World Journal of Acupuncture-Moxibustion最新文献

{"title":"Thinking and practice of designing a single-blind, double-dummy clinical trial for acupuncture: Taking “comparative study of manual acupuncture and electroacupuncture for menopausal hot flush” as an example","authors":"Wen-ying SHI (石文英),&nbsp;Wei ZHANG (章薇)","doi":"10.1016/j.wjam.2023.09.001","DOIUrl":"https://doi.org/10.1016/j.wjam.2023.09.001","url":null,"abstract":"<div><p><span>Randomized controlled trial<span> (RCT) is the golden standard for evaluation of clinical evidence. To assess the effect of acupuncture, due to the specificity of acupuncture intervention, the blinding and control methods are the difficulties in study design. It is not practical to blind investigators, participants and evaluators simultaneously. At present, blinding is generally conducted for the participants and evaluators in </span></span>clinical trial<span><span>. We took a comparative study of manual acupuncture and electroacupuncture<span> for menopausal hot flush as an example to explore a new way with the reference of double-dummy design in new </span></span>drug study. A single-blind and double-dummy clinical trial model of acupuncture was established. Based on individual acupuncture intervention in each group, the placebo intervention was combined to ensure the form consistency of two groups. Such a study design may eliminate non-specific effect of acupuncture, and can be conductive to comparing the specific effect of acupuncture and can provide the references and regimens for study design.</span></p></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 4","pages":"Pages 367-371"},"PeriodicalIF":0.7,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92127486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Using evidence-based research approach and exploratory studies before conducting a new acupuncture randomized controlled trial","authors":"Xiao-yi HU (胡晓艺),&nbsp;Wei-juan GANG (岗卫娟),&nbsp;Xiang-hong JING (景向红)","doi":"10.1016/j.wjam.2023.09.004","DOIUrl":"https://doi.org/10.1016/j.wjam.2023.09.004","url":null,"abstract":"<div><p><span>Adequate preliminary research is a prerequisite for ensuring the quality of randomized controlled trials<span> (RCTs) on acupuncture. This paper proposes that prior to conducting a new RCT of acupuncture, an evidence-based research (EBR) approach and exploratory studies should be adopted to ensure value and avoid waste of research. EBR, defined as using </span></span>systematic reviews<span> of relevant clinical studies and obtain the perspectives of end users in a systematic and transparent way to justify research questions and inform trial design, is recommended as the first step before embarking on an acupuncture RCT to ensure its value and rationality. The subsequent exploratory studies can aid researchers in pre-evaluating the effect of acupuncture treatment protocols, identifying key factors that influence the outcome, and test trial feasibility for protocol optimization. This article elucidates the significance and implementation of the EBR approach and exploratory studies, with the aim of providing practical methods to enhance the quality of acupuncture RCTs.</span></p></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 4","pages":"Pages 325-327"},"PeriodicalIF":0.7,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92009447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A paradigm of medical-industrial integration: Application and prospects of new generation of intelligent technology in the parameter quantification for acupuncture manipulation","authors":"Chong SU (宿翀) ,&nbsp;Zi-yi CHEN (陈子燚) ,&nbsp;Pei-jing RONG (荣培晶)","doi":"10.1016/j.wjam.2023.04.002","DOIUrl":"10.1016/j.wjam.2023.04.002","url":null,"abstract":"","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 296-298"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41483030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penetration moxibustion for postpartum subinvolution of uterus: Two case reports and mechanism analysis","authors":"Wang-lu CHEN (陈王璐) ,&nbsp;Ling GAO (高崚) ,&nbsp;Xi-yan GAO (高希言)","doi":"10.1016/j.wjam.2023.03.003","DOIUrl":"10.1016/j.wjam.2023.03.003","url":null,"abstract":"<div><p><span><span>This article introduced the application of penetration moxibustion in successfully treating two cases of postpartum subinvolution of uterus. The main complaint of one case was increasing volume of abnormal </span>vaginal bleeding<span><span><span> for five days after delivery. The bleeding stopped after the patient received 7 times treatment of warming needle and penetration moxibustion on Zhōngjí (中极CV3), Guānyuán (关元CV4) and Qìhăi (气海CV6). The other case complained about persistent abdominal pain for 15 days, which was worsening for the recent 2 days. The symptom of abdominal pain was relieved after the penetration moxibustion was applied on CV4, CV6 and Zǐgōng (子宫 EX-CA1) for 5 times for the case. Penetration moxibustion can relieve abdominal pain during the </span>postpartum period, accelerate </span>lochia discharge, and recovery of menstrual and uterine cavity. </span></span><em>Àiyè</em> (艾叶 <em>Artemisiae Argyi Folium</em><span><span><span>) was proved to have antibacterial, hemostatic, and </span>analgesic effects. The warming function and radiation features of moxibustion can help to alleviate spasm of the </span>smooth muscle<span><span> in the uterus and enhance the metabolism of the uterine tissue by expanding microvessels and improving the circulations of both blood and lymph. It can also activate cells in the uterine tissue by increased temperature in order to enhance the decomposition and elimination of pathological wastes, such as inflammation and hematoma. In addition, it can also relieve </span>pelvic pain by inhibiting neuronal excitability.</span></span></p></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 287-292"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43188384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Moxibustion in prevention of post-hemorrhoidectomy urinary retention of mixed hemorrhoid: A randomized clinical trial","authors":"Jing GU (顾静) ,&nbsp;Li-jiang JI (季利江) ,&nbsp;Xin-yuan ZHANG (张馨元) ,&nbsp;Teng-da LI (李腾达) ,&nbsp;Hua HUANG (黄华) ,&nbsp;Jie JIANG (蒋捷) ,&nbsp;Mei ZHOU (周玫)","doi":"10.1016/j.wjam.2023.05.008","DOIUrl":"10.1016/j.wjam.2023.05.008","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the effect of moxibustion for preventing post-hemorrhoidectomy urinary retention of mixed hemorrhoids.</p></div><div><h3>Design</h3><p>Two-arm randomized clinical trial with blinded outcome assessment and statistician.</p></div><div><h3>Setting</h3><p>Anorectal Department of Changshu Affiliated Hospital of Nanjing University of Chinese Medicine, from October 2020 to a cutoff day in March 2021.</p></div><div><h3>Participants</h3><p>120 patients with mixed hemorrhoids undertaken hemorrhoidectomy.</p></div><div><h3>Interventions</h3><p>In the moxibustion group, moxibustion was exerted after surgery at Zhongji(CV3), Qihai (CV6), Guanyuan (CV4) and Sanyinjiao (SP6, bilateral). In the medication group, tamsulosin was administered orally after surgery. The cases were all observed for 24 h in two groups.</p></div><div><h3>Outcome measures</h3><p>The primary outcome was incidence of post-hemorrhoidectomy urinary retention and the secondary outcomes included time to the first voiding, the first voiding volume and adverse events were measured and compared between two groups.</p></div><div><h3>Results</h3><p>Sixty patients in each group were included in the data analysis and no change was made to the design of the trial after the trial begun. After treatment, the incidence of the post-hemorrhoidectomy urinary retention was 5% in the moxibustion group, lower than 20% in the medication group (<em>P</em> &lt; 0.05). The time to the first voiding after surgery was (4.76 ± 1.69) h in the moxibustion group, shorter than (6.81 ± 1.15) h in the medication group (<em>P</em> &lt; 0.05). The first voiding volume in the moxibustion group was (300.67±110.33)mL, higher than (224.67±90.88)mL in the medication group (<em>P</em>&lt;0.05) . There was no adverse event during study in the moxibustion group. Dizziness and nausea occurred in the medication group.</p></div><div><h3>Conclusion</h3><p>Moxibustion reduced the incidence of post-hemorrhoidectomy urinary retention, shortened the time to the first voiding and improved the urine volume after hemorrhoidectomy of mixed hemorroids. This therapy could be beneficial and safe, and thus could be recommended to the clinical practice.</p></div><div><h3>Trial registration</h3><p>Chi CTR2000039350.</p></div>","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 222-227"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48905234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of electroacupuncture with different frequencies for the treatment of headache and anxiety-depression symptoms in patients with migraine: A randomized clinical trial","authors":"Na NIE (聂娜) ,&nbsp;Qiong-ying SHEN (沈琼颖) ,&nbsp;Rui HUANG (黄睿) ,&nbsp;Si-ting YE (叶思婷) ,&nbsp;Le CHEN (陈乐) ,&nbsp;Chuan-long ZHOU (周传龙) ,&nbsp;Yi LIANG (梁宜)","doi":"10.1016/j.wjam.2023.05.006","DOIUrl":"10.1016/j.wjam.2023.05.006","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;p&gt;To observe the effects of electroacupuncture&lt;span&gt; (EA) with varied frequencies on headache and anxiety-depression symptoms in patients with migraine and to screen optimal frequency of EA for patients with such conditions.&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Design&lt;/h3&gt;&lt;p&gt;&lt;span&gt;Single-center, randomized, controlled clinical trial was designed, and the outcome assessors and statisticians were blinded. The patients with migraine were randomized into 2 Hz EA group, 100 Hz EA group and sham-stimulation group. In each group, the changes in migraine attacks, days with headache, the scores of visual analogy scale (VAS), self-rating anxiety scale (SAS), self-rating depression scale (SDS) and migraine-specific quality-of-life questionnaire (MSQ), as well as the dosage of &lt;/span&gt;analgesics&lt;span&gt; were observed at the baseline, during treatment, in 1, 2 and 3 months of follow-up separately.&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Setting&lt;/h3&gt;&lt;p&gt;Patients were enrolled in the Third Affiliated Hospital of Zhejiang Chinese Medical University, between 1&lt;sup&gt;st&lt;/sup&gt; August 2018 and 31&lt;sup&gt;st&lt;/sup&gt; July 2021.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Participants&lt;/h3&gt;&lt;p&gt;Sixty-five migraine patients with or without aura.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Interventions&lt;/h3&gt;&lt;p&gt;&lt;span&gt;In the EA groups, the acupoints were bilateral Fengchi (GB20), Gongxue (Extra), Sizhukong (TE23), Taiyang (EX-HN5), Shuaigu (GB8), Waiguan (TE5) and Yanglingquan (GB34). &lt;/span&gt;Electric stimulation&lt;span&gt; was exerted at GB20 and Gongxue (Extra), with 2 Hz and 100 Hz separately. In the sham-stimulation group, the shallow acupuncture was operated at the sites 1 cm lateral to GB20 and Gongxue (Extra), and on the radial side of TE5 and GB34. The output wires were cut off after attached to the acupoints. The patients in each group received the treatment 3 times weekly, once every two days, for consecutive 4 weeks. The complete intervention was composed of 12 treatments.&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Main outcome measures&lt;/h3&gt;&lt;p&gt;Changes in numbers of migraine attacks at treatment phase (week 1 to week 4) from the baseline(week -4 to week 0) in patients of each group&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;(1) Changes in migraine attacks and days with headache: In the 2 Hz EA and 100 Hz EA groups, the changes for migraine attacks and days of headache were higher significantly when compared with that in the sham-stimulation group at the same time stage (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). There was no statistical difference between two EA groups. (2) Changes of VAS score: In the 2 Hz EA and 100 Hz EA groups, the changes of VAS score were significantly higher when compared with that in the sham-stimulation group at the same time stage (&lt;em&gt;P&lt;/em&gt; &lt; 0.05). There was no statistical difference between two EA groups. (3) Assessment of anxiety and depression: The differences in the changes of SAS and SDS scores had no statistical significance in patients of each group at each assessment stage (&lt;em&gt;P&lt;/em&gt;&lt;span&gt; &gt; 0.05). (4) Assessment on the quality of life: Com","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 213-221"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43016796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalp acupuncture improves disordered behavior in the valproic acid-induced rat model of autism","authors":"Long-sheng HUANG (黄龙生) ,&nbsp;Pin GE (葛品) ,&nbsp;Jie KANG (康洁) ,&nbsp;Gui-hua LIU (刘桂华) ,&nbsp;Yu HONG (洪钰) ,&nbsp;Jing-rong WANG (王静蓉) ,&nbsp;Chu-an CHEN (陈楚安) ,&nbsp;Wan-yu ZHUANG (庄婉玉) ,&nbsp;Jing-min GUO (郭敬民) ,&nbsp;Ye-bin FENG (冯叶彬) ,&nbsp;Hong ZHANG (张宏) ,&nbsp;Ping OU (欧萍)","doi":"10.1016/j.wjam.2023.05.009","DOIUrl":"10.1016/j.wjam.2023.05.009","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objectives&lt;/h3&gt;&lt;p&gt;To investigate the underlying mechanisms of scalp acupuncture treatment (SAT) on autism spectrum disorder (ASD).&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;Thirty male Wistar rat pups that had been prenatally exposed to valproic acid sodium (VPA) were randomly divided into the VPA, VPA+acupoint, and VPA+ non-acupoint groups using the random number table method, with 10 rats in each group. Ten pups who had been prenatally exposed to saline were assigned to the control group (CG). There was no intervention in either the control or VPA groups. In the acupoint group, “Shenting (GV24),” bilateral “Benshen (GB13)” were manipulated. In the non-acupoint group, the area below the costal space was stimulated. Acupuncture stimulation lasted for 40 min, with manual twisting of the needles every 10 min, 5 days/week, with 2 days of rest per week, for a total duration of 4 weeks. After the corresponding treatments, behavioral tests (including the open field, social interaction, and Morris water maze tests) were performed to evaluate the therapeutic effects. RT-PCR and Western blotting were performed to detect the expression of neuregulin 1 (NRG1)/ErbB4.&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;In the open field test, the activity time spent in the central area in the VPA+acupoint group was significantly longer than that in the VPA group and VPA+ non-acupoint group (both &lt;em&gt;P&lt;/em&gt;&lt;0.05). The total length in the VPA+acupoint group was significantly longer than that in the VPA group (&lt;em&gt;P&lt;/em&gt;&lt;0.05). The number of bouts in the central area of the VPA+acupoint group was significantly higher than that of the VPA group (&lt;em&gt;P&lt;/em&gt;&lt;0.05). In session I of social interaction test, all experimental rats spent more time interacting with stranger 1 (all &lt;em&gt;P&lt;/em&gt;&lt;0.05). In session II, the CG and VPA+acupoint groups rats showed more interest in searching for new strangers, but the VPA+non-acupoint group spent more time interacting with stranger 1 than with stranger 2(all &lt;em&gt;P&lt;/em&gt;&lt;0.05). In the Morris water maze test, compared with the VPA group, the latency of the VPA+acupoint group was shorter (day 2, 3, 4, 5, &lt;em&gt;P&lt;/em&gt;&lt;0.05); compared with VPA+acupoint group, the latency of the VPA+non-acupoint group was longer (day 2, 4, &lt;em&gt;P&lt;/em&gt;&lt;0.05). The mean distance in the VPA+acupoint group was shorter than that in the VPA group (day 3, 5, &lt;em&gt;P&lt;/em&gt;&lt;0.05). The platform quadrant time of the VPA+non-acupoint group was significantly shorter than that of the VPA+acupoint group (&lt;em&gt;P&lt;/em&gt;&lt;0.05) (day 6). The VPA+acupoint group had more platform crossings than the VPA group (&lt;em&gt;P&lt;/em&gt;&lt;0.05), and the VPA+ non-acupoint group had fewer platform crossings than the VPA+acupoint group (&lt;em&gt;P&lt;/em&gt;&lt;0.05) (day 6). After SAT, the expression levels of NRG1 and ErbB4 proteins in the VPA+acupoint group were significantly increased than those in the VPA group (both &lt;em&gt;P&lt;/em&gt;&lt;0.05) in the medial prefrontal cortex (mPFC). The expression levels of N","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 252-261"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46336398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture at “Zusanli(ST36)” alleviates 5-fluorouracil-induced renal injury in colorectal cancer-bearing mice by exerting effects on oxidative stress, inflammatory responses, and cell apoptosis","authors":"Xue-jun ZHANG (张学君) ,&nbsp;Jiu-mao LIN (林久茂) ,&nbsp;Shi-lan CHEN (陈诗兰) ,&nbsp;Chen-jie LIN (林晨捷) ,&nbsp;Jiao PENG (彭娇) ,&nbsp;Xiao-dan YANG (杨晓丹) ,&nbsp;Jin-yan ZHAO (赵锦燕)","doi":"10.1016/j.wjam.2023.05.011","DOIUrl":"https://doi.org/10.1016/j.wjam.2023.05.011","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;p&gt;&lt;span&gt;To observe the effect of electroacupuncture at “Zusanli (ST36)” on 5-fluorouracil (5-FU)-induced renal injury in colorectal cancer-bearing mice, and to further investigate its effects on &lt;/span&gt;oxidative stress&lt;span&gt;, inflammatory responses, and cell apoptosis in mouse renal tissue.&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;&lt;span&gt;&lt;span&gt;Thirty-five male BALB/c mice were randomly divided into five groups of seven mice each, namely the control, CT26, 5-FU, sham point (SP), and ST36 (which received EA at the “ST36”) groups. With the exception of the control group, each group was subjected to establishment of a subcutaneous implantation tumor model using the murine CT26 colorectal cancer cell line. Once the models were successfully established, the 5-FU, SP, and ST36 groups received 5-FU injection solution intraperitoneally at a dose of 5 mg/mL once every three days over a 21-day period. Mice in the SP and ST36 groups additionally received an EA intervention after each intraperitoneal 5-FU injection. EA were performed on mice of the SP group at bilateral sham &lt;/span&gt;acupoints&lt;span&gt;&lt;span&gt; and on mice of the ST36 group at the bilateral “ST36” using the continuous wave mode at a frequency of 2 Hz for a duration of 5 min, intervention was administered once every two days for a duration of 21 days. Samples were collected from the mice at the end of the experiment. The pathological morphology of the renal tissue was observed using hematoxylin&lt;span&gt; and eosin (HE) staining; the contents of creatine (Cre), &lt;/span&gt;&lt;/span&gt;blood urea nitrogen (BUN) and &lt;/span&gt;&lt;/span&gt;malondialdehyde&lt;span&gt;&lt;span&gt;&lt;span&gt; (MDA), and superoxide dismutase (SOD) activity were measured using biochemical assays; the expression and nuclear translocation of nuclear factor kappa B p65 subunit (NF-κB p65) were measured by immunofluorescence; the expression levels of &lt;/span&gt;tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) in serum were measured by ELISA; cell apoptosis in renal tissue was detected using the &lt;/span&gt;TUNEL assay&lt;span&gt;; and the expression levels of the anti-B-cell lymphoma/leukemia 2 (Bcl-2), Bcl-2-associated X protein (Bax), cleaved caspase-3, cleaved caspase-9, and cytochrome C&lt;span&gt; (cyt c) in renal tissue were measured by Western blotting.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Compared with the control group, mice of the CT26 group showed a significant increase in serum Cre content (&lt;em&gt;P&lt;/em&gt;&lt;0.01), but the difference in BUN content was not statistically significant (&lt;em&gt;P&lt;/em&gt;&gt;0.05). HE staining of renal tissue revealed clear structures with normal glomerular and renal tubular morphology. SOD activity was decreased (&lt;em&gt;P&lt;/em&gt;&lt;0.01); MDA content was increased, but the increase was not statistically significant (&lt;em&gt;P&lt;/em&gt;&lt;span&gt;&gt;0.05). Differences in NF-κB p65 protein expression in the cytoplasm of renal tissue and serum levels of TNF-α, IL-6, and IL-1β were not statistically signifi","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 244-251"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49769605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Underlying mechnism of electroacupuncture for treating detrusor hyperreflexia after suprasacral spinal cord injury through the PACAP-cAMP signaling pathway","authors":"Ming XU (许明),&nbsp;Qiong LIU (刘琼),&nbsp;Shi-feng DENG (邓石峰),&nbsp;Ji-sheng LIU (刘继生),&nbsp;Ya LI (李亚),&nbsp;Zi-yuan JIAO (焦子远),&nbsp;Jing-zhi KUANG (匡静之),&nbsp;Kun AI (艾坤),&nbsp;Hong ZHANG (张泓)","doi":"10.1016/j.wjam.2023.06.002","DOIUrl":"10.1016/j.wjam.2023.06.002","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;p&gt;&lt;span&gt;&lt;span&gt;To elucidate the underlying mechanism and effect of electroacupuncture (EA) on the &lt;/span&gt;neurogenic bladder&lt;span&gt; following suprasacral spinal cord injury (SSCI). A rat model of &lt;/span&gt;&lt;/span&gt;detrusor&lt;span&gt; hyperreflexia&lt;span&gt;&lt;span&gt; after SSCI was established to examine the urodynamics, &lt;/span&gt;detrusor muscle&lt;span&gt; tissue morphology, the protein and mRNA expression levels of pituitary adenylate cyclase activating peptide&lt;span&gt; (PACAP) and its receptor PAC1R, and cyclic adenosine monophosphate (cAMP) content in the detrusor muscle with a focus on the PACAP-cAMP signaling pathway.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Method&lt;/h3&gt;&lt;p&gt;&lt;span&gt;A total of 72 female SD rats were randomized into control group and sham operation group (&lt;/span&gt;&lt;em&gt;n&lt;/em&gt;=12 per group) by using a random number table. The remaining 48 rats were established into the model of detrusor hyperreflexia after SSCI. After successful modeling, these rats were randomly assigned to model, EA, and EA + PACAP6-38 groups (&lt;em&gt;n&lt;/em&gt;&lt;span&gt;&lt;span&gt;&lt;span&gt;=12 per group). The unsuccessful modeled rats were used for exploratory observation. For the rats in EA group, “Ciliao (BL32)” “Zhongji (CV3)”, and “Sanyinjiao (SP6)” were needled and stimulated by EA. The PACAP receptor antagonist PACAP6-38 was administered intraperitoneally in the EA + PACAP6-38 group before EA, and EA was applied for seven consecutive days. After &lt;/span&gt;treatment&lt;span&gt;, the urodynamics of the rats were analyzed, and hematoxylin&lt;span&gt; and eosin staining was used to examine rat &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;bladder&lt;span&gt; detrusor tissue morphology. The expressions of PACAP-38 and PAC1R were detected by immunohistochemistry&lt;span&gt; and Western blot. The mRNA expression levels of PACAP-38 and PAC1R were examined by RT-qPCR, while cAMP content was detected by ELISA.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;&lt;span&gt;&lt;span&gt;(1) Compared with sham operation group, it was exhibited disarray in the transitional epithelium cells&lt;span&gt; of the bladder in the modeled rats. The intercellular space was significantly widened, accompanied by &lt;/span&gt;&lt;/span&gt;inflammatory cell infiltration and noticeable tissue edema. Both the bladder initial pressure and leak point pressure of the rats were higher (&lt;/span&gt;&lt;em&gt;P&lt;/em&gt;&lt;span&gt; &lt; 0.01), whereas the maximum cystometric capacity and bladder compliance were lower (&lt;/span&gt;&lt;em&gt;P&lt;/em&gt; &lt; 0.01). The protein and mRNA expression levels of PACAP-38 and PAC1R in the detrusor muscle, together with the cAMP content, were lower (&lt;em&gt;P&lt;/em&gt;&lt;span&gt;&lt;span&gt; &lt; 0.05). (2) Compared with the model rats, the EA group showed reduced inflammatory response in the detrusor muscle tissue, with decreased monocyte infiltration and less severe tissue edema. The bladder &lt;/span&gt;smooth muscle cells exhibited increased integrity, and there was decreased cellular tissue edema, inflammatory cell infiltration, and fibroplasia. The bladder initial pressure and leak point pressure were lower ","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 273-281"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47748758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of acupuncture on Beclin-1 and Caspase-3 expression in hippocampal tissue of rats with cerebral ischemia-reperfusion injury: Observations at different time points ☆基于不同时间点观察针刺对脑缺血再灌注损伤大鼠海马组织Beclin-1及Caspase-3表达的影响","authors":"Xiao-ye LU (卢小叶),&nbsp;Hui-e ZHENG (郑慧娥),&nbsp;Qian-yi LÜ (吕倩忆),&nbsp;Qi-long LI (李棋龙),&nbsp;Hong ZHANG (张泓),&nbsp;Hao-mei TIAN (田浩梅)","doi":"10.1016/j.wjam.2022.07.010","DOIUrl":"https://doi.org/10.1016/j.wjam.2022.07.010","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;p&gt;&lt;span&gt;To observe the changes in the neurological score, infarct size, and autophagy- and apoptosis-related indicators following the acupuncture treatment of rats with cerebral ischemia-reperfusion injury (CIRI) in order to investigate the effects of acupuncture on autophagy and &lt;/span&gt;apoptosis&lt;span&gt; in the hippocampal tissue of CIRI rats&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;p&gt;&lt;span&gt;In this study, 170 Sprague-Dawley rats were randomly assigned to the normal group (n=34), sham group (n=34), and model establishment group (n=102). A CIRI rat model was established in the model establishment group. The rats of the model establishment group were then randomly and equally allocated to the middle cerebral artery occlusion/reperfusion group (MCAO/R group), acupuncture group (AC group), and edaravone&lt;span&gt;&lt;span&gt; group (ED group) (n=34 each). Each group was further subdivided into the 24-h and 72-h subgroups (n=17 each). The rats of the MCAO/R group were immobilized for 30 min and administered with an intraperitoneal injection of 0.9% sodium chloride solution. The rats of the AC group were immobilized, and they received acupuncture at the“Dà zhuī (大椎 GV 14) ”,“Shuĭ gōu (水沟 GV 26 ) ”, and “Băi huì (百会 GV 20) ” and an intraperitoneal injection of 0.9% sodium chloride solution, with the &lt;/span&gt;acupuncture needles left in place for 30 min. The rats of the ED group were immobilized for 30 min and administered with an intraperitoneal injection of edaravone (5 mg/kg). All interventions were performed once every 12 h. Investigations of the neurological score, infarct size using &lt;/span&gt;&lt;/span&gt;triphenyl tetrazolium chloride&lt;span&gt;&lt;span&gt;&lt;span&gt; staining, hippocampal Caspase-3 protein expression by immunohistochemical assay, hippocampal Beclin-1 expression by &lt;/span&gt;Western blotting, &lt;/span&gt;autophagosomes&lt;span&gt; in the hippocampus&lt;span&gt;&lt;span&gt;&lt;span&gt; by transmission electron microscopy&lt;span&gt;, and apoptotic rate in the brain tissue of the ischemic side using the &lt;/span&gt;&lt;/span&gt;terminal deoxynucleotidyl transferase dUTP &lt;/span&gt;nick end labeling assay were performed both before and after the interventions.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;p&gt;Before intervention, the neurological score of the model establishment group was significantly lower than that of the normal and sham groups (&lt;em&gt;P&lt;/em&gt;&lt;0.01). After 24 h and 72 h, the neurological scores of the ED and AC groups were significantly increased (&lt;em&gt;P&lt;/em&gt;&lt;0.01), whereas the infarct size ratio were smaller (&lt;em&gt;P&lt;/em&gt;&lt;0.05 or &lt;em&gt;P&lt;/em&gt;&lt;0.01) and the apoptotic rates and Caspase-3 expression were lower (&lt;em&gt;P&lt;/em&gt;&lt;0.01) when compared with the MCAO/R group. The hippocampal Beclin-1 expression in the ED and AC groups was downregulated after 24 h of intervention (&lt;em&gt;P&lt;/em&gt;&lt;0.05) but upregulated after 72 h of intervention (&lt;em&gt;P&lt;/em&gt;&lt;0.05 and &lt;em&gt;P&lt;/em&gt;&lt;0.01, respectively). In the ED and AC groups, 72 h of intervention led to significant increases in the neurologi","PeriodicalId":44648,"journal":{"name":"World Journal of Acupuncture-Moxibustion","volume":"33 3","pages":"Pages 228-236"},"PeriodicalIF":0.7,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49795158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}