Chae Bok Lee, Il Hwan Seo, Myoung-Won Chae, Jae Woo Park, Eun Ha Choi, Han Sup Uhm, Ku Youn Baik
{"title":"非热血浆活化水对巨噬细胞抗癌免疫活性的影响","authors":"Chae Bok Lee, Il Hwan Seo, Myoung-Won Chae, Jae Woo Park, Eun Ha Choi, Han Sup Uhm, Ku Youn Baik","doi":"10.1016/j.cpme.2017.12.049","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>Tumors can form tumor microenvironment<span> which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since </span></span>nitric oxide (NO) is known as a key regulator of </span>macrophage activation<span>, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells </span></span><em>in vitro</em> and <em>in vivo</em>. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (324KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N<sub>2</sub> and O<sub>2</sub> gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":"9 ","pages":"Page 31"},"PeriodicalIF":0.0000,"publicationDate":"2018-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.049","citationCount":"2","resultStr":"{\"title\":\"Effects of Non-thermal Plasma Activated Water on the Anti-cancer Immune Activities of Macrophages\",\"authors\":\"Chae Bok Lee, Il Hwan Seo, Myoung-Won Chae, Jae Woo Park, Eun Ha Choi, Han Sup Uhm, Ku Youn Baik\",\"doi\":\"10.1016/j.cpme.2017.12.049\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span>Tumors can form tumor microenvironment<span> which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since </span></span>nitric oxide (NO) is known as a key regulator of </span>macrophage activation<span>, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells </span></span><em>in vitro</em> and <em>in vivo</em>. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment.<span><figure><span><img><ol><li><span>Download : <span>Download high-res image (324KB)</span></span></li><li><span>Download : <span>Download full-size image</span></span></li></ol></span></figure></span></p><p>Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N<sub>2</sub> and O<sub>2</sub> gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells.</p></div>\",\"PeriodicalId\":46325,\"journal\":{\"name\":\"Clinical Plasma Medicine\",\"volume\":\"9 \",\"pages\":\"Page 31\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.cpme.2017.12.049\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Plasma Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212816617300744\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Plasma Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212816617300744","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
Effects of Non-thermal Plasma Activated Water on the Anti-cancer Immune Activities of Macrophages
Tumors can form tumor microenvironment which educates tumor associated macrophages (TAMs) to change their identity from killing M1 stage to repair M2 stage. M2-like TAMs not only support tumor growth and migration but also prevent the action of other immune cells which attack tumors. Those functions of macrophages are mediated by many factors. Since nitric oxide (NO) is known as a key regulator of macrophage activation, we focused on NO-containing plasma activated water (NO-PAW) as a possible adjuvant to reorganize TAMs. In this study, we found that NO-PAW up-regulates M1 type macrophage activation and down-regulates M2 type macrophage characteristics. Translational and transcriptional levels of M1- or M2-related markers were examined. In addition, we observed that NO-PAW enhanced the killing potential of M2-type macrophages on melanoma cells in vitro and in vivo. In conclusion, our study suggests that NO-PAW induces and maintains activation of macrophages under tumor microenvironment.
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Figure 1 (a) NO-PAW was generated by using microwave-plasma device with N2 and O2 gases. (b) NO-PAW activated macrophages to have high potential to kill melanoma cells.