非热血浆活化水对巨噬细胞抗癌免疫活性的影响

Q1 Medicine
Chae Bok Lee, Il Hwan Seo, Myoung-Won Chae, Jae Woo Park, Eun Ha Choi, Han Sup Uhm, Ku Youn Baik
{"title":"非热血浆活化水对巨噬细胞抗癌免疫活性的影响","authors":"Chae Bok Lee,&nbsp;Il Hwan Seo,&nbsp;Myoung-Won Chae,&nbsp;Jae Woo Park,&nbsp;Eun Ha Choi,&nbsp;Han Sup Uhm,&nbsp;Ku Youn Baik","doi":"10.1016/j.cpme.2017.12.049","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>Tumors can form tumor microenvironment<span> which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since </span></span>nitric oxide (NO) is known as a key regulator of </span>macrophage activation<span>, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells </span></span><em>in vitro</em> and <em>in vivo</em>. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (324KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N<sub>2</sub> and O<sub>2</sub> gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 31"},"PeriodicalIF":0.0000,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.049","citationCount":"2","resultStr":"{\"title\":\"Effects of Non-thermal Plasma Activated Water on the Anti-cancer Immune Activities of Macrophages\",\"authors\":\"Chae Bok Lee,&nbsp;Il Hwan Seo,&nbsp;Myoung-Won Chae,&nbsp;Jae Woo Park,&nbsp;Eun Ha Choi,&nbsp;Han Sup Uhm,&nbsp;Ku Youn Baik\",\"doi\":\"10.1016/j.cpme.2017.12.049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span>Tumors can form tumor microenvironment<span> which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since </span></span>nitric oxide (NO) is known as a key regulator of </span>macrophage activation<span>, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells </span></span><em>in vitro</em> and <em>in vivo</em>. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (324KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N<sub>2</sub> and O<sub>2</sub> gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells.</p></div>\",\"PeriodicalId\":46325,\"journal\":{\"name\":\"Clinical Plasma Medicine\",\"volume\":\"9 \",\"pages\":\"Page 31\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.049\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Plasma Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212816617300744\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Plasma Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212816617300744","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 2

摘要

肿瘤可形成肿瘤微环境,诱导肿瘤相关巨噬细胞(tumor associated macrophages, tam)从杀伤M1期向修复M2期转变。m2样tam不仅支持肿瘤生长和迁移,而且还阻止其他攻击肿瘤的免疫细胞的行动。巨噬细胞的这些功能是由多种因素介导的。由于一氧化氮(NO)被认为是巨噬细胞活化的关键调节剂,我们关注的是含NO的血浆活化水(NO- paw)作为重组tam的可能佐剂。在本研究中,我们发现NO-PAW上调M1型巨噬细胞激活,下调M2型巨噬细胞特性。检测M1或m2相关标记物的翻译和转录水平。此外,我们在体外和体内观察到NO-PAW增强了m2型巨噬细胞对黑色素瘤细胞的杀伤潜力。综上所述,本研究提示NO-PAW可诱导并维持肿瘤微环境下巨噬细胞的活化。下载:下载高分辨率图片(324KB)下载:下载全尺寸图片图1 (a)使用微波等离子体装置与N2和O2气体生成NO-PAW。(b) NO-PAW激活巨噬细胞,使其具有高杀伤黑色素瘤细胞的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Non-thermal Plasma Activated Water on the Anti-cancer Immune Activities of Macrophages

Tumors can form tumor microenvironment which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since nitric oxide (NO) is known as a key regulator of macrophage activation, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells in vitro and in vivo. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment.

  1. Download : Download high-res image (324KB)
  2. Download : Download full-size image

Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N2 and O2 gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Clinical Plasma Medicine
Clinical Plasma Medicine MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信