{"title":"血浆在眼表组织上的安全性评价:一项动物研究和组织病理学结果","authors":"Farhad Nejat, Nazanin-Sadat Nabavi, Mohammad-Amin Nejat, Hossein Aghamollaei, Khosrow Jadidi","doi":"10.1016/j.cpme.2019.100084","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>The plasma soft surgery is as an alternative to invasive surgical cosmetic procedures that can reduce the recovery time and possible complications after surgery. Due to the sensitivity of ocular surface tissue and the potential of the plasma, it can be evaluated to treat some ocular surface disorders. Accordingly, we evaluated the safety of the cold plasma on the ocular surface tissue in three areas located in the cornea, limbus, and conjunctiva.</p></div><div><h3>Methods</h3><p>Nine adult male New Zealand albino rabbits which divided into three groups were used for experiments. The left eye of each rabbit was chosen for test and the right eye was as the control. Experiments were performed on three ocular surface areas under the influence of the plasma. For this purpose, the plasma was created by Plexr device in continues mode and low power level (white handpiece; 0.7 W) was utilized at 0.7 s intervals using a 22-gauge needle. For evaluation of plasma safety, ocular surface integrity and histopathological changes were assessed after 24 h (A), one week (B), and one month (C) exposure to plasma.</p></div><div><h3>Results</h3><p><span><span>According to the external and slit-lamp examinations, after 24 h (A) only epithelial defect was visible while ocular surface in other groups (B & C) was healthy had completely recovered. </span>OCT imaging and histologic examination also confirmed these results. After 24 h conjunctival and corneal sections showed a localized area of epithelial loss together with </span>infiltration<span><span> of inflammatory cells<span> in the subepithelial stroma<span>, but during the one-month period, recovery was evident. Generally, after the first week, the loss of integrity of corneal epithelium has not been completely improved yet, while it was recovered nearly to be normal in group C. It was noteworthy that in all groups the loss of </span></span></span>endothelium cells was not apparent which confirming the lack of damage to the deep layers of the eye.</span></p></div><div><h3>Conclusion</h3><p>According to the plasma safety results, it seems that plasma soft surgery has the potential to be used as an alternative method for treatment of some of the ocular surface disorders without needing to conventional surgical procedures.</p></div>","PeriodicalId":46325,"journal":{"name":"Clinical Plasma Medicine","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.cpme.2019.100084","citationCount":"21","resultStr":"{\"title\":\"Safety evaluation of the plasma on ocular surface tissue: An animal study and histopathological findings\",\"authors\":\"Farhad Nejat, Nazanin-Sadat Nabavi, Mohammad-Amin Nejat, Hossein Aghamollaei, Khosrow Jadidi\",\"doi\":\"10.1016/j.cpme.2019.100084\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>The plasma soft surgery is as an alternative to invasive surgical cosmetic procedures that can reduce the recovery time and possible complications after surgery. Due to the sensitivity of ocular surface tissue and the potential of the plasma, it can be evaluated to treat some ocular surface disorders. Accordingly, we evaluated the safety of the cold plasma on the ocular surface tissue in three areas located in the cornea, limbus, and conjunctiva.</p></div><div><h3>Methods</h3><p>Nine adult male New Zealand albino rabbits which divided into three groups were used for experiments. The left eye of each rabbit was chosen for test and the right eye was as the control. Experiments were performed on three ocular surface areas under the influence of the plasma. For this purpose, the plasma was created by Plexr device in continues mode and low power level (white handpiece; 0.7 W) was utilized at 0.7 s intervals using a 22-gauge needle. For evaluation of plasma safety, ocular surface integrity and histopathological changes were assessed after 24 h (A), one week (B), and one month (C) exposure to plasma.</p></div><div><h3>Results</h3><p><span><span>According to the external and slit-lamp examinations, after 24 h (A) only epithelial defect was visible while ocular surface in other groups (B & C) was healthy had completely recovered. </span>OCT imaging and histologic examination also confirmed these results. After 24 h conjunctival and corneal sections showed a localized area of epithelial loss together with </span>infiltration<span><span> of inflammatory cells<span> in the subepithelial stroma<span>, but during the one-month period, recovery was evident. Generally, after the first week, the loss of integrity of corneal epithelium has not been completely improved yet, while it was recovered nearly to be normal in group C. It was noteworthy that in all groups the loss of </span></span></span>endothelium cells was not apparent which confirming the lack of damage to the deep layers of the eye.</span></p></div><div><h3>Conclusion</h3><p>According to the plasma safety results, it seems that plasma soft surgery has the potential to be used as an alternative method for treatment of some of the ocular surface disorders without needing to conventional surgical procedures.</p></div>\",\"PeriodicalId\":46325,\"journal\":{\"name\":\"Clinical Plasma Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.cpme.2019.100084\",\"citationCount\":\"21\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Clinical Plasma Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2212816618300465\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Plasma Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212816618300465","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 21
摘要
目的等离子体软性手术是一种可替代侵入性外科美容手术的方法，可缩短术后恢复时间，减少术后并发症。由于眼表组织的敏感性和血浆的潜力，它可以评估治疗一些眼表疾病。因此，我们评估了冷等离子体在角膜、角膜缘和结膜三个区域眼表组织上的安全性。方法选用成年雄性新西兰白化兔9只，分为3组进行实验。每只兔取左眼作试验，右眼作对照。在等离子体作用下，对三个眼表区域进行了实验。为此，等离子体由Plexr装置在连续模式和低功率电平下产生(白色耳机;0.7 W)，使用22号针，间隔0.7 s。为了评估血浆安全性，在24 h (A)、1周(B)和1个月(C)暴露于血浆后，评估眼表完整性和组织病理学变化。结果经体外及裂隙灯检查，24h后(A)只见上皮组织缺损，其余各组(B、p、p;C)健康已经完全康复。OCT成像和组织学检查也证实了这些结果。24 h后结膜和角膜切片显示局部上皮丢失，并在上皮下基质中浸润炎症细胞，但在一个月期间，恢复明显。一般情况下，第一周后角膜上皮完整性的丧失尚未完全改善，c组已基本恢复正常。值得注意的是，各组内皮细胞的丧失均不明显，证实眼深部未见损伤。结论血浆安全性试验结果表明，血浆软性手术有可能成为治疗某些眼表疾病的替代方法，而无需常规手术。
Safety evaluation of the plasma on ocular surface tissue: An animal study and histopathological findings
Purpose
The plasma soft surgery is as an alternative to invasive surgical cosmetic procedures that can reduce the recovery time and possible complications after surgery. Due to the sensitivity of ocular surface tissue and the potential of the plasma, it can be evaluated to treat some ocular surface disorders. Accordingly, we evaluated the safety of the cold plasma on the ocular surface tissue in three areas located in the cornea, limbus, and conjunctiva.
Methods
Nine adult male New Zealand albino rabbits which divided into three groups were used for experiments. The left eye of each rabbit was chosen for test and the right eye was as the control. Experiments were performed on three ocular surface areas under the influence of the plasma. For this purpose, the plasma was created by Plexr device in continues mode and low power level (white handpiece; 0.7 W) was utilized at 0.7 s intervals using a 22-gauge needle. For evaluation of plasma safety, ocular surface integrity and histopathological changes were assessed after 24 h (A), one week (B), and one month (C) exposure to plasma.
Results
According to the external and slit-lamp examinations, after 24 h (A) only epithelial defect was visible while ocular surface in other groups (B & C) was healthy had completely recovered. OCT imaging and histologic examination also confirmed these results. After 24 h conjunctival and corneal sections showed a localized area of epithelial loss together with infiltration of inflammatory cells in the subepithelial stroma, but during the one-month period, recovery was evident. Generally, after the first week, the loss of integrity of corneal epithelium has not been completely improved yet, while it was recovered nearly to be normal in group C. It was noteworthy that in all groups the loss of endothelium cells was not apparent which confirming the lack of damage to the deep layers of the eye.
Conclusion
According to the plasma safety results, it seems that plasma soft surgery has the potential to be used as an alternative method for treatment of some of the ocular surface disorders without needing to conventional surgical procedures.
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