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Co-Polymer Sequence Determination Using SEC Combined with Py-GC SEC - Py-GC联合测定共聚物序列
LCGC Europe Pub Date : 2022-06-01 DOI: 10.56530/lcgc.eu.vo5772i2
W. Knol
{"title":"Co-Polymer Sequence Determination Using SEC Combined with Py-GC","authors":"W. Knol","doi":"10.56530/lcgc.eu.vo5772i2","DOIUrl":"https://doi.org/10.56530/lcgc.eu.vo5772i2","url":null,"abstract":"LCGC Europe spoke to Wouter Knol from the University of Amsterdam, The Netherlands, about co‑polymers, the difficulties surrounding their analysis, and what pyrolysis-gas chromatography (Py‑GC) offers in comparison to other techniques when analyzing co-polymers and their sequence.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122146800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 Cells 超临界流体色谱耦合漂移时间离子迁移率四极杆飞行时间质谱作为HepG2细胞脂质表征的工具
LCGC Europe Pub Date : 2022-06-01 DOI: 10.56530/lcgc.eu.xq5675w3
O. Schmitz, Sven W. Meckelmann, Pia Wittenhofer, Kristina Tštsch
{"title":"Supercritical Fluid Chromatography Coupled with Drift Time Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry as a Tool for Lipid Characterization of HepG2 Cells","authors":"O. Schmitz, Sven W. Meckelmann, Pia Wittenhofer, Kristina Tštsch","doi":"10.56530/lcgc.eu.xq5675w3","DOIUrl":"https://doi.org/10.56530/lcgc.eu.xq5675w3","url":null,"abstract":"Lipidomic studies are often conducted using shotgun mass spectrometry (MS) or reversed-phase liquid chromatography coupled with MS (LC–MS). However, chromatographic separation offers several advantages such as an additional identification parameter (retention time), lower ion suppression, and separation of isobaric species. In contrast, quantification is more difficult because ion suppression is not the same over the whole analysis, and as a consequence more standards are needed to compensate for this. Supercritical fluid chromatography (SFC) offers orthogonal separation compared to reversed-phase LC. While the separation of lipids in reversed-phase LC is mainly based on the length of the carbon chain and the number of double bonds, lipids in SFC are mainly separated according to their lipid classes, which simplifies quantification with standards. In this study, SFC coupled with drift time ion mobility quadrupole time-of-flight mass spectrometry (DTIMS-QTOF-MS)was used to characterize the HepG2 lipidome.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125304248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Taking the Red Pill: Alleviating Matrix Effects for Small Molecule Quantitation in Food and Feed 服用红色药丸:减轻食品和饲料中小分子定量的基质效应
LCGC Europe Pub Date : 2022-05-01 DOI: 10.56530/lcgc.eu.ot1884g4
R. Krska, M. Sulyok
{"title":"Taking the Red Pill: Alleviating Matrix Effects for Small Molecule Quantitation in Food and Feed","authors":"R. Krska, M. Sulyok","doi":"10.56530/lcgc.eu.ot1884g4","DOIUrl":"https://doi.org/10.56530/lcgc.eu.ot1884g4","url":null,"abstract":"Matrix effects continue to be an issue in small molecule quantitation in food analysis, with researchers exploring different approaches to circumvent the issue. LCGC Europe talked to Rudolf Krska and Michael Sulyok from the University of Natural Resources and Life Sciences (BOKU), Vienna, Austria, about their work to develop a multi-analyte approach using liquid chromatography tandem mass spectrometry (LC–MS/MS), and the issues surrounding agrocontaminants in animal feeds.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"98 10","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113975304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multivariate Optimization Procedure for Dynamic Headspace Extractions Coupled to GC(×GC) 气相色谱耦合的动态顶空提取多元优化程序(×GC)
LCGC Europe Pub Date : 2022-05-01 DOI: 10.56530/lcgc.eu.gi5670v6
Ryan P. Dias, Seo Lin Nam, Sheri A. Schmidt, A. P. de la Mata, J. Harynuk
{"title":"Multivariate Optimization Procedure for Dynamic Headspace Extractions Coupled to GC(×GC)","authors":"Ryan P. Dias, Seo Lin Nam, Sheri A. Schmidt, A. P. de la Mata, J. Harynuk","doi":"10.56530/lcgc.eu.gi5670v6","DOIUrl":"https://doi.org/10.56530/lcgc.eu.gi5670v6","url":null,"abstract":"Volatile organic compounds (VOCs) are ubiquitous chemicals of great interest in the study of aromas and flavours of foods. Many recent studies present optimized headspace (HS) and dynamic headspace (DHS) methods for specific sample types; however, the literature does not present ­(to the best of our knowledge) a generalized procedure for the thorough optimization of a DHS extraction. This article presents an approach using design of experiments (DoE) for the optimization of DHS extraction parameters. The approach is demonstrated for two different food sample types with diverse populations of VOCs: active sourdough colony as an example with a high moisture content, and sourdough bread as an example with a lower moisture content. Optimized methods are assessed for VOC extraction reproducibility and exhaustiveness; guidelines for DHS optimization are presented.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126038364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Supercritical Fluid Chromatography for Chiral Analysis, Part 2: Applications 手性分析用超临界流体色谱，第2部分:应用
LCGC Europe Pub Date : 2022-04-01 DOI: 10.56530/lcgc.eu.fn8374q5
G. Roskam, Bas van de Velde, Andrea F. G. Gargano, Isabelle Kohler
{"title":"Supercritical Fluid Chromatography for Chiral Analysis, Part 2: Applications","authors":"G. Roskam, Bas van de Velde, Andrea F. G. Gargano, Isabelle Kohler","doi":"10.56530/lcgc.eu.fn8374q5","DOIUrl":"https://doi.org/10.56530/lcgc.eu.fn8374q5","url":null,"abstract":"In the second part of this review article, the recent progress in supercritical fluid chromatography (SFC) for enantiomeric separations is evaluated. With the substantial developments carried out over the past years in instrumentation, columns, and detector hyphenation, the interest in chiral SFC has been steadily growing in various fields. In combination with novel developments in chiral stationary phase chemistries, the enantioselective analysis range has been significantly extended. Several applications reported on the enantioselective separation of drugs and pharmaceutical compounds using chiral SFC are discussed, including pharmaceutical applications, clinical research, forensic toxicology, and environmental sciences.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131301238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Modern Nontargeted Screening (NTS) Methods for Environmental Analysis 环境分析的现代非靶向筛选(NTS)方法
LCGC Europe Pub Date : 2022-04-01 DOI: 10.56530/10.56530/lcgc.eu.ay1289c2
S. Tisler, J. H. Christensen
{"title":"Modern Nontargeted Screening (NTS) Methods for Environmental Analysis","authors":"S. Tisler, J. H. Christensen","doi":"10.56530/10.56530/lcgc.eu.ay1289c2","DOIUrl":"https://doi.org/10.56530/10.56530/lcgc.eu.ay1289c2","url":null,"abstract":"LCGC Europe spoke to Selina Tisler and Jan H. Christensen from the University of Copenhagen in Denmark, about recent research projects focusing on nontargeted screening (NTS) approaches for important environmental monitoring applications, including the analysis of chemical leaching from plastic bottles using liquid chromatography tandem mass spectrometry (LC–MS/MS) and sediment analysis using comprehensive two-dimensional gas chromatography–high-resolution MS (GC×GC–HRMS).","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126763664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Supercritical Fluid Chromatography for Chiral Analysis, Part 1: Theoretical Background 超临界流体色谱手性分析，第1部分:理论背景
LCGC Europe Pub Date : 2022-03-01 DOI: 10.56530/lcgc.eu.ou1980m2
G. Roskam, Bas van de Velde, Andrea F. G. Gargano, Isabelle Kohler
{"title":"Supercritical Fluid Chromatography for Chiral Analysis, Part 1: Theoretical Background","authors":"G. Roskam, Bas van de Velde, Andrea F. G. Gargano, Isabelle Kohler","doi":"10.56530/lcgc.eu.ou1980m2","DOIUrl":"https://doi.org/10.56530/lcgc.eu.ou1980m2","url":null,"abstract":"The quantification of the enantiomers of racemic substances is of great importance in the development and regulation of pharmaceutical compounds. Active ingredients are often chiral; typically, only one of the stereoisomers has the desired pharmacokinetic and/or pharmacodynamic properties. Therefore, the stereoisomer distribution of chiral drug products must be characterized and evaluated during the drug discovery and development pipeline. Moreover, various chiral drugs present a stereoselective metabolism, highlighting the need for appropriate analytical strategies for the stereoselective analysis of metabolites, for example, in clinical and environmental studies. Due to its ease of use, robustness, and transferability, chiral liquid chromatography (LC) is the most common approach used in pharmaceutical analysis. Compared with LC, supercritical fluid chromatography (SFC) allows higher linear flow velocities while maintaining high chromatographic efficiency, often enabling the reduction of analysis time. In addition, SFC provides enhanced or complementary chiral selectivity and avoids or reduces toxic solvents, such as those used in normal-phase LC. In the first part of this review article the theoretical advantages, technological developments, and common practices in chiral SFC are discussed. This will be followed by a contribution discussing recent applications in pharmaceutical, clinical, forensic, and environmental analysis.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130053690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Hydrophilic-Phase Extraction: A New Avenue of Solid-Phase Extraction for Oligonucleotide Bioanalysis 亲水相萃取:寡核苷酸生物分析固相萃取的新途径
LCGC Europe Pub Date : 2022-02-01 DOI: 10.56530/lcgc.eu.kt3381t8
R. MacNeill
{"title":"Hydrophilic-Phase Extraction: A New Avenue of Solid-Phase Extraction for Oligonucleotide Bioanalysis","authors":"R. MacNeill","doi":"10.56530/lcgc.eu.kt3381t8","DOIUrl":"https://doi.org/10.56530/lcgc.eu.kt3381t8","url":null,"abstract":"A novel mode of bioanalytical solid-phase extraction (SPE) has been developed and used for a quantitative application involving an RNA oligonucleotide therapeutic candidate. Previously established protocols and media showed very little recovery, and so this new approach, based on hydrophilic interaction liquid chromatography (HILIC) with an aminopropyl-bonded phase on a silica support and providing an essentially uncharted avenue of selectivity, was explored. The procedure featured only one step prior to sample load, high-organic sorbent conditioning, two washes following the load that switched between low and high pH, followed by elution in an a highly eluotropic HILIC composition. The moniker of “hydrophilic-phase extraction” (HPE) was given to the procedure. The HPE was proven to be a critical part of a fully quantitative HILIC–high-resolution mass spectrometry (HRMS) method for the RNA oligonucleotide and has potential for general application.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"183 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114147587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
How Static Are Static Data? 静态数据有多静态?
LCGC Europe Pub Date : 2022-02-01 DOI: 10.56530/lcgc.eu.wy6473m6
R. Mcdowall
{"title":"How Static Are Static Data?","authors":"R. Mcdowall","doi":"10.56530/lcgc.eu.wy6473m6","DOIUrl":"https://doi.org/10.56530/lcgc.eu.wy6473m6","url":null,"abstract":"A balance printout is a fixed record, and is also called static data. But how static are static data when the weight is used in a chromatographic analysis? Also, have some regulatory data integrity guidance documents failed to comply with their own regulations?","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125020143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Analysis of Proteins, Biologics, and Nanoparticles in Biological Fluids Using Asymmetrical Flow Field-Flow Fractionation 使用不对称流场-流分馏分析生物流体中的蛋白质、生物制剂和纳米颗粒
LCGC Europe Pub Date : 2022-01-01 DOI: 10.56530/lcgc.eu.hv2689b6
M. Leeman, Alejandra Castro Nilsson, L. Nilsson
{"title":"Analysis of Proteins, Biologics, and Nanoparticles in Biological Fluids Using Asymmetrical Flow Field-Flow Fractionation","authors":"M. Leeman, Alejandra Castro Nilsson, L. Nilsson","doi":"10.56530/lcgc.eu.hv2689b6","DOIUrl":"https://doi.org/10.56530/lcgc.eu.hv2689b6","url":null,"abstract":"With the increasing interest in biopharmaceuticals such as proteins, antibodies, and nucleic acids, there is a corresponding increase in the need for characterizing such components. Much effort is spent on characterization in the early drug development phases as well as during formulation development and quality control. One parameter that is commonly investigated is the size distribution of the macromolecular components to deduce if there is aggregation or degradation occurring, if conformational changes occur, or if there are interactions with excipients. While the properties of the protein drug in the buffer system or in the pharmaceutical formulation are important, possibly even more interesting are the properties of the drug once it enters the body. Size characterization of macromolecules in biological fluids has traditionally been an area hampered by the complexity of the matrix. The large amount of indigenous components can interfere with commonly applied analytical techniques for size characterization. However, the separation technique asymmetrical flow field-flow fractionation (AF4) has recently shown increasing applicability for the characterization of components in blood plasma and serum. This article reviews some aspects of applying AF4 to plasma, serum, milk, and cerebrospinal fluid in the field of analysis and characterization of proteins, biologics, and nanoparticles in biological fluids.","PeriodicalId":402085,"journal":{"name":"LCGC Europe","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126174097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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