Sensors and Actuators Reports最新文献_第7页

A highly sensitive microfluidic biosensor for rapid and accurate detection of Salmonella in raw chicken products 用于快速准确检测生鸡肉产品中沙门氏菌的高灵敏度微流控生物传感器

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-11-08 DOI: 10.1016/j.snr.2024.100257

Mohammed Almalaysha , Arshdeep Singh , Sura A. Muhsin , Anna V. Carlson , Kate E. Trout , Amit Morey , Shuping Zhang , Lakshmikantha H. Channaiah , Mahmoud Almasri

{"title":"A highly sensitive microfluidic biosensor for rapid and accurate detection of Salmonella in raw chicken products","authors":"Mohammed Almalaysha , Arshdeep Singh , Sura A. Muhsin , Anna V. Carlson , Kate E. Trout , Amit Morey , Shuping Zhang , Lakshmikantha H. Channaiah , Mahmoud Almasri","doi":"10.1016/j.snr.2024.100257","DOIUrl":"10.1016/j.snr.2024.100257","url":null,"abstract":"<div><div>This paper presents an investigation of a fluidic-based impedance biosensor for rapid and accurate detection of <em>Salmonella</em> Typhimurium in raw chicken carcass rinsate. The biosensor is engineered with multiple distinct regions that concentrates <em>Salmonella</em> antigens to a detectable level, subsequently trapping the concentrated <em>Salmonella</em> samples on top of the detection interdigitated electrode array coated with a specific <em>Salmonella</em> antibody, maximizing the number of captured antigens. Detection is achieved through the antibody-antigen binding process, where binding events changes impedance values, providing a reliable method for identifying and quantifying <em>Salmonella</em>. The biosensor demonstrated a low limit of detection (LOD) of 1–2 cells/ml within 40–50 min. The findings demonstrated that the biosensor distinguishes low concentrations of live <em>Salmonella</em> cells, even in the presence of high concentrations of dead <em>Salmonella</em> cells, and non-specific binding pathogens viz.<em>, Listeria</em> monocytogenes and <em>E. coli O157:H7</em>.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100257"},"PeriodicalIF":6.5,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142652309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing nitrous oxide chemiresistive sensing performance by reducing ionic Oxygen species adsorption in Gold functionalized Tungsten Trioxide nanofibers 通过减少金功能化三氧化钨纳米纤维中的离子氧吸附，提高氧化亚氮化学电阻传感性能

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-11-04 DOI: 10.1016/j.snr.2024.100255

Dung Thi Hanh To, Bingxin Yang, Nosang Vincent Myung

{"title":"Enhancing nitrous oxide chemiresistive sensing performance by reducing ionic Oxygen species adsorption in Gold functionalized Tungsten Trioxide nanofibers","authors":"Dung Thi Hanh To, Bingxin Yang, Nosang Vincent Myung","doi":"10.1016/j.snr.2024.100255","DOIUrl":"10.1016/j.snr.2024.100255","url":null,"abstract":"<div><div>Low-cost nitrous oxide (N<sub>2</sub>O) gas sensor is in great need to provide real-time information to various stakeholders. Herein, various gold functionalized tungsten trioxide nanofibers (Au-WO<sub>3</sub> NFs) with different composition and crystallinity were synthesized by controlling electrospinning solutions and post heat treatment. These sensing materials were systematically exposed to various N<sub>2</sub>O concentrations at different operating temperatures (<em>i.e.,</em> 250 to 450 °C). Among different samples, 1 at % gold functionalized WO<sub>3</sub> nanofibers (1 at % Au-WO<sub>3</sub> NF) annealed at 600 °C for 24 h shows the highest sensitivity (<em>S</em> = R<sub>a</sub>/R<sub>o</sub>) of 38.5 toward 100 ppm at 250 °C with experimentally determined limit of detection (LOD) at 2.5 ppm. Although recovery and recovery time improved, the sensitivity reduced with an increase in operating temperatures. The detailed sensing mechanism studies indicated that the high N<sub>2</sub>O sensing was achieved when there were limited adsorbed ionized oxygen species (<em>e.g.,</em> O<sup>-</sup>). Moreover, N<sub>2</sub>O adsorption and desorption activation energy were estimated to be 0.13 and 0.87 eV where desorption was more strongly temperature dependent than adsorption.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100255"},"PeriodicalIF":6.5,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142652150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical review of hydrogen cyanide (HCN) sensors and their applications 氰化氢 (HCN) 传感器及其应用评述

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-23 DOI: 10.1016/j.snr.2024.100254

Dung Thi Hanh To, Nosang V. Myung

引用次数: 0

1D supramolecular assembly-induced emission and colorimetry toward precise onsite mercury(II) detection 一维超分子组装诱导发射和比色法实现现场汞（II）的精确检测

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-18 DOI: 10.1016/j.snr.2024.100253

Duan Xiong , Zhen Su , Lixin Zhang , Hao Zhang , Zhiwei Su , Xiaoyun Hu , Mailidan Wumaer , Qingqing Guan

{"title":"1D supramolecular assembly-induced emission and colorimetry toward precise onsite mercury(II) detection","authors":"Duan Xiong , Zhen Su , Lixin Zhang , Hao Zhang , Zhiwei Su , Xiaoyun Hu , Mailidan Wumaer , Qingqing Guan","doi":"10.1016/j.snr.2024.100253","DOIUrl":"10.1016/j.snr.2024.100253","url":null,"abstract":"<div><div>Manipulation of the one-dimensional (1D) supramolecular assembly of platinum(II) terpyridyl complex is promising for achieving precise onsite mercury(II) detection in complex environments, but still challenging. Herein, by systematic molecular design of platinum(II) terpyridyl complex, chloride-mediated 1D supramolecular assembly has been successfully achieved, exhibiting not only improved recognition ability but also dual-visual signal, with turn-on red luminescence and high-contrast color change from pale-yellow to orange-red. The probe also shows excellent selectivity and anti-interference properties, fast response rate (< 1 s) and low detection limit, stretching to 20.6 fg when incorporated in a hydrogel matrix. Structure insight for the dual-visual response shows that this high detection performance derives from the ancillary ligand of -NCS, which endows the increase of ion-association ability between platinum(II) terpyridyl complex and [HgCl<sub>4</sub>]<sup>2−</sup>, leading that 1D packing mode with strengthened Pt-Pt interactions. In all, this work highlights a new strategy of 1D supramolecular assembly construction for high performance detection of heavy metal ions.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100253"},"PeriodicalIF":6.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of human adenovirus by multiple cross displacement amplification combined with nanoparticle-based biosensor platform 通过多重交叉置换扩增结合纳米粒子生物传感器平台快速检测人类腺病毒

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-16 DOI: 10.1016/j.snr.2024.100252

Linglong Wan , Xiaolan Huang , Jin Fu , Fei Xiao , Nan Jia , Yu Zhang , Min Chen , Xiong Zhu , Shijun Li , Juan Zhou , Yi Wang

{"title":"Rapid detection of human adenovirus by multiple cross displacement amplification combined with nanoparticle-based biosensor platform","authors":"Linglong Wan , Xiaolan Huang , Jin Fu , Fei Xiao , Nan Jia , Yu Zhang , Min Chen , Xiong Zhu , Shijun Li , Juan Zhou , Yi Wang","doi":"10.1016/j.snr.2024.100252","DOIUrl":"10.1016/j.snr.2024.100252","url":null,"abstract":"<div><div>Human adenoviruses (HAdV), particularly serotypes 3 and 7 (HAdV-3 and HAdV-7), are significant respiratory pathogens that contribute to high morbidity rates and severe pneumonia in infants and children. The lack of distinct clinical presentations and effective treatments highlights the urgent need for rapid and reliable diagnostic methods for HAdV-3 and HAdV-7. This study devises a novel detection assay, termed HAdV-MCDA-LFB, which combines isothermal multiple cross displacement amplification (MCDA) with a nanoparticle-based lateral flow biosensor (LFB). Targeting the conserved hexon gene, HAdV-MCDA-LFB demonstrated high sensitivity, detecting low to 10 fg of hexon-containing plasmid per reaction without cross-reaction under the optimized conditions. Moreover, HAdV-MCDA-LFB exhibited comparable diagnostic accuracy to real-time PCR in clinical sample analysis, indicating its practical applicability. The whole procedure, including rapid template preparation (15 min), MCDA reaction (40 min at 67 °C) and result interpretation (<5 min), can be completed within one hour. Together, this rapid turnaround time, coupled with its simplicity and accuracy, makes HAdV-MCDA-LFB a promising point-of-care diagnostic tool for HAdV-3 and HAdV-7, particularly in resource-limited settings.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100252"},"PeriodicalIF":6.5,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142532674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of nanocrystallized magnetoelastic sensors with self-biased effect and improved mass sensitivity 开发具有自偏压效应和更高质量灵敏度的纳米结晶磁弹性传感器

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-11 DOI: 10.1016/j.snr.2024.100251

A. Lasheras , J.S. Garitaonandia , I. Quintana , J.L. Vilas , Ana Catarina Lopes

{"title":"Development of nanocrystallized magnetoelastic sensors with self-biased effect and improved mass sensitivity","authors":"A. Lasheras , J.S. Garitaonandia , I. Quintana , J.L. Vilas , Ana Catarina Lopes","doi":"10.1016/j.snr.2024.100251","DOIUrl":"10.1016/j.snr.2024.100251","url":null,"abstract":"<div><div>The growing demand for cost-effective and wireless sensing technologies requires the development of simple, efficient and optimized sensors able to accurately detecting external agents. Magnetoelastic resonators represent an alternative to the traditional sensing systems, able to combine all the previously cited factors. Several studies have focused on increasing their sensitivity, in order to make it closer to the market. The present study explores thermal treatments as a novel approach to enhance the sensitivity of magnetoelastic resonators, focusing on the positive impact of crystallization processes induced in magnetoelastic platforms. The experimental results confirm an enhancement of resonant frequency and quality factor of the magnetoelastic platforms as treatment temperature increases. Particularly, the sensor annealed at 550 °C shows an increase of the resonant frequency value of 45 % with respect to the <em>as-quenched</em> platform, being that increase of around 1700 % for the quality factor. In addition, the nanocrystallization induction leads to a self-biased resonance, consequence of the intrinsic magnetization resulting from the crystallization in Fe<sub>2</sub>B and FeCo phases. Further, the study shows the importance of stability in resonant frequency, emphasizing the potential of the 550 °C-annealed platform for mass sensor applications. Gold deposition experiments reveal the enhanced sensitivity of the sensor annealed at 550 °C of 40 % compared to the <em>as-quenched</em> sensor, as well as an increase of 38 % on its accuracy. These findings represent a significant step forward in the development of magnetoelastic-based mass sensors, highlighting the pivotal role of thermal treatments in optimizing sensitivity for practical and efficient external agents detection systems.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100251"},"PeriodicalIF":6.5,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible wide-range, sensitive three-axis pressure sensor array for robotic grasping feedback 用于机器人抓取反馈的灵活、宽范围、灵敏的三轴压力传感器阵列

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-10 DOI: 10.1016/j.snr.2024.100250

Minghui Yin , Yusen Guo , Jing An , TieZhu Liu , Guangyang Gou , Tong Li , Gang Chen , Tianjun Ma , Jianhai Sun , Mengdi Han , Jianqun Cheng , Jun Zhou , Xuan Sun , Chengyu Zhuang , Haiting Li , Chunxiu Liu , Zhimei Qi , Ning Xue

{"title":"Flexible wide-range, sensitive three-axis pressure sensor array for robotic grasping feedback","authors":"Minghui Yin , Yusen Guo , Jing An , TieZhu Liu , Guangyang Gou , Tong Li , Gang Chen , Tianjun Ma , Jianhai Sun , Mengdi Han , Jianqun Cheng , Jun Zhou , Xuan Sun , Chengyu Zhuang , Haiting Li , Chunxiu Liu , Zhimei Qi , Ning Xue","doi":"10.1016/j.snr.2024.100250","DOIUrl":"10.1016/j.snr.2024.100250","url":null,"abstract":"<div><div>Flexible pressure sensors capable of detecting normal and tangential forces through physical contact have garnered considerable interest in the realm of human-interactive systems. However, simultaneous detection of multi-directional forces is still a challenge for current research. Herein, a capacitive flexible pressure sensor based on a sandwich structure for three-dimensional force detection is proposed. The fabrication process of the sensor array is straightforward, capable of effectively distinguishing between normal and tangential forces. Polyimide (PI) serves as the flexible substrate for depositing the metal electrode pattern, while Polydimethylsiloxane (PDMS) acts as the intermediate dielectric layer material and the three-dimensional force conduction block. Through a comparative study of the thickness of the hollow dielectric layer, a pressure sensor with superior performance was prepared, featuring high sensitivity across a wide working range. Test results demonstrate its capability to detect normal forces ranging from 0 to 46 N (0–520 kPa) with a sensitivity of 0.442 N<sup>−1</sup> (0.031 kPa<sup>−1</sup>) and tangential forces from 0 to 10 N with a sensitivity of 0.08 N<sup>−1</sup> (X-axis) and 0.07 N<sup>−1</sup> (Y-axis). The designed acquisition system can simultaneously gather data from 6 sensor arrays, totaling 240 channels, with a response time of 11 ms. This sensor array, characterized by flexibility, versatility, and a wide range, is suitable for applications in robot tactile perception.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100250"},"PeriodicalIF":6.5,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-enzymatic glucose detection via ordered 2D arrays of nickel and nickel chitosan nanowires 通过镍和壳聚糖镍纳米线有序二维阵列进行非酶法葡萄糖检测

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-09 DOI: 10.1016/j.snr.2024.100249

Vicente Gonzales, Chris Rightsell, Alejandro Morales Betancourt, Kelly L. Nash

{"title":"Non-enzymatic glucose detection via ordered 2D arrays of nickel and nickel chitosan nanowires","authors":"Vicente Gonzales, Chris Rightsell, Alejandro Morales Betancourt, Kelly L. Nash","doi":"10.1016/j.snr.2024.100249","DOIUrl":"10.1016/j.snr.2024.100249","url":null,"abstract":"<div><div>Glucose sensors play a vital role in the everyday healthcare needs of diabetic patients. However, glucose sensor cost and reliability, particularly regarding their standard functionalization with expensive and environmentally sensitive enzymes, remains a challenge. A method for the fabrication of nickel nanowire arrays (NWAs) coated with a thin layer of chitosan for the non-enzymatic detection of glucose is herein reported. The method is based on the electrodeposition of nickel into anodic aluminum oxide (AAO) templates, followed by a novel chitosan coating procedure. The nickel and nickel chitosan NWAs were characterized via electron microscopy, Raman spectroscopy, and electrochemical techniques. Electrochemical testing using cyclic voltammetry and chronoamperometry demonstrated that the chitosan coating enhanced the selectivity and stability of the electrochemical sensor for glucose detection, even in the presence of interfering species. Additionally, the coating improved the sensor's sensitivity by 46.39 % and expanded its linear detection range from 3.85 mM to 4.37 mM. The chitosan coating also helped to retain these characteristics even after exposure to physiologically accurate samples and prevented biofouling after exposure to proteins. This simple and robust glucose sensor paves the way for the fabrication of glucose sensors with a high linear range without the need for functionalization with traditional glucose detecting agents such as glucose oxidase.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100249"},"PeriodicalIF":6.5,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Implementation and validation of MICaFVi: A highly efficient nanotechnology-based method for coronaviruses detection MICaFVi 的实施和验证：基于纳米技术的冠状病毒高效检测方法

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-03 DOI: 10.1016/j.snr.2024.100248

Nosaibah Samman , Haya A. Aljami , Sadeem Alhayli , Maha Alzayer , Khawlah Almuhalhil , Ahmad Alaskar , Sameera Aljohani , Atef Nehdi

{"title":"Implementation and validation of MICaFVi: A highly efficient nanotechnology-based method for coronaviruses detection","authors":"Nosaibah Samman , Haya A. Aljami , Sadeem Alhayli , Maha Alzayer , Khawlah Almuhalhil , Ahmad Alaskar , Sameera Aljohani , Atef Nehdi","doi":"10.1016/j.snr.2024.100248","DOIUrl":"10.1016/j.snr.2024.100248","url":null,"abstract":"<div><div>Coronaviruses have emerged as a significant public health concern due to the global impact of Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which cause COVID-19. The development of sensitive and accurate detection methods is critical for early diagnosis, disease management, and outbreak control. In a previous study, we developed and optimized a nanobased detection methodology called Magnetic Immuno-Capture Followed by Flow Virometry (MICaFVi) using virus-mimicking silica nanoparticles and MERS-CoV/SARS-CoV-2 pseudoviral particles. In the present study, we have extended this methodology to evaluate its specificity and sensitivity for detecting wild-type MERS-CoV and SARS-CoV-2 in human and camel samples. Our results demonstrated that MICaFVi successfully detected MERS-CoV and SARS-CoV-2 viruses with high sensitivity and specificity, although it showed reduced performance for samples with Ct values of 30 or lower compared to qPCR. Despite some limitations in detection speed and sensitivity, MICaFVi represents a significant advancement in diagnostic methodologies by combining nanotechnology with flow cytometry. Additionally, we adapted the MICaFVi methodology to simultaneously detect MERS-CoV and SARS-CoV-2 in a single multiplex assay. The successful implementation of this advanced detection approach has important implications for improving early detection, surveillance, and control of both current and future viral or bacterial pandemics. Our results underscore the potential of MICaFVi as a valuable tool for monitoring the spread of these viruses and highlight its role in advancing diagnostic technologies. This extension of our earlier work offers new insights into the application of nanotechnology and flow cytometry for viral detection.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100248"},"PeriodicalIF":6.5,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printable electrophoretic DNA extraction microdevice for on-site bacterial DNA recovery 用于现场细菌 DNA 提取的 3D 可打印电泳 DNA 提取微型装置

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-10-01 DOI: 10.1016/j.snr.2024.100244

Kiwon Nam , Seungbeom Kim , Younseong Song , Yoo Seok Lee , Seok Jae Lee , Kyoung G. Lee , Yong Tae Kim

{"title":"3D-printable electrophoretic DNA extraction microdevice for on-site bacterial DNA recovery","authors":"Kiwon Nam , Seungbeom Kim , Younseong Song , Yoo Seok Lee , Seok Jae Lee , Kyoung G. Lee , Yong Tae Kim","doi":"10.1016/j.snr.2024.100244","DOIUrl":"10.1016/j.snr.2024.100244","url":null,"abstract":"<div><div>Molecular diagnosis is a gold standard method for identifying an infectious disease. DNA extraction from a target pathogen is one of the most important procedures for accurate analysis of the disease-causative pathogen. In this study, a novel 3D-printed electrophoretic DNA extraction microdevice (3D-EDEM) was developed using a digital light processing-stereolithography (DLP-SL) for point-of-care analysis. The 3D-EDEM consists of a source chamber for a bacteria lysate reservoir, a sink chamber for an elution solution container, a hydrogel channel embracing capillary channels that act as a sieving matrix for size-based separation, and two electrode holders for supplying electrical current. Prior to fabricating the 3D-EDEM, UV-curable resin was prepared by using a poly(ethylene glycol) diacrylate (PEG-DA), Irgarcure 819 (IRG), and 2-isopropyl thioxanthone (ITX) as a monomer, a photoinitiator, and a photosensitizer, respectively. The 3D-printed 3D-EDEM provides numerous merits of being inexpensive, reproducible, and convenient, making it more suitable for on-site DNA extraction microdevices than soft-lithographic procedures. For DNA extraction on the 3D-EDEM, <em>Escherichia coli</em> O157:H7 (<em>E. coli</em>) lysate and elution buffer were loaded into the source chamber and the sink chamber, respectively. The optimum DNA extraction time and limit of the DNA extraction test of 3D-EDEM were carried out to evaluate DNA extraction performance, especially using a portable battery. Additionally, the successful DNA extraction test from artificially infected food samples confirms the applicability of the 3D-EDEM to real fields. The proposed 3D-EDEM is adequate for on-site DNA extraction in the field of clinical diagnosis, food safety, environmental monitoring, and forensic analysis.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100244"},"PeriodicalIF":6.5,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0