Silk fibroin promotes H3K9me3 expression and chromatin reorganization to regulate endothelial cell proliferation.

IF 6.6 3区 医学 Q1 ENGINEERING, BIOMEDICAL
APL Bioengineering Pub Date : 2024-05-29 eCollection Date: 2024-06-01 DOI:10.1063/5.0203858
Kaixiang Gao, Yafan Xie, Fangning Xu, Qin Peng, Li Fu, Guixue Wang, Juhui Qiu
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引用次数: 0

Abstract

Silk fibroin (SF), which is extensively utilized in tissue engineering and vascular grafts for enhancing vascular regeneration, has not been thoroughly investigated for its epigenetic effects on endothelial cells (EC). This study employed RNA sequencing analysis to evaluate the activation of histone modification regulatory genes in EC treated with SF. Subsequent investigations revealed elevated H3K9me3 levels in SF-treated EC, as evidenced by immunofluorescence and western blot analysis. The study utilized H2B-eGFP endothelial cells to demonstrate that SF treatment results in the accumulation of H2B-marked chromatin in the nuclear inner cavities of EC. Inhibition of H3K9me3 levels by a histone deacetylase inhibitor TSA decreased cell proliferation. Furthermore, the activation of the MAPK signaling pathway using chromium picolinate decreased the proliferative activity and H3K9me3 level in SF-treated EC. SF also appeared to enhance cell growth and proliferation by modulating the H3K9me3 level and reorganizing chromatin, particularly after oxidative stress induced by H2O2 treatment. In summary, these findings indicate that SF promotes EC proliferation by increasing the H3K9me3 level even under stress conditions.

蚕丝纤维素促进 H3K9me3 表达和染色质重组,从而调节内皮细胞增殖。
蚕丝纤维素(SF)被广泛应用于组织工程和血管移植物中以促进血管再生,但其对内皮细胞(EC)的表观遗传学影响尚未得到深入研究。本研究利用 RNA 测序分析评估了经 SF 处理的内皮细胞中组蛋白修饰调控基因的激活情况。随后的研究发现,SF 处理的 EC 中 H3K9me3 水平升高,免疫荧光和 Western 印迹分析证明了这一点。该研究利用 H2B-eGFP 内皮细胞证明,SF 处理会导致 H2B 标记染色质在 EC 核内腔聚集。组蛋白去乙酰化酶抑制剂 TSA 可抑制 H3K9me3 水平,从而减少细胞增殖。此外,使用吡啶甲酸铬激活 MAPK 信号通路可降低 SF 处理的心肌细胞的增殖活性和 H3K9me3 水平。SF 似乎还能通过调节 H3K9me3 水平和重组染色质来促进细胞生长和增殖,尤其是在 H2O2 处理诱导的氧化应激后。总之,这些研究结果表明,即使在应激条件下,SF也能通过提高H3K9me3水平促进心肌细胞增殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
APL Bioengineering
APL Bioengineering ENGINEERING, BIOMEDICAL-
CiteScore
9.30
自引率
6.70%
发文量
39
审稿时长
19 weeks
期刊介绍: APL Bioengineering is devoted to research at the intersection of biology, physics, and engineering. The journal publishes high-impact manuscripts specific to the understanding and advancement of physics and engineering of biological systems. APL Bioengineering is the new home for the bioengineering and biomedical research communities. APL Bioengineering publishes original research articles, reviews, and perspectives. Topical coverage includes: -Biofabrication and Bioprinting -Biomedical Materials, Sensors, and Imaging -Engineered Living Systems -Cell and Tissue Engineering -Regenerative Medicine -Molecular, Cell, and Tissue Biomechanics -Systems Biology and Computational Biology
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