Clinical Plasma Medicine最新文献

{"title":"Inhibition of murine melanoma tumor growth in vitro and in vivo using an argon-based plasma jet","authors":"Alireza Rafiei ,&nbsp;Farshad Sohbatzadeh ,&nbsp;Seyedehniaz Hadavi ,&nbsp;Sander Bekeschus ,&nbsp;Mina Alimohammadi ,&nbsp;Reza Valadan","doi":"10.1016/j.cpme.2020.100102","DOIUrl":"10.1016/j.cpme.2020.100102","url":null,"abstract":"<div><h3>Background</h3><p>In recent years, cold physical plasma gained significant interest as a new anticancer tool. Cold plasmas are versatile apparatuses in medicine and material processing. Among different cold plasma devices, the argon plasma jet is an effective apparatus and favorite for researches in plasma medicine science.</p></div><div><h3>Methods</h3><p><span>We developed an argon plasma jet for cancer treatments. Using optical emission spectroscopy<span><span>, the newly developed plasma jet produced a substantial amount of hydroxyl radicals and other reactive oxygen and nitrogen species known to show cytotoxic effects at higher concentrations. In this work, we studied the efficacy of the argon-based plasma jet treatment in murine B16 </span>melanoma cells </span></span><em>in vitro</em> and <em>in vivo. In vitro</em><span><span>, cell viability, apoptotic gene expression, and </span>cell death patterns were assessed at 24 h and compared against non-malignant murine dermal fibroblasts (MDF). </span><em>In vivo</em><span>, B16 cells inoculated into the flank of the mice developed melanoma tumors, which were plasma-treated for 5 min on days 1, 5, and 10 or were left untreated.</span></p></div><div><h3>Results</h3><p>Melanoma cells were significantly more sensitive to plasma treatment than MDF under the same <em>in vitro</em> conditions (p= 0.00023). The results revealed that the argon-based plasma jet damaged B16 melanoma cells <em>in vitro</em> selectively without a detrimental effect on non-malignant MDF cells. In the animal model, the tumor volume was significantly reduced after three treatments with the plasma jet(p&lt; 0.01).</p></div><div><h3>Conclusions</h3><p>The argon plasma jet showed therapeutic anti-melanoma efficacy <em>in vitro</em> and <em>in vivo</em>, which may spur new research lines in melanoma therapy.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100102","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81112859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of clinical applications of nitric oxide-containing air-plasma gas flow generated by Plason device","authors":"Alexandra V. Butenko ,&nbsp;Anatoly B. Shekhter ,&nbsp;Alexander V. Pekshev ,&nbsp;Andrey B. Vagapov ,&nbsp;Alexey L. Fayzullin ,&nbsp;Natalia B. Serejnikova ,&nbsp;Nikolay A. Sharapov ,&nbsp;Viktoria A. Zaborova ,&nbsp;Victor N. Vasilets","doi":"10.1016/j.cpme.2020.100112","DOIUrl":"10.1016/j.cpme.2020.100112","url":null,"abstract":"<div><h3>Background</h3><p><span>Nitric oxide<span> (NO) is a gaseous molecule produced by NO-synthases that regulates a wide range of physiological and pathophysiological processes. Endogenous NO signaling system plays an important role in all stages of wound healing. In 1998, a team from Bauman Moscow State Technical University and Sechenov First Moscow State Medical University (Sechenov University) developed the Plason device. Plason generates NO-containing air-plasma flow from atmospheric air and allows to apply required doses of NO to affected areas. It was shown, that exogenous NO penetrates through a wound surface, an intact skin, mucous membranes and a cornea, which leads to the wide range of applications. The experimental and clinical studies of 1992–2006 proved exogenous NO-therapy to be beneficial </span></span>in patients with extensive purulent wounds, trophic ulcers, diabetic foot syndrome, purulent-inflammatory disease of soft tissues and scars.</p></div><div><h3>Methods</h3><p>Up to now Plason is the only certified in Russia and Europe medical device<span> generating NO from atmospheric air at high therapeutically significant concentrations (500–1200 ppm). It is used in Russia, Ukraine, Belarus and Slovakia. Other air-plasma devices, “Tianox” (Sarov, Russia) and “iNO pulse” (Bellerophon Inc., USA) generated low concentrations of NO (10–100 ppm) were found effective against severe acute respiratory syndrome and recently tested for treatment of COVID-19. The purpose of this review is to acquaint global medical community with the extensive experience of Russian clinicians in NO-therapy. It covers the NO-therapy studies in multiple medical fields published after 2006.</span></p></div><div><h3>Results and conclusions</h3><p><span>Application of the Plason device was beneficial in treatment of wounds, purulent, traumatic and scar lesions, joint diseases<span><span>, including sports injuries, as well as in </span>abdominal surgery<span>, dentistry<span>, ophthalmology, </span></span></span></span>otolaryngology and other areas of medicine.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87068028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent effect of plasma-chemical NO-containing gas flow on wound healing. An experimental study","authors":"Anatoly B. Shekhter ,&nbsp;Alexander V. Pekshev ,&nbsp;Andrey B. Vagapov ,&nbsp;Alexandra V. Butenko ,&nbsp;Alexey L. Fayzullin ,&nbsp;Tatyana G. Rudenko ,&nbsp;Nikolay A. Sharapov ,&nbsp;Natalia B. Serejnikova ,&nbsp;Victor N. Vasilets","doi":"10.1016/j.cpme.2020.100101","DOIUrl":"10.1016/j.cpme.2020.100101","url":null,"abstract":"<div><h3>Background</h3><p><span><span>It is known that exogenous nitric oxide (NO) has a </span>bactericidal effect<span>, activates proliferation of fibroblasts, enhances angiogenesis and </span></span>collagen synthesis, accelerates maturation of granulation tissue.</p></div><div><h3>Objective</h3><p>The purpose of this research was to study the dose-dependent therapeutic effect of nitric oxide (NO) in NO-containing gas flows (NO-CGF) applied to the wound surfaces.</p></div><div><h3>Methods</h3><p>The experiment was performed in 36 white Wistar rats with full-thickness skin wounds with an area size of 3 cm²<span><span>. We used Plason device modified with an experimental plasma-chemical generator of nitric oxide (manipulator) for the following parameters of NO-CGF in the wound surface area: mass flow rate of nitric oxide 2.0 mg/s, axial gas flow velocity 9 m/s, nitric oxide content 1340 ppm, nitrogen dioxide content 108 ppm, gas flow temperature 39°C. Each group included 6 animals. The mass of nitric oxide delivered to the wound varied by the exposure time of 15, 30, 60, 120 and 360 seconds and was 30, 60, 120, 240 and 720 mg, respectively. Wounds were left untreated in the control group. Wound tissues were excised 4 days after the operation and studied with histological, semi-quantitative and </span>morphometric methods.</span></p></div><div><h3>Results</h3><p>Histological analysis revealed beneficial effects of NO therapy on wound healing, including reduction of microcirculatory disorders and exudate volume and acceleration of fibroblast proliferation and collagen synthesis. We proved that the most effective stimulation of the wound healing process occurred with a duration of exposure of 120 and 360 seconds (integral mass doses of NO on the wound surfaces were 240 mg and 720 mg, relative mass doses of NO per wound areas were 80 mg/cm² and 240 mg/cm², respectively). We did not identify the overdose phenomena after prolonged exposure of the wound to NO.</p></div><div><h3>Conclusion</h3><p>Our results are important for further improvement of the technique and the standardization of NO-therapy for the treatment of wounds, inflammatory and destructive processes in clinical practice.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85120540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breast cancer treatment using cold atmospheric plasma generated by the FE-DBD scheme","authors":"Ban H. Adil ,&nbsp;Ahmed Majeed Al-Shammari ,&nbsp;Hamid H. Murbat","doi":"10.1016/j.cpme.2020.100103","DOIUrl":"10.1016/j.cpme.2020.100103","url":null,"abstract":"<div><h3>Background</h3><p>Cold atmospheric plasma (CAP) is widely used in the cancer therapy field. This type of plasma is very close to room temperature. This paper illustrates the effects of CAP on breast cancer tissues both in vivo and in vitro.</p></div><div><h3>Methods</h3><p><span>The mouse mammary adenocarcinoma cell line AN3 was used for the in vivo study, and the MCF7, AMJ13, AMN3, and HBL cell lines were used for the in vitro study. A floating electrode-dielectric barrier discharge (FE-DBD) system was used. The cold plasma produced by the device was tested against breast </span>cancer cells.</p></div><div><h3>Results</h3><p>The induced cytotoxicity percentages were 61.7%, 68% and 58.07% for the MCF7, AMN3, and AMJ13 cell lines, respectively, whereas the normal breast tissue HBL cell line exhibited very little or no cytotoxicity. Reactive oxygen species<span> (ROS) were measured, and we found that more ROS were generated under the impact of CAP in cancer cells, whereas the normal HBL cell line had the lowest ROS level. The in vivo study showed that CAP treatment could reduce the volume of treated tumors compared to those in untreated mice.</span></p></div><div><h3>Conclusions</h3><p>CAP has anticancer effects both in vitro and in vivo and this effect is mediated by the ROS and induce apoptosis in p53 independent pathway. the current method is promising for breast cancer therapy.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100103","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85224249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold atmospheric pressure plasma treatment to assist the restoration of the apical region of a root canal in endodontic procedures","authors":"Alina Bisag ,&nbsp;Michele Manzini ,&nbsp;Emanuele Simoncelli ,&nbsp;Augusto Stancampiano ,&nbsp;Riccardo Tonini ,&nbsp;Matteo Gherardi ,&nbsp;Vittorio Colombo","doi":"10.1016/j.cpme.2020.100100","DOIUrl":"10.1016/j.cpme.2020.100100","url":null,"abstract":"<div><h3>Purpose</h3><p><span><span>In Endodontics, in order to complete a root canal </span>treatment, the use of guttapercha to completely seal the root apex must be coupled with endodontic cements known as sealers to improve the adhesion with dentine, despite their cytotoxicity and solubility. The present study investigates the effect of enhancement of adhesion between these materials and Cold Atmospheric Plasma (CAP)-treated dentine of the apical region of </span><em>ex-vivo</em> teeth as a first step towards the reduction on the use of cytotoxic materials and the use of CAP <em>in-vivo</em> at a clinical stage.</p></div><div><h3>Methods</h3><p><span>A dielectric barrier discharge (DBD) helium plasma jet was used to treat for 180 sec the shaped root canal dentin. Pushout tests and </span>confocal microscopy analysis have been performed to evaluate the effect of cold plasma treatment in terms of adhesion performances and interaction between filling materials and dentin respectively.</p></div><div><h3>Results</h3><p>The pushout test results highlight how a 180 seconds CAP treatment of dentin promotes an enhancement of bonding strength between filling materials and dentine in all investigated cases. In particular, when the dentine is CAP-treated in the apical region, the adhesion performances of guttapercha achieved a relevant increase (~ +200%), revealing how it was able to “self-bond” with the substrate with results comparable with the conventional procedure but without any sealer application, while the conventional (guttapercha+sealer) apical sealing procedure shows an improvement around +50%. Moreover, confocal images qualitatively confirmed the higher spreading and penetration of both guttapercha and sealer into dentinal tubules. The ergonomics of the prototype has been also improved to comply with the dimensions of devices commonly used in the dental field, with a plasma source diameter of 21.5 mm and with a choice of materials to minimise its weight.</p></div><div><h3>Conclusion</h3><p>CAP treatment of dentin increases the adhesion performances and paves the way for a sealer-free and safer procedure for apical restoration.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100100","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91210368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing the effectivity of the antimicrobial surface of carbon quantum dots-based nanocomposite by atmospheric pressure plasma","authors":"Mária Kováčová ,&nbsp;Michal Bodík ,&nbsp;Matej Mičušík ,&nbsp;Petr Humpolíček ,&nbsp;Peter Šiffalovič ,&nbsp;Zdenko Špitálsky","doi":"10.1016/j.cpme.2020.100111","DOIUrl":"10.1016/j.cpme.2020.100111","url":null,"abstract":"<div><p><span>Preventing nosocomial infections is one of the most significant challenges in modern medicine. The disinfection of medical facilities and medical devices<span> is crucial in order to prevent the uncontrolled spread of bacteria and viruses. Cost-effective, eco-friendly and fast-acting antibacterial coatings are being developed as the prevention of bacteria and viruses' multiplication on various surfaces. One of the possibilities to create such antimicrobial coatings can rely on a photoactive material, that produces </span></span>singlet oxygen<span><span>. However, a remote production of the singlet oxygen and disinfection of the desired surface is a time-consuming process. Hence, a coating material that would autonomously produce singlet oxygen employing ambient light will have a significant impact on the shortening of the disinfection time; leading into an increased number of patients that can be cured in one facility. In this work, an ultra-fast and eco-friendly method for decreasing the disinfection time of the photoactive surface is presented. The atmospheric pressure plasma surface treatment on the hydrophobic carbon quantum dots-polydimethylsiloxane nanocomposite is employed. The plasma-treated samples exhibited improved </span>antibacterial properties compared to non-plasma treated samples, with the best results obtained after only 30 seconds of plasma treatment. The short duration and the scalability potential of the here described method open new possibilities of how to improve the already existing antibacterial coatings.</span></p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75252695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold atmospheric plasma, the removal of blood from steel and its effect on staphylococcal biofilm formation. A pilot study","authors":"Muireann Fallon ,&nbsp;Maria Boyle ,&nbsp;Sarah Kennedy ,&nbsp;Stephen Daniels ,&nbsp;Hilary Humphreys","doi":"10.1016/j.cpme.2020.100104","DOIUrl":"10.1016/j.cpme.2020.100104","url":null,"abstract":"<div><h3>Background</h3><p>Cold atmospheric plasma (CAP) has antimicrobial properties and may have a role in decontamination. However, CAP can coagulate blood, which could lead to the fixing of blood or proteins and make surface decontamination challenging after blood spillages.</p></div><div><h3>Methods</h3><p><em>In vitro</em><span><span><span> assays were performed to assess whether CAP treatment of a surface impaired removal of a blood soil from steel through visual inspection and bicinchoninic acid assays. To assess if remaining </span>blood proteins promoted </span>biofilm<span> formation, crystal violet biomass analysis was performed.</span></span></p></div><div><h3>Results</h3><p>In an <em>in vitro</em> study, CAP treatment did not hinder the removal of blood and pre-treatment decreased protein remaining on the surface. CAP inhibited <span><em>Staphylococcus aureus</em></span> biofilm formation on blood-soiled surfaces.</p></div><div><h3>Conclusions</h3><p>CAP does not enhance the persistence of blood and protein on steel surfaces. Biofilm formation after CAP treatment of blood soiled steel was decreased possibly due to changes in electrostatic charge on surfaces. CAP should be further evaluated as an alternative surface decontaminant.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100104","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89202511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold atmospheric plasma (CAP) as a promising therapeutic option for mild to moderate acne vulgaris: Clinical and non-invasive evaluation of two cases","authors":"Dr. Arisi Mariachiara,&nbsp;Venturuzzo Anna,&nbsp;Gelmetti Alessandra,&nbsp;Guasco Pisani Edoardo,&nbsp;Bassissi Stefania,&nbsp;Rossi Mariateresa,&nbsp;Calzavara-Pinton Piergiacomo","doi":"10.1016/j.cpme.2020.100110","DOIUrl":"10.1016/j.cpme.2020.100110","url":null,"abstract":"<div><h3>Background</h3><p><span><span>Acne vulgaris is a multifactorial </span>skin disease that may be triggered by the presence of the bacteria </span>Cutibacterium acnes<span> and is characterized by an increase in the sebum excretion. Cold atmospheric plasma (CAP) represents an innovative technological device that uses plasma, the fourth state of matter, to treat several skin conditions, considering its bactericidal potential and its efficacy in promoting tissue proliferation.</span></p></div><div><h3>Objectives</h3><p><span>In the light of its well-known antibacterial properties and its promising regenerative properties, we assumed that CAP could be highly effective to treat </span>acne vulgaris both in its acute phase and in the long-term cicatricial period.</p></div><div><h3>Methods</h3><p><span>We reported the cases of two young patients affected by mild-moderate acne vulgaris of the face treated with CAP. In addition to the evaluation of inflammatory skin lesions<span>, treatment efficacy was assessed by sebometry and </span></span>trans epidermal water loss (TEWL) evaluation.</p></div><div><h3>Results</h3><p>In both patients, acne lesions reduced after treatment. Both sebum excretion parameters and TEWL improved considering basal values.</p></div><div><h3>Conclusion</h3><p>Despite the small number of patients enrolled, our data support CAP effectiveness in patients with mild to moderate acne vulgaris poorly responsive to other conventional treatments.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100110","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81413192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparison of two cold atmospheric helium plasma devices which utilise the same RF power generator","authors":"Cian D. Madigan ,&nbsp;Denis O'Sullivan ,&nbsp;Liam O'Neill ,&nbsp;Darren F. Kavanagh","doi":"10.1016/j.cpme.2020.100108","DOIUrl":"10.1016/j.cpme.2020.100108","url":null,"abstract":"<div><h3>Purpose</h3><p>This research was conducted to analyse and compare a Renuvion hand piece and TheraDep's deposition device at various gas flow rates and power settings. Both devices were powered by an Apyx Ultimate Electrosurgical generator.</p></div><div><h3>Methods</h3><p>Current, voltage, optical emission spectroscopy (OES) and thermal imaging were used to investigate the resulting plasma intensities and species being ionised. It was found that the Renuvion device could deliver 5.6 to 8.2 W of power whereas the Deposition Accessory was significantly less at between 1.9 and 4.2 W depending on the power setting selected. Differences in the OES results were also observed which are thought to have resulted from variations in the atmospheric entrainment between the two devices.</p></div><div><h3>Results</h3><p>Taken in combination, the electrical, optical emission and thermal data analysis has demonstrated that the addition of the Deposition Accessory transforms the Renuvion electrosurgical tool from a device that operates in the coagulation and cutting arena into a device with a dramatic reduction in the energy delivered to the target tissue. Consequently, this limits the localised heating of adjacent tissue and results in a device that has controlled coagulation capability and one which is not capable of cutting tissue.</p></div><div><h3>Conclusions</h3><p>This offers significant potential to allow clinicians to evaluate applications in plasma medicine using an FDA cleared electrosurgical power source.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100108","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80030460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canady cold plasma conversion system treatment: An effective inhibitor of cell viability in breast cancer molecular subtypes","authors":"Lawan Ly ,&nbsp;Xiaoqian Cheng ,&nbsp;Saravana R K Murthy ,&nbsp;Taisen Zhuang (Supervision) ,&nbsp;Olivia Z Jones (Writing – review and editing) ,&nbsp;Giacomo Basadonna (Supervision) ,&nbsp;Michael Keidar (Supervision) ,&nbsp;Jerome Canady (Conceptualization; Funding acquisition; Methodology; Supervision; Writing – review and editing)","doi":"10.1016/j.cpme.2020.100109","DOIUrl":"10.1016/j.cpme.2020.100109","url":null,"abstract":"<div><p>Breast cancer is a heterogenous disease which can be classified into subtypes by the presence or absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor 2 receptor (HER2). Cold atmospheric plasma (CAP) has been shown to be a potential treatment for cancer. In this report, a 92–99% reduction of viability by CAP treatment was achieved across all seven tested breast cancer cell lines (<em>p</em> ≤ 0.05). Increasing treatment duration and power significantly reduced breast cancer cell viability (** f_(2,2) ≤ 0.0176, *** f_(5,14) ≤ 0.0033). The authors are the first to report that breast cancer sensitivity to CAP is based on receptor status. Cells with identical receptor status showed the least difference in CAP sensitivity (<em>p</em> ≤ 0.05), the difference being 33% between the two ER+/PR+/HER2- cell lines (<em>p</em> ≤ 0.05) and 22–44% between the three TNBC cell lines (<em>p</em> ≤ 0.05). HER2-negative cell lines, irrespective of ER/PR status, also showed ≤ 50% difference in CAP sensitivity (<em>p</em> ≤ 0.05). Moreover, demonstration of ER-/PR-/HER2+ CAP susceptibility and ER+/PR+/HER2+ CAP resistance suggests that ER/PR status is a significant factor in determining CAP sensitivity in HER2-positive cells. Our novel findings on CAP sensitivity will provide insight on how to optimize CAP treatment to better overcome CAP resistance and thus prevent tumor recurrence.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2020.100109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76967771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}