Carcinogenesis of head and neck tumor&lt;span&gt; is a multiphase process that leads from the initial transformation of normal cells to a clinically manifest cancer with a latency of month to decades. Oral lichen planus&lt;span&gt;&lt;span&gt; (OLP) is one of the common immune-mediated mucocutaneous disease characterized by chronic inflammatory process, in which an immune response attacks the lining epithelium. Several prospective and retrospective studies reported malignant transformation rates of OLP ranging from 0 to 10%. In this clinical study, we examined the therapeutic potential of Cold Atmospheric Plasma (CAP) for the &lt;/span&gt;treatment of OLP as intraoral precancerous lesions.&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Material &amp; Methods&lt;/h3&gt;&lt;p&gt;&lt;span&gt;For preclinical in-vitro investigations, tissue samples have been collected from healthy and diseased mucosal regions from 10 patients suffering from OLP. For detection of CAP-related cell death, investigation of &lt;/span&gt;DNA fragmentation&lt;span&gt; using the TUNEL-assay has been performed after CAP treatment. Supernatant culture medium of tissue samples were analyzed for 13 different cytokines. Furthermore, white cell populations were stained in CAP-treated tissue samples. Saliva was collected for quantification of inflammatory markers. In addition, five patients suffering from intraoral leukoplakia of the cheek have undergone an in-vivo treatment using the plasma jet kINPen MED. Intraoral treatments have been performed for a period of 1 min/cm2 and with a continuous exhausting of gas and salivary.&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;No significant enlargement of apoptotic cells could be detected after plasma exposure within the tissue. At the time this abstract was completed no final data concerning changes in cytokines as well as white cell population were available. The in-vivo treatment of OLP tissue revealed a reduction of pain and local inflammation. Especially the treatment of a therapy-resistant erosive OLP of the cheek could achieve pain reduction and a cure of mucosal ulcers.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;p&gt;&lt;span&gt;Previous studies were primarily concerned with plasma effects on cancer cells&lt;span&gt; and its value in cancer treatment. This is the second report about plasma treatment of intraoral precancerous lesions. It has been shown that CAP could be useful in the treatment of intraoral mucosa diseases, especially OLP as precancerous lesion. The treatment of precancerous lesions could prevent the development of cancer and obviate the need for cancer treatment. A larger number of cases, investigation of long-term effects, and clinical studies are needed to further qualify plasma for treatment of precancerous lesions.&lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;figure&gt;&lt;span&gt;&lt;img&gt;&lt;ol&gt;&lt;li&gt;&lt;span&gt;Download : &lt;span&gt;Download high-res image (424KB)&lt;/span&gt;&lt;/span&gt;&lt;/li&gt;&lt;li&gt;&lt;span&gt;Download : &lt;span&gt;Download full-size image&lt;/span&gt;&lt;/span&gt;&lt;/li&gt;&lt;/ol&gt;&lt;/span&gt;&lt;/figure&gt;&lt;/span&gt;&lt;/p&gt;&lt;p&gt;Figure shows the right cheek of a multimorbid ","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 44-45"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80397854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microarry Analysis Identifies Activation Of Multiple Signalling Pathways In Primary Prostate Epithelial Cells By Low Temperature Plasma","authors":"John Packer ,&nbsp;Adam Hirst ,&nbsp;Fiona Frame ,&nbsp;Norman Maitland ,&nbsp;Deborah O’Connell","doi":"10.1016/j.cpme.2017.12.033","DOIUrl":"10.1016/j.cpme.2017.12.033","url":null,"abstract":"<div><p>We have previously reported the killing effects of Low Temperature Plasma (LTP) on primary prostate epithelial cells, of both normal and cancerous origin, via necrosis <strong>[1]</strong> following initial DNA damage. However it was evident that a population of cells was resistant to LTP treatment. To discern possible survival mechanisms in treated cells; the gene expression of 6 samples (4 cancers and 2 matched normal) was analysed 2 hours post-treatment, on a transcriptome wide level, by microarray. Initial analysis found alteration in the expression of 646 transcripts with significant activation of Notch, NF-kB, Nrf2 and AP-1 signalling in our primary cells. Pathway activation was subsequently validated by qRT-PCR in four different patient-derived prostate cultures to give a robust LTP-induced expression signature.</p><p>Upstream effector activation was then confirmed by immunofluorescence and Western blot. We found as other groups have; <strong>[2, 3]</strong> accumulation of Nrf2 in response to LTP, alongside AP-1 activation <strong>[4, 5]</strong> – through phosphorylation of both JNK and Jun. The Notch signalling pathway is typically described in relation to stem cell maintenance and we observed LTP induced Notch activation through release of its intracellular domain (NICD) that then acts as a nuclear transcription factor.</p><p>Tumours are innately heterogeneous and contain multiple cell populations, both intrinsic and recruited. We have established that our cultures contain three readily identifiable epithelial cell pools that can be separated to permit further analysis.<strong>[6]</strong> Preliminary investigation has found that the progenitor epithelial cells respond in greater measure than their more differentiated progeny, suggesting that they are more resistant to plasma. Similar observations of prostate stem cell resistance have been made with radiotherapy.<strong>[7]</strong> However, if these stem-like cells can be targeted – LTP may have a greater killing effect if utilized in prostate cancer treatments.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (151KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure – Pathway activation in primary prostate cancer epithelial culture; Nrf2 accumulation, AP-1 activation by JNK and Jun phosphorylation and Notch cleavage, with release of NICD by LTP. U – Untreated, T- Treated. Samples taken 0.5 and 2 hours following treatment with 3 minutes LTP.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Pages 20-21"},"PeriodicalIF":0.0,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77077545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}