APL Bioengineering最新文献_第8页

PEDOT:PSS-coated platinum electrodes for neural stimulation 用于神经刺激的 PEDOT:PSS 涂层铂电极

IF 6 3区医学

APL Bioengineering Pub Date : 2023-12-01 DOI: 10.1063/5.0153094

Gerwin Dijk, Jolien Pas, Katarina Marković, J. Ščančar, R. O'Connor

{"title":"PEDOT:PSS-coated platinum electrodes for neural stimulation","authors":"Gerwin Dijk, Jolien Pas, Katarina Marković, J. Ščančar, R. O'Connor","doi":"10.1063/5.0153094","DOIUrl":"https://doi.org/10.1063/5.0153094","url":null,"abstract":"Safe and long-term electrical stimulation of neurons requires charge injection without damaging the electrode and tissue. A common strategy to diminish adverse effects includes the modification of electrodes with materials that increases the charge injection capacity. Due to its high capacitance, the conducting polymer PEDOT:PSS is a promising coating material; however, the neural stimulation performance in terms of stability and safety remains largely unexplored. Here, PEDOT:PSS-coated platinum (Pt-PEDOT:PSS) microelectrodes are examined for neural stimulation and compared to bare platinum (Pt) electrodes. Microelectrodes in a bipolar configuration are used to deliver current-controlled, biphasic pulses with charge densities ranging from 64 to 255 μC cm−2. Stimulation for 2 h deteriorates bare Pt electrodes through corrosion, whereas the PEDOT:PSS coating prevents dissolution of Pt and shows no degradation. Acute stimulation of primary cortical cells cultured as neurospheres shows similar dependency on charge density for Pt and Pt-PEDOT:PSS electrodes with a threshold of 127 μC cm−2 and increased calcium response for higher charge densities. Continuous stimulation for 2 h results in higher levels of cell survival for Pt-PEDOT:PSS electrodes. Reduced cell survival on Pt electrodes is most profound for neurospheres in proximity of the electrodes. Extending the stimulation duration to 6 h increases cell death for both types of electrodes; however, neurospheres on Pt-PEDOT:PSS devices still show significant viability whereas stimulation is fatal for nearly all cells close to the Pt electrodes. This work demonstrates the protective properties of PEDOT:PSS that can be used as a promising approach to extend electrode lifetime and reduce cell damage for safe and long-term neural stimulation.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":" 7","pages":""},"PeriodicalIF":6.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138615361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FRESH™ 3D bioprinted cardiac tissue, a bioengineered platform for in vitro pharmacology. FRESH™3D生物打印心脏组织，体外药理学的生物工程平台。

IF 6 3区医学

APL Bioengineering Pub Date : 2023-12-01 DOI: 10.1063/5.0163363

Samuel Finkel, Shannon Sweet, Tyler Locke, Sydney Smith, Zhefan Wang, Christopher Sandini, John Imredy, Yufang He, Marc Durante, Armando Lagrutta, Adam Feinberg, Andrew Lee

{"title":"FRESH™ 3D bioprinted cardiac tissue, a bioengineered platform for in vitro pharmacology.","authors":"Samuel Finkel, Shannon Sweet, Tyler Locke, Sydney Smith, Zhefan Wang, Christopher Sandini, John Imredy, Yufang He, Marc Durante, Armando Lagrutta, Adam Feinberg, Andrew Lee","doi":"10.1063/5.0163363","DOIUrl":"10.1063/5.0163363","url":null,"abstract":"There is critical need for a predictive model of human cardiac physiology in drug development to assess compound effects on human tissues. In vitro two-dimensional monolayer cultures of cardiomyocytes provide biochemical and cellular readouts, and in vivo animal models provide information on systemic cardiovascular response. However, there remains a significant gap in these models due to their incomplete recapitulation of adult human cardiovascular physiology. Recent efforts in developing in vitro models from engineered heart tissues have demonstrated potential for bridging this gap using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in three-dimensional tissue structure. Here, we advance this paradigm by implementing FRESH™ 3D bioprinting to build human cardiac tissues in a medium throughput, well-plate format with controlled tissue architecture, tailored cellular composition, and native-like physiological function, specifically in its drug response. We combined hiPSC-CMs, endothelial cells, and fibroblasts in a cellular bioink and FRESH™ 3D bioprinted this mixture in the format of a thin tissue strip stabilized on a tissue fixture. We show that cardiac tissues could be fabricated directly in a 24-well plate format were composed of dense and highly aligned hiPSC-CMs at >600 million cells/mL and, within 14 days, demonstrated reproducible calcium transients and a fast conduction velocity of ∼16 cm/s. Interrogation of these cardiac tissues with the β-adrenergic receptor agonist isoproterenol showed responses consistent with positive chronotropy and inotropy. Treatment with calcium channel blocker verapamil demonstrated responses expected of hiPSC-CM derived cardiac tissues. These results confirm that FRESH™ 3D bioprinted cardiac tissues represent an in vitro platform that provides data on human physiological response.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"7 4","pages":"046113"},"PeriodicalIF":6.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10693443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138478917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel portable in situ printer for hydrogel multi-structure molding and cell printing 用于水凝胶多结构成型和细胞打印的新型便携式原位打印机

IF 6 3区医学

APL Bioengineering Pub Date : 2023-12-01 DOI: 10.1063/5.0176301

Huazhen Liu, Yi Zhang, Zhian Jian, Chuang Gao, Chunxiang Lu, Qiqi Dai, Hao Qiao, Yuanyuan Liu

{"title":"A novel portable in situ printer for hydrogel multi-structure molding and cell printing","authors":"Huazhen Liu, Yi Zhang, Zhian Jian, Chuang Gao, Chunxiang Lu, Qiqi Dai, Hao Qiao, Yuanyuan Liu","doi":"10.1063/5.0176301","DOIUrl":"https://doi.org/10.1063/5.0176301","url":null,"abstract":"Skin lesions not only disrupt appearance and barrier functionality but also lead to severe microbial infections and immune-inflammatory responses, seriously affect physical and mental health. In situ printing involves the direct deposition of bio-ink to create or repair damaged tissues or organs within a clinical setting. In this study, we designed and fabricated a novel portable in situ printer. This handheld instrument exhibits excellent printing performance, allowing hydrogels to be patterned and molded on surfaces according to specific requirements. By utilizing a dual-component hydrogels co-printing approach with high and low viscosities, we achieved in situ cell-laden printing using low-viscosity hydrogel. This demonstrates the advantages of the device in maintaining cell viability and achieving hydrogel structuring. This approach opens up the possibilities for the efficient encapsulation of active components such as drugs, proteins, and cells, enabling controlled macro- and micro-structuring of hydrogels. This breakthrough finding highlights the potential of our technical approach in dermatological treatment and wound repair, by dynamically adapting and regulating microenvironments in conjunction with hydrogel scaffolds and cell reparative impetus.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"6 1","pages":""},"PeriodicalIF":6.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138623466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEM2: Introducing mechanics in cell and tissue modeling using coarse-grained homogeneous particle dynamics SEM2：利用粗粒度均质颗粒动力学在细胞和组织建模中引入力学原理

IF 6 3区医学

APL Bioengineering Pub Date : 2023-12-01 DOI: 10.1063/5.0166829

Sandipan Chattaraj, Michele Torre, Constanze Kalcher, Alexander Stukowski, Simone Morganti, A. Reali, F. Pasqualini

{"title":"SEM2: Introducing mechanics in cell and tissue modeling using coarse-grained homogeneous particle dynamics","authors":"Sandipan Chattaraj, Michele Torre, Constanze Kalcher, Alexander Stukowski, Simone Morganti, A. Reali, F. Pasqualini","doi":"10.1063/5.0166829","DOIUrl":"https://doi.org/10.1063/5.0166829","url":null,"abstract":"Modeling multiscale mechanics in shape-shifting engineered tissues, such as organoids and organs-on-chip, is both important and challenging. In fact, it is difficult to model relevant tissue-level large non-linear deformations mediated by discrete cell-level behaviors, such as migration and proliferation. One approach to solve this problem is subcellular element modeling (SEM), where ensembles of coarse-grained particles interacting via empirically defined potentials are used to model individual cells while preserving cell rheology. However, an explicit treatment of multiscale mechanics in SEM was missing. Here, we incorporated analyses and visualizations of particle level stress and strain in the open-source software SEM++ to create a new framework that we call subcellular element modeling and mechanics or SEM2. To demonstrate SEM2, we provide a detailed mechanics treatment of classical SEM simulations including single-cell creep, migration, and proliferation. We also introduce an additional force to control nuclear positioning during migration and proliferation. Finally, we show how SEM2 can be used to model proliferation in engineered cell culture platforms such as organoids and organs-on-chip. For every scenario, we present the analysis of cell emergent behaviors as offered by SEM++ and examples of stress or strain distributions that are possible with SEM2. Throughout the study, we only used first-principles literature values or parametric studies, so we left to the Discussion a qualitative comparison of our insights with recently published results. The code for SEM2 is available on GitHub at https://github.com/Synthetic-Physiology-Lab/sem2.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":" 3","pages":""},"PeriodicalIF":6.0,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138613079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum: Publisher's Note: "Finite element analysis of electric field distribution during direct current stimulation of the spinal cord: Implications for device design" [APL Bioeng. 7, 046109 (2023)]. 勘误:出版商注:“在直流电刺激脊髓电场分布的有限元分析:对设备设计的启示”[APL生物工程，7,046109(2023)]。

IF 6 3区医学

APL Bioengineering Pub Date : 2023-11-22 eCollection Date: 2023-12-01 DOI: 10.1063/5.0187849

Joe G Troughton, Yaw O Ansong Snr, Nida Duobaite, Christopher M Proctor

引用次数: 0

OCT angiography in the monitoring of vaginal health. OCT血管造影术在阴道健康监测中的应用。

IF 6.6 3区医学

APL Bioengineering Pub Date : 2023-11-07 eCollection Date: 2023-12-01 DOI: 10.1063/5.0153461

Saijun Qiu, Afiba Arthur, Yuchen Jiang, Yusi Miao, Yan Li, Jingyi Wang, Yona Tadir, Felicia Lane, Zhongping Chen

{"title":"OCT angiography in the monitoring of vaginal health.","authors":"Saijun Qiu, Afiba Arthur, Yuchen Jiang, Yusi Miao, Yan Li, Jingyi Wang, Yona Tadir, Felicia Lane, Zhongping Chen","doi":"10.1063/5.0153461","DOIUrl":"10.1063/5.0153461","url":null,"abstract":"Fractional-pixel CO2 laser therapy shows promise for treating the genitourinary syndrome of menopause (GSM). Nevertheless, it remains controversial in the field of female pelvic medicine. This is due to the inherent difficulties in obtaining noninvasive biopsies to evaluate the treatment's efficacy and safety objectively. To address this challenge, we developed a noninvasive intravaginal optical coherence tomography (OCT)/OCT angiography (OCTA) endoscopic system, whose probe features a shape identical to the laser treatment probe. This system can provide high-resolution OCT images to identify the microstructure of vaginal tissue and visualize the vasculature network in vivo. We conducted clinical research on 25 post-menopausal patients with GSM. OCT/OCTA scans were acquired at four different locations of the vagina (distal anterior, distal posterior, proximal anterior, and proximal posterior) during the whole laser treatment session. A U-Net deep learning model was applied to segment the vaginal epithelium for assessing vaginal epithelial thickness (VET). Blood vessel density and VET were quantified to monitor the efficacy of fractional-pixel CO2 laser therapy. Statistical correlation analyses between these metrics and other clinical scores were conducted, validating the utility of our system. This OCT/OCTA endoscopic system has great potential to serve as a noninvasive biopsy tool in gynecological studies to screen, evaluate, and guide laser treatment for GSM.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"7 4","pages":"046112"},"PeriodicalIF":6.6,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10631816/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72016717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selective recording of physiologically evoked neural activity in a mixed autonomic nerve using a minimally invasive array. 使用微创阵列选择性记录混合自主神经中的生理诱发神经活动。

IF 6 3区医学

APL Bioengineering Pub Date : 2023-11-03 eCollection Date: 2023-12-01 DOI: 10.1063/5.0164951

Sophie C Payne, Peregrine B Osborne, Alex Thompson, Calvin D Eiber, Janet R Keast, James B Fallon

{"title":"Selective recording of physiologically evoked neural activity in a mixed autonomic nerve using a minimally invasive array.","authors":"Sophie C Payne, Peregrine B Osborne, Alex Thompson, Calvin D Eiber, Janet R Keast, James B Fallon","doi":"10.1063/5.0164951","DOIUrl":"10.1063/5.0164951","url":null,"abstract":"Real-time closed-loop control of neuromodulation devices requires long-term monitoring of neural activity in the peripheral nervous system. Although many signal extraction methods exist, few are both clinically viable and designed for extracting small signals from fragile peripheral visceral nerves. Here, we report that our minimally invasive recording and analysis technology extracts low to negative signal to noise ratio (SNR) neural activity from a visceral nerve with a high degree of specificity for fiber type and class. Complex activity was recorded from the rat pelvic nerve that was physiologically evoked during controlled bladder filling and voiding, in an extensively characterized in vivo model that provided an excellent test bed to validate our technology. Urethane-anesthetized male rats (n = 12) were implanted with a four-electrode planar array and the bladder instrumented for continuous-flow cystometry, which measures urodynamic function by recording bladder pressure changes during constant infusion of saline. We demonstrated that differential bipolar recordings and cross-correlation analyses extracts afferent and efferent activity, and discriminated between subpopulations of fibers based on conduction velocity. Integrated Aδ afferent fiber activity correlated with bladder pressure during voiding (r2: 0.66 ± 0.06) and was not affected by activating nociceptive afferents with intravesical capsaicin (r2: 0.59 ± 0.14, P = 0.54, and n = 3). Collectively, these results demonstrate our minimally invasive recording and analysis technology is selective in extracting mixed neural activity with low/negative SNR. Furthermore, integrated afferent activity reliably correlates with bladder pressure and is a promising first step in developing closed-loop technology for bladder control.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"7 4","pages":"046110"},"PeriodicalIF":6.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10625482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finite element analysis of electric field distribution during direct current stimulation of the spinal cord: Implications for device design. 脊髓直流电刺激过程中电场分布的有限元分析：对装置设计的启示。

IF 6 3区医学

APL Bioengineering Pub Date : 2023-11-02 eCollection Date: 2023-12-01 DOI: 10.1063/5.0163264

Joe G Troughton, Yaw O Ansong, Nida Duobaite, Christopher M Proctor

{"title":"Finite element analysis of electric field distribution during direct current stimulation of the spinal cord: Implications for device design.","authors":"Joe G Troughton, Yaw O Ansong, Nida Duobaite, Christopher M Proctor","doi":"10.1063/5.0163264","DOIUrl":"10.1063/5.0163264","url":null,"abstract":"Spinal cord injury (SCI) arises from damage to the spinal cord, often caused by trauma or disease. The resulting sensorimotor dysfunction is variable and dependent on the extent of the injury. Despite years of research, curative options for SCI remain limited. However, recent advancements in electric field stimulated axonal regrowth have shown promise for neuronal regeneration. One roadblock in the development of therapeutic treatments based on this is a lack of understanding of the exogenous electric field distribution in the injured tissue, and in particular, how this is influenced by electrode geometry and placement. To better understand this electric field, and provide a means by which it can be optimized, we have developed a finite element model of such spinal cord treatment. We investigate the impact of variations in electrode geometry, spinal cord size, and applied current magnitude as well as looking at several injury models in relation to clinically observed outcomes. Through this, we show that electrode shape has little effect on the induced electric field, that the placement of these electrodes has a noticeable influence on the field distribution, and that the magnitude of this field is governed by both the applied current and the spinal cord morphology. We also show that the injury modality influences the induced field distribution and that a stronger understanding of the injury will help decide treatment parameters. This work provides guidance in the design of electrodes for future clinical application in direct current electric field stimulation for axonal regeneration.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"7 4","pages":"046109"},"PeriodicalIF":6.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10624505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71487244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survivin as a mediator of stiffness-induced cell cycle progression and proliferation of vascular smooth muscle cells. Survivin作为僵硬诱导细胞周期进展和血管平滑肌细胞增殖的介质。

IF 6 3区医学

APL Bioengineering Pub Date : 2023-10-30 eCollection Date: 2023-12-01 DOI: 10.1063/5.0150532

John C Biber, Andra Sullivan, Joseph A Brazzo, Yuna Heo, Bat-Ider Tumenbayar, Amanda Krajnik, Kerry E Poppenberg, Vincent M Tutino, Su-Jin Heo, John Kolega, Kwonmoo Lee, Yongho Bae

{"title":"Survivin as a mediator of stiffness-induced cell cycle progression and proliferation of vascular smooth muscle cells.","authors":"John C Biber, Andra Sullivan, Joseph A Brazzo, Yuna Heo, Bat-Ider Tumenbayar, Amanda Krajnik, Kerry E Poppenberg, Vincent M Tutino, Su-Jin Heo, John Kolega, Kwonmoo Lee, Yongho Bae","doi":"10.1063/5.0150532","DOIUrl":"10.1063/5.0150532","url":null,"abstract":"Stiffened arteries are a pathology of atherosclerosis, hypertension, and coronary artery disease and a key risk factor for cardiovascular disease events. The increased stiffness of arteries triggers a phenotypic switch, hypermigration, and hyperproliferation of vascular smooth muscle cells (VSMCs), leading to neointimal hyperplasia and accelerated neointima formation. However, the mechanism underlying this trigger remains unknown. Our analyses of whole-transcriptome microarray data from mouse VSMCs cultured on stiff hydrogels simulating arterial pathology identified 623 genes that were significantly and differentially expressed (360 upregulated and 263 downregulated) relative to expression in VSMCs cultured on soft hydrogels. Functional enrichment and gene network analyses revealed that these stiffness-sensitive genes are linked to cell cycle progression and proliferation. Importantly, we found that survivin, an inhibitor of apoptosis protein, mediates stiffness-dependent cell cycle progression and proliferation as determined by gene network and pathway analyses, RT-qPCR, immunoblotting, and cell proliferation assays. Furthermore, we found that inhibition of cell cycle progression did not reduce survivin expression, suggesting that survivin functions as an upstream regulator of cell cycle progression and proliferation in response to ECM stiffness. Mechanistically, we found that the stiffness signal is mechanotransduced via the FAK-E2F1 signaling axis to regulate survivin expression, establishing a regulatory pathway for how the stiffness of the cellular microenvironment affects VSMC behaviors. Overall, our findings indicate that survivin is necessary for VSMC cycling and proliferation and plays a role in regulating stiffness-responsive phenotypes.","PeriodicalId":46288,"journal":{"name":"APL Bioengineering","volume":"7 4","pages":"046108"},"PeriodicalIF":6.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10618027/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71427789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigations on artificially extending the spectral range of natural vision. 人工扩大自然视觉光谱范围的研究。

IF 6 3区医学

APL Bioengineering Pub Date : 2023-10-24 eCollection Date: 2023-12-01 DOI: 10.1063/5.0156463

Abhijith Krishnan, C S Deepak, K S Narayan

引用次数: 0